Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0006361

Physical Location & Seq

Genomic Coordinates Reverse (-)

46428548 .. 46436683

Gene Structure 8136 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G029780.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2886 bp

ATGGGCGTTTTGCTCAAAGAAGCGCTTAAGAGGCTGTGCAGTGCCAATCAGTGGGCTTATGCCGTCTTCTGGAAGATCGGCTGCCAAAACCCTAATTTGTTAATTTGGGAAGAATCTCATTATGAACCATCACTATCGTCTCGACATACACGCATTGCTGGAACTGAAAGTGGTGATTCACCATTTGGTGAGTGGGAAGGATGCTGGGTTTCCTCTAAAGTGTGTTCATCTTCTCATGGCATTCAACCAGAGGAAAGAGTCTCTGCACTTCTTAACAAAATGTTGATGGATAAACCATTTACAATAGTGGGCGACGGGATCGTTGGACGAGCTGCATTTACTGGACATCATCAATGGATTCTTTCAAGCAATTACTCTAAATATGCCCATCCACCAGAGGTCCTAAATGAGATGCACCACCAATTTTCAGCTGGAATGCAGACAGTTGCAGTTATTCCTGTTCTCCCTCACGGCGTTGTTCAGCTTGGTTCTAGTTTGGCTATTATGGAGAGTATAGGATTCATTAATGACGTCAAGAGTTTGATCCTGCAACTTGGATGTGTTCCTGGTGCTCTTTTATCTGAAAATTATGAGACAAAAAGTTCAGATGAGAAAGTTGGGGTACCTTATACTATTGGAATGCTGACAACTGTTCATCCAGGCGGACACACATCAAGTTCTACTCATACGACGAGTAGCAGCAATCATCAAAAGCACCCATCTCAGGCTTCCAGGCTTGTTGGTCAACCTTCTCATTCTCTTTATGAAGAGATTCAGAATATTTCTCAAGCTACTGCTTCAACATTTCAAACTCCTAACCTTACCCAAATTCTGCCTAAAATTCATGATGACCGTCGACAACCAGTGATGAAGCCAAAGTTTCCTCTTGGAGGCCAACTAAGGGATGGAGTTGGGGTAGCTGAAGTGATCTCCTCCAATTCTGATGCATGGTTGAACCACCTTGCTCCTTCTAATTTGAGGTGTGGACTCAAGTACCCATGTGGTTCTGTTCAATCAGCTGCAAGTCAGGGAAGCTTAGAGTTGATGGAACATCAGATCTTGTCTGGTGGTAGCATGAGGCCTACTTTTAGTAGCAAAATTAGTGCATCAAACGGTTTCATGACTGAGCCTAGAACAGGTGGAATTCTAACCCTTGATCAGAGAAAAAGTTTGATAACCACTCCAGCATTGCTTGGGGGAAGTCAAACACATGGTGGATCGAGTACTCATTCGAGGCCAACTTCAGCTACTTGTTCTCATATAGAGACACACGGGACAGCTGACATTAATCTCTCTGGAACATATCTGTCTGGGGTTGAATTTCAAAAGGCTGATTCATTCAAAACAGACAGGATTTCTTTATCTAGTTTGGCTGATCAATCAGCTTCTCAGGATATACTTTCAAAAGGCTCTGACCAGAGGCAGTTTTCCACGGAAGTGAAGCTTAATCAGAGTGATTTGCCTCCAGTGGAGCAAAGGACGGATAATAAATTGTTTCAAGTCCCATTGACTCATCCATGTGAACATGTATCTTCGAGTGATCACATACCTCAGATTCATGATGGTCTTGATAATAAAATTTGTACCCCAGGATCTGTAAATGCTACAAACGATGATATGTGTACTCAATCAGGGGATGACTTGTTTGATGTTTTGGGTGTGGATTTCAAAAATAAGTTGCTTAATGGTAATTGGAATAGTTTGCTGGCTGATGAACCAGGTTCAAACAAAAGAGATCTTGCTGAGACTACTTTGGCATTCACAAATGTGAAGGATCTGGTTTCTGATTATTATTCAGCAAGTGAAGGCATATCAAACAGTAGTATCTTCTCTGGGGCAGATGCTGACCATCTTTTAGATGCTGTGGTCTCGAAGGCGCACTCTACTATCAAGCAGAGCTCTGATGATAATGTGTCTTGTAAAACAACATTGACAAAAATCAGTACCTCTTCTGTCCCTAGTGGTTCTCTCACTTATGGACAGGTTAGCGTGTCTAATCAAGTGCCGGGGGAGACATTTGACCTTCCTAAGGATCTTGGAAAAGCAGGCTTAGTAGAAATCAGTTCTTTCCTATCTGGGTGTAGCAGGAATGATGCAGGAAACTGTTCACAGACTACCTCCATATATGGATCTCAAGTTAGTTCTTGGGTTGAACAGGGCAATAATTCAAAGCATGATAGTAGTGTTTCAACTGCATATTCTAAGAAGCCTGATCTAATGGGGAAATCAAATCGCAAAAGGCTGAAACCTGGCGAGAATCCTAGACCAAGACCAAAAGATCGCCAGATGATCCAAGATCGTGTGAAGGAGTTGCGTGACATTGTGCCAAATGGAGCAAAATGTAGCATAGATGCACTGCTCGAACGTACCATCAAGCATATGCTTTTCTTGCAAAGTGTCACTAAGCATGCTGACAAGCTAAAACAAACAGGAAGAGCCAAGATTATTGGCAAAGAAGGTGGACTGCATCTAAAAGATAACTATGATGGAAGGGCAACATGGGCTTTTGATGTTGGGTCACAATCTATGGTCTGCCCCATCATAGTTGAGGATCTAGATTCACCTCGCCAGATGCTTGTGGAGATGCTTTGTGAAGAACAGGGTTTCTTTTTAGAAATTGCTGATATAATTAGAGGTTTGGGTTTGACCATCTTGAAAGGGGTGATGGAAACTCGGAATGATAAGATATGGGCACGCTTTGCTGTAGAGGCAAATAGGGATGTAACAAGGATGGAAATATTTATGTCCCTTGTTCACCTTTTGGAGCAAACTGTAAAAGGCACCACGACATCAGTCAATGCTATCAAGAACAACAGTATGGTCCACCATTCTTTCGCCCAAGCAGCCCCAATACCTGCAACTGGTAGGCCCAGTGGTTTTCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0008150 GO:0009653 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0010015 GO:0010073 GO:0010074 GO:0010078 GO:0010468 GO:0010479 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0019827 GO:0022622 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0080090 GO:0098727 GO:0099402 GO:0140110 GO:1902680 GO:1903506 GO:1903508 GO:1905392 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

