RLG00000035014

Phospholipid diacylglycerol acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0011775
Physical Location & Seq
Forward (+)
59169406 .. 59173676
4271 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035014

Sequence Viewer

Length: 2064 bp
ATGGCTGCTTCAATTCTTCGGCTTCGCAAGCTGTGCTATGTTCAGCCTGTGAGGTTTTCGGCATTGTGCTTTCAGTCCTTTGAAACAGCCGAGGACGAGGGTGCTGATGAAACCCCGAAGTTCGAAAATGAAGTGAAAGAGAAACAAAGGATTGAGAACAACAACAAGAGGCAACCGAGGGAATGGAGATGCATACATAACTGTTGTTGGATAATTGGGTCCATATGTACAACCTGGTGGCTTCTCTTGTTTTTGTACAATACCTTGCCAACTGCATTGACTGGTTTTCAGGTGCCAGAATCACCTGGAACAAGGCTTAGGCGCGAAGGCGTAAGCCCGCTTCACCCGGTTGTTATGGTCCCTGGCATTGTAACCGGTGGCCTAGAGCTGTGGGAGGGCAGGCCTTGTGCTGAAGGTCTTTTTAGGAAGCGACTTTGGGGTGGTAGTTTCTCAGAGATCTTCAAAAGGCCTATGTGTTTGTTGGAGCACCTATCACTGGACAATGAAACAGGGCTGGACCCTCCAGGAATTCGAGTCAGAGCAGTTCGGGGACTCGCTGCAGCTGACTATTTCGCTCCTGGCTACTTTGTTTGGGCTGTCCTCATTAAGAATTTGGCAAAAATTGGTTATGAAGGGAAGAACATGCATATGGCTGCCTATGATTGGAGGCTTTCTTTCCAGAACACTGAGATTCGGGACCAAGCTCTTAGTAGGTTGAAGAGTAAAATTGAGCTTATGTATGCAATCAATGGCCATAAGAAAGTGGTCGTGGTGCCTCATTCTATGGGGGCTATTTATTTTCTCCACTTCATGAAATGGGTCGAGTCACCTTCTCCTATGGGAGGTGGTGGTGGTCCAAGTTGGTGTGCCACACACATCAAAGCAATCATGAATATCGGTCCAGCATTTCTTGGTGTTCCAAGAGCAGTTAGTAATATATTTTCTGCTGAGGGTAAAGATGTTGCTTACATCAGAGCTATGGCTCCTGTTGTATTAGATTCTGAAATTCTTGGGCTTCAAACCTTTGAACATGTCATGCGGGTATCTCGGAGCTGGGATTCGTTGATTTCCTTGTTGCCAAAAGGTGGAGAAACCATTTGGGGCAACTTGGAGTGGTCTCCTGAAGACAGGCATAGCTGTGACCTGACAAAGAAGAAATATCTGCATTCCACTGCTGGTGATACCAATTACAACAACAGCAATAAGAATATAATTTTCCAAGTAAAAGAACCTATGAAGTATGGAAGAATAATCTCATTTGGCAAGGAATCATCAGAATTACCCTCTTCAGAGCTTCCTGGTCATGATATAAAGGAACTTCTTTACAGGGATTCTCCTACCAATTTCAACTCATCATGTGGAGAGGTGTGGACTGAATATGATGAGGTAAGCAGGGAAAGCATAAGAAAAATTGCAGAAAATAAGGCGTACACAGTTCAAACCCTTTTTGATCTGCTACGCTTTGTGGCACCAAAAATGATGCAACGGGCTGAGGCTCACTTCTCTCATGGGATAGCTGATAATCTTGATAATCCTAAATATGACCATTACAAGTACTGGTCCAATCCACTCGAGGCCAAGTTACCCATTGCTCCAGATTTGGAAATATACTGTTTATATGGTGTTGGACTCCCAACTGAAAGATCATACATATACAAGACATCTCCTTCTGACAAGTGCAAGAACATCCCATTCCGCATTGATAGCTCAGCAGATGGAGAGACAGGCAGCTGCCTTAAAAATGGAGCAAATTTTGTTGATGGTGATGATACTGTGCCTGTATTGAGTTCTGGATACATGTGCACCAAAGGGTGGAGAGGAAGAACCAGGTTCAACCCATCCGGTATTGCTACCTACATAAGGGAGTACCAACACAAGCGACCTGGTAGTTTTATTGAGGGAAGGGGTACGGAGAGTGGTTCACATGTTGACATCATGGGAAATGTGGCATTAATCGAAGATATCCTACGCATTGCTGCCGGAGCCACTGGTACACAGATTGGAGGTGATAGGATCTACTCAGATATTATGAGAATGTCTGAGAGAATAAATCTTCAGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 map00561
