MD17G1211800.v1.1
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0001357
Physical Location & Seq
Reverse (-)
25873306 .. 25875018
1713 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1211800.v1.1.491

Sequence Viewer

Length: 732 bp
ATGTATAACAATGGTGCATCCAGTTCAAATTCCTCTGCGAATGAGCCATCACGTACAGGAGGTGTCATTACAGCTATTCCTTTACCACCACAAATGTCATTATTTCCACAGTCTCAAATGGGGAAATTTGCTTCTTTTCCAGATCAGAATTCTTATGGCTCAGGTAGTGGTTTTGTTGGTAATCTGGAGCAGCATCCACAAGATACCTTGAGGCAATATCAACCTTCAACACAGTTCCATAGCATTCAACAACGGTTTAACAATGGAGGACAATCAAGGGGACATAATAATTACAGATCAAGATTCAGAGGCAAATGGTCTAATTACAGGAACAATAATGGTTGGAATGGAAACACTGAATCAAGAACTACTCTACATCCGGAATGTCAAATTTGTCAGAGAATGGGTCATACTGCTCCTAACTGTTTTTACAGAACTGCTCAGAGTACTTCCCCACATTTTAATACTGAATGTCAGATTTGTGGTAAGAGGGGACATAGTGCACTGGAATGCTATCATCGGGGAAATTACTCCTATCAACCTTCTGGACCTCCTGCCTCTCTTGCACCTATATTTCCTGGTCAACCTTCTGGGATTTCTCAATCACAAGGTTTTGTTCAACAACCTCAGAATTTTGTTGCTCATTATTCATATCAGTTGCATCCACCTACACTGCAAGGGATGAATGTTCAACAGAATCCACCAATACCTGATATTTCATATCAAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 Retrotran_gag_3 Retrotrans_gag gag_pre-integrs rve

Protein Analysis

244

Amino Acids

27.02

Weight (kDa)

9.23

Isoelectric Point (pI)

