pycom14g05450

transposition, RNA-mediated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0015778
Physical Location & Seq
Forward (+)
5302611 .. 5304258
1648 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g05450.3

Sequence Viewer

Length: 1005 bp
ATGAAGACAAAGGTTGACAAGGGGGAGTCTGCTGCCATGGCAGCAAGGGCCCGTGGATCATCTAGTGGGTCGAACCGAGAAGGGGTACAAAACTGGCCTGGATCAACTCGGCCGAGCCAGACAAGCCGGAATCGTCCACAAGGTAAGTGCCCACACTGTGGCCTGACGGGACACTCCAAGAGTCGTTGTTTTGAGCTGATTGGATATCCCAAGAATTGGGATAAGACCCGTGATCCTCGGTGCAACAAATCTCGAGCCTTCATTGCCGAAACCAAGAATGATTCGGATCAGATAGCGGACAAGGCATCTGCCATGATAGATGCGGCAGGAAGTGATGGTAAGGCACTCAGTACTTCTACTTCTGTTATGAATAATACATGGATAATTGATTCCGGAGCTACTGAACATATGACTTGTGATTCTAGACAGGTTCCATCACTAAAAACATCCACACAAACTGAGGTCAATGTTGCTAATGGTAATGCCGTCCCTGTTATTGGGGAAGGCGCTGTTTCCCTTTTCGATACCATGAAACTTGACACTGTTCTTGTGGACATCCGGATGCGGAAGACGATTGGTTATGGTATTAGAAGAGGGAAACTTTATTACTTGGAGTTGACCACCAGTAGTTCTAGCTTGCTGACTCAAGCTTTCTCCGTTGATGATTCTCAAGGGATTACTAATAAAGTGTCAGACATCTGGATGTGGCACCGGCGCCTTGGGCATGCTTCCTTTGGTTATCTGCATAAGTTATTTCCTAGTTTGTTTGTCAAGAATGATGTTTCTCAATTTAAGTGTGATGTTTGTGAAATGGCTAAGAGCCATCGAACCTCTTTTCCTCCCAGTTTAAATAAAAGTACCGTTCCTTTTATGATTGTGCATTCTGATGTATGGGGACCGTCAAAAGTAGCCACTCTTGGTGGTGCTCATTGGTTTGTTACTTTTATTGATGATTGTACCCGCATGACTTGGGTTATTTTGTTAAAGTCGAAGAGTGAAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Retrotran_gag_2

Protein Analysis

335

Amino Acids

36.78

Weight (kDa)

9.36

Isoelectric Point (pI)

