Rroxscaffold_1G00039290

GDSL esterase lipase At4g26790-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0011664
Physical Location & Seq
Forward (+)
56884407 .. 56885896
1490 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00039290.1

Sequence Viewer

Length: 1095 bp
ATGGCATTTAAAGCAATTTATTGGTTTCTCCTAACCCACTTCCTCCTGCAAATTTCTAGGGTGTGTGCAAGAGTTCCGGTAATCATTGTGTTTGGAGACTCTTCGGTTGATGCAGGAAACAATAACCAGGTATCAACAATCCTAAAGAGTGACTTTCCACCCTACGGTCGTGATTTCTACGGTGGACAGCCTACAGGGAGATTTTCCAATGGGAGGATTCCACCAGACTTCATTTCTGAAGCTTTTGGGATCAAGCCATTGATACCTGCATTTTTGGATCCATTTTATAACATTAAGGATTTCGCTACTGGAGTTTGCTTTGCCTCCGCAGGAACTGGTTATGATAATGCCACTTCTGATGTCCTAGTAAGTCACACTACCACTGAATCTACTATCTTGCTGGACTTGCAGTCTGTGATACCTCTTTGGAAGGAATTGGAGTACTACAAAGAATACCAGCAGGACCTCAGAATCTATCTTGGAAATGATGAAGCAAATGAAATTCTAGGAGAAGCACTTTACTTGATGAGTATAGGAACTAATGATTTCCTAGAAAACTACTATCTATTTTCAAACAGATCATCGGAGTATTCCATAGAGGAGTACCAGAATTTTCTAGCAAAACTTGCTGGAAATTTCATCACAGAGCTCTTCCAGCTTGGAGCTCGAAAGATATCTCTAGGCGGCCTTCCTCCAATGGGTTGTCTGCCATTGGAGAGAACTAGAAATCTTCTTTCGGGGAGTGATTGTGTTGAGACGTACAATGATGTGGCTAGGAGTTTCAATAACAAGTTGGAGGAATTAGTTGACAAATTCAACGAGGAACTAGTTGGAATCCAGCTTGTGCTTTCAAACCCATACTATATTCTCTCAGATATCATACGAAACCCTAAATCCTTTGGATTTGAAGAAGCAGCAACAGCATGTTGTGGTACAGGATTGTTTGAGATGGGTTACATGTGTACAAAGATAAACCCATTTACGTGTTCAGATGCAAAGCAATATGTATTTTGGGATGCCTTCCACCCCACAGAGAGAACAAATGGTATTGTTGCAGATCATTTATTTAAAACTTGTCTAGCTCAATTTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

364

Amino Acids

40.94

Weight (kDa)

4.69

Isoelectric Point (pI)

