MD11G1154200.v1.1

Phospholipid diacylglycerol acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0011775
Physical Location & Seq
Reverse (-)
14771643 .. 14775591
3949 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1154200.v1.1.491

Sequence Viewer

Length: 2043 bp
ATGGCTTCGAGATCGATTCTCCGGTTTCGAAAACTCTGCTACGTTGAGCCTGTGAGCTCCCCTTCTGTAAAGTTTCGATCTTTTCAGACCTCAGAAGTTGAAGGGACAGATGAGAGTTCCGTGGAGAAGAAGCAATGGAAGAGGAAGAAGCAGCAGAGGGGGTGGAGGTGCATAGATAGCTGCTGTTGGGTCATTGGGTACATGTGCACTGCTTGGTGGGTTCTTTTGTTATTGTACCACAGCTTGCCGGTCACTTTGACCGGCTTTCAGGTGCCGGAATCTCCAGGGACAAGGCTTAAAGGTGAAGGCCTGACTGCCCTTCACCCTGTTGTTCTTGTCCCCGGCATTGTGACCGGTGGCCTCGAGCTCTGGGAAGGCAGGCCTTGTGCCGAGGGGCTTTTTCGAAAGCGGCTTTGGGGTGGTGGTTTCACCGAAATCTTCAAAAGGCCTTTGTGTTGGTTAGAGCATCTGTCTCTGCACAATGAAACGGGGCTCGATCCTCCGGGAATTCGAGTCAGGGCAGTTCCGGGACTTGTTGCAGCTGACTATTTTGCTCCTGGATATTTTGTTTGGGCTGTTTTGATCGAAAACTTGGCGAAAATTGGTTACGAAGGGAAGAATATGCATATGTCTGCGTATGATTGGCGGCTATCTTTCCAAAATACAGAGATTCGGGACCAAGCTCTTAGTAGATTGAAGAGTAAAATTGAGCTTATGTACGTAACGAATGGATATAAGAAAGTGGTTGTGGTGCCTCATTCTATGGGTGCAATTTATTTTCTCCACTTCATGAAATGGGTTGAATCACCTCCTCCTATAGGTGGTGGTGGTGGTCCAAGCTGGTGTGCCAAGCACATTAAAGCAATCATGAATATCGGTCCAGCATTTCTTGGTGTTCCAAAAGCTGTTAGTAATATATTTTCTGCTGAGGGTAAAGATGTCGCGTACATCAGAGCTATGGCTCCGGGTGTTTTAGATTCTGAATTTCTTGGGCTTCAAACCTTTGAGCACGTCATGCGGGCAAGTCGAACCTGGGATTCCATAGTTTCCATATTGCCAAAAGGTGGAGATGCCATCTGGGGCAATTTGGATTGGTCTCCTGAAGATGGGCAGAGCTGTTACCAGGCAAGGAAAGGATATCTACAGTCCACTACTGGTGACAACAATTTCAGCCGCAACGATGGAAATAGTGTTTTCCAAGTAAAAGAACCTGTGAAATATGGAAGAATAATCTCTTTTGGCAAGGAATCTTCACAATTATCTTCTTCACAGCTTCCTAGGCTTGATTTGAAGGATCTAATACACAAAGATTTGGCCACAAATTCCAAGTCATCGTGTGGAGCGGTGTGGACTGAATATGATGAGGTAAGCAGTGAAAGCATCAGAAGAATTACAGAAAATAAGGCTTACACAGCTCAAACACTTTTTGATCTACTACGTTTTGTGGCTCCAAAATTGATGCGACGGGCTGAGGCTCACTTCTCTCATGGGATAGCTGATAACCTTGATGATCCTAAATACGCCCATTACAAGTACTGGTCCAATCCACTAGAGACCAAGTTACCGGTTGCTCCAAACATGGAAATATACTGTTTATATGGCGTTGGAATCCCCACTGAAAGATCATATGTATACAAGATGTCTCCTTCTAACAAGTGCAAAAGCATTCCATTCCGGATTGATGGTTCAGCAGATGGAGAGGCAGGAAGTTGTCTGAAAAATGGAATACATTTTGTGGATGGTGACGATAGTGTGCCTGTATTGAGTGCAGGTTTCATGTGTGCCAAAGGATGGAGAGGAAGAACGAGATTCAACCCATCTGGTATTGCTACTTACATAAGGGAGTACCGGCACAAGCCACCGGCTAGTTTCCTTGAGGGGAGGGGTATAGAGAGTGGTGCACATGTTGACATCATGGGAAACGTTGCATTAATTGAAGATGTTCTCAGGGTTGCTGCCGGAGCAAATGGTGCACAGACCGGAGGTGATAGGATTTACTCAGATATCATGAGAATATCTGAGAGAATAAATCTACAGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 map00561
