MD11G1065500.v1.1

Rab3 GTPase-activating protein catalytic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0004790
Physical Location & Seq
Reverse (-)
5637302 .. 5654413
17112 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1065500.v1.1.491

Sequence Viewer

Length: 2925 bp
ATGGCATCATCCAGCAGGTTGGAATCTGTCGGAGACGATGAAGCTGAAGAGGAGGAGGTTGAGCATTTTGATGATTTCACTCTCGCCTCTTCATGGGAAAGGTTTATTTCCGATATTGAAGCGGTTTGTAGGCAATGGTTGGCAGACGGTCCGAAAAATCTGCTGAAAAAGGATGCAAGGGAGGTTTCGGAGGATTTATACAAGGTCAAGTCCGAGTTGAAGTTTTCAGCAAAAACTTATTGCATGGAATACTATTTTGGAACAAAATATGACGGCAAAGTTATCGATTGGAATTCCACATTGCATGACTTGCAGTTGTGTTTTGGGGTCAAAGAATTTTTGGTAATTGCTCCTCAAAGTGCCAGCGGTGTGGTTCTTGATGCACCTGAGGCTAGCAAGCTTTTAAGTGCAGTTGCAATTGCCTTATCAAATTGTTCAAGTTTGTGGCCTGCATTTGTTCCAGTACATGTTCCTGCTAGGAAAGCTTACATCGGTATCCAAAATATGGGAACAGTTTTTACTAGAAGATTTGAGGCTGATCATATTGGTAGCCAGGTTCCAGTAAAACTCATGCATTTGGAAGGATTGTATGAGTTGTTTGTTTCCAAATTTGCCTACTCCACATTGGACTTTTCTACTCACAGTTTCAAAGTCCATTTCACAATGAAGCTAACCTATAGATCCCTTCCCCATGATGATGAGGTTCAAGGAGATGATCCAGATGTTACTGAATCTGAAATAGATATTGGTGGTGAAATTCACAATAGAACACAATGGGATGATGATTGTCCATGGAGTGAGTGGTATTCTGCCGAAGACCCTGTAAAAGGATTTGAATTGGTTGCCATGTGGTCAGAGAAATTGCTTGAGAGCTCTATGGAAATGGCTGAGCTGGAAAATTCTTCACCCCATGAAGCTCAAAAATGGATACTATCTCCAAATATGTCTTCAGCTTTAACTGATTCTTCCAAAGGAAACAGAATTGGATTTTCTTCTCAACTGTGCCTATTACTTGACGCATTGAATATGTCCTTTGAGGCTCAATTTATGGAAGATTTTGTGTCAGTTGAAAATCCAGGTTCAGATAATTTGAAGTCCTCAATGGTTATACCTCCACCTACAGTTATTGATCGCGTGCTTAAGGAACTCTTTCATGATGGTGCACGATTCCTAGATGTTGCTGCTGCGGAGCATAAGACTTCTCGAGCTATTAAGGGCGCACCTCTTAAATCCCTTTTTGCACAGTTTTGTTTGCATTCTCTTTGGTTTGGAAACTGCAACATACGTGCTATTGCTGTTATCTGGATAGAGTTTGTTCGTGAAGTCCGGTGGTGTTGGGAAGAGTCACAGCCACTGCCCCTAATGCCAGCCACTGGTTCAATTGACCTCTCCACGTGTTTAATTAATCAGAAACTTCACATGCTTGCAATATGCATTGAGATGAAGCGTCAATTGAATGAAGACTTTCAAGATTGTGTTGGAAGCCAGGAGAATTCATCTCCTCAGACTGAGGAAGATAGTCAAGATGAGGATTCCTGTATAATGCAAACACCTGGTGAAAACTTTGATGGGAAACATGACAGCCCTGTAACACCAGATAATTTACAACACTCTGAAAAAAGTTTATCGAGTGGTAGTACTAAGCCAGAAGCTGTGGTATCTGCTAATCTGAAATCTTCAGATTCTACCAGGAGGGGTTCAGCTGGTGTGGCAGGATCTATGAAGCAGGAAGATGTGGTATCTGCTAATCTGAAATCTTCAGATTCTATCAGGAGGGGTTCAGCTGGTGTGGCAGGATCTATGATGCTTCTGAAGTCGTATCAGAGCATGCATGCGCCATACACTCAGGAGTCACCACTTATGACAGAAGACATGCACGAAGAACGGCTTCATGCCGTTGAAGCCTTGGGCGACTCATTTGACTTTTCTGCTCAGTTGGAGAGAGAGATCTTAACTTCAGACATGTCAGCGTTTAAAGCTGCAAATCCAGATGCTGTTTTTGAAGATTTTATTCGCTGGCATTCTCCCGGAGATTGGGAGAGCAATGACACAAAAGATACTGGATCATCCAACACTCCGGCAATAGAGGGTTCAAAGAATGACTGGCCTCCTCAAGGACGACTTTCAAAGAGAATGTCTGAGCATGGAAATTTATGGCGAAAGATTTGGAATGATGCACCTGCTTTGCCAGCTTATGAGCAGAAGCCGCTTCTGGATCCAAATAGAGAAGGAGAAAAGATCCTTCATTACCTGGAAACGTTACGACCACATCAGTTGCTTGAGCAAATGATTTGTACTGCCTTCAGAGCATCAGCAGATACTTTGAACCAGACTAGCTATGGAGGCTTGAAGCAGATGGAAACCAAAATGGATCAACTTTACATTACTTTGGCGTCTGCATTGAGGCCTCTGCAAGCAAATCGTCTATCTGCTGGCAGTGAGACTATTGAAGACATAAGACGGCTCTGTGGTGTTTTTGAACATGTCGAGAAGCTACTCACCATTGCAGCTTCCCTTCATCGCAAGTTCTTGCAAGCCCCACGCCTCTCTGAAGCCATTTTCTCCGACTGCTGCAGCTTTTATTTTCCTAGAATGGCAACAAGCTCATCAGGAGACAATGCTCCAAAGGAGTTTGACAAGAAGCAACAAGTAAGGATGCACGAAAGACCCATCGTGTCAAATATGTTTACCCCTCCAACTGCTAATCAGTCGTGGAGGAAAGTTTTAAGCTTGGGTAATCTTCTCAACGGCCACGAACCAATCTTGAGGGAGATAATATTTTCCAAGCGGGATAGAGTGAGCGGAAATCACTACGCTGCCCACACATCCCAAATTACTCAAGAAGAAGTAGAAACATATAGAATGTATTCATGTGGAACCTCGAACGATCTTCGCGTAGCTCTTTCTGTCGTTTCGTGTGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rab3-GTPase_cat

Protein Analysis

975

Amino Acids

109.25

Weight (kDa)

5.05

Isoelectric Point (pI)

53.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rab3-GTPase_cat PF13890 588 - 752 4.1e-58 Rab3 GTPase-activating protein catalytic subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2188
Acc36I ACCTGC 2 cut(s) 6, 2188
AccB7I CCANNNNNTGG 2 cut(s) 505, 1373
AccBSI CCGCTC 1 cut(s) 2802
AccII CGCG 2 cut(s) 1134, 2895
AciI CCGC 6 cut(s) 122, 366, 1187, 2207, 2788, 2802
AclI AACGTT 1 cut(s) 2258
AclWI GGATC 9 cut(s) 675, 710, 1723, 1804, 2071, 2210, 2223, 2233, 2379
AcoI YGGCCR 1 cut(s) 2749
AcsI RAATTY 7 cut(s) 292, 335, 608, 756, 898, 1492, 2149
AcuI CTGAAG 8 cut(s) 66, 933, 1662, 1743, 1832, 1941, 2287, 2571
AcvI CACGTG 1 cut(s) 1395
AcyI GRCGYC 1 cut(s) 2393
AdeI CACNNNGTG 1 cut(s) 1556
AfaI GTAC 3 cut(s) 465, 1639, 2296
AfiI CCNNNNNNNGG 6 cut(s) 93, 505, 827, 1373, 2034, 2114
AflII CTTAAG 1 cut(s) 1139
AflIII ACRYGT 4 cut(s) 466, 1394, 1962, 2482
AjnI CCWGG 6 cut(s) 552, 1075, 1485, 1552, 1688, 2250
AjuI GAANNNNNNNTTGG 6 cut(s) 240, 272, 729, 761, 931, 963