28.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 708, 714
AccB7I CCANNNNNTGG 2 cut(s) 158, 216
AccIII TCCGGA 2 cut(s) 392, 558
AciI CCGC 4 cut(s) 296, 323, 565, 961
AclWI GGATC 4 cut(s) 64, 109, 227, 294
AcoI YGGCCR 1 cut(s) 110
AcyI GRCGYC 1 cut(s) 715
AdeI CACNNNGTG 1 cut(s) 158
AfaI GTAC 4 cut(s) 87, 352, 859, 958
AfiI CCNNNNNNNGG 4 cut(s) 82, 158, 216, 497
AjnI CCWGG 1 cut(s) 97
AloI GAACNNNNNNTCC 2 cut(s) 820, 852
AluBI AGCT 4 cut(s) 196, 398, 636, 650
AluI AGCT 4 cut(s) 196, 398, 636, 650
Alw21I GWGCWC 1 cut(s) 928
AlwI GGATC 4 cut(s) 64, 109, 227, 294
Ama87I CYCGRG 1 cut(s) 252
Aor13HI TCCGGA 2 cut(s) 392, 558
AoxI GGCC 4 cut(s) 48, 95, 110, 160
ApaI GGGCCC 1 cut(s) 52
ApeKI GCWGC 2 cut(s) 32, 41
AspLEI GCGC 2 cut(s) 509, 717
AspS9I GGNCC 3 cut(s) 48, 49, 896
AvaI CYCGRG 1 cut(s) 252
AvaII GGWCC 1 cut(s) 896
BaeGI GKGCMC 2 cut(s) 52, 152
BanI GGYRCC 2 cut(s) 708, 714
BanII GRGCYC 1 cut(s) 52
BarI GAAGNNNNNNTAC 2 cut(s) 343, 375
BbsI GAAGAC 2 cut(s) 11, 575
Bbv12I GWGCWC 1 cut(s) 928
BbvI GCAGC 2 cut(s) 19, 53
BccI CCATC 3 cut(s) 329, 442, 831
BceAI ACGGC 1 cut(s) 470
BciT130I CCWGG 1 cut(s) 99
BfaI CTAG 4 cut(s) 63, 423, 633, 759
BfoI RGCGCY 2 cut(s) 510, 718
BisI GCNGC 3 cut(s) 33, 42, 324
BlsI GCNGC 3 cut(s) 34, 43, 325
BmcAI AGTACT 1 cut(s) 352
Bme1390I CCNGG 1 cut(s) 99
Bme18I GGWCC 1 cut(s) 896
BmeT110I CYCGRG 1 cut(s) 252
BmgT120I GGNCC 3 cut(s) 48, 49, 896
BmiI GGNNCC 5 cut(s) 50, 432, 710, 716, 897
BmrFI CCNGG 1 cut(s) 99
BmrI ACTGGG 1 cut(s) 837
BmsI GCATC 3 cut(s) 310, 314, 552
BmuI ACTGGG 1 cut(s) 837
BpiI GAAGAC 2 cut(s) 11, 575
BpuEI CTTGAG 2 cut(s) 630, 654
BsaBI GATNNNNATC 1 cut(s) 285
BsaHI GRCGYC 1 cut(s) 715
BsaJI CCNNGG 4 cut(s) 36, 52, 236, 718
BsaWI WCCGGW 2 cut(s) 392, 558
Bsc4I CCNNNNNNNGG 4 cut(s) 82, 158, 216, 497
Bse118I RCCGGY 1 cut(s) 711
Bse1I ACTGG 3 cut(s) 98, 624, 843
Bse3DI GCAATG 1 cut(s) 261
Bse8I GATNNNNATC 1 cut(s) 285
BseAI TCCGGA 2 cut(s) 392, 558
BseBI CCWGG 1 cut(s) 99
BseDI CCNNGG 4 cut(s) 36, 52, 236, 718
BseGI GGATG 4 cut(s) 446, 555, 567, 708
BseJI GATNNNNATC 1 cut(s) 285
BseLI CCNNNNNNNGG 4 cut(s) 82, 158, 216, 497
BseMI GCAATG 1 cut(s) 261
BseMII CTCAG 2 cut(s) 361, 450
BseNI ACTGG 3 cut(s) 98, 624, 843
BseSI GKGCMC 2 cut(s) 52, 152
BseX3I CGGCCG 1 cut(s) 110
BseXI GCAGC 2 cut(s) 19, 53
Bsh1285I CGRYCG 1 cut(s) 113
BshFI GGCC 4 cut(s) 50, 97, 112, 162
BshNI GGYRCC 2 cut(s) 708, 714
BsiEI CGRYCG 1 cut(s) 113
