Rh5DG416500

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0012841
Physical Location & Seq
Reverse (-)
66216289 .. 66220264
3976 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG416500.1

Sequence Viewer

Length: 1035 bp
ATGGGAATCGGAAGCAGTAGCGCCGGTCGCCGGAGGCACCGGCCGCCTCCGCCTCCGGCTCATCCACAACCTCAAAATGGTGGCAGATATGGTTACGCTGGTGCCTCACCACCTTACCCGCCCCAATATCCAAACGAACCAATCACCAATCCAGGCCAGTACCACCAATACCCACATCAATTTCCCAACGGACTAAATCCCAATCAACCCCATTTCTACCAGTACTCTGCATTCTATCCACCGCCGCCGATACCGGGCGGGTACCACCGCGGCGGGGGTTTCATGGGCCCTCCTCCGCCGGGGAATTGGGTTCCGGGGCAGTACCCTTATGGTGCTCCTCCACCCCTTCCGGGTGCTCCCTTTGTTGAGCATCAGAAGACGGTTACTATCAAGAATGATGTGAATATCAAGAAGGACTCTTTGAGGGTTGAGCCGGATGAGGAGAATCCCGGCAAGTTTGTTGTTGCCTTCACTTTTGATGCCGCCGCACCCGGAAGCATTACCGTCATGTTTTTTACGAAAGAAGATGCCGACTTTAACTTGATAGCAACAAAGGAAAGCTTGCTTAAACCTGTTAAAGTGCCGTTTGACAAAGGTCTTGGTCAAAAGTTTAGACAACCTTCTGGAACTGGGATCGACTTTTCAATGTTTGAGGAGATAGGGTTGACAAAAGAGGGTGACATAGAAGTTTATCCTCTTGTGGTGAAAGCTGAAGCACATCTCGTGAATGAAGGTGAACGTGAATCAGAGGGAAATTCCACCGGGAACTCTCAGATCACACAGGCAGTGTTTGAAAAGAAGGAGAATGGTGAGTACAAAGTGCGGGTAGTGAAACAGATACTATGGGTGGATAATATGAGGTATGAGCTGCAGGAGATCTATGGTATTGGGAATGCAGTTGAAGGTGATCAAAATGATAATGAAGCTGGAAAAGACTGTATAATTTGCTTGTCAGAACCTCGAGATACAACTGTGCTCCCTTGCCGGCACATGGTACGTGGGGAATTTACTTCTTCCCTTCTCTTCTATTTGTAG

Protein Analysis

344

Amino Acids

38.06

Weight (kDa)

5.76

Isoelectric Point (pI)

