Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0003434

Physical Location & Seq

Genomic Coordinates Reverse (-)

23068232 .. 23079972

Gene Structure 11741 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1183000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2751 bp

ATGGTGATGGCTGAGAATGAAGTTGGGGATGACCAGAGGGACCTCAACGTCAATGGCGGCGGGCCCAAGTCCCCATGGAGGACTCCGGTCTCCCTTGATGCTCCCGTAATGGCCGCCGAATCCTGGCCTGCTCTGGCCGATGCCCACAGGCCCAAGAGTCTGGATGCTGCTGCAAAGCCGCCAGCTGCGCAGCCGTCTCCCCTGCCCGCGCCTGCGCAGGGATTAGTCATGCAGAAGTTGAATGAATCTGGAAACTCTAACGCTTCGCACAAGCATTCATCATCACATCACGAAAAAGGTCCTAGGCGCAACACAAATGCTGCACCTCTTTTTCCAGTACCCGTACCCTACCATCAACAATCGCTTCCTACTGTTTTTCATACCATGGTACAACCCTACATTCCGGCTTCTGGGTATGCTTACCAGCCATACGCTGGACCCATTCCAAGTGTTGGGAATCATATGGTAAAATCTGGATTCGAGACCCCAGTGCAAAATTTTGTGCAACCAGTTCAACCTCAGCCACGAAATAATCCGAATGCTTATGGTGTCAATTTTTCTACTAGAAGACCCAATATGCAAGAACCCGGTGGCCCTTGGAATCATACTTGGCATCAACAGCGAGCATTCAACCCCAGAGAGAACATTCCTGTGCAACAGGGTGTTGGACCTAGGCCTTTCTTAAGACCTCAGCTTTTTGGTCCAGCCCCAAGGTTCATGGTTGGCCCAAGCATTCCTGGACCTGCACCCATATGCTATCTTCCAGTTCCACCGCTTTCCGCAATTAGGGGACCTCATCCTCCTAGCTTTGTGCCACATCCTTTAAATCCAGGGGCTCCATTACCGCCCTCAAAAACACATACATTGTCGTTGAGGGACAATATTATAAAGCAAATAGAGTATTATTTCAGTGATGAAAATCTAAAGAAAGACCGTTACTTAATTTCATTCATGGATGACCAAGGATGGGTCCCAATAACCACCATTGCTGAATTTAAAAGGGTTAAGAAAATGTGTACAGACATAGCATTCATCATTGACTCGCTGCTGGGTTCAACTACTGTAGAAGTGCAGGCTAACAAGATACGAAGACGTGATGAGTGGTCGAAGTGGATTGCAGATTCTGTAGACTCAATGTTAACATCCAAGCTGCAAACTCACATGGTTCAAGTTGAAGATAGATATATCAATGCTCCTGAGAATAGCACGAGCAATGATAGAATGAAAGCTTTCGAAGAAAAGGTAGAAGTTTTATCAGATGGAAAAAAATTGATGTCGTGCATGCCATCAACCACCATACCTGGCATTGATGATGTTCAAGTTGATAGCGGATCACTAGCATCTGCTGATTATAATAGTGCTTTAAGTGCAAAGTTGACTTCCAAATCAAATTGCAAGTCCTCAGATCCCGAAATGGATAATTACTTAGACCGCCCATACCACTCAGAGGGAATTGAAACTGTAAGATATGATGATGATGGGACTGAAGGCATGCCTTCAGATATGGATATGAAGAATCTTGCAATGTCTAACGATTTTGCAAACACATTTATGCTTGATGAAGAGCTAGAGCTTGAGCAGAAAATAATAAAGGATGATCATTCTCCTATTAGAAGTGTTGGCCCAAGGATTGATGATGAAGATGATGAGATTCTAGTCAATGATCAGGACGTACAGAGACTTGTAATTGTCACCCAGAATAGTAGAGTAGGTAAAGGATCAAAACATGATGATAAAGAATCAAAAACCATTTCCAATGAGCTAGCGTCTGCTATAAACGATGGGCTTTACTTCTATGAGCAGGAACTGAAAACCAAGCGACCAAACCATAAAAAGTACAGCACCAGCCATGAAAATAGAGATGCAAACTCAAGATTTTCAAGCGTTTCCAAAGTCTTTTCAAAATTAAAGCCCAGCGAAATATCTGGCAGTAGTAATGGCATTGAAGAGTCTGGAAGTGTTAATGCACGGAAGAAGCAAAATAGACTCTCCCAAAATCAGCAGCCGTCCCATAGACAGCGATTCTTCTCAAGCAACTTCAAAAATTATGGAACTGGTTGGAACAATCTAGGAATCATATCAGAAAGTCCACCAAGTCACTCTGTTGGCTTTTTCTTTAGTTCCACGCCTCCTGAAACTCATGGACCAAGATCTTCAAAAGTGAGAGTTTCCCCTCATGGGTTTCTCTCAGGCAGTAGTCCACCGGTGGGTTCTGTGCCAAAGTCATTTCCACCGTTTCAGCACCCAAGTCATCAGCTTCTGGAAGAAAATGGTTTTAAACAGCAGAAGTACCTCAAGTATCATAACCGGTGCCTAAATGACAGGAAGAAGTTGGGAATTGGTTGCAGTGAGGAAATGAATACTCTGTATAGATTTTGGTGTTATTTTCTTAGAAACATGTTCAACTCTTCAATGTATGATGAGTTCCGAAATTATGCACTTGAAGATGCTACTGCTGGTTATAATTATGGAGTAGAGTGCCTCTTCAGGTTCTATAGTTATGGTTTGGAGAAAGATTTTAGAGAGGACTTGTACAAGGACTTTGAACAGCTCACTCTTGACTTTTACCATAAAGGCAATCTCTATGGCCTGGAAAAATATTGGGCTTTTCATCATTATCGTCAACAAGGGGATCAGAAAGAGTCTCTAATGAAACATTCGGAATTGGATCGGTTGCTCAGGGAAGTATACTGTCGCTTGGAGGACTTCCGTGCCAAAGAGAGCCACTGCCATGAAGAGAGGAGTCATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000932 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005844 GO:0006139 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009266 GO:0009408 GO:0009628 GO:0009892 GO:0009987 GO:0010286 GO:0010468 GO:0010605 GO:0010629 GO:0016070 GO:0016071 GO:0019222 GO:0019439 GO:0032991 GO:0034641 GO:0034655 GO:0035770 GO:0036464 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046700 GO:0048519 GO:0050789 GO:0050896 GO:0060255 GO:0065007 GO:0071704 GO:0090304 GO:1901360 GO:1901361 GO:1901575 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Protein Analysis

917

Amino Acids

103.63

Weight (kDa)

6.64

Isoelectric Point (pI)

50.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
La	PF05383	295 - 349	1.1e-23	La domain
LARP1_HEAT	PF21071	748 - 893	1.5e-60	LARP1 HEAT repeat region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003434)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G21160 AT5G21160 AT5G21160
fragaria_vesca	FvH4_3g27710 FvH4_3g27710 FvH4_3g27710 FvH4_3g27710 FvH4_3g27710 FvH4_3g27710 FvH4_3g27710 FvH4_3g27710 FvH4_3g27710 FvH4_3g27710 FvH4_3g27710 FvH4_3g27710 FvH4_3g27710 FvH4_3g27710 FvH4_3g27710 FvH4_3g27710
malus_domestica	MD00G1106400.v1.1 MD11G1183000.v1.1
prunus_persica	Prupe.6G155800_v2.0.a1 Prupe.6G155800_v2.0.a1 Prupe.6G155800_v2.0.a1 Prupe.6G155800_v2.0.a1
pyrus_communis	pycom5153684g00350
rosa_chinensis	RchiOBHm_Chr5g0050001
