Catalytic subunit of pyrophosphate--fructose 6-phosphate 1-phosphotransferase. Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis. Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| PFK | PF00365 | 235 - 340 | 1.1e-12 | Phosphofructokinase |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 832 |
| AccI | GTMKAC | 1 cut(s) | 1420 |
| AccIII | TCCGGA | 1 cut(s) | 523 |
| AclWI | GGATC | 2 cut(s) | 534, 1187 |
| AcsI | RAATTY | 5 cut(s) | 7, 128, 762, 1095, 1219 |
| AcuI | CTGAAG | 3 cut(s) | 27, 359, 1059 |
| AcvI | CACGTG | 1 cut(s) | 1467 |
| AdeI | CACNNNGTG | 1 cut(s) | 751 |
| AfaI | GTAC | 5 cut(s) | 39, 496, 1068, 1324, 1475 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 491 |
| AflIII | ACRYGT | 1 cut(s) | 1466 |
| AgsI | TTSAA | 7 cut(s) | 12, 389, 703, 769, 932, 1090, 1271 |
| AhlI | ACTAGT | 2 cut(s) | 497, 1298 |
| AjnI | CCWGG | 2 cut(s) | 97, 736 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 965, 997 |
| AleI | CACNNNNGTG | 1 cut(s) | 588 |
| Alw21I | GWGCWC | 1 cut(s) | 800 |
| Alw26I | GTCTC | 3 cut(s) | 915, 956, 1161 |
| AlwI | GGATC | 2 cut(s) | 534, 1187 |
| Aor13HI | TCCGGA | 1 cut(s) | 523 |
| AoxI | GGCC | 2 cut(s) | 250, 727 |
| ApeKI | GCWGC | 3 cut(s) | 350, 416, 685 |
| ApoI | RAATTY | 5 cut(s) | 7, 128, 762, 1095, 1219 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 319, 351 |
| AspS9I | GGNCC | 1 cut(s) | 821 |
| AsuHPI | GGTGA | 2 cut(s) | 205, 212 |
| AsuNHI | GCTAGC | 1 cut(s) | 154 |
| AvaII | GGWCC | 1 cut(s) | 821 |
| AxyI | CCTNAGG | 1 cut(s) | 1062 |
| BbrPI | CACGTG | 1 cut(s) | 1467 |
| BbsI | GAAGAC | 1 cut(s) | 1268 |
| Bbv12I | GWGCWC | 1 cut(s) | 800 |
| BbvI | GCAGC | 3 cut(s) | 362, 403, 672 |
| BccI | CCATC | 5 cut(s) | 372, 605, 980, 1112, 1118 |
| BciT130I | CCWGG | 2 cut(s) | 99, 738 |
| BclI | TGATCA | 2 cut(s) | 315, 640 |
| BcoDI | GTCTC | 3 cut(s) | 915, 956, 1161 |
| BcuI | ACTAGT | 2 cut(s) | 497, 1298 |
| BfaI | CTAG | 7 cut(s) | 155, 447, 456, 498, 675, 1299, 1452 |
| BfmI | CTRYAG | 3 cut(s) | 238, 274, 1160 |
| BfuAI | ACCTGC | 1 cut(s) | 832 |
| BisI | GCNGC | 3 cut(s) | 351, 417, 686 |
| BlsI | GCNGC | 3 cut(s) | 352, 418, 687 |
| Bme1390I | CCNGG | 2 cut(s) | 99, 738 |
| Bme18I | GGWCC | 1 cut(s) | 821 |
| BmgT120I | GGNCC | 1 cut(s) | 821 |
| BmrFI | CCNGG | 2 cut(s) | 99, 738 |
| BmsI | GCATC | 1 cut(s) | 640 |
| BmtI | GCTAGC | 1 cut(s) | 158 |
