Rh5DG389800

Phospholipid diacylglycerol acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0011775
Physical Location & Seq
Forward (+)
60746430 .. 60750715
4286 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG389800.1

Sequence Viewer

Length: 2067 bp
ATGGCTGCTTCAATTCTTCGGCTTCGCAAGCTGTGCTATGTTCAGCCTGTGAGGTTTTCGGCATTGTGCTTTCAGTCCTTTGAAACAGCCAAGGACGAGGGTGCTGAAACTCCGAAGTTCGAAAATGAAGTGAAAGAGAAACAGAGGATTAAGAACAAGAACAAGAACAAGACGCAACCGAGGGAATGGAGATGCATACATAACTGTTGTTGGATAATTGGGTCCATGTGTACAACTTGGTGGCTTCTCTTGTTTTTGTACAACACCTTGCCAACTGCATTGACTGGTTTTCAGGTGCCAGAATCACCTGGAACAAGGCTTAGGCGCGAAGGTGTAAGCCTCCTTCACCCAGTTGTCATGGTCCCTGGCATTGTAACCGGTGGCCTAGAGCTCTGGGAGGGCAGGCCTTGTGCTGAAGGTCTTTTTAGGAAGCGACTTTGGGGTGGTAGTTTCTCAGAGATCTTCAAAAGGCCTATGTGTTTGTTGGAGCACCTATCACTGGACAATGAAACAGGGCTGGACCCTCCAGGAATTCGAGTCAGAGCAGTTCGGGGACTCGCTGCAGCTGACTATTTTGCTCCTGGCTACTTTGTTTGGGCTGTCCTCATTAAGAATTTGGCAAAAATTGGTTATGAAGGGAAGAACATGCATATGGCTGCCTATGATTGGAGGCTTTCTTTCCAGAACACTGAGATTCGGGACCAAGCTCTTAGTAGGTTGAAGAGTAAAATTGAGCTTATGTATGCAATCAATGGCCATAAGAAAGTGGTCGTGGTGCCTCATTCTATGGGGGCTATTTATTTTCTCCACTTCATGAAATGGGTCGAGTCACCGCCTCCTATGGGAGGTGGTGGTGGTCCAAGTTGGTGTGCCACGCACATCAAAGCAATCATGAATATCGGTCCAGCATTTCTTGGTGTTCCAAGAGCAGTTAGTAATATATTTTCTGCTGAGGGTAAAGATGTTGCTTACATCAGAGCTATGGCTCCTGTTGTTTTAGATTCTGAAATTCTTGGGCTTCAAACCTTTGAACATGTCATGCGGGTATCTCGGAGCTGGGATTCCTTGATTTCCTTGTTGCCAAAAGGTGGAGAAACCATTTGGGGCAACTTGGAGTGGTCTCCTGAAGACGGGCATAGCTGTGACCTGACAAAGAAGAAATATCTGCATTCCACTGCTGGTGATACCAATTACAATGACAGCAATAAGAATATAATTTTCCAAGTAAAAGAACCTATGAAGTATGGAAGAATAATCTCATTTGGCAAGGAATCATCAGAATTACCCTCTTCAGAGCTTCCTGGTCATGATATAAAGGAACTTCTTTACAGGGATTCTCCTACCAATTTCAACTCATCATGTGGAGAGGTGTGGACTGAATATGATGAGGTAAGCAGGGAAAGCATAACAAAAATTGCAGAAAATAAGGCTTACACAGTTCAAACCCTTTTTGATCTGCTACGCTTTGTGGCACCAAAAATGATGCAACGGGCTGAGGCTCACTTCTCTCATGGGATAGCTGATAATCTTGATAATCCTAAATATGACCATTACAAGTACTGGTCCAATCCACTCGAGACCAAGTTACCCATTGCTCCAGATTTGGAAATATACTGTTTATATGGTGTTGGACTCCCAACTGAAAGATCATACATATACAAGACATCTCCTTCTGACAAGTGCAAGAACATCCCATTCCGCATTGATAGCTCAGCAGATGGAGAGACAGGCAGCTGCCTTAAAAATGGAGCACATTTTGTTGATGGTGATGATACCGTGCCTGTATTGAGTGCTGGATACATGTGCACCAAAGGGTGGAGAGGAAGAACCAGGTTCAACCCATCCGGTATTGCTACCTACATAAGGGAGTACCAGCACAAGCGACCTGGTAGTTTTATTGAGGGAAGGGGTACGGAGAGTGGTTCACATGTTGACATCATGGGAAATGTGGCATTAATCGAAGATATCCTATGCATTGCTGCCGGAGCCACTGGTGCACAGATTGGAGGTGATAGGATCTACTCAGATATTATGAGAATGTCTGAGAGAATAAATCTAAAGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 map00561