rosa_laevigata	RLG00000034676
rosa_multiflora	Rmu_sc0000333.1_g000023
rosa_roxburghii	Rroxscaffold_1G00031150
rosa_rugosa	Rorug05G0256500
rosa_samantha	Rh5AG329600 Rh5BG340300 Rh5CG366300 Rh5DG352900
rosa_wichuraiana	Rw5G031120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	887, 1353, 2463
AasI	GACNNNNNNGTC	1 cut(s)	47
Acc16I	TGCGCA	2 cut(s)	189, 216
Acc36I	ACCTGC	1 cut(s)	751
AccB1I	GGYRCC	1 cut(s)	2310
AccB7I	CCANNNNNTGG	2 cut(s)	434, 452
AccI	GTMKAC	2 cut(s)	1128, 2688
AccII	CGCG	1 cut(s)	209
AclWI	GGATC	5 cut(s)	1339, 1400, 1726, 2640, 2676
AcoI	YGGCCR	2 cut(s)	111, 135
AcsI	RAATTY	2 cut(s)	496, 992
AcuI	CTGAAG	3 cut(s)	1482, 1506, 2470
AfaI	GTAC	8 cut(s)	339, 345, 390, 1018, 1674, 1838, 2291, 2534
AflII	CTTAAG	1 cut(s)	682
AflIII	ACRYGT	1 cut(s)	2397
AgeI	ACCGGT	2 cut(s)	2203, 2307
AjiI	CACGTC	1 cut(s)	1094
AjnI	CCWGG	5 cut(s)	122, 736, 829, 1300, 2589
Alw26I	GTCTC	5 cut(s)	94, 201, 476, 1672, 2649
AlwI	GGATC	5 cut(s)	1339, 1400, 1726, 2640, 2676
AlwNI	CAGNNNCTG	3 cut(s)	1124, 1807, 2260
ApaI	GGGCCC	1 cut(s)	66
ApeKI	GCWGC	8 cut(s)	167, 170, 185, 190, 320, 1045, 1150, 2002
ApoI	RAATTY	2 cut(s)	496, 992
ArsI	GACNNNNNNTTYG	2 cut(s)	1382, 1414
AsiGI	ACCGGT	2 cut(s)	2203, 2307
Asp700I	GAANNNNTTC	1 cut(s)	1229
AspA2I	CCTAGG	2 cut(s)	302, 671
AspLEI	GCGC	4 cut(s)	190, 211, 217, 309
AsuC2I	CCSGG	1 cut(s)	588
AsuHPI	GGTGA	2 cut(s)	16, 1684
AsuII	TTCGAA	1 cut(s)	1233
AsuNHI	GCTAGC	1 cut(s)	1762
AvaII	GGWCC	9 cut(s)	40, 299, 437, 668, 701, 740, 791, 970, 2144
AvrII	CCTAGG	2 cut(s)	302, 671
BaeGI	GKGCMC	1 cut(s)	66
BanI	GGYRCC	1 cut(s)	2310
BanII	GRGCYC	2 cut(s)	66, 838
BauI	CACGAG	1 cut(s)	1207
BbsI	GAAGAC	2 cut(s)	574, 1096
BbvCI	CCTCAGC	2 cut(s)	519, 690
BbvI	GCAGC	8 cut(s)	154, 157, 172, 202, 307, 1032, 1137, 2014
BccI	CCATC	6 cut(s)	360, 960, 1253, 1294, 1472, 1775
BceAI	ACGGC	2 cut(s)	178, 1990
BciT130I	CCWGG	5 cut(s)	124, 738, 831, 1302, 2591
BclI	TGATCA	2 cut(s)	1597, 1663
BcnI	CCSGG	1 cut(s)	588
BcoDI	GTCTC	5 cut(s)	94, 201, 476, 1672, 2649
BfaI	CTAG	9 cut(s)	303, 564, 672, 804, 1337, 1568, 1655, 1763, 2069
BfmI	CTRYAG	3 cut(s)	1062, 1125, 2494
BfrI	CTTAAG	1 cut(s)	682
BfuAI	ACCTGC	1 cut(s)	751
BglII	AGATCT	1 cut(s)	2150
BlnI	CCTAGG	2 cut(s)	302, 671