| BpiI | GAAGAC | 1 cut(s) | 1268 |
| BpmI | CTGGAG | 4 cut(s) | 744, 759, 921, 1391 |
| BsaAI | YACGTR | 1 cut(s) | 1467 |
| BsaJI | CCNNGG | 2 cut(s) | 27, 354 |
| BsaWI | WCCGGW | 2 cut(s) | 523, 862 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 487, 517 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 491 |
| Bse1I | ACTGG | 1 cut(s) | 1324 |
| Bse21I | CCTNAGG | 1 cut(s) | 1062 |
| Bse3DI | GCAATG | 2 cut(s) | 426, 1478 |
| BseAI | TCCGGA | 1 cut(s) | 523 |
| BseBI | CCWGG | 2 cut(s) | 99, 738 |
| BseDI | CCNNGG | 2 cut(s) | 27, 354 |
| BseGI | GGATG | 2 cut(s) | 285, 991 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 491 |
| BseMI | GCAATG | 2 cut(s) | 426, 1478 |
| BseMII | CTCAG | 3 cut(s) | 1053, 1388, 1470 |
| BseNI | ACTGG | 1 cut(s) | 1324 |
| BseRI | GAGGAG | 2 cut(s) | 495, 1020 |
| BseXI | GCAGC | 3 cut(s) | 362, 403, 672 |
| BsgI | GTGCAG | 2 cut(s) | 456, 699 |
| BshFI | GGCC | 2 cut(s) | 252, 729 |
| BsiHKAI | GWGCWC | 1 cut(s) | 800 |
| BsiSI | CCGG | 2 cut(s) | 524, 863 |
| BslFI | GGGAC | 1 cut(s) | 357 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 491 |
| BsmAI | GTCTC | 3 cut(s) | 915, 956, 1161 |
| BsmFI | GGGAC | 1 cut(s) | 357 |
| BsnI | GGCC | 2 cut(s) | 252, 729 |
| Bsp1286I | GDGCHC | 1 cut(s) | 800 |
| Bsp13I | TCCGGA | 1 cut(s) | 523 |
| Bsp143I | GATC | 7 cut(s) | 315, 526, 619, 640, 781, 1192, 1368 |
| BspANI | GGCC | 2 cut(s) | 252, 729 |
| BspCNI | CTCAG | 3 cut(s) | 1054, 1389, 1469 |
| BspEI | TCCGGA | 1 cut(s) | 523 |
| BspHI | TCATGA | 1 cut(s) | 834 |
| BspMI | ACCTGC | 1 cut(s) | 832 |
| BspOI | GCTAGC | 1 cut(s) | 158 |
| BspPI | GGATC | 2 cut(s) | 534, 1187 |
| BsrDI | GCAATG | 2 cut(s) | 426, 1478 |
| BsrI | ACTGG | 1 cut(s) | 1324 |
| BssECI | CCNNGG | 2 cut(s) | 27, 354 |
| BssMI | GATC | 7 cut(s) | 315, 526, 619, 640, 781, 1192, 1368 |
| BssNAI | GTATAC | 1 cut(s) | 1421 |
| BssT1I | CCWWGG | 2 cut(s) | 27, 354 |
| Bst1107I | GTATAC | 1 cut(s) | 1421 |
| Bst2UI | CCWGG | 2 cut(s) | 99, 738 |
| Bst4CI | ACNGT | 5 cut(s) | 21, 211, 427, 657, 1022 |
| Bst6I | CTCTTC | 1 cut(s) | 913 |
| BstBAI | YACGTR | 1 cut(s) | 1467 |
| BstC8I | GCNNGC | 2 cut(s) | 156, 954 |
| BstDEI | CTNAG | 5 cut(s) | 224, 1062, 1397, 1456, 1491 |
| BstF5I | GGATG | 2 cut(s) | 285, 991 |
| BstKTI | GATC | 7 cut(s) | 318, 529, 622, 643, 784, 1195, 1371 |
