RchiOBHm_Chr5g0070931

Rab3 GTPase-activating protein catalytic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0004790
Physical Location & Seq
Forward (+)
76736174 .. 76744680
8507 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34610

Sequence Viewer

Length: 2868 bp
ATGGCTTCTTCCAGCAAGATGGAATCTTCCGCAGACCACCAAGCTGAACAGGAGGAGGAGGACGACGACGAGGTTGAGCATTTTGATGATTTCACTCTCGCCTCTTCATGGGAAAGGTTCATTTCTGATATAGAGGCGGTTTGTAGGCAATGGATAGCCGACGGTCCGAAAAATCTGTTGAAAAAGGATGCAAGGGAGGTTTCAGAGGAGTTTTATAAGGTTAAATATGAGTTGAAGTTTGCAGTGAAAAGTTACTGCATGGAATACTACTTTGGATCTAAAAATGACGGTAAAGTTATGGATTGGGATCCCAGATTGCACGATTTGCAGTTGTGCTTTGGGGTCAAAGATTTTTTGGTAATTGCTCCACAAAGTGCCAGCGGAGTGGTTCTTGACGCGCCTGAGGCTAGCAAGCTTTTAAGTGCAGTTGCAATTGCTTTATCAAATTGTTCAAGTTTGTGGCCCGCATTTGTCCCGGTGCATATTCCTGCTAGGAAAGCTTACATTGGCATTCAGAATATGGGAACAGTTTTCACTAGAAGATTTGAGGCTGATCATATTGGTAGCCAGGTTCCGGTAAAACTTATGCATTTGGAAGGATTATACGAGTTGTTTGTTTCTAAATTTGCCTACTCCACATTGGACTTTTCCATGCATCTCTTCAAAGTCCATTTTACTATGAAGCTAACCTACAGATCCCTTCCGCCTGATGACGATGATGTACAAAGAGCTGATGCTGATGTTACTGAATCTGGAGACGATCTTGGTGGTGACATGCACAATAGAACACAATGGGATGATGATTGTCCATGGAGTGAGTGGTATTCTGCTGAAGACCCTGTAAAAGGATTTGAGTTGGTTGCCTTGTGGTCTGAAAAGGTGGTTGAGAGCTCCCTGGAGATGGCTGAACTGGAAAATTCTTCACCCCATGAGGCCCAAAAATGGATATTATCTCCTAATATGTCTTCAACTTTAACTGATTCTTCCAAAGGAAACAGAATTGGATTTGCTAGCCAGCTGTGCCTTTTGGTTGATGCATTGAACAAGTCATTTGAGGCTCAGTTTATGGAAGATTTCGTGTCAGTTGAAAACCCAGGTTCTGATAATTTGAACTCCTCCATGGTTATACCTCCTCCTACAGTTATTGATCGTGTGCTCAAGGAACTCTTTCATGATGGTGCACAATTCCCCAGTTTTGCCTCTGGTGAGCACAAGACTTCTCGAATAAAGGCCGCACCTCTGGAATCCCTTTTTGCACAGTTTTGTCTGCATTCTCTTTGGTTTGGAAACTGCAATATACGTGCTATTTCTGTTATCTGGATAGAGTTTGTTCGAGAAGTTCGGTGGTGTTGGGAAGAGTCACAGCCATTGCCCTTAATGCCAGCCTTTGGTTCAATTGATCTGTCCACTTGTGTGATTTTCCAGAAACTTCATATGCTTGCTATATGCATCGAGAGGAAGCGTCAACTGAATGAAGAATTTCAAGATTGTGTTGGAAGCATGGATCATTCATCTCCTAGTATTGAGGAAGATGGTCTGGATGAGGGGTCAGAGGACTCATGTATTATGCAAACACCTGGTGAAAACCTTGATGGGAAACGAGACAGCCCTGCAATTCCAGATGATTTGCAAGACTCTAAACAGAGTGTATCAAGTCTTGGTGCTAGGCCTGAACATGCTGCAGTGTCTGCTGATCTAAAACCCTCAGATTGTATCAGAAGGGGTTCAGCTGGTGAGGCAGGTATGATGCTTCTGAAATCAAAACAGAGAATGCATGCACCATTCACGCAGGAGGCACCACTCATGACAGAAGACATGCATGAAGAACGGCTCCAGGCCGTGGAAGCCTTTGGTGACTCACTTAACTTTTCTGCTTTTTTGGAGAGAGAGATCTTAGCTTCAGACATGTCAGCATTCAAAGCAGCAAATCCAGACGCTGTTTTTGAAGATTTTATTCGCTGGCATTCTCCTGGAGATTGGGAGAGTGATGTTACAAAAGAGAGCGGATCATCCAATAATCCTGCAACAGAGGGTTCAAAAGATGACTGGCCCCCTCAAGGGTGGCTGTCAAAGAGAATGTCTGAGAGTGGGAATTTGTGGCGAAAGATTTGGAATGACGCACCTGCTTTGCCAGCTTCTGAGCAGAAGCCGCTAATGGATCCAAATAGAGAGGGAGAAAAGAGTCTTCATTACCTGGAAACCTTACGACCACATCAGTTGCTCGAGCAAATGATATGTACTGCCTTCAGCGCGGCAGCAGATACTTTGAATCAGACTCATTTTGGAGGCTTGAAGCAGATGAAAACCAAACTCGATCAACTTTACCTTACTATGACATCTGCATTGAGGCCTTTGCAAGCAAATCGGCTATCTGCTGACACTGATACTATTGAAGACATAAAACGGCTGTGTGGTGTTTTTGAACATGTTGAGAAGCTACTCACTATTGCAGCTTCTCTTCATCGCAAGTTCTTGCACGCCCCACGCCTCTCTGAAGCAATATTCCGTGACTGCTGCAACTTTTATTTTCCAAGGATGGGAACAAGCTCCTCAGGCGACAATACTCCAAAGGAGTTTGAGAAGCAGCAAATAGTAACGATGAACGAAAGACCGGTGGTGTCAAATATGTTTACGCCTCCCACTGCTAACCAGTCATGGCGAAAAGTTTTGAGCTTGGGCAATCTTCTCAACGGCCATGAACCCGTCTTAAGAGAGATCATCTTTTCCAAGCGTGATAAAGTGAGCGGAAATCACTATGCTGCCCGTACTCCAAATGCTTATCAAGATGAACTAGAAACATATAGAATGTACATATGTGGAACCTCAAATGACCTCCGTATAGCACTTTCTGTTGTTTCTTGTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rab3-GTPase_cat

Protein Analysis

955

Amino Acids

107.51

Weight (kDa)

4.92

Isoelectric Point (pI)

52.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rab3-GTPase_cat PF13890 573 - 733 2.3e-53 Rab3 GTPase-activating protein catalytic subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 216
AarI CACCTGC 1 cut(s) 2131
Acc36I ACCTGC 2 cut(s) 1730, 2131
AccB1I GGYRCC 1 cut(s) 1795
AccB7I CCANNNNNTGG 2 cut(s) 1388, 1840
AccBSI CCGCTC 2 cut(s) 2004, 2745
AccII CGCG 2 cut(s) 398, 2252
AclWI GGATC 8 cut(s) 283, 302, 315, 690, 1512, 2014, 2153, 2166
AcoI YGGCCR 1 cut(s) 2692
AcsI RAATTY 4 cut(s) 623, 916, 1478, 2092
AcuI CTGAAG 4 cut(s) 852, 1884, 2230, 2514
AdeI CACNNNGTG 2 cut(s) 374, 1580
AfaI GTAC 4 cut(s) 723, 2239, 2767, 2810
AflII CTTAAG 1 cut(s) 2707
AflIII ACRYGT 2 cut(s) 1905, 2425
AgeI ACCGGT 1 cut(s) 2611
AjnI CCWGG 7 cut(s) 567, 894, 1093, 1576, 1833, 1969, 2193
AjuI GAANNNNNNNTTGG 2 cut(s) 255, 287
AleI CACNNNNGTG 1 cut(s) 1412
Alw21I GWGCWC 4 cut(s) 893, 1158, 1183, 1212
Alw26I GTCTC 2 cut(s) 750, 1596
Alw44I GTGCAC 1 cut(s) 1179