Bme1390I	CCNGG	6 cut(s)	124, 588, 738, 831, 1302, 2591
Bme18I	GGWCC	9 cut(s)	40, 299, 437, 668, 701, 740, 791, 970, 2144
BmgBI	CACGTC	1 cut(s)	1094
BmiI	GGNNCC	8 cut(s)	41, 64, 439, 792, 837, 971, 972, 2312
BmrFI	CCNGG	6 cut(s)	124, 588, 738, 831, 1302, 2591
BmrI	ACTGGG	1 cut(s)	482
BmsI	GCATC	7 cut(s)	88, 130, 154, 622, 1349, 1852, 2437
BmtI	GCTAGC	1 cut(s)	1766
BmuI	ACTGGG	1 cut(s)	482
BoxI	GACNNNNGTC	1 cut(s)	86
BpiI	GAAGAC	2 cut(s)	574, 1096
Bpu10I	CCTNAGC	3 cut(s)	519, 690, 2678
Bpu14I	TTCGAA	1 cut(s)	1233
BpuEI	CTTGAG	4 cut(s)	1595, 1855, 2014, 2279
BpuMI	CCSGG	1 cut(s)	588
BsaBI	GATNNNNATC	1 cut(s)	918
BsaI	GGTCTC	2 cut(s)	94, 476
BsaJI	CCNNGG	9 cut(s)	74, 302, 384, 596, 671, 710, 830, 961, 1625
BsaWI	WCCGGW	3 cut(s)	85, 2203, 2307
Bse118I	RCCGGY	2 cut(s)	2203, 2307
Bse1I	ACTGG	5 cut(s)	335, 488, 509, 764, 2059
Bse3DI	GCAATG	3 cut(s)	984, 1219, 1530
Bse8I	GATNNNNATC	1 cut(s)	918
BseBI	CCWGG	5 cut(s)	124, 738, 831, 1302, 2591
BseDI	CCNNGG	9 cut(s)	74, 302, 384, 596, 671, 710, 830, 961, 1625
BseGI	GGATG	8 cut(s)	34, 169, 796, 817, 961, 971, 1142, 1600
BseJI	GATNNNNATC	1 cut(s)	918
BseMI	GCAATG	3 cut(s)	984, 1219, 1530
BseMII	CTCAG	7 cut(s)	533, 704, 1188, 1416, 1458, 2202, 2692
BseNI	ACTGG	5 cut(s)	335, 488, 509, 764, 2059
BseSI	GKGCMC	1 cut(s)	66
BseXI	GCAGC	8 cut(s)	154, 157, 172, 202, 307, 1032, 1137, 2014
BseYI	CCCAGC	2 cut(s)	1048, 1913
BsgI	GTGCAG	3 cut(s)	306, 729, 1091
Bsh1236I	CGCG	1 cut(s)	209
BshNI	GGYRCC	1 cut(s)	2310
BshTI	ACCGGT	2 cut(s)	2203, 2307
BsiSI	CCGG	5 cut(s)	86, 404, 588, 2204, 2308
BslFI	GGGAC	7 cut(s)	53, 55, 804, 890, 956, 1495, 1993
BsmAI	GTCTC	5 cut(s)	94, 201, 476, 1672, 2649
BsmBI	CGTCTC	1 cut(s)	201
BsmFI	GGGAC	7 cut(s)	53, 55, 804, 890, 956, 1495, 1993
BsmI	GAATGC	5 cut(s)	274, 544, 626, 732, 1028
Bso31I	GGTCTC	2 cut(s)	94, 476
Bsp119I	TTCGAA	1 cut(s)	1233
Bsp120I	GGGCCC	1 cut(s)	62
Bsp1286I	GDGCHC	2 cut(s)	66, 838
Bsp1407I	TGTACA	2 cut(s)	1016, 2532
Bsp143I	GATC	8 cut(s)	1331, 1405, 1597, 1663, 1718, 2150, 2632, 2668
Bsp19I	CCATGG	2 cut(s)	74, 384
BspCNI	CTCAG	8 cut(s)	4, 532, 703, 1189, 1415, 1457, 2201, 2691
BspFNI	CGCG	1 cut(s)	209
BspLI	GGNNCC	8 cut(s)	41, 64, 439, 792, 837, 971, 972, 2312
BspMI	ACCTGC	1 cut(s)	751
BspOI	GCTAGC	1 cut(s)	1766
