pycom12g11180
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0015778
Physical Location & Seq
Forward (+)
13344224 .. 13344990
767 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g11180.2

Sequence Viewer

Length: 465 bp
ATGCCGTGCTCACTACTGATTTTGCCCAAGGACTTGGTTTCGAAGGCGATGATTGGTGTGGGTAAGCGACGTGGCGATCTGTATTACCTTGTGGCTCTTACCTCTTCTCTCCCCACCCGTCAACCTCTCTGCAACATCATTACCATCCCCTCCTCCCTCTGGCATAGGCGTCTTGGTCACCCCTCCTCCACTCGTTTGCAAGCTTTAGCATCTAGCTCTCTTAATTGCACTTTTGATTCTAGTCATATTTGTGATGTTTGTCCGTTGGCAAAACAAACTCGTCAACCTTTTATTTTGAGTTCAATTTCATCAACTTTCCCTTTTGCTTTAATTCATTGTGATATTTGGGGCCCGAATCGTCAATCTTCTCTTTCTGGTGCTAATTATTTCTTAACCATTGTGGATGATTTTTCTCGCTTTATGTGGGTCTTCCTCATGAAACATAAATCCGAACTCAACAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 Retrotran_gag_3 Retrotrans_gag gag_pre-integrs rve

Protein Analysis

155

Amino Acids

17.26

Weight (kDa)

9.38

Isoelectric Point (pI)