bHLH-MYC_N

Protein Analysis

961

Amino Acids

104.3

Weight (kDa)

6.41

Isoelectric Point (pI)

37.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
bHLH-MYC_N	PF14215	5 - 181	6.6e-33	bHLH-MYC and R2R3-MYB transcription factors N-terminal
bHLH_LHW	PF23176	755 - 814	7.8e-34	Transcription factor LHW bHLH domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006361)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G27230 AT2G27230
fragaria_vesca	FvH4_3g26430 FvH4_3g26430 FvH4_3g26430 FvH4_3g26430 FvH4_3g26430 FvH4_3g26430 FvH4_3g26430 FvH4_3g26430
malus_domestica	MD03G1116900.v1.1 MD03G1155700.v1.1 MD11G1173100.v1.1
prunus_persica	Prupe.6G144300_v2.0.a1
pyrus_communis	pycom03g10980 pycom11g14740
rosa_chinensis	RchiOBHm_Chr5g0048491
rosa_laevigata	RLG00000034527
rosa_multiflora	Rmu_sc0001173.1_g000023
rosa_roxburghii	Rroxscaffold_1G00033350
rosa_rugosa	Rorug05G0238400 Rorug05G0238500
rosa_samantha	Rh5AG318800 Rh5BG328800 Rh5CG354800 Rh5DG339800
rosa_wichuraiana	Rw5G029780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	534
Acc36I	ACCTGC	1 cut(s)	2863
Acc65I	GGTACC	1 cut(s)	622
AccB1I	GGYRCC	2 cut(s)	622, 2780
AccB7I	CCANNNNNTGG	1 cut(s)	51
AccI	GTMKAC	1 cut(s)	856
AciI	CCGC	1 cut(s)	663
AclWI	GGATC	9 cut(s)	326, 538, 1225, 1600, 1782, 2040, 2137, 2284, 2559
AcsI	RAATTY	5 cut(s)	828, 840, 1143, 1319, 1578
AcuI	CTGAAG	2 cut(s)	942, 1227
AcyI	GRCGYC	1 cut(s)	531
AdeI	CACNNNGTG	1 cut(s)	1214
AfaI	GTAC	7 cut(s)	624, 995, 1225, 1585, 1624, 1945, 2368
AfeI	AGCGCT	1 cut(s)	24
AfiI	CCNNNNNNNGG	8 cut(s)	51, 69, 724, 890, 901, 1136, 2029, 2861
AflII	CTTAAG	1 cut(s)	26
AflIII	ACRYGT	1 cut(s)	1525
AjnI	CCWGG	6 cut(s)	565, 658, 731, 1588, 1717, 2248
AleI	CACNNNNGTG	2 cut(s)	1436, 1766
Alw21I	GWGCWC	2 cut(s)	574, 1901
Alw26I	GTCTC	7 cut(s)	144, 265, 587, 1259, 1739, 1873, 2006
AlwI	GGATC	9 cut(s)	326, 538, 1225, 1600, 1782, 2040, 2137, 2284, 2559
AlwNI	CAGNNNCTG	2 cut(s)	1595, 1844
Aor51HI	AGCGCT	1 cut(s)	24
AoxI	GGCC	4 cut(s)	892, 1079, 1235, 2867
ApeKI	GCWGC	5 cut(s)	81, 332, 699, 1019, 2843
ApoI	RAATTY	5 cut(s)	828, 840, 1143, 1319, 1578
AseI	ATTAAT	2 cut(s)	525, 1287
Asp700I	GAANNNNTTC	2 cut(s)	771, 2104
Asp718I	GGTACC	1 cut(s)	622
AspLEI	GCGC	2 cut(s)	25, 1879
AspS9I	GGNCC	3 cut(s)	400, 2821, 2868