BspPI	GGATC	5 cut(s)	1339, 1400, 1726, 2640, 2676
BspQI	GCTCTTC	1 cut(s)	1557
BspT104I	TTCGAA	1 cut(s)	1233
BspT107I	GGYRCC	1 cut(s)	2310
BspTI	CTTAAG	1 cut(s)	682
BspTNI	GGTCTC	2 cut(s)	94, 476
BsrDI	GCAATG	3 cut(s)	984, 1219, 1530
BsrFI	RCCGGY	2 cut(s)	2203, 2307
BsrGI	TGTACA	2 cut(s)	1016, 2532
BsrI	ACTGG	5 cut(s)	335, 488, 509, 764, 2059
BssAI	RCCGGY	2 cut(s)	2203, 2307
BssECI	CCNNGG	9 cut(s)	74, 302, 384, 596, 671, 710, 830, 961, 1625
BssMI	GATC	8 cut(s)	1331, 1405, 1597, 1663, 1718, 2150, 2632, 2668
BssNAI	GTATAC	1 cut(s)	2689
BssSI	CACGAG	1 cut(s)	1207
BssT1I	CCWWGG	8 cut(s)	74, 302, 384, 596, 671, 710, 961, 1625
Bst1107I	GTATAC	1 cut(s)	2689
Bst2BI	CACGAG	1 cut(s)	1207
Bst2UI	CCWGG	5 cut(s)	124, 738, 831, 1302, 2591
Bst4CI	ACNGT	6 cut(s)	373, 935, 1063, 1462, 2235, 2693
Bst6I	CTCTTC	5 cut(s)	1557, 1941, 2413, 2489, 2730
BstAFI	CTTAAG	1 cut(s)	682
BstAUI	TGTACA	2 cut(s)	1016, 2532
BstBI	TTCGAA	1 cut(s)	1233
BstDSI	CCRYGG	2 cut(s)	74, 384
BstF5I	GGATG	8 cut(s)	34, 169, 796, 817, 961, 971, 1142, 1600
BstFNI	CGCG	1 cut(s)	209
BstHHI	GCGC	4 cut(s)	190, 211, 217, 309
BstKTI	GATC	8 cut(s)	1334, 1408, 1600, 1666, 1721, 2153, 2635, 2671
BstMAI	GTCTC	5 cut(s)	94, 201, 476, 1672, 2649
BstMBI	GATC	8 cut(s)	1331, 1405, 1597, 1663, 1718, 2150, 2632, 2668
BstMWI	GCNNNNNNNGC	3 cut(s)	187, 619, 1367
BstNI	CCWGG	5 cut(s)	124, 738, 831, 1302, 2591
BstNSI	RCATGY	3 cut(s)	1285, 1495, 2401
BstPAI	GACNNNNGTC	1 cut(s)	86
BstSCI	CCNGG	6 cut(s)	122, 586, 736, 829, 1300, 2589
BstSFI	CTRYAG	3 cut(s)	1062, 1125, 2494
BstSLI	GKGCMC	1 cut(s)	66
BstUI	CGCG	1 cut(s)	209
BstV1I	GCAGC	8 cut(s)	154, 157, 172, 202, 307, 1032, 1137, 2014
BstV2I	GAAGAC	2 cut(s)	574, 1096
BstX2I	RGATCY	2 cut(s)	1405, 2150
BstXI	CCANNNNNNTGG	1 cut(s)	160
BstYI	RGATCY	2 cut(s)	1405, 2150
BstZ17I	GTATAC	1 cut(s)	2689
BtgI	CCRYGG	2 cut(s)	74, 384
BtrI	CACGTC	1 cut(s)	1094
BtsCI	GGATG	8 cut(s)	34, 169, 796, 817, 961, 971, 1142, 1600
BtsI	GCAGTG	2 cut(s)	2353, 2725
BtsIMutI	CAGTG	4 cut(s)	495, 916, 2353, 2725
BveI	ACCTGC	1 cut(s)	751
CaiI	CAGNNNCTG	3 cut(s)	1124, 1807, 2260
CfoI	GCGC	4 cut(s)	190, 211, 217, 309
Cfr10I	RCCGGY	2 cut(s)	2203, 2307
CseI	GACGC	1 cut(s)	1755
Csp6I	GTAC	8 cut(s)	338, 344, 389, 1017, 1673, 1837, 2290, 2533
CspAI	ACCGGT	2 cut(s)	2203, 2307
