Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0001357

Physical Location & Seq

Genomic Coordinates Forward (+)

685879 .. 691132

Gene Structure 5254 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g01360.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1686 bp

ATGAGTCCCTCGCTGGTTCAAAACGGCGGCGTTTCGTCTCCGGCGCCACGTGTCGCCTCCGTTTACAGCGAGGTCCAGGCCAGCCGTATCGACCACGCGCTTCCTCTCCCCTCGGTGCTCAAAAAGCCGTTCAAGCTCATCGACGGACCTGCCAGCTCCGCCGCCGGCAATCCAGAGGAGATCGCCAAGCTATTTCCGAATGTTTTCGGTCAGCCCTCTGCACAGTTGGTGGAAAGTGACTCTGGTTCGGCGCTGCCAAATCAGAAGTTGAAGATTGGTGTGGTGTTGTCTGGAGGCCAAGCACCTGGAGGCCACAATGTGATCTCTGGAATTTTCGATTACTTGCAAGATCGTGCCCCGGGGAGCACATTGTATGGTTTCAGAGGGGGGCCTGCTGGTATCATGAAGGGAAAATATGTTGAACTTACACCGGAGTATGTTTATCCATACAGAAATCAGGGCGGTTTTGATATGATTTGTAGTGGAAGAGACAAGATTGAAACTCCAGAGCAGTTTAAGCAAGCACAAGAGACAGCTGTGAAGCTTGATTTGGATGGTCTTGTTGTTATTGGTGGAGATGACTCCAACACAAATGCATGTCTCCTTGCTGAAAACTTCAAGGGACTGAACTTGAAAACTCGGGTGATAGGTTGCCCAAAAACTATTGATGGTGATTTGAAATGTAAAGAGGTCCCCACGAGTTTCGGGTTCGATACAGCTTGCAAGATATATTCAGAAATGATTGGCAATGTCATGATAGATGCCCGGTCAACTGGAAAGTATTATCATTTTGTACGGCTTATGGGGCGTGCAGCTTCACACATTACATTAGAGTGTGCTTTGCAAACTCATCCAAATATCACAATTATTGGAGAAGAGGTTGCGGAAAAGAAGCAGACACTGAAAAATGTTACAGACTACATCGTAAACATAATCTTGAAGCGTGCTGATTTTGGTTATAACTACGGTGTTATACTTATTCCTGAAGGTCTTATTGACTTCATTCCTGAGGTGCAGGAGCTTATTGCTGAATTGAATGAAATTTTGGCCCATGATGTTGTAGATGAAGATGGGTTGTGGAAAAAGAAACTCACAAGTCAGTCTCTACAGCTTTTCGAATTACTACCTGGAGCAATCCAGGAGCAGTTGATGCTCGAAAGAGATCCTCATGGAAATGTTCAGGTTGCTAAGATAGAAACAGAGAAGATGCTTATCCAAATGGTTGAGGCTGACTTGGCAAAGAGGAAGCAGGAAGGTTCATACCATGGCCATTTCCAAGGAAAATCTCACTTTTTCGGGTATGAAGGAAGATGTGGTTTACCAACCAACTTTGATGCCAACTACTGCTATGCATTGGGTTTTGGTGCTGGAGCTCTCCTTCATGGTGGAAAGACTGGGTTGATATCTTCGGTTGGAAATTTGGCTGCCCCTGTTGAAGAATGGACAGTTGGTGGCACCGCATTGACTTCATTGATGGATGTGGAGAGGAGACACGGTAAGTTCAAGCCTGTGATCAAGAAGGCAATGGTAGAACTTGAAGCTGCTCCATTCAAGAAATTTGCATCTCTAAGGAATGAGTGGGCTATCAAGAATCGTTACATCAGTCCTGGTCCCATTCAATTTTTCGGCCCAGCTTCCAATTCCCTTAGTCACACACTAATCTTGGAACTCGGACTCGAAGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006793 GO:0006796 GO:0007275 GO:0008150 GO:0008152 GO:0008443 GO:0009719 GO:0009725 GO:0009735 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010154 GO:0015979 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019200 GO:0022414 GO:0030312 GO:0032501 GO:0032502 GO:0042221 GO:0044237 GO:0044238 GO:0044262 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0046835 GO:0047334 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0061458 GO:0071704 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00010 ko00030 ko00051 ko01100 ko01110 ko01120 ko01130 map00010 map00030 map00051 map01100 map01110 map01120 map01130