AlwI GGATC 8 cut(s) 283, 302, 315, 690, 1512, 2014, 2153, 2166
AlwNI CAGNNNCTG 4 cut(s) 1100, 1688, 1937, 2138
Ama87I CYCGRG 1 cut(s) 2222
AoxI GGCC 8 cut(s) 461, 933, 1230, 1667, 1836, 2048, 2348, 2692
ApaLI GTGCAC 1 cut(s) 1179
ApeKI GCWGC 7 cut(s) 1679, 1922, 2255, 2450, 2514, 2584, 2759
ApoI RAATTY 4 cut(s) 623, 916, 1478, 2092
ArsI GACNNNNNNTTYG 4 cut(s) 1925, 1957, 2063, 2095
AsiGI ACCGGT 1 cut(s) 2611
Asp700I GAANNNNTTC 3 cut(s) 1167, 1479, 1723
AspLEI GCGC 2 cut(s) 400, 2252
AspS9I GGNCC 4 cut(s) 164, 462, 934, 2049
AsuC2I CCSGG 1 cut(s) 476
AsuHPI GGTGA 6 cut(s) 782, 915, 1217, 1592, 1745, 1865
AsuNHI GCTAGC 2 cut(s) 407, 1010
AvaI CYCGRG 1 cut(s) 2222
AvaII GGWCC 1 cut(s) 164
AxyI CCTNAGG 2 cut(s) 402, 2551
BaeGI GKGCMC 1 cut(s) 1183
BamHI GGATCC 2 cut(s) 307, 2158
BanI GGYRCC 1 cut(s) 1795
BanII GRGCYC 1 cut(s) 893
BarI GAAGNNNNNNTAC 2 cut(s) 674, 706
BbsI GAAGAC 5 cut(s) 840, 957, 1818, 2177, 2400
Bbv12I GWGCWC 4 cut(s) 893, 1158, 1183, 1212
BbvI GCAGC 7 cut(s) 1666, 1934, 2267, 2462, 2501, 2596, 2746
BccI CCATC 6 cut(s) 13, 895, 1169, 1526, 1586, 2530
BceAI ACGGC 4 cut(s) 1823, 1844, 2420, 2707
BcgI CGANNNNNNTGC 2 cut(s) 2345, 2379
BciT130I CCWGG 7 cut(s) 569, 896, 1095, 1578, 1835, 1971, 2195
BclI TGATCA 1 cut(s) 553
BcnI CCSGG 1 cut(s) 476
BcoDI GTCTC 2 cut(s) 750, 1596
BfaI CTAG 7 cut(s) 408, 492, 537, 1011, 1518, 1665, 2792
BfmI CTRYAG 3 cut(s) 691, 1137, 1680
BfrI CTTAAG 1 cut(s) 2707
BfuAI ACCTGC 2 cut(s) 1730, 2131
BglII AGATCT 1 cut(s) 1890
Bme1390I CCNGG 8 cut(s) 476, 569, 896, 1095, 1578, 1835, 1971, 2195
Bme18I GGWCC 1 cut(s) 164
BmeT110I CYCGRG 1 cut(s) 2222
BmgT120I GGNCC 4 cut(s) 164, 462, 934, 2049
BmiI GGNNCC 7 cut(s) 309, 573, 1797, 1832, 2051, 2160, 2821
BmrFI CCNGG 8 cut(s) 476, 569, 896, 1095, 1578, 1835, 1971, 2195
BmrI ACTGGG 1 cut(s) 1185
BmsI GCATC 6 cut(s) 178, 664, 724, 1024, 1458, 1737
BmtI GCTAGC 2 cut(s) 411, 1014
BmuI ACTGGG 1 cut(s) 1185
BpiI GAAGAC 5 cut(s) 840, 957, 1818, 2177, 2400
BpmI CTGGAG 4 cut(s) 774, 917, 1817, 1992
BpuEI CTTGAG 2 cut(s) 1142, 2040
BpuMI CCSGG 1 cut(s) 476
BsaAI YACGTR 1 cut(s) 1301
BsaBI GATNNNNATC 1 cut(s) 306