AsuC2I	CCSGG	1 cut(s)	2007
AsuHPI	GGTGA	6 cut(s)	171, 185, 200, 2553, 2674, 2747
AvaII	GGWCC	2 cut(s)	400, 2821
AxyI	CCTNAGG	1 cut(s)	2028
BaeGI	GKGCMC	1 cut(s)	2695
BaeI	ACNNNNGTAYC	4 cut(s)	977, 1010, 1807, 1840
BanI	GGYRCC	2 cut(s)	622, 2780
BanII	GRGCYC	1 cut(s)	1901
BbsI	GAAGAC	1 cut(s)	58
Bbv12I	GWGCWC	2 cut(s)	574, 1901
BbvI	GCAGC	5 cut(s)	68, 319, 711, 1006, 2855
BceAI	ACGGC	2 cut(s)	47, 487
BciT130I	CCWGG	6 cut(s)	567, 660, 733, 1590, 1719, 2250
BclI	TGATCA	3 cut(s)	1156, 1375, 1540
BcnI	CCSGG	1 cut(s)	2007
BcoDI	GTCTC	7 cut(s)	144, 265, 587, 1259, 1739, 1873, 2006
BfaI	CTAG	6 cut(s)	492, 1131, 1365, 1959, 2262, 2555
BfmI	CTRYAG	1 cut(s)	2703
BfoI	RGCGCY	1 cut(s)	26
BfrI	CTTAAG	1 cut(s)	26
BfuAI	ACCTGC	1 cut(s)	2863
BglII	AGATCT	2 cut(s)	1056, 1735
BisI	GCNGC	5 cut(s)	82, 333, 700, 1020, 2844
BlsI	GCNGC	5 cut(s)	83, 334, 701, 1021, 2845
BmcAI	AGTACT	1 cut(s)	1225
Bme1390I	CCNGG	7 cut(s)	567, 660, 733, 1590, 1719, 2007, 2250
Bme18I	GGWCC	2 cut(s)	400, 2821
BmgT120I	GGNCC	3 cut(s)	400, 2821, 2868
BmiI	GGNNCC	2 cut(s)	624, 2782
BmrFI	CCNGG	7 cut(s)	567, 660, 733, 1590, 1719, 2007, 2250
BmrI	ACTGGG	1 cut(s)	2865
BmuI	ACTGGG	1 cut(s)	2865
BoxI	GACNNNNGTC	1 cut(s)	2792
BpiI	GAAGAC	1 cut(s)	58
BpmI	CTGGAG	2 cut(s)	1167, 1449
BpuEI	CTTGAG	3 cut(s)	771, 974, 2118
BpuMI	CCSGG	1 cut(s)	2007
BsaHI	GRCGYC	1 cut(s)	531
BsaI	GGTCTC	1 cut(s)	1873
BsaJI	CCNNGG	3 cut(s)	1431, 1588, 2006
BsaXI	ACNNNNNCTCC	2 cut(s)	900, 930
Bsc4I	CCNNNNNNNGG	8 cut(s)	51, 69, 724, 890, 901, 1136, 2029, 2861
Bse1I	ACTGG	5 cut(s)	346, 863, 1466, 2866, 2871
Bse21I	CCTNAGG	1 cut(s)	2028
Bse3DI	GCAATG	2 cut(s)	153, 1187
BseBI	CCWGG	6 cut(s)	567, 660, 733, 1590, 1719, 2250
BseDI	CCNNGG	3 cut(s)	1431, 1588, 2006
BseGI	GGATG	9 cut(s)	206, 388, 563, 655, 910, 1513, 1642, 2725, 2736
BseLI	CCNNNNNNNGG	8 cut(s)	51, 69, 724, 890, 901, 1136, 2029, 2861
BseMI	GCAATG	2 cut(s)	153, 1187
BseMII	CTCAG	5 cut(s)	737, 1116, 1403, 1565, 1734
BseNI	ACTGG	5 cut(s)	346, 863, 1466, 2866, 2871
BseRI	GAGGAG	1 cut(s)	922
BseSI	GKGCMC	1 cut(s)	2695
BseXI	GCAGC	5 cut(s)	68, 319, 711, 1006, 2855
BseYI	CCCAGC	1 cut(s)	204
BsgI	GTGCAG	2 cut(s)	58, 249
BshFI	GGCC	4 cut(s)	894, 1081, 1237, 2869
BshNI	GGYRCC	2 cut(s)	622, 2780
BsiHKAI	GWGCWC	2 cut(s)	574, 1901
BsiSI	CCGG	1 cut(s)	2006
