Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0006361

Physical Location & Seq

Genomic Coordinates Reverse (-)

19952723 .. 19968431

Gene Structure 15709 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1173100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2907 bp

ATGGGGCTTTTTCTTAAAGAAGCGCTCAAGCGGCTGTGCGGCGCCAATCAGTGGGCTTATGCTGTGTTCTGGAAGATCGGCTGCCAAAACCCTAAACTATTAATTTGGGAAGAATGTCATTACGAACCATCAATATCTTCTCTACCTAAACACATTGCTGGAACTGAAAGAGCTGAATTACCATTTGGAGAGTGGGAAGGATGCTGGGTTTCTTCTGAAGTGTGCTCCTCTTCTAATGGCATTCAACAAGAGGAAAGAGTGTCCTCACTTATTAACACAATGATGATTGATAAACCGTTTAATATAGTGGGCGAAGGAATAGTTGGACGAGCTGCATTTACGGGAAACCATCAATGGATTCTATCAGGCAATTACACAAAAGATGCCCATCCACCAGAGGTCCTGAATGAGATGCACCACCAATTTTCTGCTGGCATGCAGACAGTTGCAGTTATTCCTGTTCTTCCTCATGGTGTTGTTCAACTTGGTTCCTCCTCCGCTATGATGGAGAATATAGGATTCATCAATGATGTGAAAAGTTTAGTCCTGCAACTAGGACGCATTCCTGGTGCTCTTTTAGCAGAAAACTATGCGACAAAAGATTTGGTTGAGAAATCTGGGGTACCCAATGCTTCTGGAATAGTGGCATCAATGCATCCAGCAGGTAATTATAAGGTAACAGGATCCACACGGATGACTGATGACTACACCCATCAAAGCCACTTTACTCGGGCTTCAAGCCTATCTGGCCAACCATCTCACTCTCTTCTTAAAGATATTCAGAATAATTCTCAAACCACTTCTTCAACATTTCAAACCCCTAATCTTACCCAAAATCAGCCTAAAATTCATGATGATCCTCAACAACCAATGGTTTCTCCATTGATGAAGCTGAATTTTTCTTTGGGTGGCCAACTAAAGGATAGAGTTTGGGGAGCTGAAGTGATCACGTCAAATTCTGATGTGCGGTTGAACCAGTCAACTCCCTCTTTCAATTCTGGGGCGGGACTCAAATACCCGTCTGGTTTAGGTCAATCAGGTGCAGATCAGGACAGCCTAAAGTTTATAGAACATATGATCTTGTCTGGTGGTAGCATTAGGCACCATCTTGATAAAAACTTTAGCGCACCAAATGATATCATGCCTCAATTGGGAACAAATGGAAGTTTAATCTTTGATCAGAGCAAAGGTTCGAGAACAACCATGCTTGGAGGAAATCAAGCACATGGTGGATCGAGTTGTCATTCAAGGCCAATTTCTGTTCCTTGCTCTATTTCAGACCCCAACAGGGTAGCTGACATCAACCTCTGTGGTGGAAGTCTGTCCGGAGTTGAATTTCAAAAGGCTGATGCATTCCAAACAGGAGTTTCTTCATCCAGTGTAGCAGGTCAATTGGCTTCTAATAGCATGCCTTCCAAAGGCTCTGACCAGAGACAGTTTCCTGCTGATGTGACGCTTACACAGAATGAATTGGCCCCACAGAGGATAGATGATGAACTGTTTCATGCCCTTAATATCCCGTTGACACATCAAGATGAGCGTATCCCTTTGAATGCACACATCCCTGATATTGTTCTTGATGATTTTGATTATAAAATCTTCAGTCCAGGATCTGCAAATGCTACAAATGATGTGTGTACTCAGATTTCATCACGGGATGATTTGTTTGATGTTTTGGGTATGGATTTTAAAAATAAGCTGTTTAATGGCAATTGGAATAATTTGCTTGCTGGTGAGCCACACTCAAACACAAAAGACTTGGGTGAGAATACTTTGAAATGTACAAATGTGCAGGAGTTGGGTTCTGATTATTATTCAGCAGGTCAAGAAATCTCAAATGGTAGCATTTTCTCTGGGGCAGGTACTGACCATCTTTTAGATGCTGTGGTCTCTCGGGCTCAGCCTGCTGTCAAGCAGAACTCTGATGATAATGTGTCTTGTCGAACATCATTGACAAAAATCAGTAGCTCTACCATCCCCAATAGCTCTCCCACTTATGGACGGATAAGTATGTCTAATCATGTGCATGGGGAGAAATTTGGCCTTCATAAAACTCTTGTAAAAGCAGGCCTGGAAGAAACTAGTTCTTTCCAATCTGGCCGTAGCAGGGAGGATGCTGGAAACTGTTCTCAGACTAACTCCATATATGGATCTCAAATTAGTTCTTGGGAAGAACAGGGTAATAGGGCCAATCACGAGAGCAGTGTTTCAACAGCGTATTCTAAGAGGCCTGATACAGTAGGAAAATCAAATCGCAAAAGGCTTAAACCTGGAGAGAACCCTCGACCAAGGCCAAAAGATCGTCAGATGATCCAAGATCGTGTGAAGGAGTTGCGTGATATTGTGCCAAATGGTGCAAAATGTAGCATAGATGCACTGTTTGAACGCACCATCAAGCATATGCTTTTCTTGCAAAGCGTCACAAGGCATGCTGACAAGCTAAAACAAACGGGAGAGTCTAAGATTATTGGCACAGAAGATGGACTGGTTCTAAAAGACAACTTTGATGGAGGGGCGACATGGGCTTTTAAAGTTGGGACGCAATCAATGGTCTGCCCTATCATCGTTGAGGATCTCAATCCACCTCGTCAGATGCTTGTGGAGATGCTTTGTGAAGAACAGGGTTTCTTTTTAGAAATTGCTGATTTAATCAGAGGTTTGGGCTTGACCATCTTGAAGGGGGTAATGGAAGCTCGGAATGATAAGATATGGGCGCGCTTTGCAGTAGAGGCAAATAGGGACGTAACGAGGGTGGAAATATTTATGTCACTCGTTCACCTTTTGGAGCAAACTGTAAAAGGCACTGCGTTATTGGCCAATGCCATGAAGAACAATATGATGGTCCACCATTCGTTTGCTCAGGCAGCCCCAATACGTGCAACTGGCAGGCCTAGTAATTTGCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0008150 GO:0009653 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0010015 GO:0010073 GO:0010074 GO:0010078 GO:0010468 GO:0010479 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0019827 GO:0022622 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0080090 GO:0098727 GO:0099402 GO:0140110 GO:1902680 GO:1903506 GO:1903508 GO:1905392 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