BsaJI CCNNGG 6 cut(s) 809, 894, 1093, 1119, 1839, 2531
BsaWI WCCGGW 2 cut(s) 574, 2611
Bse118I RCCGGY 1 cut(s) 2611
Bse1I ACTGG 4 cut(s) 915, 1191, 2051, 2650
Bse21I CCTNAGG 2 cut(s) 402, 2551
Bse3DI GCAATG 2 cut(s) 155, 1367
Bse8I GATNNNNATC 1 cut(s) 306
BseBI CCWGG 7 cut(s) 569, 896, 1095, 1578, 1835, 1971, 2195
BseDI CCNNGG 6 cut(s) 809, 894, 1093, 1119, 1839, 2531
BseGI GGATG 5 cut(s) 193, 802, 1546, 2009, 2541
BseJI GATNNNNATC 1 cut(s) 306
BseMI GCAATG 2 cut(s) 155, 1367
BseMII CTCAG 6 cut(s) 393, 1073, 1719, 2073, 2130, 2565
BseNI ACTGG 4 cut(s) 915, 1191, 2051, 2650
BseRI GAGGAG 6 cut(s) 68, 71, 221, 1105, 1122, 2539
BseSI GKGCMC 1 cut(s) 1183
BseXI GCAGC 7 cut(s) 1666, 1934, 2267, 2462, 2501, 2596, 2746
BsgI GTGCAG 1 cut(s) 444
Bsh1236I CGCG 2 cut(s) 398, 2252
BshFI GGCC 8 cut(s) 463, 935, 1232, 1669, 1838, 2050, 2350, 2694
BshNI GGYRCC 1 cut(s) 1795
BshTI ACCGGT 1 cut(s) 2611
BsiHKAI GWGCWC 4 cut(s) 893, 1158, 1183, 1212
BsiHKCI CYCGRG 1 cut(s) 2222
BsiSI CCGG 3 cut(s) 476, 575, 2612
BslFI GGGAC 1 cut(s) 458
BsmAI GTCTC 2 cut(s) 750, 1596
BsmBI CGTCTC 1 cut(s) 750
BsmFI GGGAC 1 cut(s) 458
BsmI GAATGC 5 cut(s) 510, 1270, 1776, 1913, 1963
BsnI GGCC 8 cut(s) 463, 935, 1232, 1669, 1838, 2050, 2350, 2694
BsoBI CYCGRG 1 cut(s) 2222
Bsp1286I GDGCHC 4 cut(s) 893, 1158, 1183, 1212
Bsp1407I TGTACA 2 cut(s) 721, 2808
Bsp19I CCATGG 2 cut(s) 809, 1119
BspANI GGCC 8 cut(s) 463, 935, 1232, 1669, 1838, 2050, 2350, 2694
BspCNI CTCAG 6 cut(s) 394, 1072, 1718, 2074, 2131, 2564
BspFNI CGCG 2 cut(s) 398, 2252
BspHI TCATGA 2 cut(s) 1171, 1803
BspLI GGNNCC 7 cut(s) 309, 573, 1797, 1832, 2051, 2160, 2821
BspMAI CTGCAG 1 cut(s) 1684
BspMI ACCTGC 2 cut(s) 1730, 2131
BspOI GCTAGC 2 cut(s) 411, 1014
BspPI GGATC 8 cut(s) 283, 302, 315, 690, 1512, 2014, 2153, 2166
BspT107I GGYRCC 1 cut(s) 1795
BspTI CTTAAG 1 cut(s) 2707
BsrBI CCGCTC 2 cut(s) 2004, 2745
BsrDI GCAATG 2 cut(s) 155, 1367
BsrFI RCCGGY 1 cut(s) 2611
BsrGI TGTACA 2 cut(s) 721, 2808
BsrI ACTGG 4 cut(s) 915, 1191, 2051, 2650
BssAI RCCGGY 1 cut(s) 2611
BssECI CCNNGG 6 cut(s) 809, 894, 1093, 1119, 1839, 2531
BssT1I CCWWGG 3 cut(s) 809, 1119, 2531
Bst2UI CCWGG 7 cut(s) 569, 896, 1095, 1578, 1835, 1971, 2195
