FvH4_3g22340

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001357
Physical Location & Seq
Forward (+)
15359494 .. 15362323
2830 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g22340.t1

Sequence Viewer

Length: 1503 bp
ATGGCTACAACTCTTCTTGTTTTTCTGCTGAAGGATTTAATGGAGGTTACAGAAGTCGGAGTACCAACAATGTCAGAAACTACAAAGGGAATCATCGAGGGAAGCCATATGGAAGGAAATCCCAATAATGATCAGGGAAAAATCTATGAACAGAAATCTGATACTTGGAATGGAAATAACAAAAGTAGAGGAAGTGGTTCTTTTCACTACTGGAATGATGGTGAAGGCTATAGAAATAACTTTGGGTTATCTTCAAAGTGTCAAATTTGTAAGAAGAAAGGTCACATTGCTATAAATTGCTGGTACAGAAATGACATTCCTCTTAGTTATCCATCTAACTCTATGATCACTTGTCAAATATGTGGACTGAAAGGACATGCTGCTTTGGACTGCCGTTACAGATCAACCTGTACATTTCAAGGAGCCGAACCACCTTCTACTCCTACTGTGATGACTGCACATATTACCAACAGAGCTAGTTTTTCTGGTTTATCATCTCTAGTACCATTTGTTGCTGTCCCTGCCTCAGTTGAGAGGGGGAATACTAGTGGTAATGTTGAAAATCATGGTGGGGGTGGTGATGTTGTAGTTGGTGGCAGTGAAAATAATTCTGGAGGAGGTAATGGTGATATGGGAAATAATGTTGGTGGTGGTAATCATGGTGGGGGTGGTAATGTTTTATCTTGTGAACTTCAATCTGCTCAAGATCAAGCATCTAAACAACATGTCGTGCCAACCAGAATCAAAGATGAATTTGACAAAAAGATGTCGTGTCAAGATTTGTGTGCTTTCTCCTGCGTTGCTCCACAAAAGCTGAAGCAAGAGATTACTTACAGACACAATGACGTGTTCGTCTTTCCAGAAAAATATGCAAAGGAGTTAGTTCATGATGCTGGTATAGATACATCAAGATCTTGCTCAGTTCCTTGCGAATCACATCTTCAAGTGCTCAAGATTGAAGGTAGTCATGGTACTCTTCTCAGTGATCTTTCTTTGTTAAGACGCATTGTTGATGCTCTCCAGTACTTGAGTTTTTCAAGACTCGATATCCTCTTTGTTGCAAATCTTGTGTGCCAGTTCTTGACAGCAGAAAGGTCATGTAGTTGCAGGGAAGGTAATGTAGAAGCAGTGACCTGCAGCGGCGCCACTGATGCACCAGCATTAGAAGAAGAAGCTGTAGGACTGTCTATGGGAATTCAAGGAACAGAAGTTGCCAAAGGAAGCTCAGCTATTATAATCACAGATGATGATAACTTTGCTTCAGTGCGGCTTCATGAATATGATTATGGACACGCTGCTAGCTCTGGCACTTACCAAGGACAAACCCACAAAAGAACTGATGGAAAAGCAACCGGTATATTCAATGAGTTTAATGCAAGGCAGCTTGAGAAGAAGGATATATTTAGGAATATCCACGCCGATAAATTGTTTAAGGGGATAATTGCTGTGGCAGTTATTCTGCAGATTGTGGCAGTTTATTTTCAAGCAAATTTGGAGATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

501

Amino Acids

54.02

Weight (kDa)

5.9

Isoelectric Point (pI)