34.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 28 - 355 1.5e-31 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011664)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26790 AT4G26790
fragaria_vesca FvH4_3g23490 FvH4_3g23490 FvH4_3g23490
malus_domestica MD11G1210200.v1.1
prunus_persica Prupe.4G222900_v2.0.a1
pyrus_communis pycom11g18580
rosa_chinensis RchiOBHm_Chr5g0041341
rosa_laevigata RLG00000034054
rosa_multiflora Rmu_sc0000594.1_g000072
rosa_roxburghii Rroxscaffold_1G00039290
rosa_rugosa Rorug05G0191600 Rorug05G0191700
rosa_samantha Rh5AG277700 Rh5BG282400 Rh5CG314800 Rh5DG291200
rosa_wichuraiana Rw5G026140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 288
Acc36I ACCTGC 1 cut(s) 274
AciI CCGC 2 cut(s) 327, 684
AclWI GGATC 3 cut(s) 257, 272, 285
AcsI RAATTY 5 cut(s) 51, 501, 610, 634, 812
AcuI CTGAAG 1 cut(s) 258
AfaI GTAC 5 cut(s) 443, 605, 761, 934, 964
AfiI CCNNNNNNNGG 3 cut(s) 164, 213, 698
AflIII ACRYGT 2 cut(s) 957, 983
AgsI TTSAA 5 cut(s) 573, 784, 817, 852, 908
AhdI GACNNNNNGTC 1 cut(s) 409
AhlI ACTAGT 1 cut(s) 826
AjnI CCWGG 1 cut(s) 126
AluBI AGCT 6 cut(s) 242, 649, 658, 665, 841, 1082
AluI AGCT 6 cut(s) 242, 649, 658, 665, 841, 1082
Alw21I GWGCWC 2 cut(s) 651, 667
Alw26I GTCTC 2 cut(s) 90, 749
AlwI GGATC 3 cut(s) 257, 272, 285
AlwNI CAGNNNCTG 1 cut(s) 335
AoxI GGCC 1 cut(s) 685
ApeKI GCWGC 1 cut(s) 914
ApoI RAATTY 5 cut(s) 51, 501, 610, 634, 812
AspS9I GGNCC 1 cut(s) 463
AvaII GGWCC 1 cut(s) 463
BamHI GGATCC 1 cut(s) 277
BanII GRGCYC 2 cut(s) 651, 667
Bbv12I GWGCWC 2 cut(s) 651, 667
BbvI GCAGC 1 cut(s) 926
BccI CCATC 1 cut(s) 943
BciT130I CCWGG 1 cut(s) 128
BcoDI GTCTC 2 cut(s) 90, 749
BcuI ACTAGT 1 cut(s) 826
BfmI CTRYAG 1 cut(s) 192
BfuAI ACCTGC 1 cut(s) 274
BisI GCNGC 2 cut(s) 685, 915
BlsI GCNGC 2 cut(s) 686, 916
BmcAI AGTACT 1 cut(s) 443
Bme1390I CCNGG 1 cut(s) 128
Bme18I GGWCC 1 cut(s) 463
BmeRI GACNNNNNGTC 1 cut(s) 409
BmgT120I GGNCC 1 cut(s) 463
BmiI GGNNCC 1 cut(s) 279
BmrFI CCNGG 1 cut(s) 128
BmsI GCATC 3 cut(s) 100, 982, 1006
BpmI CTGGAG 1 cut(s) 330
BsaAI YACGTR 1 cut(s) 984
BsaWI WCCGGW 1 cut(s) 76
BsaXI ACNNNNNCTCC 2 cut(s) 733, 763
Bsc4I CCNNNNNNNGG 3 cut(s) 164, 213, 698
Bse1I ACTGG 2 cut(s) 313, 340
BseBI CCWGG 1 cut(s) 128
BseGI GGATG 1 cut(s) 1021
BseLI CCNNNNNNNGG 3 cut(s) 164, 213, 698
BseMII CTCAG 2 cut(s) 481, 885
BseNI ACTGG 2 cut(s) 313, 340
BseRI GAGGAG 1 cut(s) 614
BseXI GCAGC 1 cut(s) 926
Bsh1285I CGRYCG 1 cut(s) 169
BshFI GGCC 1 cut(s) 687
BsiEI CGRYCG 1 cut(s) 169
BsiHKAI GWGCWC 2 cut(s) 651, 667
BsiSI CCGG 1 cut(s) 77
BslI CCNNNNNNNGG 3 cut(s) 164, 213, 698
BsmAI GTCTC 2 cut(s) 90, 749
BsmBI CGTCTC 1 cut(s) 749
BsnI GGCC 1 cut(s) 687
Bsp1286I GDGCHC 2 cut(s) 651, 667
Bsp1407I TGTACA 1 cut(s) 962
Bsp143I GATC 4 cut(s) 249, 277, 578, 1057
BspACI CCGC 2 cut(s) 327, 684