BslFI	GGGAC	4 cut(s)	1288, 1487, 1940, 2731
BslI	CCNNNNNNNGG	8 cut(s)	51, 69, 724, 890, 901, 1136, 2029, 2861
BsmAI	GTCTC	7 cut(s)	144, 265, 587, 1259, 1739, 1873, 2006
BsmBI	CGTCTC	1 cut(s)	144
BsmFI	GGGAC	4 cut(s)	1288, 1487, 1940, 2731
BsmI	GAATGC	4 cut(s)	240, 441, 645, 1757
BsnI	GGCC	4 cut(s)	894, 1081, 1237, 2869
Bso31I	GGTCTC	1 cut(s)	1873
Bsp1286I	GDGCHC	3 cut(s)	574, 1901, 2695
BspACI	CCGC	1 cut(s)	663
BspANI	GGCC	4 cut(s)	894, 1081, 1237, 2869
BspCNI	CTCAG	5 cut(s)	736, 1117, 1402, 1564, 1735
BspHI	TCATGA	3 cut(s)	844, 1119, 1558
BspLI	GGNNCC	2 cut(s)	624, 2782
BspMI	ACCTGC	1 cut(s)	2863
BspPI	GGATC	9 cut(s)	326, 538, 1225, 1600, 1782, 2040, 2137, 2284, 2559
BspQI	GCTCTTC	1 cut(s)	2428
BspT107I	GGYRCC	2 cut(s)	622, 2780
BspTI	CTTAAG	1 cut(s)	26
BspTNI	GGTCTC	1 cut(s)	1873
BsrDI	GCAATG	2 cut(s)	153, 1187
BsrI	ACTGG	5 cut(s)	346, 863, 1466, 2866, 2871
BssECI	CCNNGG	3 cut(s)	1431, 1588, 2006
BssNI	GRCGYC	1 cut(s)	531
Bst2UI	CCWGG	6 cut(s)	567, 660, 733, 1590, 1719, 2250
Bst4CI	ACNGT	8 cut(s)	445, 652, 854, 1115, 1820, 2105, 2773, 2816
Bst6I	CTCTTC	3 cut(s)	762, 1954, 2428
BstACI	GRCGYC	1 cut(s)	531
BstAFI	CTTAAG	1 cut(s)	26
BstAPI	GCANNNNNTGC	1 cut(s)	2699
BstC8I	GCNNGC	4 cut(s)	1707, 2047, 2409, 2695
BstDSI	CCRYGG	1 cut(s)	1431
BstENI	CCTNNNNNAGG	3 cut(s)	888, 1134, 2027
BstF5I	GGATG	9 cut(s)	206, 388, 563, 655, 910, 1513, 1642, 2725, 2736
BstH2I	RGCGCY	1 cut(s)	26
BstHHI	GCGC	2 cut(s)	25, 1879
BstMAI	GTCTC	7 cut(s)	144, 265, 587, 1259, 1739, 1873, 2006
BstMWI	GCNNNNNNNGC	6 cut(s)	31, 2389, 2501, 2699, 2708, 2843
BstNI	CCWGG	6 cut(s)	567, 660, 733, 1590, 1719, 2250
BstNSI	RCATGY	2 cut(s)	1529, 2411
BstPAI	GACNNNNGTC	1 cut(s)	2792
BstSCI	CCNGG	7 cut(s)	565, 658, 731, 1588, 1717, 2005, 2248
BstSFI	CTRYAG	1 cut(s)	2703
BstSLI	GKGCMC	1 cut(s)	2695
BstV1I	GCAGC	5 cut(s)	68, 319, 711, 1006, 2855
BstV2I	GAAGAC	1 cut(s)	58
BstX2I	RGATCY	7 cut(s)	1056, 1592, 1735, 1774, 2032, 2129, 2551
BstYI	RGATCY	7 cut(s)	1056, 1592, 1735, 1774, 2032, 2129, 2551
Bsu36I	CCTNAGG	1 cut(s)	2028
BsuRI	GGCC	4 cut(s)	894, 1081, 1237, 2869
BtgI	CCRYGG	1 cut(s)	1431
BtsCI	GGATG	9 cut(s)	206, 388, 563, 655, 910, 1513, 1642, 2725, 2736
BtsI	GCAGTG	2 cut(s)	46, 2354
BtsIMutI	CAGTG	6 cut(s)	46, 56, 870, 1473, 2354, 2878
BveI	ACCTGC	1 cut(s)	2863