Pfam Domains
Protein Families
LCAT Lipase_GDSL

Protein Analysis

688

Amino Acids

77.27

Weight (kDa)

8.42

Isoelectric Point (pI)

45.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCAT PF02450 142 - 648 3.1e-65 Lecithin:cholesterol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011775)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G44830
fragaria_vesca FvH4_3g30050
malus_domestica MD11G1154200.v1.1
prunus_persica Prupe.6G116700_v2.0.a1 Prupe.6G116700_v2.0.a1
pyrus_communis pycom11g12590
rosa_chinensis RchiOBHm_Chr5g0055851 RchiOBHm_Chr5g0055861
rosa_laevigata RLG00000035014
rosa_multiflora Rmu_sc0003306.1_g000008
rosa_roxburghii Rroxscaffold_1G00024510
rosa_rugosa Rorug05G0295200 Rorug05G0295200 Rorug05G0295300
rosa_samantha Rh5AG366600 Rh5BG376800 Rh5CG399800 Rh5DG389800
rosa_wichuraiana Rw5G034360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 295, 775, 1471
AccB7I CCANNNNNTGG 2 cut(s) 1088, 1815
AccII CGCG 1 cut(s) 327
AciI CCGC 3 cut(s) 833, 1042, 1699
AclWI GGATC 1 cut(s) 2024
AcoI YGGCCR 1 cut(s) 754
AcsI RAATTY 3 cut(s) 531, 613, 1008
AcuI CTGAAG 3 cut(s) 435, 1146, 1275
AfaI GTAC 5 cut(s) 232, 260, 1559, 1871, 1912
AfiI CCNNNNNNNGG 8 cut(s) 499, 666, 842, 845, 1088, 1130, 1815, 1863
AflIII ACRYGT 3 cut(s) 1033, 1800, 1927
AgeI ACCGGT 1 cut(s) 377
AgsI TTSAA 9 cut(s) 12, 83, 466, 721, 1022, 1031, 1351, 1442, 1837
AjnI CCWGG 7 cut(s) 307, 364, 526, 580, 1300, 1829, 1885
Alw21I GWGCWC 5 cut(s) 393, 492, 1753, 1808, 1999
Alw26I GTCTC 3 cut(s) 1125, 1571, 1718
Alw44I GTGCAC 2 cut(s) 1804, 1995
AlwI GGATC 1 cut(s) 2024
Ama87I CYCGRG 1 cut(s) 1574
AoxI GGCC 4 cut(s) 382, 404, 470, 754
ApaLI GTGCAC 2 cut(s) 1804, 1995
ApeKI GCWGC 7 cut(s) 5, 560, 563, 656, 1731, 1734, 1979
ApoI RAATTY 3 cut(s) 531, 613, 1008
AseI ATTAAT 1 cut(s) 1955
AsiGI ACCGGT 1 cut(s) 377
AspLEI GCGC 1 cut(s) 327
AspS9I GGNCC 7 cut(s) 222, 361, 520, 700, 857, 902, 1563
AsuHPI GGTGA 6 cut(s) 297, 338, 822, 1193, 1778, 2021
AsuII TTCGAA 1 cut(s) 120
AvaI CYCGRG 1 cut(s) 1574
AvaII GGWCC 7 cut(s) 222, 361, 520, 700, 857, 902, 1563
BaeGI GKGCMC 2 cut(s) 1808, 1999
BalI TGGCCA 1 cut(s) 756