Pfam Domains
Protein Families
LCAT Lipase_GDSL

Protein Analysis

688

Amino Acids

77.34

Weight (kDa)

8.05

Isoelectric Point (pI)

48.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCAT PF02450 141 - 647 3e-65 Lecithin:cholesterol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011775)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G44830
fragaria_vesca FvH4_3g30050
malus_domestica MD11G1154200.v1.1
prunus_persica Prupe.6G116700_v2.0.a1 Prupe.6G116700_v2.0.a1
pyrus_communis pycom11g12590
rosa_chinensis RchiOBHm_Chr5g0055851 RchiOBHm_Chr5g0055861
rosa_laevigata RLG00000035014
rosa_multiflora Rmu_sc0003306.1_g000008
rosa_roxburghii Rroxscaffold_1G00024510
rosa_rugosa Rorug05G0295200 Rorug05G0295200 Rorug05G0295300
rosa_samantha Rh5AG366600 Rh5BG376800 Rh5CG399800 Rh5DG389800
rosa_wichuraiana Rw5G034360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 292, 772, 1468
AccB7I CCANNNNNTGG 2 cut(s) 1085, 1812
AccII CGCG 1 cut(s) 324
AciI CCGC 3 cut(s) 338, 1039, 1696
AclWI GGATC 1 cut(s) 2021
AcoI YGGCCR 1 cut(s) 751
AcsI RAATTY 4 cut(s) 528, 610, 1005, 1750
AcuI CTGAAG 4 cut(s) 432, 1143, 1272, 2039
AfaI GTAC 7 cut(s) 229, 257, 1430, 1556, 1868, 1909, 1993
AfiI CCNNNNNNNGG 6 cut(s) 496, 663, 842, 1085, 1812, 1860
AflIII ACRYGT 3 cut(s) 1030, 1797, 1924
AgeI ACCGGT 1 cut(s) 374
AgsI TTSAA 9 cut(s) 12, 83, 463, 718, 1019, 1028, 1348, 1439, 1834
AjnI CCWGG 8 cut(s) 233, 304, 361, 523, 577, 1297, 1826, 1882
Alw21I GWGCWC 2 cut(s) 489, 1805
Alw26I GTCTC 2 cut(s) 1122, 1715
Alw44I GTGCAC 1 cut(s) 1801
AlwI GGATC 1 cut(s) 2021
Ama87I CYCGRG 1 cut(s) 1571
AoxI GGCC 5 cut(s) 379, 401, 467, 751, 1575
ApaLI GTGCAC 1 cut(s) 1801
ApeKI GCWGC 7 cut(s) 5, 557, 560, 653, 1728, 1731, 1976
ApoI RAATTY 4 cut(s) 528, 610, 1005, 1750
AseI ATTAAT 1 cut(s) 1952
AsiGI ACCGGT 1 cut(s) 374
AspLEI GCGC 1 cut(s) 324
AspS9I GGNCC 7 cut(s) 219, 358, 517, 697, 854, 899, 1560
AsuC2I CCSGG 1 cut(s) 347
AsuHPI GGTGA 6 cut(s) 294, 335, 819, 1190, 1775, 2018
AsuII TTCGAA 1 cut(s) 123
AvaI CYCGRG 1 cut(s) 1571