Pfam Domains

Protein Families

PFK

Protein Analysis

562

Amino Acids

61.02

Weight (kDa)

6.05

Isoelectric Point (pI)

37.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PFK	PF00365	91 - 353	7.4e-36	Phosphofructokinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001357)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G12000
fragaria_vesca	FvH4_3g01360 FvH4_3g01360 FvH4_3g22340 FvH4_5g38692
malus_domestica	MD05G1352800.v1.1 MD05G1354700.v1.1 MD05G1354900.v1.1 MD08G1183700.v1.1 MD09G1228400.v1.1 MD10G1133500.v1.1 MD10G1327400.v1.1 MD12G1211800.v1.1 MD13G1182600.v1.1 MD17G1176400.v1.1 MD17G1211800.v1.1
prunus_persica	Prupe.4G014600_v2.0.a1 Prupe.4G014600_v2.0.a1 Prupe.4G014600_v2.0.a1
pyrus_communis	pycom01g09830 pycom05g15580 pycom05g32000 pycom10g04820 pycom10g27690 pycom111g03470 pycom12g06510 pycom14g19720 pycom15g20870 pycom15g34000 pycom17g07230 pycom17g26290
rosa_chinensis	RchiOBHm_Chr1g0337721 RchiOBHm_Chr2g0111881 RchiOBHm_Chr2g0137981 RchiOBHm_Chr4g0422781 RchiOBHm_Chr5g0001981 RchiOBHm_Chr6g0245811 RchiOBHm_Chr7g0178941
rosa_laevigata	RLG00000030986
rosa_multiflora	Rmu_co8429625.1_g000001 Rmu_sc0000378.1_g000018 Rmu_sc0006008.1_g000007
rosa_roxburghii	Rroxscaffold_1G00071370 Rroxscaffold_1G00074370 Rroxscaffold_4G00281720
rosa_rugosa	Rorug02G0348600 Rorug04G0394100 Rorug04G0394200
rosa_samantha	Rh1AG166900 Rh2BG004000 Rh5CG016600 Rh5DG017200
rosa_wichuraiana	Rw5G001500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	960
Acc36I	ACCTGC	1 cut(s)	157
AccB1I	GGYRCC	2 cut(s)	43, 1454
AccII	CGCG	1 cut(s)	98
AciI	CCGC	6 cut(s)	27, 159, 162, 462, 884, 1458
AclWI	GGATC	1 cut(s)	1157
AcoI	YGGCCR	1 cut(s)	1267
AcsI	RAATTY	4 cut(s)	330, 1041, 1417, 1556
AcuI	CTGAAG	1 cut(s)	1005
AcvI	CACGTG	1 cut(s)	50
AcyI	GRCGYC	1 cut(s)	44
AdeI	CACNNNGTG	1 cut(s)	319
AfaI	GTAC	1 cut(s)	795
AflIII	ACRYGT	1 cut(s)	49
AjnI	CCWGG	5 cut(s)	75, 304, 1126, 1137, 1606
AjuI	GAANNNNNNNTTGG	6 cut(s)	533, 565, 1028, 1060, 1431, 1463
Alw21I	GWGCWC	3 cut(s)	120, 368, 1375
Alw26I	GTCTC	6 cut(s)	42, 483, 524, 605, 1107, 1483
