RLG00000035307

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0012841
Physical Location & Seq
Reverse (-)
64753094 .. 64755212
2119 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035307

Sequence Viewer

Length: 1065 bp
ATGTCGTCAACTGACCCGATCTTTCGGCCCAAAAGGAAAAAGATTATAGTGTGGGCCTGGATCCAGGTCCATGATGTTTGGGTCGGGTCCGATACTAGTTTTTTCACAACGGCTTTAACTGACCACCCCTCTGTAACTGAAATCCAAAACCCACAATCTCCAATCAACGGTCACGATTCCACGGATCAACCGCAACCCCCGCAGGACCCGGAAACCCAGCCGCCGCAAGACGACGCCGTTTCGGGAGACAAGCTGAAGCGGGAGGAGGAAGGAAGCGATAAGAAGCCGGACGCCGCCGGGCATCACCCTCCGTGGAAGACGAGCCTCTGCTCCTTCTTCCGCCGCCAGGACGCGTCCTGTGGCCAGGCAGCGGGTGCCGCTATGCCCACAGTGAAGCGGAGCTCCGGCCCCGACCCGATAACACTTGTGCTTTGGTGCTACAGCATTACCGTCATGTTTTTTACGAAAGAAGATGCAGACTTGAACTTGATAGCAACAAAGGAAAGCTTGCTTAAACCTGTTAAAGTGCCGTTTGACAAAGGTCTTGGTCAAAAGTTTAGACAACCTTCTGGAACTGGGATCGACTTTTCAATGTTTGAGGAGATAGGGTTGACAAAAGACGGTGACATAGAAGTTTATCCTCTTGTGGTGAAGGCTGAAGCACATCTCGTGAATGAAGGTGAACGTGAATCAGAGGGAAATTCCACCGGGAACTCTCAGATCACACAGGCAGTGTTTGAAAAGAAGGAGAATGGTGAGTACAAAGTGCGGGTGGTGAAACAGATACTATGGGTGGATAAAATGAGGTATGAGCTGCAGGAGATCTATGGTATTGGGAATGCAGTTGAAGGTGATGAAAATGATAATGAAGCTGGAAAAGACTGTAAAATTTGCTTGTCAGAACCTCGAGATACAACTGTGCTCCCTTGCCGGCACATGTGCATGTGCAGTGGTTGTACCAAGGTTCTGAGGTTCCAGACAAATAGATGCCCCGTCTGTAGGCAACCAGTTGAACGGCTCTTGGAAATCAAGGTGACCAATGGAGCTGATGGTGCTTTAGGTTAG

Protein Analysis

355

Amino Acids

39.31

Weight (kDa)

5.33

Isoelectric Point (pI)

