RchiOBHm_Chr5g0055851

Phospholipid diacylglycerol acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0011775
Physical Location & Seq
Forward (+)
59204093 .. 59205918
1826 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33279

Sequence Viewer

Length: 1014 bp
ATGGCTGCTTCAATTCTTCGGCTTCGCAAGCTGTGCTATGTTCAGCCTGTGAGGTTTTCGGCATTGTGCTTTCAGTCCTTTGAAACAGCCAAGGACGAGGGTGCTGAAACCCCGAAGTTCGAAAATGAAGTGAAAGAGAAACAGAGGATTAAGAACAAGAACAAGAAGCAACCGAGGGAATGGAGATGCATACATAACTGTTGTTGGATAATTGGGTCCATGTGTACAACTTGGTGGCTTCTCTTGTTTTTGTACAACACCTTGCCAACTGCATTGACTGGTTTTCAGGTGCCAGAATCACCTGGAACAAGGCTTAGGCGCGAAGGTGTAAGCCCGCTTCACCCAGTTGTCATGGTCCCTGGCATTGTAACCGGTGGCCTAGAGCTCTGGGAGGGCAGGCCTTGTGCTGAAGGTCTTTTTAGGAAGCGACTTTGGGGTGGTAGTTTCTCAGAGATCTTCAAAAGGCCTATGTGTTTGTTGGAGCACCTATCACTGGACAATGAAACAGGGCTGGACCCTCCAGGAATTCGAGTCAGAGCAGTTCGGGGACTCGCTGCAGCTGACTATTTTGCTCCTGGCTACTTTGTTTGGGCTGTCCTCATTAAGAATTTGGCAAAAATTGGTTATGAAGGGAAGAACATGCATATGGCTGCCTATGATTGGAGGCTTTCTTTCCAGAACACTGAGATTCGGGACCAAGCTCTTAGTAGGTTGAAGAGTAAAATTGAGCTTATGTATGCAATCAATGGCCATAAGAAAGTGGTCGTGGTGCCTCATTCTATGGGGGCTATTTATTTTCTCCACTTCATGAAATGGGTCGAGTCACCTCCTCCTATGGGAGGTGGTGGTGGTCCAAGTTGGTGTGCCACGCACATCAAAGCAATCATGAATATCGGTCCAGCATTTCTTGGTGTTCCAAGAGCAGTTAGTAATATATTTTCTGCTGAGGGTAAAGATGTTGCTTACATCAGGTTTGTCTATGTGTTCAATTTCTCATGTGTGTTTGCATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 map00561
Pfam Domains
Protein Families
LCAT Lipase_GDSL

Protein Analysis

337

Amino Acids

38.1

Weight (kDa)

9.44

Isoelectric Point (pI)