Alw21I GWGCWC 2 cut(s) 875, 1165
Alw26I GTCTC 3 cut(s) 27, 2435, 2607
Alw44I GTGCAC 1 cut(s) 1161
AlwI GGATC 9 cut(s) 675, 710, 1723, 1804, 2071, 2210, 2223, 2233, 2379
AlwNI CAGNNNCTG 4 cut(s) 1354, 1373, 1652, 1994
Ama87I CYCGRG 1 cut(s) 1203
AoxI GGCC 4 cut(s) 446, 2105, 2405, 2749
ApaLI GTGCAC 1 cut(s) 1161
ApeKI GCWGC 7 cut(s) 1181, 1184, 1979, 2507, 2571, 2574, 2816
ApoI RAATTY 7 cut(s) 292, 335, 608, 756, 898, 1492, 2149
ArsI GACNNNNNNTTYG 2 cut(s) 2120, 2152
AseI ATTAAT 1 cut(s) 1404
Asp700I GAANNNNTTC 4 cut(s) 1149, 1464, 1887, 2866
AspLEI GCGC 2 cut(s) 1220, 1837
AspS9I GGNCC 1 cut(s) 149
AsuC2I CCSGG 1 cut(s) 2028
AsuHPI GGTGA 5 cut(s) 764, 897, 1568, 1845, 2491
AsuNHI GCTAGC 1 cut(s) 392
AvaI CYCGRG 1 cut(s) 1203
AvaII GGWCC 1 cut(s) 149
AxyI CCTNAGG 1 cut(s) 387
BaeGI GKGCMC 1 cut(s) 1165
BaeI ACNNNNGTAYC 2 cut(s) 478, 511
BamHI GGATCC 1 cut(s) 2215
BanII GRGCYC 1 cut(s) 875
BbrPI CACGTG 1 cut(s) 1395
BbsI GAAGAC 5 cut(s) 822, 939, 1467, 1875, 2457
Bbv12I GWGCWC 2 cut(s) 875, 1165
BbvI GCAGC 7 cut(s) 1168, 1171, 1966, 2519, 2558, 2586, 2803
BccI CCATC 4 cut(s) 1151, 1562, 2350, 2678
BceAI ACGGC 5 cut(s) 289, 1880, 1901, 2477, 2764
BcgI CGANNNNNNTGC 6 cut(s) 142, 176, 265, 299, 2402, 2436
BciT130I CCWGG 6 cut(s) 554, 1077, 1487, 1554, 1690, 2252
BciVI GTATCC 2 cut(s) 506, 921
BclI TGATCA 1 cut(s) 538
BcnI CCSGG 1 cut(s) 2028
BcoDI GTCTC 3 cut(s) 27, 2435, 2607
BfaI CTAG 7 cut(s) 393, 477, 522, 1172, 2334, 2589, 2923
BfmI CTRYAG 3 cut(s) 676, 1119, 2572
BfrI CTTAAG 1 cut(s) 1139
BfuAI ACCTGC 2 cut(s) 6, 2188
BfuI GTATCC 2 cut(s) 506, 921
BglII AGATCT 1 cut(s) 1947
BisI GCNGC 8 cut(s) 1182, 1185, 1980, 2207, 2508, 2572, 2575, 2817
BlpI GCTNAGC 1 cut(s) 888
BlsI GCNGC 8 cut(s) 1183, 1186, 1981, 2208, 2509, 2573, 2576, 2818
BmcAI AGTACT 1 cut(s) 1639
Bme1390I CCNGG 7 cut(s) 554, 1077, 1487, 1554, 1690, 2028, 2252
Bme18I GGWCC 1 cut(s) 149
BmeT110I CYCGRG 1 cut(s) 1203
BmgT120I GGNCC 1 cut(s) 149
BmiI GGNNCC 3 cut(s) 558, 2217, 2878
BmrFI CCNGG 7 cut(s) 554, 1077, 1487, 1554, 1690, 2028, 2252
BmsI GCATC 8 cut(s) 14, 163, 370, 1794, 1981, 2164, 2318, 2646
BmtI GCTAGC 1 cut(s) 396
