pycom05g32020

Transcriptional repressor, ovate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0011981
Physical Location & Seq
Forward (+)
31552459 .. 31553750
1292 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g32020.6

Sequence Viewer

Length: 1050 bp
ATGCGTTTCTTTTTCTTCAACAAGAAACACTTCAAAGGTTCTTCTAATCAAGCGCTTACGAAGATAAGTACTTCCTACAAAAGCAATCCCATACGGAGCCTTCCAAACAAGCTCTCATGGAATAGAACCACTACTTTTCATGTTAACCAAACTCCTCAAAAGGATGTGCCAACTAACATACTGGCTCGATTAACTAAAAGAATGTTCCCCATTTGCCTTCTTGCATTACCAACTCATCTATTAAAATGTTATAAAACACAAGACACAATCATGATTAACAAACCCCACAAATCTCCAATTCAAACCATGTCTAAAACGAAAGCCTCCTCCAAGAAGCAACGGAGAATATGCAAGGCATTGTGCTGCAGCAGCTGCCGGCTTAGTGTGTCTTCGTCTAGCTCGGAGGAAGTAGCCAGCTCTAGTTCCGATAGGTACCCTTCAATATCAAGCCTATCGCATGCCATGGTTCAAGAGAGACTCGACCAGATGATCAGGGAAGGGCAGGAGGGCTCGAGGCACGTTGATCATCGGAGAAGAAAGCAGCGAGCTGGTGATGATCATGATCAACTTCAAGCAGGAGGAGGGACTAAATTTGTTGTAATGGTTGCGATGGAGATGTGTTCTTATGATCCGAGGGAGGATTTCAGGGAGTCCATGGCGGCGATGATAGTGGCAAACCGAATACAAGAGCCTAAAGATCTTCGTAGCCTCTTGAATTATTATGTTTCGATGAATTCAGATGAGTATCGTGGGCTTATACTTGAGGTGTTTCATGAAGTTTGCTCTGATTTTTTTCTTATCCAACTATGGAAAAGCAGCTTTGAACTTGATTTAAATTTAAGACAAAAACAAAATTGTGACATTATATTGGGGAGAAAAGAAGACGAACCCATATGTAATATTCAAATGATAACTACAAATCTAACAATAACTTATGTTGCAAAATTTTTGAATCTGCACCACTTATTCTGCCGCGACGAAAGTTTATGTGATTCTTGCCCCTTATTGATCTCCTCTTGGGAAGGCCAAGAAAGCGGAGTAAGGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ovate

Protein Analysis

350

Amino Acids

40.14

Weight (kDa)

9.18

Isoelectric Point (pI)

