Rh5CG314800

GDSL esterase lipase At4g26790-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0011664
Physical Location & Seq
Reverse (-)
34804322 .. 34807120
2799 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG314800.1

Sequence Viewer

Length: 789 bp
ATGCTTTTTGTATTTATTCAAATAAAAGTACAGTTTAAATACAATAAACTAAAATCAAGAGTATACAGGATTGTCTTATTTACAGCCATCTTACTGGACTTGCAGTCTGTGATACCTCTTTGGAAGGAATTGGAGTACTACAAAGAATACCAGCAGGACCTCAGAATCTATCTAGGAAATGATGAAGCAAACGAGATTCTAGGAGAAGCACTTTACTTAATGAGTATAGGAACTAATGATTTCCTAGAAAACTACTATCTATTATCAAACAGATCATCGGAGTATTCCACAGAGGAGTACCAGAATTTTCTAGCAAAAATTGCTGGAAATTTTATCACAGAGCTCTTCCAGCTTGGAGCCCGAAAGATATCTCTAGGCGGCCTTCCTCCAATGGGTTGTCTGCCATTGGAGAGAACTAGAAATCTTCTTTTGGGGAGTGATTGTGTTGAGACGTACAATGATGTAGCTAGGAGTTTCAATAACAAGTTGCAGGAATTAGTTGACAGACTGAACGAGGAACTAGTTGGAATCCAGCTTGTGCTTGCAAACCCATACTATATTCTCTCAGATATCATACAAAACCCAGAATCCTTTGGATTTGAAGAAGCAGCAACAGCCTGTTGTGGTTCAGGATTGTTTGAGATGGGTTACTTGTGTACAAAGATAAACCCATTTACGTGTTCAGATGCAAATCAATATGTATTTTGGGATGCCTTTCACCCCACAGAGAGAACAAATGGTATTGTTGCAGATCATGTATTTAAAACTTGTCTAGCTCAATTTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

262

Amino Acids

30.1

Weight (kDa)

4.74

Isoelectric Point (pI)