44.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCAT PF02450 140 - 322 1.3e-39 Lecithin:cholesterol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011775)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G44830
fragaria_vesca FvH4_3g30050
malus_domestica MD11G1154200.v1.1
prunus_persica Prupe.6G116700_v2.0.a1 Prupe.6G116700_v2.0.a1
pyrus_communis pycom11g12590
rosa_chinensis RchiOBHm_Chr5g0055851 RchiOBHm_Chr5g0055861
rosa_laevigata RLG00000035014
rosa_multiflora Rmu_sc0003306.1_g000008
rosa_roxburghii Rroxscaffold_1G00024510
rosa_rugosa Rorug05G0295200 Rorug05G0295200 Rorug05G0295300
rosa_samantha Rh5AG366600 Rh5BG376800 Rh5CG399800 Rh5DG389800
rosa_wichuraiana Rw5G034360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 289, 769
AccII CGCG 1 cut(s) 321
AciI CCGC 1 cut(s) 335
AcoI YGGCCR 1 cut(s) 748
AcsI RAATTY 2 cut(s) 525, 607
AcuI CTGAAG 1 cut(s) 429
AfaI GTAC 2 cut(s) 226, 254
AfiI CCNNNNNNNGG 4 cut(s) 493, 660, 836, 839
AgeI ACCGGT 1 cut(s) 371
AgsI TTSAA 5 cut(s) 12, 83, 460, 715, 988
AjnI CCWGG 4 cut(s) 301, 358, 520, 574
AluBI AGCT 5 cut(s) 31, 385, 560, 701, 730
AluI AGCT 5 cut(s) 31, 385, 560, 701, 730
Alw21I GWGCWC 2 cut(s) 387, 486
AoxI GGCC 4 cut(s) 376, 398, 464, 748
ApeKI GCWGC 4 cut(s) 5, 554, 557, 650
ApoI RAATTY 2 cut(s) 525, 607
AsiGI ACCGGT 1 cut(s) 371
AspLEI GCGC 1 cut(s) 321
AspS9I GGNCC 6 cut(s) 216, 355, 514, 694, 851, 896
AsuHPI GGTGA 3 cut(s) 291, 332, 816
AsuII TTCGAA 1 cut(s) 120
AvaII GGWCC 6 cut(s) 216, 355, 514, 694, 851, 896
BalI TGGCCA 1 cut(s) 750
BanI GGYRCC 2 cut(s) 289, 769
BanII GRGCYC 1 cut(s) 387
BarI GAAGNNNNNNTAC 2 cut(s) 321, 353
Bbv12I GWGCWC 2 cut(s) 387, 486
BbvCI CCTCAGC 1 cut(s) 945
BbvI GCAGC 3 cut(s) 541, 569, 637
BciT130I CCWGG 4 cut(s) 303, 360, 522, 576
BfaI CTAG 1 cut(s) 380
BfmI CTRYAG 1 cut(s) 555
BglII AGATCT 1 cut(s) 453
BisI GCNGC 4 cut(s) 6, 555, 558, 651
BlsI GCNGC 4 cut(s) 7, 556, 559, 652
Bme1390I CCNGG 4 cut(s) 303, 360, 522, 576
Bme18I GGWCC 6 cut(s) 216, 355, 514, 694, 851, 896
BmgT120I GGNCC 6 cut(s) 216, 355, 514, 694, 851, 896
BmiI GGNNCC 6 cut(s) 217, 291, 357, 516, 695, 771
BmrFI CCNGG 4 cut(s) 303, 360, 522, 576
BmrI ACTGGG 1 cut(s) 338
BmsI GCATC 1 cut(s) 176
BmuI ACTGGG 1 cut(s) 338
BpmI CTGGAG 1 cut(s) 504
Bpu10I CCTNAGC 2 cut(s) 314, 945
Bpu14I TTCGAA 1 cut(s) 120
BsaJI CCNNGG 3 cut(s) 90, 173, 358
BsaWI WCCGGW 1 cut(s) 371
Bsc4I CCNNNNNNNGG 4 cut(s) 493, 660, 836, 839
