Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0012841

Physical Location & Seq

Genomic Coordinates Forward (+)

12390251 .. 12393614

Gene Structure 3364 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1134600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1110 bp

ATGGGAAATTTTGGAAGCACCACCTCCGGGGGTCGCCGGAGGCACCGCCCGCAACCCCAAATGTCTGGGGGCACTCATGTTTTTGCTGCTTCACCACCACCTCATGCATCTCAATATCCAAACGGACCAGACTCCAATCCACCCCAATACCATCAATACCCGTCTCTATATCCAAATGGATCAAACCCAAATCCGACCCAGTTCTTGCAATACCCCGGATTCCACCCGCCGCCGCCTGTGCCGGGTCCGTATCCGCAGTCTTTTCACGGCACCGGGGTCTACATGGGCCCCACTCCCGCCAATTGGGTTCACGGGCAGTTCCCTTTCGGTGCACCCCCTCCTCCCCCTCAGGGTGCTCCCTTTGTGGAACATCAGAAGACGGTCACCATTAAGAATGACGTGAATATCAAGAAAGACTCTTTGAGGGTTGAGCCGGATGAGGAGGATCCCGGGAGGTTCCTTGTTACTTTCACGTTTGATGCTGCTGCGCCCGGAAGCATGACAGTTATGTTTTTTGCTAAAGAAGATGTAGACTGTAAACTGATAGCAACAAGGGAAAGCTTGCTTAAATCTGTTACAGTGCCTTTTGAGAAAGGCCTTGGTCAAAAGTTTAGACAACCTTCCGGAACTGGGATCGACTTCTCAGTGTTTGAGGACATAGGGTTAACAAAAGAGGGTGACGTAGAAGTTTATCCTCTTGTGGTGAAGGCTGAATCCTTTCCCTTGAATGATGGTGAGCGGGAATCGGATGGAAATTCCGCTGGGAATTCTCAGATCACCCAGGCGGTGTTTGAAAAGAAGGAGAATGGGGAGTACAAAGTGCGGGTGATGAAGCAGATACTATGGGTGAATGGTACGAGGTACGAGCTGCAGGAGATCTTTGGTATTGGGAATTCAGTTGACAATGATCAAAATGATTCTGGCAAAGAATGTATAATTTGCTTGTCAGAACCTCGAGACACAACTGTCCTCCCATGCCGGCACATGTGCATGTGCAGTGGTTGTGCCAAGGTTCTGAGGTTCCAGACAAATAGATGCCCCGTCTGTAGGCAACCAGTTGATCGGCTCTTGGAAATCAAGGTGAGCAATGGGGCCAATAGTTCAGGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0008277 GO:0008589 GO:0009966 GO:0009968 GO:0009987 GO:0010646 GO:0010648 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0023051 GO:0023057 GO:0032446 GO:0036211 GO:0043170 GO:0043412 GO:0043949 GO:0043951 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0045744 GO:0045879 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0065007 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1902531 GO:1902532

KEGG Pathways

Metabolic & Signaling

ko04120 map04120

Pfam Domains

Protein Families

zf-C3HC4_3

Protein Analysis

370

Amino Acids

40.49

Weight (kDa)

6.16

Isoelectric Point (pI)