Pfam Domains
Protein Families
LCAT Lipase_GDSL

Protein Analysis

681

Amino Acids

75.82

Weight (kDa)

8.9

Isoelectric Point (pI)

49.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCAT PF02450 134 - 640 2.3e-63 Lecithin:cholesterol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011775)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G44830
fragaria_vesca FvH4_3g30050
malus_domestica MD11G1154200.v1.1
prunus_persica Prupe.6G116700_v2.0.a1 Prupe.6G116700_v2.0.a1
pyrus_communis pycom11g12590
rosa_chinensis RchiOBHm_Chr5g0055851 RchiOBHm_Chr5g0055861
rosa_laevigata RLG00000035014
rosa_multiflora Rmu_sc0003306.1_g000008
rosa_roxburghii Rroxscaffold_1G00024510
rosa_rugosa Rorug05G0295200 Rorug05G0295200 Rorug05G0295300
rosa_samantha Rh5AG366600 Rh5BG376800 Rh5CG399800 Rh5DG389800
rosa_wichuraiana Rw5G034360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1760
AccB1I GGYRCC 2 cut(s) 271, 751
AccB7I CCANNNNNTGG 2 cut(s) 1064, 1791
AccBSI CCGCTC 1 cut(s) 1343
AccI GTMKAC 1 cut(s) 1632
AccII CGCG 1 cut(s) 944
AccIII TCCGGA 1 cut(s) 1674
AciI CCGC 5 cut(s) 409, 646, 1018, 1174, 1343
AclI AACGTT 1 cut(s) 1923
AclWI GGATC 3 cut(s) 491, 1302, 1505
AcoI YGGCCR 1 cut(s) 1314
AcsI RAATTY 3 cut(s) 507, 983, 1321
AcuI CTGAAG 1 cut(s) 1122
AfaI GTAC 6 cut(s) 200, 236, 719, 947, 1535, 1847
AfiI CCNNNNNNNGG 5 cut(s) 818, 821, 1064, 1106, 1791
AflIII ACRYGT 2 cut(s) 201, 1903
AgeI ACCGGT 2 cut(s) 353, 1564
AgsI TTSAA 8 cut(s) 101, 442, 697, 803, 998, 1291, 1813, 1937
AjiI CACGTC 1 cut(s) 1012
AjnI CCWGG 4 cut(s) 283, 556, 1031, 1122
AjuI GAANNNNNNNTTGG 2 cut(s) 397, 429
AloI GAACNNNNNNTCC 2 cut(s) 1669, 1701
Alw21I GWGCWC 6 cut(s) 59, 209, 369, 1011, 1903, 1975
Alw26I GTCTC 4 cut(s) 477, 1101, 1547, 1647
Alw44I GTGCAC 3 cut(s) 205, 1899, 1971
AlwI GGATC 3 cut(s) 491, 1302, 1505
Ama87I CYCGRG 1 cut(s) 362
Aor13HI TCCGGA 1 cut(s) 1674
AoxI GGCC 5 cut(s) 307, 358, 380, 446, 1314
ApaLI GTGCAC 3 cut(s) 205, 1899, 1971
ApeKI GCWGC 4 cut(s) 151, 180, 539, 1955
ApoI RAATTY 3 cut(s) 507, 983, 1321
ArsI GACNNNNNNTTYG 2 cut(s) 1313, 1345
AseI ATTAAT 1 cut(s) 1931
AsiGI ACCGGT 2 cut(s) 353, 1564
Asp700I GAANNNNTTC 1 cut(s) 1941
AspA2I CCTAGG 1 cut(s) 1277
AspS9I GGNCC 4 cut(s) 676, 833, 878, 1539
AsuC2I CCSGG 4 cut(s) 342, 504, 528, 966
AsuHPI GGTGA 7 cut(s) 314, 314, 421, 798, 1169, 1754, 1997
AsuII TTCGAA 2 cut(s) 28, 403
AvaI CYCGRG 1 cut(s) 362
AvaII GGWCC 4 cut(s) 676, 833, 878, 1539
AvrII CCTAGG 1 cut(s) 1277
BaeGI GKGCMC 3 cut(s) 209, 1903, 1975
BalI TGGCCA 1 cut(s) 1316
BanI GGYRCC 2 cut(s) 271, 751
BanII GRGCYC 3 cut(s) 59, 369, 495
Bbv12I GWGCWC 6 cut(s) 59, 209, 369, 1011, 1903, 1975
BbvCI CCTCAGC 2 cut(s) 927, 1470
BbvI GCAGC 4 cut(s) 163, 167, 551, 1942
BccI CCATC 8 cut(s) 1082, 1100, 1175, 1676, 1688, 1733, 1785, 1825
BcgI CGANNNNNNTGC 2 cut(s) 18, 52
BciT130I CCWGG 4 cut(s) 285, 558, 1033, 1124
BcnI CCSGG 4 cut(s) 342, 504, 528, 966
BcoDI GTCTC 4 cut(s) 477, 1101, 1547, 1647
BfaI CTAG 3 cut(s) 1278, 1550, 1866
BfmI CTRYAG 3 cut(s) 816, 1142, 2033
BfuAI ACCTGC 1 cut(s) 1760
BisI GCNGC 7 cut(s) 152, 181, 410, 540, 647, 1174, 1956
BlnI CCTAGG 1 cut(s) 1277
BlsI GCNGC 7 cut(s) 153, 182, 411, 541, 648, 1175, 1957
BmcAI AGTACT 1 cut(s) 1535
Bme1390I CCNGG 8 cut(s) 285, 342, 504, 528, 558, 966, 1033, 1124
Bme18I GGWCC 4 cut(s) 676, 833, 878, 1539
BmeT110I CYCGRG 1 cut(s) 362
BmgBI CACGTC 1 cut(s) 1012
BmgT120I GGNCC 4 cut(s) 676, 833, 878, 1539
BmiI GGNNCC 5 cut(s) 273, 677, 753, 963, 1449
BmrFI CCNGG 8 cut(s) 285, 342, 504, 528, 558, 966, 1033, 1124
BmsI GCATC 4 cut(s) 475, 1060, 1389, 1449
BpmI CTGGAG 1 cut(s) 267
Bpu10I CCTNAGC 2 cut(s) 927, 1470
Bpu14I TTCGAA 2 cut(s) 28, 403
BpuEI CTTGAG 1 cut(s) 1895
BpuMI CCSGG 4 cut(s) 342, 504, 528, 966
Bsa29I ATCGAT 1 cut(s) 14
BsaAI YACGTR 1 cut(s) 721
BsaI GGTCTC 2 cut(s) 1101, 1547
BsaJI CCNNGG 6 cut(s) 120, 284, 340, 390, 1032, 1277
BsaWI WCCGGW 5 cut(s) 21, 353, 1564, 1674, 1979