Bse118I RCCGGY 1 cut(s) 371
Bse1I ACTGG 3 cut(s) 283, 344, 498
BseBI CCWGG 4 cut(s) 303, 360, 522, 576
BseDI CCNNGG 3 cut(s) 90, 173, 358
BseLI CCNNNNNNNGG 4 cut(s) 493, 660, 836, 839
BseMII CTCAG 3 cut(s) 462, 675, 936
BseNI ACTGG 3 cut(s) 283, 344, 498
BseRI GAGGAG 1 cut(s) 819
BseXI GCAGC 3 cut(s) 541, 569, 637
Bsh1236I CGCG 1 cut(s) 321
BshFI GGCC 4 cut(s) 378, 400, 466, 750
BshNI GGYRCC 2 cut(s) 289, 769
BshTI ACCGGT 1 cut(s) 371
BsiHKAI GWGCWC 2 cut(s) 387, 486
BsiSI CCGG 1 cut(s) 372
BslFI GGGAC 3 cut(s) 341, 561, 707
BslI CCNNNNNNNGG 4 cut(s) 493, 660, 836, 839
BsmFI GGGAC 3 cut(s) 341, 561, 707
BsmI GAATGC 1 cut(s) 1007
BsnI GGCC 4 cut(s) 378, 400, 466, 750
Bsp119I TTCGAA 1 cut(s) 120
Bsp1286I GDGCHC 2 cut(s) 387, 486
Bsp1407I TGTACA 2 cut(s) 224, 252
Bsp143I GATC 1 cut(s) 453
BspACI CCGC 1 cut(s) 335
BspANI GGCC 4 cut(s) 378, 400, 466, 750
BspCNI CTCAG 3 cut(s) 461, 676, 937
BspFNI CGCG 1 cut(s) 321
BspHI TCATGA 2 cut(s) 807, 885
BspLI GGNNCC 6 cut(s) 217, 291, 357, 516, 695, 771
BspMAI CTGCAG 1 cut(s) 559
BspT104I TTCGAA 1 cut(s) 120
BspT107I GGYRCC 2 cut(s) 289, 769
BsrFI RCCGGY 1 cut(s) 371
BsrGI TGTACA 2 cut(s) 224, 252
BsrI ACTGG 3 cut(s) 283, 344, 498
BssAI RCCGGY 1 cut(s) 371
BssECI CCNNGG 3 cut(s) 90, 173, 358
BssMI GATC 1 cut(s) 453
BssT1I CCWWGG 1 cut(s) 90
Bst2UI CCWGG 4 cut(s) 303, 360, 522, 576
Bst4CI ACNGT 1 cut(s) 200
Bst6I CTCTTC 1 cut(s) 710
BstAPI GCANNNNNTGC 1 cut(s) 33
BstAUI TGTACA 2 cut(s) 224, 252
BstBI TTCGAA 1 cut(s) 120
BstC8I GCNNGC 3 cut(s) 29, 335, 398
BstDEI CTNAG 5 cut(s) 314, 448, 684, 704, 945
BstENI CCTNNNNNAGG 1 cut(s) 837
BstFNI CGCG 1 cut(s) 321
BstHHI GCGC 1 cut(s) 321
BstKTI GATC 1 cut(s) 456
BstMBI GATC 1 cut(s) 453
BstMWI GCNNNNNNNGC 2 cut(s) 28, 33
BstNI CCWGG 4 cut(s) 303, 360, 522, 576
BstNSI RCATGY 1 cut(s) 643
BstSCI CCNGG 4 cut(s) 301, 358, 520, 574
BstSFI CTRYAG 1 cut(s) 555
BstUI CGCG 1 cut(s) 321
BstV1I GCAGC 3 cut(s) 541, 569, 637
BstX2I RGATCY 1 cut(s) 453
BstYI RGATCY 1 cut(s) 453
BsuRI GGCC 4 cut(s) 378, 400, 466, 750
BtsIMutI CAGTG 2 cut(s) 491, 681
Cac8I GCNNGC 3 cut(s) 29, 335, 398
CciI TCATGA 2 cut(s) 807, 885
CfoI GCGC 1 cut(s) 321
Cfr10I RCCGGY 1 cut(s) 371
Cfr13I GGNCC 6 cut(s) 216, 355, 514, 694, 851, 896
Csp6I GTAC 2 cut(s) 225, 253
CspAI ACCGGT 1 cut(s) 371
CviAII CATG 6 cut(s) 220, 352, 640, 808, 886, 996
CviQI GTAC 2 cut(s) 225, 253
DdeI CTNAG 5 cut(s) 314, 448, 684, 704, 945