41.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNF157-like_N PF26192 148 - 272 2.4e-49 RNF157-like N-terminal domain
zf-C3HC4_3 PF13920 293 - 339 4.4e-15 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012841)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G53410
fragaria_vesca FvH4_3g32531 FvH4_3g32531
malus_domestica MD03G1116400.v1.1 MD11G1134600.v1.1
prunus_persica Prupe.6G100500_v2.0.a1
pyrus_communis pycom11g11170
rosa_chinensis RchiOBHm_Chr5g0059601
rosa_laevigata RLG00000035307 RLG00000035309
rosa_multiflora Rmu_ssc0000488.1_g000001
rosa_roxburghii Rroxscaffold_1G00021010
rosa_rugosa Rorug05G0323600
rosa_samantha Rh5AG388600 Rh5BG400200 Rh5CG424500 Rh5DG416500
rosa_wichuraiana Rw5G036590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 374
AccII CGCG 1 cut(s) 353
AclWI GGATC 4 cut(s) 55, 68, 192, 587
AcoI YGGCCR 1 cut(s) 361
AcsI RAATTY 2 cut(s) 700, 888
AcuI CTGAAG 2 cut(s) 275, 678
AcyI GRCGYC 2 cut(s) 234, 291
AfaI GTAC 2 cut(s) 761, 958
AfiI CCNNNNNNNGG 4 cut(s) 167, 346, 370, 999
AflIII ACRYGT 2 cut(s) 351, 936
AgsI TTSAA 5 cut(s) 484, 591, 740, 848, 1013
AhlI ACTAGT 1 cut(s) 95
AjnI CCWGG 4 cut(s) 56, 63, 345, 363
AluBI AGCT 6 cut(s) 253, 402, 507, 814, 872, 1046
AluI AGCT 6 cut(s) 253, 402, 507, 814, 872, 1046
Alw21I GWGCWC 2 cut(s) 404, 924
Alw26I GTCTC 1 cut(s) 240
AlwI GGATC 4 cut(s) 55, 68, 192, 587
Ama87I CYCGRG 1 cut(s) 906
AoxI GGCC 4 cut(s) 26, 54, 361, 406
ApeKI GCWGC 2 cut(s) 368, 814
ApoI RAATTY 2 cut(s) 700, 888
AspS9I GGNCC 6 cut(s) 27, 54, 67, 87, 205, 407
AsuC2I CCSGG 3 cut(s) 209, 298, 709
AsuHPI GGTGA 8 cut(s) 296, 635, 661, 692, 767, 787, 863, 1045
AvaI CYCGRG 1 cut(s) 906
AvaII GGWCC 3 cut(s) 67, 87, 205
BaeI ACNNNNGTAYC 2 cut(s) 903, 936
BalI TGGCCA 1 cut(s) 363
BamHI GGATCC 1 cut(s) 60
BanI GGYRCC 1 cut(s) 374
BanII GRGCYC 1 cut(s) 404
BauI CACGAG 1 cut(s) 668
BbsI GAAGAC 1 cut(s) 323
Bbv12I GWGCWC 2 cut(s) 404, 924
BbvI GCAGC 2 cut(s) 380, 801
BccI CCATC 1 cut(s) 1043
BceAI ACGGC 4 cut(s) 126, 221, 514, 1031
BciT130I CCWGG 4 cut(s) 58, 65, 347, 365
BcnI CCSGG 3 cut(s) 209, 298, 709
BcoDI GTCTC 1 cut(s) 240
BcuI ACTAGT 1 cut(s) 95
BfaI CTAG 1 cut(s) 96
BfmI CTRYAG 3 cut(s) 439, 815, 997
BglII AGATCT 1 cut(s) 822
BisI GCNGC 7 cut(s) 221, 224, 294, 343, 369, 378, 815
BlsI GCNGC 7 cut(s) 222, 225, 295, 344, 370, 379, 816
Bme1390I CCNGG 7 cut(s) 58, 65, 209, 298, 347, 365, 709
Bme18I GGWCC 3 cut(s) 67, 87, 205
BmeT110I CYCGRG 1 cut(s) 906
BmgT120I GGNCC 6 cut(s) 27, 54, 67, 87, 205, 407
BmiI GGNNCC 6 cut(s) 62, 88, 207, 376, 409, 974
BmrFI CCNGG 7 cut(s) 58, 65, 209, 298, 347, 365, 709
BmrI ACTGGG 1 cut(s) 585
BmsI GCATC 3 cut(s) 310, 463, 977
BmuI ACTGGG 1 cut(s) 585
BoxI GACNNNNGTC 1 cut(s) 540
BpiI GAAGAC 1 cut(s) 323