AlwI	GGATC	1 cut(s)	1157
AlwNI	CAGNNNCTG	1 cut(s)	901
Ama87I	CYCGRG	2 cut(s)	358, 639
AoxI	GGCC	7 cut(s)	78, 295, 310, 389, 1047, 1267, 1627
ApeKI	GCWGC	4 cut(s)	253, 812, 1424, 1541
ApoI	RAATTY	4 cut(s)	330, 1041, 1417, 1556
Asp700I	GAANNNNTTC	1 cut(s)	203
AspLEI	GCGC	3 cut(s)	46, 100, 253
AspS9I	GGNCC	7 cut(s)	73, 146, 389, 691, 1048, 1610, 1628
AsuC2I	CCSGG	3 cut(s)	359, 360, 766
AsuHPI	GGTGA	2 cut(s)	655, 683
AsuII	TTCGAA	1 cut(s)	1116
AvaI	CYCGRG	2 cut(s)	358, 639
AvaII	GGWCC	4 cut(s)	73, 146, 691, 1610
AxyI	CCTNAGG	1 cut(s)	1008
BaeGI	GKGCMC	1 cut(s)	358
BalI	TGGCCA	1 cut(s)	1269
BanI	GGYRCC	2 cut(s)	43, 1454
BanII	GRGCYC	1 cut(s)	1375
BauI	CACGAG	1 cut(s)	697
BbrPI	CACGTG	1 cut(s)	50
Bbv12I	GWGCWC	3 cut(s)	120, 368, 1375
BbvI	GCAGC	4 cut(s)	240, 824, 1411, 1528
BccI	CCATC	4 cut(s)	548, 662, 1064, 1468
BceAI	ACGGC	4 cut(s)	40, 69, 112, 812
BcgI	CGANNNNNNTGC	2 cut(s)	131, 165
BciT130I	CCWGG	5 cut(s)	77, 306, 1128, 1139, 1608
BclI	TGATCA	1 cut(s)	1512
BcnI	CCSGG	3 cut(s)	359, 360, 766
BcoDI	GTCTC	6 cut(s)	42, 483, 524, 605, 1107, 1483
BfmI	CTRYAG	1 cut(s)	1106
BfoI	RGCGCY	2 cut(s)	47, 254
BfuAI	ACCTGC	1 cut(s)	157
BisI	GCNGC	6 cut(s)	28, 162, 254, 813, 1425, 1542
BlsI	GCNGC	6 cut(s)	29, 163, 255, 814, 1426, 1543
Bme1390I	CCNGG	8 cut(s)	77, 306, 359, 360, 766, 1128, 1139, 1608
Bme18I	GGWCC	4 cut(s)	73, 146, 691, 1610
BmeT110I	CYCGRG	2 cut(s)	358, 639
BmgT120I	GGNCC	7 cut(s)	73, 146, 389, 691, 1048, 1610, 1628
BmiI	GGNNCC	5 cut(s)	45, 390, 693, 1456, 1612
BmrFI	CCNGG	8 cut(s)	77, 306, 359, 360, 766, 1128, 1139, 1608
BmrI	ACTGGG	1 cut(s)	1404
BmsI	GCATC	5 cut(s)	751, 1140, 1197, 1324, 1571
BmuI	ACTGGG	1 cut(s)	1404
BpmI	CTGGAG	5 cut(s)	312, 327, 489, 1149, 1389
Bpu14I	TTCGAA	1 cut(s)	1116
BpuMI	CCSGG	3 cut(s)	359, 360, 766
BsaAI	YACGTR	1 cut(s)	50
BsaBI	GATNNNNATC	1 cut(s)	1211
BsaHI	GRCGYC	1 cut(s)	44
BsaJI	CCNNGG	6 cut(s)	111, 357, 358, 359, 1264, 1276
BsaWI	WCCGGW	1 cut(s)	430
BsaXI	ACNNNNNCTCC	4 cut(s)	864, 894, 1480, 1510
Bse118I	RCCGGY	1 cut(s)	164
Bse1I	ACTGG	2 cut(s)	778, 1399
Bse21I	CCTNAGG	1 cut(s)	1008