32.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 65 - 253 2.1e-22 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011664)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26790 AT4G26790
fragaria_vesca FvH4_3g23490 FvH4_3g23490 FvH4_3g23490
malus_domestica MD11G1210200.v1.1
prunus_persica Prupe.4G222900_v2.0.a1
pyrus_communis pycom11g18580
rosa_chinensis RchiOBHm_Chr5g0041341
rosa_laevigata RLG00000034054
rosa_multiflora Rmu_sc0000594.1_g000072
rosa_roxburghii Rroxscaffold_1G00039290
rosa_rugosa Rorug05G0191600 Rorug05G0191700
rosa_samantha Rh5AG277700 Rh5BG282400 Rh5CG314800 Rh5DG291200
rosa_wichuraiana Rw5G026140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 63
AciI CCGC 1 cut(s) 378
AcsI RAATTY 2 cut(s) 304, 328
AfaI GTAC 5 cut(s) 30, 137, 299, 455, 658
AfiI CCNNNNNNNGG 1 cut(s) 392
AflIII ACRYGT 1 cut(s) 677
AgsI TTSAA 3 cut(s) 20, 478, 602
AhdI GACNNNNNGTC 1 cut(s) 103
AhlI ACTAGT 1 cut(s) 520
AluBI AGCT 5 cut(s) 343, 352, 467, 535, 776
AluI AGCT 5 cut(s) 343, 352, 467, 535, 776
Alw21I GWGCWC 1 cut(s) 345
Alw26I GTCTC 1 cut(s) 443
AoxI GGCC 1 cut(s) 379
ApeKI GCWGC 1 cut(s) 608
ApoI RAATTY 2 cut(s) 304, 328
AspS9I GGNCC 1 cut(s) 157
AsuHPI GGTGA 1 cut(s) 710
AvaII GGWCC 1 cut(s) 157
BanII GRGCYC 2 cut(s) 345, 361
Bbv12I GWGCWC 1 cut(s) 345
BbvI GCAGC 1 cut(s) 620
BccI CCATC 2 cut(s) 95, 637
BcoDI GTCTC 1 cut(s) 443
BcuI ACTAGT 1 cut(s) 520
BfaI CTAG 9 cut(s) 173, 200, 245, 311, 374, 417, 468, 521, 773
BisI GCNGC 2 cut(s) 379, 609
BlsI GCNGC 2 cut(s) 380, 610
BmcAI AGTACT 1 cut(s) 137
Bme18I GGWCC 1 cut(s) 157
BmeRI GACNNNNNGTC 1 cut(s) 103
BmgT120I GGNCC 1 cut(s) 157
BmiI GGNNCC 1 cut(s) 358
BmsI GCATC 2 cut(s) 676, 700
BsaAI YACGTR 1 cut(s) 678
BsaBI GATNNNNATC 1 cut(s) 690
BsaXI ACNNNNNCTCC 2 cut(s) 427, 457
Bsc4I CCNNNNNNNGG 1 cut(s) 392
Bse1I ACTGG 1 cut(s) 99
Bse8I GATNNNNATC 1 cut(s) 690
BseGI GGATG 1 cut(s) 715
BseJI GATNNNNATC 1 cut(s) 690
BseLI CCNNNNNNNGG 1 cut(s) 392
BseMII CTCAG 2 cut(s) 175, 579
BseNI ACTGG 1 cut(s) 99
BseRI GAGGAG 1 cut(s) 308
BseXI GCAGC 1 cut(s) 620
BshFI GGCC 1 cut(s) 381
BsiHKAI GWGCWC 1 cut(s) 345
BslI CCNNNNNNNGG 1 cut(s) 392
BsmAI GTCTC 1 cut(s) 443
BsmBI CGTCTC 1 cut(s) 443
BsnI GGCC 1 cut(s) 381
Bsp1286I GDGCHC 2 cut(s) 345, 361
Bsp1407I TGTACA 1 cut(s) 656
Bsp143I GATC 2 cut(s) 272, 751
BspACI CCGC 1 cut(s) 378
BspANI GGCC 1 cut(s) 381
BspCNI CTCAG 2 cut(s) 174, 578
BspLI GGNNCC 1 cut(s) 358
BspQI GCTCTTC 1 cut(s) 350
BsrGI TGTACA 1 cut(s) 656
BsrI ACTGG 1 cut(s) 99
BssMI GATC 2 cut(s) 272, 751
BssNAI GTATAC 1 cut(s) 64
Bst1107I GTATAC 1 cut(s) 64
Bst4CI ACNGT 1 cut(s) 33
Bst6I CTCTTC 1 cut(s) 350
BstAPI GCANNNNNTGC 1 cut(s) 320
BstAUI TGTACA 1 cut(s) 656
BstBAI YACGTR 1 cut(s) 678
BstC8I GCNNGC 1 cut(s) 543
BstDEI CTNAG 2 cut(s) 161, 565
BstF5I GGATG 1 cut(s) 715
BstKTI GATC 2 cut(s) 275, 754
BstMAI GTCTC 1 cut(s) 443
BstMBI GATC 2 cut(s) 272, 751
BstMWI GCNNNNNNNGC 3 cut(s) 320, 349, 614
BstV1I GCAGC 1 cut(s) 620
BstXI CCANNNNNNTGG 1 cut(s) 94
BstZ17I GTATAC 1 cut(s) 64
BsuRI GGCC 1 cut(s) 381
BtsCI GGATG 1 cut(s) 715
Cac8I GCNNGC 1 cut(s) 543
Cfr13I GGNCC 1 cut(s) 157
Csp6I GTAC 5 cut(s) 29, 136, 298, 454, 657
CviAII CATG 1 cut(s) 755
CviJI RGCY 9 cut(s) 86, 343, 352, 359, 381, 467, 535, 617, 776
CviKI_1 RGCY 9 cut(s) 86, 343, 352, 359, 381, 467, 535, 617, 776
CviQI GTAC 5 cut(s) 29, 136, 298, 454, 657
DdeI CTNAG 2 cut(s) 161, 565
DpnI GATC 2 cut(s) 274, 753
DpnII GATC 2 cut(s) 272, 751
DraI TTTAAA 2 cut(s) 37, 763
DriI GACNNNNNGTC 1 cut(s) 103
