Rorug02G0348600

Protein of unknown function (DUF632)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001357
Physical Location & Seq
Reverse (-)
43695919 .. 43716931
21013 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0348600.1

Sequence Viewer

Length: 1488 bp
ATGGTACAGAGTGGTTTGGACACAGAAAAGGTTGAGAATTCAGGAGCCAAATTCACTCAGAGTGGAAGCCAAGCATCAGGTCTTCACAGGGATCCCTCATTCTCTCGCTGGTTTGATGAAGATGGGAGAGTGCAAGGTGATTTGGTAAATACGGATGCGACGGTAGAAGAAGACCCTGAATTTGAATTGCCTCTGCCCCAACAGGGAGAGCTAGAAAACAAGGGTTTAGATATGGACAGGGACCGTATATCTCCAAGGGGAAGTAAGCGCTTGAACAGTGGTGATACCTTTGGTAGTGCTACTGTCCAGCTAGATGGAAATGATCAAGCAAAGCTTTTGCCTTTTGACGTTGAGAAGGGGTCAGAAAGGGAGCTACAGGACATAGATCACTCTACTTCTGTTGATGACCACAAGGCATTATCCTCTAATTCAAAGTATCCCATTTCTTCTGCAATTGTACTGAAAACATTGTTCTTTGTACTTGTATGGTACACTTGCAGCCTATTTTTGACCTTGTATAACAAAAGTCTGTTAGGAGATAATTTGGGAAAGTTCCCCGCTCCCTTATTGATGAATACTGTTCACTTTACAATGCAAGCTATTTTATCAAAGGCAATCACGTGGTACTGGTCTCATAGGTTCAAAACCAGTGTCACTATGTCTTGGAGAGATTATTTTGCTAAAGTTGTACCAACGGCTCTTGGAACAGCAATGGATGTTAATCTAAGCAATGCATCCCTTGTTTTTATATCTGTCACATTTGCCACAATGTGTAAATCCGCGGCTCCAATATTTCTCCTTCTATTTGCTTTCGCTTTCAGGTTGGAATCTCCGAGCTATAAACTTTCGGGAATCATATTGGTTATCTCCATTGGAATACTACTAACAGTTGCAAAGGAGACAGAATTTGATTTCTGGGGATTCATTTTTGTCATGCTTGCTGCTGTTATGTCTGGTTTCCGATGGTGTATGACTCAAATTCTTCTTCAGAAAGAATCATATGGTTTGAAAAATCCACTTACTTTGATGAGCTATGTGACTCCGGTTATGGCAGTGGTGACTGCCCTGCTCTCTTTTATTTTTGATCCATGGGATGAACTTAGAAGAAACAATTACTTCAATAATCCTGAGCATATCAGTCGAAGTTGCTTGCTGATGCTTTTTGGTGGAACCCTTGCCTTTTTCATGGTATTAACAGAATTCATTCTTGTCTCAGTGACCAGTGCTGTCACTGTCACAATCGCAGGTGTTGTCAAGGAGGCTCTCACTATATTGGTTGCAGTCATTTACTTCCATGATGAATTTACTATGTTAAAAGGAGTTGGACTCTTCACAATTATTCTTGGGGTGAGTTTATTCAACTGGTACAAGTATGAAAAACTAAAGAAGGGTCATACAAGTGAAGACGACATGGAGGAATCAGTCGTGATGAATGCTGCAAAATATGTTATTCTTGAAGAGACAGATGAGCAAGATGATGGCACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF630 DUF632

Protein Analysis

495

Amino Acids

55.41

Weight (kDa)

4.97

Isoelectric Point (pI)

37.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPT PF03151 155 - 454 3.9e-28 Triose-phosphate Transporter family
UAA PF08449 192 - 454 5.9e-08 UAA transporter family
PUNUT PF16913 215 - 462 3.1e-06 Purine nucleobase transmembrane transport
EamA PF00892 306 - 454 4.9e-06 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1235
Acc36I ACCTGC 1 cut(s) 1235
AccBSI CCGCTC 1 cut(s) 560
AccII CGCG 1 cut(s) 782
AciI CCGC 3 cut(s) 558, 780, 782
AclWI GGATC 3 cut(s) 86, 99, 1079
AcsI RAATTY 7 cut(s) 37, 50, 179, 905, 978, 1199, 1301
AcuI CTGAAG 1 cut(s) 971
AcvI CACGTG 1 cut(s) 621
AdeI CACNNNGTG 1 cut(s) 771
AfaI GTAC 7 cut(s) 6, 459, 480, 491, 626, 690, 1367
AfeI AGCGCT 1 cut(s) 269
AfiI CCNNNNNNNGG 1 cut(s) 203
AgsI TTSAA 8 cut(s) 185, 274, 432, 643, 1009, 1120, 1360, 1457
AluBI AGCT 7 cut(s) 211, 310, 334, 373, 599, 837, 1032
AluI AGCT 7 cut(s) 211, 310, 334, 373, 599, 837, 1032
Alw26I GTCTC 4 cut(s) 636, 893, 1216, 1454
AlwI GGATC 3 cut(s) 86, 99, 1079
Aor51HI AGCGCT 1 cut(s) 269
ApeKI GCWGC 3 cut(s) 498, 941, 1436
ApoI RAATTY 7 cut(s) 37, 50, 179, 905, 978, 1199, 1301
ArsI GACNNNNNNTTYG 4 cut(s) 164, 196, 636, 668
Asp700I GAANNNNTTC 1 cut(s) 1203
AspLEI GCGC 1 cut(s) 270
AspS9I GGNCC 1 cut(s) 241
AsuHPI GGTGA 4 cut(s) 149, 293, 1069, 1360
AvaII GGWCC 1 cut(s) 241
BamHI GGATCC 1 cut(s) 91
BbrPI CACGTG 1 cut(s) 621
BbsI GAAGAC 3 cut(s) 74, 177, 1410
BbvI GCAGC 3 cut(s) 510, 928, 1423
BccI CCATC 4 cut(s) 116, 308, 957, 1472
BceAI ACGGC 1 cut(s) 711
BcgI CGANNNNNNTGC 2 cut(s) 1121, 1155
BciVI GTATCC 1 cut(s) 447
BclI TGATCA 1 cut(s) 322
BcoDI GTCTC 4 cut(s) 636, 893, 1216, 1454
BfaI CTAG 2 cut(s) 212, 311
BfmI CTRYAG 1 cut(s) 374
BfoI RGCGCY 1 cut(s) 271
BfuAI ACCTGC 1 cut(s) 1235
BfuI GTATCC 1 cut(s) 447
BisI GCNGC 4 cut(s) 499, 783, 942, 1437
BlsI GCNGC 4 cut(s) 500, 784, 943, 1438
Bme18I GGWCC 1 cut(s) 241
BmgT120I GGNCC 1 cut(s) 241
BmiI GGNNCC 5 cut(s) 46, 93, 242, 786, 1171
BmsI GCATC 4 cut(s) 83, 145, 743, 1146
BpiI GAAGAC 3 cut(s) 74, 177, 1410
BplI GAGNNNNNCTC 2 cut(s) 1311, 1343
Bpu10I CCTNAGC 1 cut(s) 1128
BsaAI YACGTR 1 cut(s) 621
BsaBI GATNNNNATC 1 cut(s) 720
BsaI GGTCTC 1 cut(s) 636
BsaJI CCNNGG 3 cut(s) 254, 780, 1088
BsaWI WCCGGW 1 cut(s) 1042
Bsc4I CCNNNNNNNGG 1 cut(s) 203
Bse1I ACTGG 4 cut(s) 632, 648, 1221, 1367
Bse3DI GCAATG 2 cut(s) 717, 736
Bse8I GATNNNNATC 1 cut(s) 720
BseDI CCNNGG 3 cut(s) 254, 780, 1088
