Rh5DG495100

Rab3 GTPase-activating protein catalytic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0004790
Physical Location & Seq
Forward (+)
77620333 .. 77628749
8417 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG495100.1

Sequence Viewer

Length: 2865 bp
ATGGCTTCTTCCAGCAAGATGGAATCTTCCGCAGACGACCAAGCTCAACAGGAGGAGGAGGACGACGAGGTTGAGCATTTTGATGATTTCACTCTTGCCTCTTCATGGGAAAGGTTCATTTCTGATATAGAGGCGGTTTGTAGGCAATGGATAGCCGACGGTCCGAAAAATCTGTTGAAAAAGGATGCAAGGGAGGTTTCAGAGGAGTTTTATAAGGTTAAATATGAGTTGAAGTTTGCAGTGAAAAGTTACTGCATGGAATACTACTTTGGATCTAAAAATGACGGTAAAGTTATGGATTGGGATCCCAGATTGCATGATTTGCAGTTGTGCTTTGGGGTCAAAGATTTTTTGGTAATTGCTCCACAAAGTGCCAGCGGAGTGGTTCTTGACGCGCCTGAGGCTAGCAAGCTTTTAAGTGCAGTTGCAATTGCTTTATCAAATTGTTCAAGTTTGTGGCCCGCATTTGTCCCGGTGCATGTTCCTGCTAGGAAAGCTTACATTGGCATTCAGAATATGGGAACAGTTTTCACTAGAAGATTTGAGGCTGATCATATTGGTAGCCAGGTTCCAGTAAAACTTATGCATTTGGAAGGATTATACGAGTTGTTTGTTTCTAAATTTGCCTACTCCACATTGGACTTTTCCATGCATCTCTTCAAAGTCCATTTTACTATGAAGCTAACCTACAGATCCCTTCCGCCTGATGACGATGATGTACAAAGAGCTGATGCTGATGTTACTGAATCTGGAGACGATCTTGGTGGTGACATGCACAATAGAACACAATGGGATGATGATTGTCCATGGAGTGAGTGGTATTCTGCTGAAGACCCTGTAAAAGGATTTGAGTTGGTTGCCTTGTGGTCTGAAAAGGTGGTCGAGAGCTCCCTGGAGATGGCTGAACTGGAAAATTCTTCACCCCATGAGGCTCAAAAATGGATATTATCTCCTAATATGTCTTCAACTTTAACTGATTCTTCCAAAGGAAACAGAATTGGATTTGCTAGCCAGCTGTGCCTTTTGGTTGATGCATTGAACAAGTCGTTTGAGGCTCAGTTTATGGAAGATTTCGTGTCAGTTGAAAACCCAGGTTCTGATAATTTGAACTCCTCCATGGTTATACCTCCTCCTACAGTTATTGATCGTGTGCTCAAGGAACTCTTTCATGATGGTGCACAATTCCCCAGTTTTGCCTCTGGTGAGCACAAGACTTCTCGAATAAAGGCCGCACCTCTGGAATCCCTTTTTGCACAGTTTTGCCTGCATTCTCTTTGGTTTGGAAACTGCAATATACGTGCTATTTCTGTTATCTGGATAGAGTTTGTTCGAGAAGTTCGGTGGTGTTGGGAAGAGTCACAGCCATTGCCCTTAATGTCAGCCTTTGGTTCAATTGATCTGTCCACTTGTGTGATTTACCAGAAACTTCATATGCTTGCTATATGCATCGAGAGGAAGCGTCAATTGAATGAAGAATTTCAAGATTGTGTTGGAAGCATGGATCATTCATCTCCTAGTATTGAGGAAGATGGTCTGGATGAGGGGTCAGAGGACTCATGTATTATGCAAACACCTGGTGAAAACCTTGATGGGAAACGAGACAGCCCTGCAATTCCAGATGATTTGCAAGACTCTAAACAGAGTGTATCAAGTCTTGGTGCTAGGCCTGAACATGCTGCGGTGTCTGCTGATCTAAAACCCTCAGATTGTATCAGAAGGGGTTCAGCTGGTGAGGCAGGTATGATGCTTCTGAAATCAAAACAGAGAATGCATGCACCATTCACGCAGGAGGCACCACTCATGACAGAAGACATGCATGAAGAACGGCTCCAGGCCGTGGAAGCCTTTGGTGACTCACTTAACTTTTCTGCTTTTTTGGAGAGAGAGATCTTAGCTTCAGACATGTCAGCATTCAAAGCAGCAAATCCAGACGCTGTTTTTGAAGATTTTATTCGCTGGCATTCTCCTGGAGATTGGGAGAGTGATGTTACAAAAGAGAGCGGATCATCCAATAATCCTGCAACAGAGGGTTCAAAAGATGACTGGCCCCCTCGAGGGCGGCTGTCAAAGAGAATGTCTGAGAGTGGGAATTTGTGGCGAAAGATTTGGAATGACGCACCTGCTTTGCCAGCTTCTGAGCAGAAGCCGCTAATGGATCCAAATAGAGAGGGAGAAAAGAGTCTTCATTACCTGGAAACCTTACGACCACATCAGTTGCTCGAGCAAATGATATGTACTGCCTTCAGCGCGGCAGCAGATACTTTGAATCAGACTCATTTTGGAGGCTTGAAGCAGATGACAACCAAACTCGATCAACTTTACCTTACTATGACATCTGCATTGAGGCCTTTGCAAGCAAATGGGCTATCTGCTGACACTGATACTATTGAAGACATAAAACGGCTGTGTGGTGTTTTTGAACATGTTGAGAAGCTACTCACTATTGCAGCTTCTCTTCATCGCAAGTTCTTGCACGCCCCACGCCTCTCTGAAGCAATATTCCGTGACTGCTGCAACTTTTATTTTCCAAGGATGGGAACAAGCTCCTCAGGCGACAATACTCCAAAGGAGTTTGAGAAGCAGCAAATAGTAACGATGAACGAAAGACCGGTGGTGTCAAATATGTTTACGCCTCCCACTGCTAACCAGTCATGGCGAAAAGTTTTGAGCTTGGGTAATCTTCTCAACGGCCATGAACCCGTCTTGAGAGAGATCATCTTTTCCAAGCGTGATAAAGTGAGCGGAAATCACTATGCTGCCCGTACTCCAAATGCTTATCAAGATGAACTAGAAACATATAGAATGTACATATGTGGAACCTCAAATGACCTCCGTATAGCACTTTCTGTTGTTTCTTGTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rab3-GTPase_cat

Protein Analysis

954

Amino Acids

107.24

Weight (kDa)

4.91

Isoelectric Point (pI)

51.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rab3-GTPase_cat PF13890 572 - 732 2.9e-54 Rab3 GTPase-activating protein catalytic subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 213
AarI CACCTGC 1 cut(s) 2128
AbsI CCTCGAGG 1 cut(s) 2052
Acc36I ACCTGC 2 cut(s) 1727, 2128
AccB1I GGYRCC 1 cut(s) 1792
AccB7I CCANNNNNTGG 1 cut(s) 1837
AccBSI CCGCTC 2 cut(s) 2001, 2742
AccII CGCG 2 cut(s) 395, 2249
AclWI GGATC 8 cut(s) 280, 299, 312, 687, 1509, 2011, 2150, 2163
AcoI YGGCCR 1 cut(s) 2689
AcsI RAATTY 4 cut(s) 620, 913, 1475, 2089
AcuI CTGAAG 4 cut(s) 849, 1881, 2227, 2511
AdeI CACNNNGTG 2 cut(s) 371, 1577
AfaI GTAC 4 cut(s) 720, 2236, 2764, 2807
AfiI CCNNNNNNNGG 7 cut(s) 105, 842, 898, 1837, 2054, 2055, 2534
AflIII ACRYGT 2 cut(s) 1902, 2422
AgeI ACCGGT 1 cut(s) 2608
AjnI CCWGG 7 cut(s) 564, 891, 1090, 1573, 1830, 1966, 2190
AjuI GAANNNNNNNTTGG 2 cut(s) 252, 284
AleI CACNNNNGTG 1 cut(s) 1409
Alw21I GWGCWC 4 cut(s) 890, 1155, 1180, 1209
