Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0012841

Physical Location & Seq

Genomic Coordinates Forward (+)

25646027 .. 25648779

Gene Structure 2753 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00021010.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1119 bp

ATGGGAATCGGAAGCAGTAGCGCCGGTCGCCGGAGGCACCGGCCGCCTCCGCCTCCGGCCCATCCACAACCTCAAAATGGTGGCAGATATGGTTACGCTGGTGCCTCACCACCTTACCCGCCCCAATATCCAAACGAACCAATCCCCAATCCAGGCCAGTACCACCAATACCCACATCAATTTCCCAACGGACCAAATCCCAATCAAGCCCATTTCTACCAGTACTCTGCATTCTATCCACCGCCGCCGATACCGGCCGGGTACCACCGCGGCGGGGCTTTCATGGGCCCTCCTCCGCCGGGCAATTGGGTTCCGGGGCAGTACCCTTATGGTGCTCCTCCACCCCTTCCGGGTGCTCCCTTTGTTGAGCATCAGAAGACGGTTACTATCAAGAATGATGTGAATATCAAGAAGGACTCTTTGAGGGTTGAGCCGGATGAGGAGAATCCCGGCAAGTTTGTTGTTGCCTTCACTTTTGATGCTGCCGCACCCGGAAGCATTACCGTCATGTTTTTTACGAAAGAAGATGCAGACTTTAACTTGATAGCAACAAAGGAAAGCTTGCTTAGACCTGTTAAAGTGCCGTTTGACAAAGGTCTTGGTCAAAAGTTTAGACAACCTTCTGGAACTGGGATCGACTTTTCAATGTTTGAGGAGATAGGGTTGACAAAAGAGGGTGACATAGAAGTTTATCCTCTTGTGGTGAAGGCTGAAGCACATCTCGTGAATGAAGGTGAACGTGAATCAGAGGGAAATTCCACCGGGAACTCTCAGATCACACAGGCAGTGTTTGAAAAGAAGGAGAATGGTGAGTACAAAGTGCGGGTAGTGAAACAGATACTATGGGTGGATAATATGAGGTATGAGCTGCAGGAGATCTATGGTATTGGGAATGCAGTTGAAGGTGATCAAAATGATAATGAAGCTGGAAAAGACTGTATAATTTGCTTGTCAGAACCTCGAGATACAACTGTGCTCCCTTGCCGGCACATGTGCATGTGCAGTGGTTGTGCCAAGGTTCTGAGGTTCCAGACAAATAGATGCCCCGTCTGTAGGCAACCAGTTGAACGGCTCTTGGAAATCAAGGTGACCAATGGAGCTGATGGTGCTTTAGGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0008277 GO:0008589 GO:0009966 GO:0009968 GO:0009987 GO:0010646 GO:0010648 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0023051 GO:0023057 GO:0032446 GO:0036211 GO:0043170 GO:0043412 GO:0043949 GO:0043951 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0045744 GO:0045879 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0065007 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1902531 GO:1902532

KEGG Pathways

Metabolic & Signaling

ko04120 map04120

Pfam Domains

Protein Families

zf-C3HC4_3

Protein Analysis

372

Amino Acids

40.97

Weight (kDa)

6.17

Isoelectric Point (pI)