BsiHKAI GWGCWC 1 cut(s) 928
BsiHKCI CYCGRG 1 cut(s) 252
BsiSI CCGG 4 cut(s) 127, 393, 559, 712
BslFI GGGAC 3 cut(s) 183, 473, 909
BslI CCNNNNNNNGG 4 cut(s) 82, 158, 216, 497
BsmFI GGGAC 3 cut(s) 183, 473, 909
BsmI GAATGC 1 cut(s) 880
BsnI GGCC 4 cut(s) 50, 97, 112, 162
BsoBI CYCGRG 1 cut(s) 252
Bsp120I GGGCCC 1 cut(s) 48
Bsp1286I GDGCHC 3 cut(s) 52, 152, 928
Bsp13I TCCGGA 2 cut(s) 392, 558
Bsp143I GATC 4 cut(s) 56, 101, 232, 286
Bsp19I CCATGG 1 cut(s) 36
BspACI CCGC 4 cut(s) 296, 323, 565, 961
BspANI GGCC 4 cut(s) 50, 97, 112, 162
BspCNI CTCAG 2 cut(s) 360, 451
BspEI TCCGGA 2 cut(s) 392, 558
BspLI GGNNCC 5 cut(s) 50, 432, 710, 716, 897
BspPI GGATC 4 cut(s) 64, 109, 227, 294
BspT107I GGYRCC 2 cut(s) 708, 714
BsrDI GCAATG 1 cut(s) 261
BsrFI RCCGGY 1 cut(s) 711
BsrI ACTGG 3 cut(s) 98, 624, 843
BssAI RCCGGY 1 cut(s) 711
BssECI CCNNGG 4 cut(s) 36, 52, 236, 718
BssMI GATC 4 cut(s) 56, 101, 232, 286
BssNI GRCGYC 1 cut(s) 715
BssT1I CCWWGG 2 cut(s) 36, 718
Bst2UI CCWGG 1 cut(s) 99
Bst4CI ACNGT 4 cut(s) 158, 544, 862, 900
Bst6I CTCTTC 2 cut(s) 586, 986
BstACI GRCGYC 1 cut(s) 715
BstC8I GCNNGC 2 cut(s) 638, 726
BstDEI CTNAG 3 cut(s) 347, 459, 816
BstDSI CCRYGG 2 cut(s) 36, 52
BstF5I GGATG 4 cut(s) 446, 555, 567, 708
BstH2I RGCGCY 2 cut(s) 510, 718
BstHHI GCGC 2 cut(s) 509, 717
BstKTI GATC 4 cut(s) 59, 104, 235, 289
BstMBI GATC 4 cut(s) 56, 101, 232, 286
BstMCI CGRYCG 1 cut(s) 113
BstMWI GCNNNNNNNGC 7 cut(s) 38, 41, 47, 123, 263, 302, 721
BstNI CCWGG 1 cut(s) 99
BstNSI RCATGY 1 cut(s) 728
BstSCI CCNGG 1 cut(s) 97
BstSLI GKGCMC 2 cut(s) 52, 152
BstV1I GCAGC 2 cut(s) 19, 53
BstV2I GAAGAC 2 cut(s) 11, 575
BstZI CGGCCG 1 cut(s) 110
BsuRI GGCC 4 cut(s) 50, 97, 112, 162
BtgI CCRYGG 2 cut(s) 36, 52
BtsCI GGATG 4 cut(s) 446, 555, 567, 708
BtsIMutI CAGTG 2 cut(s) 154, 540
Cac8I GCNNGC 2 cut(s) 638, 726
CfoI GCGC 2 cut(s) 509, 717
Cfr10I RCCGGY 1 cut(s) 711
Cfr13I GGNCC 3 cut(s) 48, 49, 896
Csp6I GTAC 4 cut(s) 86, 351, 858, 957
CviAII CATG 6 cut(s) 37, 313, 378, 529, 725, 964
CviQI GTAC 4 cut(s) 86, 351, 858, 957
DdeI CTNAG 3 cut(s) 347, 459, 816
DinI GGCGCC 1 cut(s) 716
DpnI GATC 4 cut(s) 58, 103, 234, 288
DpnII GATC 4 cut(s) 56, 101, 232, 286
DraI TTTAAA 1 cut(s) 849
DraIII CACNNNGTG 1 cut(s) 158
EaeI YGGCCR 1 cut(s) 110
EagI CGGCCG 1 cut(s) 110
Eam1104I CTCTTC 2 cut(s) 586, 986
EarI CTCTTC 2 cut(s) 586, 986
EclXI CGGCCG 1 cut(s) 110
Eco130I CCWWGG 2 cut(s) 36, 718