Alw26I GTCTC 2 cut(s) 747, 1593
Alw44I GTGCAC 1 cut(s) 1176
AlwI GGATC 8 cut(s) 280, 299, 312, 687, 1509, 2011, 2150, 2163
AlwNI CAGNNNCTG 3 cut(s) 1097, 1934, 2135
Ama87I CYCGRG 2 cut(s) 2052, 2219
AoxI GGCC 7 cut(s) 458, 1227, 1664, 1833, 2045, 2345, 2689
ApaLI GTGCAC 1 cut(s) 1176
ApeKI GCWGC 7 cut(s) 1676, 1919, 2252, 2447, 2511, 2581, 2756
ApoI RAATTY 4 cut(s) 620, 913, 1475, 2089
ArsI GACNNNNNNTTYG 4 cut(s) 1922, 1954, 2060, 2092
AsiGI ACCGGT 1 cut(s) 2608
Asp700I GAANNNNTTC 3 cut(s) 1164, 1476, 1720
AspLEI GCGC 2 cut(s) 397, 2249
AspS9I GGNCC 3 cut(s) 161, 459, 2046
AsuC2I CCSGG 1 cut(s) 473
AsuHPI GGTGA 6 cut(s) 779, 912, 1214, 1589, 1742, 1862
AsuNHI GCTAGC 2 cut(s) 404, 1007
AvaI CYCGRG 2 cut(s) 2052, 2219
AvaII GGWCC 1 cut(s) 161
AxyI CCTNAGG 2 cut(s) 399, 2548
BaeGI GKGCMC 1 cut(s) 1180
BamHI GGATCC 2 cut(s) 304, 2155
BanI GGYRCC 1 cut(s) 1792
BanII GRGCYC 1 cut(s) 890
BarI GAAGNNNNNNTAC 2 cut(s) 671, 703
BbsI GAAGAC 5 cut(s) 837, 954, 1815, 2174, 2397
Bbv12I GWGCWC 4 cut(s) 890, 1155, 1180, 1209
BbvI GCAGC 7 cut(s) 1663, 1931, 2264, 2459, 2498, 2593, 2743
BccI CCATC 6 cut(s) 13, 892, 1166, 1523, 1583, 2527
BceAI ACGGC 4 cut(s) 1820, 1841, 2417, 2704
BciT130I CCWGG 7 cut(s) 566, 893, 1092, 1575, 1832, 1968, 2192
BclI TGATCA 1 cut(s) 550
BcnI CCSGG 1 cut(s) 473
BcoDI GTCTC 2 cut(s) 747, 1593
BfaI CTAG 7 cut(s) 405, 489, 534, 1008, 1515, 1662, 2789
BfmI CTRYAG 2 cut(s) 688, 1134
BfuAI ACCTGC 2 cut(s) 1727, 2128
BglII AGATCT 1 cut(s) 1887
Bme1390I CCNGG 8 cut(s) 473, 566, 893, 1092, 1575, 1832, 1968, 2192
Bme18I GGWCC 1 cut(s) 161
BmeT110I CYCGRG 2 cut(s) 2052, 2219
BmgT120I GGNCC 3 cut(s) 161, 459, 2046
BmiI GGNNCC 7 cut(s) 306, 570, 1794, 1829, 2048, 2157, 2818
BmrFI CCNGG 8 cut(s) 473, 566, 893, 1092, 1575, 1832, 1968, 2192
BmrI ACTGGG 1 cut(s) 1182
BmsI GCATC 6 cut(s) 175, 661, 721, 1021, 1455, 1734
BmtI GCTAGC 2 cut(s) 408, 1011
BmuI ACTGGG 1 cut(s) 1182
BpiI GAAGAC 5 cut(s) 837, 954, 1815, 2174, 2397
BpmI CTGGAG 4 cut(s) 771, 914, 1814, 1989
BpuEI CTTGAG 2 cut(s) 1139, 2725
BpuMI CCSGG 1 cut(s) 473
BsaAI YACGTR 1 cut(s) 1298
BsaBI GATNNNNATC 1 cut(s) 303
