pycom11g12590

Phospholipid diacylglycerol acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0011775
Physical Location & Seq
Reverse (-)
11486884 .. 11490791
3908 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g12590.1

Sequence Viewer

Length: 2043 bp
ATGGCTTCGAGATCGATTCTCCGGTTTCGAAAACTCTGCTATGTTGAGCCTGTGAGCTCCCCTTCTGTAAAGTTTCAATCCTTTCAGACCCCAGAAGTTGAAAAGACAGATGATAGTTCCGTGGAGAAGAAGCAATGGAAGAGGAAGAAGCAGCAGAGGGGGTGGAGGTGCATAGATAGCTGCTGTTGGGTCATTGGGTACATGTGCACTGCTTGGTGGGTTTTGATGTTATTGTACCACAGCTTGCCGGTCACTTTGACCGGCTTTCAGGTGCCGGAATCTCCAGGGACAAGGCTTAAAGCTGAAGGCCTGACTGCCCTTCACCCTGTTGTTCTTGTCCCCGGCATTGTGACCGGTGGCCTCGAGCTCTGGGAAGGCAGGCCTTGTGCCGAGGGGCTTTTTCGAAAGCGGCTTTGGGGTGGTGGTTTCACTGAAATCTTCAAAAGGCCTTTGTGTTGGTTGGAGCATCTGTCTCTGGACAATGAAACAGGGCTCGATCCTCCGGGAATTCGAGTCAGGGCAGTTCCGGGACTTGTTGCAGCTGACTATTTTGCTCCTGGATATTTTGTTTGGGCTGTTTTGATCGAAAACTTGGCGAGAATGGGTTACGAAGGGAAGAATATGCATATGGCCGCGTATGATTGGCGGCTATCTTTCCAAAATACAGAGATTCGGGACCAAGCTCTTAGTAGATTGAAGAGTAAAATTGAGCTTATGTACGTAACGAATGGATATAAGAAAGTGGTTGTGGTGCCTCATTCTATGGGTGCAATTTATTTTCTCCACTTCATGAAATGGGTTGAAACACCTCCTCCTAAAGGTGGTGGTGGGGGTCCAAGCTGGTGTGCCAAGCACATTAAAGCAATCATGAATATCGGTCCAGCATTTCTTGGGGTTCCAAAAGCTGTTAGTAATATATTTTCTGCTGAGGGTAAAGATGTCGCGTACATCAGAGCTATGGCTCCGGGTGTTTTAGATTCTGAATTTCTTGGGCTTCAAACCTTTGAGCACGTCATGAGGGCATGTCGAACCTGGGATTCCATAGTTTCCATATTGCCAAAAGGTGGAGATGCCATCTGGGGCAATTTGGATTGGTCTCCTGAAGATGGGCAGAGCTGTGACCGGGCAAAGAAAGGATATCTACAGTCGACTTCCGGTGACAACAATTTCAGCCACAACGATGGAAATAGAATTTTCCAAGTAAAAGAACCTGTGAAATATGGAAGAATAATCTCTTTTGGCAAGGAATCTTCACAATTATCTTCTTCACAGCTTCCTAGGCTTGATTTGAAGGATCTAATACACAAAGATTCGGTCACAAATTCCAAGTCATCATGTGGAGGGGTGTGGACTGAATATGGTGAGGTAAGCAGTGAAAGCATCAGAAGAGTTACAGAAAATAAGGCTTACACAGCTCAAACACTTTTTGATCTACTACGTTTTGTGGCTCCAAAAATGATGCGACGGGCTGAGGCTCACTTCTCTCATGGGATAGCTGATAACCTTGATGATCCTAAATACGCCCATTACAAGTACTGGTCCAATCCACTAGAGACCAAGTTACCGGTTGCTCCAGACATGGAAATATACTGTTTATATGGCGTTGGAATCCCCACTGAAAGGTCATATATATACAAGATGTCTCCTCCTAACAAGTGCAAGAGCATTCCATTCCGGATCGATGGCTCAGCAGATGGAGAGGCAGGAAGTTGTCTGAAAAATGGAATACATTTTGTGGATGGTGATGATAGTGTGCCTGTATTGAGTGCAGGATTCATGTGTGCCAAAGGATGGAGAGGAAGAACAAGATTCAACCCATCTGGTATTGCTACCTACATAAGGGAGTACCGGCACAAGCCACCGGCTAGTCTCCTTGAGGGGAGGGGTATAGAGAGTGGTGCACATGTTGACATCATGGGAAACGTTGCATTAATCGAAGATGTTCTCAGGGTTGCTGCCGGAGCAAATGGTGCGCAGACCGGAGGGGATAGGATTTACTCAGATATCATGAGAATTTCTGAGAGAATAAATCTACAGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 map00561
