Rh5BG376800

Phospholipid diacylglycerol acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0011775
Physical Location & Seq
Forward (+)
59613754 .. 59618162
4409 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG376800.1

Sequence Viewer

Length: 1581 bp
ATGGCTGCTTCAATTCTTCGGCTTCGCAAGCCGTGCTATGTTCAGCCTGTGGGGTTTTCGGCATTGTGCTTTCAGTCCTTTGAAACAGCCAAGGACGAGGGTGCTGAAACCCCGAAGTTCGAAAATGAAGTGAAAGAGAGACAGAGGATTAAGAACAAGAACATGAAGCAACCGAGGGAATGGAGATGCATACATAACTGTTGTTGGATAATTGGGTCCATGTGTACAACTTGGTGGCTTCTCTTGTTTTTGTATAACACCTTGCCAACTGCATTGACTGGTTTTCAGGTGCCAGAATCACCTGGAACAAGGCTTAGGCGCGAAGGTGTAAGCCCGCTTCACCCAGTTGTCATGGTCCCTGGCATTGTAACCGGTGGCCTAGAGCTCTGGGAGGGCAGGCCTTGTGCTGAAGGTCTTTTTAGGAAGCGACTTTGGGGTGGTAGTTTCTCAGAGATCTTCAAAAGGCCTATGTGTTTGTTGGAGCACCTATCACTGGACAATGAAACAGGGCTGGACCCTCCAGGAATTCGAGTCAGAGCAGTTCGGGGACTCGCTGCAGCTGACTATTTTGCTCCTGGCTACTTTGTTTGGGCTGTCCTCATTAAGAATTTGGCAAAAATTGGTTATGAAGGGAAGAACATGCATATGGCTGCCTATGATTGGAGGCTTTCTTTCCAGAACACTGAGATTCGGGACCAAGCTCTTAGTAGGTTGAAGAGTAAAATTGAGCTTATGTATGCAATCAATGGCCATAAGAAAGTGGTCGTGGTGCCTCATTCTATGGGGGCTATTTATTTTCTCCACTTCATGAAATGGGTTGAGTCACCTCCTCCTATGGGAGGTGGTGGTGGTCCAAGTTGGTGTGCCACGCACATCAAAGCAATCATGAATATCGGTCCAGCATTTCTTGGTGTTCCAAGAGCAGTTAGTAATATATTTTCTGCTGAGGGTAAAGATGTTGCTTACATCAGAGCTATGGCTCCTGTTGTTTTAGATTCTGAAATTCTTGGGCTTCAAACCTTTGAACGTGTCATGCGGGTATCTCGGAGCTGGGATTCCTTGATTTCCTTGTTGCCAAAAGGTGGAGAAACCATTTGGGGCAACTTGGAGTGGTCTCCTGAAGACGGGCATAGCTGTGACCTGACAAAGAAGAAATATCTGCATTCCACTGCTGGTGATACCAATTACAACAACAGCAATAAGAATATAATTTTCCAAATAAAAGAACCTATGAAGTATGGAAGAATAATCTCATTTGGCAAGGAATCATCAGAATTACCCTCTTCAGAGCTTCCTGGTCATGATATAAAGGAACTTCTTTACAGGGATTCTCCTACCAATTTCAACTCATCATGTGGAGAGGTGTGGACTGAATATGATGAGGTAAGCAGGGAAAGCATAAGAAAAATTGCAGAAAATAAGGCTTACACAGTTCAAACCCTTTTTGATCTGCTACGCTTTGTGGCACCAAAAATGATGCAACGGGCTGAGGCTCACTTCTCTCATGGGATAGCTGATAATCTTGATAATCCTAAATATGACCATTACAAGTACTGGTCCAATCCACTCGAGACCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 map00561
Pfam Domains
Protein Families
LCAT Lipase_GDSL

Protein Analysis

526

Amino Acids

59.61

Weight (kDa)

8.77

Isoelectric Point (pI)