Bse3DI	GCAATG	2 cut(s)	754, 1530
Bse8I	GATNNNNATC	1 cut(s)	1211
BseBI	CCWGG	5 cut(s)	77, 306, 1128, 1139, 1608
BseDI	CCNNGG	6 cut(s)	111, 357, 358, 359, 1264, 1276
BseGI	GGATG	3 cut(s)	559, 850, 1483
BseJI	GATNNNNATC	1 cut(s)	1211
BseMI	GCAATG	2 cut(s)	754, 1530
BseMII	CTCAG	1 cut(s)	999
BseNI	ACTGG	2 cut(s)	778, 1399
BseRI	GAGGAG	2 cut(s)	191, 1501
BseSI	GKGCMC	1 cut(s)	358
BseXI	GCAGC	4 cut(s)	240, 824, 1411, 1528
BseYI	CCCAGC	1 cut(s)	1630
BsgI	GTGCAG	3 cut(s)	204, 831, 1034
Bsh1236I	CGCG	1 cut(s)	98
BshFI	GGCC	7 cut(s)	80, 297, 312, 391, 1049, 1269, 1629
BshNI	GGYRCC	2 cut(s)	43, 1454
BsiHKAI	GWGCWC	3 cut(s)	120, 368, 1375
BsiHKCI	CYCGRG	2 cut(s)	358, 639
BsiSI	CCGG	5 cut(s)	41, 165, 359, 431, 766
BslFI	GGGAC	3 cut(s)	636, 677, 1596
BsmAI	GTCTC	6 cut(s)	42, 483, 524, 605, 1107, 1483
BsmBI	CGTCTC	1 cut(s)	42
BsmFI	GGGAC	3 cut(s)	636, 677, 1596
BsnI	GGCC	7 cut(s)	80, 297, 312, 391, 1049, 1269, 1629
BsoBI	CYCGRG	2 cut(s)	358, 639
Bsp119I	TTCGAA	1 cut(s)	1116
Bsp1286I	GDGCHC	4 cut(s)	120, 358, 368, 1375
Bsp143I	GATC	5 cut(s)	180, 321, 349, 1162, 1512
Bsp19I	CCATGG	1 cut(s)	1264
BspACI	CCGC	6 cut(s)	27, 159, 162, 462, 884, 1458
BspANI	GGCC	7 cut(s)	80, 297, 312, 391, 1049, 1269, 1629
BspCNI	CTCAG	1 cut(s)	1000
BspFNI	CGCG	1 cut(s)	98
BspHI	TCATGA	2 cut(s)	402, 753
BspLI	GGNNCC	5 cut(s)	45, 390, 693, 1456, 1612
BspMI	ACCTGC	1 cut(s)	157
BspPI	GGATC	1 cut(s)	1157
BspT104I	TTCGAA	1 cut(s)	1116
BspT107I	GGYRCC	2 cut(s)	43, 1454
BsrDI	GCAATG	2 cut(s)	754, 1530
BsrFI	RCCGGY	1 cut(s)	164
BsrI	ACTGG	2 cut(s)	778, 1399
BssAI	RCCGGY	1 cut(s)	164
BssECI	CCNNGG	6 cut(s)	111, 357, 358, 359, 1264, 1276
BssMI	GATC	5 cut(s)	180, 321, 349, 1162, 1512
BssNI	GRCGYC	1 cut(s)	44
BssSI	CACGAG	1 cut(s)	697
BssT1I	CCWWGG	2 cut(s)	1264, 1276
Bst2BI	CACGAG	1 cut(s)	697
Bst2UI	CCWGG	5 cut(s)	77, 306, 1128, 1139, 1608
Bst4CI	ACNGT	4 cut(s)	225, 968, 1447, 1496
Bst6I	CTCTTC	2 cut(s)	481, 870
BstACI	GRCGYC	1 cut(s)	44
BstAPI	GCANNNNNTGC	1 cut(s)	1150
BstBAI	YACGTR	1 cut(s)	50
BstBI	TTCGAA	1 cut(s)	1116
BstC8I	GCNNGC	8 cut(s)	82, 154, 166, 393, 522, 721, 810, 945