Pfam Domains
Protein Families
LCAT Lipase_GDSL

Protein Analysis

681

Amino Acids

75.87

Weight (kDa)

8.87

Isoelectric Point (pI)

49.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCAT PF02450 134 - 640 4.3e-64 Lecithin:cholesterol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011775)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G44830
fragaria_vesca FvH4_3g30050
malus_domestica MD11G1154200.v1.1
prunus_persica Prupe.6G116700_v2.0.a1 Prupe.6G116700_v2.0.a1
pyrus_communis pycom11g12590
rosa_chinensis RchiOBHm_Chr5g0055851 RchiOBHm_Chr5g0055861
rosa_laevigata RLG00000035014
rosa_multiflora Rmu_sc0003306.1_g000008
rosa_roxburghii Rroxscaffold_1G00024510
rosa_rugosa Rorug05G0295200 Rorug05G0295200 Rorug05G0295300
rosa_samantha Rh5AG366600 Rh5BG376800 Rh5CG399800 Rh5DG389800
rosa_wichuraiana Rw5G034360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1974
AccB1I GGYRCC 2 cut(s) 271, 751
AccB7I CCANNNNNTGG 2 cut(s) 1064, 1791
AccI GTMKAC 1 cut(s) 1148
AccII CGCG 2 cut(s) 635, 944
AccIII TCCGGA 1 cut(s) 1674
AciI CCGC 3 cut(s) 409, 633, 646
AclI AACGTT 1 cut(s) 1923
AclWI GGATC 4 cut(s) 491, 1302, 1505, 1685
AcoI YGGCCR 1 cut(s) 630
AcsI RAATTY 5 cut(s) 507, 983, 1191, 1321, 2013
AcuI CTGAAG 2 cut(s) 324, 1122
AfaI GTAC 6 cut(s) 200, 236, 719, 947, 1535, 1847
AfiI CCNNNNNNNGG 7 cut(s) 818, 821, 1064, 1106, 1620, 1791, 1839
AflIII ACRYGT 2 cut(s) 201, 1903
AgeI ACCGGT 2 cut(s) 353, 1564
AgsI TTSAA 8 cut(s) 77, 101, 442, 697, 803, 998, 1291, 1813
AjiI CACGTC 1 cut(s) 1012
AjnI CCWGG 3 cut(s) 283, 556, 1031
AjuI GAANNNNNNNTTGG 2 cut(s) 397, 429
Alw21I GWGCWC 5 cut(s) 59, 209, 369, 1011, 1903
Alw26I GTCTC 5 cut(s) 477, 1101, 1547, 1647, 1874
Alw44I GTGCAC 2 cut(s) 205, 1899
AlwI GGATC 4 cut(s) 491, 1302, 1505, 1685
Ama87I CYCGRG 1 cut(s) 362
Aor13HI TCCGGA 1 cut(s) 1674