| BstMAI | GTCTC | 3 cut(s) | 915, 956, 1161 |
| BstMBI | GATC | 7 cut(s) | 315, 526, 619, 640, 781, 1192, 1368 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 74, 949 |
| BstNI | CCWGG | 2 cut(s) | 99, 738 |
| BstNSI | RCATGY | 1 cut(s) | 350 |
| BstSCI | CCNGG | 2 cut(s) | 97, 736 |
| BstSFI | CTRYAG | 3 cut(s) | 238, 274, 1160 |
| BstV1I | GCAGC | 3 cut(s) | 362, 403, 672 |
| BstV2I | GAAGAC | 1 cut(s) | 1268 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 737 |
| BstZ17I | GTATAC | 1 cut(s) | 1421 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1062 |
| BsuRI | GGCC | 2 cut(s) | 252, 729 |
| BtsCI | GGATG | 2 cut(s) | 285, 991 |
| BtsI | GCAGTG | 1 cut(s) | 255 |
| BtsIMutI | CAGTG | 2 cut(s) | 216, 255 |
| BveI | ACCTGC | 1 cut(s) | 832 |
| Cac8I | GCNNGC | 2 cut(s) | 156, 954 |
| CciI | TCATGA | 1 cut(s) | 834 |
| Cfr13I | GGNCC | 1 cut(s) | 821 |
| Csp6I | GTAC | 5 cut(s) | 38, 495, 1067, 1323, 1474 |
| CviAII | CATG | 8 cut(s) | 52, 347, 731, 835, 959, 1106, 1225, 1339 |
| CviQI | GTAC | 5 cut(s) | 38, 495, 1067, 1323, 1474 |
| DdeI | CTNAG | 5 cut(s) | 224, 1062, 1397, 1456, 1491 |
| DpnI | GATC | 7 cut(s) | 317, 528, 621, 642, 783, 1194, 1370 |
| DpnII | GATC | 7 cut(s) | 315, 526, 619, 640, 781, 1192, 1368 |
| DraIII | CACNNNGTG | 1 cut(s) | 751 |
| Eam1104I | CTCTTC | 1 cut(s) | 913 |
| EarI | CTCTTC | 1 cut(s) | 913 |
| Eco130I | CCWWGG | 2 cut(s) | 27, 354 |
| Eco32I | GATATC | 1 cut(s) | 218 |
| Eco47I | GGWCC | 1 cut(s) | 821 |
| Eco57I | CTGAAG | 3 cut(s) | 27, 359, 1059 |
| Eco72I | CACGTG | 1 cut(s) | 1467 |
| Eco81I | CCTNAGG | 1 cut(s) | 1062 |
| EcoRII | CCWGG | 2 cut(s) | 97, 736 |
| EcoRV | GATATC | 1 cut(s) | 218 |
| EcoT14I | CCWWGG | 2 cut(s) | 27, 354 |
| EcoT22I | ATGCAT | 1 cut(s) | 385 |
| ErhI | CCWWGG | 2 cut(s) | 27, 354 |
| FaeI | CATG | 8 cut(s) | 55, 350, 734, 838, 962, 1109, 1228, 1342 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 528, 560 |
| FaqI | GGGAC | 1 cut(s) | 357 |
| FatI | CATG | 8 cut(s) | 51, 346, 730, 834, 958, 1105, 1224, 1338 |
| FauNDI | CATATG | 2 cut(s) | 487, 1428 |
| FbaI | TGATCA | 2 cut(s) | 315, 640 |
| FblI | GTMKAC | 1 cut(s) | 1420 |
| Fnu4HI | GCNGC | 3 cut(s) | 351, 417, 686 |
| FokI | GGATG | 2 cut(s) | 272, 998 |
| Fsp4HI | GCNGC | 3 cut(s) | 351, 417, 686 |
| FspBI | CTAG | 7 cut(s) | 155, 447, 456, 498, 675, 1299, 1452 |
