Rw5G034360

Phospholipid diacylglycerol acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0011775
Physical Location & Seq
Forward (+)
59227026 .. 59231548
4523 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G034360.1

Sequence Viewer

Length: 2061 bp
ATGGCTGCTTCAATTCTTCGGCTTCGCAAGCTGTGCTATGTTCAGCCTGTGAGGTTTTCGGCATTGTGCTTTCAGTCCTTTGAAACAGCCAAGGACGAGGGTGCTGAAACTCCGAAGTTCGAAAATGAAGTGAAAGAGAAACAGAGGATTAAGAACAAGAACAAGAGGCAACCGAGGGAATGGAGATGCATACATAACTGTTGTTGGATAATTGGGTCCATGTGTACAACTTGGTGGCTTCTCTTGTTTTTGTACAACACCTTGCCAACTGCATTGACTGGTTTTCAGGTGCCAGAATCACCTGGAACAAGGCTTAGGCGCGAAGGTGTAAGCCTGCTTCACCCAGTTGTCATGGTCCCTGGCATTGTAACCGGTGGCCTAGAGCTCTGGGAGGGTAGGCCTTGTGCTGAAGGTCTTTTTAGGAAGCGACTTTGGGGTGGTAGTTTCTCAGAGATCTTCAAAAGGCCTATGTGTTTGTTGGAGCACCTATCACTGGACAATGAAACAGGGCTGGACCCTCCAGGAATTCGAGTCAGAGCAGTTCGGGGACTCGCTGCAGCTGACTATTTTGCTCCTGGCTACTTTGTTTGGGCTGTCCTCATTAAGAATTTGGCAAAAATTGGTTATGAAGGGAAGAACATGCATATGGCTGCCTATGATTGGAGGCTTTCTTTCCAGAACACTGAGATTCGGGACCAAGCTCTTAGTAGGTTGAAGAGTAAAATTGAGCTTATGTATGCAATCAATGGCCATAAGAAAGTGGTCGTGGTGCCTCATTCTATGGGGGCTATTTATTTTCTCCACTTCATGAAATGGGTCGAGTCACCTCCTCCTATGGGAGGTGGTGGTGGTCCAAGTTGGTGTGCCACGCACATCAAAGCAATCATGAATATCGGTCCAGCATTTCTTGGTGTTCCAAGAGCAGTTAGTAATATATTTTCTGCTGAGGGTAAAGATGTTGCTTACATCAGAGCTATGGCTCCTGTTGTTTTAGATTCTGAAATTCTTGGGCTTCAAACCTTTGAACATGTCATGCGGGTATCTCGGAGCTGGGATTCCTTGATTTCCTTGTTGCCAAAAGGTGGAGAAACCATTTGGGGCAACTTGGAGTGGTCTCCTGAAGACGGGCATAGCTGTGACCTGACAAAGAAGAAATATCTGCATTCCACTGCTGGTGATACCAATTACAACGACAGCAATAAGAATATAATTTTCCAAGTAAAAGAACCTATGAAGTATGGAAGAATAATCTCATTTGGCAAGGAATCATCAGAATTACCCTCTTCAGAGCTTCCTGGTCATGATATAAAGGAACTTCTTTACAGGGATTCTCCTACCAATTTCAACTCATCATGTGGAGAGGTGTGGACTGAATATGATGAGGTCAGCAGGGAAAGCATAAGAAAAATTGCAGAAAATAAGGCTTACACAGTTCAAACCCTTTTTGATCTGCTACGCTTTGTGGCACCAAAAATGATGCAACGGGCTGAGGCTCACTTCTCTCATGGGATAGCTGATAATCTTGATAATCCTAAATATGACCATTACAAGTACTGGTCCAATCCACTCGAGACCAAGTTACCCATTGCTCCAGATTTGGAAATATACTGTTTATATGGTGTTGGAATCCCAACTGAAAGATCATACATATACAAGACATCTCCTTCTGACAAGTGCAAGAACATCCCATTCCGCATTGATAGCTCAGCAGATGGAGAGACAGGCAGCTGCCTTAAAAATGGAGCACATTTTGTTGATGGTGATGATACCGTGCCTGTATTGAGTGCTGGATACATGTGCACCAAAGGGTGGAGAGGAAGAACCAGGTTCAACCCATCCGGTATTGCTACCTACATAAGGGAGTACCAGCACAAGCGACCTGGTAGTTTTATTGAGGGAAGGGGTACGGAGAGTGGTTCACATGTTGACATCATGGGAAATGTGGCATTAATCGAAGATATCCTATGCATTGCTGCCGGAGCCACTGTTGCACAGATTGGAGGTGATAGGATCTACTCAGATATTATGAGAATGTCTGAGAGAATAAATCTAAAGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 map00561
