RchiOBHm_Chr5g0055861

Phospholipid diacylglycerol acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0011775
Physical Location & Seq
Forward (+)
59205920 .. 59209253
3334 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33280

Sequence Viewer

Length: 1086 bp
ATGGCTCCTGTTGTTTTAGATTCTGAAATTCTTGGGCTTCAAACCTTTGAACATGTCATGCGGGTATCTCGGAGCTGGGATTCCTTGATTTCCTTGTTGCCAAAAGGTGGAGAAACCATTTGGGGCAACTTGGAGTGGTCTCCTGAAGACGGGCATAGCTGTGACCTGATAAAGAAGAAATATCTGCATTCCACTGCTGGTGATACCAATTACAACAATAGCAATAAGAATATAATTTTCCAAGTAAAAGAACCTATGAAGTATGGAAGAATAATCTCATTTGGCAAGGAATCATCAGAATTACCCTCTTCAGAGCTTCCTGGTCATGATATAAAGGAACTTCTTTACAGGGATTCTCCTACCAATTTCAACTCATCATGTGGAGAGGTGTGGACTGAATATGATGAGGTAAGCAGGGAAAGCATAAGAAAAATTGCAGAAAATAAGGCTTACACAGTTCAAACCCTTTTTGATCTGCTACGCTTTGTGGCACCAAAAATGATGCAACGGGCTGAGGCTCACTTCTCTCATGGGATAGCTGATAATCTTGATAATCCTAAATATGACCATTACAAGTACTGGTCCAATCCACTCGAGACCAAGTTACCCATTGCTCCAGATTTGGAAATATACTGTTTATATGGTGTTGGACTCCCAACTGAAAGATCATACATATACAAGACATCTCCTTCTGACAAGTGCAAGAACATCCCATTCCGCATTGATAGCTCAGCAGATGGAGAGACAGGCAGCTGCCTTAAAAATGGAGCACATTTTGTTGATGGTGATGATACCGTGCCTGTATTGAGTGCTGGATACATGTGCACCAAAGGGTGGAGAGGAAGAACCAGGTTCAACCCATCCGGTATTGCTACCTACATAAGGGAGTACCAGCACAAGCGACCTGGTAGTTTTATTGAGGGAAGGGGTACGGAGAGTGGTTCACATGTTGACATCATGGGAAATGTGGCATTAATCGAAGATATCCTATGCATTGCTGCCGGAGCCACTGGTGCACAGATTGGAGGTGATAGGATCTACTCAGATATTATGAGAATGTCTGAGAGAATAAATCTAAAGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 map00561
Pfam Domains
Protein Families
LCAT Lipase_GDSL

Protein Analysis

361

Amino Acids

40.52

Weight (kDa)

5.85

Isoelectric Point (pI)

