Catalytic subunit of pyrophosphate--fructose 6-phosphate 1-phosphotransferase. Catalyzes the phosphorylation of D-fructose 6-phosphate, the first committing step of glycolysis. Uses inorganic phosphate (PPi) as phosphoryl donor instead of ATP like common ATP-dependent phosphofructokinases (ATP-PFKs), which renders the reaction reversible, and can thus function both in glycolysis and gluconeogenesis
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 259 |
| Acc36I | ACCTGC | 2 cut(s) | 186, 259 |
| AccB1I | GGYRCC | 2 cut(s) | 69, 363 |
| AccII | CGCG | 5 cut(s) | 67, 145, 333, 345, 768 |
| AclWI | GGATC | 2 cut(s) | 626, 639 |
| AcoI | YGGCCR | 2 cut(s) | 201, 676 |
| AcyI | GRCGYC | 1 cut(s) | 70 |
| AfaI | GTAC | 2 cut(s) | 125, 653 |
| AfeI | AGCGCT | 1 cut(s) | 824 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 16, 168, 572 |
| AgsI | TTSAA | 2 cut(s) | 509, 833 |
| AjnI | CCWGG | 1 cut(s) | 815 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 501, 533 |
| AluBI | AGCT | 8 cut(s) | 188, 224, 393, 469, 517, 557, 795, 802 |
| AluI | AGCT | 8 cut(s) | 188, 224, 393, 469, 517, 557, 795, 802 |
| Alw21I | GWGCWC | 2 cut(s) | 476, 804 |
| Alw26I | GTCTC | 1 cut(s) | 182 |
| Alw44I | GTGCAC | 1 cut(s) | 472 |
| AlwI | GGATC | 2 cut(s) | 626, 639 |
| Aor51HI | AGCGCT | 1 cut(s) | 824 |
| AoxI | GGCC | 5 cut(s) | 201, 279, 676, 708, 813 |
| ApaI | GGGCCC | 1 cut(s) | 283 |
| ApaLI | GTGCAC | 1 cut(s) | 472 |
| ApeKI | GCWGC | 2 cut(s) | 387, 390 |
| AspLEI | GCGC | 5 cut(s) | 72, 147, 333, 347, 825 |
| AspS9I | GGNCC | 3 cut(s) | 279, 280, 813 |
| AsuHPI | GGTGA | 1 cut(s) | 278 |
| AxyI | CCTNAGG | 1 cut(s) | 809 |
| BaeGI | GKGCMC | 2 cut(s) | 283, 476 |
| BamHI | GGATCC | 1 cut(s) | 631 |
| BanI | GGYRCC | 2 cut(s) | 69, 363 |
| BanII | GRGCYC | 2 cut(s) | 283, 804 |
| BbsI | GAAGAC | 1 cut(s) | 840 |
| Bbv12I | GWGCWC | 2 cut(s) | 476, 804 |
| BbvI | GCAGC | 2 cut(s) | 399, 402 |
| BccI | CCATC | 1 cut(s) | 605 |
| BceAI | ACGGC | 1 cut(s) | 310 |
| BciT130I | CCWGG | 1 cut(s) | 817 |
| BcoDI | GTCTC | 1 cut(s) | 182 |
| BfaI | CTAG | 1 cut(s) | 749 |
| BfmI | CTRYAG | 1 cut(s) | 193 |
| BfoI | RGCGCY | 2 cut(s) | 73, 826 |
| BfuAI | ACCTGC | 2 cut(s) | 186, 259 |
| BmcAI | AGTACT | 1 cut(s) | 653 |
| Bme1390I | CCNGG | 1 cut(s) | 817 |
| BmgT120I | GGNCC | 3 cut(s) | 279, 280, 813 |
| BmiI | GGNNCC | 5 cut(s) | 71, 281, 365, 551, 633 |
| BmrFI | CCNGG | 1 cut(s) | 817 |
| BmsI | GCATC | 1 cut(s) | 579 |