Bst4CI ACNGT 5 cut(s) 164, 290, 529, 1141, 1260
Bst6I CTCTTC 4 cut(s) 109, 665, 1350, 2463
BstAFI CTTAAG 1 cut(s) 2707
BstAPI GCANNNNNTGC 2 cut(s) 325, 1688
BstAUI TGTACA 2 cut(s) 721, 2808
BstBAI YACGTR 1 cut(s) 1301
BstDEI CTNAG 7 cut(s) 402, 1059, 1705, 1894, 2082, 2139, 2551
BstDSI CCRYGG 3 cut(s) 809, 1119, 1839
BstENI CCTNNNNNAGG 1 cut(s) 843
BstF5I GGATG 5 cut(s) 193, 802, 1546, 2009, 2541
BstFNI CGCG 2 cut(s) 398, 2252
BstHHI GCGC 2 cut(s) 400, 2252
BstMAI GTCTC 2 cut(s) 750, 1596
BstNI CCWGG 7 cut(s) 569, 896, 1095, 1578, 1835, 1971, 2195
BstNSI RCATGY 6 cut(s) 778, 1679, 1778, 1819, 1909, 2429
BstSCI CCNGG 8 cut(s) 474, 567, 894, 1093, 1576, 1833, 1969, 2193
BstSFI CTRYAG 3 cut(s) 691, 1137, 1680
BstSLI GKGCMC 1 cut(s) 1183
BstUI CGCG 2 cut(s) 398, 2252
BstV1I GCAGC 7 cut(s) 1666, 1934, 2267, 2462, 2501, 2596, 2746
BstV2I GAAGAC 5 cut(s) 840, 957, 1818, 2177, 2400
BstX2I RGATCY 5 cut(s) 275, 307, 695, 1890, 2158
BstXI CCANNNNNNTGG 2 cut(s) 19, 385
BstYI RGATCY 5 cut(s) 275, 307, 695, 1890, 2158
Bsu36I CCTNAGG 2 cut(s) 402, 2551
BsuRI GGCC 8 cut(s) 463, 935, 1232, 1669, 1838, 2050, 2350, 2694
BtgI CCRYGG 3 cut(s) 809, 1119, 1839
BtgZI GCGATG 1 cut(s) 2447
BtsCI GGATG 5 cut(s) 193, 802, 1546, 2009, 2541
BtsI GCAGTG 3 cut(s) 249, 1689, 2640
BtsIMutI CAGTG 4 cut(s) 249, 1689, 2379, 2640
BveI ACCTGC 2 cut(s) 1730, 2131
CaiI CAGNNNCTG 4 cut(s) 1100, 1688, 1937, 2138
CciI TCATGA 2 cut(s) 1171, 1803
CfoI GCGC 2 cut(s) 400, 2252
Cfr10I RCCGGY 1 cut(s) 2611
Cfr13I GGNCC 4 cut(s) 164, 462, 934, 2049
CpoI CGGWCCG 1 cut(s) 164
CseI GACGC 4 cut(s) 404, 1451, 1943, 2126
CsiI ACCWGGT 1 cut(s) 1576
Csp6I GTAC 4 cut(s) 722, 2238, 2766, 2809
CspAI ACCGGT 1 cut(s) 2611
CspCI CAANNNNNGTGG 2 cut(s) 2199, 2234
CspI CGGWCCG 1 cut(s) 164
CviQI GTAC 4 cut(s) 722, 2238, 2766, 2809
DdeI CTNAG 7 cut(s) 402, 1059, 1705, 1894, 2082, 2139, 2551
DraIII CACNNNGTG 2 cut(s) 374, 1580
EaeI YGGCCR 1 cut(s) 2692
Eam1104I CTCTTC 4 cut(s) 109, 665, 1350, 2463
EarI CTCTTC 4 cut(s) 109, 665, 1350, 2463
EciI GGCGGA 1 cut(s) 693
Ecl136II GAGCTC 1 cut(s) 891
Eco130I CCWWGG 3 cut(s) 809, 1119, 2531