Eam1104I CTCTTC 1 cut(s) 350
Eam1105I GACNNNNNGTC 1 cut(s) 103
EarI CTCTTC 1 cut(s) 350
Ecl136II GAGCTC 1 cut(s) 343
Eco24I GRGCYC 2 cut(s) 345, 361
Eco32I GATATC 2 cut(s) 369, 571
Eco47I GGWCC 1 cut(s) 157
Eco53kI GAGCTC 1 cut(s) 343
EcoICRI GAGCTC 1 cut(s) 343
EcoO109I RGGNCCY 1 cut(s) 157
EcoRV GATATC 2 cut(s) 369, 571
EcoT38I GRGCYC 2 cut(s) 345, 361
Esp3I CGTCTC 1 cut(s) 443
FaeI CATG 1 cut(s) 758
FaiI YATR 8 cut(s) 64, 227, 553, 558, 575, 699, 756, 787
FatI CATG 1 cut(s) 754
FblI GTMKAC 1 cut(s) 63
Fnu4HI GCNGC 2 cut(s) 379, 609
FokI GGATG 1 cut(s) 722
FriOI GRGCYC 2 cut(s) 345, 361
Fsp4HI GCNGC 2 cut(s) 379, 609
FspBI CTAG 9 cut(s) 173, 200, 245, 311, 374, 417, 468, 521, 773
GluI GCNGC 2 cut(s) 379, 609
HaeIII GGCC 1 cut(s) 381
Hin1II CATG 1 cut(s) 758
HincII GTYRAC 1 cut(s) 502
HindII GTYRAC 1 cut(s) 502
HinfI GANTC 4 cut(s) 165, 196, 528, 587
HphI GGTGA 1 cut(s) 710
Hpy166II GTNNAC 3 cut(s) 64, 502, 657
Hpy188I TCNGA 4 cut(s) 164, 280, 568, 685
Hpy188III TCNNGA 2 cut(s) 57, 630
Hpy8I GTNNAC 3 cut(s) 64, 502, 657
HpyAV CCTTC 2 cut(s) 118, 392
HpyCH4III ACNGT 1 cut(s) 33
HpyCH4IV ACGT 2 cut(s) 452, 677
HpyCH4V TGCA 5 cut(s) 103, 490, 545, 689, 749
HpyF10VI GCNNNNNNNGC 3 cut(s) 320, 349, 614
HpyF3I CTNAG 2 cut(s) 161, 565
HpySE526I ACGT 2 cut(s) 452, 677
Hsp92II CATG 1 cut(s) 758
Kzo9I GATC 2 cut(s) 272, 751
LguI GCTCTTC 1 cut(s) 350
LmnI GCTCC 1 cut(s) 356
Lsp1109I GCAGC 1 cut(s) 620
LweI GCATC 2 cut(s) 676, 700
MaeI CTAG 9 cut(s) 173, 200, 245, 311, 374, 417, 468, 521, 773
MaeII ACGT 2 cut(s) 452, 677
MaeIII GTNAC 1 cut(s) 647
MalI GATC 2 cut(s) 274, 753
MboI GATC 2 cut(s) 272, 751
MboII GAAGA 3 cut(s) 337, 416, 614
MhlI GDGCHC 2 cut(s) 345, 361
MluCI AATT 6 cut(s) 128, 304, 318, 328, 494, 779
MmeI TCCRAC 1 cut(s) 505
MnlI CCTC 5 cut(s) 126, 170, 286, 396, 508
MseI TTAA 3 cut(s) 36, 218, 762
MslI CAYNNNNRTG 1 cut(s) 676
MwoI GCNNNNNNNGC 3 cut(s) 320, 349, 614
NdeII GATC 2 cut(s) 272, 751
NlaIII CATG 1 cut(s) 758
NlaIV GGNNCC 1 cut(s) 358
PciSI GCTCTTC 1 cut(s) 350
PfeI GAWTC 4 cut(s) 165, 196, 528, 587
PkrI GCNGC 2 cut(s) 380, 610
Ppu21I YACGTR 1 cut(s) 678
PpuMI RGGWCCY 1 cut(s) 157
Psp124BI GAGCTC 1 cut(s) 345
Psp5II RGGWCCY 1 cut(s) 157
PspN4I GGNNCC 1 cut(s) 358
PspPI GGNCC 1 cut(s) 157
PspPPI RGGWCCY 1 cut(s) 157
RsaI GTAC 5 cut(s) 30, 137, 299, 455, 658
RsaNI GTAC 5 cut(s) 29, 136, 298, 454, 657
RseI CAYNNNNRTG 1 cut(s) 676
SacI GAGCTC 1 cut(s) 345
SapI GCTCTTC 1 cut(s) 350
SaqAI TTAA 3 cut(s) 36, 218, 762
SatI GCNGC 2 cut(s) 379, 609
Sau3AI GATC 2 cut(s) 272, 751
Sau96I GGNCC 1 cut(s) 157
ScaI AGTACT 1 cut(s) 137
SduI GDGCHC 2 cut(s) 345, 361
SetI ASST 9 cut(s) 118, 162, 345, 354, 455, 469, 537, 680, 778
SfaNI GCATC 2 cut(s) 676, 700
SinI GGWCC 1 cut(s) 157
SmiMI CAYNNNNRTG 1 cut(s) 676
SpeI ACTAGT 1 cut(s) 520
Sse9I AATT 6 cut(s) 128, 304, 318, 328, 494, 779
SsiI CCGC 1 cut(s) 378
SspMI CTAG 9 cut(s) 173, 200, 245, 311, 374, 417, 468, 521, 773
SstI GAGCTC 1 cut(s) 345
TaaI ACNGT 1 cut(s) 33
TaiI ACGT 2 cut(s) 455, 680
TasI AATT 6 cut(s) 128, 304, 318, 328, 494, 779
TatI WGTACW 3 cut(s) 28, 135, 656
TauI GCSGC 1 cut(s) 381
TfiI GAWTC 4 cut(s) 165, 196, 528, 587
Tru1I TTAA 3 cut(s) 36, 218, 762
Tru9I TTAA 3 cut(s) 36, 218, 762
TseI GCWGC 1 cut(s) 608
TspDTI ATGAA 1 cut(s) 198
VpaK11BI GGWCC 1 cut(s) 157
XapI RAATTY 2 cut(s) 304, 328
XmiI GTMKAC 1 cut(s) 63
XspI CTAG 9 cut(s) 173, 200, 245, 311, 374, 417, 468, 521, 773
ZrmI AGTACT 1 cut(s) 137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.