DpnI GATC 1 cut(s) 455
DpnII GATC 1 cut(s) 453
EaeI YGGCCR 1 cut(s) 748
Eam1104I CTCTTC 1 cut(s) 710
EarI CTCTTC 1 cut(s) 710
Ecl136II GAGCTC 1 cut(s) 385
Eco130I CCWWGG 1 cut(s) 90
Eco147I AGGCCT 2 cut(s) 400, 466
Eco24I GRGCYC 1 cut(s) 387
Eco47I GGWCC 6 cut(s) 216, 355, 514, 694, 851, 896
Eco53kI GAGCTC 1 cut(s) 385
Eco57I CTGAAG 1 cut(s) 429
EcoICRI GAGCTC 1 cut(s) 385
EcoNI CCTNNNNNAGG 1 cut(s) 837
EcoRI GAATTC 1 cut(s) 525
EcoRII CCWGG 4 cut(s) 301, 358, 520, 574
EcoT14I CCWWGG 1 cut(s) 90
EcoT22I ATGCAT 2 cut(s) 191, 645
EcoT38I GRGCYC 1 cut(s) 387
ErhI CCWWGG 1 cut(s) 90
FaeI CATG 6 cut(s) 223, 355, 643, 811, 889, 999
FaqI GGGAC 3 cut(s) 341, 561, 707
FatI CATG 6 cut(s) 219, 351, 639, 807, 885, 995
FauI CCCGC 1 cut(s) 342
FauNDI CATATG 1 cut(s) 645
Fnu4HI GCNGC 4 cut(s) 6, 555, 558, 651
FriOI GRGCYC 1 cut(s) 387
Fsp4HI GCNGC 4 cut(s) 6, 555, 558, 651
FspBI CTAG 1 cut(s) 380
GlaI GCGC 1 cut(s) 320
GluI GCNGC 4 cut(s) 6, 555, 558, 651
GsuI CTGGAG 1 cut(s) 504
HaeIII GGCC 4 cut(s) 378, 400, 466, 750
HapII CCGG 1 cut(s) 372
HhaI GCGC 1 cut(s) 321
Hin1II CATG 6 cut(s) 223, 355, 643, 811, 889, 999
Hin6I GCGC 1 cut(s) 319
HinP1I GCGC 1 cut(s) 319
HinfI GANTC 5 cut(s) 296, 531, 549, 688, 821
HpaII CCGG 1 cut(s) 372
HphI GGTGA 3 cut(s) 291, 332, 816
Hpy166II GTNNAC 1 cut(s) 225
Hpy188I TCNGA 3 cut(s) 451, 536, 1013
Hpy188III TCNNGA 4 cut(s) 676, 692, 808, 886
Hpy8I GTNNAC 1 cut(s) 225
HpyAV CCTTC 3 cut(s) 317, 404, 623
HpyCH4III ACNGT 1 cut(s) 200
HpyCH4V TGCA 6 cut(s) 189, 272, 557, 643, 740, 1007
HpyF10VI GCNNNNNNNGC 2 cut(s) 28, 33
HpyF3I CTNAG 5 cut(s) 314, 448, 684, 704, 945
Hsp92II CATG 6 cut(s) 223, 355, 643, 811, 889, 999
HspAI GCGC 1 cut(s) 319
Kzo9I GATC 1 cut(s) 453
LmnI GCTCC 2 cut(s) 481, 577
Lsp1109I GCAGC 3 cut(s) 541, 569, 637
LweI GCATC 1 cut(s) 176
MaeI CTAG 1 cut(s) 380
MaeIII GTNAC 2 cut(s) 367, 822
MalI GATC 1 cut(s) 455
MboI GATC 1 cut(s) 453
MboII GAAGA 4 cut(s) 8, 448, 646, 727
MflI RGATCY 1 cut(s) 453
MhlI GDGCHC 2 cut(s) 387, 486
MlsI TGGCCA 1 cut(s) 750
MluCI AATT 7 cut(s) 12, 210, 525, 607, 618, 723, 988
MluNI TGGCCA 1 cut(s) 750
MlyI GAGTC 3 cut(s) 540, 543, 830
MmeI TCCRAC 2 cut(s) 185, 459
Mox20I TGGCCA 1 cut(s) 750
Mph1103I ATGCAT 2 cut(s) 191, 645
MscI TGGCCA 1 cut(s) 750
MseI TTAA 2 cut(s) 150, 603
MslI CAYNNNNRTG 1 cut(s) 644
Msp20I TGGCCA 1 cut(s) 750
MspA1I CMGCKG 1 cut(s) 560
MspI CCGG 1 cut(s) 372
MspR9I CCNGG 4 cut(s) 303, 360, 522, 576