Cac8I	GCNNGC	4 cut(s)	1707, 2047, 2409, 2695
CaiI	CAGNNNCTG	2 cut(s)	1595, 1844
CciI	TCATGA	3 cut(s)	844, 1119, 1558
CfoI	GCGC	2 cut(s)	25, 1879
Cfr13I	GGNCC	3 cut(s)	400, 2821, 2868
CsiI	ACCWGGT	1 cut(s)	1717
Csp6I	GTAC	7 cut(s)	623, 994, 1224, 1584, 1623, 1944, 2367
CspCI	CAANNNNNGTGG	2 cut(s)	2440, 2475
CviQI	GTAC	7 cut(s)	623, 994, 1224, 1584, 1623, 1944, 2367
DraIII	CACNNNGTG	1 cut(s)	1214
Eam1104I	CTCTTC	3 cut(s)	762, 1954, 2428
EarI	CTCTTC	3 cut(s)	762, 1954, 2428
EciI	GGCGGA	1 cut(s)	678
Ecl136II	GAGCTC	1 cut(s)	1899
Eco147I	AGGCCT	1 cut(s)	1081
Eco24I	GRGCYC	1 cut(s)	1901
Eco31I	GGTCTC	1 cut(s)	1873
Eco47I	GGWCC	2 cut(s)	400, 2821
Eco47III	AGCGCT	1 cut(s)	24
Eco53kI	GAGCTC	1 cut(s)	1899
Eco57I	CTGAAG	2 cut(s)	942, 1227
Eco81I	CCTNAGG	1 cut(s)	2028
EcoICRI	GAGCTC	1 cut(s)	1899
EcoNI	CCTNNNNNAGG	3 cut(s)	888, 1134, 2027
EcoO109I	RGGNCCY	1 cut(s)	400
EcoRI	GAATTC	1 cut(s)	1143
EcoRII	CCWGG	6 cut(s)	565, 658, 731, 1588, 1717, 2248
EcoT22I	ATGCAT	1 cut(s)	949
EcoT38I	GRGCYC	1 cut(s)	1901
Esp3I	CGTCTC	1 cut(s)	144
FalI	AAGNNNNNCTT	4 cut(s)	9, 41, 1428, 1460
FaqI	GGGAC	4 cut(s)	1288, 1487, 1940, 2731
FauNDI	CATATG	1 cut(s)	2379
FbaI	TGATCA	3 cut(s)	1156, 1375, 1540
FblI	GTMKAC	1 cut(s)	856
Fnu4HI	GCNGC	5 cut(s)	82, 333, 700, 1020, 2844
FokI	GGATG	9 cut(s)	213, 375, 570, 642, 917, 1500, 1649, 2732, 2743
FriOI	GRGCYC	1 cut(s)	1901
Fsp4HI	GCNGC	5 cut(s)	82, 333, 700, 1020, 2844
FspBI	CTAG	6 cut(s)	492, 1131, 1365, 1959, 2262, 2555
GlaI	GCGC	2 cut(s)	24, 1878
GluI	GCNGC	5 cut(s)	82, 333, 700, 1020, 2844
GsaI	CCCAGC	1 cut(s)	208
GsuI	CTGGAG	2 cut(s)	1167, 1449
HaeII	RGCGCY	1 cut(s)	26
HaeIII	GGCC	4 cut(s)	894, 1081, 1237, 2869
HapII	CCGG	1 cut(s)	2006
HhaI	GCGC	2 cut(s)	25, 1879
Hin1I	GRCGYC	1 cut(s)	531
Hin6I	GCGC	2 cut(s)	23, 1877
HinP1I	GCGC	2 cut(s)	23, 1877
HincII	GTYRAC	2 cut(s)	746, 857
HindII	GTYRAC	2 cut(s)	746, 857
HindIII	AAGCTT	2 cut(s)	1033, 1442
HpaII	CCGG	1 cut(s)	2006
HphI	GGTGA	6 cut(s)	171, 185, 200, 2553, 2674, 2747
Hpy166II	GTNNAC	9 cut(s)	746, 857, 986, 1523, 1623, 2108, 2462, 2755, 2824
Hpy8I	GTNNAC	9 cut(s)	746, 857, 986, 1523, 1623, 2108, 2462, 2755, 2824
Hpy99I	CGWCG	3 cut(s)	317, 694, 858
HpyCH4III	ACNGT	8 cut(s)	445, 652, 854, 1115, 1820, 2105, 2773, 2816
HpyCH4IV	ACGT	2 cut(s)	531, 2365