BseGI GGATG 4 cut(s) 160, 721, 734, 1099
BseJI GATNNNNATC 1 cut(s) 720
BseLI CCNNNNNNNGG 1 cut(s) 203
BseMI GCAATG 2 cut(s) 717, 736
BseMII CTCAG 3 cut(s) 71, 1119, 1227
BseNI ACTGG 4 cut(s) 632, 648, 1221, 1367
BseXI GCAGC 3 cut(s) 510, 928, 1423
Bsh1236I CGCG 1 cut(s) 782
BsiSI CCGG 1 cut(s) 1043
BslFI GGGAC 1 cut(s) 254
BslI CCNNNNNNNGG 1 cut(s) 203
BsmAI GTCTC 4 cut(s) 636, 893, 1216, 1454
BsmFI GGGAC 1 cut(s) 254
BsmI GAATGC 1 cut(s) 1438
Bso31I GGTCTC 1 cut(s) 636
Bsp143I GATC 4 cut(s) 91, 322, 385, 1084
Bsp19I CCATGG 1 cut(s) 1088
BspACI CCGC 3 cut(s) 558, 780, 782
BspCNI CTCAG 3 cut(s) 70, 1120, 1226
BspFNI CGCG 1 cut(s) 782
BspLI GGNNCC 5 cut(s) 46, 93, 242, 786, 1171
BspMI ACCTGC 1 cut(s) 1235
BspPI GGATC 3 cut(s) 86, 99, 1079
BspTNI GGTCTC 1 cut(s) 636
BsrBI CCGCTC 1 cut(s) 560
BsrDI GCAATG 2 cut(s) 717, 736
BsrI ACTGG 4 cut(s) 632, 648, 1221, 1367
BssECI CCNNGG 3 cut(s) 254, 780, 1088
BssMI GATC 4 cut(s) 91, 322, 385, 1084
BssT1I CCWWGG 2 cut(s) 254, 1088
Bst4CI ACNGT 7 cut(s) 163, 245, 278, 304, 580, 889, 1234
Bst6I CTCTTC 2 cut(s) 1334, 1452
BstBAI YACGTR 1 cut(s) 621
BstC8I GCNNGC 3 cut(s) 597, 939, 1151
BstDEI CTNAG 5 cut(s) 57, 725, 1100, 1128, 1213
BstDSI CCRYGG 2 cut(s) 780, 1088
BstF5I GGATG 4 cut(s) 160, 721, 734, 1099
BstFNI CGCG 1 cut(s) 782
BstH2I RGCGCY 1 cut(s) 271
BstHHI GCGC 1 cut(s) 270
BstKTI GATC 4 cut(s) 94, 325, 388, 1087
BstMAI GTCTC 4 cut(s) 636, 893, 1216, 1454
BstMBI GATC 4 cut(s) 91, 322, 385, 1084
BstSFI CTRYAG 1 cut(s) 374
BstUI CGCG 1 cut(s) 782
BstV1I GCAGC 3 cut(s) 510, 928, 1423
BstV2I GAAGAC 3 cut(s) 74, 177, 1410
BstX2I RGATCY 1 cut(s) 91
BstXI CCANNNNNNTGG 1 cut(s) 314
BstYI RGATCY 1 cut(s) 91
BsuI GTATCC 1 cut(s) 447
BtgI CCRYGG 2 cut(s) 780, 1088
BtsCI GGATG 4 cut(s) 160, 721, 734, 1099
BtsI GCAGTG 1 cut(s) 1059
BtsIMutI CAGTG 6 cut(s) 283, 655, 1059, 1221, 1228, 1230
BveI ACCTGC 1 cut(s) 1235
Cac8I GCNNGC 3 cut(s) 597, 939, 1151
CfoI GCGC 1 cut(s) 270
Cfr13I GGNCC 1 cut(s) 241
Cfr42I CCGCGG 1 cut(s) 783
Csp6I GTAC 7 cut(s) 5, 458, 479, 490, 625, 689, 1366
CspCI CAANNNNNGTGG 2 cut(s) 1005, 1040
CviAII CATG 5 cut(s) 934, 1089, 1186, 1295, 1411
CviQI GTAC 7 cut(s) 5, 458, 479, 490, 625, 689, 1366
DdeI CTNAG 5 cut(s) 57, 725, 1100, 1128, 1213
DpnI GATC 4 cut(s) 93, 324, 387, 1086
DpnII GATC 4 cut(s) 91, 322, 385, 1084
DraIII CACNNNGTG 1 cut(s) 771
