Rorug05G0191600

GDSL esterase lipase At4g26790-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0011664
Physical Location & Seq
Forward (+)
18625183 .. 18632909
7727 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0191600.1

Sequence Viewer

Length: 3093 bp
ATGCTCATATTAGTTGGAAGCATGCAGGGAACAATGGTTTCTTTGGCTTCCCTTGTTAGTTTGGGAAGTGTTGTTACGCTTGCGGGTTCATCCGAAAGGTCTGGCTCGCTTGTTAAGAAGGTTTCGTTGTCTAAGAGTAGTTTTAGGGGTAATAGGAGATGGCATTGTGTGAGATTGTCAGTTTGTAAATTTTCGGTCACAACAACTGATTTCGTTGCTGAGCATAGCAATGAGGTATCCCTTGATTCTAACTATAGAGGAAGTAGTAGTAATGATGCTTCTGTTGCCAATGCTGATTTTCTACTTAAGCCGTCCCCGAAGCCAGTATTGAAGCCCTCTGAGGGGTCCAATGCTGAACCGCCCCTTTTAAGCCTTAATGCTGCTGATTGGGACGCCTCAAAAACTAGTGGGGATTCGGATGTGGAAGAGGAAGATAAGAGTAATGTGATTGAGTCACTTGGAGAGGTTTTGGAGAAGGCTGAAAAGCTAGAAGCTCCAAAAGTGGGTGATTCGAGTACTAAGAAAGCCAGTAAGCCTGTAAATAGACCTGCACCATCTAATGCAACTACTACTTCAGGAAATGCTAGACCAGTAAACTCGACAGCTAGTACCAAGGCTAAAACTTTGAAGAGTGTGTGGCGTAAAGGAGACACAGTTGCAGCTGTGCAAAAGGTTGTAAAGGAATTACCTAAGGTTAATAATACAGTTCGGAGAGAAGAACCAAAAACAGGGGAAGGGGTGAAGGTAGAGTCGCCAACCCGTCCTCCTTTAAGACCTCATTCTCCACCTTTGAGACCCCAACCAACATTACAAGCAAAGCCTTCTACAGCTCCTCCACCCACGATAAAGAAACCTGTAGTTCTGAAGGATCTTGGGGCAGCTCCAAAGTCGGCAGTGATTGATGACACTGGTTCACCCCCAAAAACTAAAGAAAGGAAGCCAATTTTGATTGACAAATTTTCTACCAAGAAGCCAGGGGTCGATTCTGTGGTTTCTCAAGCAGTTTTAGCTCCTACAAAACCAGCCAAGGGCTCTCCGCCTGGCAGATTCAAAGATGGGTTCCGGAAGAAAAATGCTCAACCTGGAGGACTACGAAGGCGGAAGGCTAATGATGAGCTCACTGATGATGAGAGTTCGGAACTTAATGTCTCTAAAGCAGCAAGGAAAGGGAGGAAGTGGAGTAAAGCAAGCAGGAAGGCGGCAAGACTCCAGGCTGCCAAAGATGCAGCTCCTGTTAAAGTAGATATTCTAGAGGTTGATGACGATGGCATGTTAATAGATGAGTTGGCCTTCAATTTGGCTATTAATGAATCCGAAATTCTCGGATCTCTGTACTCCAGGGGAATTAAGCCTGATGGGGTTCAAACTCTGAGCAAAGATATGGTAAAGATGATTTGTAAAGAGTATGATGTGGAAGTCATAGATGTTGACCCAGTAAAGGTGGAAGAAGGGGCAAGAAAGAAAGAAATACTCGATGAAGATGACCTAGACAAACTAGAAGACAGGCCTCCTGTCCTCACTATAATGGGTCATGTGGATCATGGCAAGACAACTCTATTGGATTACATCCGGAAGAGCAAGGTTGCTGCCTCCGAGGCTGGTGGAATTACACAAGGAATTGGGGCATATAAGGTGCTCGTGCCAATTGATGGCAAGTTGCAATCATGTGTTTTCCTTGATACTCCTGGACATGAGGCATTTGGAGCAATGAGAGCTCGTGGAGCAAGGGTGACAGACATAGCTATCATTGTTGTGGCTGCTGATGATGGAATCCGCCCTCAAACAAAAGAGGCCATCGCTCATGCTAAAGCTGCTGGTGTGCCGATAGTAATAGCTATAAATAAGATAGACAAGGATGGAGCTAATCCAGAACGAGTCATGCAAGAGCTTTCTTCTATTGGTCTAATGCCGGAGGATTGGGGTGGTGACGTCCCAATGGTCCAGATTAGTGCTCTTAAAGGGAAGAATATAGATGACCTTTTGGAAACCGTAATGCTAGTTGCAGAGTTGCAAGAGTTAAAGGCCAATCCTGATAGAAGTGCAAAAGGCACGGTTATTGAGGCCGGTCTTGATAAATCCAGAGGACCTTTAGTTACACTTATCGTGCAAAATGGAACCCTTAGAAAGGGGGACATAGTAATTTGTGGAGAAGCCTTTGGAAAGGTACGGGCTTTATTTGATCATGGTGGAAATCGTGTCAATGAAGCCGGACCCTCTATACCAGTTCAGGTTATTGGATTAAATAATGTTCCTATTGCTGGTGATGAATTTGAGGTCGTGAGCTCCCTTGATATTGCACGTGAAAGGGCAGAGTCACGTGCAGACACATTGCGAAATGAACGCATATCAGCTAAGGCTGGCGATGGGAAGGTTACTCTTTCTTCTTTAGCTTCTGCAGTCTCGGCAGGAAAGCTGGCTGGACTTGACTTGCACCAGCTAAATATTATTCTGAAGGTTGATCTTCAGGGATCTATTGAGGCTGTCAGGCAAGCCCTGCAGGTCCTCCCTCAAGATAATGTCACCTTGAAGTTTCTCATGGAAACAACAGGTGATGTAAACACCAGCGATGTTGATCTTGCTGCGGCCAGCAAAGCCATAATTTTGGGATTCAATATCAAAGCTCCAGGTTCTGTTAAAAGCTATGCTGAAAACAAAGGTGTGGAGATTCGGCTATATAAAGTTATCTATGACCTTATTGATGATGTACGAAATGCAATGGAAGGACTCCTACAGCCTGTTGAGGAACAAGTAACAATTGGTTCTGCAGAAGTCCGAGCCATATTCAGCAGTGGCAGTGGACGTGTTGCTGGTTGTATGGTAAATGAAGGAAAAGTTGTTAAAGGCTGCGGCATTCAGGTCATTCGAAGGGGAAAAGTAGTACATGATGGTGTTCTTGATTCTTTAAAACGGGTTAAGGAAGTCGTGAAAGAGGTAAATGCTGGACTAGAGTGTGGAATTGGGGTGGAAGACTACGACGATTTTGAGGAGGGAGACATTCTCGAGGCCTTCAACACAGTCCAAAAGAAGCGGACACTTGAAGAGGCGTCTGCTTCCATGGCAGCTGCAGTGGAAGGAACAGGAGTCGAATACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

1030

Amino Acids

109.83

Weight (kDa)

7.15

Isoelectric Point (pI)

35.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 506 - 666 3.4e-34 Elongation factor Tu GTP binding domain
MMR_HSR1 PF01926 507 - 615 1.8e-08 50S ribosome-binding GTPase
EF-G_D2 PF22042 681 - 759 1e-25 Elongation factor G domain 2
IF-2 PF11987 809 - 903 1.7e-31 Translation-initiation factor 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011664)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26790 AT4G26790
fragaria_vesca FvH4_3g23490 FvH4_3g23490 FvH4_3g23490
malus_domestica MD11G1210200.v1.1
prunus_persica Prupe.4G222900_v2.0.a1
pyrus_communis pycom11g18580
rosa_chinensis RchiOBHm_Chr5g0041341
rosa_laevigata RLG00000034054
rosa_multiflora Rmu_sc0000594.1_g000072
rosa_roxburghii Rroxscaffold_1G00039290
rosa_rugosa Rorug05G0191600 Rorug05G0191700
rosa_samantha Rh5AG277700 Rh5BG282400 Rh5CG314800 Rh5DG291200
rosa_wichuraiana Rw5G026140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1932
Acc36I ACCTGC 2 cut(s) 556, 2488
AccB7I CCANNNNNTGG 1 cut(s) 1649
AccIII TCCGGA 2 cut(s) 1062, 1569