Bsc4I CCNNNNNNNGG 5 cut(s) 818, 821, 1064, 1106, 1791
Bse118I RCCGGY 6 cut(s) 247, 260, 353, 1564, 1848, 1861
Bse1I ACTGG 2 cut(s) 1159, 1541
Bse3DI GCAATG 1 cut(s) 140
BseAI TCCGGA 1 cut(s) 1674
BseBI CCWGG 4 cut(s) 285, 558, 1033, 1124
BseCI ATCGAT 1 cut(s) 14
BseDI CCNNGG 6 cut(s) 120, 284, 340, 390, 1032, 1277
BseGI GGATG 2 cut(s) 1744, 1796
BseLI CCNNNNNNNGG 5 cut(s) 818, 821, 1064, 1106, 1791
BseMI GCAATG 1 cut(s) 140
BseMII CTCAG 6 cut(s) 105, 918, 1461, 1960, 2010, 2013
BseNI ACTGG 2 cut(s) 1159, 1541
BseRI GAGGAG 1 cut(s) 801
BseSI GKGCMC 3 cut(s) 209, 1903, 1975
BseXI GCAGC 4 cut(s) 163, 167, 551, 1942
BsgI GTGCAG 2 cut(s) 461, 1788
Bsh1236I CGCG 1 cut(s) 944
BshFI GGCC 5 cut(s) 309, 360, 382, 448, 1316
BshNI GGYRCC 2 cut(s) 271, 751
BshTI ACCGGT 2 cut(s) 353, 1564
BshVI ATCGAT 1 cut(s) 14
BsiHKAI GWGCWC 6 cut(s) 59, 209, 369, 1011, 1903, 1975
BsiHKCI CYCGRG 1 cut(s) 362
BslFI GGGAC 5 cut(s) 118, 301, 323, 543, 689
BslI CCNNNNNNNGG 5 cut(s) 818, 821, 1064, 1106, 1791
BsmAI GTCTC 4 cut(s) 477, 1101, 1547, 1647
BsmFI GGGAC 5 cut(s) 118, 301, 323, 543, 689
BsmI GAATGC 1 cut(s) 1665
BsnI GGCC 5 cut(s) 309, 360, 382, 448, 1316
Bso31I GGTCTC 2 cut(s) 1101, 1547
BsoBI CYCGRG 1 cut(s) 362
Bsp119I TTCGAA 2 cut(s) 28, 403
Bsp1286I GDGCHC 7 cut(s) 59, 209, 369, 495, 1011, 1903, 1975
Bsp13I TCCGGA 1 cut(s) 1674
Bsp143I GATC 8 cut(s) 11, 77, 496, 582, 1294, 1429, 1510, 1622
BspACI CCGC 5 cut(s) 409, 646, 1018, 1174, 1343
BspANI GGCC 5 cut(s) 309, 360, 382, 448, 1316
BspCNI CTCAG 6 cut(s) 104, 919, 1462, 1959, 2011, 2012
BspDI ATCGAT 1 cut(s) 14
BspEI TCCGGA 1 cut(s) 1674
BspFNI CGCG 1 cut(s) 944
BspHI TCATGA 3 cut(s) 789, 867, 2007
BspLI GGNNCC 5 cut(s) 273, 677, 753, 963, 1449
BspMI ACCTGC 1 cut(s) 1760
BspPI GGATC 3 cut(s) 491, 1302, 1505
BspT104I TTCGAA 2 cut(s) 28, 403
BspT107I GGYRCC 2 cut(s) 271, 751
BspTNI GGTCTC 2 cut(s) 1101, 1547
BsrBI CCGCTC 1 cut(s) 1343
BsrDI GCAATG 1 cut(s) 140
BsrFI RCCGGY 6 cut(s) 247, 260, 353, 1564, 1848, 1861
BsrI ACTGG 2 cut(s) 1159, 1541
BssAI RCCGGY 6 cut(s) 247, 260, 353, 1564, 1848, 1861
BssECI CCNNGG 6 cut(s) 120, 284, 340, 390, 1032, 1277