BpuMI CCSGG 3 cut(s) 209, 298, 709
BsaHI GRCGYC 2 cut(s) 234, 291
BsaJI CCNNGG 3 cut(s) 180, 311, 960
BsaXI ACNNNNNCTCC 2 cut(s) 593, 623
Bsc4I CCNNNNNNNGG 4 cut(s) 167, 346, 370, 999
Bse118I RCCGGY 1 cut(s) 930
Bse1I ACTGG 2 cut(s) 580, 1007
BseBI CCWGG 4 cut(s) 58, 65, 347, 365
BseDI CCNNGG 3 cut(s) 180, 311, 960
BseLI CCNNNNNNNGG 4 cut(s) 167, 346, 370, 999
BseMII CTCAG 2 cut(s) 731, 959
BseNI ACTGG 2 cut(s) 580, 1007
BseRI GAGGAG 2 cut(s) 278, 614
BseXI GCAGC 2 cut(s) 380, 801
BseYI CCCAGC 1 cut(s) 216
BsgI GTGCAG 1 cut(s) 967
Bsh1236I CGCG 1 cut(s) 353
BshFI GGCC 4 cut(s) 28, 56, 363, 408
BshNI GGYRCC 1 cut(s) 374
BsiHKAI GWGCWC 2 cut(s) 404, 924
BsiHKCI CYCGRG 1 cut(s) 906
BsiSI CCGG 6 cut(s) 209, 287, 297, 405, 708, 931
BslI CCNNNNNNNGG 4 cut(s) 167, 346, 370, 999
BsmAI GTCTC 1 cut(s) 240
BsmI GAATGC 1 cut(s) 844
BsnI GGCC 4 cut(s) 28, 56, 363, 408
BsoBI CYCGRG 1 cut(s) 906
Bsp1286I GDGCHC 2 cut(s) 404, 924
Bsp143I GATC 6 cut(s) 18, 60, 184, 579, 720, 822
BspANI GGCC 4 cut(s) 28, 56, 363, 408
BspCNI CTCAG 2 cut(s) 730, 960
BspFNI CGCG 1 cut(s) 353
BspLI GGNNCC 6 cut(s) 62, 88, 207, 376, 409, 974
BspMAI CTGCAG 1 cut(s) 819
BspPI GGATC 4 cut(s) 55, 68, 192, 587
BspT107I GGYRCC 1 cut(s) 374
BsrFI RCCGGY 1 cut(s) 930
BsrI ACTGG 2 cut(s) 580, 1007
BssAI RCCGGY 1 cut(s) 930
BssECI CCNNGG 3 cut(s) 180, 311, 960
BssMI GATC 6 cut(s) 18, 60, 184, 579, 720, 822
BssNI GRCGYC 2 cut(s) 234, 291
BssSI CACGAG 1 cut(s) 668
BssT1I CCWWGG 1 cut(s) 960
Bst2BI CACGAG 1 cut(s) 668
Bst2UI CCWGG 4 cut(s) 58, 65, 347, 365
Bst4CI ACNGT 6 cut(s) 170, 391, 451, 623, 884, 919
BstACI GRCGYC 2 cut(s) 234, 291
BstAPI GCANNNNNTGC 1 cut(s) 374
BstC8I GCNNGC 2 cut(s) 509, 932
BstDEI CTNAG 2 cut(s) 717, 968
BstDSI CCRYGG 2 cut(s) 180, 311
BstEII GGTNACC 1 cut(s) 1033
BstFNI CGCG 1 cut(s) 353
BstKTI GATC 6 cut(s) 21, 63, 187, 582, 723, 825
BstMAI GTCTC 1 cut(s) 240
BstMBI GATC 6 cut(s) 18, 60, 184, 579, 720, 822
BstMWI GCNNNNNNNGC 4 cut(s) 199, 374, 377, 1052
BstNI CCWGG 4 cut(s) 58, 65, 347, 365
BstNSI RCATGY 2 cut(s) 940, 946
BstPAI GACNNNNGTC 1 cut(s) 540
BstPI GGTNACC 1 cut(s) 1033
BstSCI CCNGG 7 cut(s) 56, 63, 207, 296, 345, 363, 707
BstSFI CTRYAG 3 cut(s) 439, 815, 997
BstUI CGCG 1 cut(s) 353
BstV1I GCAGC 2 cut(s) 380, 801
BstV2I GAAGAC 1 cut(s) 323
BstX2I RGATCY 2 cut(s) 60, 822
BstYI RGATCY 2 cut(s) 60, 822
BsuRI GGCC 4 cut(s) 28, 56, 363, 408
BtgI CCRYGG 2 cut(s) 180, 311
BtsI GCAGTG 2 cut(s) 738, 955
BtsIMutI CAGTG 3 cut(s) 396, 738, 955
Cac8I GCNNGC 2 cut(s) 509, 932
Cfr10I RCCGGY 1 cut(s) 930
Cfr13I GGNCC 6 cut(s) 27, 54, 67, 87, 205, 407
CseI GACGC 4 cut(s) 242, 299, 342, 359
Csp6I GTAC 2 cut(s) 760, 957
CviAII CATG 4 cut(s) 71, 454, 937, 943
CviQI GTAC 2 cut(s) 760, 957