CviQI	GTAC	8 cut(s)	338, 344, 389, 1017, 1673, 1837, 2290, 2533
DpnI	GATC	8 cut(s)	1333, 1407, 1599, 1665, 1720, 2152, 2634, 2670
DpnII	GATC	8 cut(s)	1331, 1405, 1597, 1663, 1718, 2150, 2632, 2668
DraI	TTTAAA	3 cut(s)	825, 997, 2278
DrdI	GACNNNNNNGTC	1 cut(s)	47
DseDI	GACNNNNNNGTC	1 cut(s)	47
EaeI	YGGCCR	2 cut(s)	111, 135
Eam1104I	CTCTTC	5 cut(s)	1557, 1941, 2413, 2489, 2730
EarI	CTCTTC	5 cut(s)	1557, 1941, 2413, 2489, 2730
Eco130I	CCWWGG	8 cut(s)	74, 302, 384, 596, 671, 710, 961, 1625
Eco147I	AGGCCT	1 cut(s)	676
Eco24I	GRGCYC	2 cut(s)	66, 838
Eco31I	GGTCTC	2 cut(s)	94, 476
Eco47I	GGWCC	9 cut(s)	40, 299, 437, 668, 701, 740, 791, 970, 2144
Eco57I	CTGAAG	3 cut(s)	1482, 1506, 2470
EcoO109I	RGGNCCY	4 cut(s)	40, 299, 791, 970
EcoRII	CCWGG	5 cut(s)	122, 736, 829, 1300, 2589
EcoT14I	CCWWGG	8 cut(s)	74, 302, 384, 596, 671, 710, 961, 1625
EcoT38I	GRGCYC	2 cut(s)	66, 838
ErhI	CCWWGG	8 cut(s)	74, 302, 384, 596, 671, 710, 961, 1625
Esp3I	CGTCTC	1 cut(s)	201
FaqI	GGGAC	7 cut(s)	53, 55, 804, 890, 956, 1495, 1993
FauI	CCCGC	2 cut(s)	53, 214
FauNDI	CATATG	2 cut(s)	462, 752
FbaI	TGATCA	2 cut(s)	1597, 1663
FblI	GTMKAC	2 cut(s)	1128, 2688
FokI	GGATG	8 cut(s)	41, 176, 783, 804, 968, 978, 1129, 1607
FriOI	GRGCYC	2 cut(s)	66, 838
FspBI	CTAG	9 cut(s)	303, 564, 672, 804, 1337, 1568, 1655, 1763, 2069
FspI	TGCGCA	2 cut(s)	189, 216
GlaI	GCGC	4 cut(s)	189, 210, 216, 308
GsaI	CCCAGC	2 cut(s)	1052, 1917
HapII	CCGG	5 cut(s)	86, 404, 588, 2204, 2308
HgaI	GACGC	1 cut(s)	1755
HhaI	GCGC	4 cut(s)	190, 211, 217, 309
Hin6I	GCGC	4 cut(s)	188, 209, 215, 307
HinP1I	GCGC	4 cut(s)	188, 209, 215, 307
HincII	GTYRAC	3 cut(s)	1140, 1377, 2624
HindII	GTYRAC	3 cut(s)	1140, 1377, 2624
HindIII	AAGCTT	1 cut(s)	1227
HpaI	GTTAAC	1 cut(s)	1140
HpaII	CCGG	5 cut(s)	86, 404, 588, 2204, 2308
HphI	GGTGA	2 cut(s)	16, 1684
Hpy166II	GTNNAC	8 cut(s)	1017, 1129, 1140, 1377, 2090, 2201, 2624, 2689
Hpy188I	TCNGA	9 cut(s)	537, 1258, 1405, 1447, 1501, 2083, 2429, 2637, 2662
Hpy8I	GTNNAC	8 cut(s)	1017, 1129, 1140, 1377, 2090, 2201, 2624, 2689
HpyAV	CCTTC	2 cut(s)	1481, 1506
HpyCH4III	ACNGT	6 cut(s)	373, 935, 1063, 1462, 2235, 2693
HpyCH4IV	ACGT	3 cut(s)	48, 1093, 1671
HpyF10VI	GCNNNNNNNGC	3 cut(s)	187, 619, 1367
HpySE526I	ACGT	3 cut(s)	48, 1093, 1671
