MD11G1090400.v1.1
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0015778
Physical Location & Seq
Reverse (-)
7509084 .. 7511910
2827 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1090400.v1.1.491

Sequence Viewer

Length: 726 bp
ATGTCTGCTCAAGGTTCACATGATCTTTCTCAACAAAGAAATGACTCACAACCACATGGGTTTTTGGCTGCTGATACATGGGTGTTGGACACTGGTGCCACATATCACATGACAGCCAATGTCAATACTCTTAATCAAGTCTCAGCTTATAATGGAGAAGAAAAGATAATCATAGGGAATGGTCAAGACAAGGTAACCAATGTGATTATCTACCAAGGAAAGAGTGATGGGGAGCTCTTTACCATACCTGTGACATTGTTTTCAAGATCAAATTCAAGGGATGGAGTAGCAAAGGAGTTAAGAGCTGGTTTTCTGGGAAAAATGGTAAAGACTGCAATTTGGCACAAGAGATTGGGTCATCCCTCTGAAGAGATACTAAAAGCTATGTTAAAGTCAGCTAAAGTACCAGTTACCATAGATTCTTCAGAGTCAGTATGTTCATCATGTATTTCAAGCAAAATGTGTAGGCAATCATTTCCTGTAAAACATAGTAGTGCAAGTCTTATGTTTGAAAGAGTACATTTAGATGTATGGGGACCTTCTCCAAAACTGTCAATTGAAGGATATAGGTATTACATTAGCTTCATTGATGAATATTCAAGGTTTCTGTGGATTTTTCCTCTGATTAATAAATCTGAAGCAGTGAATGCATTTGTGAAATTTCATGCGGTTGTGCTTAACCAGTTCAATACTTCCATTAAATGTTTACAAACTGATGGAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 Retrotran_gag_3 Retrotrans_gag gag_pre-integrs rve

Protein Analysis

242

Amino Acids

26.8

Weight (kDa)

8.61

Isoelectric Point (pI)