BssMI GATC 8 cut(s) 11, 77, 496, 582, 1294, 1429, 1510, 1622
BssNAI GTATAC 1 cut(s) 1633
BssT1I CCWWGG 1 cut(s) 1277
Bst1107I GTATAC 1 cut(s) 1633
Bst2UI CCWGG 4 cut(s) 285, 558, 1033, 1124
Bst4CI ACNGT 2 cut(s) 1146, 1592
Bst6I CTCTTC 2 cut(s) 134, 692
BstAPI GCANNNNNTGC 2 cut(s) 1015, 1970
BstBAI YACGTR 1 cut(s) 721
BstBI TTCGAA 2 cut(s) 28, 403
BstC8I GCNNGC 3 cut(s) 245, 380, 1020
BstDEI CTNAG 7 cut(s) 91, 686, 927, 1470, 1946, 1999, 2019
BstDSI CCRYGG 1 cut(s) 120
BstENI CCTNNNNNAGG 1 cut(s) 816
BstF5I GGATG 2 cut(s) 1744, 1796
BstFNI CGCG 1 cut(s) 944
BstKTI GATC 8 cut(s) 14, 80, 499, 585, 1297, 1432, 1513, 1625
BstMAI GTCTC 4 cut(s) 477, 1101, 1547, 1647
BstMBI GATC 8 cut(s) 11, 77, 496, 582, 1294, 1429, 1510, 1622
BstMWI GCNNNNNNNGC 7 cut(s) 177, 1015, 1279, 1377, 1412, 1961, 1970
BstNI CCWGG 4 cut(s) 285, 558, 1033, 1124
BstNSI RCATGY 2 cut(s) 205, 1907
BstSCI CCNGG 8 cut(s) 283, 340, 502, 526, 556, 964, 1031, 1122
BstSFI CTRYAG 3 cut(s) 816, 1142, 2033
BstSLI GKGCMC 3 cut(s) 209, 1903, 1975
BstSNI TACGTA 1 cut(s) 721
BstUI CGCG 1 cut(s) 944
BstV1I GCAGC 4 cut(s) 163, 167, 551, 1942
BstX2I RGATCY 1 cut(s) 1294
BstYI RGATCY 1 cut(s) 1294
BstZ17I GTATAC 1 cut(s) 1633
Bsu15I ATCGAT 1 cut(s) 14
BsuRI GGCC 5 cut(s) 309, 360, 382, 448, 1316
BsuTUI ATCGAT 1 cut(s) 14
BtgI CCRYGG 1 cut(s) 120
BtrI CACGTC 1 cut(s) 1012
BtsCI GGATG 2 cut(s) 1744, 1796
BtsI GCAGTG 2 cut(s) 207, 1378
BtsIMutI CAGTG 3 cut(s) 207, 1378, 1614
BveI ACCTGC 1 cut(s) 1760
Cac8I GCNNGC 3 cut(s) 245, 380, 1020
CciI TCATGA 3 cut(s) 789, 867, 2007
Cfr10I RCCGGY 6 cut(s) 247, 260, 353, 1564, 1848, 1861
Cfr13I GGNCC 4 cut(s) 676, 833, 878, 1539
ClaI ATCGAT 1 cut(s) 14
Csp6I GTAC 6 cut(s) 199, 235, 718, 946, 1534, 1846
CspAI ACCGGT 2 cut(s) 353, 1564
CviQI GTAC 6 cut(s) 199, 235, 718, 946, 1534, 1846
DdeI CTNAG 7 cut(s) 91, 686, 927, 1470, 1946, 1999, 2019
DpnI GATC 8 cut(s) 13, 79, 498, 584, 1296, 1431, 1512, 1624
DpnII GATC 8 cut(s) 11, 77, 496, 582, 1294, 1429, 1510, 1622
EaeI YGGCCR 1 cut(s) 1314
Eam1104I CTCTTC 2 cut(s) 134, 692
EarI CTCTTC 2 cut(s) 134, 692
Ecl136II GAGCTC 2 cut(s) 57, 367