| GluI | GCNGC | 3 cut(s) | 351, 417, 686 |
| GsuI | CTGGAG | 4 cut(s) | 744, 759, 921, 1391 |
| HaeIII | GGCC | 2 cut(s) | 252, 729 |
| HapII | CCGG | 2 cut(s) | 524, 863 |
| Hin1II | CATG | 8 cut(s) | 55, 350, 734, 838, 962, 1109, 1228, 1342 |
| HincII | GTYRAC | 1 cut(s) | 283 |
| HindII | GTYRAC | 1 cut(s) | 283 |
| HindIII | AAGCTT | 1 cut(s) | 974 |
| HinfI | GANTC | 4 cut(s) | 48, 171, 671, 1013 |
| HpaII | CCGG | 2 cut(s) | 524, 863 |
| HphI | GGTGA | 2 cut(s) | 205, 212 |
| Hpy166II | GTNNAC | 5 cut(s) | 283, 821, 1184, 1421, 1476 |
| Hpy188I | TCNGA | 5 cut(s) | 339, 624, 815, 1209, 1398 |
| Hpy8I | GTNNAC | 5 cut(s) | 283, 821, 1184, 1421, 1476 |
| HpyAV | CCTTC | 8 cut(s) | 51, 135, 334, 414, 522, 1034, 1400, 1434 |
| HpyCH4III | ACNGT | 5 cut(s) | 21, 211, 427, 657, 1022 |
| HpyCH4IV | ACGT | 2 cut(s) | 849, 1466 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 74, 949 |
| HpyF3I | CTNAG | 5 cut(s) | 224, 1062, 1397, 1456, 1491 |
| HpySE526I | ACGT | 2 cut(s) | 849, 1466 |
| Hsp92II | CATG | 8 cut(s) | 55, 350, 734, 838, 962, 1109, 1228, 1342 |
| Kpn2I | TCCGGA | 1 cut(s) | 523 |
| Ksp22I | TGATCA | 2 cut(s) | 315, 640 |
| Kzo9I | GATC | 7 cut(s) | 315, 526, 619, 640, 781, 1192, 1368 |
| LmnI | GCTCC | 4 cut(s) | 392, 443, 795, 1488 |
| Lsp1109I | GCAGC | 3 cut(s) | 362, 403, 672 |
| LweI | GCATC | 1 cut(s) | 640 |
| MaeI | CTAG | 7 cut(s) | 155, 447, 456, 498, 675, 1299, 1452 |
| MaeII | ACGT | 2 cut(s) | 849, 1466 |
| MaeIII | GTNAC | 5 cut(s) | 531, 743, 1373, 1445, 1462 |
| MalI | GATC | 7 cut(s) | 317, 528, 621, 642, 783, 1194, 1370 |
| MboI | GATC | 7 cut(s) | 315, 526, 619, 640, 781, 1192, 1368 |
| MboII | GAAGA | 9 cut(s) | 256, 444, 536, 715, 930, 1212, 1270, 1273, 1385 |
| MhlI | GDGCHC | 1 cut(s) | 800 |
| MlyI | GAGTC | 2 cut(s) | 57, 1007 |
| Mph1103I | ATGCAT | 1 cut(s) | 385 |
| MroI | TCCGGA | 1 cut(s) | 523 |
| MseI | TTAA | 3 cut(s) | 948, 1286, 1501 |
| MslI | CAYNNNNRTG | 2 cut(s) | 56, 588 |
| MspA1I | CMGCKG | 1 cut(s) | 968 |
| MspI | CCGG | 2 cut(s) | 524, 863 |
| MspR9I | CCNGG | 2 cut(s) | 99, 738 |
| MvaI | CCWGG | 2 cut(s) | 99, 738 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 74, 949 |
| NdeI | CATATG | 2 cut(s) | 487, 1428 |
| NdeII | GATC | 7 cut(s) | 315, 526, 619, 640, 781, 1192, 1368 |
| NheI | GCTAGC | 1 cut(s) | 154 |
| NlaIII | CATG | 8 cut(s) | 55, 350, 734, 838, 962, 1109, 1228, 1342 |