BanI GGYRCC 3 cut(s) 295, 775, 1471
BanII GRGCYC 1 cut(s) 393
BarI GAAGNNNNNNTAC 2 cut(s) 327, 359
BbsI GAAGAC 1 cut(s) 1134
Bbv12I GWGCWC 5 cut(s) 393, 492, 1753, 1808, 1999
BbvCI CCTCAGC 2 cut(s) 951, 1494
BbvI GCAGC 6 cut(s) 547, 575, 643, 1721, 1743, 1966
BccI CCATC 3 cut(s) 1712, 1757, 1849
BciT130I CCWGG 7 cut(s) 309, 366, 528, 582, 1302, 1831, 1887
BciVI GTATCC 1 cut(s) 1790
BcoDI GTCTC 3 cut(s) 1125, 1571, 1718
BfaI CTAG 1 cut(s) 386
BfmI CTRYAG 1 cut(s) 561
BfuI GTATCC 1 cut(s) 1790
BglII AGATCT 1 cut(s) 459
BisI GCNGC 7 cut(s) 6, 561, 564, 657, 1732, 1735, 1980
BlpI GCTNAGC 1 cut(s) 1711
BlsI GCNGC 7 cut(s) 7, 562, 565, 658, 1733, 1736, 1981
BmcAI AGTACT 1 cut(s) 1559
Bme1390I CCNGG 7 cut(s) 309, 366, 528, 582, 1302, 1831, 1887
Bme18I GGWCC 7 cut(s) 222, 361, 520, 700, 857, 902, 1563
BmeT110I CYCGRG 1 cut(s) 1574
BmgT120I GGNCC 7 cut(s) 222, 361, 520, 700, 857, 902, 1563
BmiI GGNNCC 9 cut(s) 223, 297, 363, 522, 701, 777, 987, 1473, 1987
BmrFI CCNGG 7 cut(s) 309, 366, 528, 582, 1302, 1831, 1887
BmrI ACTGGG 1 cut(s) 344
BmsI GCATC 2 cut(s) 182, 1473
BmuI ACTGGG 1 cut(s) 344
BpiI GAAGAC 1 cut(s) 1134
BpmI CTGGAG 2 cut(s) 510, 1581
Bpu10I CCTNAGC 3 cut(s) 320, 951, 1494
Bpu1102I GCTNAGC 1 cut(s) 1711
Bpu14I TTCGAA 1 cut(s) 120
BsaI GGTCTC 2 cut(s) 1125, 1571
BsaJI CCNNGG 3 cut(s) 90, 179, 364
BsaWI WCCGGW 2 cut(s) 377, 1844
Bsc4I CCNNNNNNNGG 8 cut(s) 499, 666, 842, 845, 1088, 1130, 1815, 1863
Bse118I RCCGGY 1 cut(s) 377
Bse1I ACTGG 5 cut(s) 289, 350, 504, 1565, 1996
Bse3DI GCAATG 2 cut(s) 1590, 1974
BseBI CCWGG 7 cut(s) 309, 366, 528, 582, 1302, 1831, 1887
BseDI CCNNGG 3 cut(s) 90, 179, 364
BseGI GGATG 2 cut(s) 1689, 1841
BseLI CCNNNNNNNGG 8 cut(s) 499, 666, 842, 845, 1088, 1130, 1815, 1863
BseMI GCAATG 2 cut(s) 1590, 1974
BseMII CTCAG 7 cut(s) 468, 681, 942, 1485, 1725, 2034, 2037
BseNI ACTGG 5 cut(s) 289, 350, 504, 1565, 1996
BseSI GKGCMC 2 cut(s) 1808, 1999
BseXI GCAGC 6 cut(s) 547, 575, 643, 1721, 1743, 1966
BseYI CCCAGC 1 cut(s) 1056
Bsh1236I CGCG 1 cut(s) 327
BshFI GGCC 4 cut(s) 384, 406, 472, 756
BshNI GGYRCC 3 cut(s) 295, 775, 1471
BshTI ACCGGT 1 cut(s) 377
BsiHKAI GWGCWC 5 cut(s) 393, 492, 1753, 1808, 1999
BsiHKCI CYCGRG 1 cut(s) 1574
BsiSI CCGG 3 cut(s) 378, 1845, 1983
BslFI GGGAC 3 cut(s) 347, 567, 713