AciI CCGC 9 cut(s) 83, 359, 1037, 1099, 1199, 1774, 2582, 2847, 3028
AclWI GGATC 4 cut(s) 876, 1333, 1545, 2476
AcoI YGGCCR 1 cut(s) 2583
AcsI RAATTY 4 cut(s) 188, 956, 1317, 2267
AcuI CTGAAG 4 cut(s) 558, 884, 2447, 2471
AcvI CACGTG 2 cut(s) 2300, 2318
AcyI GRCGYC 3 cut(s) 393, 1929, 3044
AfaI GTAC 6 cut(s) 517, 610, 1334, 2166, 2706, 2880
AfiI CCNNNNNNNGG 9 cut(s) 341, 342, 503, 728, 1028, 1438, 1649, 2125, 2258
AflII CTTAAG 1 cut(s) 305
AflIII ACRYGT 1 cut(s) 2800
AgsI TTSAA 9 cut(s) 331, 628, 1051, 1294, 1364, 2527, 2611, 3010, 3038
AhlI ACTAGT 1 cut(s) 404
AjiI CACGTC 1 cut(s) 2801
AjnI CCWGG 7 cut(s) 973, 1039, 1081, 1209, 1337, 1684, 2623
Alw21I GWGCWC 5 cut(s) 1119, 1638, 1717, 1954, 2285
Alw26I GTCTC 5 cut(s) 642, 787, 1153, 2404, 2985
AlwI GGATC 4 cut(s) 876, 1333, 1545, 2476
AlwNI CAGNNNCTG 2 cut(s) 1232, 2630
Ama87I CYCGRG 1 cut(s) 2999
Aor13HI TCCGGA 2 cut(s) 1062, 1569
AoxI GGCC 7 cut(s) 1287, 1505, 1791, 2022, 2061, 2583, 3003
ApoI RAATTY 4 cut(s) 188, 956, 1317, 2267
AseI ATTAAT 1 cut(s) 1305
AspS9I GGNCC 5 cut(s) 345, 1939, 2084, 2210, 2500
AsuHPI GGTGA 8 cut(s) 518, 751, 906, 1741, 1937, 2273, 2512, 2561
AsuII TTCGAA 1 cut(s) 2863
AvaI CYCGRG 1 cut(s) 2999
AvaII GGWCC 5 cut(s) 345, 1939, 2084, 2210, 2500
AxyI CCTNAGG 1 cut(s) 690
BanII GRGCYC 4 cut(s) 1034, 1119, 1717, 2285
BarI GAAGNNNNNNTAC 6 cut(s) 58, 90, 262, 294, 2712, 2744
BauI CACGAG 2 cut(s) 1637, 1716
BbrPI CACGTG 2 cut(s) 2300, 2318
BbsI GAAGAC 2 cut(s) 1506, 2973
Bbv12I GWGCWC 5 cut(s) 1119, 1638, 1717, 1954, 2285
BceAI ACGGC 1 cut(s) 295
BciT130I CCWGG 7 cut(s) 975, 1041, 1083, 1211, 1339, 1686, 2625
BciVI GTATCC 1 cut(s) 247
BclI TGATCA 1 cut(s) 2179
BcoDI GTCTC 5 cut(s) 642, 787, 1153, 2404, 2985
BcuI ACTAGT 1 cut(s) 404
BfmI CTRYAG 8 cut(s) 253, 825, 855, 2394, 2495, 2729, 2763, 3063
BfrI CTTAAG 1 cut(s) 305
BfuAI ACCTGC 2 cut(s) 556, 2488
BfuI GTATCC 1 cut(s) 247
BglI GCCNNNNNGGC 1 cut(s) 1595
BlpI GCTNAGC 1 cut(s) 219
BmcAI AGTACT 1 cut(s) 517
Bme1390I CCNGG 7 cut(s) 975, 1041, 1083, 1211, 1339, 1686, 2625
Bme18I GGWCC 5 cut(s) 345, 1939, 2084, 2210, 2500
BmeT110I CYCGRG 1 cut(s) 2999
BmgBI CACGTC 1 cut(s) 2801
BmgT120I GGNCC 5 cut(s) 345, 1939, 2084, 2210, 2500
BmiI GGNNCC 4 cut(s) 346, 1061, 2116, 2212