Pfam Domains
Protein Families
LCAT Lipase_GDSL

Protein Analysis

686

Amino Acids

77.18

Weight (kDa)

8.51

Isoelectric Point (pI)

45.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCAT PF02450 140 - 646 6e-65 Lecithin:cholesterol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011775)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G44830
fragaria_vesca FvH4_3g30050
malus_domestica MD11G1154200.v1.1
prunus_persica Prupe.6G116700_v2.0.a1 Prupe.6G116700_v2.0.a1
pyrus_communis pycom11g12590
rosa_chinensis RchiOBHm_Chr5g0055851 RchiOBHm_Chr5g0055861
rosa_laevigata RLG00000035014
rosa_multiflora Rmu_sc0003306.1_g000008
rosa_roxburghii Rroxscaffold_1G00024510
rosa_rugosa Rorug05G0295200 Rorug05G0295200 Rorug05G0295300
rosa_samantha Rh5AG366600 Rh5BG376800 Rh5CG399800 Rh5DG389800
rosa_wichuraiana Rw5G034360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 289, 769, 1465
AccB7I CCANNNNNTGG 2 cut(s) 1082, 1809
AccII CGCG 1 cut(s) 321
AciI CCGC 2 cut(s) 1036, 1693
AclWI GGATC 1 cut(s) 2018
AcoI YGGCCR 1 cut(s) 748
AcsI RAATTY 3 cut(s) 525, 607, 1002
AcuI CTGAAG 3 cut(s) 429, 1140, 1269
AfaI GTAC 5 cut(s) 226, 254, 1553, 1865, 1906
AfiI CCNNNNNNNGG 8 cut(s) 493, 660, 836, 839, 1082, 1124, 1809, 1857
AflIII ACRYGT 3 cut(s) 1027, 1794, 1921
AgeI ACCGGT 1 cut(s) 371
AgsI TTSAA 9 cut(s) 12, 83, 460, 715, 1016, 1025, 1345, 1436, 1831
AjnI CCWGG 7 cut(s) 301, 358, 520, 574, 1294, 1823, 1879
Alw21I GWGCWC 4 cut(s) 387, 486, 1747, 1802
Alw26I GTCTC 3 cut(s) 1119, 1565, 1712
Alw44I GTGCAC 1 cut(s) 1798
AlwI GGATC 1 cut(s) 2018
Ama87I CYCGRG 1 cut(s) 1568
AoxI GGCC 4 cut(s) 376, 398, 464, 748
ApaLI GTGCAC 1 cut(s) 1798
ApeKI GCWGC 7 cut(s) 5, 554, 557, 650, 1725, 1728, 1973
ApoI RAATTY 3 cut(s) 525, 607, 1002
AseI ATTAAT 1 cut(s) 1949
AsiGI ACCGGT 1 cut(s) 371
AspLEI GCGC 1 cut(s) 321
AspS9I GGNCC 7 cut(s) 216, 355, 514, 694, 851, 896, 1557
AsuHPI GGTGA 6 cut(s) 291, 332, 816, 1187, 1772, 2015
AsuII TTCGAA 1 cut(s) 120
AvaI CYCGRG 1 cut(s) 1568
AvaII GGWCC 7 cut(s) 216, 355, 514, 694, 851, 896, 1557
BaeGI GKGCMC 1 cut(s) 1802
BalI TGGCCA 1 cut(s) 750