44.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNF157-like_N	PF26192	134 - 290	6e-67	RNF157-like N-terminal domain
zf-C3HC4_3	PF13920	309 - 355	4.7e-16	Zinc finger, C3HC4 type (RING finger)
zf-RING_2	PF13639	310 - 350	1.8e-06	Ring finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0012841)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G53410
fragaria_vesca	FvH4_3g32531 FvH4_3g32531
malus_domestica	MD03G1116400.v1.1 MD11G1134600.v1.1
prunus_persica	Prupe.6G100500_v2.0.a1
pyrus_communis	pycom11g11170
rosa_chinensis	RchiOBHm_Chr5g0059601
rosa_laevigata	RLG00000035307 RLG00000035309
rosa_multiflora	Rmu_ssc0000488.1_g000001
rosa_roxburghii	Rroxscaffold_1G00021010
rosa_rugosa	Rorug05G0323600
rosa_samantha	Rh5AG388600 Rh5BG400200 Rh5CG424500 Rh5DG416500
rosa_wichuraiana	Rw5G036590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	965
AccB1I	GGYRCC	2 cut(s)	42, 269
AccBSI	CCGCTC	1 cut(s)	739
AccI	GTMKAC	2 cut(s)	279, 531
AccIII	TCCGGA	1 cut(s)	623
AclWI	GGATC	4 cut(s)	187, 440, 453, 641
AcsI	RAATTY	4 cut(s)	7, 754, 766, 892
AfaI	GTAC	3 cut(s)	815, 856, 863
AfiI	CCNNNNNNNGG	6 cut(s)	27, 242, 303, 350, 630, 1047
AflIII	ACRYGT	1 cut(s)	984
AgsI	TTSAA	2 cut(s)	727, 794
AjiI	CACGTC	1 cut(s)	400
AjnI	CCWGG	1 cut(s)	780
AluBI	AGCT	2 cut(s)	561, 868
AluI	AGCT	2 cut(s)	561, 868
Alw21I	GWGCWC	2 cut(s)	334, 358
Alw26I	GTCTC	2 cut(s)	168, 951
Alw44I	GTGCAC	1 cut(s)	330
AlwI	GGATC	4 cut(s)	187, 440, 453, 641
Ama87I	CYCGRG	2 cut(s)	449, 954
Aor13HI	TCCGGA	1 cut(s)	623
AoxI	GGCC	3 cut(s)	286, 595, 1092
ApaI	GGGCCC	1 cut(s)	290
ApaLI	GTGCAC	1 cut(s)	330
ApeKI	GCWGC	4 cut(s)	86, 482, 485, 868
ApoI	RAATTY	4 cut(s)	7, 754, 766, 892
Asp700I	GAANNNNTTC	1 cut(s)	717
AspLEI	GCGC	1 cut(s)	490
AspS9I	GGNCC	5 cut(s)	125, 245, 286, 287, 1092
AsuC2I	CCSGG	7 cut(s)	28, 216, 243, 274, 450, 451, 492
AsuHPI	GGTGA	9 cut(s)	84, 376, 689, 715, 746, 769, 838, 859, 1093
AvaI	CYCGRG	2 cut(s)	449, 954
AvaII	GGWCC	2 cut(s)	125, 245
AxyI	CCTNAGG	1 cut(s)	348
BaeGI	GKGCMC	3 cut(s)	74, 290, 334
BamHI	GGATCC	1 cut(s)	445
BanI	GGYRCC	2 cut(s)	42, 269
BanII	GRGCYC	1 cut(s)	290
BbsI	GAAGAC	1 cut(s)	383
Bbv12I	GWGCWC	2 cut(s)	334, 358
BbvI	GCAGC	4 cut(s)	73, 469, 472, 855
BccI	CCATC	3 cut(s)	159, 725, 743
BceAI	ACGGC	1 cut(s)	283
BciT130I	CCWGG	1 cut(s)	782
BciVI	GTATCC	1 cut(s)	261
BclI	TGATCA	1 cut(s)	907
BcnI	CCSGG	7 cut(s)	28, 216, 243, 274, 450, 451, 492
BcoDI	GTCTC	2 cut(s)	168, 951
BfmI	CTRYAG	2 cut(s)	869, 1045