BmrFI CCNGG 7 cut(s) 975, 1041, 1083, 1211, 1339, 1686, 2625
BmrI ACTGGG 1 cut(s) 1427
BmsI GCATC 2 cut(s) 265, 1213
BmuI ACTGGG 1 cut(s) 1427
BpiI GAAGAC 2 cut(s) 1506, 2973
BplI GAGNNNNNCTC 2 cut(s) 2982, 3014
BpmI CTGGAG 4 cut(s) 1104, 1193, 1321, 2607
Bpu10I CCTNAGC 1 cut(s) 2352
Bpu1102I GCTNAGC 1 cut(s) 219
Bpu14I TTCGAA 1 cut(s) 2863
BpuEI CTTGAG 2 cut(s) 981, 2493
BsaAI YACGTR 2 cut(s) 2300, 2318
BsaBI GATNNNNATC 1 cut(s) 2571
BsaHI GRCGYC 3 cut(s) 393, 1929, 3044
BsaI GGTCTC 1 cut(s) 787
BsaJI CCNNGG 6 cut(s) 612, 974, 1026, 1338, 1593, 3054
BsaWI WCCGGW 2 cut(s) 1062, 1569
BsaXI ACNNNNNCTCC 8 cut(s) 639, 669, 748, 778, 817, 847, 1713, 1743
Bsc4I CCNNNNNNNGG 9 cut(s) 341, 342, 503, 728, 1028, 1438, 1649, 2125, 2258
Bse118I RCCGGY 1 cut(s) 2063
Bse1I ACTGG 6 cut(s) 323, 528, 590, 913, 1433, 2222
Bse21I CCTNAGG 1 cut(s) 690
Bse3DI GCAATG 4 cut(s) 235, 1713, 2327, 2721
Bse8I GATNNNNATC 1 cut(s) 2571
BseAI TCCGGA 2 cut(s) 1062, 1569
BseBI CCWGG 7 cut(s) 975, 1041, 1083, 1211, 1339, 1686, 2625
BseDI CCNNGG 6 cut(s) 612, 974, 1026, 1338, 1593, 3054
BseGI GGATG 4 cut(s) 89, 424, 1566, 1861
BseJI GATNNNNATC 1 cut(s) 2571
BseLI CCNNNNNNNGG 9 cut(s) 341, 342, 503, 728, 1028, 1438, 1649, 2125, 2258
BseMI GCAATG 4 cut(s) 235, 1713, 2327, 2721
BseMII CTCAG 3 cut(s) 210, 330, 1361
BseNI ACTGG 6 cut(s) 323, 528, 590, 913, 1433, 2222
BseRI GAGGAG 2 cut(s) 822, 2999
BsgI GTGCAG 2 cut(s) 534, 2340
BshFI GGCC 7 cut(s) 1289, 1507, 1793, 2024, 2063, 2585, 3005
BsiHKAI GWGCWC 5 cut(s) 1119, 1638, 1717, 1954, 2285
BsiHKCI CYCGRG 1 cut(s) 2999
BsiSI CCGG 5 cut(s) 1063, 1570, 1910, 2064, 2208
BslFI GGGAC 4 cut(s) 298, 404, 1916, 2144
BslI CCNNNNNNNGG 9 cut(s) 341, 342, 503, 728, 1028, 1438, 1649, 2125, 2258
BsmAI GTCTC 5 cut(s) 642, 787, 1153, 2404, 2985
BsmFI GGGAC 4 cut(s) 298, 404, 1916, 2144
BsmI GAATGC 1 cut(s) 2850
BsnI GGCC 7 cut(s) 1289, 1507, 1793, 2024, 2063, 2585, 3005
Bso31I GGTCTC 1 cut(s) 787
BsoBI CYCGRG 1 cut(s) 2999
Bsp119I TTCGAA 1 cut(s) 2863
Bsp1286I GDGCHC 6 cut(s) 1034, 1119, 1638, 1717, 1954, 2285
Bsp13I TCCGGA 2 cut(s) 1062, 1569
Bsp143I GATC 7 cut(s) 868, 1325, 1537, 2179, 2458, 2468, 2572
Bsp1720I GCTNAGC 1 cut(s) 219
Bsp19I CCATGG 1 cut(s) 3054
BspACI CCGC 9 cut(s) 83, 359, 1037, 1099, 1199, 1774, 2582, 2847, 3028