Eco105I TACGTA 1 cut(s) 721
Eco130I CCWWGG 1 cut(s) 1277
Eco147I AGGCCT 3 cut(s) 309, 382, 448
Eco24I GRGCYC 3 cut(s) 59, 369, 495
Eco31I GGTCTC 2 cut(s) 1101, 1547
Eco32I GATATC 2 cut(s) 1139, 2005
Eco47I GGWCC 4 cut(s) 676, 833, 878, 1539
Eco53kI GAGCTC 2 cut(s) 57, 367
Eco57I CTGAAG 1 cut(s) 1122
Eco88I CYCGRG 1 cut(s) 362
EcoICRI GAGCTC 2 cut(s) 57, 367
EcoNI CCTNNNNNAGG 1 cut(s) 816
EcoRI GAATTC 1 cut(s) 507
EcoRII CCWGG 4 cut(s) 283, 556, 1031, 1122
EcoRV GATATC 2 cut(s) 1139, 2005
EcoT14I CCWWGG 1 cut(s) 1277
EcoT22I ATGCAT 1 cut(s) 627
EcoT38I GRGCYC 3 cut(s) 59, 369, 495
ErhI CCWWGG 1 cut(s) 1277
FaqI GGGAC 5 cut(s) 118, 301, 323, 543, 689
FauI CCCGC 1 cut(s) 1011
FauNDI CATATG 2 cut(s) 627, 1627
FblI GTMKAC 1 cut(s) 1632
Fnu4HI GCNGC 7 cut(s) 152, 181, 410, 540, 647, 1174, 1956
FokI GGATG 2 cut(s) 1751, 1803
FriOI GRGCYC 3 cut(s) 59, 369, 495
Fsp4HI GCNGC 7 cut(s) 152, 181, 410, 540, 647, 1174, 1956
FspBI CTAG 3 cut(s) 1278, 1550, 1866
GluI GCNGC 7 cut(s) 152, 181, 410, 540, 647, 1174, 1956
GsuI CTGGAG 1 cut(s) 267
HaeIII GGCC 5 cut(s) 309, 360, 382, 448, 1316
HincII GTYRAC 1 cut(s) 1909
HindII GTYRAC 1 cut(s) 1909
HphI GGTGA 7 cut(s) 314, 314, 421, 798, 1169, 1754, 1997
Hpy166II GTNNAC 7 cut(s) 207, 1149, 1350, 1633, 1901, 1909, 1973
Hpy188I TCNGA 8 cut(s) 87, 94, 953, 982, 1385, 1716, 2002, 2020
Hpy188III TCNNGA 7 cut(s) 9, 674, 790, 868, 1100, 1675, 2008
Hpy8I GTNNAC 7 cut(s) 207, 1149, 1350, 1633, 1901, 1909, 1973
Hpy99I CGWCG 1 cut(s) 1467
HpyAV CCTTC 8 cut(s) 72, 95, 299, 329, 368, 605, 1285, 1656
HpyCH4III ACNGT 2 cut(s) 1146, 1592
HpyCH4IV ACGT 5 cut(s) 42, 720, 1011, 1438, 1923
HpyF10VI GCNNNNNNNGC 7 cut(s) 177, 1015, 1279, 1377, 1412, 1961, 1970
HpyF3I CTNAG 7 cut(s) 91, 686, 927, 1470, 1946, 1999, 2019
HpySE526I ACGT 5 cut(s) 42, 720, 1011, 1438, 1923
Kpn2I TCCGGA 1 cut(s) 1674
Kzo9I GATC 8 cut(s) 11, 77, 496, 582, 1294, 1429, 1510, 1622
LmnI GCTCC 7 cut(s) 62, 559, 967, 1340, 1453, 1576, 1961
Lsp1109I GCAGC 4 cut(s) 163, 167, 551, 1942
LweI GCATC 4 cut(s) 475, 1060, 1389, 1449
MaeI CTAG 3 cut(s) 1278, 1550, 1866