44.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNF157-like_N PF26192 134 - 290 3e-67 RNF157-like N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012841)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G53410
fragaria_vesca FvH4_3g32531 FvH4_3g32531
malus_domestica MD03G1116400.v1.1 MD11G1134600.v1.1
prunus_persica Prupe.6G100500_v2.0.a1
pyrus_communis pycom11g11170
rosa_chinensis RchiOBHm_Chr5g0059601
rosa_laevigata RLG00000035307 RLG00000035309
rosa_multiflora Rmu_ssc0000488.1_g000001
rosa_roxburghii Rroxscaffold_1G00021010
rosa_rugosa Rorug05G0323600
rosa_samantha Rh5AG388600 Rh5BG400200 Rh5CG424500 Rh5DG416500
rosa_wichuraiana Rw5G036590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 261
AccB1I GGYRCC 3 cut(s) 36, 101, 261
AccII CGCG 1 cut(s) 270
AclWI GGATC 1 cut(s) 641
AcoI YGGCCR 1 cut(s) 41
AcsI RAATTY 2 cut(s) 754, 1004
AcuI CTGAAG 1 cut(s) 732
AfaI GTAC 6 cut(s) 161, 224, 263, 323, 815, 996
AfiI CCNNNNNNNGG 7 cut(s) 30, 77, 254, 274, 299, 350, 991
AgsI TTSAA 3 cut(s) 645, 794, 902
AjnI CCWGG 1 cut(s) 151
AluBI AGCT 4 cut(s) 561, 710, 868, 926
AluI AGCT 4 cut(s) 561, 710, 868, 926
Alw21I GWGCWC 3 cut(s) 337, 358, 978
AlwI GGATC 1 cut(s) 641
Ama87I CYCGRG 1 cut(s) 960
AoxI GGCC 3 cut(s) 41, 154, 286
ApaI GGGCCC 1 cut(s) 290
ApeKI GCWGC 1 cut(s) 868
ApoI RAATTY 2 cut(s) 754, 1004
Asp718I GGTACC 1 cut(s) 261
AspLEI GCGC 1 cut(s) 23
AspS9I GGNCC 2 cut(s) 286, 287
AsuC2I CCSGG 7 cut(s) 255, 300, 315, 351, 450, 492, 763
AsuHPI GGTGA 7 cut(s) 99, 136, 689, 715, 746, 821, 917
AvaI CYCGRG 1 cut(s) 960
BaeGI GKGCMC 1 cut(s) 290
BaeI ACNNNNGTAYC 2 cut(s) 957, 990
BanI GGYRCC 3 cut(s) 36, 101, 261
BanII GRGCYC 1 cut(s) 290
BauI CACGAG 1 cut(s) 722
BbsI GAAGAC 1 cut(s) 383
Bbv12I GWGCWC 3 cut(s) 337, 358, 978
BbvI GCAGC 1 cut(s) 855
BceAI ACGGC 1 cut(s) 568
BciT130I CCWGG 1 cut(s) 153
BclI TGATCA 1 cut(s) 907
BcnI CCSGG 7 cut(s) 255, 300, 315, 351, 450, 492, 763
BfmI CTRYAG 1 cut(s) 869
BfoI RGCGCY 1 cut(s) 24
BglII AGATCT 1 cut(s) 876
BisI GCNGC 6 cut(s) 44, 245, 271, 483, 486, 869
BlsI GCNGC 6 cut(s) 45, 246, 272, 484, 487, 870
BmcAI AGTACT 1 cut(s) 224
Bme1390I CCNGG 8 cut(s) 153, 255, 300, 315, 351, 450, 492, 763
BmeT110I CYCGRG 1 cut(s) 960
BmgT120I GGNCC 2 cut(s) 286, 287
BmiI GGNNCC 5 cut(s) 38, 103, 263, 288, 312
BmrFI CCNGG 8 cut(s) 153, 255, 300, 315, 351, 450, 492, 763
BmrI ACTGGG 1 cut(s) 639
BmsI GCATC 3 cut(s) 379, 469, 517
BmuI ACTGGG 1 cut(s) 639
BoxI GACNNNNGTC 1 cut(s) 594
BpiI GAAGAC 1 cut(s) 383