AvaII GGWCC 7 cut(s) 219, 358, 517, 697, 854, 899, 1560
BaeGI GKGCMC 1 cut(s) 1805
BalI TGGCCA 1 cut(s) 753
BanI GGYRCC 3 cut(s) 292, 772, 1468
BarI GAAGNNNNNNTAC 4 cut(s) 324, 356, 1950, 1982
BbsI GAAGAC 1 cut(s) 1131
Bbv12I GWGCWC 2 cut(s) 489, 1805
BbvCI CCTCAGC 2 cut(s) 948, 1491
BbvI GCAGC 6 cut(s) 544, 572, 640, 1718, 1740, 1963
BccI CCATC 3 cut(s) 1709, 1754, 1846
BciT130I CCWGG 8 cut(s) 235, 306, 363, 525, 579, 1299, 1828, 1884
BciVI GTATCC 1 cut(s) 1787
BcnI CCSGG 1 cut(s) 347
BcoDI GTCTC 2 cut(s) 1122, 1715
BfaI CTAG 1 cut(s) 383
BfmI CTRYAG 1 cut(s) 558
BfuI GTATCC 1 cut(s) 1787
BglII AGATCT 1 cut(s) 456
BisI GCNGC 7 cut(s) 6, 558, 561, 654, 1729, 1732, 1977
BlpI GCTNAGC 1 cut(s) 1708
BlsI GCNGC 7 cut(s) 7, 559, 562, 655, 1730, 1733, 1978
BmcAI AGTACT 1 cut(s) 1556
Bme1390I CCNGG 9 cut(s) 235, 306, 347, 363, 525, 579, 1299, 1828, 1884
Bme18I GGWCC 7 cut(s) 219, 358, 517, 697, 854, 899, 1560
BmeT110I CYCGRG 1 cut(s) 1571
BmgT120I GGNCC 7 cut(s) 219, 358, 517, 697, 854, 899, 1560
BmiI GGNNCC 9 cut(s) 220, 294, 360, 519, 698, 774, 984, 1470, 1984
BmrFI CCNGG 9 cut(s) 235, 306, 347, 363, 525, 579, 1299, 1828, 1884
BmsI GCATC 2 cut(s) 179, 1470
BpiI GAAGAC 1 cut(s) 1131
BpmI CTGGAG 2 cut(s) 507, 1578
Bpu10I CCTNAGC 3 cut(s) 317, 948, 1491
Bpu1102I GCTNAGC 1 cut(s) 1708
Bpu14I TTCGAA 1 cut(s) 123
BpuMI CCSGG 1 cut(s) 347
BsaI GGTCTC 1 cut(s) 1122
BsaJI CCNNGG 3 cut(s) 90, 176, 361
BsaWI WCCGGW 2 cut(s) 374, 1841
Bsc4I CCNNNNNNNGG 6 cut(s) 496, 663, 842, 1085, 1812, 1860
Bse118I RCCGGY 1 cut(s) 374
Bse1I ACTGG 4 cut(s) 286, 501, 1562, 1993
Bse3DI GCAATG 2 cut(s) 1587, 1971
BseBI CCWGG 8 cut(s) 235, 306, 363, 525, 579, 1299, 1828, 1884
BseDI CCNNGG 3 cut(s) 90, 176, 361
BseGI GGATG 2 cut(s) 1686, 1838
BseLI CCNNNNNNNGG 6 cut(s) 496, 663, 842, 1085, 1812, 1860
BseMI GCAATG 2 cut(s) 1587, 1971
BseMII CTCAG 7 cut(s) 465, 678, 939, 1482, 1722, 2031, 2034
BseNI ACTGG 4 cut(s) 286, 501, 1562, 1993
BseSI GKGCMC 1 cut(s) 1805
BseXI GCAGC 6 cut(s) 544, 572, 640, 1718, 1740, 1963
BseYI CCCAGC 1 cut(s) 1053
Bsh1236I CGCG 1 cut(s) 324
BshFI GGCC 5 cut(s) 381, 403, 469, 753, 1577
BshNI GGYRCC 3 cut(s) 292, 772, 1468
BshTI ACCGGT 1 cut(s) 374
BsiHKAI GWGCWC 2 cut(s) 489, 1805
