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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 207 |
| AarI | CACCTGC | 1 cut(s) | 577 |
| Acc36I | ACCTGC | 1 cut(s) | 577 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 283 |
| AcsI | RAATTY | 2 cut(s) | 237, 399 |
| AfaI | GTAC | 1 cut(s) | 56 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 283 |
| AgsI | TTSAA | 1 cut(s) | 554 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 436, 468 |
| AluBI | AGCT | 3 cut(s) | 216, 560, 618 |
| AluI | AGCT | 3 cut(s) | 216, 560, 618 |
| AoxI | GGCC | 1 cut(s) | 508 |
| ApeKI | GCWGC | 2 cut(s) | 14, 515 |
| ApoI | RAATTY | 2 cut(s) | 237, 399 |
| AsuHPI | GGTGA | 1 cut(s) | 371 |
| BaeGI | GKGCMC | 1 cut(s) | 420 |
| BbvI | GCAGC | 2 cut(s) | 26, 502 |
| BccI | CCATC | 3 cut(s) | 62, 154, 182 |
| BclI | TGATCA | 1 cut(s) | 460 |
| BfuAI | ACCTGC | 1 cut(s) | 577 |
| BisI | GCNGC | 2 cut(s) | 15, 516 |
| BlsI | GCNGC | 2 cut(s) | 16, 517 |
| BmcAI | AGTACT | 1 cut(s) | 56 |
| BmsI | GCATC | 1 cut(s) | 317 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 205, 235 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 283 |
| Bse3DI | GCAATG | 1 cut(s) | 15 |
| BseGI | GGATG | 3 cut(s) | 193, 463, 620 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 283 |
| BseMI | GCAATG | 1 cut(s) | 15 |
| BseMII | CTCAG | 2 cut(s) | 72, 381 |
| BseSI | GKGCMC | 1 cut(s) | 420 |
| BseXI | GCAGC | 2 cut(s) | 26, 502 |
| BshFI | GGCC | 1 cut(s) | 510 |
| BslFI | GGGAC | 1 cut(s) | 267 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 283 |
| BsmFI | GGGAC | 1 cut(s) | 267 |
| BsmI | GAATGC | 1 cut(s) | 82 |
| BsnI | GGCC | 1 cut(s) | 510 |
| Bsp1286I | GDGCHC | 1 cut(s) | 420 |
| Bsp143I | GATC | 1 cut(s) | 460 |
| BspANI | GGCC | 1 cut(s) | 510 |
| BspCNI | CTCAG | 2 cut(s) | 71, 382 |
| BspMI | ACCTGC | 1 cut(s) | 577 |
| BsrDI | GCAATG | 1 cut(s) | 15 |
| BssMI | GATC | 1 cut(s) | 460 |
| Bst4CI | ACNGT | 4 cut(s) | 54, 527, 583, 611 |
| BstC8I | GCNNGC | 1 cut(s) | 616 |
| BstDEI | CTNAG | 2 cut(s) | 58, 390 |
| BstF5I | GGATG | 3 cut(s) | 193, 463, 620 |
| BstKTI | GATC | 1 cut(s) | 463 |
| BstMBI | GATC | 1 cut(s) | 460 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 14 |
| BstSLI | GKGCMC | 1 cut(s) | 420 |
| BstV1I | GCAGC | 2 cut(s) | 26, 502 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 153 |
| BsuRI | GGCC | 1 cut(s) | 510 |
| BtsCI | GGATG | 3 cut(s) | 193, 463, 620 |
| BtsI | GCAGTG | 2 cut(s) | 205, 420 |
| BtsIMutI | CAGTG | 3 cut(s) | 205, 420, 579 |
| BveI | ACCTGC | 1 cut(s) | 577 |
| Cac8I | GCNNGC | 1 cut(s) | 616 |
| Csp6I | GTAC | 1 cut(s) | 55 |
| CviAII | CATG | 4 cut(s) | 153, 283, 512, 596 |
| CviJI | RGCY | 5 cut(s) | 145, 216, 510, 560, 618 |
| CviKI_1 | RGCY | 5 cut(s) | 145, 216, 510, 560, 618 |
| CviQI | GTAC | 1 cut(s) | 55 |
| DdeI | CTNAG | 2 cut(s) | 58, 390 |
| DpnI | GATC | 1 cut(s) | 462 |
| DpnII | GATC | 1 cut(s) | 460 |
