Catalytic subunit of pyrophosphate--fructose 6-phosphate 1-phosphotransferase. Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis. Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| PFK | PF00365 | 99 - 464 | 2.7e-36 | Phosphofructokinase |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 984 |
| AccB1I | GGYRCC | 2 cut(s) | 58, 253 |
| AccI | GTMKAC | 3 cut(s) | 87, 165, 277 |
| AccII | CGCG | 2 cut(s) | 209, 378 |
| AciI | CCGC | 8 cut(s) | 30, 174, 183, 186, 376, 411, 836, 1389 |
| AclWI | GGATC | 2 cut(s) | 431, 1181 |
| AcoI | YGGCCR | 3 cut(s) | 70, 1291, 1651 |
| AcsI | RAATTY | 4 cut(s) | 354, 1065, 1441, 1580 |
| AcuI | CTGAAG | 1 cut(s) | 1173 |
| AcyI | GRCGYC | 1 cut(s) | 254 |
| AdeI | CACNNNGTG | 2 cut(s) | 343, 949 |
| AfaI | GTAC | 1 cut(s) | 819 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 68 |
| AjiI | CACGTC | 1 cut(s) | 1504 |
| AjnI | CCWGG | 3 cut(s) | 99, 1203, 1630 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 557, 589, 1455, 1487 |
| AluBI | AGCT | 5 cut(s) | 180, 568, 919, 1135, 1397 |
| AluI | AGCT | 5 cut(s) | 180, 568, 919, 1135, 1397 |
| Alw21I | GWGCWC | 3 cut(s) | 144, 1399, 1570 |
| Alw26I | GTCTC | 2 cut(s) | 1244, 1507 |
| Alw44I | GTGCAC | 1 cut(s) | 1566 |
| AlwI | GGATC | 2 cut(s) | 431, 1181 |
| Ama87I | CYCGRG | 3 cut(s) | 329, 455, 1031 |
| AoxI | GGCC | 7 cut(s) | 70, 102, 319, 413, 1071, 1291, 1651 |
| ApaLI | GTGCAC | 1 cut(s) | 1566 |
| ApeKI | GCWGC | 3 cut(s) | 35, 908, 1039 |
| ApoI | RAATTY | 4 cut(s) | 354, 1065, 1441, 1580 |
| AseI | ATTAAT | 1 cut(s) | 1496 |
| AspLEI | GCGC | 3 cut(s) | 256, 328, 380 |
| AspS9I | GGNCC | 6 cut(s) | 97, 170, 413, 715, 1072, 1634 |
| AsuC2I | CCSGG | 3 cut(s) | 330, 331, 790 |
| AsuHPI | GGTGA | 3 cut(s) | 58, 658, 707 |
| AsuNHI | GCTAGC | 1 cut(s) | 176 |
| AvaI | CYCGRG | 3 cut(s) | 329, 455, 1031 |
| AvaII | GGWCC | 4 cut(s) | 97, 170, 715, 1634 |
| AxyI | CCTNAGG | 2 cut(s) | 1008, 1592 |
| BaeGI | GKGCMC | 2 cut(s) | 339, 1570 |
| BalI | TGGCCA | 2 cut(s) | 1293, 1653 |
| BanI | GGYRCC | 2 cut(s) | 58, 253 |
| BanII | GRGCYC | 1 cut(s) | 1399 |
| BauI | CACGAG | 1 cut(s) | 663 |
| Bbv12I | GWGCWC | 3 cut(s) | 144, 1399, 1570 |
| BbvI | GCAGC | 3 cut(s) | 22, 895, 1051 |
| BccI | CCATC | 4 cut(s) | 19, 247, 572, 1088 |
| BceAI | ACGGC | 3 cut(s) | 43, 545, 1497 |
| BciT130I | CCWGG | 3 cut(s) | 101, 1205, 1632 |
| BcnI | CCSGG | 3 cut(s) | 330, 331, 790 |
| BcoDI | GTCTC | 2 cut(s) | 1244, 1507 |
| BfaI | CTAG | 2 cut(s) | 177, 1130 |