DdeI CTNAG 2 cut(s) 717, 968
DpnI GATC 6 cut(s) 20, 62, 186, 581, 722, 824
DpnII GATC 6 cut(s) 18, 60, 184, 579, 720, 822
EaeI YGGCCR 1 cut(s) 361
EciI GGCGGA 1 cut(s) 329
Ecl136II GAGCTC 1 cut(s) 402
Eco130I CCWWGG 1 cut(s) 960
Eco24I GRGCYC 1 cut(s) 404
Eco47I GGWCC 3 cut(s) 67, 87, 205
Eco53kI GAGCTC 1 cut(s) 402
Eco57I CTGAAG 2 cut(s) 275, 678
Eco88I CYCGRG 1 cut(s) 906
Eco91I GGTNACC 1 cut(s) 1033
EcoICRI GAGCTC 1 cut(s) 402
EcoO109I RGGNCCY 1 cut(s) 205
EcoO65I GGTNACC 1 cut(s) 1033
EcoRII CCWGG 4 cut(s) 56, 63, 345, 363
EcoT14I CCWWGG 1 cut(s) 960
EcoT38I GRGCYC 1 cut(s) 404
ErhI CCWWGG 1 cut(s) 960
FaeI CATG 4 cut(s) 74, 457, 940, 946
FalI AAGNNNNNCTT 2 cut(s) 491, 523
FatI CATG 4 cut(s) 70, 453, 936, 942
FauI CCCGC 4 cut(s) 207, 252, 364, 762
Fnu4HI GCNGC 7 cut(s) 221, 224, 294, 343, 369, 378, 815
FriOI GRGCYC 1 cut(s) 404
Fsp4HI GCNGC 7 cut(s) 221, 224, 294, 343, 369, 378, 815
FspBI CTAG 1 cut(s) 96
GluI GCNGC 7 cut(s) 221, 224, 294, 343, 369, 378, 815
GsaI CCCAGC 1 cut(s) 220
HaeIII GGCC 4 cut(s) 28, 56, 363, 408
HapII CCGG 6 cut(s) 209, 287, 297, 405, 708, 931
HgaI GACGC 4 cut(s) 242, 299, 342, 359
Hin1I GRCGYC 2 cut(s) 234, 291
Hin1II CATG 4 cut(s) 74, 457, 940, 946
HincII GTYRAC 2 cut(s) 9, 612
HindII GTYRAC 2 cut(s) 9, 612
HindIII AAGCTT 1 cut(s) 505
HinfI GANTC 2 cut(s) 176, 689
HpaII CCGG 6 cut(s) 209, 287, 297, 405, 708, 931
HphI GGTGA 8 cut(s) 296, 635, 661, 692, 767, 787, 863, 1045
Hpy166II GTNNAC 3 cut(s) 9, 612, 683
Hpy188I TCNGA 5 cut(s) 91, 694, 720, 901, 969
Hpy188III TCNNGA 6 cut(s) 173, 243, 570, 670, 908, 976
Hpy8I GTNNAC 3 cut(s) 9, 612, 683
Hpy99I CGWCG 1 cut(s) 236
HpyAV CCTTC 7 cut(s) 263, 343, 576, 646, 671, 739, 842
HpyCH4III ACNGT 6 cut(s) 170, 391, 451, 623, 884, 919
HpyCH4IV ACGT 1 cut(s) 685
HpyCH4V TGCA 5 cut(s) 476, 817, 842, 942, 948
HpyF10VI GCNNNNNNNGC 4 cut(s) 199, 374, 377, 1052
HpyF3I CTNAG 2 cut(s) 717, 968
HpySE526I ACGT 1 cut(s) 685
Hsp92I GRCGYC 2 cut(s) 234, 291
Hsp92II CATG 4 cut(s) 74, 457, 940, 946
KroI GCCGGC 1 cut(s) 930
KroNI GCCGGC 1 cut(s) 932
Kzo9I GATC 6 cut(s) 18, 60, 184, 579, 720, 822
LmnI GCTCC 5 cut(s) 335, 399, 407, 927, 1043
Lsp1109I GCAGC 2 cut(s) 380, 801
LweI GCATC 3 cut(s) 310, 463, 977
MaeI CTAG 1 cut(s) 96
MaeII ACGT 1 cut(s) 685
MaeIII GTNAC 4 cut(s) 133, 170, 623, 1033
MalI GATC 6 cut(s) 20, 62, 186, 581, 722, 824
MboI GATC 6 cut(s) 18, 60, 184, 579, 720, 822
MboII GAAGA 3 cut(s) 328, 328, 482
MflI RGATCY 2 cut(s) 60, 822
MhlI GDGCHC 2 cut(s) 404, 924
MlsI TGGCCA 1 cut(s) 363
MluCI AATT 2 cut(s) 700, 888
MluI ACGCGT 1 cut(s) 351
MluNI TGGCCA 1 cut(s) 363
Mox20I TGGCCA 1 cut(s) 363
MroNI GCCGGC 1 cut(s) 930
MscI TGGCCA 1 cut(s) 363
MseI TTAA 3 cut(s) 116, 513, 522
MslI CAYNNNNRTG 1 cut(s) 941
Msp20I TGGCCA 1 cut(s) 363