| FaeI | CATG | 4 cut(s) | 156, 286, 515, 599 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 187, 219 |
| FaqI | GGGAC | 1 cut(s) | 267 |
| FatI | CATG | 4 cut(s) | 152, 282, 511, 595 |
| FbaI | TGATCA | 1 cut(s) | 460 |
| Fnu4HI | GCNGC | 2 cut(s) | 15, 516 |
| FokI | GGATG | 3 cut(s) | 200, 450, 607 |
| Fsp4HI | GCNGC | 2 cut(s) | 15, 516 |
| GluI | GCNGC | 2 cut(s) | 15, 516 |
| HaeIII | GGCC | 1 cut(s) | 510 |
| Hin1II | CATG | 4 cut(s) | 156, 286, 515, 599 |
| HphI | GGTGA | 1 cut(s) | 371 |
| Hpy166II | GTNNAC | 2 cut(s) | 430, 500 |
| Hpy188III | TCNNGA | 2 cut(s) | 60, 389 |
| Hpy8I | GTNNAC | 2 cut(s) | 430, 500 |
| HpyAV | CCTTC | 1 cut(s) | 244 |
| HpyCH4III | ACNGT | 4 cut(s) | 54, 527, 583, 611 |
| HpyCH4V | TGCA | 8 cut(s) | 8, 152, 263, 320, 344, 470, 606, 614 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 14 |
| HpyF3I | CTNAG | 2 cut(s) | 58, 390 |
| Hsp92II | CATG | 4 cut(s) | 156, 286, 515, 599 |
| Ksp22I | TGATCA | 1 cut(s) | 460 |
| Kzo9I | GATC | 1 cut(s) | 460 |
| LmnI | GCTCC | 3 cut(s) | 156, 213, 557 |
| Lsp1109I | GCAGC | 2 cut(s) | 26, 502 |
| LweI | GCATC | 1 cut(s) | 317 |
| MaeIII | GTNAC | 3 cut(s) | 266, 290, 299 |
| MalI | GATC | 1 cut(s) | 462 |
| MboI | GATC | 1 cut(s) | 460 |
| MhlI | GDGCHC | 1 cut(s) | 420 |
| MluCI | AATT | 5 cut(s) | 237, 399, 541, 601, 625 |
| MnlI | CCTC | 2 cut(s) | 407, 583 |
| MseI | TTAA | 1 cut(s) | 504 |
| MslI | CAYNNNNRTG | 2 cut(s) | 151, 584 |
| Mva1269I | GAATGC | 1 cut(s) | 82 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 14 |
| NdeII | GATC | 1 cut(s) | 460 |
| NlaIII | CATG | 4 cut(s) | 156, 286, 515, 599 |
| NmuCI | GTSAC | 2 cut(s) | 290, 299 |
| PaqCI | CACCTGC | 1 cut(s) | 577 |
| PctI | GAATGC | 1 cut(s) | 82 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 283 |
| PkrI | GCNGC | 2 cut(s) | 16, 517 |
| PsiI | TTATAA | 1 cut(s) | 207 |
| RsaI | GTAC | 1 cut(s) | 56 |
| RsaNI | GTAC | 1 cut(s) | 55 |
| RseI | CAYNNNNRTG | 2 cut(s) | 151, 584 |
| SaqAI | TTAA | 1 cut(s) | 504 |
| SatI | GCNGC | 2 cut(s) | 15, 516 |
| Sau3AI | GATC | 1 cut(s) | 460 |
| ScaI | AGTACT | 1 cut(s) | 56 |
| SduI | GDGCHC | 1 cut(s) | 420 |
| SetI | ASST | 4 cut(s) | 218, 562, 571, 620 |
| SfaNI | GCATC | 1 cut(s) | 317 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 151, 584 |
| Sse9I | AATT | 5 cut(s) | 237, 399, 541, 601, 625 |
| TaaI | ACNGT | 4 cut(s) | 54, 527, 583, 611 |
| TaqI | TCGA | 1 cut(s) | 325 |
| TasI | AATT | 5 cut(s) | 237, 399, 541, 601, 625 |
| TatI | WGTACW | 1 cut(s) | 54 |
| Tru1I | TTAA | 1 cut(s) | 504 |
| Tru9I | TTAA | 1 cut(s) | 504 |
| TscAI | CASTG | 3 cut(s) | 205, 427, 586 |
| TseFI | GTSAC | 2 cut(s) | 290, 299 |
| TseI | GCWGC | 2 cut(s) | 14, 515 |
| Tsp45I | GTSAC | 2 cut(s) | 290, 299 |
| TspDTI | ATGAA | 2 cut(s) | 75, 618 |
| TspRI | CASTG | 3 cut(s) | 205, 427, 586 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 283 |
| XapI | RAATTY | 2 cut(s) | 237, 399 |
| ZrmI | AGTACT | 1 cut(s) | 56 |