bHLH-MYC_N

Protein Analysis

969

Amino Acids

105.6

Weight (kDa)

6.24

Isoelectric Point (pI)

35.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
bHLH-MYC_N	PF14215	5 - 181	1.1e-33	bHLH-MYC and R2R3-MYB transcription factors N-terminal
bHLH_LHW	PF23176	762 - 822	2.3e-33	Transcription factor LHW bHLH domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006361)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G27230 AT2G27230
fragaria_vesca	FvH4_3g26430 FvH4_3g26430 FvH4_3g26430 FvH4_3g26430 FvH4_3g26430 FvH4_3g26430 FvH4_3g26430 FvH4_3g26430
malus_domestica	MD03G1116900.v1.1 MD03G1155700.v1.1 MD11G1173100.v1.1
prunus_persica	Prupe.6G144300_v2.0.a1
pyrus_communis	pycom03g10980 pycom11g14740
rosa_chinensis	RchiOBHm_Chr5g0048491
rosa_laevigata	RLG00000034527
rosa_multiflora	Rmu_sc0001173.1_g000023
rosa_roxburghii	Rroxscaffold_1G00033350
rosa_rugosa	Rorug05G0238400 Rorug05G0238500
rosa_samantha	Rh5AG318800 Rh5BG328800 Rh5CG354800 Rh5DG339800
rosa_wichuraiana	Rw5G029780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	672, 1593
Acc36I	ACCTGC	4 cut(s)	653, 1376, 1811, 1850
Acc65I	GGTACC	1 cut(s)	622
AccB1I	GGYRCC	3 cut(s)	41, 622, 1101
AccB7I	CCANNNNNTGG	1 cut(s)	51
AccII	CGCG	1 cut(s)	2716
AccIII	TCCGGA	1 cut(s)	1325
AciI	CCGC	5 cut(s)	31, 39, 498, 967, 1004
AclWI	GGATC	8 cut(s)	678, 691, 851, 1240, 1618, 2158, 2305, 2580
AcoI	YGGCCR	4 cut(s)	748, 910, 2098, 2814
AcsI	RAATTY	5 cut(s)	846, 895, 955, 1334, 2036
AcuI	CTGAAG	3 cut(s)	237, 960, 1585
AcyI	GRCGYC	1 cut(s)	42
AdeI	CACNNNGTG	1 cut(s)	1229
AfaI	GTAC	4 cut(s)	624, 1639, 1783, 1864
AfeI	AGCGCT	1 cut(s)	24
AfiI	CCNNNNNNNGG	8 cut(s)	51, 919, 1151, 1288, 1418, 1482, 1997, 2107
AhlI	ACTAGT	1 cut(s)	2081
AjiI	CACGTC	1 cut(s)	951
AjnI	CCWGG	4 cut(s)	565, 1606, 2070, 2269
AjuI	GAANNNNNNNTTGG	6 cut(s)	168, 200, 306, 338, 887, 919
Alw21I	GWGCWC	2 cut(s)	227, 574
Alw26I	GTCTC	2 cut(s)	1426, 1894
AlwI	GGATC	8 cut(s)	678, 691, 851, 1240, 1618, 2158, 2305, 2580
AlwNI	CAGNNNCTG	2 cut(s)	1613, 1865
Ama87I	CYCGRG	2 cut(s)	729, 1893
Aor13HI	TCCGGA	1 cut(s)	1325
Aor51HI	AGCGCT	1 cut(s)	24
ApeKI	GCWGC	3 cut(s)	81, 332, 2864
ApoI	RAATTY	5 cut(s)	846, 895, 955, 1334, 2036
ArsI	GACNNNNNNTTYG	2 cut(s)	586, 618
AseI	ATTAAT	1 cut(s)	101