BspANI GGCC 7 cut(s) 1289, 1507, 1793, 2024, 2063, 2585, 3005
BspCNI CTCAG 3 cut(s) 211, 331, 1362
BspEI TCCGGA 2 cut(s) 1062, 1569
BspLI GGNNCC 4 cut(s) 346, 1061, 2116, 2212
BspMAI CTGCAG 4 cut(s) 2398, 2499, 2767, 3067
BspMI ACCTGC 2 cut(s) 556, 2488
BspPI GGATC 4 cut(s) 876, 1333, 1545, 2476
BspQI GCTCTTC 1 cut(s) 1568
BspT104I TTCGAA 1 cut(s) 2863
BspTI CTTAAG 1 cut(s) 305
BspTNI GGTCTC 1 cut(s) 787
BsrDI GCAATG 4 cut(s) 235, 1713, 2327, 2721
BsrFI RCCGGY 1 cut(s) 2063
BsrI ACTGG 6 cut(s) 323, 528, 590, 913, 1433, 2222
BssAI RCCGGY 1 cut(s) 2063
BssECI CCNNGG 6 cut(s) 612, 974, 1026, 1338, 1593, 3054
BssMI GATC 7 cut(s) 868, 1325, 1537, 2179, 2458, 2468, 2572
BssNI GRCGYC 3 cut(s) 393, 1929, 3044
BssSI CACGAG 2 cut(s) 1637, 1716
BssT1I CCWWGG 3 cut(s) 612, 1026, 3054
Bst2BI CACGAG 2 cut(s) 1637, 1716
Bst2UI CCWGG 7 cut(s) 975, 1041, 1083, 1211, 1339, 1686, 2625
Bst4CI ACNGT 5 cut(s) 655, 706, 1990, 2053, 3016
Bst6I CTCTTC 4 cut(s) 420, 623, 1568, 3033
BstACI GRCGYC 3 cut(s) 393, 1929, 3044
BstAFI CTTAAG 1 cut(s) 305
BstAPI GCANNNNNTGC 1 cut(s) 2494
BstBAI YACGTR 2 cut(s) 2300, 2318
BstBI TTCGAA 1 cut(s) 2863
BstC8I GCNNGC 8 cut(s) 23, 81, 107, 1189, 2359, 2415, 2490, 2587
BstDEI CTNAG 8 cut(s) 132, 219, 339, 519, 690, 1370, 2120, 2352
BstDSI CCRYGG 1 cut(s) 3054
BstENI CCTNNNNNAGG 1 cut(s) 2123
BstF5I GGATG 4 cut(s) 89, 424, 1566, 1861
BstKTI GATC 7 cut(s) 871, 1328, 1540, 2182, 2461, 2471, 2575
BstMAI GTCTC 5 cut(s) 642, 787, 1153, 2404, 2985
BstMBI GATC 7 cut(s) 868, 1325, 1537, 2179, 2458, 2468, 2572
BstNI CCWGG 7 cut(s) 975, 1041, 1083, 1211, 1339, 1686, 2625
BstNSI RCATGY 2 cut(s) 25, 1273
BstSCI CCNGG 7 cut(s) 973, 1039, 1081, 1209, 1337, 1684, 2623
BstSFI CTRYAG 8 cut(s) 253, 825, 855, 2394, 2495, 2729, 2763, 3063
BstV2I GAAGAC 2 cut(s) 1506, 2973
BstX2I RGATCY 3 cut(s) 868, 1325, 2468
BstXI CCANNNNNNTGG 1 cut(s) 2602
BstYI RGATCY 3 cut(s) 868, 1325, 2468
Bsu36I CCTNAGG 1 cut(s) 690
BsuI GTATCC 1 cut(s) 247
BsuRI GGCC 7 cut(s) 1289, 1507, 1793, 2024, 2063, 2585, 3005
BtgI CCRYGG 1 cut(s) 3054
BtgZI GCGATG 3 cut(s) 1780, 2376, 2580
BtrI CACGTC 1 cut(s) 2801
BtsCI GGATG 4 cut(s) 89, 424, 1566, 1861
BtsI GCAGTG 4 cut(s) 900, 2794, 2800, 3072
BtsIMutI CAGTG 6 cut(s) 900, 906, 1119, 2794, 2800, 3072
