Rh1AG166900

Catalytic subunit of pyrophosphate--fructose 6-phosphate 1-phosphotransferase. Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis. Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0001357
Physical Location & Seq
Forward (+)
31786577 .. 31788615
2039 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG166900.1

Sequence Viewer

Length: 450 bp
ATGTATGGTTTCAGAGGTGGACCTGCTGGTATCATGAAATGCAAATACGTTGAGCTTACACCGGAGTATGTTTATCCATACAGAAATCAGGGTGGTTTTGATATGATTTATAGTGGAAGAGACAAGATTGAAACTCCAGAGCAGTTTAAGCAAGCACATGAGACAACTGTGAAGCTTGATTTGGATGATCTTGTTGTGATTGAAGGAGATGACTCAAACGGTGTTATACTTATTCCTGAAGGTCTTATTGACTTCATTCCTGAGGAAGCAGATACATCGACAATAAAGCTTTGCTTGAATTGGAGCAAGGCAGTGCTCTCCTCATCATGGAGAGATGGCTACAATGAGGATAAAGACTTGCAAGCACAAGATGAAACAAGAAGACAAATAATAAGCACAGTGTGGATCAAGCCATGGTTTGGTTTTGCACTTAAGAGTCTCAGACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko01100 ko01110 ko01120 ko01130 map00010 map00030 map00051 map01100 map01110 map01120 map01130
Pfam Domains
Protein Families
PFK

Protein Analysis

149

Amino Acids

17.06

Weight (kDa)

4.73

Isoelectric Point (pI)