BstDEI	CTNAG	4 cut(s)	1008, 1188, 1568, 1646
BstDSI	CCRYGG	1 cut(s)	1264
BstF5I	GGATG	3 cut(s)	559, 850, 1483
BstFNI	CGCG	1 cut(s)	98
BstH2I	RGCGCY	2 cut(s)	47, 254
BstHHI	GCGC	3 cut(s)	46, 100, 253
BstKTI	GATC	5 cut(s)	183, 324, 352, 1165, 1515
BstMAI	GTCTC	6 cut(s)	42, 483, 524, 605, 1107, 1483
BstMBI	GATC	5 cut(s)	180, 321, 349, 1162, 1512
BstMWI	GCNNNNNNNGC	7 cut(s)	124, 133, 158, 517, 805, 1150, 1235
BstNI	CCWGG	5 cut(s)	77, 306, 1128, 1139, 1608
BstNSI	RCATGY	1 cut(s)	600
BstSCI	CCNGG	8 cut(s)	75, 304, 357, 358, 764, 1126, 1137, 1606
BstSFI	CTRYAG	1 cut(s)	1106
BstSLI	GKGCMC	1 cut(s)	358
BstUI	CGCG	1 cut(s)	98
BstV1I	GCAGC	4 cut(s)	240, 824, 1411, 1528
BstX2I	RGATCY	1 cut(s)	1162
BstXI	CCANNNNNNTGG	1 cut(s)	305
BstYI	RGATCY	1 cut(s)	1162
Bsu36I	CCTNAGG	1 cut(s)	1008
BsuRI	GGCC	7 cut(s)	80, 297, 312, 391, 1049, 1269, 1629
BtgI	CCRYGG	1 cut(s)	1264
BtsCI	GGATG	3 cut(s)	559, 850, 1483
BtsIMutI	CAGTG	1 cut(s)	899
BveI	ACCTGC	1 cut(s)	157
Cac8I	GCNNGC	8 cut(s)	82, 154, 166, 393, 522, 721, 810, 945
CaiI	CAGNNNCTG	1 cut(s)	901
CciI	TCATGA	2 cut(s)	402, 753
CfoI	GCGC	3 cut(s)	46, 100, 253
Cfr10I	RCCGGY	1 cut(s)	164
Cfr13I	GGNCC	7 cut(s)	73, 146, 389, 691, 1048, 1610, 1628
Cfr9I	CCCGGG	1 cut(s)	358
Csp6I	GTAC	1 cut(s)	794
CviAII	CATG	7 cut(s)	403, 597, 754, 1052, 1169, 1265, 1382
CviQI	GTAC	1 cut(s)	794
DdeI	CTNAG	4 cut(s)	1008, 1188, 1568, 1646
DinI	GGCGCC	1 cut(s)	45
DpnI	GATC	5 cut(s)	182, 323, 351, 1164, 1514
DpnII	GATC	5 cut(s)	180, 321, 349, 1162, 1512
DraIII	CACNNNGTG	1 cut(s)	319
EaeI	YGGCCR	1 cut(s)	1267
Eam1104I	CTCTTC	2 cut(s)	481, 870
EarI	CTCTTC	2 cut(s)	481, 870
EciI	GGCGGA	1 cut(s)	148
Ecl136II	GAGCTC	1 cut(s)	1373
Eco130I	CCWWGG	2 cut(s)	1264, 1276
Eco24I	GRGCYC	1 cut(s)	1375
Eco32I	GATATC	1 cut(s)	1404
Eco47I	GGWCC	4 cut(s)	73, 146, 691, 1610
Eco53kI	GAGCTC	1 cut(s)	1373
Eco57I	CTGAAG	1 cut(s)	1005
Eco72I	CACGTG	1 cut(s)	50
Eco81I	CCTNAGG	1 cut(s)	1008
Eco88I	CYCGRG	2 cut(s)	358, 639
EcoICRI	GAGCTC	1 cut(s)	1373
EcoO109I	RGGNCCY	2 cut(s)	389, 691
EcoRII	CCWGG	5 cut(s)	75, 304, 1126, 1137, 1606