39.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1235
AasI GACNNNNNNGTC 1 cut(s) 851
Acc36I ACCTGC 1 cut(s) 1142
AccB1I GGYRCC 1 cut(s) 1142
AciI CCGC 2 cut(s) 1140, 1267
AcsI RAATTY 4 cut(s) 264, 752, 1194, 1489
AcuI CTGAAG 3 cut(s) 50, 836, 1245
AcyI GRCGYC 1 cut(s) 1143
AfaI GTAC 6 cut(s) 63, 305, 412, 504, 973, 1025
AflIII ACRYGT 2 cut(s) 724, 846
AgeI ACCGGT 1 cut(s) 1352
AhlI ACTAGT 1 cut(s) 545
AjiI CACGTC 1 cut(s) 847
AluBI AGCT 7 cut(s) 476, 814, 1175, 1224, 1229, 1302, 1384
AluI AGCT 7 cut(s) 476, 814, 1175, 1224, 1229, 1302, 1384
Alw21I GWGCWC 1 cut(s) 951
ApeKI GCWGC 4 cut(s) 380, 1137, 1295, 1381
ApoI RAATTY 4 cut(s) 264, 752, 1194, 1489
AsiGI ACCGGT 1 cut(s) 1352
Asp700I GAANNNNTTC 1 cut(s) 196
AspLEI GCGC 1 cut(s) 1145
AsuHPI GGTGA 3 cut(s) 233, 590, 638
AsuNHI GCTAGC 1 cut(s) 1298
BanI GGYRCC 1 cut(s) 1142
BarI GAAGNNNNNNTAC 2 cut(s) 45, 77
Bbv12I GWGCWC 1 cut(s) 951
BbvI GCAGC 4 cut(s) 367, 1149, 1282, 1393
BccI CCATC 3 cut(s) 212, 340, 1334
BceAI ACGGC 1 cut(s) 378
BclI TGATCA 2 cut(s) 130, 345
BcuI ACTAGT 1 cut(s) 545
BfaI CTAG 4 cut(s) 477, 500, 546, 1299
BfmI CTRYAG 4 cut(s) 229, 1135, 1176, 1460
BfoI RGCGCY 1 cut(s) 1146
BfuAI ACCTGC 1 cut(s) 1142
BglII AGATCT 1 cut(s) 911
BisI GCNGC 6 cut(s) 381, 1138, 1141, 1268, 1296, 1382
BlpI GCTNAGC 1 cut(s) 1225
BlsI GCNGC 6 cut(s) 382, 1139, 1142, 1269, 1297, 1383
BmcAI AGTACT 1 cut(s) 1025
BmgBI CACGTC 1 cut(s) 847
BmiI GGNNCC 2 cut(s) 424, 1144
BmsI GCATC 4 cut(s) 722, 880, 1003, 1141
BmtI GCTAGC 1 cut(s) 1302
BpmI CTGGAG 2 cut(s) 633, 1004
Bpu1102I GCTNAGC 1 cut(s) 1225
BpuEI CTTGAG 4 cut(s) 687, 935, 1048, 1406
BsaHI GRCGYC 1 cut(s) 1143
BsaJI CCNNGG 1 cut(s) 1315
BsaWI WCCGGW 1 cut(s) 1352
Bse118I RCCGGY 1 cut(s) 1352
Bse1I ACTGG 3 cut(s) 215, 1021, 1075
Bse3DI GCAATG 1 cut(s) 285
BseDI CCNNGG 1 cut(s) 1315
BseMI GCAATG 1 cut(s) 285
BseMII CTCAG 4 cut(s) 540, 933, 994, 1239
BseNI ACTGG 3 cut(s) 215, 1021, 1075
BseRI GAGGAG 1 cut(s) 630
BseXI GCAGC 4 cut(s) 367, 1149, 1282, 1393
BsgI GTGCAG 1 cut(s) 441
BshNI GGYRCC 1 cut(s) 1142
BshTI ACCGGT 1 cut(s) 1352
BsiHKAI GWGCWC 1 cut(s) 951
BsiSI CCGG 1 cut(s) 1353
BslFI GGGAC 1 cut(s) 503
BsmFI GGGAC 1 cut(s) 503
Bsp1286I GDGCHC 1 cut(s) 951
Bsp1407I TGTACA 1 cut(s) 410