BsaJI CCNNGG 6 cut(s) 806, 891, 1090, 1116, 1836, 2528
BsaWI WCCGGW 1 cut(s) 2608
Bsc4I CCNNNNNNNGG 7 cut(s) 105, 842, 898, 1837, 2054, 2055, 2534
Bse118I RCCGGY 1 cut(s) 2608
Bse1I ACTGG 5 cut(s) 572, 912, 1188, 2048, 2647
Bse21I CCTNAGG 2 cut(s) 399, 2548
Bse3DI GCAATG 2 cut(s) 152, 1364
Bse8I GATNNNNATC 1 cut(s) 303
BseBI CCWGG 7 cut(s) 566, 893, 1092, 1575, 1832, 1968, 2192
BseDI CCNNGG 6 cut(s) 806, 891, 1090, 1116, 1836, 2528
BseGI GGATG 5 cut(s) 190, 799, 1543, 2006, 2538
BseJI GATNNNNATC 1 cut(s) 303
BseLI CCNNNNNNNGG 7 cut(s) 105, 842, 898, 1837, 2054, 2055, 2534
BseMI GCAATG 2 cut(s) 152, 1364
BseMII CTCAG 6 cut(s) 390, 1070, 1716, 2070, 2127, 2562
BseNI ACTGG 5 cut(s) 572, 912, 1188, 2048, 2647
BseRI GAGGAG 6 cut(s) 68, 71, 218, 1102, 1119, 2536
BseSI GKGCMC 1 cut(s) 1180
BseXI GCAGC 7 cut(s) 1663, 1931, 2264, 2459, 2498, 2593, 2743
BsgI GTGCAG 1 cut(s) 441
Bsh1236I CGCG 2 cut(s) 395, 2249
BshFI GGCC 7 cut(s) 460, 1229, 1666, 1835, 2047, 2347, 2691
BshNI GGYRCC 1 cut(s) 1792
BshTI ACCGGT 1 cut(s) 2608
BsiHKAI GWGCWC 4 cut(s) 890, 1155, 1180, 1209
BsiHKCI CYCGRG 2 cut(s) 2052, 2219
BsiSI CCGG 2 cut(s) 473, 2609
BslFI GGGAC 1 cut(s) 455
BslI CCNNNNNNNGG 7 cut(s) 105, 842, 898, 1837, 2054, 2055, 2534
BsmAI GTCTC 2 cut(s) 747, 1593
BsmBI CGTCTC 1 cut(s) 747
BsmFI GGGAC 1 cut(s) 455
BsmI GAATGC 5 cut(s) 507, 1267, 1773, 1910, 1960
BsnI GGCC 7 cut(s) 460, 1229, 1666, 1835, 2047, 2347, 2691
BsoBI CYCGRG 2 cut(s) 2052, 2219
Bsp1286I GDGCHC 4 cut(s) 890, 1155, 1180, 1209
Bsp1407I TGTACA 2 cut(s) 718, 2805
Bsp19I CCATGG 2 cut(s) 806, 1116
BspANI GGCC 7 cut(s) 460, 1229, 1666, 1835, 2047, 2347, 2691
BspCNI CTCAG 6 cut(s) 391, 1069, 1715, 2071, 2128, 2561
BspFNI CGCG 2 cut(s) 395, 2249
BspHI TCATGA 2 cut(s) 1168, 1800
BspLI GGNNCC 7 cut(s) 306, 570, 1794, 1829, 2048, 2157, 2818
BspMI ACCTGC 2 cut(s) 1727, 2128
BspOI GCTAGC 2 cut(s) 408, 1011
BspPI GGATC 8 cut(s) 280, 299, 312, 687, 1509, 2011, 2150, 2163
BspT107I GGYRCC 1 cut(s) 1792
BsrBI CCGCTC 2 cut(s) 2001, 2742
BsrDI GCAATG 2 cut(s) 152, 1364
BsrFI RCCGGY 1 cut(s) 2608
BsrGI TGTACA 2 cut(s) 718, 2805
BsrI ACTGG 5 cut(s) 572, 912, 1188, 2048, 2647