BslI CCNNNNNNNGG 8 cut(s) 499, 666, 842, 845, 1088, 1130, 1815, 1863
BsmAI GTCTC 3 cut(s) 1125, 1571, 1718
BsmFI GGGAC 3 cut(s) 347, 567, 713
BsmI GAATGC 1 cut(s) 1168
BsnI GGCC 4 cut(s) 384, 406, 472, 756
Bso31I GGTCTC 2 cut(s) 1125, 1571
BsoBI CYCGRG 1 cut(s) 1574
Bsp119I TTCGAA 1 cut(s) 120
Bsp1286I GDGCHC 5 cut(s) 393, 492, 1753, 1808, 1999
Bsp1407I TGTACA 2 cut(s) 230, 258
Bsp143I GATC 4 cut(s) 459, 1453, 1646, 2016
Bsp1720I GCTNAGC 1 cut(s) 1711
BspACI CCGC 3 cut(s) 833, 1042, 1699
BspANI GGCC 4 cut(s) 384, 406, 472, 756
BspCNI CTCAG 7 cut(s) 467, 682, 943, 1486, 1724, 2035, 2036
BspFNI CGCG 1 cut(s) 327
BspHI TCATGA 3 cut(s) 813, 891, 1306
BspLI GGNNCC 9 cut(s) 223, 297, 363, 522, 701, 777, 987, 1473, 1987
BspMAI CTGCAG 1 cut(s) 565
BspPI GGATC 1 cut(s) 2024
BspT104I TTCGAA 1 cut(s) 120
BspT107I GGYRCC 3 cut(s) 295, 775, 1471
BspTNI GGTCTC 2 cut(s) 1125, 1571
BsrDI GCAATG 2 cut(s) 1590, 1974
BsrFI RCCGGY 1 cut(s) 377
BsrGI TGTACA 2 cut(s) 230, 258
BsrI ACTGG 5 cut(s) 289, 350, 504, 1565, 1996
BssAI RCCGGY 1 cut(s) 377
BssECI CCNNGG 3 cut(s) 90, 179, 364
BssMI GATC 4 cut(s) 459, 1453, 1646, 2016
BssT1I CCWWGG 1 cut(s) 90
Bst2UI CCWGG 7 cut(s) 309, 366, 528, 582, 1302, 1831, 1887
Bst4CI ACNGT 4 cut(s) 206, 1438, 1616, 1777
Bst6I CTCTTC 2 cut(s) 716, 1294
BstAPI GCANNNNNTGC 1 cut(s) 33
BstAUI TGTACA 2 cut(s) 230, 258
BstBI TTCGAA 1 cut(s) 120
BstC8I GCNNGC 2 cut(s) 29, 404
BstDEI CTNAG 9 cut(s) 320, 454, 690, 710, 951, 1494, 1711, 2023, 2043
BstENI CCTNNNNNAGG 2 cut(s) 843, 1861
BstF5I GGATG 2 cut(s) 1689, 1841
BstFNI CGCG 1 cut(s) 327
BstHHI GCGC 1 cut(s) 327
BstKTI GATC 4 cut(s) 462, 1456, 1649, 2019
BstMAI GTCTC 3 cut(s) 1125, 1571, 1718
BstMBI GATC 4 cut(s) 459, 1453, 1646, 2016
BstMWI GCNNNNNNNGC 6 cut(s) 28, 33, 1401, 1707, 1985, 1994
BstNI CCWGG 7 cut(s) 309, 366, 528, 582, 1302, 1831, 1887
BstNSI RCATGY 4 cut(s) 649, 1037, 1804, 1931
BstSCI CCNGG 7 cut(s) 307, 364, 526, 580, 1300, 1829, 1885
BstSFI CTRYAG 1 cut(s) 561
BstSLI GKGCMC 2 cut(s) 1808, 1999
BstUI CGCG 1 cut(s) 327
BstV1I GCAGC 6 cut(s) 547, 575, 643, 1721, 1743, 1966
BstV2I GAAGAC 1 cut(s) 1134
BstX2I RGATCY 2 cut(s) 459, 2016
BstYI RGATCY 2 cut(s) 459, 2016
BsuI GTATCC 1 cut(s) 1790
BsuRI GGCC 4 cut(s) 384, 406, 472, 756
BtsCI GGATG 2 cut(s) 1689, 1841