HspAI	GCGC	4 cut(s)	188, 209, 215, 307
KflI	GGGWCCC	1 cut(s)	970
Ksp22I	TGATCA	2 cut(s)	1597, 1663
KspAI	GTTAAC	1 cut(s)	1140
Kzo9I	GATC	8 cut(s)	1331, 1405, 1597, 1663, 1718, 2150, 2632, 2668
LguI	GCTCTTC	1 cut(s)	1557
LmnI	GCTCC	3 cut(s)	106, 841, 1198
Lsp1109I	GCAGC	8 cut(s)	154, 157, 172, 202, 307, 1032, 1137, 2014
LweI	GCATC	7 cut(s)	88, 130, 154, 622, 1349, 1852, 2437
MaeI	CTAG	9 cut(s)	303, 564, 672, 804, 1337, 1568, 1655, 1763, 2069
MaeII	ACGT	3 cut(s)	48, 1093, 1671
MaeIII	GTNAC	3 cut(s)	935, 1690, 2096
MalI	GATC	8 cut(s)	1333, 1407, 1599, 1665, 1720, 2152, 2634, 2670
MboI	GATC	8 cut(s)	1331, 1405, 1597, 1663, 1718, 2150, 2632, 2668
MflI	RGATCY	2 cut(s)	1405, 2150
MhlI	GDGCHC	2 cut(s)	66, 838
MlyI	GAGTC	7 cut(s)	76, 166, 1034, 1124, 1958, 1980, 2651
MmeI	TCCRAC	2 cut(s)	646, 2039
MroXI	GAANNNNTTC	1 cut(s)	1229
MslI	CAYNNNNRTG	4 cut(s)	650, 751, 1550, 2730
MspA1I	CMGCKG	1 cut(s)	185
MspCI	CTTAAG	1 cut(s)	682
MspI	CCGG	5 cut(s)	86, 404, 588, 2204, 2308
MspR9I	CCNGG	6 cut(s)	124, 588, 738, 831, 1302, 2591
Mva1269I	GAATGC	5 cut(s)	274, 544, 626, 732, 1028
MvaI	CCWGG	5 cut(s)	124, 738, 831, 1302, 2591
MvnI	CGCG	1 cut(s)	209
MwoI	GCNNNNNNNGC	3 cut(s)	187, 619, 1367
NciI	CCSGG	1 cut(s)	588
NcoI	CCATGG	2 cut(s)	74, 384
NdeI	CATATG	2 cut(s)	462, 752
NdeII	GATC	8 cut(s)	1331, 1405, 1597, 1663, 1718, 2150, 2632, 2668
NheI	GCTAGC	1 cut(s)	1762
NlaIV	GGNNCC	8 cut(s)	41, 64, 439, 792, 837, 971, 972, 2312
NmuCI	GTSAC	2 cut(s)	1690, 2096
NsbI	TGCGCA	2 cut(s)	189, 216
NspI	RCATGY	3 cut(s)	1285, 1495, 2401
NspV	TTCGAA	1 cut(s)	1233
PaeI	GCATGC	2 cut(s)	1285, 1495
PceI	AGGCCT	1 cut(s)	676
PciI	ACATGT	1 cut(s)	2397
PciSI	GCTCTTC	1 cut(s)	1557
PctI	GAATGC	5 cut(s)	274, 544, 626, 732, 1028
PdmI	GAANNNNTTC	1 cut(s)	1229
PflMI	CCANNNNNTGG	2 cut(s)	434, 452
PfoI	TCCNGGA	1 cut(s)	736
PinAI	ACCGGT	2 cut(s)	2203, 2307
PleI	GAGTC	7 cut(s)	76, 165, 1034, 1124, 1957, 1980, 2650
PpsI	GAGTC	7 cut(s)	76, 165, 1034, 1124, 1957, 1980, 2650
PpuMI	RGGWCCY	4 cut(s)	40, 299, 791, 970
PscI	ACATGT	1 cut(s)	2397
PshAI	GACNNNNGTC	1 cut(s)	86
PsiI	TTATAA	3 cut(s)	887, 1353, 2463
Psp5II	RGGWCCY	4 cut(s)	40, 299, 791, 970
Psp6I	CCWGG	5 cut(s)	122, 736, 829, 1300, 2589
PspFI	CCCAGC	2 cut(s)	1048, 1913
PspGI	CCWGG	5 cut(s)	122, 736, 829, 1300, 2589