BanI GGYRCC 3 cut(s) 289, 769, 1465
BanII GRGCYC 1 cut(s) 387
BarI GAAGNNNNNNTAC 2 cut(s) 321, 353
BbsI GAAGAC 1 cut(s) 1128
Bbv12I GWGCWC 4 cut(s) 387, 486, 1747, 1802
BbvCI CCTCAGC 2 cut(s) 945, 1488
BbvI GCAGC 6 cut(s) 541, 569, 637, 1715, 1737, 1960
BccI CCATC 3 cut(s) 1706, 1751, 1843
BciT130I CCWGG 7 cut(s) 303, 360, 522, 576, 1296, 1825, 1881
BciVI GTATCC 1 cut(s) 1784
BcoDI GTCTC 3 cut(s) 1119, 1565, 1712
BfaI CTAG 1 cut(s) 380
BfmI CTRYAG 1 cut(s) 555
BfuI GTATCC 1 cut(s) 1784
BglII AGATCT 1 cut(s) 453
BisI GCNGC 7 cut(s) 6, 555, 558, 651, 1726, 1729, 1974
BlpI GCTNAGC 1 cut(s) 1705
BlsI GCNGC 7 cut(s) 7, 556, 559, 652, 1727, 1730, 1975
BmcAI AGTACT 1 cut(s) 1553
Bme1390I CCNGG 7 cut(s) 303, 360, 522, 576, 1296, 1825, 1881
Bme18I GGWCC 7 cut(s) 216, 355, 514, 694, 851, 896, 1557
BmeT110I CYCGRG 1 cut(s) 1568
BmgT120I GGNCC 7 cut(s) 216, 355, 514, 694, 851, 896, 1557
BmiI GGNNCC 9 cut(s) 217, 291, 357, 516, 695, 771, 981, 1467, 1981
BmrFI CCNGG 7 cut(s) 303, 360, 522, 576, 1296, 1825, 1881
BmrI ACTGGG 1 cut(s) 338
BmsI GCATC 2 cut(s) 176, 1467
BmuI ACTGGG 1 cut(s) 338
BpiI GAAGAC 1 cut(s) 1128
BpmI CTGGAG 2 cut(s) 504, 1575
Bpu10I CCTNAGC 3 cut(s) 314, 945, 1488
Bpu1102I GCTNAGC 1 cut(s) 1705
Bpu14I TTCGAA 1 cut(s) 120
BsaI GGTCTC 2 cut(s) 1119, 1565
BsaJI CCNNGG 3 cut(s) 90, 173, 358
BsaWI WCCGGW 2 cut(s) 371, 1838
BsaXI ACNNNNNCTCC 2 cut(s) 1971, 2001
Bsc4I CCNNNNNNNGG 8 cut(s) 493, 660, 836, 839, 1082, 1124, 1809, 1857
Bse118I RCCGGY 1 cut(s) 371
Bse1I ACTGG 4 cut(s) 283, 344, 498, 1559
Bse3DI GCAATG 2 cut(s) 1584, 1968
BseBI CCWGG 7 cut(s) 303, 360, 522, 576, 1296, 1825, 1881
BseDI CCNNGG 3 cut(s) 90, 173, 358
BseGI GGATG 2 cut(s) 1683, 1835
BseLI CCNNNNNNNGG 8 cut(s) 493, 660, 836, 839, 1082, 1124, 1809, 1857
BseMI GCAATG 2 cut(s) 1584, 1968
BseMII CTCAG 7 cut(s) 462, 675, 936, 1479, 1719, 2028, 2031
BseNI ACTGG 4 cut(s) 283, 344, 498, 1559
BseRI GAGGAG 1 cut(s) 819
BseSI GKGCMC 1 cut(s) 1802
BseXI GCAGC 6 cut(s) 541, 569, 637, 1715, 1737, 1960
BseYI CCCAGC 1 cut(s) 1050
Bsh1236I CGCG 1 cut(s) 321
BshFI GGCC 4 cut(s) 378, 400, 466, 750
BshNI GGYRCC 3 cut(s) 289, 769, 1465
BshTI ACCGGT 1 cut(s) 371
BsiHKAI GWGCWC 4 cut(s) 387, 486, 1747, 1802
BsiHKCI CYCGRG 1 cut(s) 1568