45.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCAT PF02450 140 - 376 8.8e-45 Lecithin:cholesterol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011775)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G44830
fragaria_vesca FvH4_3g30050
malus_domestica MD11G1154200.v1.1
prunus_persica Prupe.6G116700_v2.0.a1 Prupe.6G116700_v2.0.a1
pyrus_communis pycom11g12590
rosa_chinensis RchiOBHm_Chr5g0055851 RchiOBHm_Chr5g0055861
rosa_laevigata RLG00000035014
rosa_multiflora Rmu_sc0003306.1_g000008
rosa_roxburghii Rroxscaffold_1G00024510
rosa_rugosa Rorug05G0295200 Rorug05G0295200 Rorug05G0295300
rosa_samantha Rh5AG366600 Rh5BG376800 Rh5CG399800 Rh5DG389800
rosa_wichuraiana Rw5G034360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 289, 769, 1465
AccB7I CCANNNNNTGG 1 cut(s) 1082
AccII CGCG 1 cut(s) 321
AciI CCGC 2 cut(s) 335, 1036
AcoI YGGCCR 1 cut(s) 748
AcsI RAATTY 3 cut(s) 525, 607, 1002
AcuI CTGAAG 3 cut(s) 429, 1140, 1269
AfaI GTAC 2 cut(s) 226, 1553
AfiI CCNNNNNNNGG 6 cut(s) 493, 660, 836, 839, 1082, 1124
AflIII ACRYGT 1 cut(s) 1027
AgeI ACCGGT 1 cut(s) 371
AgsI TTSAA 8 cut(s) 12, 83, 460, 715, 1016, 1025, 1345, 1436
AjnI CCWGG 5 cut(s) 301, 358, 520, 574, 1294
AluBI AGCT 9 cut(s) 385, 560, 701, 730, 974, 1050, 1134, 1291, 1514
AluI AGCT 9 cut(s) 385, 560, 701, 730, 974, 1050, 1134, 1291, 1514
Alw21I GWGCWC 2 cut(s) 387, 486
Alw26I GTCTC 3 cut(s) 133, 1119, 1565
Ama87I CYCGRG 1 cut(s) 1568
AoxI GGCC 4 cut(s) 376, 398, 464, 748
ApeKI GCWGC 4 cut(s) 5, 554, 557, 650
ApoI RAATTY 3 cut(s) 525, 607, 1002
AsiGI ACCGGT 1 cut(s) 371
AspLEI GCGC 1 cut(s) 321
AspS9I GGNCC 7 cut(s) 216, 355, 514, 694, 851, 896, 1557
AsuHPI GGTGA 4 cut(s) 291, 332, 816, 1187
AsuII TTCGAA 1 cut(s) 120
AvaI CYCGRG 1 cut(s) 1568
AvaII GGWCC 7 cut(s) 216, 355, 514, 694, 851, 896, 1557
BalI TGGCCA 1 cut(s) 750
BanI GGYRCC 3 cut(s) 289, 769, 1465
BanII GRGCYC 1 cut(s) 387
BarI GAAGNNNNNNTAC 2 cut(s) 321, 353
BbsI GAAGAC 1 cut(s) 1128
Bbv12I GWGCWC 2 cut(s) 387, 486
BbvCI CCTCAGC 2 cut(s) 945, 1488
BbvI GCAGC 3 cut(s) 541, 569, 637
BceAI ACGGC 1 cut(s) 16
BciT130I CCWGG 5 cut(s) 303, 360, 522, 576, 1296
BcoDI GTCTC 3 cut(s) 133, 1119, 1565
BfaI CTAG 1 cut(s) 380
BfmI CTRYAG 1 cut(s) 555
BglII AGATCT 1 cut(s) 453