42.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNF157-like_N	PF26192	134 - 290	5.7e-67	RNF157-like N-terminal domain
zf-C3HC4_3	PF13920	311 - 357	9.2e-16	Zinc finger, C3HC4 type (RING finger)
zf-RING_2	PF13639	312 - 352	2.6e-06	Ring finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0012841)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G53410
fragaria_vesca	FvH4_3g32531 FvH4_3g32531
malus_domestica	MD03G1116400.v1.1 MD11G1134600.v1.1
prunus_persica	Prupe.6G100500_v2.0.a1
pyrus_communis	pycom11g11170
rosa_chinensis	RchiOBHm_Chr5g0059601
rosa_laevigata	RLG00000035307 RLG00000035309
rosa_multiflora	Rmu_ssc0000488.1_g000001
rosa_roxburghii	Rroxscaffold_1G00021010
rosa_rugosa	Rorug05G0323600
rosa_samantha	Rh5AG388600 Rh5BG400200 Rh5CG424500 Rh5DG416500
rosa_wichuraiana	Rw5G036590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	261
AccB1I	GGYRCC	3 cut(s)	36, 101, 261
AccII	CGCG	1 cut(s)	270
AclWI	GGATC	1 cut(s)	641
AcoI	YGGCCR	2 cut(s)	41, 255
AcsI	RAATTY	1 cut(s)	754
AcuI	CTGAAG	1 cut(s)	732
AfaI	GTAC	5 cut(s)	161, 224, 263, 323, 815
AfiI	CCNNNNNNNGG	7 cut(s)	30, 77, 152, 274, 299, 350, 1053
AflIII	ACRYGT	1 cut(s)	990
AgsI	TTSAA	4 cut(s)	645, 794, 902, 1067
AjnI	CCWGG	1 cut(s)	151
AluBI	AGCT	4 cut(s)	561, 868, 926, 1100
AluI	AGCT	4 cut(s)	561, 868, 926, 1100
Alw21I	GWGCWC	3 cut(s)	337, 358, 978
AlwI	GGATC	1 cut(s)	641
Ama87I	CYCGRG	1 cut(s)	960
AoxI	GGCC	5 cut(s)	41, 57, 154, 255, 286
ApaI	GGGCCC	1 cut(s)	290
ApeKI	GCWGC	2 cut(s)	482, 868
ApoI	RAATTY	1 cut(s)	754
Asp718I	GGTACC	1 cut(s)	261
AspLEI	GCGC	1 cut(s)	23
AspS9I	GGNCC	4 cut(s)	58, 191, 286, 287
AsuC2I	CCSGG	7 cut(s)	259, 300, 315, 351, 450, 492, 763
AsuHPI	GGTGA	7 cut(s)	99, 689, 715, 746, 821, 917, 1099
AvaI	CYCGRG	1 cut(s)	960
AvaII	GGWCC	1 cut(s)	191
BaeGI	GKGCMC	1 cut(s)	290
BaeI	ACNNNNGTAYC	2 cut(s)	957, 990
BanI	GGYRCC	3 cut(s)	36, 101, 261
BanII	GRGCYC	1 cut(s)	290
BauI	CACGAG	1 cut(s)	722
BbsI	GAAGAC	1 cut(s)	383
Bbv12I	GWGCWC	3 cut(s)	337, 358, 978
BbvI	GCAGC	2 cut(s)	469, 855
BccI	CCATC	2 cut(s)	69, 1097
BceAI	ACGGC	2 cut(s)	568, 1085
BciT130I	CCWGG	1 cut(s)	153
BclI	TGATCA	1 cut(s)	907
BcnI	CCSGG	7 cut(s)	259, 300, 315, 351, 450, 492, 763
BfmI	CTRYAG	2 cut(s)	869, 1051