MaeII ACGT 5 cut(s) 42, 720, 1011, 1438, 1923
MaeIII GTNAC 8 cut(s) 250, 349, 605, 721, 1118, 1157, 1560, 1742
MalI GATC 8 cut(s) 13, 79, 498, 584, 1296, 1431, 1512, 1624
MbiI CCGCTC 1 cut(s) 1343
MboI GATC 8 cut(s) 11, 77, 496, 582, 1294, 1429, 1510, 1622
MflI RGATCY 1 cut(s) 1294
MhlI GDGCHC 7 cut(s) 59, 209, 369, 495, 1011, 1903, 1975
MlsI TGGCCA 1 cut(s) 1316
MluNI TGGCCA 1 cut(s) 1316
MlyI GAGTC 1 cut(s) 522
MmeI TCCRAC 1 cut(s) 1585
Mox20I TGGCCA 1 cut(s) 1316
Mph1103I ATGCAT 1 cut(s) 627
MroI TCCGGA 1 cut(s) 1674
MroXI GAANNNNTTC 1 cut(s) 1941
MscI TGGCCA 1 cut(s) 1316
MseI TTAA 3 cut(s) 297, 858, 1931
Msp20I TGGCCA 1 cut(s) 1316
MspA1I CMGCKG 1 cut(s) 542
MspR9I CCNGG 8 cut(s) 285, 342, 504, 528, 558, 966, 1033, 1124
Mva1269I GAATGC 1 cut(s) 1665
MvaI CCWGG 4 cut(s) 285, 558, 1033, 1124
MvnI CGCG 1 cut(s) 944
MwoI GCNNNNNNNGC 7 cut(s) 177, 1015, 1279, 1377, 1412, 1961, 1970
NciI CCSGG 4 cut(s) 342, 504, 528, 966
NdeI CATATG 2 cut(s) 627, 1627
NdeII GATC 8 cut(s) 11, 77, 496, 582, 1294, 1429, 1510, 1622
NlaIV GGNNCC 5 cut(s) 273, 677, 753, 963, 1449
NmeAIII GCCGAG 1 cut(s) 415
NmuCI GTSAC 4 cut(s) 250, 349, 1157, 1742
NsiI ATGCAT 1 cut(s) 627
NspI RCATGY 2 cut(s) 205, 1907
NspV TTCGAA 2 cut(s) 28, 403
PaeR7I CTCGAG 1 cut(s) 362
PagI TCATGA 3 cut(s) 789, 867, 2007
PceI AGGCCT 3 cut(s) 309, 382, 448
PciI ACATGT 2 cut(s) 201, 1903
PctI GAATGC 1 cut(s) 1665
PdmI GAANNNNTTC 1 cut(s) 1941
PfeI GAWTC 9 cut(s) 16, 278, 670, 803, 977, 1037, 1247, 1608, 1809
PflMI CCANNNNNTGG 2 cut(s) 1064, 1791
PfoI TCCNGGA 3 cut(s) 502, 526, 556
PinAI ACCGGT 2 cut(s) 353, 1564
PkrI GCNGC 7 cut(s) 153, 182, 411, 541, 648, 1175, 1957
PleI GAGTC 1 cut(s) 521
PpsI GAGTC 1 cut(s) 521
Ppu21I YACGTR 1 cut(s) 721
PscI ACATGT 2 cut(s) 201, 1903
PshBI ATTAAT 1 cut(s) 1931
Psp124BI GAGCTC 2 cut(s) 59, 369
Psp1406I AACGTT 1 cut(s) 1923
Psp6I CCWGG 4 cut(s) 283, 556, 1031, 1122
PspGI CCWGG 4 cut(s) 283, 556, 1031, 1122
PspN4I GGNNCC 5 cut(s) 273, 677, 753, 963, 1449
PspPI GGNCC 4 cut(s) 676, 833, 878, 1539
PspXI VCTCGAGB 1 cut(s) 362
PsuI RGATCY 1 cut(s) 1294
PvuII CAGCTG 1 cut(s) 542
