MD11G1210200.v1.1

GDSL esterase lipase At4g26790-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0011664
Physical Location & Seq
Forward (+)
30759529 .. 30761173
1645 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1210200.v1.1.491

Sequence Viewer

Length: 1050 bp
ATGATAGTTAAGGCCACTCAATGGCTCCTCCTAGGTCATCTCCTAGTGCAGGTTTCTATGGTGTGTGCCAGAGTTCCAGCAATCATTGTGTTTGGAGACTCTACTGTGGATGCAGGGAACAACAACCAGATATCGACAGTCCTAAAGAGCAACTTTGAACCCTACGGTCACAATTTCTATGGAGGCTTGGCTACTGGCCGGTTTTCCAACGGTAGGCTTGCAACGGACTTCCTTTCTGAAGGTTTTATGATCAAGCCGACCATACCTGCATACTTGGATCCTGCCTATAGCATTTCGGATTTTGCCACCGGAGTTTGCTTTGCCTCTGCCGGAACTGGCTATGATAATGCTACTTCTGATGTGCTATCTGTGATACCTCTTTGGAAAGAACTGGAGTACTATAAGGAATACCAGAAGAAGCTGCGACGCTATCTAGGCAACGACAAAGCTAACGAGGTTCTGGGAGAAGCACTTTACCTGATTAGTATAGGAACTAATGATTTTCTAGAAAACTACTTTGTATTTTCACGCAGACGATCTCAGTTTTCCATTGAGGAGTACCAAAACTTTCTACTAGGAATTGCTGGAAATTTCATCAGAGAGCTTTACCAGCTTGGAGCTCGGAAGATGGTTATAAGCGGCCTTCCTCCAATGGGTTGCGTGCCACTGGAGAGAACTACAAATATCATTTCTGGGGGTGTTTGTAATGAGAAGTACAATGATGTGGCTAGGAGTTTCAACAAGAAGTTGCAGGGATTGGTTGAAAAGCTCAAGAAGGAACTGGTTAAATTTCAACTTGTTCTTTCAGACCCATACGATATTGTCTCAAAAATCATTCGAAACCCAGCCTTTTTTGGATTTGAAAATGTAGCAAAAGCCTGCTGCGGCACAGGAATGTTCGAGATGAGCTATCTGTGTAATAAGATAAACCCGTTCACATGTTCAGATCCAAATAAATATGTATTCTGGGATGCCTTCCACCCCACAGAGAAAACAAATGCCATTGTTGCAGATCATGTATTTAAAACTGACCTGGTTCAATTTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

350

Amino Acids

39.16

Weight (kDa)

7.51

Isoelectric Point (pI)