| BpiI | GAAGAC | 1 cut(s) | 840 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 73, 105 |
| BpmI | CTGGAG | 2 cut(s) | 145, 353 |
| BsaHI | GRCGYC | 1 cut(s) | 70 |
| BsaI | GGTCTC | 1 cut(s) | 182 |
| BsaJI | CCNNGG | 3 cut(s) | 65, 416, 679 |
| BsaWI | WCCGGW | 1 cut(s) | 91 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 16, 168, 572 |
| Bse118I | RCCGGY | 1 cut(s) | 203 |
| Bse21I | CCTNAGG | 1 cut(s) | 809 |
| BseBI | CCWGG | 1 cut(s) | 817 |
| BseDI | CCNNGG | 3 cut(s) | 65, 416, 679 |
| BseGI | GGATG | 2 cut(s) | 84, 616 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 16, 168, 572 |
| BseMII | CTCAG | 3 cut(s) | 193, 467, 803 |
| BseRI | GAGGAG | 2 cut(s) | 231, 234 |
| BseSI | GKGCMC | 2 cut(s) | 283, 476 |
| BseX3I | CGGCCG | 1 cut(s) | 201 |
| BseXI | GCAGC | 2 cut(s) | 399, 402 |
| BseYI | CCCAGC | 1 cut(s) | 224 |
| Bsh1236I | CGCG | 5 cut(s) | 67, 145, 333, 345, 768 |
| Bsh1285I | CGRYCG | 2 cut(s) | 48, 204 |
| BshFI | GGCC | 5 cut(s) | 203, 281, 678, 710, 815 |
| BshNI | GGYRCC | 2 cut(s) | 69, 363 |
| BsiEI | CGRYCG | 2 cut(s) | 48, 204 |
| BsiHKAI | GWGCWC | 2 cut(s) | 476, 804 |
| BsiSI | CCGG | 3 cut(s) | 76, 92, 204 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 16, 168, 572 |
| BsmAI | GTCTC | 1 cut(s) | 182 |
| BsnI | GGCC | 5 cut(s) | 203, 281, 678, 710, 815 |
| Bso31I | GGTCTC | 1 cut(s) | 182 |
| Bsp120I | GGGCCC | 1 cut(s) | 279 |
| Bsp1286I | GDGCHC | 3 cut(s) | 283, 476, 804 |
| Bsp143I | GATC | 2 cut(s) | 154, 631 |
| BspANI | GGCC | 5 cut(s) | 203, 281, 678, 710, 815 |
| BspCNI | CTCAG | 3 cut(s) | 192, 466, 802 |
| BspFNI | CGCG | 5 cut(s) | 67, 145, 333, 345, 768 |
| BspLI | GGNNCC | 5 cut(s) | 71, 281, 365, 551, 633 |
| BspMAI | CTGCAG | 1 cut(s) | 197 |
| BspMI | ACCTGC | 2 cut(s) | 186, 259 |
| BspPI | GGATC | 2 cut(s) | 626, 639 |
| BspT107I | GGYRCC | 2 cut(s) | 69, 363 |
| BspTNI | GGTCTC | 1 cut(s) | 182 |
| BsrFI | RCCGGY | 1 cut(s) | 203 |
| BssAI | RCCGGY | 1 cut(s) | 203 |
| BssECI | CCNNGG | 3 cut(s) | 65, 416, 679 |
| BssMI | GATC | 2 cut(s) | 154, 631 |
| BssNI | GRCGYC | 1 cut(s) | 70 |
| BssT1I | CCWWGG | 2 cut(s) | 416, 679 |
| Bst2UI | CCWGG | 1 cut(s) | 817 |
| Bst4CI | ACNGT | 1 cut(s) | 656 |
| Bst6I | CTCTTC | 1 cut(s) | 237 |
| BstACI | GRCGYC | 1 cut(s) | 70 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 117 |
| BstC8I | GCNNGC | 2 cut(s) | 205, 617 |
| BstDEI | CTNAG | 4 cut(s) | 179, 453, 789, 809 |