BspANI GGCC 1 cut(s) 687
BspCNI CTCAG 2 cut(s) 480, 884
BspLI GGNNCC 1 cut(s) 279
BspMI ACCTGC 1 cut(s) 274
BspPI GGATC 3 cut(s) 257, 272, 285
BspQI GCTCTTC 1 cut(s) 656
BsrGI TGTACA 1 cut(s) 962
BsrI ACTGG 2 cut(s) 313, 340
BssMI GATC 4 cut(s) 249, 277, 578, 1057
Bst2UI CCWGG 1 cut(s) 128
Bst4CI ACNGT 2 cut(s) 167, 182
Bst6I CTCTTC 2 cut(s) 106, 656
BstAPI GCANNNNNTGC 1 cut(s) 626
BstAUI TGTACA 1 cut(s) 962
BstBAI YACGTR 1 cut(s) 984
BstDEI CTNAG 2 cut(s) 467, 871
BstF5I GGATG 1 cut(s) 1021
BstKTI GATC 4 cut(s) 252, 280, 581, 1060
BstMAI GTCTC 2 cut(s) 90, 749
BstMBI GATC 4 cut(s) 249, 277, 578, 1057
BstMCI CGRYCG 1 cut(s) 169
BstMWI GCNNNNNNNGC 5 cut(s) 11, 406, 626, 655, 920
BstNI CCWGG 1 cut(s) 128
BstNSI RCATGY 2 cut(s) 927, 961
BstSCI CCNGG 1 cut(s) 126
BstSFI CTRYAG 1 cut(s) 192
BstV1I GCAGC 1 cut(s) 926
BstX2I RGATCY 1 cut(s) 277
BstYI RGATCY 1 cut(s) 277
BsuRI GGCC 1 cut(s) 687
BtsCI GGATG 1 cut(s) 1021
BtsIMutI CAGTG 1 cut(s) 381
BveI ACCTGC 1 cut(s) 274
CaiI CAGNNNCTG 1 cut(s) 335
Cfr13I GGNCC 1 cut(s) 463
CsiI ACCWGGT 1 cut(s) 126
Csp6I GTAC 5 cut(s) 442, 604, 760, 933, 963
CviAII CATG 2 cut(s) 924, 958
CviQI GTAC 5 cut(s) 442, 604, 760, 933, 963
DdeI CTNAG 2 cut(s) 467, 871
DpnI GATC 4 cut(s) 251, 279, 580, 1059
DpnII GATC 4 cut(s) 249, 277, 578, 1057
DraI TTTAAA 2 cut(s) 10, 1069
DriI GACNNNNNGTC 1 cut(s) 409
Eam1104I CTCTTC 2 cut(s) 106, 656
Eam1105I GACNNNNNGTC 1 cut(s) 409
EarI CTCTTC 2 cut(s) 106, 656
Ecl136II GAGCTC 2 cut(s) 649, 665
Eco24I GRGCYC 2 cut(s) 651, 667
Eco32I GATATC 2 cut(s) 675, 877
Eco47I GGWCC 1 cut(s) 463
Eco53kI GAGCTC 2 cut(s) 649, 665
Eco57I CTGAAG 1 cut(s) 258
EcoICRI GAGCTC 2 cut(s) 649, 665
EcoO109I RGGNCCY 1 cut(s) 463
EcoRII CCWGG 1 cut(s) 126
EcoRV GATATC 2 cut(s) 675, 877
EcoT38I GRGCYC 2 cut(s) 651, 667
Esp3I CGTCTC 1 cut(s) 749
FaeI CATG 2 cut(s) 927, 961
FalI AAGNNNNNCTT 2 cut(s) 137, 169
FatI CATG 2 cut(s) 923, 957
Fnu4HI GCNGC 2 cut(s) 685, 915
FokI GGATG 1 cut(s) 1028
FriOI GRGCYC 2 cut(s) 651, 667
Fsp4HI GCNGC 2 cut(s) 685, 915
GluI GCNGC 2 cut(s) 685, 915
GsuI CTGGAG 1 cut(s) 330
HaeIII GGCC 1 cut(s) 687
HapII CCGG 1 cut(s) 77
Hin1II CATG 2 cut(s) 927, 961
HincII GTYRAC 1 cut(s) 808
HindII GTYRAC 1 cut(s) 808
HindIII AAGCTT 1 cut(s) 240
HinfI GANTC 5 cut(s) 98, 217, 386, 471, 834
HpaII CCGG 1 cut(s) 77
Hpy166II GTNNAC 3 cut(s) 185, 808, 963
Hpy188I TCNGA 6 cut(s) 238, 358, 470, 586, 874, 991
Hpy188III TCNNGA 1 cut(s) 170
Hpy8I GTNNAC 3 cut(s) 185, 808, 963
HpyAV CCTTC 3 cut(s) 424, 698, 1030
HpyCH4III ACNGT 2 cut(s) 167, 182
HpyCH4IV ACGT 2 cut(s) 758, 983
HpyCH4V TGCA 7 cut(s) 49, 68, 113, 269, 409, 995, 1055
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 406, 626, 655, 920
HpyF3I CTNAG 2 cut(s) 467, 871
HpySE526I ACGT 2 cut(s) 758, 983
Hsp92II CATG 2 cut(s) 927, 961
Kzo9I GATC 4 cut(s) 249, 277, 578, 1057