BpuMI CCSGG 7 cut(s) 255, 300, 315, 351, 450, 492, 763
BsaAI YACGTR 1 cut(s) 998
BsaJI CCNNGG 3 cut(s) 268, 299, 314
BsaXI ACNNNNNCTCC 2 cut(s) 647, 677
Bsc4I CCNNNNNNNGG 7 cut(s) 30, 77, 254, 274, 299, 350, 991
Bse118I RCCGGY 3 cut(s) 23, 39, 984
Bse1I ACTGG 3 cut(s) 157, 220, 634
BseBI CCWGG 1 cut(s) 153
BseDI CCNNGG 3 cut(s) 268, 299, 314
BseGI GGATG 2 cut(s) 61, 442
BseLI CCNNNNNNNGG 7 cut(s) 30, 77, 254, 274, 299, 350, 991
BseMII CTCAG 1 cut(s) 785
BseNI ACTGG 3 cut(s) 157, 220, 634
BseRI GAGGAG 4 cut(s) 282, 327, 455, 668
BseSI GKGCMC 1 cut(s) 290
BseX3I CGGCCG 1 cut(s) 41
BseXI GCAGC 1 cut(s) 855
Bsh1236I CGCG 1 cut(s) 270
Bsh1285I CGRYCG 2 cut(s) 28, 44
BshFI GGCC 3 cut(s) 43, 156, 288
BshNI GGYRCC 3 cut(s) 36, 101, 261
BsiEI CGRYCG 2 cut(s) 28, 44
BsiHKAI GWGCWC 3 cut(s) 337, 358, 978
BsiHKCI CYCGRG 1 cut(s) 960
BslI CCNNNNNNNGG 7 cut(s) 30, 77, 254, 274, 299, 350, 991
BsmI GAATGC 2 cut(s) 230, 898
BsnI GGCC 3 cut(s) 43, 156, 288
BsoBI CYCGRG 1 cut(s) 960
Bsp120I GGGCCC 1 cut(s) 286
Bsp1286I GDGCHC 4 cut(s) 290, 337, 358, 978
Bsp143I GATC 4 cut(s) 633, 774, 876, 907
BspANI GGCC 3 cut(s) 43, 156, 288
BspCNI CTCAG 1 cut(s) 784
BspFNI CGCG 1 cut(s) 270
BspLI GGNNCC 5 cut(s) 38, 103, 263, 288, 312
BspMAI CTGCAG 1 cut(s) 873
BspPI GGATC 1 cut(s) 641
BspT107I GGYRCC 3 cut(s) 36, 101, 261
BsrFI RCCGGY 3 cut(s) 23, 39, 984
BsrI ACTGG 3 cut(s) 157, 220, 634
BssAI RCCGGY 3 cut(s) 23, 39, 984
BssECI CCNNGG 3 cut(s) 268, 299, 314
BssMI GATC 4 cut(s) 633, 774, 876, 907
BssSI CACGAG 1 cut(s) 722
Bst2BI CACGAG 1 cut(s) 722
Bst2UI CCWGG 1 cut(s) 153
Bst4CI ACNGT 4 cut(s) 382, 505, 938, 973
Bst6I CTCTTC 1 cut(s) 1028
BstBAI YACGTR 1 cut(s) 998
BstC8I GCNNGC 2 cut(s) 563, 986
BstDEI CTNAG 1 cut(s) 771
BstDSI CCRYGG 1 cut(s) 268
BstF5I GGATG 2 cut(s) 61, 442
BstFNI CGCG 1 cut(s) 270
BstH2I RGCGCY 1 cut(s) 24
BstHHI GCGC 1 cut(s) 23
BstKTI GATC 4 cut(s) 636, 777, 879, 910
BstMBI GATC 4 cut(s) 633, 774, 876, 907
BstMCI CGRYCG 2 cut(s) 28, 44
BstMWI GCNNNNNNNGC 3 cut(s) 27, 43, 49
BstNI CCWGG 1 cut(s) 153
BstPAI GACNNNNGTC 1 cut(s) 594
BstSCI CCNGG 8 cut(s) 151, 253, 298, 313, 349, 448, 490, 761
BstSFI CTRYAG 1 cut(s) 869
BstSLI GKGCMC 1 cut(s) 290
BstUI CGCG 1 cut(s) 270
BstV1I GCAGC 1 cut(s) 855
BstV2I GAAGAC 1 cut(s) 383
BstX2I RGATCY 1 cut(s) 876
BstYI RGATCY 1 cut(s) 876
BstZI CGGCCG 1 cut(s) 41
BsuRI GGCC 3 cut(s) 43, 156, 288
BtgI CCRYGG 1 cut(s) 268
BtsCI GGATG 2 cut(s) 61, 442
BtsI GCAGTG 1 cut(s) 792
BtsIMutI CAGTG 1 cut(s) 792
Cac8I GCNNGC 2 cut(s) 563, 986