Eam1104I CTCTTC 2 cut(s) 1334, 1452
EarI CTCTTC 2 cut(s) 1334, 1452
Eco130I CCWWGG 2 cut(s) 254, 1088
Eco31I GGTCTC 1 cut(s) 636
Eco47I GGWCC 1 cut(s) 241
Eco47III AGCGCT 1 cut(s) 269
Eco57I CTGAAG 1 cut(s) 971
Eco72I CACGTG 1 cut(s) 621
EcoRI GAATTC 2 cut(s) 37, 1199
EcoT14I CCWWGG 2 cut(s) 254, 1088
EcoT22I ATGCAT 1 cut(s) 736
ErhI CCWWGG 2 cut(s) 254, 1088
FaeI CATG 5 cut(s) 937, 1092, 1189, 1298, 1414
FalI AAGNNNNNCTT 2 cut(s) 318, 350
FaqI GGGAC 1 cut(s) 254
FatI CATG 5 cut(s) 933, 1088, 1185, 1294, 1410
FauI CCCGC 1 cut(s) 565
FauNDI CATATG 1 cut(s) 1000
FbaI TGATCA 1 cut(s) 322
Fnu4HI GCNGC 4 cut(s) 499, 783, 942, 1437
FokI GGATG 4 cut(s) 167, 721, 728, 1106
Fsp4HI GCNGC 4 cut(s) 499, 783, 942, 1437
FspBI CTAG 2 cut(s) 212, 311
GlaI GCGC 1 cut(s) 269
GluI GCNGC 4 cut(s) 499, 783, 942, 1437
HaeII RGCGCY 1 cut(s) 271
HapII CCGG 1 cut(s) 1043
HhaI GCGC 1 cut(s) 270
Hin1II CATG 5 cut(s) 937, 1092, 1189, 1298, 1414
Hin6I GCGC 1 cut(s) 268
HinP1I GCGC 1 cut(s) 268
HindIII AAGCTT 1 cut(s) 332
HinfI GANTC 8 cut(s) 827, 852, 921, 973, 995, 1039, 1326, 1418
HpaII CCGG 1 cut(s) 1043
HphI GGTGA 4 cut(s) 149, 293, 1069, 1360
Hpy166II GTNNAC 2 cut(s) 492, 583
Hpy188I TCNGA 5 cut(s) 60, 364, 834, 962, 990
Hpy188III TCNNGA 5 cut(s) 42, 849, 1127, 1426, 1454
Hpy8I GTNNAC 2 cut(s) 492, 583
Hpy99I CGWCG 1 cut(s) 163
HpyAV CCTTC 3 cut(s) 349, 809, 1381
HpyCH4III ACNGT 7 cut(s) 163, 245, 278, 304, 580, 889, 1234
HpyCH4IV ACGT 2 cut(s) 348, 620
HpyCH4V TGCA 8 cut(s) 133, 452, 498, 595, 734, 893, 1280, 1439
HpyF3I CTNAG 5 cut(s) 57, 725, 1100, 1128, 1213
HpySE526I ACGT 2 cut(s) 348, 620
Hsp92II CATG 5 cut(s) 937, 1092, 1189, 1298, 1414
HspAI GCGC 1 cut(s) 268
Ksp22I TGATCA 1 cut(s) 322
KspI CCGCGG 1 cut(s) 783
Kzo9I GATC 4 cut(s) 91, 322, 385, 1084
LmnI GCTCC 4 cut(s) 44, 370, 565, 790
Lsp1109I GCAGC 3 cut(s) 510, 928, 1423
LweI GCATC 4 cut(s) 83, 145, 743, 1146
MaeI CTAG 2 cut(s) 212, 311
MaeII ACGT 2 cut(s) 348, 620
MaeIII GTNAC 7 cut(s) 652, 754, 1036, 1057, 1216, 1228, 1234
MalI GATC 4 cut(s) 93, 324, 387, 1086
MbiI CCGCTC 1 cut(s) 560
MboI GATC 4 cut(s) 91, 322, 385, 1084
MfeI CAATTG 1 cut(s) 453
MflI RGATCY 1 cut(s) 91
MlyI GAGTC 3 cut(s) 967, 1033, 1320
MmeI TCCRAC 2 cut(s) 804, 1303
MnlI CCTC 5 cut(s) 106, 201, 433, 1252, 1408
Mph1103I ATGCAT 1 cut(s) 736
MroXI GAANNNNTTC 1 cut(s) 1203
MseI TTAA 3 cut(s) 720, 1193, 1313