| BfmI | CTRYAG | 1 cut(s) | 88 |
| BfoI | RGCGCY | 2 cut(s) | 257, 329 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 334 |
| BisI | GCNGC | 6 cut(s) | 36, 186, 837, 909, 1040, 1389 |
| BlsI | GCNGC | 6 cut(s) | 37, 187, 838, 910, 1041, 1390 |
| Bme1390I | CCNGG | 6 cut(s) | 101, 330, 331, 790, 1205, 1632 |
| Bme18I | GGWCC | 4 cut(s) | 97, 170, 715, 1634 |
| BmeT110I | CYCGRG | 3 cut(s) | 329, 455, 1031 |
| BmgBI | CACGTC | 1 cut(s) | 1504 |
| BmgT120I | GGNCC | 6 cut(s) | 97, 170, 413, 715, 1072, 1634 |
| BmiI | GGNNCC | 5 cut(s) | 60, 172, 255, 717, 1636 |
| BmrFI | CCNGG | 6 cut(s) | 101, 330, 331, 790, 1205, 1632 |
| BmrI | ACTGGG | 2 cut(s) | 1428, 1612 |
| BmsI | GCATC | 6 cut(s) | 775, 1164, 1221, 1438, 1595, 1667 |
| BmtI | GCTAGC | 1 cut(s) | 180 |
| BmuI | ACTGGG | 2 cut(s) | 1428, 1612 |
| BpmI | CTGGAG | 3 cut(s) | 336, 513, 1413 |
| BpuMI | CCSGG | 3 cut(s) | 330, 331, 790 |
| Bsa29I | ATCGAT | 1 cut(s) | 114 |
| BsaHI | GRCGYC | 1 cut(s) | 254 |
| BsaJI | CCNNGG | 5 cut(s) | 329, 330, 976, 1032, 1300 |
| BsaWI | WCCGGW | 1 cut(s) | 280 |
| BsaXI | ACNNNNNCTCC | 9 cut(s) | 114, 144, 275, 305, 888, 918, 1504, 1534, 1689 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 68 |
| Bse118I | RCCGGY | 3 cut(s) | 38, 68, 188 |
| Bse1I | ACTGG | 4 cut(s) | 105, 802, 1423, 1607 |
| Bse21I | CCTNAGG | 2 cut(s) | 1008, 1592 |
| Bse3DI | GCAATG | 2 cut(s) | 778, 1554 |
| BseBI | CCWGG | 3 cut(s) | 101, 1205, 1632 |
| BseCI | ATCGAT | 1 cut(s) | 114 |
| BseDI | CCNNGG | 5 cut(s) | 329, 330, 976, 1032, 1300 |
| BseGI | GGATG | 5 cut(s) | 583, 874, 1453, 1586, 1658 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 68 |
| BseMI | GCAATG | 2 cut(s) | 778, 1554 |
| BseMII | CTCAG | 4 cut(s) | 157, 999, 1583, 1684 |
| BseNI | ACTGG | 4 cut(s) | 105, 802, 1423, 1607 |
| BseRI | GAGGAG | 1 cut(s) | 215 |
| BseSI | GKGCMC | 2 cut(s) | 339, 1570 |
| BseX3I | CGGCCG | 1 cut(s) | 70 |
| BseXI | GCAGC | 3 cut(s) | 22, 895, 1051 |
| BseYI | CCCAGC | 1 cut(s) | 419 |
| BsgI | GTGCAG | 2 cut(s) | 228, 1058 |
| Bsh1236I | CGCG | 2 cut(s) | 209, 378 |
| Bsh1285I | CGRYCG | 1 cut(s) | 73 |
| BshFI | GGCC | 7 cut(s) | 72, 104, 321, 415, 1073, 1293, 1653 |
| BshNI | GGYRCC | 2 cut(s) | 58, 253 |
| BshVI | ATCGAT | 1 cut(s) | 114 |
| BsiEI | CGRYCG | 1 cut(s) | 73 |
| BsiHKAI | GWGCWC | 3 cut(s) | 144, 1399, 1570 |
| BsiHKCI | CYCGRG | 3 cut(s) | 329, 455, 1031 |
| BsiSI | CCGG | 7 cut(s) | 39, 56, 69, 189, 281, 330, 790 |