BsiSI CCGG 3 cut(s) 372, 1839, 1977
BslFI GGGAC 3 cut(s) 341, 561, 707
BslI CCNNNNNNNGG 8 cut(s) 493, 660, 836, 839, 1082, 1124, 1809, 1857
BsmAI GTCTC 3 cut(s) 1119, 1565, 1712
BsmFI GGGAC 3 cut(s) 341, 561, 707
BsmI GAATGC 1 cut(s) 1162
BsnI GGCC 4 cut(s) 378, 400, 466, 750
Bso31I GGTCTC 2 cut(s) 1119, 1565
BsoBI CYCGRG 1 cut(s) 1568
Bsp119I TTCGAA 1 cut(s) 120
Bsp1286I GDGCHC 4 cut(s) 387, 486, 1747, 1802
Bsp1407I TGTACA 2 cut(s) 224, 252
Bsp143I GATC 4 cut(s) 453, 1447, 1640, 2010
Bsp1720I GCTNAGC 1 cut(s) 1705
BspACI CCGC 2 cut(s) 1036, 1693
BspANI GGCC 4 cut(s) 378, 400, 466, 750
BspCNI CTCAG 7 cut(s) 461, 676, 937, 1480, 1718, 2029, 2030
BspFNI CGCG 1 cut(s) 321
BspHI TCATGA 3 cut(s) 807, 885, 1300
BspLI GGNNCC 9 cut(s) 217, 291, 357, 516, 695, 771, 981, 1467, 1981
BspMAI CTGCAG 1 cut(s) 559
BspPI GGATC 1 cut(s) 2018
BspT104I TTCGAA 1 cut(s) 120
BspT107I GGYRCC 3 cut(s) 289, 769, 1465
BspTNI GGTCTC 2 cut(s) 1119, 1565
BsrDI GCAATG 2 cut(s) 1584, 1968
BsrFI RCCGGY 1 cut(s) 371
BsrGI TGTACA 2 cut(s) 224, 252
BsrI ACTGG 4 cut(s) 283, 344, 498, 1559
BssAI RCCGGY 1 cut(s) 371
BssECI CCNNGG 3 cut(s) 90, 173, 358
BssMI GATC 4 cut(s) 453, 1447, 1640, 2010
BssT1I CCWWGG 1 cut(s) 90
Bst2UI CCWGG 7 cut(s) 303, 360, 522, 576, 1296, 1825, 1881
Bst4CI ACNGT 5 cut(s) 200, 1432, 1610, 1771, 1987
Bst6I CTCTTC 2 cut(s) 710, 1288
BstAPI GCANNNNNTGC 1 cut(s) 33
BstAUI TGTACA 2 cut(s) 224, 252
BstBI TTCGAA 1 cut(s) 120
BstC8I GCNNGC 2 cut(s) 29, 335
BstDEI CTNAG 9 cut(s) 314, 448, 684, 704, 945, 1488, 1705, 2017, 2037
BstENI CCTNNNNNAGG 2 cut(s) 837, 1855
BstF5I GGATG 2 cut(s) 1683, 1835
BstFNI CGCG 1 cut(s) 321
BstHHI GCGC 1 cut(s) 321
BstKTI GATC 4 cut(s) 456, 1450, 1643, 2013
BstMAI GTCTC 3 cut(s) 1119, 1565, 1712
BstMBI GATC 4 cut(s) 453, 1447, 1640, 2010
BstMWI GCNNNNNNNGC 6 cut(s) 28, 33, 1395, 1701, 1979, 1988
BstNI CCWGG 7 cut(s) 303, 360, 522, 576, 1296, 1825, 1881
BstNSI RCATGY 4 cut(s) 643, 1031, 1798, 1925
BstSCI CCNGG 4 cut(s) 301, 358, 520, 574
BstSFI CTRYAG 1 cut(s) 555
BstSLI GKGCMC 1 cut(s) 1802
BstUI CGCG 1 cut(s) 321
BstV1I GCAGC 6 cut(s) 541, 569, 637, 1715, 1737, 1960
BstV2I GAAGAC 1 cut(s) 1128
BstX2I RGATCY 2 cut(s) 453, 2010
BstYI RGATCY 2 cut(s) 453, 2010
BsuI GTATCC 1 cut(s) 1784
BsuRI GGCC 4 cut(s) 378, 400, 466, 750