BveI ACCTGC 2 cut(s) 556, 2488
Cac8I GCNNGC 8 cut(s) 23, 81, 107, 1189, 2359, 2415, 2490, 2587
CaiI CAGNNNCTG 2 cut(s) 1232, 2630
Cfr10I RCCGGY 1 cut(s) 2063
Cfr13I GGNCC 5 cut(s) 345, 1939, 2084, 2210, 2500
CseI GACGC 2 cut(s) 401, 3033
Csp6I GTAC 6 cut(s) 516, 609, 1333, 2165, 2705, 2879
CviQI GTAC 6 cut(s) 516, 609, 1333, 2165, 2705, 2879
DdeI CTNAG 8 cut(s) 132, 219, 339, 519, 690, 1370, 2120, 2352
DpnI GATC 7 cut(s) 870, 1327, 1539, 2181, 2460, 2470, 2574
DpnII GATC 7 cut(s) 868, 1325, 1537, 2179, 2458, 2468, 2572
DraI TTTAAA 1 cut(s) 2904
EaeI YGGCCR 1 cut(s) 2583
Eam1104I CTCTTC 4 cut(s) 420, 623, 1568, 3033
EarI CTCTTC 4 cut(s) 420, 623, 1568, 3033
EciI GGCGGA 3 cut(s) 1026, 1114, 1763
Ecl136II GAGCTC 3 cut(s) 1117, 1715, 2283
Eco130I CCWWGG 3 cut(s) 612, 1026, 3054
Eco147I AGGCCT 2 cut(s) 1507, 3005
Eco24I GRGCYC 4 cut(s) 1034, 1119, 1717, 2285
Eco31I GGTCTC 1 cut(s) 787
Eco47I GGWCC 5 cut(s) 345, 1939, 2084, 2210, 2500
Eco53kI GAGCTC 3 cut(s) 1117, 1715, 2283
Eco57I CTGAAG 4 cut(s) 558, 884, 2447, 2471
Eco72I CACGTG 2 cut(s) 2300, 2318
Eco81I CCTNAGG 1 cut(s) 690
Eco88I CYCGRG 1 cut(s) 2999
EcoICRI GAGCTC 3 cut(s) 1117, 1715, 2283
EcoNI CCTNNNNNAGG 1 cut(s) 2123
EcoO109I RGGNCCY 2 cut(s) 2084, 2500
EcoRII CCWGG 7 cut(s) 973, 1039, 1081, 1209, 1337, 1684, 2623
EcoT14I CCWWGG 3 cut(s) 612, 1026, 3054
EcoT38I GRGCYC 4 cut(s) 1034, 1119, 1717, 2285
ErhI CCWWGG 3 cut(s) 612, 1026, 3054
FaqI GGGAC 4 cut(s) 298, 404, 1916, 2144
FauI CCCGC 1 cut(s) 76
FbaI TGATCA 1 cut(s) 2179
FokI GGATG 4 cut(s) 76, 431, 1553, 1868
FriOI GRGCYC 4 cut(s) 1034, 1119, 1717, 2285
GsuI CTGGAG 4 cut(s) 1104, 1193, 1321, 2607
HaeIII GGCC 7 cut(s) 1289, 1507, 1793, 2024, 2063, 2585, 3005
HapII CCGG 5 cut(s) 1063, 1570, 1910, 2064, 2208
HgaI GACGC 2 cut(s) 401, 3033
Hin1I GRCGYC 3 cut(s) 393, 1929, 3044
HincII GTYRAC 1 cut(s) 1429
HindII GTYRAC 1 cut(s) 1429
HpaII CCGG 5 cut(s) 1063, 1570, 1910, 2064, 2208
HphI GGTGA 8 cut(s) 518, 751, 906, 1741, 1937, 2273, 2512, 2561
Hpy166II GTNNAC 5 cut(s) 595, 914, 1429, 2557, 2798
Hpy8I GTNNAC 5 cut(s) 595, 914, 1429, 2557, 2798
Hpy99I CGWCG 1 cut(s) 2978
HpyCH4III ACNGT 5 cut(s) 655, 706, 1990, 2053, 3016
HpyCH4IV ACGT 4 cut(s) 1929, 2299, 2317, 2800
HpyF3I CTNAG 8 cut(s) 132, 219, 339, 519, 690, 1370, 2120, 2352
