MD11G1192300.v1.1

translocation protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0011885
Physical Location & Seq
Forward (+)
27326980 .. 27332465
5486 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1192300.v1.1.491

Sequence Viewer

Length: 1128 bp
ATGAAGAAAGCAGGAGGGGCAGAGAAGAAAAGGGTGCGAAGGTCATCGGGCGCTATTCAAAACGGCGGCAGAGATTCCAGCTCCGATACTCCTCCCAGGAAACAAGCTACCCAGAAAGATGTGTTCCAGTTGTTTGCTGAGAAGGTGAGAGACAATAAGGATTTGGTGTCTAGGTGGGCTGTTCTACAGGAGACACGCGTGGAATATTTCAGGGGAAAGGATTTTGTATGCTTTTTGAGAAATCATCCTGAGGTCAAAGATATTCTAGAATCAGATAGAAATCTAGAAGCTGAAGATATTGCCGATGCTTTACTGGGAAAGAATCTTCTAGTACGCTGTGATCGTGTGGTGAAAACTCTTCGTCCTGGGAAGAGAAAGTTGTCTACATGGCCTGCACATTTGGAGATCTTTCCAGACCAAGTATTTTCTGAGAACGACGCCTTCTTTGCATGGACTTTTGTTAAACGGAGGCCTCTCTGGCAGACACTTCTCTCATTTTTCTGGCCAGTGGTTACATTGGCAATTTGTTTGTTTCCTGTATATCCCCATCGGTGCAAGCTTCTAATACTCTACTCATGTCTCGGTGTCCTCCTACTTTTTCTTTCTCTACTAGTTATGAGAGGTGCAATATTTGGAGTGCTATATATTGTTCTGGGAAAGCGAGTTTGGTTTTTCCCGAACATTCTCGCTGAGGAAGCTACTTTGAGAGAACTATTCCGTTTCTGGCCAAAGAAGGATGAAGAGGAGCGACCAAAGTGGACAACAAGGGTTTTCTATGCAGCAGTTGCAGTTCTGGTCATATTGCTGCTGAGGCACCATGCTCCTGATGAAGCTGCTAGAGCCAGGTACCAGAAACGTGTATCTAACATTATCGACGATGTTCTCGAGTGGTCTCCAAGATTGGCGCTTTCTGGGATGATGGAGAATATGCAAACTGTGGTTAACTCGACGGAGCCAAGTAATGCAACTGAGGAAGAGGTGACGCTGCCTGACGCCATGGATGCAGAAACTTTCACGGTGCTGTACGATGCAGAAGTTAGTGAAAATCTAGAGAACAACGACGAAAACGTAGACAGTAGAGAAGAAAACCTAGACAAGAGTGATGAACAGAAACATGATGACATATAA

Protein Analysis

376

Amino Acids

43.35

Weight (kDa)

6.0

Isoelectric Point (pI)