EcoRV	GATATC	1 cut(s)	1404
EcoT14I	CCWWGG	2 cut(s)	1264, 1276
EcoT22I	ATGCAT	2 cut(s)	598, 1354
EcoT38I	GRGCYC	1 cut(s)	1375
EgeI	GGCGCC	1 cut(s)	45
EheI	GGCGCC	1 cut(s)	45
ErhI	CCWWGG	2 cut(s)	1264, 1276
Esp3I	CGTCTC	1 cut(s)	42
FaeI	CATG	7 cut(s)	406, 600, 757, 1055, 1172, 1268, 1385
FaqI	GGGAC	3 cut(s)	636, 677, 1596
FatI	CATG	7 cut(s)	402, 596, 753, 1051, 1168, 1264, 1381
FbaI	TGATCA	1 cut(s)	1512
Fnu4HI	GCNGC	6 cut(s)	28, 162, 254, 813, 1425, 1542
FokI	GGATG	3 cut(s)	566, 837, 1490
FriOI	GRGCYC	1 cut(s)	1375
Fsp4HI	GCNGC	6 cut(s)	28, 162, 254, 813, 1425, 1542
GlaI	GCGC	3 cut(s)	45, 99, 252
GluI	GCNGC	6 cut(s)	28, 162, 254, 813, 1425, 1542
GsaI	CCCAGC	1 cut(s)	1634
GsuI	CTGGAG	5 cut(s)	312, 327, 489, 1149, 1389
HaeII	RGCGCY	2 cut(s)	47, 254
HaeIII	GGCC	7 cut(s)	80, 297, 312, 391, 1049, 1269, 1629
HapII	CCGG	5 cut(s)	41, 165, 359, 431, 766
HhaI	GCGC	3 cut(s)	46, 100, 253
Hin1I	GRCGYC	1 cut(s)	44
Hin1II	CATG	7 cut(s)	406, 600, 757, 1055, 1172, 1268, 1385
Hin6I	GCGC	3 cut(s)	44, 98, 251
HinP1I	GCGC	3 cut(s)	44, 98, 251
HincII	GTYRAC	1 cut(s)	771
HindII	GTYRAC	1 cut(s)	771
HindIII	AAGCTT	1 cut(s)	542
HinfI	GANTC	5 cut(s)	4, 239, 581, 1591, 1674
HpaII	CCGG	5 cut(s)	41, 165, 359, 431, 766
HphI	GGTGA	2 cut(s)	655, 683
Hpy166II	GTNNAC	4 cut(s)	64, 771, 928, 1319
Hpy188I	TCNGA	5 cut(s)	198, 264, 383, 736, 1673
Hpy8I	GTNNAC	4 cut(s)	64, 771, 928, 1319
Hpy99I	CGWCG	1 cut(s)	146
HpyAV	CCTTC	6 cut(s)	400, 980, 1247, 1298, 1388, 1513
HpyCH4III	ACNGT	4 cut(s)	225, 968, 1447, 1496
HpyCH4IV	ACGT	1 cut(s)	49
HpyCH4V	TGCA	9 cut(s)	221, 346, 596, 723, 812, 844, 1015, 1352, 1562
HpyF10VI	GCNNNNNNNGC	7 cut(s)	124, 133, 158, 517, 805, 1150, 1235
HpyF3I	CTNAG	4 cut(s)	1008, 1188, 1568, 1646
HpySE526I	ACGT	1 cut(s)	49
Hsp92I	GRCGYC	1 cut(s)	44
Hsp92II	CATG	7 cut(s)	406, 600, 757, 1055, 1172, 1268, 1385
HspAI	GCGC	3 cut(s)	44, 98, 251
KasI	GGCGCC	1 cut(s)	43
KroI	GCCGGC	1 cut(s)	164
KroNI	GCCGGC	1 cut(s)	166
Ksp22I	TGATCA	1 cut(s)	1512
Kzo9I	GATC	5 cut(s)	180, 321, 349, 1162, 1512
LmnI	GCTCC	7 cut(s)	161, 363, 1018, 1130, 1141, 1370, 1549