BssAI RCCGGY 1 cut(s) 2608
BssECI CCNNGG 6 cut(s) 806, 891, 1090, 1116, 1836, 2528
BssT1I CCWWGG 3 cut(s) 806, 1116, 2528
Bst2UI CCWGG 7 cut(s) 566, 893, 1092, 1575, 1832, 1968, 2192
Bst4CI ACNGT 5 cut(s) 161, 287, 526, 1138, 1257
Bst6I CTCTTC 4 cut(s) 106, 662, 1347, 2460
BstAPI GCANNNNNTGC 1 cut(s) 322
BstAUI TGTACA 2 cut(s) 718, 2805
BstBAI YACGTR 1 cut(s) 1298
BstDEI CTNAG 7 cut(s) 399, 1056, 1702, 1891, 2079, 2136, 2548
BstDSI CCRYGG 3 cut(s) 806, 1116, 1836
BstENI CCTNNNNNAGG 1 cut(s) 840
BstF5I GGATG 5 cut(s) 190, 799, 1543, 2006, 2538
BstFNI CGCG 2 cut(s) 395, 2249
BstHHI GCGC 2 cut(s) 397, 2249
BstMAI GTCTC 2 cut(s) 747, 1593
BstNI CCWGG 7 cut(s) 566, 893, 1092, 1575, 1832, 1968, 2192
BstNSI RCATGY 7 cut(s) 482, 775, 1676, 1775, 1816, 1906, 2426
BstSCI CCNGG 8 cut(s) 471, 564, 891, 1090, 1573, 1830, 1966, 2190
BstSFI CTRYAG 2 cut(s) 688, 1134
BstSLI GKGCMC 1 cut(s) 1180
BstUI CGCG 2 cut(s) 395, 2249
BstV1I GCAGC 7 cut(s) 1663, 1931, 2264, 2459, 2498, 2593, 2743
BstV2I GAAGAC 5 cut(s) 837, 954, 1815, 2174, 2397
BstX2I RGATCY 5 cut(s) 272, 304, 692, 1887, 2155
BstXI CCANNNNNNTGG 2 cut(s) 19, 382
BstYI RGATCY 5 cut(s) 272, 304, 692, 1887, 2155
Bsu36I CCTNAGG 2 cut(s) 399, 2548
BsuRI GGCC 7 cut(s) 460, 1229, 1666, 1835, 2047, 2347, 2691
BtgI CCRYGG 3 cut(s) 806, 1116, 1836
BtgZI GCGATG 1 cut(s) 2444
BtsCI GGATG 5 cut(s) 190, 799, 1543, 2006, 2538
BtsI GCAGTG 2 cut(s) 246, 2637
BtsIMutI CAGTG 3 cut(s) 246, 2376, 2637
BveI ACCTGC 2 cut(s) 1727, 2128
CaiI CAGNNNCTG 3 cut(s) 1097, 1934, 2135
CciI TCATGA 2 cut(s) 1168, 1800
CfoI GCGC 2 cut(s) 397, 2249
Cfr10I RCCGGY 1 cut(s) 2608
Cfr13I GGNCC 3 cut(s) 161, 459, 2046
CpoI CGGWCCG 1 cut(s) 161
CseI GACGC 4 cut(s) 401, 1448, 1940, 2123
CsiI ACCWGGT 1 cut(s) 1573
Csp6I GTAC 4 cut(s) 719, 2235, 2763, 2806
CspAI ACCGGT 1 cut(s) 2608
CspCI CAANNNNNGTGG 2 cut(s) 2196, 2231
CspI CGGWCCG 1 cut(s) 161
CviQI GTAC 4 cut(s) 719, 2235, 2763, 2806
DdeI CTNAG 7 cut(s) 399, 1056, 1702, 1891, 2079, 2136, 2548
DraIII CACNNNGTG 2 cut(s) 371, 1577
EaeI YGGCCR 1 cut(s) 2689
Eam1104I CTCTTC 4 cut(s) 106, 662, 1347, 2460
EarI CTCTTC 4 cut(s) 106, 662, 1347, 2460