Eco147I AGGCCT 2 cut(s) 1669, 2350
Eco24I GRGCYC 1 cut(s) 893
Eco47I GGWCC 1 cut(s) 164
Eco53kI GAGCTC 1 cut(s) 891
Eco57I CTGAAG 4 cut(s) 852, 1884, 2230, 2514
Eco81I CCTNAGG 2 cut(s) 402, 2551
Eco88I CYCGRG 1 cut(s) 2222
EcoICRI GAGCTC 1 cut(s) 891
EcoNI CCTNNNNNAGG 1 cut(s) 843
EcoRII CCWGG 7 cut(s) 567, 894, 1093, 1576, 1833, 1969, 2193
EcoT14I CCWWGG 3 cut(s) 809, 1119, 2531
EcoT22I ATGCAT 6 cut(s) 591, 657, 1039, 1451, 1776, 1821
EcoT38I GRGCYC 1 cut(s) 893
ErhI CCWWGG 3 cut(s) 809, 1119, 2531
Esp3I CGTCTC 1 cut(s) 750
FalI AAGNNNNNCTT 2 cut(s) 1151, 1183
FaqI GGGAC 1 cut(s) 458
FauI CCCGC 1 cut(s) 472
FauNDI CATATG 2 cut(s) 1434, 2813
FbaI TGATCA 1 cut(s) 553
FokI GGATG 5 cut(s) 200, 809, 1553, 1996, 2548
FriOI GRGCYC 1 cut(s) 893
FspBI CTAG 7 cut(s) 408, 492, 537, 1011, 1518, 1665, 2792
GlaI GCGC 2 cut(s) 399, 2251
GsuI CTGGAG 4 cut(s) 774, 917, 1817, 1992
HaeIII GGCC 8 cut(s) 463, 935, 1232, 1669, 1838, 2050, 2350, 2694
HapII CCGG 3 cut(s) 476, 575, 2612
HgaI GACGC 4 cut(s) 404, 1451, 1943, 2126
HhaI GCGC 2 cut(s) 400, 2252
Hin6I GCGC 2 cut(s) 398, 2250
HinP1I GCGC 2 cut(s) 398, 2250
HincII GTYRAC 1 cut(s) 1466
HindII GTYRAC 1 cut(s) 1466
HindIII AAGCTT 2 cut(s) 413, 498
HpaII CCGG 3 cut(s) 476, 575, 2612
HphI GGTGA 6 cut(s) 782, 915, 1217, 1592, 1745, 1865
Hpy166II GTNNAC 4 cut(s) 1181, 1407, 1466, 2631
Hpy8I GTNNAC 4 cut(s) 1181, 1407, 1466, 2631
Hpy99I CGWCG 3 cut(s) 68, 71, 164
HpyAV CCTTC 4 cut(s) 590, 710, 1713, 2254
HpyCH4III ACNGT 5 cut(s) 164, 290, 529, 1141, 1260
HpyCH4IV ACGT 1 cut(s) 1300
HpyF3I CTNAG 7 cut(s) 402, 1059, 1705, 1894, 2082, 2139, 2551
HpySE526I ACGT 1 cut(s) 1300
HspAI GCGC 2 cut(s) 398, 2250
Ksp22I TGATCA 1 cut(s) 553
LmnI GCTCC 4 cut(s) 370, 896, 1836, 2552
Lsp1109I GCAGC 7 cut(s) 1666, 1934, 2267, 2462, 2501, 2596, 2746
LweI GCATC 6 cut(s) 178, 664, 724, 1024, 1458, 1737
MabI ACCWGGT 1 cut(s) 1576
MaeI CTAG 7 cut(s) 408, 492, 537, 1011, 1518, 1665, 2792
MaeII ACGT 1 cut(s) 1300
MaeIII GTNAC 8 cut(s) 251, 742, 770, 1359, 1853, 1990, 2507, 2593
MbiI CCGCTC 2 cut(s) 2004, 2745
MfeI CAATTG 2 cut(s) 432, 1395
MflI RGATCY 5 cut(s) 275, 307, 695, 1890, 2158