AoxI GGCC 5 cut(s) 307, 358, 380, 446, 630
ApaLI GTGCAC 2 cut(s) 205, 1899
ApeKI GCWGC 4 cut(s) 151, 180, 539, 1955
ApoI RAATTY 5 cut(s) 507, 983, 1191, 1321, 2013
ArsI GACNNNNNNTTYG 4 cut(s) 1299, 1313, 1331, 1345
AseI ATTAAT 1 cut(s) 1931
AsiGI ACCGGT 2 cut(s) 353, 1564
Asp700I GAANNNNTTC 1 cut(s) 1941
AspA2I CCTAGG 1 cut(s) 1277
AspLEI GCGC 1 cut(s) 1975
AspS9I GGNCC 4 cut(s) 676, 833, 878, 1539
AsuC2I CCSGG 5 cut(s) 342, 504, 528, 966, 1124
AsuHPI GGTGA 4 cut(s) 314, 1169, 1373, 1754
AsuII TTCGAA 2 cut(s) 28, 403
AvaI CYCGRG 1 cut(s) 362
AvaII GGWCC 4 cut(s) 676, 833, 878, 1539
AvrII CCTAGG 1 cut(s) 1277
BaeGI GKGCMC 2 cut(s) 209, 1903
BanI GGYRCC 2 cut(s) 271, 751
BanII GRGCYC 3 cut(s) 59, 369, 495
Bbv12I GWGCWC 5 cut(s) 59, 209, 369, 1011, 1903
BbvCI CCTCAGC 2 cut(s) 927, 1470
BbvI GCAGC 4 cut(s) 163, 167, 551, 1942
BccI CCATC 8 cut(s) 1082, 1100, 1175, 1676, 1688, 1733, 1785, 1825
BcgI CGANNNNNNTGC 2 cut(s) 18, 52
BciT130I CCWGG 3 cut(s) 285, 558, 1033
BcnI CCSGG 5 cut(s) 342, 504, 528, 966, 1124
BcoDI GTCTC 5 cut(s) 477, 1101, 1547, 1647, 1874
BfaI CTAG 3 cut(s) 1278, 1550, 1866
BfmI CTRYAG 2 cut(s) 1142, 2033
BisI GCNGC 7 cut(s) 152, 181, 410, 540, 633, 647, 1956
BlnI CCTAGG 1 cut(s) 1277
BlpI GCTNAGC 1 cut(s) 1687
BlsI GCNGC 7 cut(s) 153, 182, 411, 541, 634, 648, 1957
BmcAI AGTACT 1 cut(s) 1535
Bme1390I CCNGG 8 cut(s) 285, 342, 504, 528, 558, 966, 1033, 1124
Bme18I GGWCC 4 cut(s) 676, 833, 878, 1539
BmeT110I CYCGRG 1 cut(s) 362
BmgBI CACGTC 1 cut(s) 1012
BmgT120I GGNCC 4 cut(s) 676, 833, 878, 1539
BmiI GGNNCC 7 cut(s) 273, 677, 753, 834, 897, 963, 1449
BmrFI CCNGG 8 cut(s) 285, 342, 504, 528, 558, 966, 1033, 1124
BmsI GCATC 4 cut(s) 475, 1060, 1389, 1449
BpmI CTGGAG 2 cut(s) 267, 1557
Bpu10I CCTNAGC 2 cut(s) 927, 1470
Bpu1102I GCTNAGC 1 cut(s) 1687
Bpu14I TTCGAA 2 cut(s) 28, 403
BpuEI CTTGAG 1 cut(s) 1895
BpuMI CCSGG 5 cut(s) 342, 504, 528, 966, 1124
Bsa29I ATCGAT 2 cut(s) 14, 1680
BsaAI YACGTR 1 cut(s) 721
BsaI GGTCTC 2 cut(s) 1101, 1547
BsaJI CCNNGG 6 cut(s) 120, 284, 340, 390, 1032, 1277
BsaWI WCCGGW 6 cut(s) 21, 353, 1154, 1564, 1674, 1979