Eco24I GRGCYC 1 cut(s) 52
Eco32I GATATC 1 cut(s) 206
Eco47I GGWCC 1 cut(s) 896
Eco52I CGGCCG 1 cut(s) 110
Eco88I CYCGRG 1 cut(s) 252
EcoO109I RGGNCCY 1 cut(s) 48
EcoRII CCWGG 1 cut(s) 97
EcoRV GATATC 1 cut(s) 206
EcoT14I CCWWGG 2 cut(s) 36, 718
EcoT38I GRGCYC 1 cut(s) 52
EgeI GGCGCC 1 cut(s) 716
EheI GGCGCC 1 cut(s) 716
ErhI CCWWGG 2 cut(s) 36, 718
FaeI CATG 6 cut(s) 40, 316, 381, 532, 728, 967
FaqI GGGAC 3 cut(s) 183, 473, 909
FatI CATG 6 cut(s) 36, 312, 377, 528, 724, 963
FauI CCCGC 1 cut(s) 968
FauNDI CATATG 1 cut(s) 408
Fnu4HI GCNGC 3 cut(s) 33, 42, 324
FokI GGATG 4 cut(s) 433, 542, 574, 715
FriOI GRGCYC 1 cut(s) 52
Fsp4HI GCNGC 3 cut(s) 33, 42, 324
FspBI CTAG 4 cut(s) 63, 423, 633, 759
GlaI GCGC 2 cut(s) 508, 716
GluI GCNGC 3 cut(s) 33, 42, 324
HaeII RGCGCY 2 cut(s) 510, 718
HaeIII GGCC 4 cut(s) 50, 97, 112, 162
HapII CCGG 4 cut(s) 127, 393, 559, 712
HhaI GCGC 2 cut(s) 509, 717
Hin1I GRCGYC 1 cut(s) 715
Hin1II CATG 6 cut(s) 40, 316, 381, 532, 728, 967
Hin6I GCGC 2 cut(s) 507, 715
HinP1I GCGC 2 cut(s) 507, 715
HincII GTYRAC 2 cut(s) 16, 618
HindII GTYRAC 2 cut(s) 16, 618
HindIII AAGCTT 1 cut(s) 648
HinfI GANTC 8 cut(s) 26, 130, 181, 281, 389, 419, 643, 665
HpaII CCGG 4 cut(s) 127, 393, 559, 712
Hpy166II GTNNAC 4 cut(s) 16, 137, 553, 618
Hpy188I TCNGA 4 cut(s) 286, 291, 694, 886
Hpy188III TCNNGA 6 cut(s) 252, 393, 423, 559, 700, 772
Hpy8I GTNNAC 4 cut(s) 16, 137, 553, 618
HpyAV CCTTC 3 cut(s) 74, 268, 497
HpyCH4III ACNGT 4 cut(s) 158, 544, 862, 900
HpyCH4V TGCA 3 cut(s) 243, 745, 880
HpyF10VI GCNNNNNNNGC 7 cut(s) 38, 41, 47, 123, 263, 302, 721
HpyF3I CTNAG 3 cut(s) 347, 459, 816
Hsp92I GRCGYC 1 cut(s) 715
Hsp92II CATG 6 cut(s) 40, 316, 381, 532, 728, 967
HspAI GCGC 2 cut(s) 507, 715
KasI GGCGCC 1 cut(s) 714
Kpn2I TCCGGA 2 cut(s) 392, 558
Kzo9I GATC 4 cut(s) 56, 101, 232, 286
LmnI GCTCC 1 cut(s) 395
Lsp1109I GCAGC 2 cut(s) 19, 53
LweI GCATC 3 cut(s) 310, 314, 552
MaeI CTAG 4 cut(s) 63, 423, 633, 759
MaeIII GTNAC 1 cut(s) 937
MalI GATC 4 cut(s) 58, 103, 234, 288
MboI GATC 4 cut(s) 56, 101, 232, 286
MboII GAAGA 4 cut(s) 16, 580, 603, 1003
MhlI GDGCHC 3 cut(s) 52, 152, 928
MluCI AATT 3 cut(s) 214, 384, 788
Mly113I GGCGCC 1 cut(s) 715
MlyI GAGTC 3 cut(s) 35, 190, 637
MnlI CCTC 5 cut(s) 246, 454, 587, 841, 849
MroI TCCGGA 2 cut(s) 392, 558
MseI TTAA 3 cut(s) 792, 848, 983
MslI CAYNNNNRTG 3 cut(s) 560, 701, 885
MspI CCGG 4 cut(s) 127, 393, 559, 712
MspR9I CCNGG 1 cut(s) 99