53.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011981)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G04025
fragaria_vesca FvH4_3g01350
malus_domestica MD05G1352900.v1.1 MD10G1327500.v1.1
prunus_persica Prupe.4G014500_v2.0.a1
pyrus_communis pycom05g32020 pycom05g32100 pycom17g07250
rosa_chinensis RchiOBHm_Chr5g0001971
rosa_laevigata RLG00000030985
rosa_multiflora Rmu_sc0000378.1_g000017 Rmu_sc0006008.1_g000006
rosa_roxburghii Rroxscaffold_1G00071360 Rroxscaffold_1G00074380
rosa_rugosa Rorug04G0394100
rosa_samantha Rh5BG018800 Rh5CG016500 Rh5DG017100
rosa_wichuraiana Rw5G001490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 252
Acc65I GGTACC 1 cut(s) 432
AccB1I GGYRCC 1 cut(s) 432
AccII CGCG 1 cut(s) 975
AciI CCGC 3 cut(s) 659, 973, 1035
AclWI GGATC 1 cut(s) 623
AcsI RAATTY 4 cut(s) 590, 733, 835, 944
AfaI GTAC 2 cut(s) 70, 434
AfeI AGCGCT 1 cut(s) 54
AluBI AGCT 6 cut(s) 112, 372, 399, 417, 548, 819
AluI AGCT 6 cut(s) 112, 372, 399, 417, 548, 819
Alw26I GTCTC 1 cut(s) 469
AlwI GGATC 1 cut(s) 623
AlwNI CAGNNNCTG 1 cut(s) 372
Ama87I CYCGRG 1 cut(s) 511
Aor51HI AGCGCT 1 cut(s) 54
AoxI GGCC 1 cut(s) 1024
ApeKI GCWGC 6 cut(s) 363, 366, 369, 372, 541, 816
ApoI RAATTY 4 cut(s) 590, 733, 835, 944
Asp700I GAANNNNTTC 1 cut(s) 29
Asp718I GGTACC 1 cut(s) 432
AspLEI GCGC 1 cut(s) 55
AsuHPI GGTGA 1 cut(s) 563
AvaI CYCGRG 1 cut(s) 511
BanI GGYRCC 1 cut(s) 432
BanII GRGCYC 1 cut(s) 512
BbsI GAAGAC 2 cut(s) 381, 888
BbvI GCAGC 6 cut(s) 350, 359, 378, 381, 553, 828
BccI CCATC 1 cut(s) 604
BclI TGATCA 4 cut(s) 489, 523, 556, 562
BcoDI GTCTC 1 cut(s) 469
BfaI CTAG 2 cut(s) 396, 420
BfmI CTRYAG 1 cut(s) 364
BfoI RGCGCY 1 cut(s) 56
BglII AGATCT 1 cut(s) 697
BisI GCNGC 8 cut(s) 364, 367, 370, 373, 542, 660, 817, 973
BlsI GCNGC 8 cut(s) 365, 368, 371, 374, 543, 661, 818, 974
BmcAI AGTACT 1 cut(s) 70
BmeT110I CYCGRG 1 cut(s) 511
BmiI GGNNCC 2 cut(s) 98, 434
BpiI GAAGAC 2 cut(s) 381, 888
BpuEI CTTGAG 1 cut(s) 782
BsaBI GATNNNNATC 2 cut(s) 561, 744
BsaJI CCNNGG 3 cut(s) 462, 632, 654
Bse118I RCCGGY 1 cut(s) 375
Bse1I ACTGG 1 cut(s) 186
Bse8I GATNNNNATC 2 cut(s) 561, 744
BseDI CCNNGG 3 cut(s) 462, 632, 654
BseGI GGATG 1 cut(s) 169
BseJI GATNNNNATC 2 cut(s) 561, 744
BseNI ACTGG 1 cut(s) 186
BseRI GAGGAG 4 cut(s) 144, 316, 594, 1003