BfoI	RGCGCY	1 cut(s)	24
BglII	AGATCT	1 cut(s)	876
BisI	GCNGC	6 cut(s)	44, 245, 271, 483, 486, 869
BlsI	GCNGC	6 cut(s)	45, 246, 272, 484, 487, 870
BmcAI	AGTACT	1 cut(s)	224
Bme1390I	CCNGG	8 cut(s)	153, 259, 300, 315, 351, 450, 492, 763
Bme18I	GGWCC	1 cut(s)	191
BmeT110I	CYCGRG	1 cut(s)	960
BmgT120I	GGNCC	4 cut(s)	58, 191, 286, 287
BmiI	GGNNCC	6 cut(s)	38, 103, 263, 288, 312, 1028
BmrFI	CCNGG	8 cut(s)	153, 259, 300, 315, 351, 450, 492, 763
BmrI	ACTGGG	1 cut(s)	639
BmsI	GCATC	4 cut(s)	379, 469, 517, 1031
BmuI	ACTGGG	1 cut(s)	639
BoxI	GACNNNNGTC	1 cut(s)	594
BpiI	GAAGAC	1 cut(s)	383
BpuMI	CCSGG	7 cut(s)	259, 300, 315, 351, 450, 492, 763
BsaJI	CCNNGG	3 cut(s)	268, 314, 1014
BsaXI	ACNNNNNCTCC	2 cut(s)	647, 677
Bsc4I	CCNNNNNNNGG	7 cut(s)	30, 77, 152, 274, 299, 350, 1053
Bse118I	RCCGGY	4 cut(s)	23, 39, 253, 984
Bse1I	ACTGG	4 cut(s)	157, 220, 634, 1061
BseBI	CCWGG	1 cut(s)	153
BseDI	CCNNGG	3 cut(s)	268, 314, 1014
BseGI	GGATG	2 cut(s)	61, 442
BseLI	CCNNNNNNNGG	7 cut(s)	30, 77, 152, 274, 299, 350, 1053
BseMII	CTCAG	2 cut(s)	785, 1013
BseNI	ACTGG	4 cut(s)	157, 220, 634, 1061
BseRI	GAGGAG	4 cut(s)	282, 327, 455, 668
BseSI	GKGCMC	1 cut(s)	290
BseX3I	CGGCCG	2 cut(s)	41, 255
BseXI	GCAGC	2 cut(s)	469, 855
BsgI	GTGCAG	1 cut(s)	1021
Bsh1236I	CGCG	1 cut(s)	270
Bsh1285I	CGRYCG	3 cut(s)	28, 44, 258
BshFI	GGCC	5 cut(s)	43, 59, 156, 257, 288
BshNI	GGYRCC	3 cut(s)	36, 101, 261
BsiEI	CGRYCG	3 cut(s)	28, 44, 258
BsiHKAI	GWGCWC	3 cut(s)	337, 358, 978
BsiHKCI	CYCGRG	1 cut(s)	960
BslI	CCNNNNNNNGG	7 cut(s)	30, 77, 152, 274, 299, 350, 1053
BsmI	GAATGC	2 cut(s)	230, 898
BsnI	GGCC	5 cut(s)	43, 59, 156, 257, 288
BsoBI	CYCGRG	1 cut(s)	960
Bsp120I	GGGCCC	1 cut(s)	286
Bsp1286I	GDGCHC	4 cut(s)	290, 337, 358, 978
Bsp143I	GATC	4 cut(s)	633, 774, 876, 907
BspANI	GGCC	5 cut(s)	43, 59, 156, 257, 288
BspCNI	CTCAG	2 cut(s)	784, 1014
BspFNI	CGCG	1 cut(s)	270
BspLI	GGNNCC	6 cut(s)	38, 103, 263, 288, 312, 1028
BspMAI	CTGCAG	1 cut(s)	873
BspPI	GGATC	1 cut(s)	641
BspT107I	GGYRCC	3 cut(s)	36, 101, 261
BsrFI	RCCGGY	4 cut(s)	23, 39, 253, 984
BsrI	ACTGG	4 cut(s)	157, 220, 634, 1061
BssAI	RCCGGY	4 cut(s)	23, 39, 253, 984
BssECI	CCNNGG	3 cut(s)	268, 314, 1014
BssMI	GATC	4 cut(s)	633, 774, 876, 907