| BslFI | GGGAC | 4 cut(s) | 156, 527, 701, 1620 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 68 |
| BsmAI | GTCTC | 2 cut(s) | 1244, 1507 |
| BsmFI | GGGAC | 4 cut(s) | 156, 527, 701, 1620 |
| BsnI | GGCC | 7 cut(s) | 72, 104, 321, 415, 1073, 1293, 1653 |
| BsoBI | CYCGRG | 3 cut(s) | 329, 455, 1031 |
| Bsp1286I | GDGCHC | 4 cut(s) | 144, 339, 1399, 1570 |
| Bsp143I | GATC | 5 cut(s) | 115, 204, 297, 423, 1186 |
| Bsp68I | TCGCGA | 1 cut(s) | 209 |
| BspACI | CCGC | 8 cut(s) | 30, 174, 183, 186, 376, 411, 836, 1389 |
| BspANI | GGCC | 7 cut(s) | 72, 104, 321, 415, 1073, 1293, 1653 |
| BspCNI | CTCAG | 4 cut(s) | 156, 1000, 1584, 1683 |
| BspDI | ATCGAT | 1 cut(s) | 114 |
| BspFNI | CGCG | 2 cut(s) | 209, 378 |
| BspHI | TCATGA | 2 cut(s) | 426, 777 |
| BspLI | GGNNCC | 5 cut(s) | 60, 172, 255, 717, 1636 |
| BspOI | GCTAGC | 1 cut(s) | 180 |
| BspPI | GGATC | 2 cut(s) | 431, 1181 |
| BspT107I | GGYRCC | 2 cut(s) | 58, 253 |
| BsrDI | GCAATG | 2 cut(s) | 778, 1554 |
| BsrFI | RCCGGY | 3 cut(s) | 38, 68, 188 |
| BsrI | ACTGG | 4 cut(s) | 105, 802, 1423, 1607 |
| BssAI | RCCGGY | 3 cut(s) | 38, 68, 188 |
| BssECI | CCNNGG | 5 cut(s) | 329, 330, 976, 1032, 1300 |
| BssMI | GATC | 5 cut(s) | 115, 204, 297, 423, 1186 |
| BssNI | GRCGYC | 1 cut(s) | 254 |
| BssSI | CACGAG | 1 cut(s) | 663 |
| BssT1I | CCWWGG | 2 cut(s) | 976, 1300 |
| Bst2BI | CACGAG | 1 cut(s) | 663 |
| Bst2UI | CCWGG | 3 cut(s) | 101, 1205, 1632 |
| Bst4CI | ACNGT | 7 cut(s) | 170, 249, 646, 695, 1409, 1471, 1619 |
| Bst6I | CTCTTC | 2 cut(s) | 894, 1259 |
| BstACI | GRCGYC | 1 cut(s) | 254 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1174 |
| BstC8I | GCNNGC | 8 cut(s) | 178, 190, 413, 417, 622, 745, 834, 969 |
| BstDEI | CTNAG | 4 cut(s) | 143, 1008, 1592, 1670 |
| BstF5I | GGATG | 5 cut(s) | 583, 874, 1453, 1586, 1658 |
| BstFNI | CGCG | 2 cut(s) | 209, 378 |
| BstH2I | RGCGCY | 2 cut(s) | 257, 329 |
| BstHHI | GCGC | 3 cut(s) | 256, 328, 380 |
| BstKTI | GATC | 5 cut(s) | 118, 207, 300, 426, 1189 |
| BstMAI | GTCTC | 2 cut(s) | 1244, 1507 |
| BstMBI | GATC | 5 cut(s) | 115, 204, 297, 423, 1186 |
| BstMCI | CGRYCG | 1 cut(s) | 73 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 148, 182, 251, 334, 1174, 1394, 1664 |
| BstNI | CCWGG | 3 cut(s) | 101, 1205, 1632 |
| BstSCI | CCNGG | 6 cut(s) | 99, 328, 329, 788, 1203, 1630 |
| BstSFI | CTRYAG | 1 cut(s) | 88 |
| BstSLI | GKGCMC | 2 cut(s) | 339, 1570 |
| BstUI | CGCG | 2 cut(s) | 209, 378 |
| BstV1I | GCAGC | 3 cut(s) | 22, 895, 1051 |
| BstX2I | RGATCY | 1 cut(s) | 1186 |