26.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 28 - 340 6.6e-34 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011664)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26790 AT4G26790
fragaria_vesca FvH4_3g23490 FvH4_3g23490 FvH4_3g23490
malus_domestica MD11G1210200.v1.1
prunus_persica Prupe.4G222900_v2.0.a1
pyrus_communis pycom11g18580
rosa_chinensis RchiOBHm_Chr5g0041341
rosa_laevigata RLG00000034054
rosa_multiflora Rmu_sc0000594.1_g000072
rosa_roxburghii Rroxscaffold_1G00039290
rosa_rugosa Rorug05G0191600 Rorug05G0191700
rosa_samantha Rh5AG277700 Rh5BG282400 Rh5CG314800 Rh5DG291200
rosa_wichuraiana Rw5G026140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 635
Acc36I ACCTGC 2 cut(s) 40, 274
AccB7I CCANNNNNTGG 1 cut(s) 21
AciI CCGC 2 cut(s) 639, 885
AclWI GGATC 3 cut(s) 272, 285, 941
AcoI YGGCCR 1 cut(s) 196
AcsI RAATTY 2 cut(s) 589, 788
AcuI CTGAAG 1 cut(s) 258
AfaI GTAC 3 cut(s) 398, 560, 716
AfiI CCNNNNNNNGG 4 cut(s) 21, 49, 213, 653
AflIII ACRYGT 1 cut(s) 938
AgsI TTSAA 6 cut(s) 158, 739, 764, 794, 863, 1040
AjnI CCWGG 1 cut(s) 1032
AluBI AGCT 7 cut(s) 421, 449, 604, 613, 620, 769, 909
AluI AGCT 7 cut(s) 421, 449, 604, 613, 620, 769, 909
Alw21I GWGCWC 1 cut(s) 622
Alw26I GTCTC 2 cut(s) 90, 829
AlwI GGATC 3 cut(s) 272, 285, 941
AoxI GGCC 3 cut(s) 12, 196, 640
ApeKI GCWGC 2 cut(s) 421, 882
ApoI RAATTY 2 cut(s) 589, 788
AspA2I CCTAGG 1 cut(s) 31
AsuII TTCGAA 1 cut(s) 838
AvrII CCTAGG 1 cut(s) 31
BamHI GGATCC 1 cut(s) 277
BanII GRGCYC 1 cut(s) 622
Bbv12I GWGCWC 1 cut(s) 622
BbvI GCAGC 2 cut(s) 408, 869
BccI CCATC 1 cut(s) 622
BciT130I CCWGG 1 cut(s) 1034
BclI TGATCA 1 cut(s) 249
BcoDI GTCTC 2 cut(s) 90, 829
BfaI CTAG 6 cut(s) 32, 44, 434, 506, 575, 729
BfmI CTRYAG 1 cut(s) 286
BfuAI ACCTGC 2 cut(s) 40, 274
BisI GCNGC 4 cut(s) 422, 640, 883, 886
BlnI CCTAGG 1 cut(s) 31
BlsI GCNGC 4 cut(s) 423, 641, 884, 887
BmcAI AGTACT 1 cut(s) 398
Bme1390I CCNGG 1 cut(s) 1034
BmiI GGNNCC 2 cut(s) 26, 279
BmrFI CCNGG 1 cut(s) 1034
BmsI GCATC 2 cut(s) 100, 961
BpmI CTGGAG 2 cut(s) 413, 689
Bpu14I TTCGAA 1 cut(s) 838
BpuEI CTTGAG 1 cut(s) 755
BsaJI CCNNGG 1 cut(s) 31
BsaWI WCCGGW 1 cut(s) 308
Bsc4I CCNNNNNNNGG 4 cut(s) 21, 49, 213, 653
Bse118I RCCGGY 1 cut(s) 198
Bse1I ACTGG 5 cut(s) 199, 340, 396, 672, 786
BseBI CCWGG 1 cut(s) 1034
BseDI CCNNGG 1 cut(s) 31
BseGI GGATG 2 cut(s) 115, 976
BseLI CCNNNNNNNGG 4 cut(s) 21, 49, 213, 653
BseMII CTCAG 1 cut(s) 554
BseNI ACTGG 5 cut(s) 199, 340, 396, 672, 786
BseRI GAGGAG 2 cut(s) 17, 569
BseXI GCAGC 2 cut(s) 408, 869
BseYI CCCAGC 1 cut(s) 844
BsgI GTGCAG 1 cut(s) 68
BshFI GGCC 3 cut(s) 14, 198, 642
BsiHKAI GWGCWC 1 cut(s) 622
BsiSI CCGG 3 cut(s) 199, 309, 330
BslI CCNNNNNNNGG 4 cut(s) 21, 49, 213, 653
BsmAI GTCTC 2 cut(s) 90, 829
BsnI GGCC 3 cut(s) 14, 198, 642
Bsp119I TTCGAA 1 cut(s) 838
Bsp1286I GDGCHC 1 cut(s) 622