BsiHKCI CYCGRG 1 cut(s) 1571
BsiSI CCGG 4 cut(s) 347, 375, 1842, 1980
BslFI GGGAC 3 cut(s) 344, 564, 710
BslI CCNNNNNNNGG 6 cut(s) 496, 663, 842, 1085, 1812, 1860
BsmAI GTCTC 2 cut(s) 1122, 1715
BsmFI GGGAC 3 cut(s) 344, 564, 710
BsmI GAATGC 1 cut(s) 1165
BsnI GGCC 5 cut(s) 381, 403, 469, 753, 1577
Bso31I GGTCTC 1 cut(s) 1122
BsoBI CYCGRG 1 cut(s) 1571
Bsp119I TTCGAA 1 cut(s) 123
Bsp1286I GDGCHC 2 cut(s) 489, 1805
Bsp1407I TGTACA 2 cut(s) 227, 255
Bsp143I GATC 4 cut(s) 456, 1450, 1643, 2013
Bsp1720I GCTNAGC 1 cut(s) 1708
BspACI CCGC 3 cut(s) 338, 1039, 1696
BspANI GGCC 5 cut(s) 381, 403, 469, 753, 1577
BspCNI CTCAG 7 cut(s) 464, 679, 940, 1483, 1721, 2032, 2033
BspFNI CGCG 1 cut(s) 324
BspHI TCATGA 3 cut(s) 810, 888, 1303
BspLI GGNNCC 9 cut(s) 220, 294, 360, 519, 698, 774, 984, 1470, 1984
BspMAI CTGCAG 1 cut(s) 562
BspPI GGATC 1 cut(s) 2021
BspT104I TTCGAA 1 cut(s) 123
BspT107I GGYRCC 3 cut(s) 292, 772, 1468
BspTNI GGTCTC 1 cut(s) 1122
BsrDI GCAATG 2 cut(s) 1587, 1971
BsrFI RCCGGY 1 cut(s) 374
BsrGI TGTACA 2 cut(s) 227, 255
BsrI ACTGG 4 cut(s) 286, 501, 1562, 1993
BssAI RCCGGY 1 cut(s) 374
BssECI CCNNGG 3 cut(s) 90, 176, 361
BssMI GATC 4 cut(s) 456, 1450, 1643, 2013
Bst2UI CCWGG 8 cut(s) 235, 306, 363, 525, 579, 1299, 1828, 1884
Bst4CI ACNGT 4 cut(s) 203, 1435, 1613, 1774
Bst6I CTCTTC 2 cut(s) 713, 1291
BstAPI GCANNNNNTGC 1 cut(s) 33
BstAUI TGTACA 2 cut(s) 227, 255
BstBI TTCGAA 1 cut(s) 123
BstC8I GCNNGC 3 cut(s) 29, 338, 401
BstDEI CTNAG 9 cut(s) 317, 451, 687, 707, 948, 1491, 1708, 2020, 2040
BstENI CCTNNNNNAGG 2 cut(s) 840, 1858
BstF5I GGATG 2 cut(s) 1686, 1838
BstFNI CGCG 1 cut(s) 324
BstHHI GCGC 1 cut(s) 324
BstKTI GATC 4 cut(s) 459, 1453, 1646, 2016
BstMAI GTCTC 2 cut(s) 1122, 1715
BstMBI GATC 4 cut(s) 456, 1450, 1643, 2013
BstMWI GCNNNNNNNGC 5 cut(s) 28, 33, 1398, 1704, 1982
BstNI CCWGG 8 cut(s) 235, 306, 363, 525, 579, 1299, 1828, 1884
BstNSI RCATGY 4 cut(s) 646, 1034, 1801, 1928
BstSCI CCNGG 9 cut(s) 233, 304, 345, 361, 523, 577, 1297, 1826, 1882
BstSFI CTRYAG 1 cut(s) 558
BstSLI GKGCMC 1 cut(s) 1805
BstUI CGCG 1 cut(s) 324
BstV1I GCAGC 6 cut(s) 544, 572, 640, 1718, 1740, 1963
BstV2I GAAGAC 1 cut(s) 1131
BstX2I RGATCY 2 cut(s) 456, 2013
BstYI RGATCY 2 cut(s) 456, 2013