MhlI GDGCHC 4 cut(s) 893, 1158, 1183, 1212
MlyI GAGTC 6 cut(s) 1367, 1550, 1628, 1850, 2191, 2269
MmeI TCCRAC 1 cut(s) 1474
Mph1103I ATGCAT 6 cut(s) 591, 657, 1039, 1451, 1776, 1821
MroXI GAANNNNTTC 3 cut(s) 1167, 1479, 1723
MseI TTAA 7 cut(s) 222, 419, 974, 1376, 1863, 2708, 2866
MslI CAYNNNNRTG 3 cut(s) 84, 1176, 1412
MspA1I CMGCKG 3 cut(s) 381, 1018, 1730
MspCI CTTAAG 1 cut(s) 2707
MspI CCGG 3 cut(s) 476, 575, 2612
MspR9I CCNGG 8 cut(s) 476, 569, 896, 1095, 1578, 1835, 1971, 2195
MunI CAATTG 2 cut(s) 432, 1395
Mva1269I GAATGC 5 cut(s) 510, 1270, 1776, 1913, 1963
MvaI CCWGG 7 cut(s) 569, 896, 1095, 1578, 1835, 1971, 2195
MvnI CGCG 2 cut(s) 398, 2252
NciI CCSGG 1 cut(s) 476
NcoI CCATGG 2 cut(s) 809, 1119
NdeI CATATG 2 cut(s) 1434, 2813
NheI GCTAGC 2 cut(s) 407, 1010
NlaIV GGNNCC 7 cut(s) 309, 573, 1797, 1832, 2051, 2160, 2821
NmuCI GTSAC 4 cut(s) 770, 1359, 1853, 2507
NsiI ATGCAT 6 cut(s) 591, 657, 1039, 1451, 1776, 1821
NspI RCATGY 6 cut(s) 778, 1679, 1778, 1819, 1909, 2429
OliI CACNNNNGTG 1 cut(s) 1412
PaeI GCATGC 1 cut(s) 1778
PaeR7I CTCGAG 1 cut(s) 2222
PagI TCATGA 2 cut(s) 1171, 1803
PaqCI CACCTGC 1 cut(s) 2131
PceI AGGCCT 2 cut(s) 1669, 2350
PciI ACATGT 2 cut(s) 1905, 2425
PctI GAATGC 5 cut(s) 510, 1270, 1776, 1913, 1963
PdmI GAANNNNTTC 3 cut(s) 1167, 1479, 1723
PfeI GAWTC 5 cut(s) 23, 749, 980, 1244, 2269
PflMI CCANNNNNTGG 2 cut(s) 1388, 1840
PfoI TCCNGGA 1 cut(s) 1969
PinAI ACCGGT 1 cut(s) 2611
PleI GAGTC 6 cut(s) 1366, 1550, 1628, 1850, 2190, 2269
PpsI GAGTC 6 cut(s) 1366, 1550, 1628, 1850, 2190, 2269
Ppu21I YACGTR 1 cut(s) 1301
PscI ACATGT 2 cut(s) 1905, 2425
PsiI TTATAA 1 cut(s) 216
Psp124BI GAGCTC 1 cut(s) 893
Psp6I CCWGG 7 cut(s) 567, 894, 1093, 1576, 1833, 1969, 2193
PspGI CCWGG 7 cut(s) 567, 894, 1093, 1576, 1833, 1969, 2193
PspN4I GGNNCC 7 cut(s) 309, 573, 1797, 1832, 2051, 2160, 2821
PspPI GGNCC 4 cut(s) 164, 462, 934, 2049
PspXI VCTCGAGB 1 cut(s) 2222
PstI CTGCAG 1 cut(s) 1684
PstNI CAGNNNCTG 4 cut(s) 1100, 1688, 1937, 2138
PsuI RGATCY 5 cut(s) 275, 307, 695, 1890, 2158
PvuII CAGCTG 2 cut(s) 1018, 1730
RsaI GTAC 4 cut(s) 723, 2239, 2767, 2810
RsaNI GTAC 4 cut(s) 722, 2238, 2766, 2809