BtsCI GGATG 2 cut(s) 1683, 1835
BtsI GCAGTG 1 cut(s) 1167
BtsIMutI CAGTG 4 cut(s) 491, 681, 1167, 1983
Cac8I GCNNGC 2 cut(s) 29, 335
CciI TCATGA 3 cut(s) 807, 885, 1300
CfoI GCGC 1 cut(s) 321
Cfr10I RCCGGY 1 cut(s) 371
Cfr13I GGNCC 7 cut(s) 216, 355, 514, 694, 851, 896, 1557
CsiI ACCWGGT 2 cut(s) 1823, 1879
Csp6I GTAC 5 cut(s) 225, 253, 1552, 1864, 1905
CspAI ACCGGT 1 cut(s) 371
CviQI GTAC 5 cut(s) 225, 253, 1552, 1864, 1905
DdeI CTNAG 9 cut(s) 314, 448, 684, 704, 945, 1488, 1705, 2017, 2037
DpnI GATC 4 cut(s) 455, 1449, 1642, 2012
DpnII GATC 4 cut(s) 453, 1447, 1640, 2010
EaeI YGGCCR 1 cut(s) 748
Eam1104I CTCTTC 2 cut(s) 710, 1288
EarI CTCTTC 2 cut(s) 710, 1288
Ecl136II GAGCTC 1 cut(s) 385
Eco130I CCWWGG 1 cut(s) 90
Eco147I AGGCCT 2 cut(s) 400, 466
Eco24I GRGCYC 1 cut(s) 387
Eco31I GGTCTC 2 cut(s) 1119, 1565
Eco32I GATATC 1 cut(s) 1960
Eco47I GGWCC 7 cut(s) 216, 355, 514, 694, 851, 896, 1557
Eco53kI GAGCTC 1 cut(s) 385
Eco57I CTGAAG 3 cut(s) 429, 1140, 1269
Eco88I CYCGRG 1 cut(s) 1568
EcoICRI GAGCTC 1 cut(s) 385
EcoNI CCTNNNNNAGG 2 cut(s) 837, 1855
EcoRI GAATTC 1 cut(s) 525
EcoRII CCWGG 7 cut(s) 301, 358, 520, 574, 1294, 1823, 1879
EcoRV GATATC 1 cut(s) 1960
EcoT14I CCWWGG 1 cut(s) 90
EcoT22I ATGCAT 3 cut(s) 191, 645, 1970
EcoT38I GRGCYC 1 cut(s) 387
ErhI CCWWGG 1 cut(s) 90
FaqI GGGAC 3 cut(s) 341, 561, 707
FauI CCCGC 1 cut(s) 1029
FauNDI CATATG 1 cut(s) 645
Fnu4HI GCNGC 7 cut(s) 6, 555, 558, 651, 1726, 1729, 1974
FokI GGATG 2 cut(s) 1670, 1822
FriOI GRGCYC 1 cut(s) 387
Fsp4HI GCNGC 7 cut(s) 6, 555, 558, 651, 1726, 1729, 1974
FspBI CTAG 1 cut(s) 380
GlaI GCGC 1 cut(s) 320
GluI GCNGC 7 cut(s) 6, 555, 558, 651, 1726, 1729, 1974
GsaI CCCAGC 1 cut(s) 1054
GsuI CTGGAG 2 cut(s) 504, 1575
HaeIII GGCC 4 cut(s) 378, 400, 466, 750
HapII CCGG 3 cut(s) 372, 1839, 1977
HhaI GCGC 1 cut(s) 321
Hin6I GCGC 1 cut(s) 319
HinP1I GCGC 1 cut(s) 319
HincII GTYRAC 1 cut(s) 1927
HindII GTYRAC 1 cut(s) 1927
HpaII CCGG 3 cut(s) 372, 1839, 1977
HphI GGTGA 6 cut(s) 291, 332, 816, 1187, 1772, 2015
Hpy166II GTNNAC 5 cut(s) 225, 1368, 1800, 1919, 1927
Hpy188III TCNNGA 9 cut(s) 676, 692, 808, 886, 1118, 1301, 1523, 1570, 1592
Hpy8I GTNNAC 5 cut(s) 225, 1368, 1800, 1919, 1927
HpyAV CCTTC 5 cut(s) 317, 404, 623, 1674, 1893