Lsp1109I	GCAGC	4 cut(s)	240, 824, 1411, 1528
LweI	GCATC	5 cut(s)	751, 1140, 1197, 1324, 1571
MaeII	ACGT	1 cut(s)	49
MaeIII	GTNAC	4 cut(s)	236, 910, 1595, 1649
MalI	GATC	5 cut(s)	182, 323, 351, 1164, 1514
MboI	GATC	5 cut(s)	180, 321, 349, 1162, 1512
MboII	GAAGA	8 cut(s)	283, 498, 887, 1079, 1216, 1320, 1398, 1448
MflI	RGATCY	1 cut(s)	1162
MhlI	GDGCHC	4 cut(s)	120, 358, 368, 1375
MlsI	TGGCCA	1 cut(s)	1269
MluCI	AATT	9 cut(s)	330, 864, 1031, 1041, 1118, 1417, 1556, 1619, 1639
MluNI	TGGCCA	1 cut(s)	1269
Mly113I	GGCGCC	1 cut(s)	44
MlyI	GAGTC	4 cut(s)	13, 233, 575, 1668
MmeI	TCCRAC	2 cut(s)	609, 1393
Mox20I	TGGCCA	1 cut(s)	1269
Mph1103I	ATGCAT	2 cut(s)	598, 1354
MroNI	GCCGGC	1 cut(s)	164
MroXI	GAANNNNTTC	1 cut(s)	203
MscI	TGGCCA	1 cut(s)	1269
MseI	TTAA	2 cut(s)	516, 1684
MslI	CAYNNNNRTG	2 cut(s)	832, 1173
Msp20I	TGGCCA	1 cut(s)	1269
MspA1I	CMGCKG	1 cut(s)	536
MspI	CCGG	5 cut(s)	41, 165, 359, 431, 766
MspR9I	CCNGG	8 cut(s)	77, 306, 359, 360, 766, 1128, 1139, 1608
MvaI	CCWGG	5 cut(s)	77, 306, 1128, 1139, 1608
MvnI	CGCG	1 cut(s)	98
MwoI	GCNNNNNNNGC	7 cut(s)	124, 133, 158, 517, 805, 1150, 1235
NaeI	GCCGGC	1 cut(s)	166
NarI	GGCGCC	1 cut(s)	44
NciI	CCSGG	3 cut(s)	359, 360, 766
NcoI	CCATGG	1 cut(s)	1264
NdeII	GATC	5 cut(s)	180, 321, 349, 1162, 1512
NgoMIV	GCCGGC	1 cut(s)	164
NlaIII	CATG	7 cut(s)	406, 600, 757, 1055, 1172, 1268, 1385
NlaIV	GGNNCC	5 cut(s)	45, 390, 693, 1456, 1612
NmuCI	GTSAC	2 cut(s)	236, 1649
NsiI	ATGCAT	2 cut(s)	598, 1354
NspI	RCATGY	1 cut(s)	600
NspV	TTCGAA	1 cut(s)	1116
PagI	TCATGA	2 cut(s)	402, 753
PdiI	GCCGGC	1 cut(s)	166
PdmI	GAANNNNTTC	1 cut(s)	203
PfeI	GAWTC	1 cut(s)	1591
PfoI	TCCNGGA	1 cut(s)	1137
PkrI	GCNGC	6 cut(s)	29, 163, 255, 814, 1426, 1543
PleI	GAGTC	4 cut(s)	12, 233, 575, 1668
PluTI	GGCGCC	1 cut(s)	47
PmaCI	CACGTG	1 cut(s)	50
PmlI	CACGTG	1 cut(s)	50
PpsI	GAGTC	4 cut(s)	12, 233, 575, 1668
Ppu21I	YACGTR	1 cut(s)	50
PpuMI	RGGWCCY	1 cut(s)	691
PsiI	TTATAA	1 cut(s)	960
Psp124BI	GAGCTC	1 cut(s)	1375
Psp5II	RGGWCCY	1 cut(s)	691
Psp6I	CCWGG	5 cut(s)	75, 304, 1126, 1137, 1606
PspCI	CACGTG	1 cut(s)	50
PspFI	CCCAGC	1 cut(s)	1630