BsaXI ACNNNNNCTCC 4 cut(s) 455, 485, 796, 826
Bsc4I CCNNNNNNNGG 7 cut(s) 818, 821, 1064, 1106, 1620, 1791, 1839
Bse118I RCCGGY 6 cut(s) 247, 260, 353, 1564, 1848, 1861
Bse1I ACTGG 1 cut(s) 1541
Bse3DI GCAATG 1 cut(s) 140
BseAI TCCGGA 1 cut(s) 1674
BseBI CCWGG 3 cut(s) 285, 558, 1033
BseCI ATCGAT 2 cut(s) 14, 1680
BseDI CCNNGG 6 cut(s) 120, 284, 340, 390, 1032, 1277
BseGI GGATG 2 cut(s) 1744, 1796
BseLI CCNNNNNNNGG 7 cut(s) 818, 821, 1064, 1106, 1620, 1791, 1839
BseMI GCAATG 1 cut(s) 140
BseMII CTCAG 6 cut(s) 918, 1461, 1701, 1960, 2010, 2013
BseNI ACTGG 1 cut(s) 1541
BseRI GAGGAG 2 cut(s) 801, 1635
BseSI GKGCMC 2 cut(s) 209, 1903
BseXI GCAGC 4 cut(s) 163, 167, 551, 1942
BsgI GTGCAG 1 cut(s) 1788
Bsh1236I CGCG 2 cut(s) 635, 944
BshFI GGCC 5 cut(s) 309, 360, 382, 448, 632
BshNI GGYRCC 2 cut(s) 271, 751
BshTI ACCGGT 2 cut(s) 353, 1564
BshVI ATCGAT 2 cut(s) 14, 1680
BsiHKAI GWGCWC 5 cut(s) 59, 209, 369, 1011, 1903
BsiHKCI CYCGRG 1 cut(s) 362
BslFI GGGAC 4 cut(s) 301, 323, 543, 689
BslI CCNNNNNNNGG 7 cut(s) 818, 821, 1064, 1106, 1620, 1791, 1839
BsmAI GTCTC 5 cut(s) 477, 1101, 1547, 1647, 1874
BsmFI GGGAC 4 cut(s) 301, 323, 543, 689
BsmI GAATGC 1 cut(s) 1665
BsnI GGCC 5 cut(s) 309, 360, 382, 448, 632
Bso31I GGTCTC 2 cut(s) 1101, 1547
BsoBI CYCGRG 1 cut(s) 362
Bsp119I TTCGAA 2 cut(s) 28, 403
Bsp1286I GDGCHC 6 cut(s) 59, 209, 369, 495, 1011, 1903
Bsp13I TCCGGA 1 cut(s) 1674
Bsp143I GATC 7 cut(s) 11, 496, 582, 1294, 1429, 1510, 1677
Bsp1720I GCTNAGC 1 cut(s) 1687
BspACI CCGC 3 cut(s) 409, 633, 646
BspANI GGCC 5 cut(s) 309, 360, 382, 448, 632
BspCNI CTCAG 6 cut(s) 919, 1462, 1700, 1959, 2011, 2012
BspDI ATCGAT 2 cut(s) 14, 1680
BspEI TCCGGA 1 cut(s) 1674
BspFNI CGCG 2 cut(s) 635, 944
BspHI TCATGA 4 cut(s) 789, 867, 1014, 2007
BspLI GGNNCC 7 cut(s) 273, 677, 753, 834, 897, 963, 1449
BspPI GGATC 4 cut(s) 491, 1302, 1505, 1685
BspT104I TTCGAA 2 cut(s) 28, 403
BspT107I GGYRCC 2 cut(s) 271, 751
BspTNI GGTCTC 2 cut(s) 1101, 1547
BsrDI GCAATG 1 cut(s) 140
BsrFI RCCGGY 6 cut(s) 247, 260, 353, 1564, 1848, 1861
BsrI ACTGG 1 cut(s) 1541
BssAI RCCGGY 6 cut(s) 247, 260, 353, 1564, 1848, 1861