RseI CAYNNNNRTG 3 cut(s) 84, 1176, 1412
Rsr2I CGGWCCG 1 cut(s) 164
RsrII CGGWCCG 1 cut(s) 164
SacI GAGCTC 1 cut(s) 893
SaqAI TTAA 7 cut(s) 222, 419, 974, 1376, 1863, 2708, 2866
Sau96I GGNCC 4 cut(s) 164, 462, 934, 2049
SchI GAGTC 6 cut(s) 1367, 1550, 1628, 1850, 2191, 2269
ScrFI CCNGG 8 cut(s) 476, 569, 896, 1095, 1578, 1835, 1971, 2195
SduI GDGCHC 4 cut(s) 893, 1158, 1183, 1212
SexAI ACCWGGT 1 cut(s) 1576
SfaNI GCATC 6 cut(s) 178, 664, 724, 1024, 1458, 1737
SfcI CTRYAG 3 cut(s) 691, 1137, 1680
Sfr274I CTCGAG 1 cut(s) 2222
SinI GGWCC 1 cut(s) 164
SlaI CTCGAG 1 cut(s) 2222
SmiMI CAYNNNNRTG 3 cut(s) 84, 1176, 1412
SmlI CTYRAG 4 cut(s) 1157, 2055, 2222, 2707
SmoI CTYRAG 4 cut(s) 1157, 2055, 2222, 2707
SphI GCATGC 1 cut(s) 1778
SseBI AGGCCT 2 cut(s) 1669, 2350
SspI AATATT 1 cut(s) 2502
SspMI CTAG 7 cut(s) 408, 492, 537, 1011, 1518, 1665, 2792
SstI GAGCTC 1 cut(s) 893
StuI AGGCCT 2 cut(s) 1669, 2350
StyD4I CCNGG 8 cut(s) 474, 567, 894, 1093, 1576, 1833, 1969, 2193
StyI CCWWGG 3 cut(s) 809, 1119, 2531
TaaI ACNGT 5 cut(s) 164, 290, 529, 1141, 1260
TaiI ACGT 1 cut(s) 1303
TaqI TCGA 5 cut(s) 1222, 1333, 1452, 2223, 2313
TatI WGTACW 3 cut(s) 721, 2237, 2808
TauI GCSGC 3 cut(s) 1235, 2152, 2255
TfiI GAWTC 5 cut(s) 23, 749, 980, 1244, 2269
Tru1I TTAA 7 cut(s) 222, 419, 974, 1376, 1863, 2708, 2866
Tru9I TTAA 7 cut(s) 222, 419, 974, 1376, 1863, 2708, 2866
TscAI CASTG 4 cut(s) 249, 1689, 2386, 2647
TseFI GTSAC 4 cut(s) 770, 1359, 1853, 2507
TseI GCWGC 7 cut(s) 1679, 1922, 2255, 2450, 2514, 2584, 2759
Tsp45I GTSAC 4 cut(s) 770, 1359, 1853, 2507
TspGWI ACGGA 2 cut(s) 2495, 2825
TspRI CASTG 4 cut(s) 249, 1689, 2386, 2647
Van91I CCANNNNNTGG 2 cut(s) 1388, 1840
Vha464I CTTAAG 1 cut(s) 2707
VneI GTGCAC 1 cut(s) 1179
VpaK11BI GGWCC 1 cut(s) 164
XagI CCTNNNNNAGG 1 cut(s) 843
XapI RAATTY 4 cut(s) 623, 916, 1478, 2092
XceI RCATGY 6 cut(s) 778, 1679, 1778, 1819, 1909, 2429
XcmI CCANNNNNNNNNTGG 1 cut(s) 816
XhoI CTCGAG 1 cut(s) 2222
XmnI GAANNNNTTC 3 cut(s) 1167, 1479, 1723
XspI CTAG 7 cut(s) 408, 492, 537, 1011, 1518, 1665, 2792
Zsp2I ATGCAT 6 cut(s) 591, 657, 1039, 1451, 1776, 1821
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.