Bsp143I GATC 5 cut(s) 249, 277, 536, 946, 1012
BspACI CCGC 2 cut(s) 639, 885
BspANI GGCC 3 cut(s) 14, 198, 642
BspCNI CTCAG 1 cut(s) 553
BspLI GGNNCC 2 cut(s) 26, 279
BspMI ACCTGC 2 cut(s) 40, 274
BspPI GGATC 3 cut(s) 272, 285, 941
BspT104I TTCGAA 1 cut(s) 838
BsrFI RCCGGY 1 cut(s) 198
BsrI ACTGG 5 cut(s) 199, 340, 396, 672, 786
BssAI RCCGGY 1 cut(s) 198
BssECI CCNNGG 1 cut(s) 31
BssMI GATC 5 cut(s) 249, 277, 536, 946, 1012
BssT1I CCWWGG 1 cut(s) 31
Bst2UI CCWGG 1 cut(s) 1034
Bst4CI ACNGT 4 cut(s) 106, 139, 167, 212
BstBI TTCGAA 1 cut(s) 838
BstC8I GCNNGC 3 cut(s) 219, 662, 880
BstDEI CTNAG 1 cut(s) 540
BstENI CCTNNNNNAGG 1 cut(s) 47
BstF5I GGATG 2 cut(s) 115, 976
BstKTI GATC 5 cut(s) 252, 280, 539, 949, 1015
BstMAI GTCTC 2 cut(s) 90, 829
BstMBI GATC 5 cut(s) 249, 277, 536, 946, 1012
BstMWI GCNNNNNNNGC 3 cut(s) 435, 610, 1007
BstNI CCWGG 1 cut(s) 1034
BstNSI RCATGY 1 cut(s) 942
BstSCI CCNGG 1 cut(s) 1032
BstSFI CTRYAG 1 cut(s) 286
BstV1I GCAGC 2 cut(s) 408, 869
BstX2I RGATCY 2 cut(s) 277, 946
BstYI RGATCY 2 cut(s) 277, 946
BsuRI GGCC 3 cut(s) 14, 198, 642
BtsCI GGATG 2 cut(s) 115, 976
BtsIMutI CAGTG 1 cut(s) 665
BveI ACCTGC 2 cut(s) 40, 274
Cac8I GCNNGC 3 cut(s) 219, 662, 880
Cfr10I RCCGGY 1 cut(s) 198
CseI GACGC 1 cut(s) 435
CsiI ACCWGGT 1 cut(s) 1032
Csp6I GTAC 3 cut(s) 397, 559, 715
CviAII CATG 2 cut(s) 939, 1016
CviQI GTAC 3 cut(s) 397, 559, 715
DdeI CTNAG 1 cut(s) 540
DpnI GATC 5 cut(s) 251, 279, 538, 948, 1014
DpnII GATC 5 cut(s) 249, 277, 536, 946, 1012
DraI TTTAAA 1 cut(s) 1024
EaeI YGGCCR 1 cut(s) 196
Ecl136II GAGCTC 1 cut(s) 620
Eco130I CCWWGG 1 cut(s) 31
Eco24I GRGCYC 1 cut(s) 622
Eco32I GATATC 1 cut(s) 132
Eco53kI GAGCTC 1 cut(s) 620
Eco57I CTGAAG 1 cut(s) 258
EcoICRI GAGCTC 1 cut(s) 620
EcoNI CCTNNNNNAGG 1 cut(s) 47
EcoRII CCWGG 1 cut(s) 1032
EcoRV GATATC 1 cut(s) 132
EcoT14I CCWWGG 1 cut(s) 31
EcoT38I GRGCYC 1 cut(s) 622
ErhI CCWWGG 1 cut(s) 31
FaeI CATG 2 cut(s) 942, 1019
FalI AAGNNNNNCTT 2 cut(s) 137, 169
FatI CATG 2 cut(s) 938, 1015
FbaI TGATCA 1 cut(s) 249
Fnu4HI GCNGC 4 cut(s) 422, 640, 883, 886
FokI GGATG 2 cut(s) 122, 983
FriOI GRGCYC 1 cut(s) 622
Fsp4HI GCNGC 4 cut(s) 422, 640, 883, 886
FspBI CTAG 6 cut(s) 32, 44, 434, 506, 575, 729
GluI GCNGC 4 cut(s) 422, 640, 883, 886
GsaI CCCAGC 1 cut(s) 848
GsuI CTGGAG 2 cut(s) 413, 689
HaeIII GGCC 3 cut(s) 14, 198, 642
HapII CCGG 3 cut(s) 199, 309, 330
HgaI GACGC 1 cut(s) 435
Hin1II CATG 2 cut(s) 942, 1019
HinfI GANTC 1 cut(s) 98
HpaII CCGG 3 cut(s) 199, 309, 330
Hpy166II GTNNAC 1 cut(s) 936
Hpy188I TCNGA 7 cut(s) 238, 298, 358, 599, 624, 808, 946
Hpy188III TCNNGA 3 cut(s) 506, 772, 901
Hpy8I GTNNAC 1 cut(s) 936
Hpy99I CGWCG 1 cut(s) 429
HpyAV CCTTC 4 cut(s) 233, 653, 769, 985
HpyCH4III ACNGT 4 cut(s) 106, 139, 167, 212
HpyCH4V TGCA 6 cut(s) 49, 113, 221, 269, 751, 1010
HpyF10VI GCNNNNNNNGC 3 cut(s) 435, 610, 1007
