MD14G1120600.v1.1

transposition, RNA-mediated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0015778
Physical Location & Seq
Reverse (-)
19497955 .. 19500738
2784 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1120600.v1.1.491

Sequence Viewer

Length: 453 bp
ATGCATCTAATTCTATGTCTTATGTTTCCTTTCCTGGTAATGTTGGATTTCAAGGTTTTTCTCCTCCTCCAGAGTATTTTAGTGGCATTGCTCCTTCATTTCTACATCAAGGACAAGGTAACAGGGGTGATACTTTACAAAGGAAAGAGTAGCCAAAATGAGTTGTTTAAGATTCTTGTACATGTGTTTCCAACTTTACTTTCTCAAGGTGATTCTATTACTGCTGCATTCATGGGAAAAGCTGTCAAGACTTCTTTGTGGCATAATAGGTTTGGTCATCCTTCAAATGAGATTTTGGAAGCTATGCTTAGGGATTCTAATGTGTCTGTCTCTAGTGATGTCAATGCTCATGTTTGTTCACAATGTCTGAGTGGCAAAATGAGTAAACTGTCTTTTTCAGAGAAGTTAGATAGAGTTGATATTCCTTTTCATAAAGTTCATGCTAACATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

151

Amino Acids

16.92

Weight (kDa)

9.05

Isoelectric Point (pI)