EciI GGCGGA 1 cut(s) 690
Ecl136II GAGCTC 1 cut(s) 888
Eco130I CCWWGG 3 cut(s) 806, 1116, 2528
Eco147I AGGCCT 2 cut(s) 1666, 2347
Eco24I GRGCYC 1 cut(s) 890
Eco47I GGWCC 1 cut(s) 161
Eco53kI GAGCTC 1 cut(s) 888
Eco57I CTGAAG 4 cut(s) 849, 1881, 2227, 2511
Eco81I CCTNAGG 2 cut(s) 399, 2548
Eco88I CYCGRG 2 cut(s) 2052, 2219
EcoICRI GAGCTC 1 cut(s) 888
EcoNI CCTNNNNNAGG 1 cut(s) 840
EcoRII CCWGG 7 cut(s) 564, 891, 1090, 1573, 1830, 1966, 2190
EcoT14I CCWWGG 3 cut(s) 806, 1116, 2528
EcoT22I ATGCAT 6 cut(s) 588, 654, 1036, 1448, 1773, 1818
EcoT38I GRGCYC 1 cut(s) 890
ErhI CCWWGG 3 cut(s) 806, 1116, 2528
Esp3I CGTCTC 1 cut(s) 747
FalI AAGNNNNNCTT 2 cut(s) 1148, 1180
FaqI GGGAC 1 cut(s) 455
FauI CCCGC 1 cut(s) 469
FauNDI CATATG 2 cut(s) 1431, 2810
FbaI TGATCA 1 cut(s) 550
FokI GGATG 5 cut(s) 197, 806, 1550, 1993, 2545
FriOI GRGCYC 1 cut(s) 890
FspBI CTAG 7 cut(s) 405, 489, 534, 1008, 1515, 1662, 2789
GlaI GCGC 2 cut(s) 396, 2248
GsuI CTGGAG 4 cut(s) 771, 914, 1814, 1989
HaeIII GGCC 7 cut(s) 460, 1229, 1666, 1835, 2047, 2347, 2691
HapII CCGG 2 cut(s) 473, 2609
HgaI GACGC 4 cut(s) 401, 1448, 1940, 2123
HhaI GCGC 2 cut(s) 397, 2249
Hin6I GCGC 2 cut(s) 395, 2247
HinP1I GCGC 2 cut(s) 395, 2247
HindIII AAGCTT 2 cut(s) 410, 495
HpaII CCGG 2 cut(s) 473, 2609
HphI GGTGA 6 cut(s) 779, 912, 1214, 1589, 1742, 1862
Hpy166II GTNNAC 3 cut(s) 1178, 1404, 2628
Hpy8I GTNNAC 3 cut(s) 1178, 1404, 2628
Hpy99I CGWCG 2 cut(s) 68, 161
HpyAV CCTTC 4 cut(s) 587, 707, 1710, 2251
HpyCH4III ACNGT 5 cut(s) 161, 287, 526, 1138, 1257
HpyCH4IV ACGT 1 cut(s) 1297
HpyF3I CTNAG 7 cut(s) 399, 1056, 1702, 1891, 2079, 2136, 2548
HpySE526I ACGT 1 cut(s) 1297
HspAI GCGC 2 cut(s) 395, 2247
Ksp22I TGATCA 1 cut(s) 550
LmnI GCTCC 4 cut(s) 367, 893, 1833, 2549
Lsp1109I GCAGC 7 cut(s) 1663, 1931, 2264, 2459, 2498, 2593, 2743
LweI GCATC 6 cut(s) 175, 661, 721, 1021, 1455, 1734
MabI ACCWGGT 1 cut(s) 1573
MaeI CTAG 7 cut(s) 405, 489, 534, 1008, 1515, 1662, 2789
MaeII ACGT 1 cut(s) 1297
MaeIII GTNAC 8 cut(s) 248, 739, 767, 1356, 1850, 1987, 2504, 2590
MbiI CCGCTC 2 cut(s) 2001, 2742
MfeI CAATTG 3 cut(s) 429, 1392, 1463