BtsI GCAGTG 1 cut(s) 1173
BtsIMutI CAGTG 4 cut(s) 497, 687, 1173, 1989
Cac8I GCNNGC 2 cut(s) 29, 404
CciI TCATGA 3 cut(s) 813, 891, 1306
CfoI GCGC 1 cut(s) 327
Cfr10I RCCGGY 1 cut(s) 377
Cfr13I GGNCC 7 cut(s) 222, 361, 520, 700, 857, 902, 1563
CseI GACGC 1 cut(s) 181
CsiI ACCWGGT 2 cut(s) 1829, 1885
Csp6I GTAC 5 cut(s) 231, 259, 1558, 1870, 1911
CspAI ACCGGT 1 cut(s) 377
CviQI GTAC 5 cut(s) 231, 259, 1558, 1870, 1911
DdeI CTNAG 9 cut(s) 320, 454, 690, 710, 951, 1494, 1711, 2023, 2043
DpnI GATC 4 cut(s) 461, 1455, 1648, 2018
DpnII GATC 4 cut(s) 459, 1453, 1646, 2016
EaeI YGGCCR 1 cut(s) 754
Eam1104I CTCTTC 2 cut(s) 716, 1294
EarI CTCTTC 2 cut(s) 716, 1294
Ecl136II GAGCTC 1 cut(s) 391
Eco130I CCWWGG 1 cut(s) 90
Eco147I AGGCCT 2 cut(s) 406, 472
Eco24I GRGCYC 1 cut(s) 393
Eco31I GGTCTC 2 cut(s) 1125, 1571
Eco32I GATATC 1 cut(s) 1966
Eco47I GGWCC 7 cut(s) 222, 361, 520, 700, 857, 902, 1563
Eco53kI GAGCTC 1 cut(s) 391
Eco57I CTGAAG 3 cut(s) 435, 1146, 1275
Eco88I CYCGRG 1 cut(s) 1574
EcoICRI GAGCTC 1 cut(s) 391
EcoNI CCTNNNNNAGG 2 cut(s) 843, 1861
EcoRI GAATTC 1 cut(s) 531
EcoRII CCWGG 7 cut(s) 307, 364, 526, 580, 1300, 1829, 1885
EcoRV GATATC 1 cut(s) 1966
EcoT14I CCWWGG 1 cut(s) 90
EcoT22I ATGCAT 3 cut(s) 197, 651, 1976
EcoT38I GRGCYC 1 cut(s) 393
ErhI CCWWGG 1 cut(s) 90
FaqI GGGAC 3 cut(s) 347, 567, 713
FauI CCCGC 1 cut(s) 1035
FauNDI CATATG 1 cut(s) 651
Fnu4HI GCNGC 7 cut(s) 6, 561, 564, 657, 1732, 1735, 1980
FokI GGATG 2 cut(s) 1676, 1828
FriOI GRGCYC 1 cut(s) 393
Fsp4HI GCNGC 7 cut(s) 6, 561, 564, 657, 1732, 1735, 1980
FspBI CTAG 1 cut(s) 386
GlaI GCGC 1 cut(s) 326
GluI GCNGC 7 cut(s) 6, 561, 564, 657, 1732, 1735, 1980
GsaI CCCAGC 1 cut(s) 1060
GsuI CTGGAG 2 cut(s) 510, 1581
HaeIII GGCC 4 cut(s) 384, 406, 472, 756
HapII CCGG 3 cut(s) 378, 1845, 1983
HgaI GACGC 1 cut(s) 181
HhaI GCGC 1 cut(s) 327
Hin6I GCGC 1 cut(s) 325
HinP1I GCGC 1 cut(s) 325
HincII GTYRAC 1 cut(s) 1933
HindII GTYRAC 1 cut(s) 1933
HpaII CCGG 3 cut(s) 378, 1845, 1983
HphI GGTGA 6 cut(s) 297, 338, 822, 1193, 1778, 2021
Hpy166II GTNNAC 6 cut(s) 231, 1374, 1806, 1925, 1933, 1997
Hpy188III TCNNGA 9 cut(s) 682, 698, 814, 892, 1124, 1307, 1529, 1576, 1598
Hpy8I GTNNAC 6 cut(s) 231, 1374, 1806, 1925, 1933, 1997
HpyAV CCTTC 6 cut(s) 323, 353, 410, 629, 1680, 1899