HpyCH4III ACNGT 5 cut(s) 200, 1432, 1610, 1771, 1987
HpyF10VI GCNNNNNNNGC 6 cut(s) 28, 33, 1395, 1701, 1979, 1988
HpyF3I CTNAG 9 cut(s) 314, 448, 684, 704, 945, 1488, 1705, 2017, 2037
HspAI GCGC 1 cut(s) 319
Kzo9I GATC 4 cut(s) 453, 1447, 1640, 2010
LmnI GCTCC 7 cut(s) 481, 577, 985, 1047, 1594, 1742, 1979
Lsp1109I GCAGC 6 cut(s) 541, 569, 637, 1715, 1737, 1960
LweI GCATC 2 cut(s) 176, 1467
MabI ACCWGGT 2 cut(s) 1823, 1879
MaeI CTAG 1 cut(s) 380
MaeIII GTNAC 4 cut(s) 367, 822, 1136, 1578
MalI GATC 4 cut(s) 455, 1449, 1642, 2012
MboI GATC 4 cut(s) 453, 1447, 1640, 2010
MflI RGATCY 2 cut(s) 453, 2010
MhlI GDGCHC 4 cut(s) 387, 486, 1747, 1802
MlsI TGGCCA 1 cut(s) 750
MluNI TGGCCA 1 cut(s) 750
MlyI GAGTC 3 cut(s) 540, 543, 830
MmeI TCCRAC 3 cut(s) 185, 459, 1603
Mox20I TGGCCA 1 cut(s) 750
Mph1103I ATGCAT 3 cut(s) 191, 645, 1970
MscI TGGCCA 1 cut(s) 750
MseI TTAA 4 cut(s) 150, 603, 1734, 1949
MslI CAYNNNNRTG 2 cut(s) 644, 1134
Msp20I TGGCCA 1 cut(s) 750
MspA1I CMGCKG 2 cut(s) 560, 1728
MspI CCGG 3 cut(s) 372, 1839, 1977
MspR9I CCNGG 7 cut(s) 303, 360, 522, 576, 1296, 1825, 1881
Mva1269I GAATGC 1 cut(s) 1162
MvaI CCWGG 7 cut(s) 303, 360, 522, 576, 1296, 1825, 1881
MvnI CGCG 1 cut(s) 321
MwoI GCNNNNNNNGC 6 cut(s) 28, 33, 1395, 1701, 1979, 1988
NdeI CATATG 1 cut(s) 645
NdeII GATC 4 cut(s) 453, 1447, 1640, 2010
NlaIV GGNNCC 9 cut(s) 217, 291, 357, 516, 695, 771, 981, 1467, 1981
NmuCI GTSAC 2 cut(s) 822, 1136
NsiI ATGCAT 3 cut(s) 191, 645, 1970
NspI RCATGY 4 cut(s) 643, 1031, 1798, 1925
NspV TTCGAA 1 cut(s) 120
PaeR7I CTCGAG 1 cut(s) 1568
PagI TCATGA 3 cut(s) 807, 885, 1300
PceI AGGCCT 2 cut(s) 400, 466
PciI ACATGT 3 cut(s) 1027, 1794, 1921
PctI GAATGC 1 cut(s) 1162
PfeI GAWTC 7 cut(s) 296, 688, 995, 1055, 1265, 1328, 1626
PflMI CCANNNNNTGG 2 cut(s) 1082, 1809
PfoI TCCNGGA 1 cut(s) 520
PinAI ACCGGT 1 cut(s) 371
PkrI GCNGC 7 cut(s) 7, 556, 559, 652, 1727, 1730, 1975
PleI GAGTC 3 cut(s) 539, 543, 829
PpsI GAGTC 3 cut(s) 539, 543, 829
PscI ACATGT 3 cut(s) 1027, 1794, 1921
PshBI ATTAAT 1 cut(s) 1949
Psp124BI GAGCTC 1 cut(s) 387
Psp6I CCWGG 7 cut(s) 301, 358, 520, 574, 1294, 1823, 1879
PspFI CCCAGC 1 cut(s) 1050
PspGI CCWGG 7 cut(s) 301, 358, 520, 574, 1294, 1823, 1879