45.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCAT PF02450 20 - 321 3.2e-23 Lecithin:cholesterol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011775)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G44830
fragaria_vesca FvH4_3g30050
malus_domestica MD11G1154200.v1.1
prunus_persica Prupe.6G116700_v2.0.a1 Prupe.6G116700_v2.0.a1
pyrus_communis pycom11g12590
rosa_chinensis RchiOBHm_Chr5g0055851 RchiOBHm_Chr5g0055861
rosa_laevigata RLG00000035014
rosa_multiflora Rmu_sc0003306.1_g000008
rosa_roxburghii Rroxscaffold_1G00024510
rosa_rugosa Rorug05G0295200 Rorug05G0295200 Rorug05G0295300
rosa_samantha Rh5AG366600 Rh5BG376800 Rh5CG399800 Rh5DG389800
rosa_wichuraiana Rw5G034360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 490
AccB7I CCANNNNNTGG 2 cut(s) 107, 834
AciI CCGC 2 cut(s) 61, 718
AclWI GGATC 1 cut(s) 1043
AcsI RAATTY 1 cut(s) 27
AcuI CTGAAG 2 cut(s) 165, 294
AfaI GTAC 3 cut(s) 578, 890, 931
AfiI CCNNNNNNNGG 4 cut(s) 107, 149, 834, 882
AflIII ACRYGT 3 cut(s) 52, 819, 946
AgsI TTSAA 5 cut(s) 41, 50, 370, 461, 856
AjnI CCWGG 3 cut(s) 319, 848, 904
AluBI AGCT 7 cut(s) 75, 159, 316, 539, 729, 753, 1081
AluI AGCT 7 cut(s) 75, 159, 316, 539, 729, 753, 1081
Alw21I GWGCWC 3 cut(s) 772, 827, 1018
Alw26I GTCTC 3 cut(s) 144, 590, 737
Alw44I GTGCAC 2 cut(s) 823, 1014
AlwI GGATC 1 cut(s) 1043
Ama87I CYCGRG 1 cut(s) 593
ApaLI GTGCAC 2 cut(s) 823, 1014
ApeKI GCWGC 3 cut(s) 750, 753, 998
ApoI RAATTY 1 cut(s) 27
AseI ATTAAT 1 cut(s) 974
AspS9I GGNCC 1 cut(s) 582
AsuHPI GGTGA 3 cut(s) 212, 797, 1040
AvaI CYCGRG 1 cut(s) 593
AvaII GGWCC 1 cut(s) 582
BaeGI GKGCMC 2 cut(s) 827, 1018
BanI GGYRCC 1 cut(s) 490
BbsI GAAGAC 1 cut(s) 153
Bbv12I GWGCWC 3 cut(s) 772, 827, 1018
BbvCI CCTCAGC 1 cut(s) 513
BbvI GCAGC 3 cut(s) 740, 762, 985
BccI CCATC 3 cut(s) 731, 776, 868
BciT130I CCWGG 3 cut(s) 321, 850, 906
BciVI GTATCC 1 cut(s) 809
BcoDI GTCTC 3 cut(s) 144, 590, 737
BfuI GTATCC 1 cut(s) 809
BisI GCNGC 3 cut(s) 751, 754, 999
BlpI GCTNAGC 1 cut(s) 730
BlsI GCNGC 3 cut(s) 752, 755, 1000
BmcAI AGTACT 1 cut(s) 578
Bme1390I CCNGG 3 cut(s) 321, 850, 906
Bme18I GGWCC 1 cut(s) 582
BmeT110I CYCGRG 1 cut(s) 593
BmgT120I GGNCC 1 cut(s) 582
BmiI GGNNCC 3 cut(s) 6, 492, 1006
BmrFI CCNGG 3 cut(s) 321, 850, 906