BssECI CCNNGG 6 cut(s) 120, 284, 340, 390, 1032, 1277
BssMI GATC 7 cut(s) 11, 496, 582, 1294, 1429, 1510, 1677
BssT1I CCWWGG 1 cut(s) 1277
Bst2UI CCWGG 3 cut(s) 285, 558, 1033
Bst4CI ACNGT 2 cut(s) 1146, 1592
Bst6I CTCTTC 3 cut(s) 134, 692, 1381
BstAPI GCANNNNNTGC 1 cut(s) 1970
BstBAI YACGTR 1 cut(s) 721
BstBI TTCGAA 2 cut(s) 28, 403
BstC8I GCNNGC 2 cut(s) 245, 380
BstDEI CTNAG 7 cut(s) 686, 927, 1470, 1687, 1946, 1999, 2019
BstDSI CCRYGG 1 cut(s) 120
BstENI CCTNNNNNAGG 2 cut(s) 816, 1837
BstF5I GGATG 2 cut(s) 1744, 1796
BstFNI CGCG 2 cut(s) 635, 944
BstHHI GCGC 1 cut(s) 1975
BstKTI GATC 7 cut(s) 14, 499, 585, 1297, 1432, 1513, 1680
BstMAI GTCTC 5 cut(s) 477, 1101, 1547, 1647, 1874
BstMBI GATC 7 cut(s) 11, 496, 582, 1294, 1429, 1510, 1677
BstMWI GCNNNNNNNGC 6 cut(s) 177, 1279, 1377, 1412, 1961, 1970
BstNI CCWGG 3 cut(s) 285, 558, 1033
BstNSI RCATGY 3 cut(s) 205, 1026, 1907
BstSCI CCNGG 8 cut(s) 283, 340, 502, 526, 556, 964, 1031, 1122
BstSFI CTRYAG 2 cut(s) 1142, 2033
BstSLI GKGCMC 2 cut(s) 209, 1903
BstSNI TACGTA 1 cut(s) 721
BstUI CGCG 2 cut(s) 635, 944
BstV1I GCAGC 4 cut(s) 163, 167, 551, 1942
BstX2I RGATCY 1 cut(s) 1294
BstXI CCANNNNNNTGG 1 cut(s) 1181
BstYI RGATCY 1 cut(s) 1294
Bsu15I ATCGAT 2 cut(s) 14, 1680
BsuRI GGCC 5 cut(s) 309, 360, 382, 448, 632
BsuTUI ATCGAT 2 cut(s) 14, 1680
BtgI CCRYGG 1 cut(s) 120
BtrI CACGTC 1 cut(s) 1012
BtsCI GGATG 2 cut(s) 1744, 1796
BtsI GCAGTG 2 cut(s) 207, 1378
BtsIMutI CAGTG 4 cut(s) 207, 429, 1378, 1614
Cac8I GCNNGC 2 cut(s) 245, 380
CciI TCATGA 4 cut(s) 789, 867, 1014, 2007
CfoI GCGC 1 cut(s) 1975
Cfr10I RCCGGY 6 cut(s) 247, 260, 353, 1564, 1848, 1861
Cfr13I GGNCC 4 cut(s) 676, 833, 878, 1539
ClaI ATCGAT 2 cut(s) 14, 1680
Csp6I GTAC 6 cut(s) 199, 235, 718, 946, 1534, 1846
CspAI ACCGGT 2 cut(s) 353, 1564
CviQI GTAC 6 cut(s) 199, 235, 718, 946, 1534, 1846
DdeI CTNAG 7 cut(s) 686, 927, 1470, 1687, 1946, 1999, 2019
DpnI GATC 7 cut(s) 13, 498, 584, 1296, 1431, 1512, 1679
DpnII GATC 7 cut(s) 11, 496, 582, 1294, 1429, 1510, 1677
EaeI YGGCCR 1 cut(s) 630
Eam1104I CTCTTC 3 cut(s) 134, 692, 1381