BseXI GCAGC 6 cut(s) 350, 359, 378, 381, 553, 828
BsgI GTGCAG 1 cut(s) 941
Bsh1236I CGCG 1 cut(s) 975
BshFI GGCC 1 cut(s) 1026
BshNI GGYRCC 1 cut(s) 432
BsiHKCI CYCGRG 1 cut(s) 511
BsiSI CCGG 1 cut(s) 376
BslFI GGGAC 1 cut(s) 598
BsmAI GTCTC 1 cut(s) 469
BsmFI GGGAC 1 cut(s) 598
BsnI GGCC 1 cut(s) 1026
BsoBI CYCGRG 1 cut(s) 511
Bsp1286I GDGCHC 1 cut(s) 512
Bsp143I GATC 7 cut(s) 489, 523, 556, 562, 628, 697, 1008
Bsp19I CCATGG 2 cut(s) 462, 654
BspACI CCGC 3 cut(s) 659, 973, 1035
BspANI GGCC 1 cut(s) 1026
BspFNI CGCG 1 cut(s) 975
BspHI TCATGA 3 cut(s) 270, 559, 772
BspLI GGNNCC 2 cut(s) 98, 434
BspMAI CTGCAG 1 cut(s) 368
BspPI GGATC 1 cut(s) 623
BspT107I GGYRCC 1 cut(s) 432
BsrFI RCCGGY 1 cut(s) 375
BsrI ACTGG 1 cut(s) 186
BssAI RCCGGY 1 cut(s) 375
BssECI CCNNGG 3 cut(s) 462, 632, 654
BssMI GATC 7 cut(s) 489, 523, 556, 562, 628, 697, 1008
BssT1I CCWWGG 2 cut(s) 462, 654
BstAPI GCANNNNNTGC 1 cut(s) 372
BstC8I GCNNGC 4 cut(s) 377, 415, 459, 546
BstDEI CTNAG 1 cut(s) 380
BstDSI CCRYGG 2 cut(s) 462, 654
BstF5I GGATG 1 cut(s) 169
BstFNI CGCG 1 cut(s) 975
BstH2I RGCGCY 1 cut(s) 56
BstHHI GCGC 1 cut(s) 55
BstKTI GATC 7 cut(s) 492, 526, 559, 565, 631, 700, 1011
BstMAI GTCTC 1 cut(s) 469
BstMBI GATC 7 cut(s) 489, 523, 556, 562, 628, 697, 1008
BstMWI GCNNNNNNNGC 3 cut(s) 369, 372, 1032
BstNSI RCATGY 1 cut(s) 461
BstSFI CTRYAG 1 cut(s) 364
BstUI CGCG 1 cut(s) 975
BstV1I GCAGC 6 cut(s) 350, 359, 378, 381, 553, 828
BstV2I GAAGAC 2 cut(s) 381, 888
BstX2I RGATCY 1 cut(s) 697
BstYI RGATCY 1 cut(s) 697
BsuRI GGCC 1 cut(s) 1026
BtgI CCRYGG 2 cut(s) 462, 654
BtgZI GCGATG 2 cut(s) 623, 677
BtsCI GGATG 1 cut(s) 169
Cac8I GCNNGC 4 cut(s) 377, 415, 459, 546
CaiI CAGNNNCTG 1 cut(s) 372
CciI TCATGA 3 cut(s) 270, 559, 772
CfoI GCGC 1 cut(s) 55
Cfr10I RCCGGY 1 cut(s) 375
Csp6I GTAC 2 cut(s) 69, 433
CviAII CATG 9 cut(s) 117, 140, 271, 307, 458, 463, 560, 655, 773
CviQI GTAC 2 cut(s) 69, 433
DdeI CTNAG 1 cut(s) 380
DpnI GATC 7 cut(s) 491, 525, 558, 564, 630, 699, 1010
DpnII GATC 7 cut(s) 489, 523, 556, 562, 628, 697, 1008
DraI TTTAAA 1 cut(s) 834
Eco130I CCWWGG 2 cut(s) 462, 654
Eco24I GRGCYC 1 cut(s) 512
Eco47III AGCGCT 1 cut(s) 54
Eco88I CYCGRG 1 cut(s) 511
EcoRI GAATTC 1 cut(s) 733
EcoT14I CCWWGG 2 cut(s) 462, 654
EcoT38I GRGCYC 1 cut(s) 512
ErhI CCWWGG 2 cut(s) 462, 654