BisI GCNGC 4 cut(s) 6, 555, 558, 651
BlsI GCNGC 4 cut(s) 7, 556, 559, 652
BmcAI AGTACT 1 cut(s) 1553
Bme1390I CCNGG 5 cut(s) 303, 360, 522, 576, 1296
Bme18I GGWCC 7 cut(s) 216, 355, 514, 694, 851, 896, 1557
BmeT110I CYCGRG 1 cut(s) 1568
BmgT120I GGNCC 7 cut(s) 216, 355, 514, 694, 851, 896, 1557
BmiI GGNNCC 8 cut(s) 217, 291, 357, 516, 695, 771, 981, 1467
BmrFI CCNGG 5 cut(s) 303, 360, 522, 576, 1296
BmrI ACTGGG 1 cut(s) 338
BmsI GCATC 2 cut(s) 176, 1467
BmuI ACTGGG 1 cut(s) 338
BpiI GAAGAC 1 cut(s) 1128
BpmI CTGGAG 1 cut(s) 504
Bpu10I CCTNAGC 3 cut(s) 314, 945, 1488
Bpu14I TTCGAA 1 cut(s) 120
BsaI GGTCTC 2 cut(s) 1119, 1565
BsaJI CCNNGG 3 cut(s) 90, 173, 358
BsaWI WCCGGW 1 cut(s) 371
Bsc4I CCNNNNNNNGG 6 cut(s) 493, 660, 836, 839, 1082, 1124
Bse118I RCCGGY 1 cut(s) 371
Bse1I ACTGG 4 cut(s) 283, 344, 498, 1559
BseBI CCWGG 5 cut(s) 303, 360, 522, 576, 1296
BseDI CCNNGG 3 cut(s) 90, 173, 358
BseLI CCNNNNNNNGG 6 cut(s) 493, 660, 836, 839, 1082, 1124
BseMII CTCAG 4 cut(s) 462, 675, 936, 1479
BseNI ACTGG 4 cut(s) 283, 344, 498, 1559
BseRI GAGGAG 1 cut(s) 819
BseXI GCAGC 3 cut(s) 541, 569, 637
BseYI CCCAGC 1 cut(s) 1050
Bsh1236I CGCG 1 cut(s) 321
BshFI GGCC 4 cut(s) 378, 400, 466, 750
BshNI GGYRCC 3 cut(s) 289, 769, 1465
BshTI ACCGGT 1 cut(s) 371
BsiHKAI GWGCWC 2 cut(s) 387, 486
BsiHKCI CYCGRG 1 cut(s) 1568
BsiSI CCGG 1 cut(s) 372
BslFI GGGAC 3 cut(s) 341, 561, 707
BslI CCNNNNNNNGG 6 cut(s) 493, 660, 836, 839, 1082, 1124
BsmAI GTCTC 3 cut(s) 133, 1119, 1565
BsmFI GGGAC 3 cut(s) 341, 561, 707
BsmI GAATGC 1 cut(s) 1162
BsnI GGCC 4 cut(s) 378, 400, 466, 750
Bso31I GGTCTC 2 cut(s) 1119, 1565
BsoBI CYCGRG 1 cut(s) 1568
Bsp119I TTCGAA 1 cut(s) 120
Bsp1286I GDGCHC 2 cut(s) 387, 486
Bsp1407I TGTACA 1 cut(s) 224
Bsp143I GATC 2 cut(s) 453, 1447
BspACI CCGC 2 cut(s) 335, 1036
BspANI GGCC 4 cut(s) 378, 400, 466, 750
BspCNI CTCAG 4 cut(s) 461, 676, 937, 1480
BspFNI CGCG 1 cut(s) 321
BspHI TCATGA 3 cut(s) 807, 885, 1300
BspLI GGNNCC 8 cut(s) 217, 291, 357, 516, 695, 771, 981, 1467
BspMAI CTGCAG 1 cut(s) 559
BspT104I TTCGAA 1 cut(s) 120
BspT107I GGYRCC 3 cut(s) 289, 769, 1465
BspTNI GGTCTC 2 cut(s) 1119, 1565
BsrFI RCCGGY 1 cut(s) 371
BsrGI TGTACA 1 cut(s) 224
BsrI ACTGG 4 cut(s) 283, 344, 498, 1559
BssAI RCCGGY 1 cut(s) 371