BssSI	CACGAG	1 cut(s)	722
BssT1I	CCWWGG	1 cut(s)	1014
Bst2BI	CACGAG	1 cut(s)	722
Bst2UI	CCWGG	1 cut(s)	153
Bst4CI	ACNGT	4 cut(s)	382, 505, 938, 973
BstC8I	GCNNGC	2 cut(s)	563, 986
BstDEI	CTNAG	3 cut(s)	566, 771, 1022
BstDSI	CCRYGG	1 cut(s)	268
BstEII	GGTNACC	1 cut(s)	1087
BstF5I	GGATG	2 cut(s)	61, 442
BstFNI	CGCG	1 cut(s)	270
BstH2I	RGCGCY	1 cut(s)	24
BstHHI	GCGC	1 cut(s)	23
BstKTI	GATC	4 cut(s)	636, 777, 879, 910
BstMBI	GATC	4 cut(s)	633, 774, 876, 907
BstMCI	CGRYCG	3 cut(s)	28, 44, 258
BstMWI	GCNNNNNNNGC	4 cut(s)	27, 43, 49, 1106
BstNI	CCWGG	1 cut(s)	153
BstNSI	RCATGY	2 cut(s)	994, 1000
BstPAI	GACNNNNGTC	1 cut(s)	594
BstPI	GGTNACC	1 cut(s)	1087
BstSCI	CCNGG	8 cut(s)	151, 257, 298, 313, 349, 448, 490, 761
BstSFI	CTRYAG	2 cut(s)	869, 1051
BstSLI	GKGCMC	1 cut(s)	290
BstUI	CGCG	1 cut(s)	270
BstV1I	GCAGC	2 cut(s)	469, 855
BstV2I	GAAGAC	1 cut(s)	383
BstX2I	RGATCY	1 cut(s)	876
BstYI	RGATCY	1 cut(s)	876
BstZI	CGGCCG	2 cut(s)	41, 255
BsuRI	GGCC	5 cut(s)	43, 59, 156, 257, 288
BtgI	CCRYGG	1 cut(s)	268
BtsCI	GGATG	2 cut(s)	61, 442
BtsI	GCAGTG	2 cut(s)	792, 1009
BtsIMutI	CAGTG	2 cut(s)	792, 1009
Cac8I	GCNNGC	2 cut(s)	563, 986
CfoI	GCGC	1 cut(s)	23
Cfr10I	RCCGGY	4 cut(s)	23, 39, 253, 984
Cfr13I	GGNCC	4 cut(s)	58, 191, 286, 287
Cfr42I	CCGCGG	1 cut(s)	271
Csp6I	GTAC	5 cut(s)	160, 223, 262, 322, 814
CviAII	CATG	4 cut(s)	283, 508, 991, 997
CviQI	GTAC	5 cut(s)	160, 223, 262, 322, 814
DdeI	CTNAG	3 cut(s)	566, 771, 1022
DpnI	GATC	4 cut(s)	635, 776, 878, 909
DpnII	GATC	4 cut(s)	633, 774, 876, 907
EaeI	YGGCCR	2 cut(s)	41, 255
EagI	CGGCCG	2 cut(s)	41, 255
EciI	GGCGGA	2 cut(s)	39, 285
EclXI	CGGCCG	2 cut(s)	41, 255
Eco130I	CCWWGG	1 cut(s)	1014
Eco24I	GRGCYC	1 cut(s)	290
Eco47I	GGWCC	1 cut(s)	191
Eco52I	CGGCCG	2 cut(s)	41, 255
Eco57I	CTGAAG	1 cut(s)	732
Eco88I	CYCGRG	1 cut(s)	960
Eco91I	GGTNACC	1 cut(s)	1087
EcoO109I	RGGNCCY	1 cut(s)	287
EcoO65I	GGTNACC	1 cut(s)	1087
EcoRII	CCWGG	1 cut(s)	151
EcoT14I	CCWWGG	1 cut(s)	1014
EcoT38I	GRGCYC	1 cut(s)	290
ErhI	CCWWGG	1 cut(s)	1014
FaeI	CATG	4 cut(s)	286, 511, 994, 1000
FalI	AAGNNNNNCTT	2 cut(s)	545, 577
FatI	CATG	4 cut(s)	282, 507, 990, 996
FauI	CCCGC	3 cut(s)	126, 266, 816
FbaI	TGATCA	1 cut(s)	907
Fnu4HI	GCNGC	6 cut(s)	44, 245, 271, 483, 486, 869