MflI RGATCY 5 cut(s) 272, 304, 692, 1887, 2155
MhlI GDGCHC 4 cut(s) 890, 1155, 1180, 1209
MlyI GAGTC 6 cut(s) 1364, 1547, 1625, 1847, 2188, 2266
MmeI TCCRAC 1 cut(s) 1471
Mph1103I ATGCAT 6 cut(s) 588, 654, 1036, 1448, 1773, 1818
MroXI GAANNNNTTC 3 cut(s) 1164, 1476, 1720
MseI TTAA 6 cut(s) 219, 416, 971, 1373, 1860, 2863
MslI CAYNNNNRTG 3 cut(s) 81, 1173, 1409
MspA1I CMGCKG 3 cut(s) 378, 1015, 1727
MspI CCGG 2 cut(s) 473, 2609
MspR9I CCNGG 8 cut(s) 473, 566, 893, 1092, 1575, 1832, 1968, 2192
MunI CAATTG 3 cut(s) 429, 1392, 1463
Mva1269I GAATGC 5 cut(s) 507, 1267, 1773, 1910, 1960
MvaI CCWGG 7 cut(s) 566, 893, 1092, 1575, 1832, 1968, 2192
MvnI CGCG 2 cut(s) 395, 2249
NciI CCSGG 1 cut(s) 473
NcoI CCATGG 2 cut(s) 806, 1116
NdeI CATATG 2 cut(s) 1431, 2810
NheI GCTAGC 2 cut(s) 404, 1007
NlaIV GGNNCC 7 cut(s) 306, 570, 1794, 1829, 2048, 2157, 2818
NmuCI GTSAC 4 cut(s) 767, 1356, 1850, 2504
NsiI ATGCAT 6 cut(s) 588, 654, 1036, 1448, 1773, 1818
NspI RCATGY 7 cut(s) 482, 775, 1676, 1775, 1816, 1906, 2426
OliI CACNNNNGTG 1 cut(s) 1409
PaeI GCATGC 1 cut(s) 1775
PaeR7I CTCGAG 2 cut(s) 2052, 2219
PagI TCATGA 2 cut(s) 1168, 1800
PaqCI CACCTGC 1 cut(s) 2128
PceI AGGCCT 2 cut(s) 1666, 2347
PciI ACATGT 2 cut(s) 1902, 2422
PctI GAATGC 5 cut(s) 507, 1267, 1773, 1910, 1960
PdmI GAANNNNTTC 3 cut(s) 1164, 1476, 1720
PfeI GAWTC 5 cut(s) 23, 746, 977, 1241, 2266
PflMI CCANNNNNTGG 1 cut(s) 1837
PfoI TCCNGGA 1 cut(s) 1966
PinAI ACCGGT 1 cut(s) 2608
PleI GAGTC 6 cut(s) 1363, 1547, 1625, 1847, 2187, 2266
PpsI GAGTC 6 cut(s) 1363, 1547, 1625, 1847, 2187, 2266
Ppu21I YACGTR 1 cut(s) 1298
PscI ACATGT 2 cut(s) 1902, 2422
PsiI TTATAA 1 cut(s) 213
Psp124BI GAGCTC 1 cut(s) 890
Psp6I CCWGG 7 cut(s) 564, 891, 1090, 1573, 1830, 1966, 2190
PspGI CCWGG 7 cut(s) 564, 891, 1090, 1573, 1830, 1966, 2190
PspN4I GGNNCC 7 cut(s) 306, 570, 1794, 1829, 2048, 2157, 2818
PspPI GGNCC 3 cut(s) 161, 459, 2046
PspXI VCTCGAGB 2 cut(s) 2052, 2219
PstNI CAGNNNCTG 3 cut(s) 1097, 1934, 2135
PsuI RGATCY 5 cut(s) 272, 304, 692, 1887, 2155
PvuII CAGCTG 2 cut(s) 1015, 1727
RsaI GTAC 4 cut(s) 720, 2236, 2764, 2807
RsaNI GTAC 4 cut(s) 719, 2235, 2763, 2806