CfoI GCGC 1 cut(s) 23
Cfr10I RCCGGY 3 cut(s) 23, 39, 984
Cfr13I GGNCC 2 cut(s) 286, 287
Cfr42I CCGCGG 1 cut(s) 271
Csp6I GTAC 6 cut(s) 160, 223, 262, 322, 814, 995
CviAII CATG 3 cut(s) 283, 508, 991
CviJI RGCY 9 cut(s) 43, 59, 156, 288, 433, 561, 710, 868, 926
CviKI_1 RGCY 9 cut(s) 43, 59, 156, 288, 433, 561, 710, 868, 926
CviQI GTAC 6 cut(s) 160, 223, 262, 322, 814, 995
DdeI CTNAG 1 cut(s) 771
DpnI GATC 4 cut(s) 635, 776, 878, 909
DpnII GATC 4 cut(s) 633, 774, 876, 907
EaeI YGGCCR 1 cut(s) 41
EagI CGGCCG 1 cut(s) 41
Eam1104I CTCTTC 1 cut(s) 1028
EarI CTCTTC 1 cut(s) 1028
EciI GGCGGA 2 cut(s) 39, 285
EclXI CGGCCG 1 cut(s) 41
Eco24I GRGCYC 1 cut(s) 290
Eco52I CGGCCG 1 cut(s) 41
Eco57I CTGAAG 1 cut(s) 732
Eco88I CYCGRG 1 cut(s) 960
EcoO109I RGGNCCY 1 cut(s) 287
EcoRII CCWGG 1 cut(s) 151
EcoT38I GRGCYC 1 cut(s) 290
FaeI CATG 3 cut(s) 286, 511, 994
FalI AAGNNNNNCTT 2 cut(s) 545, 577
FatI CATG 3 cut(s) 282, 507, 990
FauI CCCGC 4 cut(s) 126, 251, 266, 816
FbaI TGATCA 1 cut(s) 907
Fnu4HI GCNGC 6 cut(s) 44, 245, 271, 483, 486, 869
FokI GGATG 2 cut(s) 48, 449
FriOI GRGCYC 1 cut(s) 290
Fsp4HI GCNGC 6 cut(s) 44, 245, 271, 483, 486, 869
GlaI GCGC 1 cut(s) 22
GluI GCNGC 6 cut(s) 44, 245, 271, 483, 486, 869
HaeII RGCGCY 1 cut(s) 24
HaeIII GGCC 3 cut(s) 43, 156, 288
HhaI GCGC 1 cut(s) 23
Hin1II CATG 3 cut(s) 286, 511, 994
Hin6I GCGC 1 cut(s) 21
HinP1I GCGC 1 cut(s) 21
HincII GTYRAC 1 cut(s) 666
HindII GTYRAC 1 cut(s) 666
HindIII AAGCTT 1 cut(s) 559
HinfI GANTC 4 cut(s) 6, 416, 445, 743
HphI GGTGA 7 cut(s) 99, 136, 689, 715, 746, 821, 917
Hpy166II GTNNAC 2 cut(s) 666, 737
Hpy188I TCNGA 5 cut(s) 11, 375, 748, 774, 955
Hpy188III TCNNGA 5 cut(s) 391, 409, 624, 724, 962
Hpy8I GTNNAC 2 cut(s) 666, 737
HpyAV CCTTC 8 cut(s) 356, 406, 478, 630, 725, 793, 896, 1028
HpyCH4III ACNGT 4 cut(s) 382, 505, 938, 973
HpyCH4IV ACGT 2 cut(s) 739, 997
HpyCH4V TGCA 3 cut(s) 230, 871, 896
HpyF10VI GCNNNNNNNGC 3 cut(s) 27, 43, 49
HpyF3I CTNAG 1 cut(s) 771
HpySE526I ACGT 2 cut(s) 739, 997
Hsp92II CATG 3 cut(s) 286, 511, 994
HspAI GCGC 1 cut(s) 21
KpnI GGTACC 1 cut(s) 265
KroI GCCGGC 1 cut(s) 984
KroNI GCCGGC 1 cut(s) 986
Ksp22I TGATCA 1 cut(s) 907
KspI CCGCGG 1 cut(s) 271
Kzo9I GATC 4 cut(s) 633, 774, 876, 907
LmnI GCTCC 3 cut(s) 340, 361, 981
Lsp1109I GCAGC 1 cut(s) 855
LweI GCATC 3 cut(s) 379, 469, 517
MaeII ACGT 2 cut(s) 739, 997
MaeIII GTNAC 3 cut(s) 92, 382, 677
MalI GATC 4 cut(s) 635, 776, 878, 909
MboI GATC 4 cut(s) 633, 774, 876, 907
MboII GAAGA 4 cut(s) 388, 536, 1005, 1015
