Rorug05G0295300

Phospholipid diacylglycerol acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0011775
Physical Location & Seq
Reverse (-)
34048424 .. 34050613
2190 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0295300.1

Sequence Viewer

Length: 1833 bp
ATGCTGAAGATGATGAGGGCAACTGCTTCTTCTTCTTGTTATTCTTATTGTTGCAGCAGCACACAACGGAGAAGAAGAGGTATGCCTTTTCTCCTTGACTCTACCACTACTTCTCTTAATGTTTTCCTCAACAGTTTTCATTCTGGGTCCTCAAATCCAAGTAAATCTAGAAACACCCATCTCGTGACAGACCCAGAAACGCCCAAAGTAGTGAGTAATGTTGAGGATGCTTTGGAGGTGTTCCATGAAATGCTTCAGAGGCGTCCTCTGCCTTCCATCATCTGCTTCAATCATATCTTGTGTCAGCTCGTGAAAATGAAGCATTATTCCCGAGTCATTTGTTTGTATAAACAAATGGGTCTGCGGATTATTCCTAATGATTGTAGTCTCAACATTATCATCAACTGCCATTGTCGTTTGAACCAAATGGGATTTAGTTTATCCGTCCTTGGAAAATTCTTCAAATTTGGTCTCCAACCAAATGTCAGGACCTTCAGCACTCTAATCAATGGATTCGTTATGAAGGGTAGAGTCCTTGAAGCAGCTCGGCTTTTCAGTAAAATGGTCGATATAGGTTGTAAACCGGATATTGTTACCTTCGATACTCTAATAAAGGGATTGTGCATCAAAGGCAACAATCGTGCAGCTCTTCAACTGCTTAGGAAGATGGAACTATCTCCGGATTGTAAGCCTGGTGTGTTTTCTTATAGCACCATCATTGATTGTCTTTGTAAGGAGACACTAATTGATGGTGCATTCGACCTCTTCTCGGAAATGATCAGTAAGGGTATTTGCCCAAACGTCGTTACCTATAACACTCTCATCCACGGAGTTTGCCAATTAGGCCAGTGGAAACAAGCTACGAGGTTGTTGAATGAAATGGCGAGTCAAAATATCTATCCGGATGTGCGCACCTTCAATGTGTTGATAGATGCATTTTGCAATGAGGGAATGGTTGTGCAAGCAAAAACCATGGTTGAGATGATGATTCAAAGAGATCTTGAACCTACTGTAGTTACATATAACTCGCTTATGGATGGTTACTGTTTGAGAGGAGAAATGGAGGAGGCAAGAGAAGTGTTCAATCTAATGGTTAGCTTGGGATCAATGGTCGATGCTCAAACTTATGGGATATTGATAAACGGATATTGCAAGCACAAAAATATTAATGAGGCCGTGAGGCTTTTTCAAGAAATGTCTCATAAGGGACTGTTTCCAACTACTGTCACGTACACCACTCTTATGGATGGTTTTTGCAAACTGGGGAGAATACAAGATGCAAAGAAGTTGTTCATTAGGATGCAAGCTTCTGGCCTACTTCCAAATGTTGAGACTTATGGTGTTTTGCTGGATGGCTTTTGTAAAAGGCAACAACTCAGTATGGCAATGGAGTTGCTTAGAGAGATGGAAGGCAAGAAGTTGCATCCAAATATTGTAATTTACAATATTCTTATGGATTATTTTATCAAAGCTGGAAAACTTGAAGCTGCAAGGGATCTATTTTTCAGTTTATCATCAAAAGGACTTCACCCCGATGTTAAGACATATAATGCAATGATTAGTGGATTTTGTAAGGGGGGCTTAATTAGTGAAGCAGAAAATATGCTGAGAGAAATGGAAGAGAAAGGTTGTTCTCCAGATGGTTGTACGTACAACACAATTATCCGAGGGTTTATCCTTAACAATGAGATGTCAAGGGTGATGGCACTTATTCATCAAATGGTGGAGAGGGGTTTCTCAGCAGATGCATCAACAATGAAAATGATAATTGATTTATTGTCTAGAGATAATGTGGATCCAGCTTTGTTGCCCTTGATAAAAGGATCATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 map00561
Pfam Domains
Protein Families
LCAT Lipase_GDSL

Protein Analysis

610

Amino Acids

68.73

Weight (kDa)

8.67

Isoelectric Point (pI)