PspGI	CCWGG	5 cut(s)	75, 304, 1126, 1137, 1606
PspN4I	GGNNCC	5 cut(s)	45, 390, 693, 1456, 1612
PspPI	GGNCC	7 cut(s)	73, 146, 389, 691, 1048, 1610, 1628
PspPPI	RGGWCCY	1 cut(s)	691
PstNI	CAGNNNCTG	1 cut(s)	901
PsuI	RGATCY	1 cut(s)	1162
PvuII	CAGCTG	1 cut(s)	536
RsaI	GTAC	1 cut(s)	795
RsaNI	GTAC	1 cut(s)	794
RseI	CAYNNNNRTG	2 cut(s)	832, 1173
SacI	GAGCTC	1 cut(s)	1375
SaqAI	TTAA	2 cut(s)	516, 1684
SatI	GCNGC	6 cut(s)	28, 162, 254, 813, 1425, 1542
Sau3AI	GATC	5 cut(s)	180, 321, 349, 1162, 1512
Sau96I	GGNCC	7 cut(s)	73, 146, 389, 691, 1048, 1610, 1628
SchI	GAGTC	4 cut(s)	13, 233, 575, 1668
ScrFI	CCNGG	8 cut(s)	77, 306, 359, 360, 766, 1128, 1139, 1608
SduI	GDGCHC	4 cut(s)	120, 358, 368, 1375
SfaNI	GCATC	5 cut(s)	751, 1140, 1197, 1324, 1571
SfcI	CTRYAG	1 cut(s)	1106
SfoI	GGCGCC	1 cut(s)	45
SfuI	TTCGAA	1 cut(s)	1116
SinI	GGWCC	4 cut(s)	73, 146, 691, 1610
SmaI	CCCGGG	1 cut(s)	360
SmiMI	CAYNNNNRTG	2 cut(s)	832, 1173
Sse9I	AATT	9 cut(s)	330, 864, 1031, 1041, 1118, 1417, 1556, 1619, 1639
SsiI	CCGC	6 cut(s)	27, 159, 162, 462, 884, 1458
SspDI	GGCGCC	1 cut(s)	43
SstI	GAGCTC	1 cut(s)	1375
StyD4I	CCNGG	8 cut(s)	75, 304, 357, 358, 764, 1126, 1137, 1606
StyI	CCWWGG	2 cut(s)	1264, 1276
TaaI	ACNGT	4 cut(s)	225, 968, 1447, 1496
TaiI	ACGT	1 cut(s)	52
TaqI	TCGA	7 cut(s)	90, 141, 336, 711, 1116, 1155, 1677
TaqII	GACCGA	1 cut(s)	197
TasI	AATT	9 cut(s)	330, 864, 1031, 1041, 1118, 1417, 1556, 1619, 1639
TauI	GCSGC	2 cut(s)	30, 164
TfiI	GAWTC	1 cut(s)	1591
Tru1I	TTAA	2 cut(s)	516, 1684
Tru9I	TTAA	2 cut(s)	516, 1684
TscAI	CASTG	1 cut(s)	906
TseFI	GTSAC	2 cut(s)	236, 1649
TseI	GCWGC	4 cut(s)	253, 812, 1424, 1541
Tsp45I	GTSAC	2 cut(s)	236, 1649
TspDTI	ATGAA	8 cut(s)	419, 991, 1053, 1080, 1248, 1317, 1370, 1458
TspGWI	ACGGA	2 cut(s)	49, 159
TspMI	CCCGGG	1 cut(s)	358
TspRI	CASTG	1 cut(s)	906
VpaK11BI	GGWCC	4 cut(s)	73, 146, 691, 1610
XapI	RAATTY	4 cut(s)	330, 1041, 1417, 1556
XceI	RCATGY	1 cut(s)	600
XmaI	CCCGGG	1 cut(s)	358
XmnI	GAANNNNTTC	1 cut(s)	203
Zsp2I	ATGCAT	2 cut(s)	598, 1354

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.