BpiI GAAGAC 5 cut(s) 822, 939, 1467, 1875, 2457
Bpu1102I GCTNAGC 1 cut(s) 888
BpuEI CTTGAG 5 cut(s) 887, 2097, 2300, 2785, 2823
BpuMI CCSGG 1 cut(s) 2028
Bsa29I ATCGAT 1 cut(s) 285
BsaAI YACGTR 2 cut(s) 1286, 1395
BsaHI GRCGYC 1 cut(s) 2393
BsaJI CCNNGG 2 cut(s) 791, 1905
BsaWI WCCGGW 1 cut(s) 1326
Bsc4I CCNNNNNNNGG 6 cut(s) 93, 505, 827, 1373, 2034, 2114
Bse1I ACTGG 5 cut(s) 461, 560, 1378, 2065, 2108
Bse21I CCTNAGG 1 cut(s) 387
Bse3DI GCAATG 4 cut(s) 140, 299, 2050, 2502
BseBI CCWGG 6 cut(s) 554, 1077, 1487, 1554, 1690, 2252
BseCI ATCGAT 1 cut(s) 285
BseDI CCNNGG 2 cut(s) 791, 1905
BseGI GGATG 6 cut(s) 8, 178, 784, 2066, 2661, 2825
BseLI CCNNNNNNNGG 6 cut(s) 93, 505, 827, 1373, 2034, 2114
BseMI GCAATG 4 cut(s) 140, 299, 2050, 2502
BseMII CTCAG 7 cut(s) 378, 879, 1500, 1517, 1859, 1946, 2130
BseNI ACTGG 5 cut(s) 461, 560, 1378, 2065, 2108
BseRI GAGGAG 5 cut(s) 65, 68, 342, 1491, 2100
BseSI GKGCMC 1 cut(s) 1165
BseXI GCAGC 7 cut(s) 1168, 1171, 1966, 2519, 2558, 2586, 2803
BsgI GTGCAG 1 cut(s) 429
Bsh1236I CGCG 2 cut(s) 1134, 2895
BshFI GGCC 4 cut(s) 448, 2107, 2407, 2751
BshVI ATCGAT 1 cut(s) 285
BsiHKAI GWGCWC 2 cut(s) 875, 1165
BsiHKCI CYCGRG 1 cut(s) 1203
BsiSI CCGG 3 cut(s) 1327, 2028, 2078
BslI CCNNNNNNNGG 6 cut(s) 93, 505, 827, 1373, 2034, 2114
BsmAI GTCTC 3 cut(s) 27, 2435, 2607
BsmBI CGTCTC 1 cut(s) 27
BsmI GAATGC 2 cut(s) 1255, 2020
BsnI GGCC 4 cut(s) 448, 2107, 2407, 2751
BsoBI CYCGRG 1 cut(s) 1203
Bsp1286I GDGCHC 2 cut(s) 875, 1165
Bsp1720I GCTNAGC 1 cut(s) 888
Bsp19I CCATGG 1 cut(s) 791
BspACI CCGC 6 cut(s) 122, 366, 1187, 2207, 2788, 2802
BspANI GGCC 4 cut(s) 448, 2107, 2407, 2751
BspCNI CTCAG 7 cut(s) 379, 880, 1501, 1516, 1858, 1945, 2131
BspDI ATCGAT 1 cut(s) 285
BspFNI CGCG 2 cut(s) 1134, 2895
BspHI TCATGA 1 cut(s) 1153
BspLI GGNNCC 3 cut(s) 558, 2217, 2878
BspMAI CTGCAG 1 cut(s) 2576
BspMI ACCTGC 2 cut(s) 6, 2188
BspOI GCTAGC 1 cut(s) 396
BspPI GGATC 9 cut(s) 675, 710, 1723, 1804, 2071, 2210, 2223, 2233, 2379
BspTI CTTAAG 1 cut(s) 1139
BsrBI CCGCTC 1 cut(s) 2802
BsrDI GCAATG 4 cut(s) 140, 299, 2050, 2502
BsrI ACTGG 5 cut(s) 461, 560, 1378, 2065, 2108
BssECI CCNNGG 2 cut(s) 791, 1905
BssNI GRCGYC 1 cut(s) 2393