| NmuCI | GTSAC | 3 cut(s) | 531, 743, 1462 |
| NsiI | ATGCAT | 1 cut(s) | 385 |
| NspI | RCATGY | 1 cut(s) | 350 |
| OliI | CACNNNNGTG | 1 cut(s) | 588 |
| PagI | TCATGA | 1 cut(s) | 834 |
| PfeI | GAWTC | 2 cut(s) | 171, 671 |
| PkrI | GCNGC | 3 cut(s) | 352, 418, 687 |
| PleI | GAGTC | 2 cut(s) | 56, 1007 |
| PmaCI | CACGTG | 1 cut(s) | 1467 |
| PmlI | CACGTG | 1 cut(s) | 1467 |
| PpsI | GAGTC | 2 cut(s) | 56, 1007 |
| Ppu21I | YACGTR | 1 cut(s) | 1467 |
| Psp6I | CCWGG | 2 cut(s) | 97, 736 |
| PspCI | CACGTG | 1 cut(s) | 1467 |
| PspGI | CCWGG | 2 cut(s) | 97, 736 |
| PspPI | GGNCC | 1 cut(s) | 821 |
| PvuII | CAGCTG | 1 cut(s) | 968 |
| RsaI | GTAC | 5 cut(s) | 39, 496, 1068, 1324, 1475 |
| RsaNI | GTAC | 5 cut(s) | 38, 495, 1067, 1323, 1474 |
| RseI | CAYNNNNRTG | 2 cut(s) | 56, 588 |
| SaqAI | TTAA | 3 cut(s) | 948, 1286, 1501 |
| SatI | GCNGC | 3 cut(s) | 351, 417, 686 |
| Sau3AI | GATC | 7 cut(s) | 315, 526, 619, 640, 781, 1192, 1368 |
| Sau96I | GGNCC | 1 cut(s) | 821 |
| SchI | GAGTC | 2 cut(s) | 57, 1007 |
| ScrFI | CCNGG | 2 cut(s) | 99, 738 |
| SduI | GDGCHC | 1 cut(s) | 800 |
| SfaNI | GCATC | 1 cut(s) | 640 |
| SfcI | CTRYAG | 3 cut(s) | 238, 274, 1160 |
| SinI | GGWCC | 1 cut(s) | 821 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 56, 588 |
| SpeI | ACTAGT | 2 cut(s) | 497, 1298 |
| SspMI | CTAG | 7 cut(s) | 155, 447, 456, 498, 675, 1299, 1452 |
| StyD4I | CCNGG | 2 cut(s) | 97, 736 |
| StyI | CCWWGG | 2 cut(s) | 27, 354 |
| TaaI | ACNGT | 5 cut(s) | 21, 211, 427, 657, 1022 |
| TaiI | ACGT | 2 cut(s) | 852, 1469 |
| TatI | WGTACW | 1 cut(s) | 1473 |
| TfiI | GAWTC | 2 cut(s) | 171, 671 |
| Tru1I | TTAA | 3 cut(s) | 948, 1286, 1501 |
| Tru9I | TTAA | 3 cut(s) | 948, 1286, 1501 |
| TscAI | CASTG | 2 cut(s) | 216, 262 |
| TseFI | GTSAC | 3 cut(s) | 531, 743, 1462 |
| TseI | GCWGC | 3 cut(s) | 350, 416, 685 |
| Tsp45I | GTSAC | 3 cut(s) | 531, 743, 1462 |
| TspDTI | ATGAA | 8 cut(s) | 823, 851, 1045, 1107, 1213, 1280, 1327, 1369 |
| TspRI | CASTG | 2 cut(s) | 216, 262 |
| VpaK11BI | GGWCC | 1 cut(s) | 821 |
| XapI | RAATTY | 5 cut(s) | 7, 128, 762, 1095, 1219 |
| XceI | RCATGY | 1 cut(s) | 350 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1493 |
| XmiI | GTMKAC | 1 cut(s) | 1420 |
| XspI | CTAG | 7 cut(s) | 155, 447, 456, 498, 675, 1299, 1452 |
| Zsp2I | ATGCAT | 1 cut(s) | 385 |