HpyCH4III ACNGT 4 cut(s) 206, 1438, 1616, 1777
HpyF10VI GCNNNNNNNGC 6 cut(s) 28, 33, 1401, 1707, 1985, 1994
HpyF3I CTNAG 9 cut(s) 320, 454, 690, 710, 951, 1494, 1711, 2023, 2043
HspAI GCGC 1 cut(s) 325
Kzo9I GATC 4 cut(s) 459, 1453, 1646, 2016
LmnI GCTCC 7 cut(s) 487, 583, 991, 1053, 1600, 1748, 1985
Lsp1109I GCAGC 6 cut(s) 547, 575, 643, 1721, 1743, 1966
LweI GCATC 2 cut(s) 182, 1473
MabI ACCWGGT 2 cut(s) 1829, 1885
MaeI CTAG 1 cut(s) 386
MaeIII GTNAC 4 cut(s) 373, 828, 1142, 1584
MalI GATC 4 cut(s) 461, 1455, 1648, 2018
MboI GATC 4 cut(s) 459, 1453, 1646, 2016
MflI RGATCY 2 cut(s) 459, 2016
MhlI GDGCHC 5 cut(s) 393, 492, 1753, 1808, 1999
MlsI TGGCCA 1 cut(s) 756
MluNI TGGCCA 1 cut(s) 756
MlyI GAGTC 4 cut(s) 546, 549, 836, 1626
MmeI TCCRAC 3 cut(s) 191, 465, 1609
Mox20I TGGCCA 1 cut(s) 756
Mph1103I ATGCAT 3 cut(s) 197, 651, 1976
MscI TGGCCA 1 cut(s) 756
MseI TTAA 4 cut(s) 150, 609, 1740, 1955
MslI CAYNNNNRTG 2 cut(s) 650, 1140
Msp20I TGGCCA 1 cut(s) 756
MspA1I CMGCKG 2 cut(s) 566, 1734
MspI CCGG 3 cut(s) 378, 1845, 1983
MspR9I CCNGG 7 cut(s) 309, 366, 528, 582, 1302, 1831, 1887
Mva1269I GAATGC 1 cut(s) 1168
MvaI CCWGG 7 cut(s) 309, 366, 528, 582, 1302, 1831, 1887
MvnI CGCG 1 cut(s) 327
MwoI GCNNNNNNNGC 6 cut(s) 28, 33, 1401, 1707, 1985, 1994
NdeI CATATG 1 cut(s) 651
NdeII GATC 4 cut(s) 459, 1453, 1646, 2016
NlaIV GGNNCC 9 cut(s) 223, 297, 363, 522, 701, 777, 987, 1473, 1987
NmuCI GTSAC 2 cut(s) 828, 1142
NsiI ATGCAT 3 cut(s) 197, 651, 1976
NspI RCATGY 4 cut(s) 649, 1037, 1804, 1931
NspV TTCGAA 1 cut(s) 120
PaeR7I CTCGAG 1 cut(s) 1574
PagI TCATGA 3 cut(s) 813, 891, 1306
PceI AGGCCT 2 cut(s) 406, 472
PciI ACATGT 3 cut(s) 1033, 1800, 1927
PctI GAATGC 1 cut(s) 1168
PfeI GAWTC 6 cut(s) 302, 694, 1001, 1061, 1271, 1334
PflMI CCANNNNNTGG 2 cut(s) 1088, 1815
PfoI TCCNGGA 1 cut(s) 526
PinAI ACCGGT 1 cut(s) 377
PkrI GCNGC 7 cut(s) 7, 562, 565, 658, 1733, 1736, 1981
PleI GAGTC 4 cut(s) 545, 549, 835, 1626
PpsI GAGTC 4 cut(s) 545, 549, 835, 1626
PscI ACATGT 3 cut(s) 1033, 1800, 1927
PshBI ATTAAT 1 cut(s) 1955
Psp124BI GAGCTC 1 cut(s) 393
Psp6I CCWGG 7 cut(s) 307, 364, 526, 580, 1300, 1829, 1885
PspFI CCCAGC 1 cut(s) 1056
PspGI CCWGG 7 cut(s) 307, 364, 526, 580, 1300, 1829, 1885