Asp700I	GAANNNNTTC	2 cut(s)	1500, 2125
Asp718I	GGTACC	1 cut(s)	622
AspLEI	GCGC	5 cut(s)	25, 44, 1127, 2716, 2718
AspS9I	GGNCC	4 cut(s)	400, 1474, 2187, 2842
AsuHPI	GGTGA	3 cut(s)	1745, 1775, 2768
AvaI	CYCGRG	2 cut(s)	729, 1893
AvaII	GGWCC	2 cut(s)	400, 2842
BalI	TGGCCA	3 cut(s)	750, 912, 2816
BamHI	GGATCC	1 cut(s)	683
BanI	GGYRCC	3 cut(s)	41, 622, 1101
BanII	GRGCYC	1 cut(s)	1900
BauI	CACGAG	1 cut(s)	2195
Bbv12I	GWGCWC	2 cut(s)	227, 574
BbvI	GCAGC	3 cut(s)	68, 319, 2876
BceAI	ACGGC	1 cut(s)	2085
BciT130I	CCWGG	4 cut(s)	567, 1608, 2072, 2271
BciVI	GTATCC	1 cut(s)	1553
BclI	TGATCA	2 cut(s)	945, 1177
BcoDI	GTCTC	2 cut(s)	1426, 1894
BcuI	ACTAGT	1 cut(s)	2081
BfaI	CTAG	3 cut(s)	554, 2082, 2892
BfoI	RGCGCY	2 cut(s)	26, 45
BfuAI	ACCTGC	4 cut(s)	653, 1376, 1811, 1850
BfuI	GTATCC	1 cut(s)	1553
BglI	GCCNNNNNGGC	1 cut(s)	747
BisI	GCNGC	5 cut(s)	32, 40, 82, 333, 2865
BlpI	GCTNAGC	1 cut(s)	1899
BlsI	GCNGC	5 cut(s)	33, 41, 83, 334, 2866
Bme1390I	CCNGG	4 cut(s)	567, 1608, 2072, 2271
Bme18I	GGWCC	2 cut(s)	400, 2842
BmeT110I	CYCGRG	2 cut(s)	729, 1893
BmgBI	CACGTC	1 cut(s)	951
BmgT120I	GGNCC	4 cut(s)	400, 1474, 2187, 2842
BmiI	GGNNCC	6 cut(s)	43, 490, 624, 685, 1103, 1476
BmrFI	CCNGG	4 cut(s)	567, 1608, 2072, 2271
BplI	GAGNNNNNCTC	2 cut(s)	1727, 1759
BpmI	CTGGAG	1 cut(s)	2292
Bpu10I	CCTNAGC	1 cut(s)	2859
Bpu1102I	GCTNAGC	1 cut(s)	1899
BpuEI	CTTGAG	1 cut(s)	11
BsaAI	YACGTR	1 cut(s)	2876
BsaBI	GATNNNNATC	2 cut(s)	387, 2577
BsaHI	GRCGYC	1 cut(s)	42
BsaI	GGTCTC	1 cut(s)	1894
BsaJI	CCNNGG	1 cut(s)	2288
BsaWI	WCCGGW	1 cut(s)	1325
BsaXI	ACNNNNNCTCC	2 cut(s)	927, 957
Bsc4I	CCNNNNNNNGG	8 cut(s)	51, 919, 1151, 1288, 1418, 1482, 1997, 2107
Bse1I	ACTGG	4 cut(s)	976, 1377, 2490, 2887
Bse3DI	GCAATG	1 cut(s)	153
Bse8I	GATNNNNATC	2 cut(s)	387, 2577
BseAI	TCCGGA	1 cut(s)	1325
BseBI	CCWGG	4 cut(s)	567, 1608, 2072, 2271
BseDI	CCNNGG	1 cut(s)	2288
BseGI	GGATG	9 cut(s)	206, 388, 655, 699, 1373, 1560, 1663, 1974, 2119
BseJI	GATNNNNATC	2 cut(s)	387, 2577
BseLI	CCNNNNNNNGG	8 cut(s)	51, 919, 1151, 1288, 1418, 1482, 1997, 2107
BseMI	GCAATG	1 cut(s)	153
BseMII	CTCAG	4 cut(s)	1655, 1913, 2144, 2873
BseNI	ACTGG	4 cut(s)	976, 1377, 2490, 2887
BsePI	GCGCGC	1 cut(s)	2714
BseRI	GAGGAG	2 cut(s)	217, 484