MspA1I CMGCKG 1 cut(s) 371
MspI CCGG 6 cut(s) 209, 287, 297, 405, 708, 931
MspR9I CCNGG 7 cut(s) 58, 65, 209, 298, 347, 365, 709
Mva1269I GAATGC 1 cut(s) 844
MvaI CCWGG 4 cut(s) 58, 65, 347, 365
MvnI CGCG 1 cut(s) 353
MwoI GCNNNNNNNGC 4 cut(s) 199, 374, 377, 1052
NaeI GCCGGC 1 cut(s) 932
NciI CCSGG 3 cut(s) 209, 298, 709
NdeII GATC 6 cut(s) 18, 60, 184, 579, 720, 822
NgoMIV GCCGGC 1 cut(s) 930
NlaIII CATG 4 cut(s) 74, 457, 940, 946
NlaIV GGNNCC 6 cut(s) 62, 88, 207, 376, 409, 974
NmuCI GTSAC 3 cut(s) 170, 623, 1033
NspI RCATGY 2 cut(s) 940, 946
PaeR7I CTCGAG 1 cut(s) 906
PciI ACATGT 1 cut(s) 936
PctI GAATGC 1 cut(s) 844
PdiI GCCGGC 1 cut(s) 932
PfeI GAWTC 2 cut(s) 176, 689
PkrI GCNGC 7 cut(s) 222, 225, 295, 344, 370, 379, 816
PpuMI RGGWCCY 1 cut(s) 205
PscI ACATGT 1 cut(s) 936
PshAI GACNNNNGTC 1 cut(s) 540
Psp124BI GAGCTC 1 cut(s) 404
Psp5II RGGWCCY 1 cut(s) 205
Psp6I CCWGG 4 cut(s) 56, 63, 345, 363
PspEI GGTNACC 1 cut(s) 1033
PspFI CCCAGC 1 cut(s) 216
PspGI CCWGG 4 cut(s) 56, 63, 345, 363
PspN4I GGNNCC 6 cut(s) 62, 88, 207, 376, 409, 974
PspPI GGNCC 6 cut(s) 27, 54, 67, 87, 205, 407
PspPPI RGGWCCY 1 cut(s) 205
PstI CTGCAG 1 cut(s) 819
PsuI RGATCY 2 cut(s) 60, 822
RsaI GTAC 2 cut(s) 761, 958
RsaNI GTAC 2 cut(s) 760, 957
RseI CAYNNNNRTG 1 cut(s) 941
SacI GAGCTC 1 cut(s) 404
SaqAI TTAA 3 cut(s) 116, 513, 522
SatI GCNGC 7 cut(s) 221, 224, 294, 343, 369, 378, 815
Sau3AI GATC 6 cut(s) 18, 60, 184, 579, 720, 822
Sau96I GGNCC 6 cut(s) 27, 54, 67, 87, 205, 407
ScrFI CCNGG 7 cut(s) 58, 65, 209, 298, 347, 365, 709
SduI GDGCHC 2 cut(s) 404, 924
SfaNI GCATC 3 cut(s) 310, 463, 977
SfcI CTRYAG 3 cut(s) 439, 815, 997
Sfr274I CTCGAG 1 cut(s) 906
SinI GGWCC 3 cut(s) 67, 87, 205
SlaI CTCGAG 1 cut(s) 906
SmiMI CAYNNNNRTG 1 cut(s) 941
SmlI CTYRAG 1 cut(s) 906
SmoI CTYRAG 1 cut(s) 906
SpeI ACTAGT 1 cut(s) 95
Sse9I AATT 2 cut(s) 700, 888
SspMI CTAG 1 cut(s) 96
SstI GAGCTC 1 cut(s) 404
StyD4I CCNGG 7 cut(s) 56, 63, 207, 296, 345, 363, 707
StyI CCWWGG 1 cut(s) 960
TaaI ACNGT 6 cut(s) 170, 391, 451, 623, 884, 919
TaiI ACGT 1 cut(s) 688
TaqI TCGA 2 cut(s) 582, 907
TasI AATT 2 cut(s) 700, 888
TatI WGTACW 1 cut(s) 759
TauI GCSGC 5 cut(s) 223, 226, 296, 345, 380
TfiI GAWTC 2 cut(s) 176, 689
Tru1I TTAA 3 cut(s) 116, 513, 522
Tru9I TTAA 3 cut(s) 116, 513, 522
TscAI CASTG 3 cut(s) 396, 738, 955
TseFI GTSAC 3 cut(s) 170, 623, 1033
TseI GCWGC 2 cut(s) 368, 814
Tsp45I GTSAC 3 cut(s) 170, 623, 1033
TspDTI ATGAA 3 cut(s) 690, 870, 882
TspGWI ACGGA 2 cut(s) 197, 300
TspRI CASTG 3 cut(s) 396, 738, 955
VpaK11BI GGWCC 3 cut(s) 67, 87, 205
XapI RAATTY 2 cut(s) 700, 888
XceI RCATGY 2 cut(s) 940, 946
XhoI CTCGAG 1 cut(s) 906
XspI CTAG 1 cut(s) 96
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.