37.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 125 - 173 3.8e-07 PPR repeat family
PPR_3 PF13812 156 - 205 3e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 157 - 189 1.5e-09 PPR repeat
PPR_2 PF13041 160 - 208 2e-14 PPR repeat family
PPR_3 PF13812 183 - 242 5.4e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 192 - 224 7.3e-11 PPR repeat
PPR_2 PF13041 195 - 245 1.6e-11 PPR repeat family
PPR_1 PF12854 229 - 259 1e-09 PPR repeat
PPR_2 PF13041 231 - 280 4.1e-17 PPR repeat family
PPR PF01535 234 - 264 1.1e-06 PPR repeat
PPR_long PF17177 235 - 345 6.5e-09 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 255 - 315 4.6e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 262 - 294 2.1e-13 PPR repeat
PPR_2 PF13041 266 - 315 4.2e-19 PPR repeat family
PPR PF01535 269 - 298 3.6e-06 PPR repeat
PPR_1 PF12854 298 - 329 3.6e-08 PPR repeat
PPR_2 PF13041 301 - 349 2.4e-13 PPR repeat family
PPR_1 PF12854 333 - 365 2.2e-11 PPR repeat
PPR_2 PF13041 336 - 385 2.3e-16 PPR repeat family
PPR PF01535 339 - 368 3.7e-09 PPR repeat
PPR_long PF17177 354 - 493 3.2e-08 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 369 - 400 6.3e-10 PPR repeat
PPR_2 PF13041 372 - 420 7.3e-18 PPR repeat family
PPR PF01535 375 - 404 3.7e-08 PPR repeat
PPR_1 PF12854 402 - 434 7.7e-12 PPR repeat
PPR_2 PF13041 406 - 455 9.7e-17 PPR repeat family
PPR PF01535 409 - 439 1.7e-08 PPR repeat
PPR_3 PF13812 430 - 488 3e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 438 - 470 3.4e-08 PPR repeat
PPR_2 PF13041 476 - 525 6.2e-16 PPR repeat family
PPR_3 PF13812 504 - 556 8.7e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 507 - 540 5.5e-13 PPR repeat
PPR_2 PF13041 511 - 558 2.2e-16 PPR repeat family
PPR PF01535 515 - 544 7.6e-10 PPR repeat
PPR_2 PF13041 549 - 593 5.8e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011775)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G44830
fragaria_vesca FvH4_3g30050
malus_domestica MD11G1154200.v1.1
prunus_persica Prupe.6G116700_v2.0.a1 Prupe.6G116700_v2.0.a1
pyrus_communis pycom11g12590
rosa_chinensis RchiOBHm_Chr5g0055851 RchiOBHm_Chr5g0055861
rosa_laevigata RLG00000035014
rosa_multiflora Rmu_sc0003306.1_g000008
rosa_roxburghii Rroxscaffold_1G00024510
rosa_rugosa Rorug05G0295200 Rorug05G0295200 Rorug05G0295300
rosa_samantha Rh5AG366600 Rh5BG376800 Rh5CG399800 Rh5DG389800
rosa_wichuraiana Rw5G034360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 911
AccIII TCCGGA 2 cut(s) 679, 901
AciI CCGC 1 cut(s) 364