Bsp143I GATC 6 cut(s) 130, 345, 401, 706, 911, 985
Bsp1720I GCTNAGC 1 cut(s) 1225
BspACI CCGC 2 cut(s) 1140, 1267
BspCNI CTCAG 4 cut(s) 539, 932, 993, 1238
BspHI TCATGA 2 cut(s) 886, 1273
BspLI GGNNCC 2 cut(s) 424, 1144
BspMAI CTGCAG 2 cut(s) 1139, 1464
BspMI ACCTGC 1 cut(s) 1142
BspOI GCTAGC 1 cut(s) 1302
BspT107I GGYRCC 1 cut(s) 1142
BsrDI GCAATG 1 cut(s) 285
BsrFI RCCGGY 1 cut(s) 1352
BsrGI TGTACA 1 cut(s) 410
BsrI ACTGG 3 cut(s) 215, 1021, 1075
BssAI RCCGGY 1 cut(s) 1352
BssECI CCNNGG 1 cut(s) 1315
BssMI GATC 6 cut(s) 130, 345, 401, 706, 911, 985
BssNI GRCGYC 1 cut(s) 1143
BssT1I CCWWGG 1 cut(s) 1315
Bst4CI ACNGT 2 cut(s) 448, 1185
Bst6I CTCTTC 2 cut(s) 18, 981
BstACI GRCGYC 1 cut(s) 1143
BstAUI TGTACA 1 cut(s) 410
BstC8I GCNNGC 1 cut(s) 1300
BstDEI CTNAG 5 cut(s) 323, 526, 919, 980, 1225
BstH2I RGCGCY 1 cut(s) 1146
BstHHI GCGC 1 cut(s) 1145
BstKTI GATC 6 cut(s) 133, 348, 404, 709, 914, 988
BstMBI GATC 6 cut(s) 130, 345, 401, 706, 911, 985
BstMWI GCNNNNNNNGC 2 cut(s) 521, 1151
BstNSI RCATGY 2 cut(s) 380, 728
BstSFI CTRYAG 4 cut(s) 229, 1135, 1176, 1460
BstV1I GCAGC 4 cut(s) 367, 1149, 1282, 1393
BstX2I RGATCY 1 cut(s) 911
BstYI RGATCY 1 cut(s) 911
BtrI CACGTC 1 cut(s) 847
BtsI GCAGTG 2 cut(s) 604, 1134
BtsIMutI CAGTG 5 cut(s) 604, 988, 1134, 1146, 1269
BveI ACCTGC 1 cut(s) 1142
Cac8I GCNNGC 1 cut(s) 1300
CciI TCATGA 2 cut(s) 886, 1273
CfoI GCGC 1 cut(s) 1145
Cfr10I RCCGGY 1 cut(s) 1352
CseI GACGC 1 cut(s) 1011
Csp6I GTAC 6 cut(s) 62, 304, 411, 503, 972, 1024
CspAI ACCGGT 1 cut(s) 1352
CviAII CATG 8 cut(s) 377, 566, 659, 725, 887, 968, 1098, 1274
CviQI GTAC 6 cut(s) 62, 304, 411, 503, 972, 1024
DdeI CTNAG 5 cut(s) 323, 526, 919, 980, 1225
DinI GGCGCC 1 cut(s) 1144
DpnI GATC 6 cut(s) 132, 347, 403, 708, 913, 987
DpnII GATC 6 cut(s) 130, 345, 401, 706, 911, 985
DrdI GACNNNNNNGTC 1 cut(s) 851
DseDI GACNNNNNNGTC 1 cut(s) 851
Eam1104I CTCTTC 2 cut(s) 18, 981
EarI CTCTTC 2 cut(s) 18, 981
Eco130I CCWWGG 1 cut(s) 1315
Eco32I GATATC 1 cut(s) 1048
Eco57I CTGAAG 3 cut(s) 50, 836, 1245
EcoRI GAATTC 1 cut(s) 1194
EcoRV GATATC 1 cut(s) 1048
EcoT14I CCWWGG 1 cut(s) 1315
EgeI GGCGCC 1 cut(s) 1144
EheI GGCGCC 1 cut(s) 1144
ErhI CCWWGG 1 cut(s) 1315
FaeI CATG 8 cut(s) 380, 569, 662, 728, 890, 971, 1101, 1277