| BstYI | RGATCY | 1 cut(s) | 1186 |
| BstZI | CGGCCG | 1 cut(s) | 70 |
| Bsu15I | ATCGAT | 1 cut(s) | 114 |
| Bsu36I | CCTNAGG | 2 cut(s) | 1008, 1592 |
| BsuRI | GGCC | 7 cut(s) | 72, 104, 321, 415, 1073, 1293, 1653 |
| BsuTUI | ATCGAT | 1 cut(s) | 114 |
| BtrI | CACGTC | 1 cut(s) | 1504 |
| BtsCI | GGATG | 5 cut(s) | 583, 874, 1453, 1586, 1658 |
| BtsI | GCAGTG | 2 cut(s) | 511, 738 |
| BtsIMutI | CAGTG | 7 cut(s) | 14, 245, 511, 738, 923, 936, 1414 |
| BtuMI | TCGCGA | 1 cut(s) | 209 |
| Cac8I | GCNNGC | 8 cut(s) | 178, 190, 413, 417, 622, 745, 834, 969 |
| CciI | TCATGA | 2 cut(s) | 426, 777 |
| CfoI | GCGC | 3 cut(s) | 256, 328, 380 |
| Cfr10I | RCCGGY | 3 cut(s) | 38, 68, 188 |
| Cfr13I | GGNCC | 6 cut(s) | 97, 170, 413, 715, 1072, 1634 |
| Cfr9I | CCCGGG | 1 cut(s) | 329 |
| ClaI | ATCGAT | 1 cut(s) | 114 |
| Csp6I | GTAC | 1 cut(s) | 818 |
| CviAII | CATG | 7 cut(s) | 119, 427, 778, 1076, 1193, 1517, 1649 |
| CviQI | GTAC | 1 cut(s) | 818 |
| DdeI | CTNAG | 4 cut(s) | 143, 1008, 1592, 1670 |
| DinI | GGCGCC | 1 cut(s) | 255 |
| DpnI | GATC | 5 cut(s) | 117, 206, 299, 425, 1188 |
| DpnII | GATC | 5 cut(s) | 115, 204, 297, 423, 1186 |
| DraI | TTTAAA | 1 cut(s) | 541 |
| DraIII | CACNNNGTG | 2 cut(s) | 343, 949 |
| EaeI | YGGCCR | 3 cut(s) | 70, 1291, 1651 |
| EagI | CGGCCG | 1 cut(s) | 70 |
| Eam1104I | CTCTTC | 2 cut(s) | 894, 1259 |
| EarI | CTCTTC | 2 cut(s) | 894, 1259 |
| EciI | GGCGGA | 1 cut(s) | 172 |
| Ecl136II | GAGCTC | 1 cut(s) | 1397 |
| EclXI | CGGCCG | 1 cut(s) | 70 |
| Eco130I | CCWWGG | 2 cut(s) | 976, 1300 |
| Eco24I | GRGCYC | 1 cut(s) | 1399 |
| Eco32I | GATATC | 1 cut(s) | 1428 |
| Eco47I | GGWCC | 4 cut(s) | 97, 170, 715, 1634 |
| Eco52I | CGGCCG | 1 cut(s) | 70 |
| Eco53kI | GAGCTC | 1 cut(s) | 1397 |
| Eco57I | CTGAAG | 1 cut(s) | 1173 |
| Eco81I | CCTNAGG | 2 cut(s) | 1008, 1592 |
| Eco88I | CYCGRG | 3 cut(s) | 329, 455, 1031 |
| EcoICRI | GAGCTC | 1 cut(s) | 1397 |
| EcoO109I | RGGNCCY | 1 cut(s) | 715 |
| EcoRII | CCWGG | 3 cut(s) | 99, 1203, 1630 |
| EcoRV | GATATC | 1 cut(s) | 1428 |
| EcoT14I | CCWWGG | 2 cut(s) | 976, 1300 |
| EcoT22I | ATGCAT | 1 cut(s) | 1378 |
| EcoT38I | GRGCYC | 1 cut(s) | 1399 |
| EgeI | GGCGCC | 1 cut(s) | 255 |
| EheI | GGCGCC | 1 cut(s) | 255 |
| ErhI | CCWWGG | 2 cut(s) | 976, 1300 |
| FaeI | CATG | 7 cut(s) | 122, 430, 781, 1079, 1196, 1520, 1652 |
| FaqI | GGGAC | 4 cut(s) | 156, 527, 701, 1620 |
| FatI | CATG | 7 cut(s) | 118, 426, 777, 1075, 1192, 1516, 1648 |