HpySE526I ACGT 4 cut(s) 1929, 2299, 2317, 2800
Hsp92I GRCGYC 3 cut(s) 393, 1929, 3044
Kpn2I TCCGGA 2 cut(s) 1062, 1569
Ksp22I TGATCA 1 cut(s) 2179
Kzo9I GATC 7 cut(s) 868, 1325, 1537, 2179, 2458, 2468, 2572
LguI GCTCTTC 1 cut(s) 1568
LweI GCATC 2 cut(s) 265, 1213
MaeII ACGT 4 cut(s) 1929, 2299, 2317, 2800
MalI GATC 7 cut(s) 870, 1327, 1539, 2181, 2460, 2470, 2574
MboI GATC 7 cut(s) 868, 1325, 1537, 2179, 2458, 2468, 2572
MfeI CAATTG 2 cut(s) 1644, 2754
MflI RGATCY 3 cut(s) 868, 1325, 2468
MhlI GDGCHC 6 cut(s) 1034, 1119, 1638, 1717, 1954, 2285
MlyI GAGTC 7 cut(s) 461, 758, 1200, 1884, 2321, 2718, 3090
MroI TCCGGA 2 cut(s) 1062, 1569
MslI CAYNNNNRTG 4 cut(s) 228, 1523, 1751, 2886
MspA1I CMGCKG 2 cut(s) 662, 3062
MspCI CTTAAG 1 cut(s) 305
MspI CCGG 5 cut(s) 1063, 1570, 1910, 2064, 2208
MspR9I CCNGG 7 cut(s) 975, 1041, 1083, 1211, 1339, 1686, 2625
MunI CAATTG 2 cut(s) 1644, 2754
Mva1269I GAATGC 1 cut(s) 2850
MvaI CCWGG 7 cut(s) 975, 1041, 1083, 1211, 1339, 1686, 2625
NcoI CCATGG 1 cut(s) 3054
NdeII GATC 7 cut(s) 868, 1325, 1537, 2179, 2458, 2468, 2572
NlaIV GGNNCC 4 cut(s) 346, 1061, 2116, 2212
NmeAIII GCCGAG 1 cut(s) 2381
NmuCI GTSAC 6 cut(s) 196, 453, 1729, 1925, 2313, 2518
NspI RCATGY 2 cut(s) 25, 1273
NspV TTCGAA 1 cut(s) 2863
PaeI GCATGC 1 cut(s) 25
PaeR7I CTCGAG 1 cut(s) 2999
PceI AGGCCT 2 cut(s) 1507, 3005
PciSI GCTCTTC 1 cut(s) 1568
PctI GAATGC 1 cut(s) 2850
PflMI CCANNNNNTGG 1 cut(s) 1649
PfoI TCCNGGA 1 cut(s) 1684
PleI GAGTC 7 cut(s) 460, 757, 1200, 1883, 2320, 2718, 3089
PmaCI CACGTG 2 cut(s) 2300, 2318
PmlI CACGTG 2 cut(s) 2300, 2318
PpsI GAGTC 7 cut(s) 460, 757, 1200, 1883, 2320, 2718, 3089
Ppu21I YACGTR 2 cut(s) 2300, 2318
PpuMI RGGWCCY 2 cut(s) 2084, 2500
PshBI ATTAAT 1 cut(s) 1305
Psp124BI GAGCTC 3 cut(s) 1119, 1717, 2285
Psp5II RGGWCCY 2 cut(s) 2084, 2500
Psp6I CCWGG 7 cut(s) 973, 1039, 1081, 1209, 1337, 1684, 2623
PspCI CACGTG 2 cut(s) 2300, 2318
PspGI CCWGG 7 cut(s) 973, 1039, 1081, 1209, 1337, 1684, 2623
PspN4I GGNNCC 4 cut(s) 346, 1061, 2116, 2212
PspPI GGNCC 5 cut(s) 345, 1939, 2084, 2210, 2500
PspPPI RGGWCCY 2 cut(s) 2084, 2500
PstI CTGCAG 4 cut(s) 2398, 2499, 2767, 3067
PstNI CAGNNNCTG 2 cut(s) 1232, 2630
PsuI RGATCY 3 cut(s) 868, 1325, 2468
PvuII CAGCTG 2 cut(s) 662, 3062