BmsI GCATC 1 cut(s) 492
BpiI GAAGAC 1 cut(s) 153
BpmI CTGGAG 1 cut(s) 600
Bpu10I CCTNAGC 1 cut(s) 513
Bpu1102I GCTNAGC 1 cut(s) 730
BsaI GGTCTC 2 cut(s) 144, 590
BsaWI WCCGGW 1 cut(s) 863
Bsc4I CCNNNNNNNGG 4 cut(s) 107, 149, 834, 882
Bse1I ACTGG 2 cut(s) 584, 1015
Bse3DI GCAATG 2 cut(s) 609, 993
BseBI CCWGG 3 cut(s) 321, 850, 906
BseGI GGATG 2 cut(s) 708, 860
BseLI CCNNNNNNNGG 4 cut(s) 107, 149, 834, 882
BseMI GCAATG 2 cut(s) 609, 993
BseMII CTCAG 4 cut(s) 504, 744, 1053, 1056
BseNI ACTGG 2 cut(s) 584, 1015
BseSI GKGCMC 2 cut(s) 827, 1018
BseXI GCAGC 3 cut(s) 740, 762, 985
BseYI CCCAGC 1 cut(s) 75
BshNI GGYRCC 1 cut(s) 490
BsiHKAI GWGCWC 3 cut(s) 772, 827, 1018
BsiHKCI CYCGRG 1 cut(s) 593
BsiSI CCGG 2 cut(s) 864, 1002
BslI CCNNNNNNNGG 4 cut(s) 107, 149, 834, 882
BsmAI GTCTC 3 cut(s) 144, 590, 737
BsmI GAATGC 1 cut(s) 187
Bso31I GGTCTC 2 cut(s) 144, 590
BsoBI CYCGRG 1 cut(s) 593
Bsp1286I GDGCHC 3 cut(s) 772, 827, 1018
Bsp143I GATC 3 cut(s) 472, 665, 1035
Bsp1720I GCTNAGC 1 cut(s) 730
BspACI CCGC 2 cut(s) 61, 718
BspCNI CTCAG 4 cut(s) 505, 743, 1054, 1055
BspHI TCATGA 1 cut(s) 325
BspLI GGNNCC 3 cut(s) 6, 492, 1006
BspPI GGATC 1 cut(s) 1043
BspT107I GGYRCC 1 cut(s) 490
BspTNI GGTCTC 2 cut(s) 144, 590
BsrDI GCAATG 2 cut(s) 609, 993
BsrI ACTGG 2 cut(s) 584, 1015
BssMI GATC 3 cut(s) 472, 665, 1035
Bst2UI CCWGG 3 cut(s) 321, 850, 906
Bst4CI ACNGT 3 cut(s) 457, 635, 796
Bst6I CTCTTC 1 cut(s) 313
BstDEI CTNAG 4 cut(s) 513, 730, 1042, 1062
BstENI CCTNNNNNAGG 1 cut(s) 880
BstF5I GGATG 2 cut(s) 708, 860
BstKTI GATC 3 cut(s) 475, 668, 1038
BstMAI GTCTC 3 cut(s) 144, 590, 737
BstMBI GATC 3 cut(s) 472, 665, 1035
BstMWI GCNNNNNNNGC 4 cut(s) 420, 726, 1004, 1013
BstNI CCWGG 3 cut(s) 321, 850, 906
BstNSI RCATGY 3 cut(s) 56, 823, 950
BstSCI CCNGG 3 cut(s) 319, 848, 904
BstSLI GKGCMC 2 cut(s) 827, 1018
BstV1I GCAGC 3 cut(s) 740, 762, 985
BstV2I GAAGAC 1 cut(s) 153
BstX2I RGATCY 1 cut(s) 1035
BstYI RGATCY 1 cut(s) 1035
BsuI GTATCC 1 cut(s) 809
BtsCI GGATG 2 cut(s) 708, 860
BtsI GCAGTG 1 cut(s) 192
BtsIMutI CAGTG 2 cut(s) 192, 1008
CciI TCATGA 1 cut(s) 325
Cfr13I GGNCC 1 cut(s) 582
CsiI ACCWGGT 2 cut(s) 848, 904
Csp6I GTAC 3 cut(s) 577, 889, 930
CviAII CATG 8 cut(s) 53, 58, 326, 378, 530, 820, 947, 958
CviQI GTAC 3 cut(s) 577, 889, 930