| FauI | CCCGC | 2 cut(s) | 181, 404 |
| FblI | GTMKAC | 3 cut(s) | 87, 165, 277 |
| Fnu4HI | GCNGC | 6 cut(s) | 36, 186, 837, 909, 1040, 1389 |
| FokI | GGATG | 5 cut(s) | 590, 861, 1460, 1573, 1645 |
| FriOI | GRGCYC | 1 cut(s) | 1399 |
| Fsp4HI | GCNGC | 6 cut(s) | 36, 186, 837, 909, 1040, 1389 |
| FspBI | CTAG | 2 cut(s) | 177, 1130 |
| GlaI | GCGC | 3 cut(s) | 255, 327, 379 |
| GluI | GCNGC | 6 cut(s) | 36, 186, 837, 909, 1040, 1389 |
| GsaI | CCCAGC | 1 cut(s) | 423 |
| GsuI | CTGGAG | 3 cut(s) | 336, 513, 1413 |
| HaeII | RGCGCY | 2 cut(s) | 257, 329 |
| HaeIII | GGCC | 7 cut(s) | 72, 104, 321, 415, 1073, 1293, 1653 |
| HapII | CCGG | 7 cut(s) | 39, 56, 69, 189, 281, 330, 790 |
| HhaI | GCGC | 3 cut(s) | 256, 328, 380 |
| Hin1I | GRCGYC | 1 cut(s) | 254 |
| Hin1II | CATG | 7 cut(s) | 122, 430, 781, 1079, 1196, 1520, 1652 |
| Hin6I | GCGC | 3 cut(s) | 254, 326, 378 |
| HinP1I | GCGC | 3 cut(s) | 254, 326, 378 |
| HincII | GTYRAC | 2 cut(s) | 166, 795 |
| HindII | GTYRAC | 2 cut(s) | 166, 795 |
| HindIII | AAGCTT | 1 cut(s) | 566 |
| HinfI | GANTC | 8 cut(s) | 47, 75, 263, 269, 499, 524, 605, 1358 |
| HpaII | CCGG | 7 cut(s) | 39, 56, 69, 189, 281, 330, 790 |
| HphI | GGTGA | 3 cut(s) | 58, 658, 707 |
| Hpy166II | GTNNAC | 6 cut(s) | 88, 166, 278, 795, 1343, 1568 |
| Hpy188I | TCNGA | 3 cut(s) | 146, 268, 1697 |
| Hpy8I | GTNNAC | 6 cut(s) | 88, 166, 278, 795, 1343, 1568 |
| Hpy99I | CGWCG | 2 cut(s) | 167, 170 |
| HpyAV | CCTTC | 8 cut(s) | 144, 163, 504, 1271, 1322, 1412, 1537, 1556 |
| HpyCH4III | ACNGT | 7 cut(s) | 170, 249, 646, 695, 1409, 1471, 1619 |
| HpyCH4IV | ACGT | 1 cut(s) | 1503 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 148, 182, 251, 334, 1174, 1394, 1664 |
| HpyF3I | CTNAG | 4 cut(s) | 143, 1008, 1592, 1670 |
| HpySE526I | ACGT | 1 cut(s) | 1503 |
| Hsp92I | GRCGYC | 1 cut(s) | 254 |
| Hsp92II | CATG | 7 cut(s) | 122, 430, 781, 1079, 1196, 1520, 1652 |
| HspAI | GCGC | 3 cut(s) | 254, 326, 378 |
| KasI | GGCGCC | 1 cut(s) | 253 |
| KroI | GCCGGC | 1 cut(s) | 188 |
| KroNI | GCCGGC | 1 cut(s) | 190 |
| Kzo9I | GATC | 5 cut(s) | 115, 204, 297, 423, 1186 |
| LmnI | GCTCC | 3 cut(s) | 185, 283, 1394 |
| Lsp1109I | GCAGC | 3 cut(s) | 22, 895, 1051 |
| LweI | GCATC | 6 cut(s) | 775, 1164, 1221, 1438, 1595, 1667 |
| MaeI | CTAG | 2 cut(s) | 177, 1130 |
| MaeII | ACGT | 1 cut(s) | 1503 |
| MaeIII | GTNAC | 4 cut(s) | 64, 695, 934, 1619 |
| MalI | GATC | 5 cut(s) | 117, 206, 299, 425, 1188 |
| MboI | GATC | 5 cut(s) | 115, 204, 297, 423, 1186 |