BseXI	GCAGC	3 cut(s)	68, 319, 2876
BseYI	CCCAGC	1 cut(s)	204
BsgI	GTGCAG	2 cut(s)	1062, 1811
Bsh1236I	CGCG	1 cut(s)	2716
BshNI	GGYRCC	3 cut(s)	41, 622, 1101
BsiHKAI	GWGCWC	2 cut(s)	227, 574
BsiHKCI	CYCGRG	2 cut(s)	729, 1893
BsiSI	CCGG	1 cut(s)	1326
BslFI	GGGAC	3 cut(s)	1020, 2551, 2753
BslI	CCNNNNNNNGG	8 cut(s)	51, 919, 1151, 1288, 1418, 1482, 1997, 2107
BsmAI	GTCTC	2 cut(s)	1426, 1894
BsmFI	GGGAC	3 cut(s)	1020, 2551, 2753
BsmI	GAATGC	4 cut(s)	240, 561, 1352, 1558
Bso31I	GGTCTC	1 cut(s)	1894
BsoBI	CYCGRG	2 cut(s)	729, 1893
Bsp1286I	GDGCHC	3 cut(s)	227, 574, 1900
Bsp13I	TCCGGA	1 cut(s)	1325
Bsp1407I	TGTACA	1 cut(s)	1781
Bsp1720I	GCTNAGC	1 cut(s)	1899
BspACI	CCGC	5 cut(s)	31, 39, 498, 967, 1004
BspCNI	CTCAG	4 cut(s)	1654, 1912, 2143, 2872
BspEI	TCCGGA	1 cut(s)	1325
BspFNI	CGCG	1 cut(s)	2716
BspHI	TCATGA	1 cut(s)	850
BspLI	GGNNCC	6 cut(s)	43, 490, 624, 685, 1103, 1476
BspMI	ACCTGC	4 cut(s)	653, 1376, 1811, 1850
BspPI	GGATC	8 cut(s)	678, 691, 851, 1240, 1618, 2158, 2305, 2580
BspT107I	GGYRCC	3 cut(s)	41, 622, 1101
BspTNI	GGTCTC	1 cut(s)	1894
BsrDI	GCAATG	1 cut(s)	153
BsrGI	TGTACA	1 cut(s)	1781
BsrI	ACTGG	4 cut(s)	976, 1377, 2490, 2887
BssECI	CCNNGG	1 cut(s)	2288
BssHII	GCGCGC	1 cut(s)	2714
BssNI	GRCGYC	1 cut(s)	42
BssSI	CACGAG	1 cut(s)	2195
BssT1I	CCWWGG	1 cut(s)	2288
Bst2BI	CACGAG	1 cut(s)	2195
Bst2UI	CCWGG	4 cut(s)	567, 1608, 2072, 2271
Bst4CI	ACNGT	8 cut(s)	297, 445, 1437, 1500, 2126, 2239, 2379, 2794
Bst6I	CTCTTC	2 cut(s)	235, 771
BstACI	GRCGYC	1 cut(s)	42
BstAUI	TGTACA	1 cut(s)	1781
BstBAI	YACGTR	1 cut(s)	2876
BstC8I	GCNNGC	9 cut(s)	433, 437, 1409, 1728, 1905, 2068, 2430, 2716, 2888
BstDEI	CTNAG	6 cut(s)	1641, 1899, 2130, 2223, 2460, 2859
BstENI	CCTNNNNNAGG	1 cut(s)	1416
BstF5I	GGATG	9 cut(s)	206, 388, 655, 699, 1373, 1560, 1663, 1974, 2119
BstFNI	CGCG	1 cut(s)	2716
BstH2I	RGCGCY	2 cut(s)	26, 45
BstHHI	GCGC	5 cut(s)	25, 44, 1127, 2716, 2718
BstMAI	GTCTC	2 cut(s)	1426, 1894
BstNI	CCWGG	4 cut(s)	567, 1608, 2072, 2271
BstNSI	RCATGY	3 cut(s)	439, 1411, 2432
BstSCI	CCNGG	4 cut(s)	565, 1606, 2070, 2269
BstUI	CGCG	1 cut(s)	2716
BstV1I	GCAGC	3 cut(s)	68, 319, 2876
BstX2I	RGATCY	4 cut(s)	683, 1610, 2150, 2572
BstYI	RGATCY	4 cut(s)	683, 1610, 2150, 2572
BsuI	GTATCC	1 cut(s)	1553