Mva1269I GAATGC 1 cut(s) 1007
MvaI CCWGG 4 cut(s) 303, 360, 522, 576
MvnI CGCG 1 cut(s) 321
MwoI GCNNNNNNNGC 2 cut(s) 28, 33
NdeI CATATG 1 cut(s) 645
NdeII GATC 1 cut(s) 453
NlaIII CATG 6 cut(s) 223, 355, 643, 811, 889, 999
NlaIV GGNNCC 6 cut(s) 217, 291, 357, 516, 695, 771
NmuCI GTSAC 1 cut(s) 822
NsiI ATGCAT 2 cut(s) 191, 645
NspI RCATGY 1 cut(s) 643
NspV TTCGAA 1 cut(s) 120
PagI TCATGA 2 cut(s) 807, 885
PceI AGGCCT 2 cut(s) 400, 466
PctI GAATGC 1 cut(s) 1007
PfeI GAWTC 2 cut(s) 296, 688
PfoI TCCNGGA 1 cut(s) 520
PinAI ACCGGT 1 cut(s) 371
PkrI GCNGC 4 cut(s) 7, 556, 559, 652
PleI GAGTC 3 cut(s) 539, 543, 829
PpsI GAGTC 3 cut(s) 539, 543, 829
Psp124BI GAGCTC 1 cut(s) 387
Psp6I CCWGG 4 cut(s) 301, 358, 520, 574
PspGI CCWGG 4 cut(s) 301, 358, 520, 574
PspN4I GGNNCC 6 cut(s) 217, 291, 357, 516, 695, 771
PspPI GGNCC 6 cut(s) 216, 355, 514, 694, 851, 896
PstI CTGCAG 1 cut(s) 559
PsuI RGATCY 1 cut(s) 453
PvuII CAGCTG 1 cut(s) 560
RsaI GTAC 2 cut(s) 226, 254
RsaNI GTAC 2 cut(s) 225, 253
RseI CAYNNNNRTG 1 cut(s) 644
SacI GAGCTC 1 cut(s) 387
SaqAI TTAA 2 cut(s) 150, 603
SatI GCNGC 4 cut(s) 6, 555, 558, 651
Sau3AI GATC 1 cut(s) 453
Sau96I GGNCC 6 cut(s) 216, 355, 514, 694, 851, 896
SchI GAGTC 3 cut(s) 540, 543, 830
ScrFI CCNGG 4 cut(s) 303, 360, 522, 576
SduI GDGCHC 2 cut(s) 387, 486
SfaNI GCATC 1 cut(s) 176
SfcI CTRYAG 1 cut(s) 555
SfuI TTCGAA 1 cut(s) 120
SinI GGWCC 6 cut(s) 216, 355, 514, 694, 851, 896
SmiMI CAYNNNNRTG 1 cut(s) 644
Sse9I AATT 7 cut(s) 12, 210, 525, 607, 618, 723, 988
SseBI AGGCCT 2 cut(s) 400, 466
SsiI CCGC 1 cut(s) 335
SspMI CTAG 1 cut(s) 380
SstI GAGCTC 1 cut(s) 387
StuI AGGCCT 2 cut(s) 400, 466
StyD4I CCNGG 4 cut(s) 301, 358, 520, 574
StyI CCWWGG 1 cut(s) 90
TaaI ACNGT 1 cut(s) 200
TaqI TCGA 3 cut(s) 120, 529, 819
TaqII GACCGA 1 cut(s) 884
TasI AATT 7 cut(s) 12, 210, 525, 607, 618, 723, 988
TatI WGTACW 2 cut(s) 224, 252
TfiI GAWTC 2 cut(s) 296, 688
Tru1I TTAA 2 cut(s) 150, 603
Tru9I TTAA 2 cut(s) 150, 603
TscAI CASTG 2 cut(s) 498, 688
TseFI GTSAC 1 cut(s) 822
TseI GCWGC 4 cut(s) 5, 554, 557, 650
Tsp45I GTSAC 1 cut(s) 822
TspDTI ATGAA 6 cut(s) 141, 516, 642, 796, 824, 902
TspRI CASTG 2 cut(s) 498, 688
VpaK11BI GGWCC 6 cut(s) 216, 355, 514, 694, 851, 896
XagI CCTNNNNNAGG 1 cut(s) 837
XapI RAATTY 2 cut(s) 525, 607
XceI RCATGY 1 cut(s) 643
XspI CTAG 1 cut(s) 380
Zsp2I ATGCAT 2 cut(s) 191, 645
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.