FokI	GGATG	2 cut(s)	48, 449
FriOI	GRGCYC	1 cut(s)	290
Fsp4HI	GCNGC	6 cut(s)	44, 245, 271, 483, 486, 869
GlaI	GCGC	1 cut(s)	22
GluI	GCNGC	6 cut(s)	44, 245, 271, 483, 486, 869
HaeII	RGCGCY	1 cut(s)	24
HaeIII	GGCC	5 cut(s)	43, 59, 156, 257, 288
HhaI	GCGC	1 cut(s)	23
Hin1II	CATG	4 cut(s)	286, 511, 994, 1000
Hin6I	GCGC	1 cut(s)	21
HinP1I	GCGC	1 cut(s)	21
HincII	GTYRAC	1 cut(s)	666
HindII	GTYRAC	1 cut(s)	666
HindIII	AAGCTT	1 cut(s)	559
HinfI	GANTC	4 cut(s)	6, 416, 445, 743
HphI	GGTGA	7 cut(s)	99, 689, 715, 746, 821, 917, 1099
Hpy166II	GTNNAC	2 cut(s)	666, 737
Hpy188I	TCNGA	6 cut(s)	11, 375, 748, 774, 955, 1023
Hpy188III	TCNNGA	6 cut(s)	391, 409, 624, 724, 962, 1030
Hpy8I	GTNNAC	2 cut(s)	666, 737
HpyAV	CCTTC	8 cut(s)	356, 406, 478, 630, 700, 725, 793, 896
HpyCH4III	ACNGT	4 cut(s)	382, 505, 938, 973
HpyCH4IV	ACGT	1 cut(s)	739
HpyCH4V	TGCA	6 cut(s)	230, 530, 871, 896, 996, 1002
HpyF10VI	GCNNNNNNNGC	4 cut(s)	27, 43, 49, 1106
HpyF3I	CTNAG	3 cut(s)	566, 771, 1022
HpySE526I	ACGT	1 cut(s)	739
Hsp92II	CATG	4 cut(s)	286, 511, 994, 1000
HspAI	GCGC	1 cut(s)	21
KpnI	GGTACC	1 cut(s)	265
KroI	GCCGGC	1 cut(s)	984
KroNI	GCCGGC	1 cut(s)	986
Ksp22I	TGATCA	1 cut(s)	907
KspI	CCGCGG	1 cut(s)	271
Kzo9I	GATC	4 cut(s)	633, 774, 876, 907
LmnI	GCTCC	4 cut(s)	340, 361, 981, 1097
Lsp1109I	GCAGC	2 cut(s)	469, 855
LweI	GCATC	4 cut(s)	379, 469, 517, 1031
MaeII	ACGT	1 cut(s)	739
MaeIII	GTNAC	4 cut(s)	92, 382, 677, 1087
MalI	GATC	4 cut(s)	635, 776, 878, 909
MboI	GATC	4 cut(s)	633, 774, 876, 907
MboII	GAAGA	2 cut(s)	388, 536
MfeI	CAATTG	1 cut(s)	304
MflI	RGATCY	1 cut(s)	876
MhlI	GDGCHC	4 cut(s)	290, 337, 358, 978
MluCI	AATT	4 cut(s)	179, 304, 754, 942
MlyI	GAGTC	1 cut(s)	410
MroNI	GCCGGC	1 cut(s)	984
MseI	TTAA	2 cut(s)	537, 576
MslI	CAYNNNNRTG	1 cut(s)	995
MspA1I	CMGCKG	1 cut(s)	270
MspR9I	CCNGG	8 cut(s)	153, 259, 300, 315, 351, 450, 492, 763
MunI	CAATTG	1 cut(s)	304
Mva1269I	GAATGC	2 cut(s)	230, 898
MvaI	CCWGG	1 cut(s)	153
MvnI	CGCG	1 cut(s)	270
MwoI	GCNNNNNNNGC	4 cut(s)	27, 43, 49, 1106
NaeI	GCCGGC	1 cut(s)	986
NciI	CCSGG	7 cut(s)	259, 300, 315, 351, 450, 492, 763
NdeII	GATC	4 cut(s)	633, 774, 876, 907
NgoMIV	GCCGGC	1 cut(s)	984
NlaIII	CATG	4 cut(s)	286, 511, 994, 1000