FaeI CATG 9 cut(s) 120, 143, 274, 310, 461, 466, 563, 658, 776
FalI AAGNNNNNCTT 2 cut(s) 14, 46
FaqI GGGAC 1 cut(s) 598
FatI CATG 9 cut(s) 116, 139, 270, 306, 457, 462, 559, 654, 772
FauNDI CATATG 1 cut(s) 893
FbaI TGATCA 4 cut(s) 489, 523, 556, 562
Fnu4HI GCNGC 8 cut(s) 364, 367, 370, 373, 542, 660, 817, 973
FokI GGATG 1 cut(s) 176
FriOI GRGCYC 1 cut(s) 512
Fsp4HI GCNGC 8 cut(s) 364, 367, 370, 373, 542, 660, 817, 973
FspBI CTAG 2 cut(s) 396, 420
GlaI GCGC 1 cut(s) 54
GluI GCNGC 8 cut(s) 364, 367, 370, 373, 542, 660, 817, 973
HaeII RGCGCY 1 cut(s) 56
HaeIII GGCC 1 cut(s) 1026
HapII CCGG 1 cut(s) 376
HhaI GCGC 1 cut(s) 55
Hin1II CATG 9 cut(s) 120, 143, 274, 310, 461, 466, 563, 658, 776
Hin6I GCGC 1 cut(s) 53
HinP1I GCGC 1 cut(s) 53
HincII GTYRAC 1 cut(s) 145
HindII GTYRAC 1 cut(s) 145
HinfI GANTC 4 cut(s) 477, 650, 952, 992
HpaI GTTAAC 1 cut(s) 145
HpaII CCGG 1 cut(s) 376
HphI GGTGA 1 cut(s) 563
Hpy166II GTNNAC 1 cut(s) 145
Hpy188I TCNGA 6 cut(s) 403, 427, 531, 633, 739, 787
Hpy188III TCNNGA 5 cut(s) 271, 470, 560, 712, 773
Hpy8I GTNNAC 1 cut(s) 145
Hpy99I CGWCG 1 cut(s) 980
HpyAV CCTTC 5 cut(s) 110, 227, 447, 491, 1016
HpyCH4IV ACGT 1 cut(s) 519
HpyCH4V TGCA 5 cut(s) 224, 351, 366, 941, 958
HpyF10VI GCNNNNNNNGC 3 cut(s) 369, 372, 1032
HpyF3I CTNAG 1 cut(s) 380
HpySE526I ACGT 1 cut(s) 519
Hsp92II CATG 9 cut(s) 120, 143, 274, 310, 461, 466, 563, 658, 776
HspAI GCGC 1 cut(s) 53
KpnI GGTACC 1 cut(s) 436
KroI GCCGGC 1 cut(s) 375
KroNI GCCGGC 1 cut(s) 377
Ksp22I TGATCA 4 cut(s) 489, 523, 556, 562
KspAI GTTAAC 1 cut(s) 145
Kzo9I GATC 7 cut(s) 489, 523, 556, 562, 628, 697, 1008
LmnI GCTCC 1 cut(s) 96
LpnPI CCDG 9 cut(s) 167, 389, 427, 478, 488, 497, 534, 561, 631
Lsp1109I GCAGC 6 cut(s) 350, 359, 378, 381, 553, 828
MaeI CTAG 2 cut(s) 396, 420
MaeII ACGT 1 cut(s) 519
MaeIII GTNAC 1 cut(s) 857
MalI GATC 7 cut(s) 491, 525, 558, 564, 630, 699, 1010
MboI GATC 7 cut(s) 489, 523, 556, 562, 628, 697, 1008
MboII GAAGA 7 cut(s) 7, 33, 73, 381, 546, 692, 893
MflI RGATCY 1 cut(s) 697
MhlI GDGCHC 1 cut(s) 512
MluCI AATT 7 cut(s) 297, 590, 715, 733, 835, 853, 944
MlyI GAGTC 2 cut(s) 471, 659
MmeI TCCRAC 1 cut(s) 826
MroNI GCCGGC 1 cut(s) 375
MroXI GAANNNNTTC 1 cut(s) 29
MseI TTAA 6 cut(s) 144, 191, 242, 276, 833, 839
MslI CAYNNNNRTG 1 cut(s) 269
MspA1I CMGCKG 1 cut(s) 372
MspI CCGG 1 cut(s) 376