BtrI	CACGTC	1 cut(s)	951
BtsCI	GGATG	9 cut(s)	206, 388, 655, 699, 1373, 1560, 1663, 1974, 2119
BtsI	GCAGTG	2 cut(s)	2209, 2802
BtsIMutI	CAGTG	5 cut(s)	56, 1384, 2209, 2375, 2802
BveI	ACCTGC	4 cut(s)	653, 1376, 1811, 1850
Cac8I	GCNNGC	9 cut(s)	433, 437, 1409, 1728, 1905, 2068, 2430, 2716, 2888
CaiI	CAGNNNCTG	2 cut(s)	1613, 1865
CciI	TCATGA	1 cut(s)	850
CfoI	GCGC	5 cut(s)	25, 44, 1127, 2716, 2718
Cfr13I	GGNCC	4 cut(s)	400, 1474, 2187, 2842
CseI	GACGC	4 cut(s)	567, 1462, 2407, 2548
Csp6I	GTAC	4 cut(s)	623, 1638, 1782, 1863
CspCI	CAANNNNNGTGG	2 cut(s)	1291, 1326
CviQI	GTAC	4 cut(s)	623, 1638, 1782, 1863
DdeI	CTNAG	6 cut(s)	1641, 1899, 2130, 2223, 2460, 2859
DinI	GGCGCC	1 cut(s)	43
DraI	TTTAAA	2 cut(s)	1690, 2530
DraIII	CACNNNGTG	1 cut(s)	1229
EaeI	YGGCCR	4 cut(s)	748, 910, 2098, 2814
Eam1104I	CTCTTC	2 cut(s)	235, 771
EarI	CTCTTC	2 cut(s)	235, 771
Eco130I	CCWWGG	1 cut(s)	2288
Eco147I	AGGCCT	3 cut(s)	2070, 2230, 2890
Eco24I	GRGCYC	1 cut(s)	1900
Eco31I	GGTCTC	1 cut(s)	1894
Eco32I	GATATC	1 cut(s)	1138
Eco47I	GGWCC	2 cut(s)	400, 2842
Eco47III	AGCGCT	1 cut(s)	24
Eco57I	CTGAAG	3 cut(s)	237, 960, 1585
Eco88I	CYCGRG	2 cut(s)	729, 1893
EcoNI	CCTNNNNNAGG	1 cut(s)	1416
EcoO109I	RGGNCCY	1 cut(s)	400
EcoRII	CCWGG	4 cut(s)	565, 1606, 2070, 2269
EcoRV	GATATC	1 cut(s)	1138
EcoT14I	CCWWGG	1 cut(s)	2288
EcoT22I	ATGCAT	2 cut(s)	657, 1354
EcoT38I	GRGCYC	1 cut(s)	1900
EgeI	GGCGCC	1 cut(s)	43
EheI	GGCGCC	1 cut(s)	43
ErhI	CCWWGG	1 cut(s)	2288
FaqI	GGGAC	3 cut(s)	1020, 2551, 2753
FauI	CCCGC	1 cut(s)	997
FauNDI	CATATG	2 cut(s)	1074, 2400
FbaI	TGATCA	2 cut(s)	945, 1177
Fnu4HI	GCNGC	5 cut(s)	32, 40, 82, 333, 2865
FokI	GGATG	9 cut(s)	213, 375, 642, 706, 1360, 1547, 1670, 1961, 2126
FriOI	GRGCYC	1 cut(s)	1900
Fsp4HI	GCNGC	5 cut(s)	32, 40, 82, 333, 2865
FspBI	CTAG	3 cut(s)	554, 2082, 2892
GlaI	GCGC	5 cut(s)	24, 43, 1126, 2715, 2717
GluI	GCNGC	5 cut(s)	32, 40, 82, 333, 2865
GsaI	CCCAGC	1 cut(s)	208
GsuI	CTGGAG	1 cut(s)	2292
HaeII	RGCGCY	2 cut(s)	26, 45
HapII	CCGG	1 cut(s)	1326
HgaI	GACGC	4 cut(s)	567, 1462, 2407, 2548
HhaI	GCGC	5 cut(s)	25, 44, 1127, 2716, 2718
Hin1I	GRCGYC	1 cut(s)	42
Hin6I	GCGC	5 cut(s)	23, 42, 1125, 2714, 2716
HinP1I	GCGC	5 cut(s)	23, 42, 1125, 2714, 2716
HincII	GTYRAC	2 cut(s)	981, 1524