BsuI GTATCC 1 cut(s) 1787
BsuRI GGCC 5 cut(s) 381, 403, 469, 753, 1577
BtsCI GGATG 2 cut(s) 1686, 1838
BtsI GCAGTG 1 cut(s) 1170
BtsIMutI CAGTG 4 cut(s) 494, 684, 1170, 1986
Cac8I GCNNGC 3 cut(s) 29, 338, 401
CciI TCATGA 3 cut(s) 810, 888, 1303
CfoI GCGC 1 cut(s) 324
Cfr10I RCCGGY 1 cut(s) 374
Cfr13I GGNCC 7 cut(s) 219, 358, 517, 697, 854, 899, 1560
CsiI ACCWGGT 3 cut(s) 233, 1826, 1882
Csp6I GTAC 7 cut(s) 228, 256, 1429, 1555, 1867, 1908, 1992
CspAI ACCGGT 1 cut(s) 374
CviQI GTAC 7 cut(s) 228, 256, 1429, 1555, 1867, 1908, 1992
DdeI CTNAG 9 cut(s) 317, 451, 687, 707, 948, 1491, 1708, 2020, 2040
DpnI GATC 4 cut(s) 458, 1452, 1645, 2015
DpnII GATC 4 cut(s) 456, 1450, 1643, 2013
EaeI YGGCCR 1 cut(s) 751
Eam1104I CTCTTC 2 cut(s) 713, 1291
EarI CTCTTC 2 cut(s) 713, 1291
Eco147I AGGCCT 2 cut(s) 403, 469
Eco31I GGTCTC 1 cut(s) 1122
Eco32I GATATC 1 cut(s) 1963
Eco47I GGWCC 7 cut(s) 219, 358, 517, 697, 854, 899, 1560
Eco57I CTGAAG 4 cut(s) 432, 1143, 1272, 2039
Eco88I CYCGRG 1 cut(s) 1571
EcoNI CCTNNNNNAGG 2 cut(s) 840, 1858
EcoRI GAATTC 1 cut(s) 528
EcoRII CCWGG 8 cut(s) 233, 304, 361, 523, 577, 1297, 1826, 1882
EcoRV GATATC 1 cut(s) 1963
EcoT22I ATGCAT 2 cut(s) 194, 648
FaqI GGGAC 3 cut(s) 344, 564, 710
FauI CCCGC 2 cut(s) 345, 1032
FauNDI CATATG 2 cut(s) 224, 648
Fnu4HI GCNGC 7 cut(s) 6, 558, 561, 654, 1729, 1732, 1977
FokI GGATG 2 cut(s) 1673, 1825
Fsp4HI GCNGC 7 cut(s) 6, 558, 561, 654, 1729, 1732, 1977
FspBI CTAG 1 cut(s) 383
GlaI GCGC 1 cut(s) 323
GluI GCNGC 7 cut(s) 6, 558, 561, 654, 1729, 1732, 1977
GsaI CCCAGC 1 cut(s) 1057
GsuI CTGGAG 2 cut(s) 507, 1578
HaeIII GGCC 5 cut(s) 381, 403, 469, 753, 1577
HapII CCGG 4 cut(s) 347, 375, 1842, 1980
HhaI GCGC 1 cut(s) 324
Hin6I GCGC 1 cut(s) 322
HinP1I GCGC 1 cut(s) 322
HincII GTYRAC 1 cut(s) 1930
HindII GTYRAC 1 cut(s) 1930
HpaII CCGG 4 cut(s) 347, 375, 1842, 1980
HphI GGTGA 6 cut(s) 294, 335, 819, 1190, 1775, 2018
Hpy166II GTNNAC 6 cut(s) 1371, 1431, 1803, 1922, 1930, 1994
Hpy188III TCNNGA 9 cut(s) 679, 695, 811, 889, 1121, 1304, 1526, 1595, 1791
Hpy8I GTNNAC 6 cut(s) 1371, 1431, 1803, 1922, 1930, 1994
HpyAV CCTTC 6 cut(s) 320, 407, 626, 840, 1677, 1896
HpyCH4III ACNGT 4 cut(s) 203, 1435, 1613, 1774