FalI AAGNNNNNCTT 2 cut(s) 184, 216
FaqI GGGAC 1 cut(s) 503
FatI CATG 8 cut(s) 376, 565, 658, 724, 886, 967, 1097, 1273
FauNDI CATATG 1 cut(s) 108
FbaI TGATCA 2 cut(s) 130, 345
Fnu4HI GCNGC 6 cut(s) 381, 1138, 1141, 1268, 1296, 1382
Fsp4HI GCNGC 6 cut(s) 381, 1138, 1141, 1268, 1296, 1382
FspBI CTAG 4 cut(s) 477, 500, 546, 1299
GlaI GCGC 1 cut(s) 1144
GluI GCNGC 6 cut(s) 381, 1138, 1141, 1268, 1296, 1382
GsuI CTGGAG 2 cut(s) 633, 1004
HaeII RGCGCY 1 cut(s) 1146
HapII CCGG 1 cut(s) 1353
HgaI GACGC 1 cut(s) 1011
HhaI GCGC 1 cut(s) 1145
Hin1I GRCGYC 1 cut(s) 1143
Hin1II CATG 8 cut(s) 380, 569, 662, 728, 890, 971, 1101, 1277
Hin6I GCGC 1 cut(s) 1143
HinP1I GCGC 1 cut(s) 1143
HinfI GANTC 4 cut(s) 90, 741, 932, 1041
HpaII CCGG 1 cut(s) 1353
HphI GGTGA 3 cut(s) 233, 590, 638
Hpy166II GTNNAC 2 cut(s) 365, 689
Hpy188I TCNGA 3 cut(s) 59, 76, 160
Hpy8I GTNNAC 2 cut(s) 365, 689
HpyAV CCTTC 7 cut(s) 25, 107, 218, 444, 953, 1106, 1387
HpyCH4III ACNGT 2 cut(s) 448, 1185
HpyCH4IV ACGT 1 cut(s) 846
HpyCH4V TGCA 8 cut(s) 458, 872, 1061, 1107, 1137, 1154, 1376, 1462
HpyF10VI GCNNNNNNNGC 2 cut(s) 521, 1151
HpyF3I CTNAG 5 cut(s) 323, 526, 919, 980, 1225
HpySE526I ACGT 1 cut(s) 846
Hsp92I GRCGYC 1 cut(s) 1143
Hsp92II CATG 8 cut(s) 380, 569, 662, 728, 890, 971, 1101, 1277
HspAI GCGC 1 cut(s) 1143
KasI GGCGCC 1 cut(s) 1142
Ksp22I TGATCA 2 cut(s) 130, 345
Kzo9I GATC 6 cut(s) 130, 345, 401, 706, 911, 985
LmnI GCTCC 2 cut(s) 422, 808
Lsp1109I GCAGC 4 cut(s) 367, 1149, 1282, 1393
LweI GCATC 4 cut(s) 722, 880, 1003, 1141
MaeI CTAG 4 cut(s) 477, 500, 546, 1299
MaeII ACGT 1 cut(s) 846
MaeIII GTNAC 4 cut(s) 46, 281, 395, 1129
MalI GATC 6 cut(s) 132, 347, 403, 708, 913, 987
MboI GATC 6 cut(s) 130, 345, 401, 706, 911, 985
MboII GAAGA 8 cut(s) 5, 243, 286, 932, 968, 1178, 1181, 1402
MflI RGATCY 1 cut(s) 911
MhlI GDGCHC 1 cut(s) 951
MluCI AATT 8 cut(s) 264, 295, 607, 752, 1194, 1424, 1440, 1489
Mly113I GGCGCC 1 cut(s) 1143
MlyI GAGTC 1 cut(s) 1035
MmeI TCCRAC 1 cut(s) 37
MnlI CCTC 9 cut(s) 37, 91, 182, 330, 528, 535, 608, 611, 1061
MroXI GAANNNNTTC 1 cut(s) 196
MseI TTAA 4 cut(s) 38, 998, 1371, 1431
MslI CAYNNNNRTG 1 cut(s) 1278
MspA1I CMGCKG 1 cut(s) 1140
MspI CCGG 1 cut(s) 1353
MwoI GCNNNNNNNGC 2 cut(s) 521, 1151
NarI GGCGCC 1 cut(s) 1143