EarI CTCTTC 3 cut(s) 134, 692, 1381
Ecl136II GAGCTC 2 cut(s) 57, 367
Eco105I TACGTA 1 cut(s) 721
Eco130I CCWWGG 1 cut(s) 1277
Eco147I AGGCCT 3 cut(s) 309, 382, 448
Eco24I GRGCYC 3 cut(s) 59, 369, 495
Eco31I GGTCTC 2 cut(s) 1101, 1547
Eco32I GATATC 2 cut(s) 1139, 2005
Eco47I GGWCC 4 cut(s) 676, 833, 878, 1539
Eco53kI GAGCTC 2 cut(s) 57, 367
Eco57I CTGAAG 2 cut(s) 324, 1122
Eco88I CYCGRG 1 cut(s) 362
EcoICRI GAGCTC 2 cut(s) 57, 367
EcoNI CCTNNNNNAGG 2 cut(s) 816, 1837
EcoRI GAATTC 1 cut(s) 507
EcoRII CCWGG 3 cut(s) 283, 556, 1031
EcoRV GATATC 2 cut(s) 1139, 2005
EcoT14I CCWWGG 1 cut(s) 1277
EcoT22I ATGCAT 1 cut(s) 627
EcoT38I GRGCYC 3 cut(s) 59, 369, 495
ErhI CCWWGG 1 cut(s) 1277
FaqI GGGAC 4 cut(s) 301, 323, 543, 689
FauNDI CATATG 1 cut(s) 627
FblI GTMKAC 1 cut(s) 1148
Fnu4HI GCNGC 7 cut(s) 152, 181, 410, 540, 633, 647, 1956
FokI GGATG 2 cut(s) 1751, 1803
FriOI GRGCYC 3 cut(s) 59, 369, 495
Fsp4HI GCNGC 7 cut(s) 152, 181, 410, 540, 633, 647, 1956
FspBI CTAG 3 cut(s) 1278, 1550, 1866
FspI TGCGCA 1 cut(s) 1974
GlaI GCGC 1 cut(s) 1974
GluI GCNGC 7 cut(s) 152, 181, 410, 540, 633, 647, 1956
GsuI CTGGAG 2 cut(s) 267, 1557
HaeIII GGCC 5 cut(s) 309, 360, 382, 448, 632
HhaI GCGC 1 cut(s) 1975
Hin6I GCGC 1 cut(s) 1973
HinP1I GCGC 1 cut(s) 1973
HincII GTYRAC 2 cut(s) 1149, 1909
HindII GTYRAC 2 cut(s) 1149, 1909
HphI GGTGA 4 cut(s) 314, 1169, 1373, 1754
Hpy166II GTNNAC 5 cut(s) 207, 1149, 1350, 1901, 1909
Hpy188I TCNGA 7 cut(s) 87, 953, 982, 1385, 1716, 2002, 2020
Hpy8I GTNNAC 5 cut(s) 207, 1149, 1350, 1901, 1909
Hpy99I CGWCG 1 cut(s) 1467
HpyAV CCTTC 6 cut(s) 72, 299, 329, 368, 605, 1285
HpyCH4III ACNGT 2 cut(s) 1146, 1592
HpyCH4IV ACGT 4 cut(s) 720, 1011, 1438, 1923
HpyCH4V TGCA 9 cut(s) 171, 207, 539, 625, 770, 1659, 1769, 1901, 1928
HpyF10VI GCNNNNNNNGC 6 cut(s) 177, 1279, 1377, 1412, 1961, 1970
HpyF3I CTNAG 7 cut(s) 686, 927, 1470, 1687, 1946, 1999, 2019
HpySE526I ACGT 4 cut(s) 720, 1011, 1438, 1923
HspAI GCGC 1 cut(s) 1973
Kpn2I TCCGGA 1 cut(s) 1674
Kzo9I GATC 7 cut(s) 11, 496, 582, 1294, 1429, 1510, 1677
LmnI GCTCC 7 cut(s) 62, 463, 559, 967, 1453, 1576, 1961