43.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
gag_pre-integrs PF13976 111 - 154 5.7e-11 GAG-pre-integrase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 150
AccB1I GGYRCC 1 cut(s) 95
AciI CCGC 1 cut(s) 668
AcsI RAATTY 2 cut(s) 271, 659
AcuI CTGAAG 3 cut(s) 387, 408, 657
AfaI GTAC 2 cut(s) 405, 519
AgsI TTSAA 7 cut(s) 264, 276, 453, 512, 560, 600, 688
AluBI AGCT 6 cut(s) 146, 235, 305, 383, 398, 582
AluI AGCT 6 cut(s) 146, 235, 305, 383, 398, 582
Alw21I GWGCWC 1 cut(s) 237
Alw26I GTCTC 1 cut(s) 145
ApeKI GCWGC 1 cut(s) 68
ApoI RAATTY 2 cut(s) 271, 659
AseI ATTAAT 1 cut(s) 627
AspS9I GGNCC 1 cut(s) 536
AvaII GGWCC 1 cut(s) 536
BaeI ACNNNNGTAYC 2 cut(s) 66, 99
BanI GGYRCC 1 cut(s) 95
BanII GRGCYC 1 cut(s) 237
Bbv12I GWGCWC 1 cut(s) 237
BbvI GCAGC 1 cut(s) 55
BccI CCATC 3 cut(s) 221, 275, 710
BcoDI GTCTC 1 cut(s) 145
BisI GCNGC 1 cut(s) 69
BlsI GCNGC 1 cut(s) 70
Bme18I GGWCC 1 cut(s) 536
BmgT120I GGNCC 1 cut(s) 536
BmiI GGNNCC 2 cut(s) 97, 537
BsaJI CCNNGG 1 cut(s) 214
Bse1I ACTGG 3 cut(s) 97, 407, 682
BseDI CCNNGG 1 cut(s) 214
BseGI GGATG 2 cut(s) 286, 358
BseMII CTCAG 1 cut(s) 156
BseNI ACTGG 3 cut(s) 97, 407, 682
BseXI GCAGC 1 cut(s) 55
BshNI GGYRCC 1 cut(s) 95
BsiHKAI GWGCWC 1 cut(s) 237
BslFI GGGAC 1 cut(s) 549
BsmAI GTCTC 1 cut(s) 145
BsmFI GGGAC 1 cut(s) 549
BsmI GAATGC 1 cut(s) 652
Bsp1286I GDGCHC 1 cut(s) 237
Bsp143I GATC 2 cut(s) 22, 266
BspACI CCGC 1 cut(s) 668
BspCNI CTCAG 1 cut(s) 155
BspLI GGNNCC 2 cut(s) 97, 537
BspT107I GGYRCC 1 cut(s) 95
BsrI ACTGG 3 cut(s) 97, 407, 682
BssECI CCNNGG 1 cut(s) 214
BssMI GATC 2 cut(s) 22, 266
BssT1I CCWWGG 1 cut(s) 214
Bst4CI ACNGT 1 cut(s) 552
Bst6I CTCTTC 1 cut(s) 363
BstAPI GCANNNNNTGC 1 cut(s) 647
BstDEI CTNAG 1 cut(s) 142
BstEII GGTNACC 1 cut(s) 193
BstF5I GGATG 2 cut(s) 286, 358
BstKTI GATC 2 cut(s) 25, 269
BstMAI GTCTC 1 cut(s) 145
BstMBI GATC 2 cut(s) 22, 266
BstMWI GCNNNNNNNGC 1 cut(s) 647
BstPI GGTNACC 1 cut(s) 193
BstV1I GCAGC 1 cut(s) 55
BtsCI GGATG 2 cut(s) 286, 358
BtsI GCAGTG 1 cut(s) 648
BtsIMutI CAGTG 2 cut(s) 90, 648
Cfr13I GGNCC 1 cut(s) 536
Csp6I GTAC 2 cut(s) 404, 518
CviAII CATG 6 cut(s) 20, 56, 78, 109, 444, 665
CviJI RGCY 8 cut(s) 68, 116, 146, 235, 305, 383, 398, 582
CviKI_1 RGCY 8 cut(s) 68, 116, 146, 235, 305, 383, 398, 582
CviQI GTAC 2 cut(s) 404, 518
DdeI CTNAG 1 cut(s) 142
DpnI GATC 2 cut(s) 24, 268
DpnII GATC 2 cut(s) 22, 266
Eam1104I CTCTTC 1 cut(s) 363
EarI CTCTTC 1 cut(s) 363
Ecl136II GAGCTC 1 cut(s) 235
Eco130I CCWWGG 1 cut(s) 214
Eco24I GRGCYC 1 cut(s) 237
Eco47I GGWCC 1 cut(s) 536
Eco53kI GAGCTC 1 cut(s) 235
Eco57I CTGAAG 3 cut(s) 387, 408, 657
Eco91I GGTNACC 1 cut(s) 193
EcoICRI GAGCTC 1 cut(s) 235
EcoO109I RGGNCCY 1 cut(s) 536
EcoO65I GGTNACC 1 cut(s) 193
EcoT14I CCWWGG 1 cut(s) 214
EcoT22I ATGCAT 1 cut(s) 652
EcoT38I GRGCYC 1 cut(s) 237
ErhI CCWWGG 1 cut(s) 214
FaeI CATG 6 cut(s) 23, 59, 81, 112, 447, 668