HpyF10VI GCNNNNNNNGC 5 cut(s) 28, 33, 1398, 1704, 1982
HpyF3I CTNAG 9 cut(s) 317, 451, 687, 707, 948, 1491, 1708, 2020, 2040
HspAI GCGC 1 cut(s) 322
Kzo9I GATC 4 cut(s) 456, 1450, 1643, 2013
LmnI GCTCC 7 cut(s) 484, 580, 988, 1050, 1597, 1745, 1982
Lsp1109I GCAGC 6 cut(s) 544, 572, 640, 1718, 1740, 1963
LweI GCATC 2 cut(s) 179, 1470
MabI ACCWGGT 3 cut(s) 233, 1826, 1882
MaeI CTAG 1 cut(s) 383
MaeIII GTNAC 4 cut(s) 370, 825, 1139, 1581
MalI GATC 4 cut(s) 458, 1452, 1645, 2015
MboI GATC 4 cut(s) 456, 1450, 1643, 2013
MflI RGATCY 2 cut(s) 456, 2013
MhlI GDGCHC 2 cut(s) 489, 1805
MlsI TGGCCA 1 cut(s) 753
MluNI TGGCCA 1 cut(s) 753
MlyI GAGTC 4 cut(s) 543, 546, 833, 1623
MmeI TCCRAC 3 cut(s) 188, 462, 1606
Mox20I TGGCCA 1 cut(s) 753
Mph1103I ATGCAT 2 cut(s) 194, 648
MscI TGGCCA 1 cut(s) 753
MseI TTAA 3 cut(s) 606, 1737, 1952
MslI CAYNNNNRTG 2 cut(s) 647, 1137
Msp20I TGGCCA 1 cut(s) 753
MspA1I CMGCKG 3 cut(s) 563, 1731, 2059
MspI CCGG 4 cut(s) 347, 375, 1842, 1980
MspR9I CCNGG 9 cut(s) 235, 306, 347, 363, 525, 579, 1299, 1828, 1884
Mva1269I GAATGC 1 cut(s) 1165
MvaI CCWGG 8 cut(s) 235, 306, 363, 525, 579, 1299, 1828, 1884
MvnI CGCG 1 cut(s) 324
MwoI GCNNNNNNNGC 5 cut(s) 28, 33, 1398, 1704, 1982
NciI CCSGG 1 cut(s) 347
NdeI CATATG 2 cut(s) 224, 648
NdeII GATC 4 cut(s) 456, 1450, 1643, 2013
NlaIV GGNNCC 9 cut(s) 220, 294, 360, 519, 698, 774, 984, 1470, 1984
NmeAIII GCCGAG 1 cut(s) 115
NmuCI GTSAC 2 cut(s) 825, 1139
NsiI ATGCAT 2 cut(s) 194, 648
NspI RCATGY 4 cut(s) 646, 1034, 1801, 1928
NspV TTCGAA 1 cut(s) 123
PaeR7I CTCGAG 1 cut(s) 1571
PagI TCATGA 3 cut(s) 810, 888, 1303
PceI AGGCCT 2 cut(s) 403, 469
PciI ACATGT 3 cut(s) 1030, 1797, 1924
PctI GAATGC 1 cut(s) 1165
PfeI GAWTC 6 cut(s) 299, 691, 998, 1058, 1268, 1331
PflMI CCANNNNNTGG 2 cut(s) 1085, 1812
PfoI TCCNGGA 1 cut(s) 523
PinAI ACCGGT 1 cut(s) 374
PkrI GCNGC 7 cut(s) 7, 559, 562, 655, 1730, 1733, 1978
PleI GAGTC 4 cut(s) 542, 546, 832, 1623
PpsI GAGTC 4 cut(s) 542, 546, 832, 1623
PscI ACATGT 3 cut(s) 1030, 1797, 1924
PshBI ATTAAT 1 cut(s) 1952
Psp6I CCWGG 8 cut(s) 233, 304, 361, 523, 577, 1297, 1826, 1882
PspFI CCCAGC 1 cut(s) 1053
PspGI CCWGG 8 cut(s) 233, 304, 361, 523, 577, 1297, 1826, 1882
PspN4I GGNNCC 9 cut(s) 220, 294, 360, 519, 698, 774, 984, 1470, 1984