BssT1I CCWWGG 2 cut(s) 791, 1905
Bst2UI CCWGG 6 cut(s) 554, 1077, 1487, 1554, 1690, 2252
Bst4CI ACNGT 6 cut(s) 149, 514, 644, 1002, 1123, 1245
Bst6I CTCTTC 3 cut(s) 42, 94, 1335
BstACI GRCGYC 1 cut(s) 2393
BstAFI CTTAAG 1 cut(s) 1139
BstAPI GCANNNNNTGC 1 cut(s) 310
BstBAI YACGTR 2 cut(s) 1286, 1395
BstDEI CTNAG 8 cut(s) 387, 888, 1503, 1509, 1641, 1845, 1932, 2139
BstDSI CCRYGG 1 cut(s) 791
BstENI CCTNNNNNAGG 2 cut(s) 825, 2112
BstF5I GGATG 6 cut(s) 8, 178, 784, 2066, 2661, 2825
BstFNI CGCG 2 cut(s) 1134, 2895
BstHHI GCGC 2 cut(s) 1220, 1837
BstMAI GTCTC 3 cut(s) 27, 2435, 2607
BstNI CCWGG 6 cut(s) 554, 1077, 1487, 1554, 1690, 2252
BstNSI RCATGY 7 cut(s) 470, 1423, 1831, 1835, 1876, 1966, 2486
BstSCI CCNGG 7 cut(s) 552, 1075, 1485, 1552, 1688, 2026, 2250
BstSFI CTRYAG 3 cut(s) 676, 1119, 2572
BstSLI GKGCMC 1 cut(s) 1165
BstUI CGCG 2 cut(s) 1134, 2895
BstV1I GCAGC 7 cut(s) 1168, 1171, 1966, 2519, 2558, 2586, 2803
BstV2I GAAGAC 5 cut(s) 822, 939, 1467, 1875, 2457
BstX2I RGATCY 6 cut(s) 680, 1715, 1796, 1947, 2215, 2238
BstXI CCANNNNNNTGG 2 cut(s) 19, 370
BstYI RGATCY 6 cut(s) 680, 1715, 1796, 1947, 2215, 2238
Bsu15I ATCGAT 1 cut(s) 285
Bsu36I CCTNAGG 1 cut(s) 387
BsuI GTATCC 2 cut(s) 506, 921
BsuRI GGCC 4 cut(s) 448, 2107, 2407, 2751
BsuTUI ATCGAT 1 cut(s) 285
BtgI CCRYGG 1 cut(s) 791
BtgZI GCGATG 1 cut(s) 2504
BtsCI GGATG 6 cut(s) 8, 178, 784, 2066, 2661, 2825
BtsI GCAGTG 2 cut(s) 1352, 2443
BtsIMutI CAGTG 3 cut(s) 1352, 1371, 2443
BveI ACCTGC 2 cut(s) 6, 2188
CaiI CAGNNNCTG 4 cut(s) 1354, 1373, 1652, 1994
CciI TCATGA 1 cut(s) 1153
CfoI GCGC 2 cut(s) 1220, 1837
Cfr13I GGNCC 1 cut(s) 149
ClaI ATCGAT 1 cut(s) 285
CpoI CGGWCCG 1 cut(s) 149
CseI GACGC 3 cut(s) 1025, 1436, 2382
CsiI ACCWGGT 1 cut(s) 1552
Csp6I GTAC 3 cut(s) 464, 1638, 2295
CspCI CAANNNNNGTGG 2 cut(s) 2256, 2291
CspI CGGWCCG 1 cut(s) 149
CviQI GTAC 3 cut(s) 464, 1638, 2295
DdeI CTNAG 8 cut(s) 387, 888, 1503, 1509, 1641, 1845, 1932, 2139
DraI TTTAAA 1 cut(s) 1975
DraIII CACNNNGTG 1 cut(s) 1556
EaeI YGGCCR 1 cut(s) 2749
Eam1104I CTCTTC 3 cut(s) 42, 94, 1335
EarI CTCTTC 3 cut(s) 42, 94, 1335
Ecl136II GAGCTC 1 cut(s) 873
Eco130I CCWWGG 2 cut(s) 791, 1905
Eco147I AGGCCT 1 cut(s) 2407