BssECI CCNNGG 3 cut(s) 90, 173, 358
BssMI GATC 2 cut(s) 453, 1447
BssT1I CCWWGG 1 cut(s) 90
Bst2UI CCWGG 5 cut(s) 303, 360, 522, 576, 1296
Bst4CI ACNGT 2 cut(s) 200, 1432
Bst6I CTCTTC 2 cut(s) 710, 1288
BstAPI GCANNNNNTGC 1 cut(s) 33
BstAUI TGTACA 1 cut(s) 224
BstBI TTCGAA 1 cut(s) 120
BstC8I GCNNGC 3 cut(s) 29, 335, 398
BstDEI CTNAG 6 cut(s) 314, 448, 684, 704, 945, 1488
BstENI CCTNNNNNAGG 1 cut(s) 837
BstFNI CGCG 1 cut(s) 321
BstHHI GCGC 1 cut(s) 321
BstKTI GATC 2 cut(s) 456, 1450
BstMAI GTCTC 3 cut(s) 133, 1119, 1565
BstMBI GATC 2 cut(s) 453, 1447
BstMWI GCNNNNNNNGC 3 cut(s) 28, 33, 1395
BstNI CCWGG 5 cut(s) 303, 360, 522, 576, 1296
BstNSI RCATGY 1 cut(s) 643
BstSCI CCNGG 5 cut(s) 301, 358, 520, 574, 1294
BstSFI CTRYAG 1 cut(s) 555
BstUI CGCG 1 cut(s) 321
BstV1I GCAGC 3 cut(s) 541, 569, 637
BstV2I GAAGAC 1 cut(s) 1128
BstX2I RGATCY 1 cut(s) 453
BstYI RGATCY 1 cut(s) 453
BsuRI GGCC 4 cut(s) 378, 400, 466, 750
BtsI GCAGTG 1 cut(s) 1167
BtsIMutI CAGTG 3 cut(s) 491, 681, 1167
Cac8I GCNNGC 3 cut(s) 29, 335, 398
CciI TCATGA 3 cut(s) 807, 885, 1300
CfoI GCGC 1 cut(s) 321
Cfr10I RCCGGY 1 cut(s) 371
Cfr13I GGNCC 7 cut(s) 216, 355, 514, 694, 851, 896, 1557
Csp6I GTAC 2 cut(s) 225, 1552
CspAI ACCGGT 1 cut(s) 371
CviQI GTAC 2 cut(s) 225, 1552
DdeI CTNAG 6 cut(s) 314, 448, 684, 704, 945, 1488
DpnI GATC 2 cut(s) 455, 1449
DpnII GATC 2 cut(s) 453, 1447
EaeI YGGCCR 1 cut(s) 748
Eam1104I CTCTTC 2 cut(s) 710, 1288
EarI CTCTTC 2 cut(s) 710, 1288
Ecl136II GAGCTC 1 cut(s) 385
Eco130I CCWWGG 1 cut(s) 90
Eco147I AGGCCT 2 cut(s) 400, 466
Eco24I GRGCYC 1 cut(s) 387
Eco31I GGTCTC 2 cut(s) 1119, 1565
Eco47I GGWCC 7 cut(s) 216, 355, 514, 694, 851, 896, 1557
Eco53kI GAGCTC 1 cut(s) 385
Eco57I CTGAAG 3 cut(s) 429, 1140, 1269
Eco88I CYCGRG 1 cut(s) 1568
EcoICRI GAGCTC 1 cut(s) 385
EcoNI CCTNNNNNAGG 1 cut(s) 837
EcoRI GAATTC 1 cut(s) 525
EcoRII CCWGG 5 cut(s) 301, 358, 520, 574, 1294
EcoT14I CCWWGG 1 cut(s) 90
EcoT22I ATGCAT 2 cut(s) 191, 645
EcoT38I GRGCYC 1 cut(s) 387
ErhI CCWWGG 1 cut(s) 90
FaqI GGGAC 3 cut(s) 341, 561, 707
FauI CCCGC 2 cut(s) 342, 1029
FauNDI CATATG 1 cut(s) 645
Fnu4HI GCNGC 4 cut(s) 6, 555, 558, 651
FriOI GRGCYC 1 cut(s) 387
Fsp4HI GCNGC 4 cut(s) 6, 555, 558, 651
FspBI CTAG 1 cut(s) 380