RseI CAYNNNNRTG 3 cut(s) 81, 1173, 1409
Rsr2I CGGWCCG 1 cut(s) 161
RsrII CGGWCCG 1 cut(s) 161
SacI GAGCTC 1 cut(s) 890
SaqAI TTAA 6 cut(s) 219, 416, 971, 1373, 1860, 2863
Sau96I GGNCC 3 cut(s) 161, 459, 2046
SchI GAGTC 6 cut(s) 1364, 1547, 1625, 1847, 2188, 2266
ScrFI CCNGG 8 cut(s) 473, 566, 893, 1092, 1575, 1832, 1968, 2192
SduI GDGCHC 4 cut(s) 890, 1155, 1180, 1209
SexAI ACCWGGT 1 cut(s) 1573
SfaNI GCATC 6 cut(s) 175, 661, 721, 1021, 1455, 1734
SfcI CTRYAG 2 cut(s) 688, 1134
Sfr274I CTCGAG 2 cut(s) 2052, 2219
SinI GGWCC 1 cut(s) 161
SlaI CTCGAG 2 cut(s) 2052, 2219
SmiMI CAYNNNNRTG 3 cut(s) 81, 1173, 1409
SmlI CTYRAG 4 cut(s) 1154, 2052, 2219, 2704
SmoI CTYRAG 4 cut(s) 1154, 2052, 2219, 2704
SphI GCATGC 1 cut(s) 1775
SseBI AGGCCT 2 cut(s) 1666, 2347
SspI AATATT 1 cut(s) 2499
SspMI CTAG 7 cut(s) 405, 489, 534, 1008, 1515, 1662, 2789
SstI GAGCTC 1 cut(s) 890
StuI AGGCCT 2 cut(s) 1666, 2347
StyD4I CCNGG 8 cut(s) 471, 564, 891, 1090, 1573, 1830, 1966, 2190
StyI CCWWGG 3 cut(s) 806, 1116, 2528
TaaI ACNGT 5 cut(s) 161, 287, 526, 1138, 1257
TaiI ACGT 1 cut(s) 1300
TaqI TCGA 7 cut(s) 882, 1219, 1330, 1449, 2053, 2220, 2310
TatI WGTACW 3 cut(s) 718, 2234, 2805
TauI GCSGC 4 cut(s) 1232, 2062, 2149, 2252
TfiI GAWTC 5 cut(s) 23, 746, 977, 1241, 2266
Tru1I TTAA 6 cut(s) 219, 416, 971, 1373, 1860, 2863
Tru9I TTAA 6 cut(s) 219, 416, 971, 1373, 1860, 2863
TscAI CASTG 3 cut(s) 246, 2383, 2644
TseFI GTSAC 4 cut(s) 767, 1356, 1850, 2504
TseI GCWGC 7 cut(s) 1676, 1919, 2252, 2447, 2511, 2581, 2756
Tsp45I GTSAC 4 cut(s) 767, 1356, 1850, 2504
TspGWI ACGGA 2 cut(s) 2492, 2822
TspRI CASTG 3 cut(s) 246, 2383, 2644
Van91I CCANNNNNTGG 1 cut(s) 1837
VneI GTGCAC 1 cut(s) 1176
VpaK11BI GGWCC 1 cut(s) 161
XagI CCTNNNNNAGG 1 cut(s) 840
XapI RAATTY 4 cut(s) 620, 913, 1475, 2089
XceI RCATGY 7 cut(s) 482, 775, 1676, 1775, 1816, 1906, 2426
XcmI CCANNNNNNNNNTGG 1 cut(s) 813
XhoI CTCGAG 2 cut(s) 2052, 2219
XmnI GAANNNNTTC 3 cut(s) 1164, 1476, 1720
XspI CTAG 7 cut(s) 405, 489, 534, 1008, 1515, 1662, 2789
Zsp2I ATGCAT 6 cut(s) 588, 654, 1036, 1448, 1773, 1818
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.