PspN4I GGNNCC 9 cut(s) 217, 291, 357, 516, 695, 771, 981, 1467, 1981
PspPI GGNCC 7 cut(s) 216, 355, 514, 694, 851, 896, 1557
PstI CTGCAG 1 cut(s) 559
PsuI RGATCY 2 cut(s) 453, 2010
PvuII CAGCTG 2 cut(s) 560, 1728
RsaI GTAC 5 cut(s) 226, 254, 1553, 1865, 1906
RsaNI GTAC 5 cut(s) 225, 253, 1552, 1864, 1905
RseI CAYNNNNRTG 2 cut(s) 644, 1134
SacI GAGCTC 1 cut(s) 387
SaqAI TTAA 4 cut(s) 150, 603, 1734, 1949
SatI GCNGC 7 cut(s) 6, 555, 558, 651, 1726, 1729, 1974
Sau3AI GATC 4 cut(s) 453, 1447, 1640, 2010
Sau96I GGNCC 7 cut(s) 216, 355, 514, 694, 851, 896, 1557
ScaI AGTACT 1 cut(s) 1553
SchI GAGTC 3 cut(s) 540, 543, 830
ScrFI CCNGG 7 cut(s) 303, 360, 522, 576, 1296, 1825, 1881
SduI GDGCHC 4 cut(s) 387, 486, 1747, 1802
SexAI ACCWGGT 2 cut(s) 1823, 1879
SfaNI GCATC 2 cut(s) 176, 1467
SfcI CTRYAG 1 cut(s) 555
Sfr274I CTCGAG 1 cut(s) 1568
SfuI TTCGAA 1 cut(s) 120
SinI GGWCC 7 cut(s) 216, 355, 514, 694, 851, 896, 1557
SlaI CTCGAG 1 cut(s) 1568
SmiMI CAYNNNNRTG 2 cut(s) 644, 1134
SmlI CTYRAG 1 cut(s) 1568
SmoI CTYRAG 1 cut(s) 1568
SseBI AGGCCT 2 cut(s) 400, 466
SsiI CCGC 2 cut(s) 1036, 1693
SspMI CTAG 1 cut(s) 380
SstI GAGCTC 1 cut(s) 387
StuI AGGCCT 2 cut(s) 400, 466
StyD4I CCNGG 7 cut(s) 301, 358, 520, 574, 1294, 1823, 1879
StyI CCWWGG 1 cut(s) 90
TaaI ACNGT 5 cut(s) 200, 1432, 1610, 1771, 1987
TaqI TCGA 5 cut(s) 120, 529, 819, 1569, 1953
TaqII GACCGA 1 cut(s) 884
TatI WGTACW 3 cut(s) 224, 252, 1551
TfiI GAWTC 7 cut(s) 296, 688, 995, 1055, 1265, 1328, 1626
Tru1I TTAA 4 cut(s) 150, 603, 1734, 1949
Tru9I TTAA 4 cut(s) 150, 603, 1734, 1949
TscAI CASTG 4 cut(s) 498, 688, 1174, 1990
TseFI GTSAC 2 cut(s) 822, 1136
TseI GCWGC 7 cut(s) 5, 554, 557, 650, 1725, 1728, 1973
Tsp45I GTSAC 2 cut(s) 822, 1136
TspDTI ATGAA 7 cut(s) 141, 516, 642, 796, 824, 902, 1247
TspGWI ACGGA 1 cut(s) 1922
TspRI CASTG 4 cut(s) 498, 688, 1174, 1990
Van91I CCANNNNNTGG 2 cut(s) 1082, 1809
VneI GTGCAC 1 cut(s) 1798
VpaK11BI GGWCC 7 cut(s) 216, 355, 514, 694, 851, 896, 1557
VspI ATTAAT 1 cut(s) 1949
XagI CCTNNNNNAGG 2 cut(s) 837, 1855
XapI RAATTY 3 cut(s) 525, 607, 1002
XceI RCATGY 4 cut(s) 643, 1031, 1798, 1925
XhoI CTCGAG 1 cut(s) 1568
XspI CTAG 1 cut(s) 380
ZrmI AGTACT 1 cut(s) 1553
Zsp2I ATGCAT 3 cut(s) 191, 645, 1970
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.