Lsp1109I GCAGC 4 cut(s) 163, 167, 551, 1942
LweI GCATC 4 cut(s) 475, 1060, 1389, 1449
MaeI CTAG 3 cut(s) 1278, 1550, 1866
MaeII ACGT 4 cut(s) 720, 1011, 1438, 1923
MaeIII GTNAC 9 cut(s) 250, 349, 605, 721, 1118, 1157, 1315, 1390, 1560
MalI GATC 7 cut(s) 13, 498, 584, 1296, 1431, 1512, 1679
MboI GATC 7 cut(s) 11, 496, 582, 1294, 1429, 1510, 1677
MflI RGATCY 1 cut(s) 1294
MhlI GDGCHC 6 cut(s) 59, 209, 369, 495, 1011, 1903
MlyI GAGTC 1 cut(s) 522
MmeI TCCRAC 2 cut(s) 441, 1585
Mph1103I ATGCAT 1 cut(s) 627
MroI TCCGGA 1 cut(s) 1674
MroXI GAANNNNTTC 1 cut(s) 1941
MseI TTAA 3 cut(s) 297, 858, 1931
MslI CAYNNNNRTG 1 cut(s) 1179
MspA1I CMGCKG 1 cut(s) 542
MspR9I CCNGG 8 cut(s) 285, 342, 504, 528, 558, 966, 1033, 1124
Mva1269I GAATGC 1 cut(s) 1665
MvaI CCWGG 3 cut(s) 285, 558, 1033
MvnI CGCG 2 cut(s) 635, 944
MwoI GCNNNNNNNGC 6 cut(s) 177, 1279, 1377, 1412, 1961, 1970
NciI CCSGG 5 cut(s) 342, 504, 528, 966, 1124
NdeI CATATG 1 cut(s) 627
NdeII GATC 7 cut(s) 11, 496, 582, 1294, 1429, 1510, 1677
NlaIV GGNNCC 7 cut(s) 273, 677, 753, 834, 897, 963, 1449
NmeAIII GCCGAG 1 cut(s) 415
NmuCI GTSAC 5 cut(s) 250, 349, 1118, 1157, 1315
NsbI TGCGCA 1 cut(s) 1974
NsiI ATGCAT 1 cut(s) 627
NspI RCATGY 3 cut(s) 205, 1026, 1907
NspV TTCGAA 2 cut(s) 28, 403
PaeR7I CTCGAG 1 cut(s) 362
PagI TCATGA 4 cut(s) 789, 867, 1014, 2007
PceI AGGCCT 3 cut(s) 309, 382, 448
PciI ACATGT 2 cut(s) 201, 1903
PctI GAATGC 1 cut(s) 1665
PdmI GAANNNNTTC 1 cut(s) 1941
PflMI CCANNNNNTGG 2 cut(s) 1064, 1791
PfoI TCCNGGA 3 cut(s) 502, 526, 556
PinAI ACCGGT 2 cut(s) 353, 1564
PkrI GCNGC 7 cut(s) 153, 182, 411, 541, 634, 648, 1957
PleI GAGTC 1 cut(s) 521
PpsI GAGTC 1 cut(s) 521
Ppu21I YACGTR 1 cut(s) 721
PscI ACATGT 2 cut(s) 201, 1903
PshBI ATTAAT 1 cut(s) 1931
Psp124BI GAGCTC 2 cut(s) 59, 369
Psp1406I AACGTT 1 cut(s) 1923
Psp6I CCWGG 3 cut(s) 283, 556, 1031
PspGI CCWGG 3 cut(s) 283, 556, 1031
PspN4I GGNNCC 7 cut(s) 273, 677, 753, 834, 897, 963, 1449
PspPI GGNCC 4 cut(s) 676, 833, 878, 1539
PspXI VCTCGAGB 1 cut(s) 362
PsuI RGATCY 1 cut(s) 1294
PvuII CAGCTG 1 cut(s) 542
RsaI GTAC 6 cut(s) 200, 236, 719, 947, 1535, 1847