| BstDSI | CCRYGG | 1 cut(s) | 65 |
| BstF5I | GGATG | 2 cut(s) | 84, 616 |
| BstFNI | CGCG | 5 cut(s) | 67, 145, 333, 345, 768 |
| BstH2I | RGCGCY | 2 cut(s) | 73, 826 |
| BstHHI | GCGC | 5 cut(s) | 72, 147, 333, 347, 825 |
| BstKTI | GATC | 2 cut(s) | 157, 634 |
| BstMAI | GTCTC | 1 cut(s) | 182 |
| BstMBI | GATC | 2 cut(s) | 154, 631 |
| BstMCI | CGRYCG | 2 cut(s) | 48, 204 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 117, 370, 784 |
| BstNI | CCWGG | 1 cut(s) | 817 |
| BstSCI | CCNGG | 1 cut(s) | 815 |
| BstSFI | CTRYAG | 1 cut(s) | 193 |
| BstSLI | GKGCMC | 2 cut(s) | 283, 476 |
| BstUI | CGCG | 5 cut(s) | 67, 145, 333, 345, 768 |
| BstV1I | GCAGC | 2 cut(s) | 399, 402 |
| BstV2I | GAAGAC | 1 cut(s) | 840 |
| BstX2I | RGATCY | 1 cut(s) | 631 |
| BstYI | RGATCY | 1 cut(s) | 631 |
| BstZI | CGGCCG | 1 cut(s) | 201 |
| Bsu36I | CCTNAGG | 1 cut(s) | 809 |
| BsuRI | GGCC | 5 cut(s) | 203, 281, 678, 710, 815 |
| BtgI | CCRYGG | 1 cut(s) | 65 |
| BtsCI | GGATG | 2 cut(s) | 84, 616 |
| BtsI | GCAGTG | 1 cut(s) | 651 |
| BtsIMutI | CAGTG | 2 cut(s) | 505, 651 |
| BveI | ACCTGC | 2 cut(s) | 186, 259 |
| Cac8I | GCNNGC | 2 cut(s) | 205, 617 |
| CfoI | GCGC | 5 cut(s) | 72, 147, 333, 347, 825 |
| Cfr10I | RCCGGY | 1 cut(s) | 203 |
| Cfr13I | GGNCC | 3 cut(s) | 279, 280, 813 |
| Cfr42I | CCGCGG | 1 cut(s) | 68 |
| Csp6I | GTAC | 2 cut(s) | 124, 652 |
| CviQI | GTAC | 2 cut(s) | 124, 652 |
| DdeI | CTNAG | 4 cut(s) | 179, 453, 789, 809 |
| DinI | GGCGCC | 1 cut(s) | 71 |
| DpnI | GATC | 2 cut(s) | 156, 633 |
| DpnII | GATC | 2 cut(s) | 154, 631 |
| EaeI | YGGCCR | 2 cut(s) | 201, 676 |
| EagI | CGGCCG | 1 cut(s) | 201 |
| Eam1104I | CTCTTC | 1 cut(s) | 237 |
| EarI | CTCTTC | 1 cut(s) | 237 |
| EciI | GGCGGA | 1 cut(s) | 228 |
| Ecl136II | GAGCTC | 1 cut(s) | 802 |
| EclXI | CGGCCG | 1 cut(s) | 201 |
| Eco130I | CCWWGG | 2 cut(s) | 416, 679 |
| Eco147I | AGGCCT | 1 cut(s) | 710 |
| Eco24I | GRGCYC | 2 cut(s) | 283, 804 |
| Eco31I | GGTCTC | 1 cut(s) | 182 |
| Eco47III | AGCGCT | 1 cut(s) | 824 |
| Eco52I | CGGCCG | 1 cut(s) | 201 |
| Eco53kI | GAGCTC | 1 cut(s) | 802 |
| Eco81I | CCTNAGG | 1 cut(s) | 809 |
| EcoICRI | GAGCTC | 1 cut(s) | 802 |
| EcoO109I | RGGNCCY | 2 cut(s) | 279, 280 |
| EcoRII | CCWGG | 1 cut(s) | 815 |
| EcoT14I | CCWWGG | 2 cut(s) | 416, 679 |
| EcoT38I | GRGCYC | 2 cut(s) | 283, 804 |
| EgeI | GGCGCC | 1 cut(s) | 71 |
| EheI | GGCGCC | 1 cut(s) | 71 |
| ErhI | CCWWGG | 2 cut(s) | 416, 679 |