| MboII | GAAGA | 8 cut(s) | 307, 661, 911, 1103, 1240, 1276, 1344, 1472 |
| MflI | RGATCY | 1 cut(s) | 1186 |
| MhlI | GDGCHC | 4 cut(s) | 144, 339, 1399, 1570 |
| MlsI | TGGCCA | 2 cut(s) | 1293, 1653 |
| MluCI | AATT | 8 cut(s) | 354, 888, 1017, 1055, 1065, 1441, 1580, 1643 |
| MluNI | TGGCCA | 2 cut(s) | 1293, 1653 |
| Mly113I | GGCGCC | 1 cut(s) | 254 |
| MlyI | GAGTC | 2 cut(s) | 56, 599 |
| MmeI | TCCRAC | 1 cut(s) | 1686 |
| Mox20I | TGGCCA | 2 cut(s) | 1293, 1653 |
| Mph1103I | ATGCAT | 1 cut(s) | 1378 |
| MroNI | GCCGGC | 1 cut(s) | 188 |
| MscI | TGGCCA | 2 cut(s) | 1293, 1653 |
| MseI | TTAA | 3 cut(s) | 540, 1496, 1708 |
| MslI | CAYNNNNRTG | 2 cut(s) | 856, 1197 |
| Msp20I | TGGCCA | 2 cut(s) | 1293, 1653 |
| MspA1I | CMGCKG | 1 cut(s) | 1391 |
| MspI | CCGG | 7 cut(s) | 39, 56, 69, 189, 281, 330, 790 |
| MspR9I | CCNGG | 6 cut(s) | 101, 330, 331, 790, 1205, 1632 |
| MssI | GTTTAAAC | 1 cut(s) | 541 |
| MvaI | CCWGG | 3 cut(s) | 101, 1205, 1632 |
| MvnI | CGCG | 2 cut(s) | 209, 378 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 148, 182, 251, 334, 1174, 1394, 1664 |
| NaeI | GCCGGC | 1 cut(s) | 190 |
| NarI | GGCGCC | 1 cut(s) | 254 |
| NciI | CCSGG | 3 cut(s) | 330, 331, 790 |
| NdeII | GATC | 5 cut(s) | 115, 204, 297, 423, 1186 |
| NgoMIV | GCCGGC | 1 cut(s) | 188 |
| NheI | GCTAGC | 1 cut(s) | 176 |
| NlaIII | CATG | 7 cut(s) | 122, 430, 781, 1079, 1196, 1520, 1652 |
| NlaIV | GGNNCC | 5 cut(s) | 60, 172, 255, 717, 1636 |
| NmuCI | GTSAC | 3 cut(s) | 64, 695, 934 |
| NruI | TCGCGA | 1 cut(s) | 209 |
| NsiI | ATGCAT | 1 cut(s) | 1378 |
| PagI | TCATGA | 2 cut(s) | 426, 777 |
| PdiI | GCCGGC | 1 cut(s) | 190 |
| PfeI | GAWTC | 6 cut(s) | 75, 263, 269, 499, 524, 1358 |
| PkrI | GCNGC | 6 cut(s) | 37, 187, 838, 910, 1041, 1390 |
| PleI | GAGTC | 2 cut(s) | 55, 599 |
| PluTI | GGCGCC | 1 cut(s) | 257 |
| PmeI | GTTTAAAC | 1 cut(s) | 541 |
| PpsI | GAGTC | 2 cut(s) | 55, 599 |
| PpuMI | RGGWCCY | 1 cut(s) | 715 |
| PshBI | ATTAAT | 1 cut(s) | 1496 |
| PsiI | TTATAA | 1 cut(s) | 984 |
| Psp124BI | GAGCTC | 1 cut(s) | 1399 |
| Psp5II | RGGWCCY | 1 cut(s) | 715 |
| Psp6I | CCWGG | 3 cut(s) | 99, 1203, 1630 |
| PspFI | CCCAGC | 1 cut(s) | 419 |
| PspGI | CCWGG | 3 cut(s) | 99, 1203, 1630 |
| PspN4I | GGNNCC | 5 cut(s) | 60, 172, 255, 717, 1636 |
| PspPI | GGNCC | 6 cut(s) | 97, 170, 413, 715, 1072, 1634 |
| PspPPI | RGGWCCY | 1 cut(s) | 715 |
| PsuI | RGATCY | 1 cut(s) | 1186 |
| RruI | TCGCGA | 1 cut(s) | 209 |
| RsaI | GTAC | 1 cut(s) | 819 |
