pycom10g04820
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0001357
Physical Location & Seq
Reverse (-)
5101774 .. 5102364
591 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g04820.2

Sequence Viewer

Length: 549 bp
ATGTTCTCTCGGGGAAAGTCTTCTGCAACAGGTGCTAACAGGCAGTTTTATCAAGGAGACAGTTCATATGGTTCTAGTTCTAATGATCAAACCTATAAGGGTGTAGGATTTGTTGGGCAATATCAAAGGAACTCTGGTAATGGTGGAAATATATCTAATAGGGGAAGTTTTTCACAATATCAACAGTTTCAAGGACCAAGAAATAACAATGGCCCTAGATTCAATAAAAGATCAAGGTTGAATGAAGGAGGCAACTTTGGAGGATTTAATAACATAAGTGGTGGTTTTTGTGGCTCTTCTACTAATAACAAGGGCAATTCAAATTGGAATTCTTGGAATGGAAGTAGTGGACAGAGATCTAATATAATACCAGAGTGTTGGATTTGCAGCAAGAGGGGCCACACTGCTTCTAACTGCTACTACAGGAATGGAAAAACTGTTTCTAATGCTCTAGTTCCCAGGTGCCAATTTTGTGGTAAACGTGGACACATTACCCTCAACTGCTTCCATAGAGGAAATTACTCATACCAAGGAGCTCAACCATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 Retrotran_gag_3 Retrotrans_gag gag_pre-integrs rve

Protein Analysis

183

Amino Acids

19.97

Weight (kDa)

10.14

Isoelectric Point (pI)