HindII	GTYRAC	2 cut(s)	981, 1524
HinfI	GANTC	4 cut(s)	358, 519, 1008, 2456
HpaII	CCGG	1 cut(s)	1326
HphI	GGTGA	3 cut(s)	1745, 1775, 2768
Hpy166II	GTNNAC	5 cut(s)	981, 1524, 1638, 2776, 2845
Hpy8I	GTNNAC	5 cut(s)	981, 1524, 1638, 2776, 2845
HpyAV	CCTTC	6 cut(s)	191, 308, 1422, 2054, 2320, 2671
HpyCH4III	ACNGT	8 cut(s)	297, 445, 1437, 1500, 2126, 2239, 2379, 2794
HpyCH4IV	ACGT	3 cut(s)	950, 2742, 2875
HpyF3I	CTNAG	6 cut(s)	1641, 1899, 2130, 2223, 2460, 2859
HpySE526I	ACGT	3 cut(s)	950, 2742, 2875
Hsp92I	GRCGYC	1 cut(s)	42
HspAI	GCGC	5 cut(s)	23, 42, 1125, 2714, 2716
KasI	GGCGCC	1 cut(s)	41
Kpn2I	TCCGGA	1 cut(s)	1325
KpnI	GGTACC	1 cut(s)	626
Ksp22I	TGATCA	2 cut(s)	945, 1177
LmnI	GCTCC	3 cut(s)	230, 935, 2785
Lsp1109I	GCAGC	3 cut(s)	68, 319, 2876
MaeI	CTAG	3 cut(s)	554, 2082, 2892
MaeII	ACGT	3 cut(s)	950, 2742, 2875
MaeIII	GTNAC	5 cut(s)	676, 1450, 2419, 2743, 2766
MfeI	CAATTG	3 cut(s)	1148, 1391, 1711
MflI	RGATCY	4 cut(s)	683, 1610, 2150, 2572
MhlI	GDGCHC	3 cut(s)	227, 574, 1900
MlsI	TGGCCA	3 cut(s)	750, 912, 2816
MluNI	TGGCCA	3 cut(s)	750, 912, 2816
Mly113I	GGCGCC	1 cut(s)	42
MlyI	GAGTC	2 cut(s)	1002, 2465
MmeI	TCCRAC	1 cut(s)	304
Mox20I	TGGCCA	3 cut(s)	750, 912, 2816
Mph1103I	ATGCAT	2 cut(s)	657, 1354
MroI	TCCGGA	1 cut(s)	1325
MroXI	GAANNNNTTC	2 cut(s)	1500, 2125
MscI	TGGCCA	3 cut(s)	750, 912, 2816
MslI	CAYNNNNRTG	4 cut(s)	281, 692, 1533, 2025
Msp20I	TGGCCA	3 cut(s)	750, 912, 2816
MspI	CCGG	1 cut(s)	1326
MspR9I	CCNGG	4 cut(s)	567, 1608, 2072, 2271
MunI	CAATTG	3 cut(s)	1148, 1391, 1711
Mva1269I	GAATGC	4 cut(s)	240, 561, 1352, 1558
MvaI	CCWGG	4 cut(s)	567, 1608, 2072, 2271
MvnI	CGCG	1 cut(s)	2716
NarI	GGCGCC	1 cut(s)	42
NdeI	CATATG	2 cut(s)	1074, 2400
NlaIV	GGNNCC	6 cut(s)	43, 490, 624, 685, 1103, 1476
NmuCI	GTSAC	3 cut(s)	1450, 2419, 2766
NsiI	ATGCAT	2 cut(s)	657, 1354
NspI	RCATGY	3 cut(s)	439, 1411, 2432
PaeI	GCATGC	3 cut(s)	439, 1411, 2432
PagI	TCATGA	1 cut(s)	850
PauI	GCGCGC	1 cut(s)	2714
PceI	AGGCCT	3 cut(s)	2070, 2230, 2890
PctI	GAATGC	4 cut(s)	240, 561, 1352, 1558
PdmI	GAANNNNTTC	2 cut(s)	1500, 2125
PfeI	GAWTC	2 cut(s)	358, 519
PflMI	CCANNNNNTGG	1 cut(s)	51
PfoI	TCCNGGA	1 cut(s)	1606
PkrI	GCNGC	5 cut(s)	33, 41, 83, 334, 2866
PleI	GAGTC	2 cut(s)	1002, 2464
PluTI	GGCGCC	1 cut(s)	45