| RsaNI | GTAC | 1 cut(s) | 818 |
| RseI | CAYNNNNRTG | 2 cut(s) | 856, 1197 |
| SacI | GAGCTC | 1 cut(s) | 1399 |
| SalI | GTCGAC | 1 cut(s) | 164 |
| SaqAI | TTAA | 3 cut(s) | 540, 1496, 1708 |
| SatI | GCNGC | 6 cut(s) | 36, 186, 837, 909, 1040, 1389 |
| Sau3AI | GATC | 5 cut(s) | 115, 204, 297, 423, 1186 |
| Sau96I | GGNCC | 6 cut(s) | 97, 170, 413, 715, 1072, 1634 |
| SchI | GAGTC | 2 cut(s) | 56, 599 |
| ScrFI | CCNGG | 6 cut(s) | 101, 330, 331, 790, 1205, 1632 |
| SduI | GDGCHC | 4 cut(s) | 144, 339, 1399, 1570 |
| SfaNI | GCATC | 6 cut(s) | 775, 1164, 1221, 1438, 1595, 1667 |
| SfcI | CTRYAG | 1 cut(s) | 88 |
| SfoI | GGCGCC | 1 cut(s) | 255 |
| SgrDI | CGTCGACG | 1 cut(s) | 164 |
| SinI | GGWCC | 4 cut(s) | 97, 170, 715, 1634 |
| SmaI | CCCGGG | 1 cut(s) | 331 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 856, 1197 |
| Sse9I | AATT | 8 cut(s) | 354, 888, 1017, 1055, 1065, 1441, 1580, 1643 |
| SsiI | CCGC | 8 cut(s) | 30, 174, 183, 186, 376, 411, 836, 1389 |
| SspDI | GGCGCC | 1 cut(s) | 253 |
| SspI | AATATT | 1 cut(s) | 806 |
| SspMI | CTAG | 2 cut(s) | 177, 1130 |
| SstI | GAGCTC | 1 cut(s) | 1399 |
| StyD4I | CCNGG | 6 cut(s) | 99, 328, 329, 788, 1203, 1630 |
| StyI | CCWWGG | 2 cut(s) | 976, 1300 |
| TaaI | ACNGT | 7 cut(s) | 170, 249, 646, 695, 1409, 1471, 1619 |
| TaiI | ACGT | 1 cut(s) | 1506 |
| TaqI | TCGA | 5 cut(s) | 114, 165, 360, 444, 492 |
| TaqII | GACCGA | 1 cut(s) | 221 |
| TasI | AATT | 8 cut(s) | 354, 888, 1017, 1055, 1065, 1441, 1580, 1643 |
| TauI | GCSGC | 3 cut(s) | 188, 839, 1391 |
| TfiI | GAWTC | 6 cut(s) | 75, 263, 269, 499, 524, 1358 |
| Tru1I | TTAA | 3 cut(s) | 540, 1496, 1708 |
| Tru9I | TTAA | 3 cut(s) | 540, 1496, 1708 |
| TscAI | CASTG | 7 cut(s) | 21, 252, 511, 745, 930, 943, 1414 |
| TseFI | GTSAC | 3 cut(s) | 64, 695, 934 |
| TseI | GCWGC | 3 cut(s) | 35, 908, 1039 |
| Tsp45I | GTSAC | 3 cut(s) | 64, 695, 934 |
| TspDTI | ATGAA | 8 cut(s) | 443, 1015, 1077, 1104, 1272, 1341, 1482, 1637 |
| TspGWI | ACGGA | 2 cut(s) | 73, 127 |
| TspMI | CCCGGG | 1 cut(s) | 329 |
| TspRI | CASTG | 7 cut(s) | 21, 252, 511, 745, 930, 943, 1414 |
| VneI | GTGCAC | 1 cut(s) | 1566 |
| VpaK11BI | GGWCC | 4 cut(s) | 97, 170, 715, 1634 |
| VspI | ATTAAT | 1 cut(s) | 1496 |
| XapI | RAATTY | 4 cut(s) | 354, 1065, 1441, 1580 |
| XbaI | TCTAGA | 1 cut(s) | 1129 |
| XmaI | CCCGGG | 1 cut(s) | 329 |
| XmiI | GTMKAC | 3 cut(s) | 87, 165, 277 |
| XspI | CTAG | 2 cut(s) | 177, 1130 |
| Zsp2I | ATGCAT | 1 cut(s) | 1378 |