48.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec62 PF03839 124 - 230 4.6e-14 Translocation protein Sec62
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011885)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G20920 AT3G20920
fragaria_vesca FvH4_3g28770
malus_domestica MD03G1174500.v1.1 MD11G1192300.v1.1
prunus_persica Prupe.4G250000_v2.0.a1
pyrus_communis pycom03g13190 pycom11g16810
rosa_chinensis RchiOBHm_Chr5g0052271
rosa_laevigata RLG00000034829
rosa_multiflora Rmu_sc0009513.1_g000001
rosa_roxburghii Rroxscaffold_1G00028110
rosa_rugosa Rorug05G0273200
rosa_samantha Rh5AG346100 Rh5BG356800 Rh5CG382100 Rh5DG372100
rosa_wichuraiana Rw5G032640 Rw5G032680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 846
AccB1I GGYRCC 2 cut(s) 813, 846
AccI GTMKAC 2 cut(s) 383, 1071
AccII CGCG 1 cut(s) 198
AciI CCGC 1 cut(s) 66
AcoI YGGCCR 2 cut(s) 503, 725
AcuI CTGAAG 1 cut(s) 312
AcyI GRCGYC 2 cut(s) 438, 993
AfaI GTAC 3 cut(s) 333, 848, 1025
AflIII ACRYGT 2 cut(s) 196, 856
AgsI TTSAA 1 cut(s) 59
AhlI ACTAGT 1 cut(s) 610
AjnI CCWGG 3 cut(s) 95, 364, 842
AjuI GAANNNNNNNTTGG 2 cut(s) 649, 681
AluBI AGCT 6 cut(s) 81, 107, 290, 559, 698, 833
AluI AGCT 6 cut(s) 81, 107, 290, 559, 698, 833
Alw26I GTCTC 4 cut(s) 144, 185, 584, 897
Ama87I CYCGRG 1 cut(s) 884
AoxI GGCC 4 cut(s) 389, 470, 503, 725
ApeKI GCWGC 4 cut(s) 779, 805, 833, 985
ArsI GACNNNNNNTTYG 2 cut(s) 428, 460
Asp718I GGTACC 1 cut(s) 846
AspLEI GCGC 2 cut(s) 53, 907
AsuHPI GGTGA 3 cut(s) 157, 361, 991
AvaI CYCGRG 1 cut(s) 884
AxyI CCTNAGG 1 cut(s) 249
BalI TGGCCA 2 cut(s) 505, 727
BanI GGYRCC 2 cut(s) 813, 846
BbvCI CCTCAGC 2 cut(s) 690, 809
BbvI GCAGC 4 cut(s) 791, 792, 820, 972
BccI CCATC 2 cut(s) 555, 913
BceAI ACGGC 1 cut(s) 79
BciT130I CCWGG 3 cut(s) 97, 366, 844
BcoDI GTCTC 4 cut(s) 144, 185, 584, 897
BcuI ACTAGT 1 cut(s) 610
BfaI CTAG 8 cut(s) 171, 266, 284, 329, 611, 837, 1049, 1091
BfmI CTRYAG 1 cut(s) 185
BfoI RGCGCY 2 cut(s) 54, 908
BglI GCCNNNNNGGC 1 cut(s) 478
BglII AGATCT 1 cut(s) 405
BisI GCNGC 5 cut(s) 67, 780, 806, 834, 986
BlsI GCNGC 5 cut(s) 68, 781, 807, 835, 987
Bme1390I CCNGG 3 cut(s) 97, 366, 844
BmeT110I CYCGRG 1 cut(s) 884
BmiI GGNNCC 3 cut(s) 815, 848, 954
BmrFI CCNGG 3 cut(s) 97, 366, 844
BmrI ACTGGG 1 cut(s) 323
BmsI GCATC 3 cut(s) 295, 991, 1018
BmuI ACTGGG 1 cut(s) 323
Bpu10I CCTNAGC 2 cut(s) 690, 809