AclWI GGATC 5 cut(s) 1111, 1503, 1790, 1803, 1831
AcsI RAATTY 2 cut(s) 455, 464
AcuI CTGAAG 3 cut(s) 26, 239, 478
AcyI GRCGYC 1 cut(s) 262
AfaI GTAC 3 cut(s) 1232, 1648, 1652
AfiI CCNNNNNNNGG 1 cut(s) 769
AjnI CCWGG 1 cut(s) 691
AluBI AGCT 9 cut(s) 307, 545, 647, 860, 1098, 1307, 1472, 1487, 1802
AluI AGCT 9 cut(s) 307, 545, 647, 860, 1098, 1307, 1472, 1487, 1802
Alw26I GTCTC 5 cut(s) 392, 476, 731, 1203, 1325
AlwI GGATC 5 cut(s) 1111, 1503, 1790, 1803, 1831
Ama87I CYCGRG 1 cut(s) 330
Aor13HI TCCGGA 2 cut(s) 679, 901
AoxI GGCC 3 cut(s) 844, 1173, 1312
ApeKI GCWGC 5 cut(s) 54, 57, 542, 644, 1487
ApoI RAATTY 2 cut(s) 455, 464
AseI ATTAAT 1 cut(s) 1167
Asp700I GAANNNNTTC 2 cut(s) 252, 1289
AspLEI GCGC 1 cut(s) 912
AspS9I GGNCC 2 cut(s) 147, 489
AsuHPI GGTGA 2 cut(s) 1520, 1711
AvaI CYCGRG 1 cut(s) 330
AvaII GGWCC 2 cut(s) 147, 489
BamHI GGATCC 1 cut(s) 1795
BauI CACGAG 2 cut(s) 182, 308
BbvI GCAGC 5 cut(s) 66, 69, 554, 656, 1474
BceAI ACGGC 1 cut(s) 1160
BciT130I CCWGG 1 cut(s) 693
BclI TGATCA 1 cut(s) 777
BcoDI GTCTC 5 cut(s) 392, 476, 731, 1203, 1325
BfaI CTAG 2 cut(s) 168, 1782
BfmI CTRYAG 1 cut(s) 1011
BglI GCCNNNNNGGC 1 cut(s) 843
BglII AGATCT 1 cut(s) 997
BisI GCNGC 5 cut(s) 55, 58, 543, 645, 1488
BlsI GCNGC 5 cut(s) 56, 59, 544, 646, 1489
Bme1390I CCNGG 1 cut(s) 693
Bme18I GGWCC 2 cut(s) 147, 489
BmeT110I CYCGRG 1 cut(s) 330
BmgT120I GGNCC 2 cut(s) 147, 489
BmiI GGNNCC 2 cut(s) 148, 1797
BmrFI CCNGG 1 cut(s) 693
BmrI ACTGGG 1 cut(s) 1271
BmsI GCATC 9 cut(s) 217, 633, 922, 1105, 1267, 1290, 1432, 1735, 1757
BmuI ACTGGG 1 cut(s) 1271
BplI GAGNNNNNCTC 2 cut(s) 250, 282
BpmI CTGGAG 1 cut(s) 1620
Bpu10I CCTNAGC 1 cut(s) 659
BsaAI YACGTR 2 cut(s) 1230, 1650
BsaHI GRCGYC 1 cut(s) 262
BsaI GGTCTC 1 cut(s) 476
BsaJI CCNNGG 4 cut(s) 448, 826, 972, 1666
BsaWI WCCGGW 3 cut(s) 583, 679, 901
Bsc4I CCNNNNNNNGG 1 cut(s) 769
Bse1I ACTGG 2 cut(s) 847, 1266
Bse3DI GCAATG 3 cut(s) 949, 1392, 1560
BseAI TCCGGA 2 cut(s) 679, 901
BseBI CCWGG 1 cut(s) 693
BseDI CCNNGG 4 cut(s) 448, 826, 972, 1666
BseGI GGATG 8 cut(s) 232, 822, 910, 1042, 1252, 1305, 1357, 1423
BseLI CCNNNNNNNGG 1 cut(s) 769
BseMI GCAATG 3 cut(s) 949, 1392, 1560
BseMII CTCAG 3 cut(s) 1390, 1598, 1752
BseNI ACTGG 2 cut(s) 847, 1266
BseRI GAGGAG 2 cut(s) 1068, 1079
BseXI GCAGC 5 cut(s) 66, 69, 554, 656, 1474
BsgI GTGCAG 1 cut(s) 663
BshFI GGCC 3 cut(s) 846, 1175, 1314
BsiHKCI CYCGRG 1 cut(s) 330
BsiSI CCGG 3 cut(s) 584, 680, 902
BslFI GGGAC 1 cut(s) 1221
BslI CCNNNNNNNGG 1 cut(s) 769