MslI CAYNNNNRTG 1 cut(s) 1398
MspA1I CMGCKG 1 cut(s) 782
MspI CCGG 1 cut(s) 1043
MunI CAATTG 1 cut(s) 453
Mva1269I GAATGC 1 cut(s) 1438
MvnI CGCG 1 cut(s) 782
NcoI CCATGG 1 cut(s) 1088
NdeI CATATG 1 cut(s) 1000
NdeII GATC 4 cut(s) 91, 322, 385, 1084
NlaIII CATG 5 cut(s) 937, 1092, 1189, 1298, 1414
NlaIV GGNNCC 5 cut(s) 46, 93, 242, 786, 1171
NmuCI GTSAC 7 cut(s) 652, 754, 1036, 1057, 1216, 1228, 1234
NsiI ATGCAT 1 cut(s) 736
PaqCI CACCTGC 1 cut(s) 1235
PctI GAATGC 1 cut(s) 1438
PdmI GAANNNNTTC 1 cut(s) 1203
PfeI GAWTC 5 cut(s) 827, 852, 921, 995, 1418
PkrI GCNGC 4 cut(s) 500, 784, 943, 1438
PleI GAGTC 3 cut(s) 967, 1033, 1320
PmaCI CACGTG 1 cut(s) 621
PmlI CACGTG 1 cut(s) 621
PpsI GAGTC 3 cut(s) 967, 1033, 1320
Ppu21I YACGTR 1 cut(s) 621
PspCI CACGTG 1 cut(s) 621
PspN4I GGNNCC 5 cut(s) 46, 93, 242, 786, 1171
PspPI GGNCC 1 cut(s) 241
PsuI RGATCY 1 cut(s) 91
RsaI GTAC 7 cut(s) 6, 459, 480, 491, 626, 690, 1367
RsaNI GTAC 7 cut(s) 5, 458, 479, 490, 625, 689, 1366
RseI CAYNNNNRTG 1 cut(s) 1398
SacII CCGCGG 1 cut(s) 783
SaqAI TTAA 3 cut(s) 720, 1193, 1313
SatI GCNGC 4 cut(s) 499, 783, 942, 1437
Sau3AI GATC 4 cut(s) 91, 322, 385, 1084
Sau96I GGNCC 1 cut(s) 241
SchI GAGTC 3 cut(s) 967, 1033, 1320
SfaNI GCATC 4 cut(s) 83, 145, 743, 1146
SfcI CTRYAG 1 cut(s) 374
Sfr303I CCGCGG 1 cut(s) 783
SgrBI CCGCGG 1 cut(s) 783
SinI GGWCC 1 cut(s) 241
SmiMI CAYNNNNRTG 1 cut(s) 1398
SsiI CCGC 3 cut(s) 558, 780, 782
SspI AATATT 1 cut(s) 792
SspMI CTAG 2 cut(s) 212, 311
StyI CCWWGG 2 cut(s) 254, 1088
TaaI ACNGT 7 cut(s) 163, 245, 278, 304, 580, 889, 1234
TaiI ACGT 2 cut(s) 351, 623
TaqI TCGA 1 cut(s) 1141
TatI WGTACW 2 cut(s) 457, 478
TauI GCSGC 1 cut(s) 785
TfiI GAWTC 5 cut(s) 827, 852, 921, 995, 1418
Tru1I TTAA 3 cut(s) 720, 1193, 1313
Tru9I TTAA 3 cut(s) 720, 1193, 1313
TscAI CASTG 6 cut(s) 283, 655, 1059, 1221, 1228, 1237
TseFI GTSAC 7 cut(s) 652, 754, 1036, 1057, 1216, 1228, 1234
TseI GCWGC 3 cut(s) 498, 941, 1436
Tsp45I GTSAC 7 cut(s) 652, 754, 1036, 1057, 1216, 1228, 1234
TspDTI ATGAA 9 cut(s) 132, 587, 913, 1110, 1174, 1192, 1314, 1389, 1445
TspGWI ACGGA 1 cut(s) 167
TspRI CASTG 6 cut(s) 283, 655, 1059, 1221, 1228, 1237
VpaK11BI GGWCC 1 cut(s) 241
XapI RAATTY 7 cut(s) 37, 50, 179, 905, 978, 1199, 1301
XmnI GAANNNNTTC 1 cut(s) 1203
XspI CTAG 2 cut(s) 212, 311
Zsp2I ATGCAT 1 cut(s) 736
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.