Mva1269I GAATGC 1 cut(s) 880
MvaI CCWGG 1 cut(s) 99
MwoI GCNNNNNNNGC 7 cut(s) 38, 41, 47, 123, 263, 302, 721
NarI GGCGCC 1 cut(s) 715
NcoI CCATGG 1 cut(s) 36
NdeI CATATG 1 cut(s) 408
NdeII GATC 4 cut(s) 56, 101, 232, 286
NlaIII CATG 6 cut(s) 40, 316, 381, 532, 728, 967
NlaIV GGNNCC 5 cut(s) 50, 432, 710, 716, 897
NmeAIII GCCGAG 2 cut(s) 88, 138
NspI RCATGY 1 cut(s) 728
PaeI GCATGC 1 cut(s) 728
PaeR7I CTCGAG 1 cut(s) 252
PctI GAATGC 1 cut(s) 880
PfeI GAWTC 5 cut(s) 130, 281, 389, 419, 665
PflMI CCANNNNNTGG 2 cut(s) 158, 216
PkrI GCNGC 3 cut(s) 34, 43, 325
PleI GAGTC 3 cut(s) 34, 189, 637
PluTI GGCGCC 1 cut(s) 718
PpsI GAGTC 3 cut(s) 34, 189, 637
Psp6I CCWGG 1 cut(s) 97
PspGI CCWGG 1 cut(s) 97
PspN4I GGNNCC 5 cut(s) 50, 432, 710, 716, 897
PspOMI GGGCCC 1 cut(s) 48
PspPI GGNCC 3 cut(s) 48, 49, 896
RsaI GTAC 4 cut(s) 87, 352, 859, 958
RsaNI GTAC 4 cut(s) 86, 351, 858, 957
RseI CAYNNNNRTG 3 cut(s) 560, 701, 885
SaqAI TTAA 3 cut(s) 792, 848, 983
SatI GCNGC 3 cut(s) 33, 42, 324
Sau3AI GATC 4 cut(s) 56, 101, 232, 286
Sau96I GGNCC 3 cut(s) 48, 49, 896
ScaI AGTACT 1 cut(s) 352
SchI GAGTC 3 cut(s) 35, 190, 637
ScrFI CCNGG 1 cut(s) 99
SduI GDGCHC 3 cut(s) 52, 152, 928
SetI ASST 9 cut(s) 15, 145, 198, 400, 432, 465, 638, 652, 833
SfaNI GCATC 3 cut(s) 310, 314, 552
SfoI GGCGCC 1 cut(s) 716
Sfr274I CTCGAG 1 cut(s) 252
SgrAI CRCCGGYG 1 cut(s) 711
SinI GGWCC 1 cut(s) 896
SlaI CTCGAG 1 cut(s) 252
SmiMI CAYNNNNRTG 3 cut(s) 560, 701, 885
SmlI CTYRAG 3 cut(s) 252, 645, 669
SmoI CTYRAG 3 cut(s) 252, 645, 669
SphI GCATGC 1 cut(s) 728
Sse9I AATT 3 cut(s) 214, 384, 788
SsiI CCGC 4 cut(s) 296, 323, 565, 961
SspDI GGCGCC 1 cut(s) 714
SspMI CTAG 4 cut(s) 63, 423, 633, 759
StyD4I CCNGG 1 cut(s) 97
StyI CCWWGG 2 cut(s) 36, 718
TaaI ACNGT 4 cut(s) 158, 544, 862, 900
TaqI TCGA 5 cut(s) 71, 253, 522, 826, 989
TasI AATT 3 cut(s) 214, 384, 788
TatI WGTACW 1 cut(s) 350
TauI GCSGC 1 cut(s) 326
TfiI GAWTC 5 cut(s) 130, 281, 389, 419, 665
Tru1I TTAA 3 cut(s) 792, 848, 983
Tru9I TTAA 3 cut(s) 792, 848, 983
TscAI CASTG 2 cut(s) 161, 547
TseI GCWGC 2 cut(s) 32, 41
TspDTI ATGAA 4 cut(s) 17, 250, 383, 545
TspGWI ACGGA 1 cut(s) 646
TspRI CASTG 2 cut(s) 161, 547
Van91I CCANNNNNTGG 2 cut(s) 158, 216
VpaK11BI GGWCC 1 cut(s) 896
XbaI TCTAGA 1 cut(s) 422
XceI RCATGY 1 cut(s) 728
XhoI CTCGAG 1 cut(s) 252
XspI CTAG 4 cut(s) 63, 423, 633, 759
ZrmI AGTACT 1 cut(s) 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.