BsmAI GTCTC 5 cut(s) 392, 476, 731, 1203, 1325
BsmFI GGGAC 1 cut(s) 1221
BsmI GAATGC 1 cut(s) 755
BsnI GGCC 3 cut(s) 846, 1175, 1314
Bso31I GGTCTC 1 cut(s) 476
BsoBI CYCGRG 1 cut(s) 330
Bsp13I TCCGGA 2 cut(s) 679, 901
Bsp143I GATC 6 cut(s) 777, 997, 1103, 1495, 1795, 1823
Bsp19I CCATGG 1 cut(s) 972
BspACI CCGC 1 cut(s) 364
BspANI GGCC 3 cut(s) 846, 1175, 1314
BspCNI CTCAG 3 cut(s) 1389, 1599, 1751
BspEI TCCGGA 2 cut(s) 679, 901
BspLI GGNNCC 2 cut(s) 148, 1797
BspPI GGATC 5 cut(s) 1111, 1503, 1790, 1803, 1831
BspQI GCTCTTC 1 cut(s) 654
BspTNI GGTCTC 1 cut(s) 476
BsrDI GCAATG 3 cut(s) 949, 1392, 1560
BsrI ACTGG 2 cut(s) 847, 1266
BssECI CCNNGG 4 cut(s) 448, 826, 972, 1666
BssMI GATC 6 cut(s) 777, 997, 1103, 1495, 1795, 1823
BssNI GRCGYC 1 cut(s) 262
BssSI CACGAG 2 cut(s) 182, 308
BssT1I CCWWGG 2 cut(s) 448, 972
Bst2BI CACGAG 2 cut(s) 182, 308
Bst2UI CCWGG 1 cut(s) 693
Bst4CI ACNGT 5 cut(s) 134, 1012, 1046, 1212, 1225
Bst6I CTCTTC 4 cut(s) 70, 654, 770, 1614
BstACI GRCGYC 1 cut(s) 262
BstBAI YACGTR 2 cut(s) 1230, 1650
BstC8I GCNNGC 3 cut(s) 963, 1154, 1305
BstDEI CTNAG 5 cut(s) 659, 1376, 1397, 1607, 1738
BstDSI CCRYGG 2 cut(s) 826, 972
BstF5I GGATG 8 cut(s) 232, 822, 910, 1042, 1252, 1305, 1357, 1423
BstHHI GCGC 1 cut(s) 912
BstKTI GATC 6 cut(s) 780, 1000, 1106, 1498, 1798, 1826
BstMAI GTCTC 5 cut(s) 392, 476, 731, 1203, 1325
BstMBI GATC 6 cut(s) 777, 997, 1103, 1495, 1795, 1823
BstMWI GCNNNNNNNGC 4 cut(s) 259, 268, 630, 843
BstNI CCWGG 1 cut(s) 693
BstSCI CCNGG 1 cut(s) 691
BstSFI CTRYAG 1 cut(s) 1011
BstSNI TACGTA 1 cut(s) 1650
BstV1I GCAGC 5 cut(s) 66, 69, 554, 656, 1474
BstX2I RGATCY 3 cut(s) 997, 1495, 1795
BstXI CCANNNNNNTGG 1 cut(s) 1243
BstYI RGATCY 3 cut(s) 997, 1495, 1795
BsuRI GGCC 3 cut(s) 846, 1175, 1314
BtgI CCRYGG 2 cut(s) 826, 972
BtsCI GGATG 8 cut(s) 232, 822, 910, 1042, 1252, 1305, 1357, 1423
BtsIMutI CAGTG 1 cut(s) 854
Cac8I GCNNGC 3 cut(s) 963, 1154, 1305
CfoI GCGC 1 cut(s) 912
Cfr13I GGNCC 2 cut(s) 147, 489
CseI GACGC 1 cut(s) 251
Csp6I GTAC 3 cut(s) 1231, 1647, 1651
CspCI CAANNNNNGTGG 2 cut(s) 815, 850
CviAII CATG 2 cut(s) 245, 973
CviQI GTAC 3 cut(s) 1231, 1647, 1651
DdeI CTNAG 5 cut(s) 659, 1376, 1397, 1607, 1738
DpnI GATC 6 cut(s) 779, 999, 1105, 1497, 1797, 1825
DpnII GATC 6 cut(s) 777, 997, 1103, 1495, 1795, 1823
Eam1104I CTCTTC 4 cut(s) 70, 654, 770, 1614
EarI CTCTTC 4 cut(s) 70, 654, 770, 1614
Eco105I TACGTA 1 cut(s) 1650
Eco130I CCWWGG 2 cut(s) 448, 972
Eco31I GGTCTC 1 cut(s) 476
Eco47I GGWCC 2 cut(s) 147, 489