HpyF3I CTNAG 1 cut(s) 540
Hsp92II CATG 2 cut(s) 942, 1019
Ksp22I TGATCA 1 cut(s) 249
Kzo9I GATC 5 cut(s) 249, 277, 536, 946, 1012
LmnI GCTCC 2 cut(s) 30, 617
Lsp1109I GCAGC 2 cut(s) 408, 869
LweI GCATC 2 cut(s) 100, 961
MabI ACCWGGT 1 cut(s) 1032
MaeI CTAG 6 cut(s) 32, 44, 434, 506, 575, 729
MaeIII GTNAC 1 cut(s) 167
MalI GATC 5 cut(s) 251, 279, 538, 948, 1014
MboI GATC 5 cut(s) 249, 277, 536, 946, 1012
MboII GAAGA 2 cut(s) 427, 637
MflI RGATCY 2 cut(s) 277, 946
MhlI GDGCHC 1 cut(s) 622
MluCI AATT 5 cut(s) 172, 579, 589, 788, 1040
MlyI GAGTC 1 cut(s) 92
MmeI TCCRAC 1 cut(s) 231
MnlI CCTC 7 cut(s) 38, 176, 334, 387, 448, 547, 657
MseI TTAA 3 cut(s) 9, 786, 1023
MslI CAYNNNNRTG 1 cut(s) 893
MspI CCGG 3 cut(s) 199, 309, 330
MspR9I CCNGG 1 cut(s) 1034
MvaI CCWGG 1 cut(s) 1034
MwoI GCNNNNNNNGC 3 cut(s) 435, 610, 1007
NdeII GATC 5 cut(s) 249, 277, 536, 946, 1012
NlaIII CATG 2 cut(s) 942, 1019
NlaIV GGNNCC 2 cut(s) 26, 279
NmuCI GTSAC 1 cut(s) 167
NspI RCATGY 1 cut(s) 942
NspV TTCGAA 1 cut(s) 838
PciI ACATGT 1 cut(s) 938
PflMI CCANNNNNTGG 1 cut(s) 21
PkrI GCNGC 4 cut(s) 423, 641, 884, 887
PleI GAGTC 1 cut(s) 92
PpsI GAGTC 1 cut(s) 92
PscI ACATGT 1 cut(s) 938
PsiI TTATAA 1 cut(s) 635
Psp124BI GAGCTC 1 cut(s) 622
Psp6I CCWGG 1 cut(s) 1032
PspFI CCCAGC 1 cut(s) 844
PspGI CCWGG 1 cut(s) 1032
PspN4I GGNNCC 2 cut(s) 26, 279
PsuI RGATCY 2 cut(s) 277, 946
RsaI GTAC 3 cut(s) 398, 560, 716
RsaNI GTAC 3 cut(s) 397, 559, 715
RseI CAYNNNNRTG 1 cut(s) 893
SacI GAGCTC 1 cut(s) 622
SaqAI TTAA 3 cut(s) 9, 786, 1023
SatI GCNGC 4 cut(s) 422, 640, 883, 886
Sau3AI GATC 5 cut(s) 249, 277, 536, 946, 1012
ScaI AGTACT 1 cut(s) 398
SchI GAGTC 1 cut(s) 92
ScrFI CCNGG 1 cut(s) 1034
SduI GDGCHC 1 cut(s) 622
SexAI ACCWGGT 1 cut(s) 1032
SfaNI GCATC 2 cut(s) 100, 961
SfcI CTRYAG 1 cut(s) 286
SfuI TTCGAA 1 cut(s) 838
SmiMI CAYNNNNRTG 1 cut(s) 893
SmlI CTYRAG 1 cut(s) 770
SmoI CTYRAG 1 cut(s) 770
Sse9I AATT 5 cut(s) 172, 579, 589, 788, 1040
SsiI CCGC 2 cut(s) 639, 885
SspMI CTAG 6 cut(s) 32, 44, 434, 506, 575, 729
SstI GAGCTC 1 cut(s) 622
StyD4I CCNGG 1 cut(s) 1032
StyI CCWWGG 1 cut(s) 31
TaaI ACNGT 4 cut(s) 106, 139, 167, 212
TaqI TCGA 3 cut(s) 134, 838, 900
TasI AATT 5 cut(s) 172, 579, 589, 788, 1040
TatI WGTACW 2 cut(s) 396, 714
TauI GCSGC 2 cut(s) 642, 888
Tru1I TTAA 3 cut(s) 9, 786, 1023
Tru9I TTAA 3 cut(s) 9, 786, 1023
TscAI CASTG 1 cut(s) 672
TseFI GTSAC 1 cut(s) 167
TseI GCWGC 2 cut(s) 421, 882
Tsp45I GTSAC 1 cut(s) 167
TspDTI ATGAA 1 cut(s) 583
TspGWI ACGGA 1 cut(s) 239
TspRI CASTG 1 cut(s) 672
Van91I CCANNNNNTGG 1 cut(s) 21
XagI CCTNNNNNAGG 1 cut(s) 47
XapI RAATTY 2 cut(s) 589, 788
XbaI TCTAGA 1 cut(s) 505
XceI RCATGY 1 cut(s) 942
XmaJI CCTAGG 1 cut(s) 31
XspI CTAG 6 cut(s) 32, 44, 434, 506, 575, 729
ZrmI AGTACT 1 cut(s) 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.