NdeI CATATG 1 cut(s) 108
NdeII GATC 6 cut(s) 130, 345, 401, 706, 911, 985
NheI GCTAGC 1 cut(s) 1298
NlaIII CATG 8 cut(s) 380, 569, 662, 728, 890, 971, 1101, 1277
NlaIV GGNNCC 2 cut(s) 424, 1144
NmuCI GTSAC 2 cut(s) 281, 1129
NspI RCATGY 2 cut(s) 380, 728
PagI TCATGA 2 cut(s) 886, 1273
PciI ACATGT 1 cut(s) 724
PdmI GAANNNNTTC 1 cut(s) 196
PfeI GAWTC 3 cut(s) 90, 741, 932
PinAI ACCGGT 1 cut(s) 1352
PkrI GCNGC 6 cut(s) 382, 1139, 1142, 1269, 1297, 1383
PleI GAGTC 1 cut(s) 1035
PluTI GGCGCC 1 cut(s) 1146
PpsI GAGTC 1 cut(s) 1035
PscI ACATGT 1 cut(s) 724
PsiI TTATAA 1 cut(s) 1235
PspN4I GGNNCC 2 cut(s) 424, 1144
PstI CTGCAG 2 cut(s) 1139, 1464
PsuI RGATCY 1 cut(s) 911
RsaI GTAC 6 cut(s) 63, 305, 412, 504, 973, 1025
RsaNI GTAC 6 cut(s) 62, 304, 411, 503, 972, 1024
RseI CAYNNNNRTG 1 cut(s) 1278
SaqAI TTAA 4 cut(s) 38, 998, 1371, 1431
SatI GCNGC 6 cut(s) 381, 1138, 1141, 1268, 1296, 1382
Sau3AI GATC 6 cut(s) 130, 345, 401, 706, 911, 985
ScaI AGTACT 1 cut(s) 1025
SchI GAGTC 1 cut(s) 1035
SduI GDGCHC 1 cut(s) 951
SfaNI GCATC 4 cut(s) 722, 880, 1003, 1141
SfcI CTRYAG 4 cut(s) 229, 1135, 1176, 1460
SfoI GGCGCC 1 cut(s) 1144
SmiMI CAYNNNNRTG 1 cut(s) 1278
SmlI CTYRAG 4 cut(s) 702, 950, 1027, 1385
SmoI CTYRAG 4 cut(s) 702, 950, 1027, 1385
SpeI ACTAGT 1 cut(s) 545
Sse9I AATT 8 cut(s) 264, 295, 607, 752, 1194, 1424, 1440, 1489
SsiI CCGC 2 cut(s) 1140, 1267
SspDI GGCGCC 1 cut(s) 1142
SspMI CTAG 4 cut(s) 477, 500, 546, 1299
StyI CCWWGG 1 cut(s) 1315
TaaI ACNGT 2 cut(s) 448, 1185
TaiI ACGT 1 cut(s) 849
TaqI TCGA 2 cut(s) 96, 1044
TasI AATT 8 cut(s) 264, 295, 607, 752, 1194, 1424, 1440, 1489
TatI WGTACW 2 cut(s) 410, 1023
TauI GCSGC 2 cut(s) 1143, 1270
TfiI GAWTC 3 cut(s) 90, 741, 932
Tru1I TTAA 4 cut(s) 38, 998, 1371, 1431
Tru9I TTAA 4 cut(s) 38, 998, 1371, 1431
TscAI CASTG 5 cut(s) 604, 988, 1134, 1153, 1269
TseFI GTSAC 2 cut(s) 281, 1129
TseI GCWGC 4 cut(s) 380, 1137, 1295, 1381
Tsp45I GTSAC 2 cut(s) 281, 1129
TspDTI ATGAA 5 cut(s) 162, 765, 875, 1262, 1290
TspRI CASTG 5 cut(s) 604, 988, 1134, 1153, 1269
XapI RAATTY 4 cut(s) 264, 752, 1194, 1489
XceI RCATGY 2 cut(s) 380, 728
XmnI GAANNNNTTC 1 cut(s) 196
XspI CTAG 4 cut(s) 477, 500, 546, 1299
ZrmI AGTACT 1 cut(s) 1025
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.