Eco57I CTGAAG 3 cut(s) 26, 239, 478
Eco88I CYCGRG 1 cut(s) 330
EcoO109I RGGNCCY 2 cut(s) 147, 489
EcoRII CCWGG 1 cut(s) 691
EcoT14I CCWWGG 2 cut(s) 448, 972
EcoT22I ATGCAT 2 cut(s) 937, 1750
ErhI CCWWGG 2 cut(s) 448, 972
FaeI CATG 2 cut(s) 248, 976
FalI AAGNNNNNCTT 2 cut(s) 1565, 1597
FaqI GGGAC 1 cut(s) 1221
FatI CATG 2 cut(s) 244, 972
FbaI TGATCA 1 cut(s) 777
Fnu4HI GCNGC 5 cut(s) 55, 58, 543, 645, 1488
FokI GGATG 8 cut(s) 239, 809, 917, 1049, 1259, 1312, 1364, 1410
Fsp4HI GCNGC 5 cut(s) 55, 58, 543, 645, 1488
FspAI RTGCGCAY 1 cut(s) 911
FspBI CTAG 2 cut(s) 168, 1782
FspI TGCGCA 1 cut(s) 911
GlaI GCGC 1 cut(s) 911
GluI GCNGC 5 cut(s) 55, 58, 543, 645, 1488
GsuI CTGGAG 1 cut(s) 1620
HaeIII GGCC 3 cut(s) 846, 1175, 1314
HapII CCGG 3 cut(s) 584, 680, 902
HgaI GACGC 1 cut(s) 251
HhaI GCGC 1 cut(s) 912
Hin1I GRCGYC 1 cut(s) 262
Hin1II CATG 2 cut(s) 248, 976
Hin6I GCGC 1 cut(s) 910
HinP1I GCGC 1 cut(s) 910
HindIII AAGCTT 1 cut(s) 1305
HinfI GANTC 6 cut(s) 98, 333, 513, 531, 886, 988
HpaII CCGG 3 cut(s) 584, 680, 902
HphI GGTGA 2 cut(s) 1520, 1711
Hpy166II GTNNAC 2 cut(s) 581, 1233
Hpy188I TCNGA 3 cut(s) 258, 772, 1667
Hpy8I GTNNAC 2 cut(s) 581, 1233
Hpy99I CGWCG 1 cut(s) 806
HpyAV CCTTC 6 cut(s) 282, 502, 517, 607, 925, 1403
HpyCH4III ACNGT 5 cut(s) 134, 1012, 1046, 1212, 1225
HpyCH4IV ACGT 3 cut(s) 801, 1229, 1649
HpyF10VI GCNNNNNNNGC 4 cut(s) 259, 268, 630, 843
HpyF3I CTNAG 5 cut(s) 659, 1376, 1397, 1607, 1738
HpySE526I ACGT 3 cut(s) 801, 1229, 1649
Hsp92I GRCGYC 1 cut(s) 262
Hsp92II CATG 2 cut(s) 248, 976
HspAI GCGC 1 cut(s) 910
Kpn2I TCCGGA 2 cut(s) 679, 901
Ksp22I TGATCA 1 cut(s) 777
Kzo9I GATC 6 cut(s) 777, 997, 1103, 1495, 1795, 1823
LguI GCTCTTC 1 cut(s) 654
Lsp1109I GCAGC 5 cut(s) 66, 69, 554, 656, 1474
LweI GCATC 9 cut(s) 217, 633, 922, 1105, 1267, 1290, 1432, 1735, 1757
MaeI CTAG 2 cut(s) 168, 1782
MaeII ACGT 3 cut(s) 801, 1229, 1649
MaeIII GTNAC 6 cut(s) 184, 592, 805, 1015, 1040, 1225
MalI GATC 6 cut(s) 779, 999, 1105, 1497, 1797, 1825
MboI GATC 6 cut(s) 777, 997, 1103, 1495, 1795, 1823
MflI RGATCY 3 cut(s) 997, 1495, 1795
MluCI AATT 8 cut(s) 455, 464, 744, 839, 1437, 1584, 1659, 1767
MlyI GAGTC 4 cut(s) 92, 342, 540, 895
MmeI TCCRAC 2 cut(s) 499, 1241
Mph1103I ATGCAT 2 cut(s) 937, 1750
MroI TCCGGA 2 cut(s) 679, 901
MroXI GAANNNNTTC 2 cut(s) 252, 1289
MseI TTAA 5 cut(s) 117, 1167, 1539, 1583, 1680
MslI CAYNNNNRTG 3 cut(s) 1241, 1298, 1533
MspI CCGG 3 cut(s) 584, 680, 902
MspR9I CCNGG 1 cut(s) 693
Mva1269I GAATGC 1 cut(s) 755
MvaI CCWGG 1 cut(s) 693