GlaI GCGC 1 cut(s) 320
GluI GCNGC 4 cut(s) 6, 555, 558, 651
GsaI CCCAGC 1 cut(s) 1054
GsuI CTGGAG 1 cut(s) 504
HaeIII GGCC 4 cut(s) 378, 400, 466, 750
HapII CCGG 1 cut(s) 372
HhaI GCGC 1 cut(s) 321
Hin6I GCGC 1 cut(s) 319
HinP1I GCGC 1 cut(s) 319
HinfI GANTC 9 cut(s) 296, 531, 549, 688, 821, 995, 1055, 1265, 1328
HpaII CCGG 1 cut(s) 372
HphI GGTGA 4 cut(s) 291, 332, 816, 1187
Hpy166II GTNNAC 2 cut(s) 225, 1368
Hpy188I TCNGA 7 cut(s) 451, 536, 971, 1000, 1047, 1273, 1288
Hpy188III TCNNGA 8 cut(s) 676, 692, 808, 886, 1118, 1301, 1523, 1570
Hpy8I GTNNAC 2 cut(s) 225, 1368
HpyAV CCTTC 3 cut(s) 317, 404, 623
HpyCH4III ACNGT 2 cut(s) 200, 1432
HpyCH4IV ACGT 1 cut(s) 1027
HpyCH4V TGCA 8 cut(s) 189, 272, 557, 643, 740, 1162, 1412, 1480
HpyF10VI GCNNNNNNNGC 3 cut(s) 28, 33, 1395
HpyF3I CTNAG 6 cut(s) 314, 448, 684, 704, 945, 1488
HpySE526I ACGT 1 cut(s) 1027
HspAI GCGC 1 cut(s) 319
Kzo9I GATC 2 cut(s) 453, 1447
LmnI GCTCC 4 cut(s) 481, 577, 985, 1047
Lsp1109I GCAGC 3 cut(s) 541, 569, 637
LweI GCATC 2 cut(s) 176, 1467
MaeI CTAG 1 cut(s) 380
MaeII ACGT 1 cut(s) 1027
MaeIII GTNAC 3 cut(s) 367, 822, 1136
MalI GATC 2 cut(s) 455, 1449
MboI GATC 2 cut(s) 453, 1447
MboII GAAGA 8 cut(s) 8, 448, 646, 727, 1133, 1162, 1254, 1275
MflI RGATCY 1 cut(s) 453
MhlI GDGCHC 2 cut(s) 387, 486
MlsI TGGCCA 1 cut(s) 750
MluNI TGGCCA 1 cut(s) 750
MlyI GAGTC 3 cut(s) 540, 543, 830
MmeI TCCRAC 2 cut(s) 185, 459
Mox20I TGGCCA 1 cut(s) 750
Mph1103I ATGCAT 2 cut(s) 191, 645
MscI TGGCCA 1 cut(s) 750
MseI TTAA 2 cut(s) 150, 603
MslI CAYNNNNRTG 2 cut(s) 644, 1134
Msp20I TGGCCA 1 cut(s) 750
MspA1I CMGCKG 1 cut(s) 560
MspI CCGG 1 cut(s) 372
MspR9I CCNGG 5 cut(s) 303, 360, 522, 576, 1296
Mva1269I GAATGC 1 cut(s) 1162
MvaI CCWGG 5 cut(s) 303, 360, 522, 576, 1296
MvnI CGCG 1 cut(s) 321
MwoI GCNNNNNNNGC 3 cut(s) 28, 33, 1395
NdeI CATATG 1 cut(s) 645
NdeII GATC 2 cut(s) 453, 1447
NlaIV GGNNCC 8 cut(s) 217, 291, 357, 516, 695, 771, 981, 1467
NmuCI GTSAC 2 cut(s) 822, 1136
NsiI ATGCAT 2 cut(s) 191, 645
NspI RCATGY 1 cut(s) 643
NspV TTCGAA 1 cut(s) 120
PaeR7I CTCGAG 1 cut(s) 1568
PagI TCATGA 3 cut(s) 807, 885, 1300
PceI AGGCCT 2 cut(s) 400, 466
PctI GAATGC 1 cut(s) 1162
PfeI GAWTC 6 cut(s) 296, 688, 995, 1055, 1265, 1328
PflMI CCANNNNNTGG 1 cut(s) 1082