DdeI CTNAG 4 cut(s) 513, 730, 1042, 1062
DpnI GATC 3 cut(s) 474, 667, 1037
DpnII GATC 3 cut(s) 472, 665, 1035
Eam1104I CTCTTC 1 cut(s) 313
EarI CTCTTC 1 cut(s) 313
Eco31I GGTCTC 2 cut(s) 144, 590
Eco32I GATATC 1 cut(s) 985
Eco47I GGWCC 1 cut(s) 582
Eco57I CTGAAG 2 cut(s) 165, 294
Eco88I CYCGRG 1 cut(s) 593
EcoNI CCTNNNNNAGG 1 cut(s) 880
EcoRII CCWGG 3 cut(s) 319, 848, 904
EcoRV GATATC 1 cut(s) 985
EcoT22I ATGCAT 1 cut(s) 995
FaeI CATG 8 cut(s) 56, 61, 329, 381, 533, 823, 950, 961
FatI CATG 8 cut(s) 52, 57, 325, 377, 529, 819, 946, 957
FauI CCCGC 1 cut(s) 54
Fnu4HI GCNGC 3 cut(s) 751, 754, 999
FokI GGATG 2 cut(s) 695, 847
Fsp4HI GCNGC 3 cut(s) 751, 754, 999
GluI GCNGC 3 cut(s) 751, 754, 999
GsaI CCCAGC 1 cut(s) 79
GsuI CTGGAG 1 cut(s) 600
HapII CCGG 2 cut(s) 864, 1002
Hin1II CATG 8 cut(s) 56, 61, 329, 381, 533, 823, 950, 961
HincII GTYRAC 1 cut(s) 952
HindII GTYRAC 1 cut(s) 952
HinfI GANTC 5 cut(s) 20, 80, 290, 353, 651
HpaII CCGG 2 cut(s) 864, 1002
HphI GGTGA 3 cut(s) 212, 797, 1040
Hpy166II GTNNAC 5 cut(s) 393, 825, 944, 952, 1016
Hpy188I TCNGA 7 cut(s) 25, 72, 298, 313, 694, 1045, 1063
Hpy188III TCNNGA 5 cut(s) 143, 326, 548, 595, 617
Hpy8I GTNNAC 5 cut(s) 393, 825, 944, 952, 1016
HpyAV CCTTC 2 cut(s) 699, 918
HpyCH4III ACNGT 3 cut(s) 457, 635, 796
HpyCH4V TGCA 7 cut(s) 187, 437, 505, 702, 825, 993, 1016
HpyF10VI GCNNNNNNNGC 4 cut(s) 420, 726, 1004, 1013
HpyF3I CTNAG 4 cut(s) 513, 730, 1042, 1062
Hsp92II CATG 8 cut(s) 56, 61, 329, 381, 533, 823, 950, 961
Kzo9I GATC 3 cut(s) 472, 665, 1035
LmnI GCTCC 5 cut(s) 10, 72, 619, 767, 1004
Lsp1109I GCAGC 3 cut(s) 740, 762, 985
LweI GCATC 1 cut(s) 492
MabI ACCWGGT 2 cut(s) 848, 904
MaeIII GTNAC 2 cut(s) 161, 603
MalI GATC 3 cut(s) 474, 667, 1037
MboI GATC 3 cut(s) 472, 665, 1035
MboII GAAGA 6 cut(s) 158, 187, 279, 300, 855, 992
MflI RGATCY 1 cut(s) 1035
MhlI GDGCHC 3 cut(s) 772, 827, 1018
MluCI AATT 6 cut(s) 27, 208, 234, 299, 364, 432
MlyI GAGTC 1 cut(s) 645
MmeI TCCRAC 1 cut(s) 628
MnlI CCTC 7 cut(s) 316, 379, 400, 508, 833, 913, 1019
Mph1103I ATGCAT 1 cut(s) 995
MseI TTAA 2 cut(s) 759, 974
MslI CAYNNNNRTG 1 cut(s) 159
MspA1I CMGCKG 1 cut(s) 753
MspI CCGG 2 cut(s) 864, 1002
MspR9I CCNGG 3 cut(s) 321, 850, 906
Mva1269I GAATGC 1 cut(s) 187
MvaI CCWGG 3 cut(s) 321, 850, 906