MflI RGATCY 1 cut(s) 876
MhlI GDGCHC 4 cut(s) 290, 337, 358, 978
MluCI AATT 5 cut(s) 179, 304, 754, 942, 1004
MlyI GAGTC 1 cut(s) 410
MroNI GCCGGC 1 cut(s) 984
MseI TTAA 3 cut(s) 537, 567, 576
MspA1I CMGCKG 1 cut(s) 270
MspR9I CCNGG 8 cut(s) 153, 255, 300, 315, 351, 450, 492, 763
Mva1269I GAATGC 2 cut(s) 230, 898
MvaI CCWGG 1 cut(s) 153
MvnI CGCG 1 cut(s) 270
MwoI GCNNNNNNNGC 3 cut(s) 27, 43, 49
NaeI GCCGGC 1 cut(s) 986
NciI CCSGG 7 cut(s) 255, 300, 315, 351, 450, 492, 763
NdeII GATC 4 cut(s) 633, 774, 876, 907
NgoMIV GCCGGC 1 cut(s) 984
NlaIII CATG 3 cut(s) 286, 511, 994
NlaIV GGNNCC 5 cut(s) 38, 103, 263, 288, 312
NmuCI GTSAC 1 cut(s) 677
PaeR7I CTCGAG 1 cut(s) 960
PctI GAATGC 2 cut(s) 230, 898
PdiI GCCGGC 1 cut(s) 986
PfeI GAWTC 3 cut(s) 6, 445, 743
PkrI GCNGC 6 cut(s) 45, 246, 272, 484, 487, 870
PleI GAGTC 1 cut(s) 410
PpsI GAGTC 1 cut(s) 410
Ppu21I YACGTR 1 cut(s) 998
PshAI GACNNNNGTC 1 cut(s) 594
Psp6I CCWGG 1 cut(s) 151
PspGI CCWGG 1 cut(s) 151
PspN4I GGNNCC 5 cut(s) 38, 103, 263, 288, 312
PspOMI GGGCCC 1 cut(s) 286
PspPI GGNCC 2 cut(s) 286, 287
PstI CTGCAG 1 cut(s) 873
PsuI RGATCY 1 cut(s) 876
RsaI GTAC 6 cut(s) 161, 224, 263, 323, 815, 996
RsaNI GTAC 6 cut(s) 160, 223, 262, 322, 814, 995
SacII CCGCGG 1 cut(s) 271
SaqAI TTAA 3 cut(s) 537, 567, 576
SatI GCNGC 6 cut(s) 44, 245, 271, 483, 486, 869
Sau3AI GATC 4 cut(s) 633, 774, 876, 907
Sau96I GGNCC 2 cut(s) 286, 287
ScaI AGTACT 1 cut(s) 224
SchI GAGTC 1 cut(s) 410
ScrFI CCNGG 8 cut(s) 153, 255, 300, 315, 351, 450, 492, 763
SduI GDGCHC 4 cut(s) 290, 337, 358, 978
SfaNI GCATC 3 cut(s) 379, 469, 517
SfcI CTRYAG 1 cut(s) 869
Sfr274I CTCGAG 1 cut(s) 960
Sfr303I CCGCGG 1 cut(s) 271
SgrBI CCGCGG 1 cut(s) 271
SlaI CTCGAG 1 cut(s) 960
SmlI CTYRAG 1 cut(s) 960
SmoI CTYRAG 1 cut(s) 960
Sse9I AATT 5 cut(s) 179, 304, 754, 942, 1004
StyD4I CCNGG 8 cut(s) 151, 253, 298, 313, 349, 448, 490, 761
TaaI ACNGT 4 cut(s) 382, 505, 938, 973
TaiI ACGT 2 cut(s) 742, 1000
TaqI TCGA 2 cut(s) 636, 961
TasI AATT 5 cut(s) 179, 304, 754, 942, 1004
TatI WGTACW 2 cut(s) 222, 813
TauI GCSGC 5 cut(s) 46, 247, 273, 485, 488
TfiI GAWTC 3 cut(s) 6, 445, 743
Tru1I TTAA 3 cut(s) 537, 567, 576
Tru9I TTAA 3 cut(s) 537, 567, 576
TscAI CASTG 1 cut(s) 792
TseFI GTSAC 1 cut(s) 677
TseI GCWGC 1 cut(s) 868
Tsp45I GTSAC 1 cut(s) 677
TspDTI ATGAA 3 cut(s) 271, 744, 936
TspGWI ACGGA 1 cut(s) 204
TspRI CASTG 1 cut(s) 792
XapI RAATTY 2 cut(s) 754, 1004
XhoI CTCGAG 1 cut(s) 960
ZrmI AGTACT 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.