58.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 379
AcsI RAATTY 5 cut(s) 28, 125, 148, 390, 631
AfaI GTAC 2 cut(s) 55, 448
AgsI TTSAA 5 cut(s) 27, 228, 248, 620, 692
AjnI CCWGG 1 cut(s) 577
AluBI AGCT 1 cut(s) 74
AluI AGCT 1 cut(s) 74
Alw21I GWGCWC 1 cut(s) 505
Alw26I GTCTC 1 cut(s) 117
Alw44I GTGCAC 1 cut(s) 501
Aor13HI TCCGGA 1 cut(s) 379
ApaLI GTGCAC 1 cut(s) 501
ApeKI GCWGC 1 cut(s) 190
ApoI RAATTY 5 cut(s) 28, 125, 148, 390, 631
AspS9I GGNCC 1 cut(s) 548
AvaII GGWCC 1 cut(s) 548
BaeGI GKGCMC 1 cut(s) 505
Bbv12I GWGCWC 1 cut(s) 505
BbvI GCAGC 1 cut(s) 202
BccI CCATC 1 cut(s) 55
BciT130I CCWGG 1 cut(s) 579
BcoDI GTCTC 1 cut(s) 117
BisI GCNGC 1 cut(s) 191
BlsI GCNGC 1 cut(s) 192
BmcAI AGTACT 1 cut(s) 448
Bme1390I CCNGG 1 cut(s) 579
Bme18I GGWCC 1 cut(s) 548
BmgT120I GGNCC 1 cut(s) 548
BmrFI CCNGG 1 cut(s) 579
BmsI GCATC 3 cut(s) 26, 202, 670
BpmI CTGGAG 1 cut(s) 206
Bpu10I CCTNAGC 1 cut(s) 160
BpuEI CTTGAG 1 cut(s) 229
BsaAI YACGTR 1 cut(s) 53
BsaWI WCCGGW 1 cut(s) 379
Bse1I ACTGG 2 cut(s) 21, 510
BseAI TCCGGA 1 cut(s) 379
BseBI CCWGG 1 cut(s) 579
BseGI GGATG 5 cut(s) 17, 193, 376, 661, 687
BseMII CTCAG 3 cut(s) 174, 455, 641
BseNI ACTGG 2 cut(s) 21, 510
BseSI GKGCMC 1 cut(s) 505
BseXI GCAGC 1 cut(s) 202
BsiHKAI GWGCWC 1 cut(s) 505
BsiSI CCGG 1 cut(s) 380
BslFI GGGAC 2 cut(s) 294, 507
BsmAI GTCTC 1 cut(s) 117
BsmFI GGGAC 2 cut(s) 294, 507
BsmI GAATGC 2 cut(s) 243, 515
Bsp1286I GDGCHC 1 cut(s) 505
Bsp13I TCCGGA 1 cut(s) 379
Bsp143I GATC 2 cut(s) 142, 296
BspCNI CTCAG 3 cut(s) 173, 454, 640
BspEI TCCGGA 1 cut(s) 379
BsrI ACTGG 2 cut(s) 21, 510
BssMI GATC 2 cut(s) 142, 296
Bst2UI CCWGG 1 cut(s) 579
Bst4CI ACNGT 4 cut(s) 111, 234, 255, 425
BstBAI YACGTR 1 cut(s) 53
BstDEI CTNAG 3 cut(s) 160, 441, 627
BstF5I GGATG 5 cut(s) 17, 193, 376, 661, 687
BstKTI GATC 2 cut(s) 145, 299
BstMAI GTCTC 1 cut(s) 117
BstMBI GATC 2 cut(s) 142, 296
BstMWI GCNNNNNNNGC 1 cut(s) 563
BstNI CCWGG 1 cut(s) 579
BstSCI CCNGG 1 cut(s) 577
BstSLI GKGCMC 1 cut(s) 505
BstV1I GCAGC 1 cut(s) 202
BtsCI GGATG 5 cut(s) 17, 193, 376, 661, 687
BtsI GCAGTG 1 cut(s) 671
BtsIMutI CAGTG 3 cut(s) 354, 503, 671
Cfr13I GGNCC 1 cut(s) 548
Csp6I GTAC 2 cut(s) 54, 447
CviJI RGCY 3 cut(s) 46, 74, 159
CviKI_1 RGCY 3 cut(s) 46, 74, 159
CviQI GTAC 2 cut(s) 54, 447
DdeI CTNAG 3 cut(s) 160, 441, 627
DpnI GATC 2 cut(s) 144, 298
DpnII GATC 2 cut(s) 142, 296
Eco47I GGWCC 1 cut(s) 548
EcoRI GAATTC 1 cut(s) 148
EcoRII CCWGG 1 cut(s) 577
FaqI GGGAC 2 cut(s) 294, 507
Fnu4HI GCNGC 1 cut(s) 191
FokI GGATG 5 cut(s) 4, 180, 363, 648, 694