PpsI	GAGTC	2 cut(s)	1002, 2464
Ppu21I	YACGTR	1 cut(s)	2876
PpuMI	RGGWCCY	1 cut(s)	400
PshBI	ATTAAT	1 cut(s)	101
PsiI	TTATAA	2 cut(s)	672, 1593
Psp5II	RGGWCCY	1 cut(s)	400
Psp6I	CCWGG	4 cut(s)	565, 1606, 2070, 2269
PspFI	CCCAGC	1 cut(s)	204
PspGI	CCWGG	4 cut(s)	565, 1606, 2070, 2269
PspN4I	GGNNCC	6 cut(s)	43, 490, 624, 685, 1103, 1476
PspPI	GGNCC	4 cut(s)	400, 1474, 2187, 2842
PspPPI	RGGWCCY	1 cut(s)	400
PstNI	CAGNNNCTG	2 cut(s)	1613, 1865
PsuI	RGATCY	4 cut(s)	683, 1610, 2150, 2572
PteI	GCGCGC	1 cut(s)	2714
RsaI	GTAC	4 cut(s)	624, 1639, 1783, 1864
RsaNI	GTAC	4 cut(s)	623, 1638, 1782, 1863
RseI	CAYNNNNRTG	4 cut(s)	281, 692, 1533, 2025
SatI	GCNGC	5 cut(s)	32, 40, 82, 333, 2865
Sau96I	GGNCC	4 cut(s)	400, 1474, 2187, 2842
SchI	GAGTC	2 cut(s)	1002, 2465
ScrFI	CCNGG	4 cut(s)	567, 1608, 2072, 2271
SduI	GDGCHC	3 cut(s)	227, 574, 1900
SfoI	GGCGCC	1 cut(s)	43
SinI	GGWCC	2 cut(s)	400, 2842
SmiMI	CAYNNNNRTG	4 cut(s)	281, 692, 1533, 2025
SmlI	CTYRAG	1 cut(s)	26
SmoI	CTYRAG	1 cut(s)	26
SpeI	ACTAGT	1 cut(s)	2081
SphI	GCATGC	3 cut(s)	439, 1411, 2432
SseBI	AGGCCT	3 cut(s)	2070, 2230, 2890
SsiI	CCGC	5 cut(s)	31, 39, 498, 967, 1004
SspDI	GGCGCC	1 cut(s)	41
SspI	AATATT	1 cut(s)	2760
SspMI	CTAG	3 cut(s)	554, 2082, 2892
StuI	AGGCCT	3 cut(s)	2070, 2230, 2890
StyD4I	CCNGG	4 cut(s)	565, 1606, 2070, 2269
StyI	CCWWGG	1 cut(s)	2288
TaaI	ACNGT	8 cut(s)	297, 445, 1437, 1500, 2126, 2239, 2379, 2794
TaiI	ACGT	3 cut(s)	953, 2745, 2878
TaqI	TCGA	4 cut(s)	1193, 1235, 1942, 2284
TatI	WGTACW	2 cut(s)	1637, 1781
TauI	GCSGC	2 cut(s)	34, 42
TfiI	GAWTC	2 cut(s)	358, 519
TscAI	CASTG	5 cut(s)	56, 1384, 2209, 2382, 2809
TseFI	GTSAC	3 cut(s)	1450, 2419, 2766
TseI	GCWGC	3 cut(s)	81, 332, 2864
Tsp45I	GTSAC	3 cut(s)	1450, 2419, 2766
TspGWI	ACGGA	2 cut(s)	706, 2017
TspRI	CASTG	5 cut(s)	56, 1384, 2209, 2382, 2809
Van91I	CCANNNNNTGG	1 cut(s)	51
VpaK11BI	GGWCC	2 cut(s)	400, 2842
VspI	ATTAAT	1 cut(s)	101
XagI	CCTNNNNNAGG	1 cut(s)	1416
XapI	RAATTY	5 cut(s)	846, 895, 955, 1334, 2036
XceI	RCATGY	3 cut(s)	439, 1411, 2432
XcmI	CCANNNNNNNNNTGG	2 cut(s)	189, 428
XmnI	GAANNNNTTC	2 cut(s)	1500, 2125
XspI	CTAG	3 cut(s)	554, 2082, 2892
Zsp2I	ATGCAT	2 cut(s)	657, 1354

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.