BfuI	GTATCC	1 cut(s)	261
BglII	AGATCT	1 cut(s)	876
BisI	GCNGC	6 cut(s)	87, 230, 233, 483, 486, 869
BlsI	GCNGC	6 cut(s)	88, 231, 234, 484, 487, 870
Bme1390I	CCNGG	8 cut(s)	28, 216, 243, 274, 450, 451, 492, 782
Bme18I	GGWCC	2 cut(s)	125, 245
BmeT110I	CYCGRG	2 cut(s)	449, 954
BmgBI	CACGTC	1 cut(s)	400
BmgT120I	GGNCC	5 cut(s)	125, 245, 286, 287, 1092
BmiI	GGNNCC	9 cut(s)	44, 246, 271, 288, 289, 447, 458, 1022, 1093
BmrFI	CCNGG	8 cut(s)	28, 216, 243, 274, 450, 451, 492, 782
BmrI	ACTGGG	2 cut(s)	193, 639
BmsI	GCATC	3 cut(s)	116, 469, 1025
BmuI	ACTGGG	2 cut(s)	193, 639
BpiI	GAAGAC	1 cut(s)	383
BpuMI	CCSGG	7 cut(s)	28, 216, 243, 274, 450, 451, 492
BsaJI	CCNNGG	7 cut(s)	27, 214, 273, 449, 598, 780, 1008
BsaWI	WCCGGW	1 cut(s)	623
Bsc4I	CCNNNNNNNGG	6 cut(s)	27, 242, 303, 350, 630, 1047
Bse118I	RCCGGY	1 cut(s)	978
Bse1I	ACTGG	3 cut(s)	199, 634, 1055
Bse21I	CCTNAGG	1 cut(s)	348
Bse3DI	GCAATG	1 cut(s)	1093
BseAI	TCCGGA	1 cut(s)	623
BseBI	CCWGG	1 cut(s)	782
BseDI	CCNNGG	7 cut(s)	27, 214, 273, 449, 598, 780, 1008
BseGI	GGATG	2 cut(s)	442, 754
BseLI	CCNNNNNNNGG	6 cut(s)	27, 242, 303, 350, 630, 1047
BseMI	GCAATG	1 cut(s)	1093
BseMII	CTCAG	4 cut(s)	362, 657, 785, 1007
BseNI	ACTGG	3 cut(s)	199, 634, 1055
BseRI	GAGGAG	2 cut(s)	330, 455
BseSI	GKGCMC	3 cut(s)	74, 290, 334
BseXI	GCAGC	4 cut(s)	73, 469, 472, 855
BseYI	CCCAGC	1 cut(s)	761
BsgI	GTGCAG	1 cut(s)	1015
BshFI	GGCC	3 cut(s)	288, 597, 1094
BshNI	GGYRCC	2 cut(s)	42, 269
BsiHKAI	GWGCWC	2 cut(s)	334, 358
BsiHKCI	CYCGRG	2 cut(s)	449, 954
BslI	CCNNNNNNNGG	6 cut(s)	27, 242, 303, 350, 630, 1047
BsmAI	GTCTC	2 cut(s)	168, 951
BsmBI	CGTCTC	1 cut(s)	168
BsnI	GGCC	3 cut(s)	288, 597, 1094
BsoBI	CYCGRG	2 cut(s)	449, 954
Bsp120I	GGGCCC	1 cut(s)	286
Bsp1286I	GDGCHC	4 cut(s)	74, 290, 334, 358
Bsp13I	TCCGGA	1 cut(s)	623
Bsp143I	GATC	7 cut(s)	179, 445, 633, 774, 876, 907, 1060
BspANI	GGCC	3 cut(s)	288, 597, 1094
BspCNI	CTCAG	4 cut(s)	361, 656, 784, 1008
BspEI	TCCGGA	1 cut(s)	623
BspLI	GGNNCC	9 cut(s)	44, 246, 271, 288, 289, 447, 458, 1022, 1093
BspMAI	CTGCAG	1 cut(s)	873
BspPI	GGATC	4 cut(s)	187, 440, 453, 641
BspT107I	GGYRCC	2 cut(s)	42, 269
BsrBI	CCGCTC	1 cut(s)	739
BsrDI	GCAATG	1 cut(s)	1093
BsrFI	RCCGGY	1 cut(s)	978
BsrI	ACTGG	3 cut(s)	199, 634, 1055
BssAI	RCCGGY	1 cut(s)	978
BssECI	CCNNGG	7 cut(s)	27, 214, 273, 449, 598, 780, 1008
BssMI	GATC	7 cut(s)	179, 445, 633, 774, 876, 907, 1060
BssT1I	CCWWGG	2 cut(s)	598, 1008
Bst2UI	CCWGG	1 cut(s)	782