MvnI CGCG 1 cut(s) 975
MwoI GCNNNNNNNGC 3 cut(s) 369, 372, 1032
NaeI GCCGGC 1 cut(s) 377
NcoI CCATGG 2 cut(s) 462, 654
NdeI CATATG 1 cut(s) 893
NdeII GATC 7 cut(s) 489, 523, 556, 562, 628, 697, 1008
NgoMIV GCCGGC 1 cut(s) 375
NlaIII CATG 9 cut(s) 120, 143, 274, 310, 461, 466, 563, 658, 776
NlaIV GGNNCC 2 cut(s) 98, 434
NmuCI GTSAC 1 cut(s) 857
NspI RCATGY 1 cut(s) 461
PaeI GCATGC 1 cut(s) 461
PaeR7I CTCGAG 1 cut(s) 511
PagI TCATGA 3 cut(s) 270, 559, 772
PdiI GCCGGC 1 cut(s) 377
PdmI GAANNNNTTC 1 cut(s) 29
PfeI GAWTC 2 cut(s) 952, 992
PkrI GCNGC 8 cut(s) 365, 368, 371, 374, 543, 661, 818, 974
PleI GAGTC 2 cut(s) 471, 658
PpsI GAGTC 2 cut(s) 471, 658
PsiI TTATAA 1 cut(s) 252
PspN4I GGNNCC 2 cut(s) 98, 434
PspXI VCTCGAGB 1 cut(s) 511
PstI CTGCAG 1 cut(s) 368
PstNI CAGNNNCTG 1 cut(s) 372
PsuI RGATCY 1 cut(s) 697
PvuII CAGCTG 1 cut(s) 372
RsaI GTAC 2 cut(s) 70, 434
RsaNI GTAC 2 cut(s) 69, 433
RseI CAYNNNNRTG 1 cut(s) 269
SaqAI TTAA 6 cut(s) 144, 191, 242, 276, 833, 839
SatI GCNGC 8 cut(s) 364, 367, 370, 373, 542, 660, 817, 973
Sau3AI GATC 7 cut(s) 489, 523, 556, 562, 628, 697, 1008
ScaI AGTACT 1 cut(s) 70
SchI GAGTC 2 cut(s) 471, 659
SduI GDGCHC 1 cut(s) 512
SfcI CTRYAG 1 cut(s) 364
Sfr274I CTCGAG 1 cut(s) 511
SlaI CTCGAG 1 cut(s) 511
SmiI ATTTAAAT 1 cut(s) 834
SmiMI CAYNNNNRTG 1 cut(s) 269
SmlI CTYRAG 2 cut(s) 511, 761
SmoI CTYRAG 2 cut(s) 511, 761
SphI GCATGC 1 cut(s) 461
Sse9I AATT 7 cut(s) 297, 590, 715, 733, 835, 853, 944
SsiI CCGC 3 cut(s) 659, 973, 1035
SspI AATATT 1 cut(s) 901
SspMI CTAG 2 cut(s) 396, 420
StyI CCWWGG 2 cut(s) 462, 654
SwaI ATTTAAAT 1 cut(s) 834
TaiI ACGT 1 cut(s) 522
TaqI TCGA 4 cut(s) 187, 480, 512, 728
TasI AATT 7 cut(s) 297, 590, 715, 733, 835, 853, 944
TatI WGTACW 1 cut(s) 68
TauI GCSGC 2 cut(s) 662, 975
TfiI GAWTC 2 cut(s) 952, 992
Tru1I TTAA 6 cut(s) 144, 191, 242, 276, 833, 839
Tru9I TTAA 6 cut(s) 144, 191, 242, 276, 833, 839
TseFI GTSAC 1 cut(s) 857
TseI GCWGC 6 cut(s) 363, 366, 369, 372, 541, 816
Tsp45I GTSAC 1 cut(s) 857
TspDTI ATGAA 4 cut(s) 128, 746, 761, 789
TspGWI ACGGA 2 cut(s) 109, 355
XapI RAATTY 4 cut(s) 590, 733, 835, 944
XceI RCATGY 1 cut(s) 461
XhoI CTCGAG 1 cut(s) 511
XmnI GAANNNNTTC 1 cut(s) 29
XspI CTAG 2 cut(s) 396, 420
ZrmI AGTACT 1 cut(s) 70
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.