| FaiI | YATR | 5 cut(s) | 32, 356, 396, 442, 444 |
| FokI | GGATG | 2 cut(s) | 91, 623 |
| FriOI | GRGCYC | 2 cut(s) | 283, 804 |
| FspBI | CTAG | 1 cut(s) | 749 |
| GlaI | GCGC | 5 cut(s) | 71, 146, 332, 346, 824 |
| GsaI | CCCAGC | 1 cut(s) | 228 |
| GsuI | CTGGAG | 2 cut(s) | 145, 353 |
| HaeII | RGCGCY | 2 cut(s) | 73, 826 |
| HaeIII | GGCC | 5 cut(s) | 203, 281, 678, 710, 815 |
| HapII | CCGG | 3 cut(s) | 76, 92, 204 |
| HhaI | GCGC | 5 cut(s) | 72, 147, 333, 347, 825 |
| Hin1I | GRCGYC | 1 cut(s) | 70 |
| Hin6I | GCGC | 5 cut(s) | 70, 145, 331, 345, 823 |
| HinP1I | GCGC | 5 cut(s) | 70, 145, 331, 345, 823 |
| HindIII | AAGCTT | 1 cut(s) | 186 |
| HinfI | GANTC | 2 cut(s) | 5, 248 |
| HpaII | CCGG | 3 cut(s) | 76, 92, 204 |
| HphI | GGTGA | 1 cut(s) | 278 |
| Hpy166II | GTNNAC | 1 cut(s) | 474 |
| Hpy188I | TCNGA | 7 cut(s) | 45, 137, 182, 253, 316, 456, 792 |
| Hpy188III | TCNNGA | 4 cut(s) | 152, 162, 236, 586 |
| Hpy8I | GTNNAC | 1 cut(s) | 474 |
| Hpy99I | CGWCG | 4 cut(s) | 257, 260, 296, 768 |
| HpyAV | CCTTC | 5 cut(s) | 159, 293, 315, 388, 683 |
| HpyCH4III | ACNGT | 1 cut(s) | 656 |
| HpyCH4V | TGCA | 4 cut(s) | 195, 474, 667, 698 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 117, 370, 784 |
| HpyF3I | CTNAG | 4 cut(s) | 179, 453, 789, 809 |
| Hsp92I | GRCGYC | 1 cut(s) | 70 |
| HspAI | GCGC | 5 cut(s) | 70, 145, 331, 345, 823 |
| KasI | GGCGCC | 1 cut(s) | 69 |
| KroI | GCCGGC | 1 cut(s) | 203 |
| KroNI | GCCGGC | 1 cut(s) | 205 |
| KspI | CCGCGG | 1 cut(s) | 68 |
| Kzo9I | GATC | 2 cut(s) | 154, 631 |
| LmnI | GCTCC | 4 cut(s) | 221, 514, 799, 830 |
| Lsp1109I | GCAGC | 2 cut(s) | 399, 402 |
| LweI | GCATC | 1 cut(s) | 579 |
| MaeI | CTAG | 1 cut(s) | 749 |
| MaeIII | GTNAC | 1 cut(s) | 575 |
| MalI | GATC | 2 cut(s) | 156, 633 |
| MboI | GATC | 2 cut(s) | 154, 631 |
| MboII | GAAGA | 4 cut(s) | 254, 501, 783, 845 |
| MfeI | CAATTG | 1 cut(s) | 524 |
| MflI | RGATCY | 1 cut(s) | 631 |
| MhlI | GDGCHC | 3 cut(s) | 283, 476, 804 |
| MluCI | AATT | 1 cut(s) | 524 |
| Mly113I | GGCGCC | 1 cut(s) | 70 |
| MmeI | TCCRAC | 3 cut(s) | 68, 276, 384 |
| MroNI | GCCGGC | 1 cut(s) | 203 |
| MseI | TTAA | 1 cut(s) | 449 |
| MspA1I | CMGCKG | 2 cut(s) | 67, 469 |
| MspI | CCGG | 3 cut(s) | 76, 92, 204 |
| MspR9I | CCNGG | 1 cut(s) | 817 |
| MunI | CAATTG | 1 cut(s) | 524 |
| MvaI | CCWGG | 1 cut(s) | 817 |
| MvnI | CGCG | 5 cut(s) | 67, 145, 333, 345, 768 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 117, 370, 784 |