Bst4CI	ACNGT	5 cut(s)	382, 505, 536, 580, 967
BstC8I	GCNNGC	3 cut(s)	50, 563, 980
BstDEI	CTNAG	4 cut(s)	348, 643, 771, 1016
BstEII	GGTNACC	1 cut(s)	382
BstF5I	GGATG	2 cut(s)	442, 754
BstHHI	GCGC	1 cut(s)	490
BstKTI	GATC	7 cut(s)	182, 448, 636, 777, 879, 910, 1063
BstMAI	GTCTC	2 cut(s)	168, 951
BstMBI	GATC	7 cut(s)	179, 445, 633, 774, 876, 907, 1060
BstMWI	GCNNNNNNNGC	2 cut(s)	49, 238
BstNI	CCWGG	1 cut(s)	782
BstNSI	RCATGY	2 cut(s)	988, 994
BstPI	GGTNACC	1 cut(s)	382
BstSCI	CCNGG	8 cut(s)	26, 214, 241, 272, 448, 449, 490, 780
BstSFI	CTRYAG	2 cut(s)	869, 1045
BstSLI	GKGCMC	3 cut(s)	74, 290, 334
BstV1I	GCAGC	4 cut(s)	73, 469, 472, 855
BstV2I	GAAGAC	1 cut(s)	383
BstX2I	RGATCY	2 cut(s)	445, 876
BstXI	CCANNNNNNTGG	1 cut(s)	65
BstYI	RGATCY	2 cut(s)	445, 876
Bsu36I	CCTNAGG	1 cut(s)	348
BsuI	GTATCC	1 cut(s)	261
BsuRI	GGCC	3 cut(s)	288, 597, 1094
BtrI	CACGTC	1 cut(s)	400
BtsCI	GGATG	2 cut(s)	442, 754
BtsI	GCAGTG	1 cut(s)	1003
BtsIMutI	CAGTG	3 cut(s)	585, 651, 1003
Cac8I	GCNNGC	3 cut(s)	50, 563, 980
CfoI	GCGC	1 cut(s)	490
Cfr10I	RCCGGY	1 cut(s)	978
Cfr13I	GGNCC	5 cut(s)	125, 245, 286, 287, 1092
Cfr9I	CCCGGG	1 cut(s)	449
Csp6I	GTAC	3 cut(s)	814, 855, 862
CviAII	CATG	7 cut(s)	77, 104, 283, 499, 975, 985, 991
CviJI	RGCY	8 cut(s)	288, 433, 561, 597, 710, 868, 1066, 1094
CviKI_1	RGCY	8 cut(s)	288, 433, 561, 597, 710, 868, 1066, 1094
CviQI	GTAC	3 cut(s)	814, 855, 862
DdeI	CTNAG	4 cut(s)	348, 643, 771, 1016
DpnI	GATC	7 cut(s)	181, 447, 635, 776, 878, 909, 1062
DpnII	GATC	7 cut(s)	179, 445, 633, 774, 876, 907, 1060
DrdI	GACNNNNNNGTC	1 cut(s)	965
DseDI	GACNNNNNNGTC	1 cut(s)	965
Eco130I	CCWWGG	2 cut(s)	598, 1008
Eco147I	AGGCCT	1 cut(s)	597
Eco24I	GRGCYC	1 cut(s)	290
Eco47I	GGWCC	2 cut(s)	125, 245
Eco81I	CCTNAGG	1 cut(s)	348
Eco88I	CYCGRG	2 cut(s)	449, 954
Eco91I	GGTNACC	1 cut(s)	382
EcoO109I	RGGNCCY	1 cut(s)	287
EcoO65I	GGTNACC	1 cut(s)	382
EcoRI	GAATTC	2 cut(s)	766, 892
EcoRII	CCWGG	1 cut(s)	780
EcoT14I	CCWWGG	2 cut(s)	598, 1008
EcoT22I	ATGCAT	1 cut(s)	109
EcoT38I	GRGCYC	1 cut(s)	290
ErhI	CCWWGG	2 cut(s)	598, 1008
Esp3I	CGTCTC	1 cut(s)	168
FaeI	CATG	7 cut(s)	80, 107, 286, 502, 978, 988, 994
FatI	CATG	7 cut(s)	76, 103, 282, 498, 974, 984, 990
FauI	CCCGC	5 cut(s)	57, 234, 304, 732, 816
FbaI	TGATCA	1 cut(s)	907
FblI	GTMKAC	2 cut(s)	279, 531
Fnu4HI	GCNGC	6 cut(s)	87, 230, 233, 483, 486, 869
FokI	GGATG	2 cut(s)	449, 761
FriOI	GRGCYC	1 cut(s)	290
Fsp4HI	GCNGC	6 cut(s)	87, 230, 233, 483, 486, 869