40.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 462
AcsI RAATTY 1 cut(s) 328
AgsI TTSAA 4 cut(s) 191, 223, 241, 321
AjnI CCWGG 1 cut(s) 458
AluBI AGCT 1 cut(s) 536
AluI AGCT 1 cut(s) 536
Alw21I GWGCWC 1 cut(s) 538
Alw26I GTCTC 1 cut(s) 51
Ama87I CYCGRG 1 cut(s) 9
AoxI GGCC 2 cut(s) 211, 397
ApeKI GCWGC 1 cut(s) 387
ApoI RAATTY 1 cut(s) 328
Asp700I GAANNNNTTC 2 cut(s) 19, 169
AspS9I GGNCC 3 cut(s) 194, 212, 397
AvaI CYCGRG 1 cut(s) 9
AvaII GGWCC 1 cut(s) 194
BanI GGYRCC 1 cut(s) 462
BanII GRGCYC 1 cut(s) 538
BbsI GAAGAC 1 cut(s) 12
Bbv12I GWGCWC 1 cut(s) 538
BbvI GCAGC 1 cut(s) 399
BciT130I CCWGG 1 cut(s) 460
BclI TGATCA 1 cut(s) 85
BcoDI GTCTC 1 cut(s) 51
BfaI CTAG 3 cut(s) 75, 216, 452
BfmI CTRYAG 1 cut(s) 421
BglII AGATCT 1 cut(s) 356
BisI GCNGC 1 cut(s) 388
BlsI GCNGC 1 cut(s) 389
Bme1390I CCNGG 1 cut(s) 460
Bme18I GGWCC 1 cut(s) 194
BmeT110I CYCGRG 1 cut(s) 9
BmgT120I GGNCC 3 cut(s) 194, 212, 397
BmiI GGNNCC 2 cut(s) 398, 464
BmrFI CCNGG 1 cut(s) 460
BpiI GAAGAC 1 cut(s) 12
BsaJI CCNNGG 2 cut(s) 458, 529
BseBI CCWGG 1 cut(s) 460
BseDI CCNNGG 2 cut(s) 458, 529
BseXI GCAGC 1 cut(s) 399
BshFI GGCC 2 cut(s) 213, 399
BshNI GGYRCC 1 cut(s) 462
BsiHKAI GWGCWC 1 cut(s) 538
BsiHKCI CYCGRG 1 cut(s) 9
BsmAI GTCTC 1 cut(s) 51
BsnI GGCC 2 cut(s) 213, 399
BsoBI CYCGRG 1 cut(s) 9
Bsp1286I GDGCHC 1 cut(s) 538
Bsp143I GATC 3 cut(s) 85, 230, 356
BspANI GGCC 2 cut(s) 213, 399
BspLI GGNNCC 2 cut(s) 398, 464
BspQI GCTCTTC 1 cut(s) 301
BspT107I GGYRCC 1 cut(s) 462
BssECI CCNNGG 2 cut(s) 458, 529
BssMI GATC 3 cut(s) 85, 230, 356
BssT1I CCWWGG 1 cut(s) 529
Bst2UI CCWGG 1 cut(s) 460
Bst4CI ACNGT 3 cut(s) 62, 186, 439
Bst6I CTCTTC 1 cut(s) 301
BstAPI GCANNNNNTGC 1 cut(s) 32
BstKTI GATC 3 cut(s) 88, 233, 359
BstMAI GTCTC 1 cut(s) 51
BstMBI GATC 3 cut(s) 85, 230, 356
BstMWI GCNNNNNNNGC 2 cut(s) 32, 396
BstNI CCWGG 1 cut(s) 460
BstSCI CCNGG 1 cut(s) 458
BstSFI CTRYAG 1 cut(s) 421
BstV1I GCAGC 1 cut(s) 399
BstV2I GAAGAC 1 cut(s) 12
BstX2I RGATCY 1 cut(s) 356
BstXI CCANNNNNNTGG 2 cut(s) 378, 473
BstYI RGATCY 1 cut(s) 356
BsuRI GGCC 2 cut(s) 213, 399
BtsI GCAGTG 1 cut(s) 402
BtsIMutI CAGTG 1 cut(s) 402
Cfr13I GGNCC 3 cut(s) 194, 212, 397
CviJI RGCY 4 cut(s) 213, 294, 399, 536
CviKI_1 RGCY 4 cut(s) 213, 294, 399, 536
DpnI GATC 3 cut(s) 87, 232, 358
DpnII GATC 3 cut(s) 85, 230, 356
Eam1104I CTCTTC 1 cut(s) 301
EarI CTCTTC 1 cut(s) 301
Ecl136II GAGCTC 1 cut(s) 536
Eco130I CCWWGG 1 cut(s) 529
Eco24I GRGCYC 1 cut(s) 538
Eco47I GGWCC 1 cut(s) 194
Eco53kI GAGCTC 1 cut(s) 536
Eco88I CYCGRG 1 cut(s) 9
EcoICRI GAGCTC 1 cut(s) 536
EcoRI GAATTC 1 cut(s) 328
EcoRII CCWGG 1 cut(s) 458