RsaI GTAC 6 cut(s) 200, 236, 719, 947, 1535, 1847
RsaNI GTAC 6 cut(s) 199, 235, 718, 946, 1534, 1846
SacI GAGCTC 2 cut(s) 59, 369
SaqAI TTAA 3 cut(s) 297, 858, 1931
SatI GCNGC 7 cut(s) 152, 181, 410, 540, 647, 1174, 1956
Sau3AI GATC 8 cut(s) 11, 77, 496, 582, 1294, 1429, 1510, 1622
Sau96I GGNCC 4 cut(s) 676, 833, 878, 1539
ScaI AGTACT 1 cut(s) 1535
SchI GAGTC 1 cut(s) 522
ScrFI CCNGG 8 cut(s) 285, 342, 504, 528, 558, 966, 1033, 1124
SduI GDGCHC 7 cut(s) 59, 209, 369, 495, 1011, 1903, 1975
SfaNI GCATC 4 cut(s) 475, 1060, 1389, 1449
SfcI CTRYAG 3 cut(s) 816, 1142, 2033
Sfr274I CTCGAG 1 cut(s) 362
SfuI TTCGAA 2 cut(s) 28, 403
SinI GGWCC 4 cut(s) 676, 833, 878, 1539
SlaI CTCGAG 1 cut(s) 362
SmlI CTYRAG 2 cut(s) 362, 1874
SmoI CTYRAG 2 cut(s) 362, 1874
SnaBI TACGTA 1 cut(s) 721
SseBI AGGCCT 3 cut(s) 309, 382, 448
SsiI CCGC 5 cut(s) 409, 646, 1018, 1174, 1343
SspMI CTAG 3 cut(s) 1278, 1550, 1866
SstI GAGCTC 2 cut(s) 59, 369
StuI AGGCCT 3 cut(s) 309, 382, 448
StyD4I CCNGG 8 cut(s) 283, 340, 502, 526, 556, 964, 1031, 1122
StyI CCWWGG 1 cut(s) 1277
TaaI ACNGT 2 cut(s) 1146, 1592
TaiI ACGT 5 cut(s) 45, 723, 1014, 1441, 1926
TaqII GACCGA 1 cut(s) 866
TatI WGTACW 1 cut(s) 1533
TauI GCSGC 3 cut(s) 412, 649, 1176
TfiI GAWTC 9 cut(s) 16, 278, 670, 803, 977, 1037, 1247, 1608, 1809
Tru1I TTAA 3 cut(s) 297, 858, 1931
Tru9I TTAA 3 cut(s) 297, 858, 1931
TscAI CASTG 3 cut(s) 214, 1378, 1621
TseFI GTSAC 4 cut(s) 250, 349, 1157, 1742
TseI GCWGC 4 cut(s) 151, 180, 539, 1955
Tsp45I GTSAC 4 cut(s) 250, 349, 1157, 1742
TspDTI ATGAA 5 cut(s) 498, 778, 806, 884, 1765
TspGWI ACGGA 1 cut(s) 109
TspRI CASTG 3 cut(s) 214, 1378, 1621
Van91I CCANNNNNTGG 2 cut(s) 1064, 1791
VneI GTGCAC 3 cut(s) 205, 1899, 1971
VpaK11BI GGWCC 4 cut(s) 676, 833, 878, 1539
VspI ATTAAT 1 cut(s) 1931
XagI CCTNNNNNAGG 1 cut(s) 816
XapI RAATTY 3 cut(s) 507, 983, 1321
XceI RCATGY 2 cut(s) 205, 1907
XhoI CTCGAG 1 cut(s) 362
XmaJI CCTAGG 1 cut(s) 1277
XmiI GTMKAC 1 cut(s) 1632
XmnI GAANNNNTTC 1 cut(s) 1941
XspI CTAG 3 cut(s) 1278, 1550, 1866
ZrmI AGTACT 1 cut(s) 1535
Zsp2I ATGCAT 1 cut(s) 627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.