FaqI GGGAC 1 cut(s) 549
FatI CATG 6 cut(s) 19, 55, 77, 108, 443, 664
Fnu4HI GCNGC 1 cut(s) 69
FokI GGATG 2 cut(s) 293, 345
FriOI GRGCYC 1 cut(s) 237
Fsp4HI GCNGC 1 cut(s) 69
GluI GCNGC 1 cut(s) 69
Hin1II CATG 6 cut(s) 23, 59, 81, 112, 447, 668
HinfI GANTC 3 cut(s) 44, 419, 428
Hpy166II GTNNAC 2 cut(s) 17, 707
Hpy188I TCNGA 4 cut(s) 367, 427, 624, 637
Hpy188III TCNNGA 2 cut(s) 185, 264
Hpy8I GTNNAC 2 cut(s) 17, 707
HpyAV CCTTC 2 cut(s) 549, 554
HpyCH4III ACNGT 1 cut(s) 552
HpyCH4V TGCA 3 cut(s) 335, 497, 650
HpyF10VI GCNNNNNNNGC 1 cut(s) 647
HpyF3I CTNAG 1 cut(s) 142
Hsp92II CATG 6 cut(s) 23, 59, 81, 112, 447, 668
Kzo9I GATC 2 cut(s) 22, 266
LmnI GCTCC 1 cut(s) 232
LpnPI CCDG 7 cut(s) 78, 261, 291, 299, 420, 492, 695
Lsp1109I GCAGC 1 cut(s) 55
MaeIII GTNAC 3 cut(s) 193, 250, 409
MalI GATC 2 cut(s) 24, 268
MboI GATC 2 cut(s) 22, 266
MboII GAAGA 3 cut(s) 170, 380, 414
MfeI CAATTG 1 cut(s) 555
MhlI GDGCHC 1 cut(s) 237
MluCI AATT 4 cut(s) 271, 336, 555, 659
MlyI GAGTC 2 cut(s) 38, 437
MmeI TCCRAC 1 cut(s) 66
MnlI CCTC 3 cut(s) 373, 630, 713
Mph1103I ATGCAT 1 cut(s) 652
MseI TTAA 6 cut(s) 132, 299, 389, 627, 678, 699
MslI CAYNNNNRTG 3 cut(s) 248, 492, 525
MunI CAATTG 1 cut(s) 555
Mva1269I GAATGC 1 cut(s) 652
MwoI GCNNNNNNNGC 1 cut(s) 647
NdeII GATC 2 cut(s) 22, 266
NlaIII CATG 6 cut(s) 23, 59, 81, 112, 447, 668
NlaIV GGNNCC 2 cut(s) 97, 537
NmuCI GTSAC 1 cut(s) 250
NsiI ATGCAT 1 cut(s) 652
PctI GAATGC 1 cut(s) 652
PfeI GAWTC 1 cut(s) 419
PkrI GCNGC 1 cut(s) 70
PleI GAGTC 2 cut(s) 38, 436
PpsI GAGTC 2 cut(s) 38, 436
PpuMI RGGWCCY 1 cut(s) 536
PshBI ATTAAT 1 cut(s) 627
PsiI TTATAA 1 cut(s) 150
Psp124BI GAGCTC 1 cut(s) 237
Psp5II RGGWCCY 1 cut(s) 536
PspEI GGTNACC 1 cut(s) 193
PspN4I GGNNCC 2 cut(s) 97, 537
PspPI GGNCC 1 cut(s) 536
PspPPI RGGWCCY 1 cut(s) 536
RsaI GTAC 2 cut(s) 405, 519
RsaNI GTAC 2 cut(s) 404, 518
RseI CAYNNNNRTG 3 cut(s) 248, 492, 525
SacI GAGCTC 1 cut(s) 237
SaqAI TTAA 6 cut(s) 132, 299, 389, 627, 678, 699
SatI GCNGC 1 cut(s) 69
Sau3AI GATC 2 cut(s) 22, 266
Sau96I GGNCC 1 cut(s) 536
SchI GAGTC 2 cut(s) 38, 437
SduI GDGCHC 1 cut(s) 237
SinI GGWCC 1 cut(s) 536
SmiMI CAYNNNNRTG 3 cut(s) 248, 492, 525
SmlI CTYRAG 1 cut(s) 9
SmoI CTYRAG 1 cut(s) 9
Sse9I AATT 4 cut(s) 271, 336, 555, 659
SsiI CCGC 1 cut(s) 668
SspI AATATT 1 cut(s) 596
SstI GAGCTC 1 cut(s) 237
StyI CCWWGG 1 cut(s) 214
TaaI ACNGT 1 cut(s) 552
TasI AATT 4 cut(s) 271, 336, 555, 659
TatI WGTACW 1 cut(s) 517
TfiI GAWTC 1 cut(s) 419
Tru1I TTAA 6 cut(s) 132, 299, 389, 627, 678, 699
Tru9I TTAA 6 cut(s) 132, 299, 389, 627, 678, 699
TscAI CASTG 2 cut(s) 97, 648
TseFI GTSAC 1 cut(s) 250
TseI GCWGC 1 cut(s) 68
Tsp45I GTSAC 1 cut(s) 250
TspDTI ATGAA 4 cut(s) 429, 574, 606, 653
TspRI CASTG 2 cut(s) 97, 648
VpaK11BI GGWCC 1 cut(s) 536
VspI ATTAAT 1 cut(s) 627
XapI RAATTY 2 cut(s) 271, 659
Zsp2I ATGCAT 1 cut(s) 652
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.