PspN4I GGNNCC 9 cut(s) 223, 297, 363, 522, 701, 777, 987, 1473, 1987
PspPI GGNCC 7 cut(s) 222, 361, 520, 700, 857, 902, 1563
PstI CTGCAG 1 cut(s) 565
PsuI RGATCY 2 cut(s) 459, 2016
PvuII CAGCTG 2 cut(s) 566, 1734
RsaI GTAC 5 cut(s) 232, 260, 1559, 1871, 1912
RsaNI GTAC 5 cut(s) 231, 259, 1558, 1870, 1911
RseI CAYNNNNRTG 2 cut(s) 650, 1140
SacI GAGCTC 1 cut(s) 393
SaqAI TTAA 4 cut(s) 150, 609, 1740, 1955
SatI GCNGC 7 cut(s) 6, 561, 564, 657, 1732, 1735, 1980
Sau3AI GATC 4 cut(s) 459, 1453, 1646, 2016
Sau96I GGNCC 7 cut(s) 222, 361, 520, 700, 857, 902, 1563
ScaI AGTACT 1 cut(s) 1559
SchI GAGTC 4 cut(s) 546, 549, 836, 1626
ScrFI CCNGG 7 cut(s) 309, 366, 528, 582, 1302, 1831, 1887
SduI GDGCHC 5 cut(s) 393, 492, 1753, 1808, 1999
SexAI ACCWGGT 2 cut(s) 1829, 1885
SfaNI GCATC 2 cut(s) 182, 1473
SfcI CTRYAG 1 cut(s) 561
Sfr274I CTCGAG 1 cut(s) 1574
SfuI TTCGAA 1 cut(s) 120
SinI GGWCC 7 cut(s) 222, 361, 520, 700, 857, 902, 1563
SlaI CTCGAG 1 cut(s) 1574
SmiMI CAYNNNNRTG 2 cut(s) 650, 1140
SmlI CTYRAG 1 cut(s) 1574
SmoI CTYRAG 1 cut(s) 1574
SseBI AGGCCT 2 cut(s) 406, 472
SsiI CCGC 3 cut(s) 833, 1042, 1699
SspMI CTAG 1 cut(s) 386
SstI GAGCTC 1 cut(s) 393
StuI AGGCCT 2 cut(s) 406, 472
StyD4I CCNGG 7 cut(s) 307, 364, 526, 580, 1300, 1829, 1885
StyI CCWWGG 1 cut(s) 90
TaaI ACNGT 4 cut(s) 206, 1438, 1616, 1777
TaqI TCGA 5 cut(s) 120, 535, 825, 1575, 1959
TaqII GACCGA 1 cut(s) 890
TatI WGTACW 3 cut(s) 230, 258, 1557
TfiI GAWTC 6 cut(s) 302, 694, 1001, 1061, 1271, 1334
Tru1I TTAA 4 cut(s) 150, 609, 1740, 1955
Tru9I TTAA 4 cut(s) 150, 609, 1740, 1955
TscAI CASTG 4 cut(s) 504, 694, 1180, 1996
TseFI GTSAC 2 cut(s) 828, 1142
TseI GCWGC 7 cut(s) 5, 560, 563, 656, 1731, 1734, 1979
Tsp45I GTSAC 2 cut(s) 828, 1142
TspDTI ATGAA 7 cut(s) 141, 522, 648, 802, 830, 908, 1253
TspGWI ACGGA 1 cut(s) 1928
TspRI CASTG 4 cut(s) 504, 694, 1180, 1996
Van91I CCANNNNNTGG 2 cut(s) 1088, 1815
VneI GTGCAC 2 cut(s) 1804, 1995
VpaK11BI GGWCC 7 cut(s) 222, 361, 520, 700, 857, 902, 1563
VspI ATTAAT 1 cut(s) 1955
XagI CCTNNNNNAGG 2 cut(s) 843, 1861
XapI RAATTY 3 cut(s) 531, 613, 1008
XceI RCATGY 4 cut(s) 649, 1037, 1804, 1931
XhoI CTCGAG 1 cut(s) 1574
XspI CTAG 1 cut(s) 386
ZrmI AGTACT 1 cut(s) 1559
Zsp2I ATGCAT 3 cut(s) 197, 651, 1976
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.