PspN4I	GGNNCC	8 cut(s)	41, 64, 439, 792, 837, 971, 972, 2312
PspOMI	GGGCCC	1 cut(s)	62
PspPPI	RGGWCCY	4 cut(s)	40, 299, 791, 970
PsrI	GAACNNNNNNTAC	2 cut(s)	2408, 2440
PstNI	CAGNNNCTG	3 cut(s)	1124, 1807, 2260
PsuI	RGATCY	2 cut(s)	1405, 2150
PvuII	CAGCTG	1 cut(s)	185
RsaI	GTAC	8 cut(s)	339, 345, 390, 1018, 1674, 1838, 2291, 2534
RsaNI	GTAC	8 cut(s)	338, 344, 389, 1017, 1673, 1837, 2290, 2533
RseI	CAYNNNNRTG	4 cut(s)	650, 751, 1550, 2730
SapI	GCTCTTC	1 cut(s)	1557
Sau3AI	GATC	8 cut(s)	1331, 1405, 1597, 1663, 1718, 2150, 2632, 2668
SchI	GAGTC	7 cut(s)	76, 166, 1034, 1124, 1958, 1980, 2651
ScrFI	CCNGG	6 cut(s)	124, 588, 738, 831, 1302, 2591
SduI	GDGCHC	2 cut(s)	66, 838
SfaNI	GCATC	7 cut(s)	88, 130, 154, 622, 1349, 1852, 2437
SfcI	CTRYAG	3 cut(s)	1062, 1125, 2494
SfuI	TTCGAA	1 cut(s)	1233
SgrAI	CRCCGGYG	1 cut(s)	2203
SinI	GGWCC	9 cut(s)	40, 299, 437, 668, 701, 740, 791, 970, 2144
SmiMI	CAYNNNNRTG	4 cut(s)	650, 751, 1550, 2730
SmlI	CTYRAG	5 cut(s)	682, 1574, 1870, 2029, 2294
SmoI	CTYRAG	5 cut(s)	682, 1574, 1870, 2029, 2294
SphI	GCATGC	2 cut(s)	1285, 1495
SseBI	AGGCCT	1 cut(s)	676
SspI	AATATT	2 cut(s)	883, 2600
SspMI	CTAG	9 cut(s)	303, 564, 672, 804, 1337, 1568, 1655, 1763, 2069
StuI	AGGCCT	1 cut(s)	676
StyD4I	CCNGG	6 cut(s)	122, 586, 736, 829, 1300, 2589
StyI	CCWWGG	8 cut(s)	74, 302, 384, 596, 671, 710, 961, 1625
TaaI	ACNGT	6 cut(s)	373, 935, 1063, 1462, 2235, 2693
TaiI	ACGT	3 cut(s)	51, 1096, 1674
TaqI	TCGA	3 cut(s)	480, 1106, 1233
TatI	WGTACW	3 cut(s)	1016, 1836, 2532
TauI	GCSGC	3 cut(s)	60, 116, 181
TscAI	CASTG	4 cut(s)	495, 916, 2353, 2732
TseFI	GTSAC	2 cut(s)	1690, 2096
TseI	GCWGC	8 cut(s)	167, 170, 185, 190, 320, 1045, 1150, 2002
Tsp45I	GTSAC	2 cut(s)	1690, 2096
TspGWI	ACGGA	2 cut(s)	1984, 2699
TspRI	CASTG	4 cut(s)	495, 916, 2353, 2732
Van91I	CCANNNNNTGG	2 cut(s)	434, 452
Vha464I	CTTAAG	1 cut(s)	682
VpaK11BI	GGWCC	9 cut(s)	40, 299, 437, 668, 701, 740, 791, 970, 2144
XapI	RAATTY	2 cut(s)	496, 992
XceI	RCATGY	3 cut(s)	1285, 1495, 2401
XcmI	CCANNNNNNNNNTGG	1 cut(s)	431
XmaJI	CCTAGG	2 cut(s)	302, 671
XmiI	GTMKAC	2 cut(s)	1128, 2688
XmnI	GAANNNNTTC	1 cut(s)	1229
XspI	CTAG	9 cut(s)	303, 564, 672, 804, 1337, 1568, 1655, 1763, 2069

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.