MwoI GCNNNNNNNGC 4 cut(s) 259, 268, 630, 843
NcoI CCATGG 1 cut(s) 972
NdeII GATC 6 cut(s) 777, 997, 1103, 1495, 1795, 1823
NlaIII CATG 2 cut(s) 248, 976
NlaIV GGNNCC 2 cut(s) 148, 1797
NmeAIII GCCGAG 1 cut(s) 526
NmuCI GTSAC 2 cut(s) 184, 1225
NsbI TGCGCA 1 cut(s) 911
NsiI ATGCAT 2 cut(s) 937, 1750
PciSI GCTCTTC 1 cut(s) 654
PctI GAATGC 1 cut(s) 755
PdmI GAANNNNTTC 2 cut(s) 252, 1289
PfeI GAWTC 2 cut(s) 513, 988
PkrI GCNGC 5 cut(s) 56, 59, 544, 646, 1489
PleI GAGTC 4 cut(s) 92, 341, 539, 894
PpsI GAGTC 4 cut(s) 92, 341, 539, 894
Ppu21I YACGTR 2 cut(s) 1230, 1650
PpuMI RGGWCCY 2 cut(s) 147, 489
PshBI ATTAAT 1 cut(s) 1167
Psp5II RGGWCCY 2 cut(s) 147, 489
Psp6I CCWGG 1 cut(s) 691
PspGI CCWGG 1 cut(s) 691
PspN4I GGNNCC 2 cut(s) 148, 1797
PspPI GGNCC 2 cut(s) 147, 489
PspPPI RGGWCCY 2 cut(s) 147, 489
PsuI RGATCY 3 cut(s) 997, 1495, 1795
RsaI GTAC 3 cut(s) 1232, 1648, 1652
RsaNI GTAC 3 cut(s) 1231, 1647, 1651
RseI CAYNNNNRTG 3 cut(s) 1241, 1298, 1533
SapI GCTCTTC 1 cut(s) 654
SaqAI TTAA 5 cut(s) 117, 1167, 1539, 1583, 1680
SatI GCNGC 5 cut(s) 55, 58, 543, 645, 1488
Sau3AI GATC 6 cut(s) 777, 997, 1103, 1495, 1795, 1823
Sau96I GGNCC 2 cut(s) 147, 489
SchI GAGTC 4 cut(s) 92, 342, 540, 895
ScrFI CCNGG 1 cut(s) 693
SfaNI GCATC 9 cut(s) 217, 633, 922, 1105, 1267, 1290, 1432, 1735, 1757
SfcI CTRYAG 1 cut(s) 1011
SinI GGWCC 2 cut(s) 147, 489
SmiMI CAYNNNNRTG 3 cut(s) 1241, 1298, 1533
SnaBI TACGTA 1 cut(s) 1650
Sse9I AATT 8 cut(s) 455, 464, 744, 839, 1437, 1584, 1659, 1767
SsiI CCGC 1 cut(s) 364
SspI AATATT 3 cut(s) 1165, 1432, 1447
SspMI CTAG 2 cut(s) 168, 1782
StyD4I CCNGG 1 cut(s) 691
StyI CCWWGG 2 cut(s) 448, 972
TaaI ACNGT 5 cut(s) 134, 1012, 1046, 1212, 1225
TaiI ACGT 3 cut(s) 804, 1232, 1652
TaqI TCGA 4 cut(s) 567, 600, 759, 1113
TasI AATT 8 cut(s) 455, 464, 744, 839, 1437, 1584, 1659, 1767
TfiI GAWTC 2 cut(s) 513, 988
Tru1I TTAA 5 cut(s) 117, 1167, 1539, 1583, 1680
Tru9I TTAA 5 cut(s) 117, 1167, 1539, 1583, 1680
TscAI CASTG 1 cut(s) 854
TseFI GTSAC 2 cut(s) 184, 1225
TseI GCWGC 5 cut(s) 54, 57, 542, 644, 1487
Tsp45I GTSAC 2 cut(s) 184, 1225
TspDTI ATGAA 8 cut(s) 128, 261, 332, 536, 891, 1282, 1703, 1772
TspGWI ACGGA 4 cut(s) 82, 433, 843, 1158
TspRI CASTG 1 cut(s) 854
VpaK11BI GGWCC 2 cut(s) 147, 489
VspI ATTAAT 1 cut(s) 1167
XapI RAATTY 2 cut(s) 455, 464
XbaI TCTAGA 2 cut(s) 167, 1781
XmnI GAANNNNTTC 2 cut(s) 252, 1289
XspI CTAG 2 cut(s) 168, 1782
Zsp2I ATGCAT 2 cut(s) 937, 1750
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.