GlaI	GCGC	1 cut(s)	489
GluI	GCNGC	6 cut(s)	87, 230, 233, 483, 486, 869
GsaI	CCCAGC	1 cut(s)	765
HaeIII	GGCC	3 cut(s)	288, 597, 1094
HhaI	GCGC	1 cut(s)	490
Hin1II	CATG	7 cut(s)	80, 107, 286, 502, 978, 988, 994
Hin6I	GCGC	1 cut(s)	488
HinP1I	GCGC	1 cut(s)	488
HincII	GTYRAC	2 cut(s)	666, 901
HindII	GTYRAC	2 cut(s)	666, 901
HindIII	AAGCTT	1 cut(s)	559
HinfI	GANTC	6 cut(s)	131, 219, 416, 713, 743, 917
HpaI	GTTAAC	1 cut(s)	666
HphI	GGTGA	9 cut(s)	84, 376, 689, 715, 746, 769, 838, 859, 1093
Hpy166II	GTNNAC	7 cut(s)	280, 310, 332, 532, 539, 666, 901
Hpy188I	TCNGA	6 cut(s)	195, 375, 748, 774, 949, 1017
Hpy188III	TCNNGA	5 cut(s)	409, 624, 956, 1024, 1104
Hpy8I	GTNNAC	7 cut(s)	280, 310, 332, 532, 539, 666, 901
HpyAV	CCTTC	3 cut(s)	630, 700, 793
HpyCH4III	ACNGT	5 cut(s)	382, 505, 536, 580, 967
HpyCH4IV	ACGT	3 cut(s)	399, 473, 681
HpyCH4V	TGCA	6 cut(s)	107, 208, 332, 871, 990, 996
HpyF10VI	GCNNNNNNNGC	2 cut(s)	49, 238
HpyF3I	CTNAG	4 cut(s)	348, 643, 771, 1016
HpySE526I	ACGT	3 cut(s)	399, 473, 681
Hsp92II	CATG	7 cut(s)	80, 107, 286, 502, 978, 988, 994
HspAI	GCGC	1 cut(s)	488
Kpn2I	TCCGGA	1 cut(s)	623
KroI	GCCGGC	1 cut(s)	978
KroNI	GCCGGC	1 cut(s)	980
Ksp22I	TGATCA	1 cut(s)	907
KspAI	GTTAAC	1 cut(s)	666
Kzo9I	GATC	7 cut(s)	179, 445, 633, 774, 876, 907, 1060
LmnI	GCTCC	1 cut(s)	361
Lsp1109I	GCAGC	4 cut(s)	73, 469, 472, 855
LweI	GCATC	3 cut(s)	116, 469, 1025
MaeII	ACGT	3 cut(s)	399, 473, 681
MaeIII	GTNAC	4 cut(s)	382, 463, 574, 677
MalI	GATC	7 cut(s)	181, 447, 635, 776, 878, 909, 1062
MbiI	CCGCTC	1 cut(s)	739
MboI	GATC	7 cut(s)	179, 445, 633, 774, 876, 907, 1060
MboII	GAAGA	2 cut(s)	388, 536
MfeI	CAATTG	1 cut(s)	301
MflI	RGATCY	2 cut(s)	445, 876
MhlI	GDGCHC	4 cut(s)	74, 290, 334, 358
MluCI	AATT	6 cut(s)	7, 301, 754, 766, 892, 936
MlyI	GAGTC	2 cut(s)	125, 410
MmeI	TCCRAC	1 cut(s)	218
Mph1103I	ATGCAT	1 cut(s)	109
MroI	TCCGGA	1 cut(s)	623
MroNI	GCCGGC	1 cut(s)	978
MroXI	GAANNNNTTC	1 cut(s)	717
MseI	TTAA	3 cut(s)	390, 567, 665
MslI	CAYNNNNRTG	1 cut(s)	989
MspA1I	CMGCKG	1 cut(s)	761
MspR9I	CCNGG	8 cut(s)	28, 216, 243, 274, 450, 451, 492, 782
MunI	CAATTG	1 cut(s)	301
MvaI	CCWGG	1 cut(s)	782
MwoI	GCNNNNNNNGC	2 cut(s)	49, 238
NaeI	GCCGGC	1 cut(s)	980
NciI	CCSGG	7 cut(s)	28, 216, 243, 274, 450, 451, 492
NdeII	GATC	7 cut(s)	179, 445, 633, 774, 876, 907, 1060
NgoMIV	GCCGGC	1 cut(s)	978
NlaIII	CATG	7 cut(s)	80, 107, 286, 502, 978, 988, 994
NlaIV	GGNNCC	9 cut(s)	44, 246, 271, 288, 289, 447, 458, 1022, 1093