Eco24I GRGCYC 1 cut(s) 875
Eco47I GGWCC 1 cut(s) 149
Eco53kI GAGCTC 1 cut(s) 873
Eco57I CTGAAG 8 cut(s) 66, 933, 1662, 1743, 1832, 1941, 2287, 2571
Eco72I CACGTG 1 cut(s) 1395
Eco81I CCTNAGG 1 cut(s) 387
Eco88I CYCGRG 1 cut(s) 1203
EcoICRI GAGCTC 1 cut(s) 873
EcoNI CCTNNNNNAGG 2 cut(s) 825, 2112
EcoRI GAATTC 2 cut(s) 292, 1492
EcoRII CCWGG 6 cut(s) 552, 1075, 1485, 1552, 1688, 2250
EcoT14I CCWWGG 2 cut(s) 791, 1905
EcoT22I ATGCAT 3 cut(s) 576, 1436, 1833
EcoT38I GRGCYC 1 cut(s) 875
ErhI CCWWGG 2 cut(s) 791, 1905
Esp3I CGTCTC 1 cut(s) 27
FalI AAGNNNNNCTT 6 cut(s) 1133, 1165, 1872, 1904, 2106, 2138
FauI CCCGC 1 cut(s) 2781
FbaI TGATCA 1 cut(s) 538
Fnu4HI GCNGC 8 cut(s) 1182, 1185, 1980, 2207, 2508, 2572, 2575, 2817
FokI GGATG 5 cut(s) 185, 791, 2053, 2668, 2812
FriOI GRGCYC 1 cut(s) 875
Fsp4HI GCNGC 8 cut(s) 1182, 1185, 1980, 2207, 2508, 2572, 2575, 2817
FspBI CTAG 7 cut(s) 393, 477, 522, 1172, 2334, 2589, 2923
GlaI GCGC 2 cut(s) 1219, 1836
GluI GCNGC 8 cut(s) 1182, 1185, 1980, 2207, 2508, 2572, 2575, 2817
HaeIII GGCC 4 cut(s) 448, 2107, 2407, 2751
HapII CCGG 3 cut(s) 1327, 2028, 2078
HgaI GACGC 3 cut(s) 1025, 1436, 2382
HhaI GCGC 2 cut(s) 1220, 1837
Hin1I GRCGYC 1 cut(s) 2393
Hin6I GCGC 2 cut(s) 1218, 1835
HinP1I GCGC 2 cut(s) 1218, 1835
HindIII AAGCTT 3 cut(s) 398, 483, 2728
HpaII CCGG 3 cut(s) 1327, 2028, 2078
HphI GGTGA 5 cut(s) 764, 897, 1568, 1845, 2491
Hpy166II GTNNAC 2 cut(s) 1163, 2688
Hpy8I GTNNAC 2 cut(s) 1163, 2688
HpyAV CCTTC 6 cut(s) 575, 695, 2222, 2252, 2311, 2525
HpyCH4III ACNGT 6 cut(s) 149, 514, 644, 1002, 1123, 1245
HpyCH4IV ACGT 3 cut(s) 1285, 1394, 2258
HpyF3I CTNAG 8 cut(s) 387, 888, 1503, 1509, 1641, 1845, 1932, 2139
HpySE526I ACGT 3 cut(s) 1285, 1394, 2258
Hsp92I GRCGYC 1 cut(s) 2393
HspAI GCGC 2 cut(s) 1218, 1835
Ksp22I TGATCA 1 cut(s) 538
LmnI GCTCC 3 cut(s) 355, 1189, 2626
Lsp1109I GCAGC 7 cut(s) 1168, 1171, 1966, 2519, 2558, 2586, 2803
LweI GCATC 8 cut(s) 14, 163, 370, 1794, 1981, 2164, 2318, 2646
MabI ACCWGGT 1 cut(s) 1552
MaeI CTAG 7 cut(s) 393, 477, 522, 1172, 2334, 2589, 2923
MaeII ACGT 3 cut(s) 1285, 1394, 2258
MaeIII GTNAC 6 cut(s) 724, 1344, 1588, 1851, 2259, 2918
MbiI CCGCTC 1 cut(s) 2802