HpyF10VI	GCNNNNNNNGC	6 cut(s)	31, 2389, 2501, 2699, 2708, 2843
HpySE526I	ACGT	2 cut(s)	531, 2365
Hsp92I	GRCGYC	1 cut(s)	531
HspAI	GCGC	2 cut(s)	23, 1877
KpnI	GGTACC	1 cut(s)	626
Ksp22I	TGATCA	3 cut(s)	1156, 1375, 1540
LguI	GCTCTTC	1 cut(s)	2428
LmnI	GCTCC	4 cut(s)	970, 1471, 2333, 2764
Lsp1109I	GCAGC	5 cut(s)	68, 319, 711, 1006, 2855
MabI	ACCWGGT	1 cut(s)	1717
MaeI	CTAG	6 cut(s)	492, 1131, 1365, 1959, 2262, 2555
MaeII	ACGT	2 cut(s)	531, 2365
MaeIII	GTNAC	4 cut(s)	2315, 2398, 2517, 2722
MflI	RGATCY	7 cut(s)	1056, 1592, 1735, 1774, 2032, 2129, 2551
MhlI	GDGCHC	3 cut(s)	574, 1901, 2695
MlyI	GAGTC	3 cut(s)	267, 981, 1504
MmeI	TCCRAC	1 cut(s)	304
Mph1103I	ATGCAT	1 cut(s)	949
MroXI	GAANNNNTTC	2 cut(s)	771, 2104
MseI	TTAA	7 cut(s)	27, 101, 273, 525, 1287, 1446, 1683
MslI	CAYNNNNRTG	3 cut(s)	1436, 1518, 1766
MspA1I	CMGCKG	3 cut(s)	431, 1019, 1280
MspCI	CTTAAG	1 cut(s)	26
MspI	CCGG	1 cut(s)	2006
MspR9I	CCNGG	7 cut(s)	567, 660, 733, 1590, 1719, 2007, 2250
Mva1269I	GAATGC	4 cut(s)	240, 441, 645, 1757
MvaI	CCWGG	6 cut(s)	567, 660, 733, 1590, 1719, 2250
MwoI	GCNNNNNNNGC	6 cut(s)	31, 2389, 2501, 2699, 2708, 2843
NciI	CCSGG	1 cut(s)	2007
NdeI	CATATG	1 cut(s)	2379
NlaIV	GGNNCC	2 cut(s)	624, 2782
NmuCI	GTSAC	3 cut(s)	2315, 2398, 2517
NsiI	ATGCAT	1 cut(s)	949
NspI	RCATGY	2 cut(s)	1529, 2411
OliI	CACNNNNGTG	2 cut(s)	1436, 1766
PaeI	GCATGC	1 cut(s)	2411
PagI	TCATGA	3 cut(s)	844, 1119, 1558
PceI	AGGCCT	1 cut(s)	1081
PciI	ACATGT	1 cut(s)	1525
PciSI	GCTCTTC	1 cut(s)	2428
PctI	GAATGC	4 cut(s)	240, 441, 645, 1757
PdmI	GAANNNNTTC	2 cut(s)	771, 2104
PfeI	GAWTC	9 cut(s)	113, 176, 358, 519, 772, 1334, 1555, 2257, 2558
PflMI	CCANNNNNTGG	1 cut(s)	51
PkrI	GCNGC	5 cut(s)	83, 334, 701, 1021, 2845
PleI	GAGTC	3 cut(s)	266, 981, 1504
PpsI	GAGTC	3 cut(s)	266, 981, 1504
PpuMI	RGGWCCY	1 cut(s)	400
PscI	ACATGT	1 cut(s)	1525
PshAI	GACNNNNGTC	1 cut(s)	2792
PshBI	ATTAAT	2 cut(s)	525, 1287
Psp124BI	GAGCTC	1 cut(s)	1901
Psp5II	RGGWCCY	1 cut(s)	400
Psp6I	CCWGG	6 cut(s)	565, 658, 731, 1588, 1717, 2248
PspFI	CCCAGC	1 cut(s)	204
PspGI	CCWGG	6 cut(s)	565, 658, 731, 1588, 1717, 2248
PspN4I	GGNNCC	2 cut(s)	624, 2782
PspPI	GGNCC	3 cut(s)	400, 2821, 2868
PspPPI	RGGWCCY	1 cut(s)	400
PstNI	CAGNNNCTG	2 cut(s)	1595, 1844
PsuI	RGATCY	7 cut(s)	1056, 1592, 1735, 1774, 2032, 2129, 2551