75.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcyI GRCGYC 1 cut(s) 169
AfiI CCNNNNNNNGG 1 cut(s) 159
AgsI TTSAA 1 cut(s) 303
AjiI CACGTC 1 cut(s) 71
AluBI AGCT 2 cut(s) 203, 216
AluI AGCT 2 cut(s) 203, 216
Alw21I GWGCWC 1 cut(s) 11
AoxI GGCC 1 cut(s) 349
ApaI GGGCCC 1 cut(s) 353
AspS9I GGNCC 2 cut(s) 349, 350
AsuHPI GGTGA 1 cut(s) 170
AsuII TTCGAA 1 cut(s) 41
BaeGI GKGCMC 1 cut(s) 353
BanII GRGCYC 1 cut(s) 353
BbsI GAAGAC 1 cut(s) 421
Bbv12I GWGCWC 1 cut(s) 11
BccI CCATC 1 cut(s) 152
BfaI CTAG 2 cut(s) 213, 240
BmgBI CACGTC 1 cut(s) 71
BmgT120I GGNCC 2 cut(s) 349, 350
BmiI GGNNCC 2 cut(s) 350, 351
BmsI GCATC 1 cut(s) 218
BpiI GAAGAC 1 cut(s) 421
Bpu14I TTCGAA 1 cut(s) 41
BsaHI GRCGYC 1 cut(s) 169
BsaJI CCNNGG 1 cut(s) 27
BsaXI ACNNNNNCTCC 2 cut(s) 170, 200
Bsc4I CCNNNNNNNGG 1 cut(s) 159
BseDI CCNNGG 1 cut(s) 27
BseGI GGATG 2 cut(s) 144, 409
BseLI CCNNNNNNNGG 1 cut(s) 159
BseRI GAGGAG 2 cut(s) 142, 175
BseSI GKGCMC 1 cut(s) 353
BshFI GGCC 1 cut(s) 351
BsiHKAI GWGCWC 1 cut(s) 11
BslI CCNNNNNNNGG 1 cut(s) 159
BsnI GGCC 1 cut(s) 351
Bsp119I TTCGAA 1 cut(s) 41
Bsp120I GGGCCC 1 cut(s) 349
Bsp1286I GDGCHC 2 cut(s) 11, 353
Bsp143I GATC 1 cut(s) 76
BspANI GGCC 1 cut(s) 351
BspHI TCATGA 1 cut(s) 435
BspLI GGNNCC 2 cut(s) 350, 351
BspT104I TTCGAA 1 cut(s) 41
BssECI CCNNGG 1 cut(s) 27
BssMI GATC 1 cut(s) 76
BssNI GRCGYC 1 cut(s) 169
BssT1I CCWWGG 1 cut(s) 27
Bst6I CTCTTC 1 cut(s) 109
BstACI GRCGYC 1 cut(s) 169
BstBI TTCGAA 1 cut(s) 41
BstC8I GCNNGC 1 cut(s) 201
BstEII GGTNACC 1 cut(s) 176
BstF5I GGATG 2 cut(s) 144, 409
BstKTI GATC 1 cut(s) 79
BstMBI GATC 1 cut(s) 76
BstPI GGTNACC 1 cut(s) 176
BstSLI GKGCMC 1 cut(s) 353
BstV2I GAAGAC 1 cut(s) 421
BstXI CCANNNNNNTGG 1 cut(s) 34
BsuRI GGCC 1 cut(s) 351
BtgZI GCGATG 1 cut(s) 62
BtrI CACGTC 1 cut(s) 71
BtsCI GGATG 2 cut(s) 144, 409
Cac8I GCNNGC 1 cut(s) 201
CciI TCATGA 1 cut(s) 435
Cfr13I GGNCC 2 cut(s) 349, 350
CseI GACGC 1 cut(s) 158
CviAII CATG 1 cut(s) 436
CviJI RGCY 4 cut(s) 95, 203, 216, 351
CviKI_1 RGCY 4 cut(s) 95, 203, 216, 351
DpnI GATC 1 cut(s) 78
DpnII GATC 1 cut(s) 76
Eam1104I CTCTTC 1 cut(s) 109
EarI CTCTTC 1 cut(s) 109
Eco130I CCWWGG 1 cut(s) 27
Eco24I GRGCYC 1 cut(s) 353
Eco91I GGTNACC 1 cut(s) 176
EcoO109I RGGNCCY 1 cut(s) 349
EcoO65I GGTNACC 1 cut(s) 176
EcoT14I CCWWGG 1 cut(s) 27
EcoT38I GRGCYC 1 cut(s) 353
ErhI CCWWGG 1 cut(s) 27
FaeI CATG 1 cut(s) 439
FaiI YATR 5 cut(s) 165, 246, 422, 437, 444
FatI CATG 1 cut(s) 435
FokI GGATG 2 cut(s) 131, 416
FriOI GRGCYC 1 cut(s) 353
FspBI CTAG 2 cut(s) 213, 240
HaeIII GGCC 1 cut(s) 351
HgaI GACGC 1 cut(s) 158
Hin1I GRCGYC 1 cut(s) 169
Hin1II CATG 1 cut(s) 439
HincII GTYRAC 2 cut(s) 122, 284
HindII GTYRAC 2 cut(s) 122, 284
HindIII AAGCTT 1 cut(s) 201
HinfI GANTC 2 cut(s) 236, 355
HphI GGTGA 1 cut(s) 170
Hpy166II GTNNAC 2 cut(s) 122, 284
Hpy188I TCNGA 1 cut(s) 451
Hpy188III TCNNGA 1 cut(s) 436
Hpy8I GTNNAC 2 cut(s) 122, 284
Hpy99I CGWCG 1 cut(s) 72
HpyAV CCTTC 1 cut(s) 37
HpyCH4IV ACGT 1 cut(s) 70
HpyCH4V TGCA 3 cut(s) 132, 199, 228
HpySE526I ACGT 1 cut(s) 70
Hsp92I GRCGYC 1 cut(s) 169
Hsp92II CATG 1 cut(s) 439
Kzo9I GATC 1 cut(s) 76
LpnPI CCDG 2 cut(s) 145, 360
LweI GCATC 1 cut(s) 218
MaeI CTAG 2 cut(s) 213, 240
MaeII ACGT 1 cut(s) 70
MaeIII GTNAC 1 cut(s) 176
MalI GATC 1 cut(s) 78
MboI GATC 1 cut(s) 76
MboII GAAGA 3 cut(s) 96, 357, 421
MhlI GDGCHC 2 cut(s) 11, 353
MluCI AATT 5 cut(s) 223, 303, 330, 382, 460
MnlI CCTC 8 cut(s) 112, 135, 160, 163, 167, 193, 196, 443
MseI TTAA 4 cut(s) 222, 329, 392, 463
MslI CAYNNNNRTG 1 cut(s) 249
NdeII GATC 1 cut(s) 76
NlaIII CATG 1 cut(s) 439
NlaIV GGNNCC 2 cut(s) 350, 351
NmuCI GTSAC 1 cut(s) 176
NspV TTCGAA 1 cut(s) 41
PagI TCATGA 1 cut(s) 435
PfeI GAWTC 2 cut(s) 236, 355
PspEI GGTNACC 1 cut(s) 176
PspN4I GGNNCC 2 cut(s) 350, 351
PspOMI GGGCCC 1 cut(s) 349
PspPI GGNCC 2 cut(s) 349, 350
RseI CAYNNNNRTG 1 cut(s) 249
SaqAI TTAA 4 cut(s) 222, 329, 392, 463
Sau3AI GATC 1 cut(s) 76
Sau96I GGNCC 2 cut(s) 349, 350
SduI GDGCHC 2 cut(s) 11, 353
SetI ASST 7 cut(s) 73, 90, 104, 127, 205, 218, 289
SfaNI GCATC 1 cut(s) 218
SfuI TTCGAA 1 cut(s) 41
SmiMI CAYNNNNRTG 1 cut(s) 249
Sse9I AATT 5 cut(s) 223, 303, 330, 382, 460
SspMI CTAG 2 cut(s) 213, 240
StyI CCWWGG 1 cut(s) 27
TaiI ACGT 1 cut(s) 73
TaqI TCGA 1 cut(s) 41
TasI AATT 5 cut(s) 223, 303, 330, 382, 460
TfiI GAWTC 2 cut(s) 236, 355
Tru1I TTAA 4 cut(s) 222, 329, 392, 463
Tru9I TTAA 4 cut(s) 222, 329, 392, 463
TseFI GTSAC 1 cut(s) 176
Tsp45I GTSAC 1 cut(s) 176
TspDTI ATGAA 3 cut(s) 297, 323, 452
TspGWI ACGGA 1 cut(s) 252
XspI CTAG 2 cut(s) 213, 240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.