EcoT14I CCWWGG 1 cut(s) 529
EcoT38I GRGCYC 1 cut(s) 538
ErhI CCWWGG 1 cut(s) 529
FaiI YATR 8 cut(s) 67, 69, 96, 152, 275, 365, 510, 526
FauNDI CATATG 1 cut(s) 67
FbaI TGATCA 1 cut(s) 85
Fnu4HI GCNGC 1 cut(s) 388
FriOI GRGCYC 1 cut(s) 538
Fsp4HI GCNGC 1 cut(s) 388
FspBI CTAG 3 cut(s) 75, 216, 452
GluI GCNGC 1 cut(s) 388
HaeIII GGCC 2 cut(s) 213, 399
HinfI GANTC 1 cut(s) 219
Hpy166II GTNNAC 3 cut(s) 350, 479, 485
Hpy8I GTNNAC 3 cut(s) 350, 479, 485
HpyAV CCTTC 1 cut(s) 239
HpyCH4III ACNGT 3 cut(s) 62, 186, 439
HpyCH4IV ACGT 1 cut(s) 481
HpyCH4V TGCA 2 cut(s) 26, 387
HpyF10VI GCNNNNNNNGC 2 cut(s) 32, 396
HpySE526I ACGT 1 cut(s) 481
Ksp22I TGATCA 1 cut(s) 85
Kzo9I GATC 3 cut(s) 85, 230, 356
LguI GCTCTTC 1 cut(s) 301
LmnI GCTCC 1 cut(s) 533
LpnPI CCDG 7 cut(s) 15, 25, 120, 384, 409, 445, 472
Lsp1109I GCAGC 1 cut(s) 399
MaeI CTAG 3 cut(s) 75, 216, 452
MaeII ACGT 1 cut(s) 481
MalI GATC 3 cut(s) 87, 232, 358
MboI GATC 3 cut(s) 85, 230, 356
MboII GAAGA 2 cut(s) 12, 288
MflI RGATCY 1 cut(s) 356
MhlI GDGCHC 1 cut(s) 538
MluCI AATT 5 cut(s) 316, 322, 328, 467, 517
MmeI TCCRAC 1 cut(s) 359
MnlI CCTC 5 cut(s) 242, 254, 387, 506, 506
MroXI GAANNNNTTC 2 cut(s) 19, 169
MseI TTAA 2 cut(s) 267, 547
MspR9I CCNGG 1 cut(s) 460
MvaI CCWGG 1 cut(s) 460
MwoI GCNNNNNNNGC 2 cut(s) 32, 396
NdeI CATATG 1 cut(s) 67
NdeII GATC 3 cut(s) 85, 230, 356
NlaIV GGNNCC 2 cut(s) 398, 464
PciSI GCTCTTC 1 cut(s) 301
PdmI GAANNNNTTC 2 cut(s) 19, 169
PfeI GAWTC 1 cut(s) 219
PkrI GCNGC 1 cut(s) 389
Psp124BI GAGCTC 1 cut(s) 538
Psp6I CCWGG 1 cut(s) 458
PspGI CCWGG 1 cut(s) 458
PspN4I GGNNCC 2 cut(s) 398, 464
PspPI GGNCC 3 cut(s) 194, 212, 397
PsuI RGATCY 1 cut(s) 356
SacI GAGCTC 1 cut(s) 538
SapI GCTCTTC 1 cut(s) 301
SaqAI TTAA 2 cut(s) 267, 547
SatI GCNGC 1 cut(s) 388
Sau3AI GATC 3 cut(s) 85, 230, 356
Sau96I GGNCC 3 cut(s) 194, 212, 397
ScrFI CCNGG 1 cut(s) 460
SduI GDGCHC 1 cut(s) 538
SetI ASST 6 cut(s) 34, 95, 239, 464, 484, 538
SfcI CTRYAG 1 cut(s) 421
SinI GGWCC 1 cut(s) 194
Sse9I AATT 5 cut(s) 316, 322, 328, 467, 517
SspMI CTAG 3 cut(s) 75, 216, 452
SstI GAGCTC 1 cut(s) 538
StyD4I CCNGG 1 cut(s) 458
StyI CCWWGG 1 cut(s) 529
TaaI ACNGT 3 cut(s) 62, 186, 439
TaiI ACGT 1 cut(s) 484
TasI AATT 5 cut(s) 316, 322, 328, 467, 517
TfiI GAWTC 1 cut(s) 219
Tru1I TTAA 2 cut(s) 267, 547
Tru9I TTAA 2 cut(s) 267, 547
TscAI CASTG 1 cut(s) 409
TseI GCWGC 1 cut(s) 387
TspDTI ATGAA 2 cut(s) 54, 258
TspRI CASTG 1 cut(s) 409
VpaK11BI GGWCC 1 cut(s) 194
XapI RAATTY 1 cut(s) 328
XmnI GAANNNNTTC 2 cut(s) 19, 169
XspI CTAG 3 cut(s) 75, 216, 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.