BsaBI GATNNNNATC 1 cut(s) 279
BsaHI GRCGYC 2 cut(s) 438, 993
BsaI GGTCTC 1 cut(s) 897
BsaJI CCNNGG 3 cut(s) 95, 365, 996
Bse1I ACTGG 3 cut(s) 127, 318, 506
Bse21I CCTNAGG 1 cut(s) 249
Bse8I GATNNNNATC 1 cut(s) 279
BseBI CCWGG 3 cut(s) 97, 366, 844
BseDI CCNNGG 3 cut(s) 95, 365, 996
BseGI GGATG 4 cut(s) 244, 742, 921, 1006
BseJI GATNNNNATC 1 cut(s) 279
BseMII CTCAG 6 cut(s) 129, 240, 420, 681, 800, 960
BseNI ACTGG 3 cut(s) 127, 318, 506
BseRI GAGGAG 2 cut(s) 81, 758
BseXI GCAGC 4 cut(s) 791, 792, 820, 972
BsgI GTGCAG 1 cut(s) 378
Bsh1236I CGCG 1 cut(s) 198
BshFI GGCC 4 cut(s) 391, 472, 505, 727
BshNI GGYRCC 2 cut(s) 813, 846
BsiHKCI CYCGRG 1 cut(s) 884
BsmAI GTCTC 4 cut(s) 144, 185, 584, 897
BsnI GGCC 4 cut(s) 391, 472, 505, 727
Bso31I GGTCTC 1 cut(s) 897
BsoBI CYCGRG 1 cut(s) 884
Bsp143I GATC 2 cut(s) 340, 405
Bsp19I CCATGG 1 cut(s) 996
BspACI CCGC 1 cut(s) 66
BspANI GGCC 4 cut(s) 391, 472, 505, 727
BspCNI CTCAG 6 cut(s) 130, 241, 421, 682, 801, 961
BspFNI CGCG 1 cut(s) 198
BspLI GGNNCC 3 cut(s) 815, 848, 954
BspT107I GGYRCC 2 cut(s) 813, 846
BspTNI GGTCTC 1 cut(s) 897
BsrI ACTGG 3 cut(s) 127, 318, 506
BssECI CCNNGG 3 cut(s) 95, 365, 996
BssMI GATC 2 cut(s) 340, 405
BssNI GRCGYC 2 cut(s) 438, 993
BssT1I CCWWGG 1 cut(s) 996
Bst2UI CCWGG 3 cut(s) 97, 366, 844
Bst4CI ACNGT 3 cut(s) 937, 1018, 1076
Bst6I CTCTTC 4 cut(s) 363, 365, 735, 969
BstACI GRCGYC 2 cut(s) 438, 993
BstAPI GCANNNNNTGC 1 cut(s) 785
BstC8I GCNNGC 2 cut(s) 393, 557
BstDEI CTNAG 6 cut(s) 138, 249, 429, 690, 809, 969
BstDSI CCRYGG 1 cut(s) 996
BstF5I GGATG 4 cut(s) 244, 742, 921, 1006
BstFNI CGCG 1 cut(s) 198
BstH2I RGCGCY 2 cut(s) 54, 908
BstHHI GCGC 2 cut(s) 53, 907
BstKTI GATC 2 cut(s) 343, 408
BstMAI GTCTC 4 cut(s) 144, 185, 584, 897
BstMBI GATC 2 cut(s) 340, 405
BstMWI GCNNNNNNNGC 8 cut(s) 17, 446, 478, 695, 785, 811, 839, 1001
BstNI CCWGG 3 cut(s) 97, 366, 844
BstSCI CCNGG 3 cut(s) 95, 364, 842
BstSFI CTRYAG 1 cut(s) 185
BstUI CGCG 1 cut(s) 198
BstV1I GCAGC 4 cut(s) 791, 792, 820, 972
BstX2I RGATCY 1 cut(s) 405
BstYI RGATCY 1 cut(s) 405
Bsu36I CCTNAGG 1 cut(s) 249
BsuRI GGCC 4 cut(s) 391, 472, 505, 727
BtgI CCRYGG 1 cut(s) 996
BtsCI GGATG 4 cut(s) 244, 742, 921, 1006
BtsIMutI CAGTG 1 cut(s) 513
Cac8I GCNNGC 2 cut(s) 393, 557
CfoI GCGC 2 cut(s) 53, 907
CseI GACGC 3 cut(s) 446, 991, 1001