PfoI TCCNGGA 1 cut(s) 520
PinAI ACCGGT 1 cut(s) 371
PkrI GCNGC 4 cut(s) 7, 556, 559, 652
PleI GAGTC 3 cut(s) 539, 543, 829
PpsI GAGTC 3 cut(s) 539, 543, 829
Psp124BI GAGCTC 1 cut(s) 387
Psp6I CCWGG 5 cut(s) 301, 358, 520, 574, 1294
PspFI CCCAGC 1 cut(s) 1050
PspGI CCWGG 5 cut(s) 301, 358, 520, 574, 1294
PspN4I GGNNCC 8 cut(s) 217, 291, 357, 516, 695, 771, 981, 1467
PspPI GGNCC 7 cut(s) 216, 355, 514, 694, 851, 896, 1557
PstI CTGCAG 1 cut(s) 559
PsuI RGATCY 1 cut(s) 453
PvuII CAGCTG 1 cut(s) 560
RsaI GTAC 2 cut(s) 226, 1553
RsaNI GTAC 2 cut(s) 225, 1552
RseI CAYNNNNRTG 2 cut(s) 644, 1134
SacI GAGCTC 1 cut(s) 387
SaqAI TTAA 2 cut(s) 150, 603
SatI GCNGC 4 cut(s) 6, 555, 558, 651
Sau3AI GATC 2 cut(s) 453, 1447
Sau96I GGNCC 7 cut(s) 216, 355, 514, 694, 851, 896, 1557
ScaI AGTACT 1 cut(s) 1553
SchI GAGTC 3 cut(s) 540, 543, 830
ScrFI CCNGG 5 cut(s) 303, 360, 522, 576, 1296
SduI GDGCHC 2 cut(s) 387, 486
SfaNI GCATC 2 cut(s) 176, 1467
SfcI CTRYAG 1 cut(s) 555
Sfr274I CTCGAG 1 cut(s) 1568
SfuI TTCGAA 1 cut(s) 120
SinI GGWCC 7 cut(s) 216, 355, 514, 694, 851, 896, 1557
SlaI CTCGAG 1 cut(s) 1568
SmiMI CAYNNNNRTG 2 cut(s) 644, 1134
SmlI CTYRAG 1 cut(s) 1568
SmoI CTYRAG 1 cut(s) 1568
SseBI AGGCCT 2 cut(s) 400, 466
SsiI CCGC 2 cut(s) 335, 1036
SspMI CTAG 1 cut(s) 380
SstI GAGCTC 1 cut(s) 387
StuI AGGCCT 2 cut(s) 400, 466
StyD4I CCNGG 5 cut(s) 301, 358, 520, 574, 1294
StyI CCWWGG 1 cut(s) 90
TaaI ACNGT 2 cut(s) 200, 1432
TaiI ACGT 1 cut(s) 1030
TaqI TCGA 3 cut(s) 120, 529, 1569
TaqII GACCGA 1 cut(s) 884
TatI WGTACW 2 cut(s) 224, 1551
TfiI GAWTC 6 cut(s) 296, 688, 995, 1055, 1265, 1328
Tru1I TTAA 2 cut(s) 150, 603
Tru9I TTAA 2 cut(s) 150, 603
TscAI CASTG 3 cut(s) 498, 688, 1174
TseFI GTSAC 2 cut(s) 822, 1136
TseI GCWGC 4 cut(s) 5, 554, 557, 650
Tsp45I GTSAC 2 cut(s) 822, 1136
TspDTI ATGAA 8 cut(s) 141, 179, 516, 642, 796, 824, 902, 1247
TspRI CASTG 3 cut(s) 498, 688, 1174
Van91I CCANNNNNTGG 1 cut(s) 1082
VpaK11BI GGWCC 7 cut(s) 216, 355, 514, 694, 851, 896, 1557
XagI CCTNNNNNAGG 1 cut(s) 837
XapI RAATTY 3 cut(s) 525, 607, 1002
XceI RCATGY 1 cut(s) 643
XhoI CTCGAG 1 cut(s) 1568
XspI CTAG 1 cut(s) 380
ZrmI AGTACT 1 cut(s) 1553
Zsp2I ATGCAT 2 cut(s) 191, 645
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.