46.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
gag_pre-integrs PF13976 80 - 127 3.3e-11 GAG-pre-integrase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 1 cut(s) 180
AflIII ACRYGT 2 cut(s) 181, 447
AgsI TTSAA 2 cut(s) 52, 285
AjnI CCWGG 1 cut(s) 33
AjuI GAANNNNNNNTTGG 2 cut(s) 184, 216
AluBI AGCT 2 cut(s) 242, 302
AluI AGCT 2 cut(s) 242, 302
Alw26I GTCTC 1 cut(s) 334
ApeKI GCWGC 1 cut(s) 224
AsuHPI GGTGA 2 cut(s) 139, 221
BbvI GCAGC 1 cut(s) 211
BciT130I CCWGG 1 cut(s) 35
BcoDI GTCTC 1 cut(s) 334
BfaI CTAG 1 cut(s) 333
BisI GCNGC 1 cut(s) 225
BlsI GCNGC 1 cut(s) 226
Bme1390I CCNGG 1 cut(s) 35
BmrFI CCNGG 1 cut(s) 35
BmsI GCATC 1 cut(s) 13
BpmI CTGGAG 1 cut(s) 53
Bpu10I CCTNAGC 1 cut(s) 308
BpuEI CTTGAG 1 cut(s) 189
Bse3DI GCAATG 1 cut(s) 86
BseBI CCWGG 1 cut(s) 35
BseGI GGATG 1 cut(s) 277
BseMI GCAATG 1 cut(s) 86
BseMII CTCAG 1 cut(s) 359
BseRI GAGGAG 2 cut(s) 53, 56
BseXI GCAGC 1 cut(s) 211
BsmAI GTCTC 1 cut(s) 334
BsmI GAATGC 1 cut(s) 227
Bsp1407I TGTACA 1 cut(s) 178
BspCNI CTCAG 1 cut(s) 360
BsrDI GCAATG 1 cut(s) 86
BsrGI TGTACA 1 cut(s) 178
Bst2UI CCWGG 1 cut(s) 35
Bst4CI ACNGT 1 cut(s) 390
BstAUI TGTACA 1 cut(s) 178
BstDEI CTNAG 2 cut(s) 308, 368
BstF5I GGATG 1 cut(s) 277
BstMAI GTCTC 1 cut(s) 334
BstNI CCWGG 1 cut(s) 35
BstNSI RCATGY 2 cut(s) 185, 451
BstSCI CCNGG 1 cut(s) 33
BstV1I GCAGC 1 cut(s) 211
BtsCI GGATG 1 cut(s) 277
Csp6I GTAC 1 cut(s) 179
CviAII CATG 5 cut(s) 182, 232, 350, 440, 448
CviJI RGCY 3 cut(s) 153, 242, 302
CviKI_1 RGCY 3 cut(s) 153, 242, 302
CviQI GTAC 1 cut(s) 179
DdeI CTNAG 2 cut(s) 308, 368
EcoRII CCWGG 1 cut(s) 33
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 5 cut(s) 185, 235, 353, 443, 451
FalI AAGNNNNNCTT 2 cut(s) 291, 323
FatI CATG 5 cut(s) 181, 231, 349, 439, 447
Fnu4HI GCNGC 1 cut(s) 225
FokI GGATG 1 cut(s) 264
Fsp4HI GCNGC 1 cut(s) 225
FspBI CTAG 1 cut(s) 333
GluI GCNGC 1 cut(s) 225
GsuI CTGGAG 1 cut(s) 53
Hin1II CATG 5 cut(s) 185, 235, 353, 443, 451
HinfI GANTC 3 cut(s) 172, 212, 314
HphI GGTGA 2 cut(s) 139, 221
Hpy166II GTNNAC 2 cut(s) 359, 386
Hpy188I TCNGA 2 cut(s) 369, 400
Hpy188III TCNNGA 2 cut(s) 70, 247
Hpy8I GTNNAC 2 cut(s) 359, 386
HpyAV CCTTC 2 cut(s) 104, 291
HpyCH4III ACNGT 1 cut(s) 390
HpyCH4V TGCA 2 cut(s) 4, 227
HpyF3I CTNAG 2 cut(s) 308, 368
Hsp92II CATG 5 cut(s) 185, 235, 353, 443, 451
LmnI GCTCC 1 cut(s) 96
LpnPI CCDG 4 cut(s) 20, 47, 83, 108
Lsp1109I GCAGC 1 cut(s) 211
LweI GCATC 1 cut(s) 13
MaeI CTAG 1 cut(s) 333
MaeIII GTNAC 1 cut(s) 118
MluCI AATT 1 cut(s) 9
MmeI TCCRAC 2 cut(s) 24, 215
MnlI CCTC 2 cut(s) 74, 77
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 1 cut(s) 168
MspR9I CCNGG 1 cut(s) 35
Mva1269I GAATGC 1 cut(s) 227
MvaI CCWGG 1 cut(s) 35
NlaIII CATG 5 cut(s) 185, 235, 353, 443, 451
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 2 cut(s) 185, 451
PciI ACATGT 2 cut(s) 181, 447
PctI GAATGC 1 cut(s) 227
PfeI GAWTC 3 cut(s) 172, 212, 314
PkrI GCNGC 1 cut(s) 226
PscI ACATGT 2 cut(s) 181, 447
Psp6I CCWGG 1 cut(s) 33
PspGI CCWGG 1 cut(s) 33
RsaI GTAC 1 cut(s) 180
RsaNI GTAC 1 cut(s) 179
SaqAI TTAA 1 cut(s) 168
SatI GCNGC 1 cut(s) 225
ScrFI CCNGG 1 cut(s) 35
SetI ASST 6 cut(s) 57, 120, 211, 244, 272, 304
SfaNI GCATC 1 cut(s) 13
SmlI CTYRAG 1 cut(s) 204
SmoI CTYRAG 1 cut(s) 204
Sse9I AATT 1 cut(s) 9
SspMI CTAG 1 cut(s) 333
StyD4I CCNGG 1 cut(s) 33
TaaI ACNGT 1 cut(s) 390
TasI AATT 1 cut(s) 9
TatI WGTACW 1 cut(s) 178
TfiI GAWTC 3 cut(s) 172, 212, 314
Tru1I TTAA 1 cut(s) 168
Tru9I TTAA 1 cut(s) 168
TseI GCWGC 1 cut(s) 224
TspDTI ATGAA 4 cut(s) 86, 220, 419, 428
XceI RCATGY 2 cut(s) 185, 451
XspI CTAG 1 cut(s) 333
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.