MfeI CAATTG 3 cut(s) 417, 1380, 1451
MflI RGATCY 6 cut(s) 680, 1715, 1796, 1947, 2215, 2238
MhlI GDGCHC 2 cut(s) 875, 1165
MlyI GAGTC 3 cut(s) 1352, 1859, 1907
MmeI TCCRAC 6 cut(s) 10, 1459, 1917, 2094, 2589, 2720
Mph1103I ATGCAT 3 cut(s) 576, 1436, 1833
MroXI GAANNNNTTC 4 cut(s) 1149, 1464, 1887, 2866
MslI CAYNNNNRTG 4 cut(s) 69, 696, 1158, 1439
MspA1I CMGCKG 3 cut(s) 366, 1703, 1784
MspCI CTTAAG 1 cut(s) 1139
MspI CCGG 3 cut(s) 1327, 2028, 2078
MspR9I CCNGG 7 cut(s) 554, 1077, 1487, 1554, 1690, 2028, 2252
MunI CAATTG 3 cut(s) 417, 1380, 1451
Mva1269I GAATGC 2 cut(s) 1255, 2020
MvaI CCWGG 6 cut(s) 554, 1077, 1487, 1554, 1690, 2252
MvnI CGCG 2 cut(s) 1134, 2895
NciI CCSGG 1 cut(s) 2028
NcoI CCATGG 1 cut(s) 791
NheI GCTAGC 1 cut(s) 392
NlaIV GGNNCC 3 cut(s) 558, 2217, 2878
NmuCI GTSAC 3 cut(s) 1344, 1851, 2918
NsiI ATGCAT 3 cut(s) 576, 1436, 1833
NspI RCATGY 7 cut(s) 470, 1423, 1831, 1835, 1876, 1966, 2486
PacI TTAATTAA 1 cut(s) 1404
PaeI GCATGC 2 cut(s) 1831, 1835
PaeR7I CTCGAG 1 cut(s) 1203
PagI TCATGA 1 cut(s) 1153
PaqCI CACCTGC 1 cut(s) 2188
PceI AGGCCT 1 cut(s) 2407
PciI ACATGT 3 cut(s) 466, 1962, 2482
PcsI WCGNNNNNNNCGW 1 cut(s) 2892
PctI GAATGC 2 cut(s) 1255, 2020
PdmI GAANNNNTTC 4 cut(s) 1149, 1464, 1887, 2866
PfeI GAWTC 7 cut(s) 23, 731, 962, 1167, 1532, 1682, 1763
PflMI CCANNNNNTGG 2 cut(s) 505, 1373
PfoI TCCNGGA 1 cut(s) 2026
PkrI GCNGC 8 cut(s) 1183, 1186, 1981, 2208, 2509, 2573, 2576, 2818
PleI GAGTC 3 cut(s) 1351, 1858, 1907
PmaCI CACGTG 1 cut(s) 1395
PmlI CACGTG 1 cut(s) 1395
PpsI GAGTC 3 cut(s) 1351, 1858, 1907
Ppu21I YACGTR 2 cut(s) 1286, 1395
PscI ACATGT 3 cut(s) 466, 1962, 2482
PshBI ATTAAT 1 cut(s) 1404
Psp124BI GAGCTC 1 cut(s) 875
Psp1406I AACGTT 1 cut(s) 2258
Psp6I CCWGG 6 cut(s) 552, 1075, 1485, 1552, 1688, 2250
PspCI CACGTG 1 cut(s) 1395
PspGI CCWGG 6 cut(s) 552, 1075, 1485, 1552, 1688, 2250
PspN4I GGNNCC 3 cut(s) 558, 2217, 2878
PspPI GGNCC 1 cut(s) 149
PsrI GAACNNNNNNTAC 2 cut(s) 502, 534
PstI CTGCAG 1 cut(s) 2576
PstNI CAGNNNCTG 4 cut(s) 1354, 1373, 1652, 1994
PsuI RGATCY 6 cut(s) 680, 1715, 1796, 1947, 2215, 2238
PvuII CAGCTG 2 cut(s) 1703, 1784
RsaI GTAC 3 cut(s) 465, 1639, 2296
RsaNI GTAC 3 cut(s) 464, 1638, 2295