35.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 31
AccB7I CCANNNNNTGG 1 cut(s) 419
AclWI GGATC 1 cut(s) 413
AcuI CTGAAG 1 cut(s) 258
AdeI CACNNNGTG 1 cut(s) 402
AfiI CCNNNNNNNGG 2 cut(s) 327, 419
AflII CTTAAG 1 cut(s) 431
AgsI TTSAA 3 cut(s) 131, 203, 298
AjuI GAANNNNNNNTTGG 2 cut(s) 164, 196
AluBI AGCT 3 cut(s) 55, 175, 289
AluI AGCT 3 cut(s) 55, 175, 289
Alw21I GWGCWC 1 cut(s) 318
Alw26I GTCTC 3 cut(s) 114, 155, 443
AlwI GGATC 1 cut(s) 413
AspS9I GGNCC 1 cut(s) 20
AvaII GGWCC 1 cut(s) 20
AxyI CCTNAGG 1 cut(s) 261
BbsI GAAGAC 1 cut(s) 388
Bbv12I GWGCWC 1 cut(s) 318
BccI CCATC 1 cut(s) 329
BcgI CGANNNNNNTGC 2 cut(s) 258, 292
BcoDI GTCTC 3 cut(s) 114, 155, 443
BfrI CTTAAG 1 cut(s) 431
BfuAI ACCTGC 1 cut(s) 31
Bme18I GGWCC 1 cut(s) 20
BmgT120I GGNCC 1 cut(s) 20
BpiI GAAGAC 1 cut(s) 388
BpmI CTGGAG 1 cut(s) 120
BsaJI CCNNGG 1 cut(s) 413
BsaWI WCCGGW 1 cut(s) 61
Bsc4I CCNNNNNNNGG 2 cut(s) 327, 419
Bse21I CCTNAGG 1 cut(s) 261
BseDI CCNNGG 1 cut(s) 413
BseGI GGATG 1 cut(s) 190
BseLI CCNNNNNNNGG 2 cut(s) 327, 419
BseMII CTCAG 1 cut(s) 252
BseRI GAGGAG 1 cut(s) 310
BsiHKAI GWGCWC 1 cut(s) 318
BsiSI CCGG 1 cut(s) 62
BslI CCNNNNNNNGG 2 cut(s) 327, 419
BsmAI GTCTC 3 cut(s) 114, 155, 443
Bsp1286I GDGCHC 1 cut(s) 318
Bsp143I GATC 2 cut(s) 187, 405
Bsp19I CCATGG 1 cut(s) 413
BspCNI CTCAG 1 cut(s) 253
BspHI TCATGA 1 cut(s) 33
BspMI ACCTGC 1 cut(s) 31
BspPI GGATC 1 cut(s) 413
BspTI CTTAAG 1 cut(s) 431
BssECI CCNNGG 1 cut(s) 413
BssMI GATC 2 cut(s) 187, 405
BssT1I CCWWGG 1 cut(s) 413
Bst4CI ACNGT 3 cut(s) 169, 221, 400
Bst6I CTCTTC 1 cut(s) 112
BstAFI CTTAAG 1 cut(s) 431
BstC8I GCNNGC 2 cut(s) 153, 363
BstDEI CTNAG 2 cut(s) 261, 440
BstDSI CCRYGG 1 cut(s) 413
BstF5I GGATG 1 cut(s) 190
BstKTI GATC 2 cut(s) 190, 408
BstMAI GTCTC 3 cut(s) 114, 155, 443
BstMBI GATC 2 cut(s) 187, 405
BstMWI GCNNNNNNNGC 1 cut(s) 148
BstV2I GAAGAC 1 cut(s) 388
Bsu36I CCTNAGG 1 cut(s) 261
BtgI CCRYGG 1 cut(s) 413
BtsCI GGATG 1 cut(s) 190
BtsI GCAGTG 1 cut(s) 318
BtsIMutI CAGTG 2 cut(s) 318, 405
BveI ACCTGC 1 cut(s) 31
Cac8I GCNNGC 2 cut(s) 153, 363
CciI TCATGA 1 cut(s) 33
Cfr13I GGNCC 1 cut(s) 20
CviAII CATG 4 cut(s) 34, 158, 327, 414
CviJI RGCY 5 cut(s) 55, 175, 289, 339, 412
CviKI_1 RGCY 5 cut(s) 55, 175, 289, 339, 412
DdeI CTNAG 2 cut(s) 261, 440
DpnI GATC 2 cut(s) 189, 407
DpnII GATC 2 cut(s) 187, 405
DraIII CACNNNGTG 1 cut(s) 402
Eam1104I CTCTTC 1 cut(s) 112
EarI CTCTTC 1 cut(s) 112
Eco130I CCWWGG 1 cut(s) 413
Eco47I GGWCC 1 cut(s) 20
Eco57I CTGAAG 1 cut(s) 258
Eco81I CCTNAGG 1 cut(s) 261
EcoT14I CCWWGG 1 cut(s) 413
ErhI CCWWGG 1 cut(s) 413
FaeI CATG 4 cut(s) 37, 161, 330, 417
FalI AAGNNNNNCTT 2 cut(s) 278, 310
FatI CATG 4 cut(s) 33, 157, 326, 413
FokI GGATG 1 cut(s) 197
GsuI CTGGAG 1 cut(s) 120
HapII CCGG 1 cut(s) 62
Hin1II CATG 4 cut(s) 37, 161, 330, 417
HindIII AAGCTT 2 cut(s) 173, 287
HinfI GANTC 2 cut(s) 212, 436
HpaII CCGG 1 cut(s) 62
Hpy166II GTNNAC 1 cut(s) 20
Hpy188I TCNGA 2 cut(s) 14, 443
Hpy188III TCNNGA 4 cut(s) 34, 137, 236, 260
Hpy8I GTNNAC 1 cut(s) 20
HpyAV CCTTC 2 cut(s) 197, 233
HpyCH4III ACNGT 3 cut(s) 169, 221, 400
HpyCH4IV ACGT 1 cut(s) 48
HpyCH4V TGCA 3 cut(s) 42, 361, 428
HpyF10VI GCNNNNNNNGC 1 cut(s) 148
HpyF3I CTNAG 2 cut(s) 261, 440
HpySE526I ACGT 1 cut(s) 48
Hsp92II CATG 4 cut(s) 37, 161, 330, 417
Kzo9I GATC 2 cut(s) 187, 405
LmnI GCTCC 1 cut(s) 303
LpnPI CCDG 7 cut(s) 12, 36, 74, 75, 150, 249, 273
MaeII ACGT 1 cut(s) 48
MalI GATC 2 cut(s) 189, 407
MboI GATC 2 cut(s) 187, 405
MboII GAAGA 2 cut(s) 129, 393
MhlI GDGCHC 1 cut(s) 318
MluCI AATT 1 cut(s) 298
MlyI GAGTC 2 cut(s) 206, 445
MnlI CCTC 4 cut(s) 8, 256, 331, 340
MseI TTAA 2 cut(s) 147, 432
MspCI CTTAAG 1 cut(s) 431
MspI CCGG 1 cut(s) 62
MwoI GCNNNNNNNGC 1 cut(s) 148
NcoI CCATGG 1 cut(s) 413
NdeII GATC 2 cut(s) 187, 405
NlaIII CATG 4 cut(s) 37, 161, 330, 417
PagI TCATGA 1 cut(s) 33
PflMI CCANNNNNTGG 1 cut(s) 419
PleI GAGTC 2 cut(s) 206, 444
PpsI GAGTC 2 cut(s) 206, 444
PspPI GGNCC 1 cut(s) 20
SaqAI TTAA 2 cut(s) 147, 432
Sau3AI GATC 2 cut(s) 187, 405
Sau96I GGNCC 1 cut(s) 20
SchI GAGTC 2 cut(s) 206, 445
SduI GDGCHC 1 cut(s) 318
SetI ASST 7 cut(s) 19, 25, 51, 57, 177, 244, 291
SinI GGWCC 1 cut(s) 20
SmlI CTYRAG 1 cut(s) 431
SmoI CTYRAG 1 cut(s) 431
Sse9I AATT 1 cut(s) 298
StyI CCWWGG 1 cut(s) 413
TaaI ACNGT 3 cut(s) 169, 221, 400
TaiI ACGT 1 cut(s) 51
TaqI TCGA 1 cut(s) 278
TasI AATT 1 cut(s) 298
Tru1I TTAA 2 cut(s) 147, 432
Tru9I TTAA 2 cut(s) 147, 432
TscAI CASTG 2 cut(s) 318, 405
TspDTI ATGAA 3 cut(s) 50, 244, 387
TspRI CASTG 2 cut(s) 318, 405
Van91I CCANNNNNTGG 1 cut(s) 419
Vha464I CTTAAG 1 cut(s) 431
VpaK11BI GGWCC 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.