RsaI GTAC 6 cut(s) 517, 610, 1334, 2166, 2706, 2880
RsaNI GTAC 6 cut(s) 516, 609, 1333, 2165, 2705, 2879
RseI CAYNNNNRTG 4 cut(s) 228, 1523, 1751, 2886
SacI GAGCTC 3 cut(s) 1119, 1717, 2285
SapI GCTCTTC 1 cut(s) 1568
Sau3AI GATC 7 cut(s) 868, 1325, 1537, 2179, 2458, 2468, 2572
Sau96I GGNCC 5 cut(s) 345, 1939, 2084, 2210, 2500
SbfI CCTGCAGG 1 cut(s) 2499
ScaI AGTACT 1 cut(s) 517
SchI GAGTC 7 cut(s) 461, 758, 1200, 1884, 2321, 2718, 3090
ScrFI CCNGG 7 cut(s) 975, 1041, 1083, 1211, 1339, 1686, 2625
SdaI CCTGCAGG 1 cut(s) 2499
SduI GDGCHC 6 cut(s) 1034, 1119, 1638, 1717, 1954, 2285
SfaNI GCATC 2 cut(s) 265, 1213
SfcI CTRYAG 8 cut(s) 253, 825, 855, 2394, 2495, 2729, 2763, 3063
Sfr274I CTCGAG 1 cut(s) 2999
SfuI TTCGAA 1 cut(s) 2863
SinI GGWCC 5 cut(s) 345, 1939, 2084, 2210, 2500
SlaI CTCGAG 1 cut(s) 2999
SmiMI CAYNNNNRTG 4 cut(s) 228, 1523, 1751, 2886
SmlI CTYRAG 4 cut(s) 305, 996, 2508, 2999
SmoI CTYRAG 4 cut(s) 305, 996, 2508, 2999
SpeI ACTAGT 1 cut(s) 404
SphI GCATGC 1 cut(s) 25
Sse8387I CCTGCAGG 1 cut(s) 2499
SseBI AGGCCT 2 cut(s) 1507, 3005
SsiI CCGC 9 cut(s) 83, 359, 1037, 1099, 1199, 1774, 2582, 2847, 3028
SspI AATATT 1 cut(s) 2443
SstI GAGCTC 3 cut(s) 1119, 1717, 2285
StuI AGGCCT 2 cut(s) 1507, 3005
StyD4I CCNGG 7 cut(s) 973, 1039, 1081, 1209, 1337, 1684, 2623
StyI CCWWGG 3 cut(s) 612, 1026, 3054
TaaI ACNGT 5 cut(s) 655, 706, 1990, 2053, 3016
TaiI ACGT 4 cut(s) 1932, 2302, 2320, 2803
TaqI TCGA 7 cut(s) 512, 599, 981, 1473, 2863, 3000, 3084
TaqII GACCGA 1 cut(s) 184
TatI WGTACW 3 cut(s) 515, 1332, 2878
TauI GCSGC 3 cut(s) 1202, 2585, 2850
TscAI CASTG 6 cut(s) 900, 913, 1126, 2794, 2800, 3072
TseFI GTSAC 6 cut(s) 196, 453, 1729, 1925, 2313, 2518
Tsp45I GTSAC 6 cut(s) 196, 453, 1729, 1925, 2313, 2518
TspDTI ATGAA 7 cut(s) 78, 1323, 1491, 2217, 2280, 2352, 2838
TspRI CASTG 6 cut(s) 900, 913, 1126, 2794, 2800, 3072
Van91I CCANNNNNTGG 1 cut(s) 1649
Vha464I CTTAAG 1 cut(s) 305
VpaK11BI GGWCC 5 cut(s) 345, 1939, 2084, 2210, 2500
VspI ATTAAT 1 cut(s) 1305
XagI CCTNNNNNAGG 1 cut(s) 2123
XapI RAATTY 4 cut(s) 188, 956, 1317, 2267
XbaI TCTAGA 1 cut(s) 1249
XceI RCATGY 2 cut(s) 25, 1273
XhoI CTCGAG 1 cut(s) 2999
ZraI GACGTC 1 cut(s) 1930
ZrmI AGTACT 1 cut(s) 517
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.