RsaNI GTAC 6 cut(s) 199, 235, 718, 946, 1534, 1846
RseI CAYNNNNRTG 1 cut(s) 1179
SacI GAGCTC 2 cut(s) 59, 369
SalI GTCGAC 1 cut(s) 1147
SaqAI TTAA 3 cut(s) 297, 858, 1931
SatI GCNGC 7 cut(s) 152, 181, 410, 540, 633, 647, 1956
Sau3AI GATC 7 cut(s) 11, 496, 582, 1294, 1429, 1510, 1677
Sau96I GGNCC 4 cut(s) 676, 833, 878, 1539
ScaI AGTACT 1 cut(s) 1535
SchI GAGTC 1 cut(s) 522
ScrFI CCNGG 8 cut(s) 285, 342, 504, 528, 558, 966, 1033, 1124
SduI GDGCHC 6 cut(s) 59, 209, 369, 495, 1011, 1903
SfaNI GCATC 4 cut(s) 475, 1060, 1389, 1449
SfcI CTRYAG 2 cut(s) 1142, 2033
Sfr274I CTCGAG 1 cut(s) 362
SfuI TTCGAA 2 cut(s) 28, 403
SinI GGWCC 4 cut(s) 676, 833, 878, 1539
SlaI CTCGAG 1 cut(s) 362
SmiMI CAYNNNNRTG 1 cut(s) 1179
SmlI CTYRAG 2 cut(s) 362, 1874
SmoI CTYRAG 2 cut(s) 362, 1874
SnaBI TACGTA 1 cut(s) 721
SseBI AGGCCT 3 cut(s) 309, 382, 448
SsiI CCGC 3 cut(s) 409, 633, 646
SspMI CTAG 3 cut(s) 1278, 1550, 1866
SstI GAGCTC 2 cut(s) 59, 369
StuI AGGCCT 3 cut(s) 309, 382, 448
StyD4I CCNGG 8 cut(s) 283, 340, 502, 526, 556, 964, 1031, 1122
StyI CCWWGG 1 cut(s) 1277
TaaI ACNGT 2 cut(s) 1146, 1592
TaiI ACGT 4 cut(s) 723, 1014, 1441, 1926
TaqII GACCGA 2 cut(s) 866, 1303
TatI WGTACW 1 cut(s) 1533
TauI GCSGC 3 cut(s) 412, 635, 649
Tru1I TTAA 3 cut(s) 297, 858, 1931
Tru9I TTAA 3 cut(s) 297, 858, 1931
TscAI CASTG 4 cut(s) 214, 436, 1378, 1621
TseFI GTSAC 5 cut(s) 250, 349, 1118, 1157, 1315
TseI GCWGC 4 cut(s) 151, 180, 539, 1955
Tsp45I GTSAC 5 cut(s) 250, 349, 1118, 1157, 1315
TspDTI ATGAA 5 cut(s) 498, 778, 806, 884, 1765
TspGWI ACGGA 1 cut(s) 109
TspRI CASTG 4 cut(s) 214, 436, 1378, 1621
Van91I CCANNNNNTGG 2 cut(s) 1064, 1791
VneI GTGCAC 2 cut(s) 205, 1899
VpaK11BI GGWCC 4 cut(s) 676, 833, 878, 1539
VspI ATTAAT 1 cut(s) 1931
XagI CCTNNNNNAGG 2 cut(s) 816, 1837
XapI RAATTY 5 cut(s) 507, 983, 1191, 1321, 2013
XceI RCATGY 3 cut(s) 205, 1026, 1907
XhoI CTCGAG 1 cut(s) 362
XmaJI CCTAGG 1 cut(s) 1277
XmiI GTMKAC 1 cut(s) 1148
XmnI GAANNNNTTC 1 cut(s) 1941
XspI CTAG 3 cut(s) 1278, 1550, 1866
ZrmI AGTACT 1 cut(s) 1535
Zsp2I ATGCAT 1 cut(s) 627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.