Csp6I GTAC 3 cut(s) 332, 847, 1024
CviAII CATG 6 cut(s) 387, 450, 576, 818, 997, 1115
CviQI GTAC 3 cut(s) 332, 847, 1024
DdeI CTNAG 6 cut(s) 138, 249, 429, 690, 809, 969
DpnI GATC 2 cut(s) 342, 407
DpnII GATC 2 cut(s) 340, 405
EaeI YGGCCR 2 cut(s) 503, 725
Eam1104I CTCTTC 4 cut(s) 363, 365, 735, 969
EarI CTCTTC 4 cut(s) 363, 365, 735, 969
Eco130I CCWWGG 1 cut(s) 996
Eco147I AGGCCT 1 cut(s) 472
Eco31I GGTCTC 1 cut(s) 897
Eco57I CTGAAG 1 cut(s) 312
Eco81I CCTNAGG 1 cut(s) 249
Eco88I CYCGRG 1 cut(s) 884
EcoRII CCWGG 3 cut(s) 95, 364, 842
EcoT14I CCWWGG 1 cut(s) 996
ErhI CCWWGG 1 cut(s) 996
FaeI CATG 6 cut(s) 390, 453, 579, 821, 1000, 1118
FatI CATG 6 cut(s) 386, 449, 575, 817, 996, 1114
FblI GTMKAC 2 cut(s) 383, 1071
Fnu4HI GCNGC 5 cut(s) 67, 780, 806, 834, 986
FokI GGATG 4 cut(s) 231, 749, 928, 1013
Fsp4HI GCNGC 5 cut(s) 67, 780, 806, 834, 986
FspBI CTAG 8 cut(s) 171, 266, 284, 329, 611, 837, 1049, 1091
GlaI GCGC 2 cut(s) 52, 906
GluI GCNGC 5 cut(s) 67, 780, 806, 834, 986
HaeII RGCGCY 2 cut(s) 54, 908
HaeIII GGCC 4 cut(s) 391, 472, 505, 727
HgaI GACGC 3 cut(s) 446, 991, 1001
HhaI GCGC 2 cut(s) 53, 907
Hin1I GRCGYC 2 cut(s) 438, 993
Hin1II CATG 6 cut(s) 390, 453, 579, 821, 1000, 1118
Hin6I GCGC 2 cut(s) 51, 905
HinP1I GCGC 2 cut(s) 51, 905
HincII GTYRAC 1 cut(s) 943
HindII GTYRAC 1 cut(s) 943
HindIII AAGCTT 1 cut(s) 557
HinfI GANTC 3 cut(s) 74, 269, 322
HpaI GTTAAC 1 cut(s) 943
HphI GGTGA 3 cut(s) 157, 361, 991
Hpy166II GTNNAC 4 cut(s) 384, 759, 943, 1072
Hpy188I TCNGA 3 cut(s) 85, 274, 430
Hpy188III TCNNGA 8 cut(s) 248, 266, 284, 413, 676, 824, 884, 1049
Hpy8I GTNNAC 4 cut(s) 384, 759, 943, 1072
Hpy99I CGWCG 4 cut(s) 440, 878, 952, 1064
HpyAV CCTTC 4 cut(s) 33, 136, 451, 727
HpyCH4III ACNGT 3 cut(s) 937, 1018, 1076
HpyCH4IV ACGT 2 cut(s) 856, 1068
HpyF10VI GCNNNNNNNGC 8 cut(s) 17, 446, 478, 695, 785, 811, 839, 1001
HpyF3I CTNAG 6 cut(s) 138, 249, 429, 690, 809, 969
HpySE526I ACGT 2 cut(s) 856, 1068
Hsp92I GRCGYC 2 cut(s) 438, 993
Hsp92II CATG 6 cut(s) 390, 453, 579, 821, 1000, 1118
HspAI GCGC 2 cut(s) 51, 905
KpnI GGTACC 1 cut(s) 850
KspAI GTTAAC 1 cut(s) 943
Kzo9I GATC 2 cut(s) 340, 405
LmnI GCTCC 4 cut(s) 86, 745, 826, 952
Lsp1109I GCAGC 4 cut(s) 791, 792, 820, 972
LweI GCATC 3 cut(s) 295, 991, 1018
MaeI CTAG 8 cut(s) 171, 266, 284, 329, 611, 837, 1049, 1091
MaeII ACGT 2 cut(s) 856, 1068