LguI GCTCTTC 1 cut(s) 656
LmnI GCTCC 1 cut(s) 662
Lsp1109I GCAGC 1 cut(s) 926
LweI GCATC 3 cut(s) 100, 982, 1006
MabI ACCWGGT 1 cut(s) 126
MaeII ACGT 2 cut(s) 758, 983
MaeIII GTNAC 3 cut(s) 149, 371, 953
MalI GATC 4 cut(s) 251, 279, 580, 1059
MboI GATC 4 cut(s) 249, 277, 578, 1057
MboII GAAGA 4 cut(s) 93, 643, 722, 920
MflI RGATCY 1 cut(s) 277
MhlI GDGCHC 2 cut(s) 651, 667
MluCI AATT 9 cut(s) 15, 51, 434, 501, 610, 634, 800, 812, 1085
MlyI GAGTC 1 cut(s) 92
MmeI TCCRAC 2 cut(s) 774, 811
MnlI CCTC 9 cut(s) 53, 207, 334, 432, 476, 592, 702, 790, 814
MseI TTAA 3 cut(s) 9, 294, 1068
MslI CAYNNNNRTG 1 cut(s) 982
MspI CCGG 1 cut(s) 77
MspR9I CCNGG 1 cut(s) 128
MvaI CCWGG 1 cut(s) 128
MwoI GCNNNNNNNGC 5 cut(s) 11, 406, 626, 655, 920
NdeII GATC 4 cut(s) 249, 277, 578, 1057
NlaIII CATG 2 cut(s) 927, 961
NlaIV GGNNCC 1 cut(s) 279
NmuCI GTSAC 2 cut(s) 149, 371
NspI RCATGY 2 cut(s) 927, 961
PciI ACATGT 1 cut(s) 957
PciSI GCTCTTC 1 cut(s) 656
PfeI GAWTC 4 cut(s) 217, 386, 471, 834
PkrI GCNGC 2 cut(s) 686, 916
PleI GAGTC 1 cut(s) 92
PpsI GAGTC 1 cut(s) 92
Ppu21I YACGTR 1 cut(s) 984
PpuMI RGGWCCY 1 cut(s) 463
PscI ACATGT 1 cut(s) 957
PsiI TTATAA 1 cut(s) 288
Psp124BI GAGCTC 2 cut(s) 651, 667
Psp5II RGGWCCY 1 cut(s) 463
Psp6I CCWGG 1 cut(s) 126
PspGI CCWGG 1 cut(s) 126
PspN4I GGNNCC 1 cut(s) 279
PspPI GGNCC 1 cut(s) 463
PspPPI RGGWCCY 1 cut(s) 463
PstNI CAGNNNCTG 1 cut(s) 335
PsuI RGATCY 1 cut(s) 277
RsaI GTAC 5 cut(s) 443, 605, 761, 934, 964
RsaNI GTAC 5 cut(s) 442, 604, 760, 933, 963
RseI CAYNNNNRTG 1 cut(s) 982
SacI GAGCTC 2 cut(s) 651, 667
SapI GCTCTTC 1 cut(s) 656
SaqAI TTAA 3 cut(s) 9, 294, 1068
SatI GCNGC 2 cut(s) 685, 915
Sau3AI GATC 4 cut(s) 249, 277, 578, 1057
Sau96I GGNCC 1 cut(s) 463
ScaI AGTACT 1 cut(s) 443
SchI GAGTC 1 cut(s) 92
ScrFI CCNGG 1 cut(s) 128
SduI GDGCHC 2 cut(s) 651, 667
SexAI ACCWGGT 1 cut(s) 126
SfaNI GCATC 3 cut(s) 100, 982, 1006
SfcI CTRYAG 1 cut(s) 192
SinI GGWCC 1 cut(s) 463
SmiMI CAYNNNNRTG 1 cut(s) 982
SpeI ACTAGT 1 cut(s) 826
Sse9I AATT 9 cut(s) 15, 51, 434, 501, 610, 634, 800, 812, 1085
SsiI CCGC 2 cut(s) 327, 684
SstI GAGCTC 2 cut(s) 651, 667
StyD4I CCNGG 1 cut(s) 126
TaaI ACNGT 2 cut(s) 167, 182
TaiI ACGT 2 cut(s) 761, 986
TaqI TCGA 1 cut(s) 667
TasI AATT 9 cut(s) 15, 51, 434, 501, 610, 634, 800, 812, 1085
TatI WGTACW 2 cut(s) 441, 962
TauI GCSGC 1 cut(s) 687
TfiI GAWTC 4 cut(s) 217, 386, 471, 834
Tru1I TTAA 3 cut(s) 9, 294, 1068
Tru9I TTAA 3 cut(s) 9, 294, 1068
TscAI CASTG 1 cut(s) 388
TseFI GTSAC 2 cut(s) 149, 371
TseI GCWGC 1 cut(s) 914
Tsp45I GTSAC 2 cut(s) 149, 371
TspDTI ATGAA 4 cut(s) 220, 504, 513, 628
TspRI CASTG 1 cut(s) 388
VpaK11BI GGWCC 1 cut(s) 463
XapI RAATTY 5 cut(s) 51, 501, 610, 634, 812
XceI RCATGY 2 cut(s) 927, 961
ZrmI AGTACT 1 cut(s) 443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.