PvuII	CAGCTG	3 cut(s)	431, 1019, 1280
RsaI	GTAC	7 cut(s)	624, 995, 1225, 1585, 1624, 1945, 2368
RsaNI	GTAC	7 cut(s)	623, 994, 1224, 1584, 1623, 1944, 2367
RseI	CAYNNNNRTG	3 cut(s)	1436, 1518, 1766
SacI	GAGCTC	1 cut(s)	1901
SalI	GTCGAC	1 cut(s)	855
SapI	GCTCTTC	1 cut(s)	2428
SaqAI	TTAA	7 cut(s)	27, 101, 273, 525, 1287, 1446, 1683
SatI	GCNGC	5 cut(s)	82, 333, 700, 1020, 2844
Sau96I	GGNCC	3 cut(s)	400, 2821, 2868
ScaI	AGTACT	1 cut(s)	1225
SchI	GAGTC	3 cut(s)	267, 981, 1504
ScrFI	CCNGG	7 cut(s)	567, 660, 733, 1590, 1719, 2007, 2250
SduI	GDGCHC	3 cut(s)	574, 1901, 2695
SexAI	ACCWGGT	1 cut(s)	1717
SfcI	CTRYAG	1 cut(s)	2703
SinI	GGWCC	2 cut(s)	400, 2821
SmiMI	CAYNNNNRTG	3 cut(s)	1436, 1518, 1766
SmlI	CTYRAG	4 cut(s)	26, 786, 989, 2133
SmoI	CTYRAG	4 cut(s)	26, 786, 989, 2133
SphI	GCATGC	1 cut(s)	2411
SseBI	AGGCCT	1 cut(s)	1081
SsiI	CCGC	1 cut(s)	663
SspI	AATATT	2 cut(s)	781, 2739
SspMI	CTAG	6 cut(s)	492, 1131, 1365, 1959, 2262, 2555
SstI	GAGCTC	1 cut(s)	1901
StuI	AGGCCT	1 cut(s)	1081
StyD4I	CCNGG	7 cut(s)	565, 658, 731, 1588, 1717, 2005, 2248
TaaI	ACNGT	8 cut(s)	445, 652, 854, 1115, 1820, 2105, 2773, 2816
TaiI	ACGT	2 cut(s)	534, 2368
TaqI	TCGA	7 cut(s)	142, 856, 1220, 1232, 1535, 1871, 2361
TatI	WGTACW	2 cut(s)	1223, 1622
TfiI	GAWTC	9 cut(s)	113, 176, 358, 519, 772, 1334, 1555, 2257, 2558
Tru1I	TTAA	7 cut(s)	27, 101, 273, 525, 1287, 1446, 1683
Tru9I	TTAA	7 cut(s)	27, 101, 273, 525, 1287, 1446, 1683
TscAI	CASTG	6 cut(s)	46, 56, 870, 1473, 2361, 2878
TseFI	GTSAC	3 cut(s)	2315, 2398, 2517
TseI	GCWGC	5 cut(s)	81, 332, 699, 1019, 2843
Tsp45I	GTSAC	3 cut(s)	2315, 2398, 2517
TspGWI	ACGGA	2 cut(s)	1448, 1496
TspRI	CASTG	6 cut(s)	46, 56, 870, 1473, 2361, 2878
Van91I	CCANNNNNTGG	1 cut(s)	51
Vha464I	CTTAAG	1 cut(s)	26
VpaK11BI	GGWCC	2 cut(s)	400, 2821
VspI	ATTAAT	2 cut(s)	525, 1287
XagI	CCTNNNNNAGG	3 cut(s)	888, 1134, 2027
XapI	RAATTY	5 cut(s)	828, 840, 1143, 1319, 1578
XbaI	TCTAGA	1 cut(s)	2554
XceI	RCATGY	2 cut(s)	1529, 2411
XcmI	CCANNNNNNNNNTGG	3 cut(s)	189, 428, 902
XmiI	GTMKAC	1 cut(s)	856
XmnI	GAANNNNTTC	2 cut(s)	771, 2104
XspI	CTAG	6 cut(s)	492, 1131, 1365, 1959, 2262, 2555
ZraI	GACGTC	1 cut(s)	532
ZrmI	AGTACT	1 cut(s)	1225
Zsp2I	ATGCAT	1 cut(s)	949

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.