MaeIII GTNAC 2 cut(s) 511, 979
MalI GATC 2 cut(s) 342, 407
MboI GATC 2 cut(s) 340, 405
MboII GAAGA 9 cut(s) 16, 37, 305, 317, 350, 382, 752, 986, 1094
MflI RGATCY 1 cut(s) 405
MlsI TGGCCA 2 cut(s) 505, 727
MluCI AATT 1 cut(s) 522
MluI ACGCGT 1 cut(s) 196
MluNI TGGCCA 2 cut(s) 505, 727
Mox20I TGGCCA 2 cut(s) 505, 727
MscI TGGCCA 2 cut(s) 505, 727
MseI TTAA 2 cut(s) 462, 942
Msp20I TGGCCA 2 cut(s) 505, 727
MspR9I CCNGG 3 cut(s) 97, 366, 844
MvaI CCWGG 3 cut(s) 97, 366, 844
MvnI CGCG 1 cut(s) 198
MwoI GCNNNNNNNGC 8 cut(s) 17, 446, 478, 695, 785, 811, 839, 1001
NcoI CCATGG 1 cut(s) 996
NdeII GATC 2 cut(s) 340, 405
NlaIII CATG 6 cut(s) 390, 453, 579, 821, 1000, 1118
NlaIV GGNNCC 3 cut(s) 815, 848, 954
NmuCI GTSAC 1 cut(s) 979
PaeR7I CTCGAG 1 cut(s) 884
PceI AGGCCT 1 cut(s) 472
PcsI WCGNNNNNNNCGW 3 cut(s) 340, 882, 1065
PfeI GAWTC 3 cut(s) 74, 269, 322
PkrI GCNGC 5 cut(s) 68, 781, 807, 835, 987
Psp6I CCWGG 3 cut(s) 95, 364, 842
PspGI CCWGG 3 cut(s) 95, 364, 842
PspN4I GGNNCC 3 cut(s) 815, 848, 954
PsuI RGATCY 1 cut(s) 405
RsaI GTAC 3 cut(s) 333, 848, 1025
RsaNI GTAC 3 cut(s) 332, 847, 1024
SaqAI TTAA 2 cut(s) 462, 942
SatI GCNGC 5 cut(s) 67, 780, 806, 834, 986
Sau3AI GATC 2 cut(s) 340, 405
ScrFI CCNGG 3 cut(s) 97, 366, 844
SfaNI GCATC 3 cut(s) 295, 991, 1018
SfcI CTRYAG 1 cut(s) 185
Sfr274I CTCGAG 1 cut(s) 884
SlaI CTCGAG 1 cut(s) 884
SmlI CTYRAG 1 cut(s) 884
SmoI CTYRAG 1 cut(s) 884
SpeI ACTAGT 1 cut(s) 610
Sse9I AATT 1 cut(s) 522
SseBI AGGCCT 1 cut(s) 472
SsiI CCGC 1 cut(s) 66
SspI AATATT 2 cut(s) 206, 630
SspMI CTAG 8 cut(s) 171, 266, 284, 329, 611, 837, 1049, 1091
StuI AGGCCT 1 cut(s) 472
StyD4I CCNGG 3 cut(s) 95, 364, 842
StyI CCWWGG 1 cut(s) 996
TaaI ACNGT 3 cut(s) 937, 1018, 1076
TaiI ACGT 2 cut(s) 859, 1071
TaqI TCGA 3 cut(s) 873, 885, 947
TasI AATT 1 cut(s) 522
TauI GCSGC 1 cut(s) 69
TfiI GAWTC 3 cut(s) 74, 269, 322
Tru1I TTAA 2 cut(s) 462, 942
Tru9I TTAA 2 cut(s) 462, 942
TscAI CASTG 1 cut(s) 513
TseFI GTSAC 1 cut(s) 979
TseI GCWGC 4 cut(s) 779, 805, 833, 985
Tsp45I GTSAC 1 cut(s) 979
TspDTI ATGAA 4 cut(s) 17, 753, 843, 1119
TspGWI ACGGA 3 cut(s) 481, 707, 965
TspRI CASTG 1 cut(s) 513
XbaI TCTAGA 3 cut(s) 265, 283, 1048
XhoI CTCGAG 1 cut(s) 884
XmiI GTMKAC 2 cut(s) 383, 1071
XspI CTAG 8 cut(s) 171, 266, 284, 329, 611, 837, 1049, 1091
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.