RseI CAYNNNNRTG 4 cut(s) 69, 696, 1158, 1439
Rsr2I CGGWCCG 1 cut(s) 149
RsrII CGGWCCG 1 cut(s) 149
SacI GAGCTC 1 cut(s) 875
SatI GCNGC 8 cut(s) 1182, 1185, 1980, 2207, 2508, 2572, 2575, 2817
Sau96I GGNCC 1 cut(s) 149
ScaI AGTACT 1 cut(s) 1639
SchI GAGTC 3 cut(s) 1352, 1859, 1907
ScrFI CCNGG 7 cut(s) 554, 1077, 1487, 1554, 1690, 2028, 2252
SduI GDGCHC 2 cut(s) 875, 1165
SexAI ACCWGGT 1 cut(s) 1552
SfaNI GCATC 8 cut(s) 14, 163, 370, 1794, 1981, 2164, 2318, 2646
SfcI CTRYAG 3 cut(s) 676, 1119, 2572
Sfr274I CTCGAG 1 cut(s) 1203
SinI GGWCC 1 cut(s) 149
SlaI CTCGAG 1 cut(s) 1203
SmiMI CAYNNNNRTG 4 cut(s) 69, 696, 1158, 1439
SmlI CTYRAG 7 cut(s) 866, 1139, 1203, 2112, 2279, 2764, 2838
SmoI CTYRAG 7 cut(s) 866, 1139, 1203, 2112, 2279, 2764, 2838
SphI GCATGC 2 cut(s) 1831, 1835
SseBI AGGCCT 1 cut(s) 2407
SsiI CCGC 6 cut(s) 122, 366, 1187, 2207, 2788, 2802
SspI AATATT 1 cut(s) 2778
SspMI CTAG 7 cut(s) 393, 477, 522, 1172, 2334, 2589, 2923
SstI GAGCTC 1 cut(s) 875
StuI AGGCCT 1 cut(s) 2407
StyD4I CCNGG 7 cut(s) 552, 1075, 1485, 1552, 1688, 2026, 2250
StyI CCWWGG 2 cut(s) 791, 1905
TaaI ACNGT 6 cut(s) 149, 514, 644, 1002, 1123, 1245
TaiI ACGT 3 cut(s) 1288, 1397, 2261
TaqI TCGA 5 cut(s) 285, 1204, 1628, 2487, 2882
TatI WGTACW 3 cut(s) 463, 1637, 2294
TauI GCSGC 1 cut(s) 2209
TfiI GAWTC 7 cut(s) 23, 731, 962, 1167, 1532, 1682, 1763
TscAI CASTG 3 cut(s) 1359, 1378, 2443
TseFI GTSAC 3 cut(s) 1344, 1851, 2918
TseI GCWGC 7 cut(s) 1181, 1184, 1979, 2507, 2571, 2574, 2816
Tsp45I GTSAC 3 cut(s) 1344, 1851, 2918
TspRI CASTG 3 cut(s) 1359, 1378, 2443
Van91I CCANNNNNTGG 2 cut(s) 505, 1373
Vha464I CTTAAG 1 cut(s) 1139
VneI GTGCAC 1 cut(s) 1161
VpaK11BI GGWCC 1 cut(s) 149
VspI ATTAAT 1 cut(s) 1404
XagI CCTNNNNNAGG 2 cut(s) 825, 2112
XapI RAATTY 7 cut(s) 292, 335, 608, 756, 898, 1492, 2149
XceI RCATGY 7 cut(s) 470, 1423, 1831, 1835, 1876, 1966, 2486
XcmI CCANNNNNNNNNTGG 2 cut(s) 798, 2336
XhoI CTCGAG 1 cut(s) 1203
XmnI GAANNNNTTC 4 cut(s) 1149, 1464, 1887, 2866
XspI CTAG 7 cut(s) 393, 477, 522, 1172, 2334, 2589, 2923
ZrmI AGTACT 1 cut(s) 1639
Zsp2I ATGCAT 3 cut(s) 576, 1436, 1833
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.