NlaIV	GGNNCC	6 cut(s)	38, 103, 263, 288, 312, 1028
NmuCI	GTSAC	2 cut(s)	677, 1087
NspI	RCATGY	2 cut(s)	994, 1000
PaeR7I	CTCGAG	1 cut(s)	960
PciI	ACATGT	1 cut(s)	990
PctI	GAATGC	2 cut(s)	230, 898
PdiI	GCCGGC	1 cut(s)	986
PfeI	GAWTC	3 cut(s)	6, 445, 743
PkrI	GCNGC	6 cut(s)	45, 246, 272, 484, 487, 870
PleI	GAGTC	1 cut(s)	410
PpsI	GAGTC	1 cut(s)	410
PscI	ACATGT	1 cut(s)	990
PshAI	GACNNNNGTC	1 cut(s)	594
Psp6I	CCWGG	1 cut(s)	151
PspEI	GGTNACC	1 cut(s)	1087
PspGI	CCWGG	1 cut(s)	151
PspN4I	GGNNCC	6 cut(s)	38, 103, 263, 288, 312, 1028
PspOMI	GGGCCC	1 cut(s)	286
PspPI	GGNCC	4 cut(s)	58, 191, 286, 287
PstI	CTGCAG	1 cut(s)	873
PsuI	RGATCY	1 cut(s)	876
RsaI	GTAC	5 cut(s)	161, 224, 263, 323, 815
RsaNI	GTAC	5 cut(s)	160, 223, 262, 322, 814
RseI	CAYNNNNRTG	1 cut(s)	995
SacII	CCGCGG	1 cut(s)	271
SaqAI	TTAA	2 cut(s)	537, 576
SatI	GCNGC	6 cut(s)	44, 245, 271, 483, 486, 869
Sau3AI	GATC	4 cut(s)	633, 774, 876, 907
Sau96I	GGNCC	4 cut(s)	58, 191, 286, 287
ScaI	AGTACT	1 cut(s)	224
SchI	GAGTC	1 cut(s)	410
ScrFI	CCNGG	8 cut(s)	153, 259, 300, 315, 351, 450, 492, 763
SduI	GDGCHC	4 cut(s)	290, 337, 358, 978
SfaNI	GCATC	4 cut(s)	379, 469, 517, 1031
SfcI	CTRYAG	2 cut(s)	869, 1051
Sfr274I	CTCGAG	1 cut(s)	960
Sfr303I	CCGCGG	1 cut(s)	271
SgrBI	CCGCGG	1 cut(s)	271
SinI	GGWCC	1 cut(s)	191
SlaI	CTCGAG	1 cut(s)	960
SmiMI	CAYNNNNRTG	1 cut(s)	995
SmlI	CTYRAG	1 cut(s)	960
SmoI	CTYRAG	1 cut(s)	960
Sse9I	AATT	4 cut(s)	179, 304, 754, 942
StyD4I	CCNGG	8 cut(s)	151, 257, 298, 313, 349, 448, 490, 761
StyI	CCWWGG	1 cut(s)	1014
TaaI	ACNGT	4 cut(s)	382, 505, 938, 973
TaiI	ACGT	1 cut(s)	742
TaqI	TCGA	2 cut(s)	636, 961
TasI	AATT	4 cut(s)	179, 304, 754, 942
TatI	WGTACW	2 cut(s)	222, 813
TauI	GCSGC	4 cut(s)	46, 247, 273, 488
TfiI	GAWTC	3 cut(s)	6, 445, 743
Tru1I	TTAA	2 cut(s)	537, 576
Tru9I	TTAA	2 cut(s)	537, 576
TscAI	CASTG	2 cut(s)	792, 1009
TseFI	GTSAC	2 cut(s)	677, 1087
TseI	GCWGC	2 cut(s)	482, 868
Tsp45I	GTSAC	2 cut(s)	677, 1087
TspDTI	ATGAA	3 cut(s)	271, 744, 936
TspGWI	ACGGA	1 cut(s)	204
TspRI	CASTG	2 cut(s)	792, 1009
VpaK11BI	GGWCC	1 cut(s)	191
XapI	RAATTY	1 cut(s)	754
XceI	RCATGY	2 cut(s)	994, 1000
XhoI	CTCGAG	1 cut(s)	960
ZrmI	AGTACT	1 cut(s)	224

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.