Fsp4HI GCNGC 1 cut(s) 191
GluI GCNGC 1 cut(s) 191
GsuI CTGGAG 1 cut(s) 206
HapII CCGG 1 cut(s) 380
HincII GTYRAC 1 cut(s) 584
HindII GTYRAC 1 cut(s) 584
HinfI GANTC 3 cut(s) 303, 359, 697
HpaII CCGG 1 cut(s) 380
Hpy166II GTNNAC 2 cut(s) 503, 584
Hpy188I TCNGA 6 cut(s) 147, 308, 399, 444, 477, 630
Hpy188III TCNNGA 6 cut(s) 140, 185, 300, 363, 380, 546
Hpy8I GTNNAC 2 cut(s) 503, 584
HpyAV CCTTC 3 cut(s) 234, 552, 597
HpyCH4III ACNGT 4 cut(s) 111, 234, 255, 425
HpyCH4IV ACGT 1 cut(s) 52
HpyCH4V TGCA 5 cut(s) 17, 503, 566, 661, 676
HpyF10VI GCNNNNNNNGC 1 cut(s) 563
HpyF3I CTNAG 3 cut(s) 160, 441, 627
HpySE526I ACGT 1 cut(s) 52
Kpn2I TCCGGA 1 cut(s) 379
Kzo9I GATC 2 cut(s) 142, 296
LmnI GCTCC 2 cut(s) 187, 421
Lsp1109I GCAGC 1 cut(s) 202
LweI GCATC 3 cut(s) 26, 202, 670
MaeII ACGT 1 cut(s) 52
MalI GATC 2 cut(s) 144, 298
MboI GATC 2 cut(s) 142, 296
MhlI GDGCHC 1 cut(s) 505
MluCI AATT 8 cut(s) 28, 125, 148, 289, 322, 390, 526, 631
MmeI TCCRAC 1 cut(s) 323
MnlI CCTC 9 cut(s) 43, 53, 204, 260, 302, 483, 561, 568, 636
MroI TCCGGA 1 cut(s) 379
MseI TTAA 2 cut(s) 258, 462
MslI CAYNNNNRTG 1 cut(s) 508
MspI CCGG 1 cut(s) 380
MspR9I CCNGG 1 cut(s) 579
Mva1269I GAATGC 2 cut(s) 243, 515
MvaI CCWGG 1 cut(s) 579
MwoI GCNNNNNNNGC 1 cut(s) 563
NdeII GATC 2 cut(s) 142, 296
PctI GAATGC 2 cut(s) 243, 515
PfeI GAWTC 3 cut(s) 303, 359, 697
PkrI GCNGC 1 cut(s) 192
Ppu21I YACGTR 1 cut(s) 53
Psp6I CCWGG 1 cut(s) 577
PspGI CCWGG 1 cut(s) 577
PspPI GGNCC 1 cut(s) 548
RsaI GTAC 2 cut(s) 55, 448
RsaNI GTAC 2 cut(s) 54, 447
RseI CAYNNNNRTG 1 cut(s) 508
SaqAI TTAA 2 cut(s) 258, 462
SatI GCNGC 1 cut(s) 191
Sau3AI GATC 2 cut(s) 142, 296
Sau96I GGNCC 1 cut(s) 548
ScaI AGTACT 1 cut(s) 448
ScrFI CCNGG 1 cut(s) 579
SduI GDGCHC 1 cut(s) 505
SfaNI GCATC 3 cut(s) 26, 202, 670
SinI GGWCC 1 cut(s) 548
SmiMI CAYNNNNRTG 1 cut(s) 508
SmlI CTYRAG 1 cut(s) 208
SmoI CTYRAG 1 cut(s) 208
Sse9I AATT 8 cut(s) 28, 125, 148, 289, 322, 390, 526, 631
StyD4I CCNGG 1 cut(s) 577
TaaI ACNGT 4 cut(s) 111, 234, 255, 425
TaiI ACGT 1 cut(s) 55
TasI AATT 8 cut(s) 28, 125, 148, 289, 322, 390, 526, 631
TatI WGTACW 1 cut(s) 446
TfiI GAWTC 3 cut(s) 303, 359, 697
Tru1I TTAA 2 cut(s) 258, 462
Tru9I TTAA 2 cut(s) 258, 462
TscAI CASTG 3 cut(s) 361, 510, 678
TseI GCWGC 1 cut(s) 190
TspDTI ATGAA 3 cut(s) 639, 698, 708
TspRI CASTG 3 cut(s) 361, 510, 678
VneI GTGCAC 1 cut(s) 501
VpaK11BI GGWCC 1 cut(s) 548
XapI RAATTY 5 cut(s) 28, 125, 148, 390, 631
ZrmI AGTACT 1 cut(s) 448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.