PspPI GGNCC 7 cut(s) 219, 358, 517, 697, 854, 899, 1560
PspXI VCTCGAGB 1 cut(s) 1571
PstI CTGCAG 1 cut(s) 562
PsuI RGATCY 2 cut(s) 456, 2013
PvuII CAGCTG 3 cut(s) 563, 1731, 2059
RsaI GTAC 7 cut(s) 229, 257, 1430, 1556, 1868, 1909, 1993
RsaNI GTAC 7 cut(s) 228, 256, 1429, 1555, 1867, 1908, 1992
RseI CAYNNNNRTG 2 cut(s) 647, 1137
SaqAI TTAA 3 cut(s) 606, 1737, 1952
SatI GCNGC 7 cut(s) 6, 558, 561, 654, 1729, 1732, 1977
Sau3AI GATC 4 cut(s) 456, 1450, 1643, 2013
Sau96I GGNCC 7 cut(s) 219, 358, 517, 697, 854, 899, 1560
ScaI AGTACT 1 cut(s) 1556
SchI GAGTC 4 cut(s) 543, 546, 833, 1623
ScrFI CCNGG 9 cut(s) 235, 306, 347, 363, 525, 579, 1299, 1828, 1884
SduI GDGCHC 2 cut(s) 489, 1805
SexAI ACCWGGT 3 cut(s) 233, 1826, 1882
SfaNI GCATC 2 cut(s) 179, 1470
SfcI CTRYAG 1 cut(s) 558
Sfr274I CTCGAG 1 cut(s) 1571
SfuI TTCGAA 1 cut(s) 123
SinI GGWCC 7 cut(s) 219, 358, 517, 697, 854, 899, 1560
SlaI CTCGAG 1 cut(s) 1571
SmiMI CAYNNNNRTG 2 cut(s) 647, 1137
SmlI CTYRAG 1 cut(s) 1571
SmoI CTYRAG 1 cut(s) 1571
SseBI AGGCCT 2 cut(s) 403, 469
SsiI CCGC 3 cut(s) 338, 1039, 1696
SspMI CTAG 1 cut(s) 383
StuI AGGCCT 2 cut(s) 403, 469
StyD4I CCNGG 9 cut(s) 233, 304, 345, 361, 523, 577, 1297, 1826, 1882
TaaI ACNGT 4 cut(s) 203, 1435, 1613, 1774
TaqI TCGA 5 cut(s) 123, 532, 822, 1572, 1956
TaqII GACCGA 1 cut(s) 887
TatI WGTACW 3 cut(s) 227, 255, 1554
TfiI GAWTC 6 cut(s) 299, 691, 998, 1058, 1268, 1331
Tru1I TTAA 3 cut(s) 606, 1737, 1952
Tru9I TTAA 3 cut(s) 606, 1737, 1952
TscAI CASTG 4 cut(s) 501, 691, 1177, 1993
TseFI GTSAC 2 cut(s) 825, 1139
TseI GCWGC 7 cut(s) 5, 557, 560, 653, 1728, 1731, 1976
Tsp45I GTSAC 2 cut(s) 825, 1139
TspDTI ATGAA 8 cut(s) 123, 144, 519, 645, 799, 827, 905, 1250
TspGWI ACGGA 1 cut(s) 1925
TspRI CASTG 4 cut(s) 501, 691, 1177, 1993
Van91I CCANNNNNTGG 2 cut(s) 1085, 1812
VneI GTGCAC 1 cut(s) 1801
VpaK11BI GGWCC 7 cut(s) 219, 358, 517, 697, 854, 899, 1560
VspI ATTAAT 1 cut(s) 1952
XagI CCTNNNNNAGG 2 cut(s) 840, 1858
XapI RAATTY 4 cut(s) 528, 610, 1005, 1750
XceI RCATGY 4 cut(s) 646, 1034, 1801, 1928
XhoI CTCGAG 1 cut(s) 1571
XspI CTAG 1 cut(s) 383
ZrmI AGTACT 1 cut(s) 1556
Zsp2I ATGCAT 2 cut(s) 194, 648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.