| NaeI | GCCGGC | 1 cut(s) | 205 |
| NarI | GGCGCC | 1 cut(s) | 70 |
| NdeII | GATC | 2 cut(s) | 154, 631 |
| NgoMIV | GCCGGC | 1 cut(s) | 203 |
| NlaIV | GGNNCC | 5 cut(s) | 71, 281, 365, 551, 633 |
| PaqCI | CACCTGC | 1 cut(s) | 259 |
| PceI | AGGCCT | 1 cut(s) | 710 |
| PdiI | GCCGGC | 1 cut(s) | 205 |
| PfeI | GAWTC | 2 cut(s) | 5, 248 |
| PluTI | GGCGCC | 1 cut(s) | 73 |
| Psp124BI | GAGCTC | 1 cut(s) | 804 |
| Psp6I | CCWGG | 1 cut(s) | 815 |
| PspFI | CCCAGC | 1 cut(s) | 224 |
| PspGI | CCWGG | 1 cut(s) | 815 |
| PspN4I | GGNNCC | 5 cut(s) | 71, 281, 365, 551, 633 |
| PspOMI | GGGCCC | 1 cut(s) | 279 |
| PspPI | GGNCC | 3 cut(s) | 279, 280, 813 |
| PstI | CTGCAG | 1 cut(s) | 197 |
| PsuI | RGATCY | 1 cut(s) | 631 |
| PvuII | CAGCTG | 1 cut(s) | 469 |
| RsaI | GTAC | 2 cut(s) | 125, 653 |
| RsaNI | GTAC | 2 cut(s) | 124, 652 |
| SacI | GAGCTC | 1 cut(s) | 804 |
| SacII | CCGCGG | 1 cut(s) | 68 |
| SaqAI | TTAA | 1 cut(s) | 449 |
| Sau3AI | GATC | 2 cut(s) | 154, 631 |
| Sau96I | GGNCC | 3 cut(s) | 279, 280, 813 |
| SbfI | CCTGCAGG | 1 cut(s) | 197 |
| ScaI | AGTACT | 1 cut(s) | 653 |
| ScrFI | CCNGG | 1 cut(s) | 817 |
| SdaI | CCTGCAGG | 1 cut(s) | 197 |
| SduI | GDGCHC | 3 cut(s) | 283, 476, 804 |
| SfaNI | GCATC | 1 cut(s) | 579 |
| SfcI | CTRYAG | 1 cut(s) | 193 |
| SfoI | GGCGCC | 1 cut(s) | 71 |
| Sfr303I | CCGCGG | 1 cut(s) | 68 |
| SgrBI | CCGCGG | 1 cut(s) | 68 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 197 |
| Sse9I | AATT | 1 cut(s) | 524 |
| SseBI | AGGCCT | 1 cut(s) | 710 |
| SspDI | GGCGCC | 1 cut(s) | 69 |
| SspMI | CTAG | 1 cut(s) | 749 |
| SstI | GAGCTC | 1 cut(s) | 804 |
| StuI | AGGCCT | 1 cut(s) | 710 |
| StyD4I | CCNGG | 1 cut(s) | 815 |
| StyI | CCWWGG | 2 cut(s) | 416, 679 |
| TaaI | ACNGT | 1 cut(s) | 656 |
| TaqI | TCGA | 3 cut(s) | 291, 381, 627 |
| TaqII | GACCGA | 1 cut(s) | 164 |
| TasI | AATT | 1 cut(s) | 524 |
| TatI | WGTACW | 2 cut(s) | 123, 651 |
| TauI | GCSGC | 8 cut(s) | 70, 75, 145, 210, 213, 361, 364, 678 |
| TfiI | GAWTC | 2 cut(s) | 5, 248 |
| Tru1I | TTAA | 1 cut(s) | 449 |
| Tru9I | TTAA | 1 cut(s) | 449 |
| TscAI | CASTG | 2 cut(s) | 505, 651 |
| TseI | GCWGC | 2 cut(s) | 387, 390 |
| TspDTI | ATGAA | 2 cut(s) | 494, 654 |
| TspGWI | ACGGA | 1 cut(s) | 50 |
| TspRI | CASTG | 2 cut(s) | 505, 651 |
| VneI | GTGCAC | 1 cut(s) | 472 |
| XspI | CTAG | 1 cut(s) | 749 |
| ZrmI | AGTACT | 1 cut(s) | 653 |