MwoI GCNNNNNNNGC 4 cut(s) 420, 726, 1004, 1013
NdeII GATC 3 cut(s) 472, 665, 1035
NlaIII CATG 8 cut(s) 56, 61, 329, 381, 533, 823, 950, 961
NlaIV GGNNCC 3 cut(s) 6, 492, 1006
NmuCI GTSAC 1 cut(s) 161
NsiI ATGCAT 1 cut(s) 995
NspI RCATGY 3 cut(s) 56, 823, 950
PaeR7I CTCGAG 1 cut(s) 593
PagI TCATGA 1 cut(s) 325
PciI ACATGT 3 cut(s) 52, 819, 946
PctI GAATGC 1 cut(s) 187
PfeI GAWTC 4 cut(s) 20, 80, 290, 353
PflMI CCANNNNNTGG 2 cut(s) 107, 834
PkrI GCNGC 3 cut(s) 752, 755, 1000
PleI GAGTC 1 cut(s) 645
PpsI GAGTC 1 cut(s) 645
PscI ACATGT 3 cut(s) 52, 819, 946
PshBI ATTAAT 1 cut(s) 974
Psp6I CCWGG 3 cut(s) 319, 848, 904
PspFI CCCAGC 1 cut(s) 75
PspGI CCWGG 3 cut(s) 319, 848, 904
PspN4I GGNNCC 3 cut(s) 6, 492, 1006
PspPI GGNCC 1 cut(s) 582
PsuI RGATCY 1 cut(s) 1035
PvuII CAGCTG 1 cut(s) 753
RsaI GTAC 3 cut(s) 578, 890, 931
RsaNI GTAC 3 cut(s) 577, 889, 930
RseI CAYNNNNRTG 1 cut(s) 159
SaqAI TTAA 2 cut(s) 759, 974
SatI GCNGC 3 cut(s) 751, 754, 999
Sau3AI GATC 3 cut(s) 472, 665, 1035
Sau96I GGNCC 1 cut(s) 582
ScaI AGTACT 1 cut(s) 578
SchI GAGTC 1 cut(s) 645
ScrFI CCNGG 3 cut(s) 321, 850, 906
SduI GDGCHC 3 cut(s) 772, 827, 1018
SexAI ACCWGGT 2 cut(s) 848, 904
SfaNI GCATC 1 cut(s) 492
Sfr274I CTCGAG 1 cut(s) 593
SinI GGWCC 1 cut(s) 582
SlaI CTCGAG 1 cut(s) 593
SmiMI CAYNNNNRTG 1 cut(s) 159
SmlI CTYRAG 1 cut(s) 593
SmoI CTYRAG 1 cut(s) 593
Sse9I AATT 6 cut(s) 27, 208, 234, 299, 364, 432
SsiI CCGC 2 cut(s) 61, 718
StyD4I CCNGG 3 cut(s) 319, 848, 904
TaaI ACNGT 3 cut(s) 457, 635, 796
TaqI TCGA 2 cut(s) 594, 978
TasI AATT 6 cut(s) 27, 208, 234, 299, 364, 432
TatI WGTACW 1 cut(s) 576
TfiI GAWTC 4 cut(s) 20, 80, 290, 353
Tru1I TTAA 2 cut(s) 759, 974
Tru9I TTAA 2 cut(s) 759, 974
TscAI CASTG 2 cut(s) 199, 1015
TseFI GTSAC 1 cut(s) 161
TseI GCWGC 3 cut(s) 750, 753, 998
Tsp45I GTSAC 1 cut(s) 161
TspDTI ATGAA 1 cut(s) 272
TspGWI ACGGA 1 cut(s) 947
TspRI CASTG 2 cut(s) 199, 1015
Van91I CCANNNNNTGG 2 cut(s) 107, 834
VneI GTGCAC 2 cut(s) 823, 1014
VpaK11BI GGWCC 1 cut(s) 582
VspI ATTAAT 1 cut(s) 974
XagI CCTNNNNNAGG 1 cut(s) 880
XapI RAATTY 1 cut(s) 27
XceI RCATGY 3 cut(s) 56, 823, 950
XhoI CTCGAG 1 cut(s) 593
ZrmI AGTACT 1 cut(s) 578
Zsp2I ATGCAT 1 cut(s) 995
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.