NmuCI	GTSAC	2 cut(s)	382, 677
NsiI	ATGCAT	1 cut(s)	109
NspI	RCATGY	2 cut(s)	988, 994
PaeR7I	CTCGAG	1 cut(s)	954
PceI	AGGCCT	1 cut(s)	597
PciI	ACATGT	1 cut(s)	984
PdiI	GCCGGC	1 cut(s)	980
PdmI	GAANNNNTTC	1 cut(s)	717
PfeI	GAWTC	4 cut(s)	219, 713, 743, 917
PkrI	GCNGC	6 cut(s)	88, 231, 234, 484, 487, 870
PleI	GAGTC	2 cut(s)	125, 410
PpsI	GAGTC	2 cut(s)	125, 410
PscI	ACATGT	1 cut(s)	984
Psp6I	CCWGG	1 cut(s)	780
PspEI	GGTNACC	1 cut(s)	382
PspFI	CCCAGC	1 cut(s)	761
PspGI	CCWGG	1 cut(s)	780
PspN4I	GGNNCC	9 cut(s)	44, 246, 271, 288, 289, 447, 458, 1022, 1093
PspOMI	GGGCCC	1 cut(s)	286
PspPI	GGNCC	5 cut(s)	125, 245, 286, 287, 1092
PstI	CTGCAG	1 cut(s)	873
PsuI	RGATCY	2 cut(s)	445, 876
RsaI	GTAC	3 cut(s)	815, 856, 863
RsaNI	GTAC	3 cut(s)	814, 855, 862
RseI	CAYNNNNRTG	1 cut(s)	989
SaqAI	TTAA	3 cut(s)	390, 567, 665
SatI	GCNGC	6 cut(s)	87, 230, 233, 483, 486, 869
Sau3AI	GATC	7 cut(s)	179, 445, 633, 774, 876, 907, 1060
Sau96I	GGNCC	5 cut(s)	125, 245, 286, 287, 1092
SchI	GAGTC	2 cut(s)	125, 410
ScrFI	CCNGG	8 cut(s)	28, 216, 243, 274, 450, 451, 492, 782
SduI	GDGCHC	4 cut(s)	74, 290, 334, 358
SfaNI	GCATC	3 cut(s)	116, 469, 1025
SfcI	CTRYAG	2 cut(s)	869, 1045
Sfr274I	CTCGAG	1 cut(s)	954
SinI	GGWCC	2 cut(s)	125, 245
SlaI	CTCGAG	1 cut(s)	954
SmaI	CCCGGG	1 cut(s)	451
SmiMI	CAYNNNNRTG	1 cut(s)	989
SmlI	CTYRAG	1 cut(s)	954
SmoI	CTYRAG	1 cut(s)	954
Sse9I	AATT	6 cut(s)	7, 301, 754, 766, 892, 936
SseBI	AGGCCT	1 cut(s)	597
StuI	AGGCCT	1 cut(s)	597
StyD4I	CCNGG	8 cut(s)	26, 214, 241, 272, 448, 449, 490, 780
StyI	CCWWGG	2 cut(s)	598, 1008
TaaI	ACNGT	5 cut(s)	382, 505, 536, 580, 967
TaiI	ACGT	3 cut(s)	402, 476, 684
TaqI	TCGA	2 cut(s)	636, 955
TasI	AATT	6 cut(s)	7, 301, 754, 766, 892, 936
TatI	WGTACW	1 cut(s)	813
TauI	GCSGC	2 cut(s)	232, 235
TfiI	GAWTC	4 cut(s)	219, 713, 743, 917
Tru1I	TTAA	3 cut(s)	390, 567, 665
Tru9I	TTAA	3 cut(s)	390, 567, 665
TscAI	CASTG	3 cut(s)	585, 651, 1003
TseFI	GTSAC	2 cut(s)	382, 677
TseI	GCWGC	4 cut(s)	86, 482, 485, 868
Tsp45I	GTSAC	2 cut(s)	382, 677
TspDTI	ATGAA	1 cut(s)	845
TspGWI	ACGGA	2 cut(s)	138, 237
TspMI	CCCGGG	1 cut(s)	449
TspRI	CASTG	3 cut(s)	585, 651, 1003
VneI	GTGCAC	1 cut(s)	330
VpaK11BI	GGWCC	2 cut(s)	125, 245
XapI	RAATTY	4 cut(s)	7, 754, 766, 892
XceI	RCATGY	2 cut(s)	988, 994
XhoI	CTCGAG	1 cut(s)	954
XmaI	CCCGGG	1 cut(s)	449
XmiI	GTMKAC	2 cut(s)	279, 531
XmnI	GAANNNNTTC	1 cut(s)	717
Zsp2I	ATGCAT	1 cut(s)	109

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.