Rh5BG282400

GDSL esterase lipase At4g26790-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0011664
Physical Location & Seq
Reverse (-)
35750766 .. 35752505
1740 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG282400.1

Sequence Viewer

Length: 1050 bp
ATGGCATTTAAAGCAATTTATTGGTTTCTCCTAACCCACTTCCTCCTGCAAATTTCTAGGGTGTATGCAAGAGTTCCGGCAATCATTGTGTTTGGAGACTCTTCGGTTGATGCAGGAAACAATAACCAGGTATCGACAATCCTAAAGAGCGACTTTCCACCCTACGGTCGTGATTTCTATGGTGGACAGCCTACAGGGAGATTTTCCAATGGTAGGATTCCACCAGACTTCATTTCTGAAGCTTTTGGGATCAAGCCATTGATACCTGCATTTTTGGATCCATTTTATAACATTAAGGATTTTGCTACTGGAGTTTGCTTTGCCTCCGCAGGAACTGGTTATGATAATACCACTTCTGATGTCCTATCTGTGATACCTCTTTGGAAGGAATTGGAGTACTACGAAGAATACCAGCAGGACCTCAGAATCTATCTAGGAAATGATGAAGCAAACGAGATTCTAGGAGAAGCACTTTACTTAATGAGTATAGGAACTAATGATTTCCTAGAAAACTACTATATATTATCAAACAGATCATCGGAGTATTCCACAGAGGAGTACCAGAATTTTCTAGCAAAAATTGCTGGAAATTTTATCACAGAGCTCTTCCAGCTTGGAGCTCGAAAGATATCTCTAGGCGGCCTTCCTCCAATGGGTTGTCTGCCATTGGAGAGAACTAGAAATCTTCTTTTGGGGAGTGATTGTGTTGAGACGTACAATGATGTGGCTAGGAGTTTCAATAACAAGTTGGAGGAATTAGTTGACAGACTGAACGGGGAACTAGTTGGAATCCAGCTTGTGCTTGCAAACCCATACTATATTCTCTCGGATATCATACAAAACCCTGAATCCTTTGGATTTGAAGAAGCAGCAACAGCTTGTTGTGGTACAGGATTGTTTGAGATGGGTTACATGTGTACAAAGATAAACCCATTTACGTGTTCAGATGCAAATCAATATGTATTTTGGGATGCCTTCCACCCCACAGAGAGAACAAATGGTATTGTTGCAGATCATGTATTTAAAACTTGTCTAGCTCAATTTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

349

Amino Acids

39.2

Weight (kDa)

4.49

Isoelectric Point (pI)

31.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 28 - 340 8.2e-32 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011664)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G26790 AT4G26790
fragaria_vesca FvH4_3g23490 FvH4_3g23490 FvH4_3g23490
malus_domestica MD11G1210200.v1.1
prunus_persica Prupe.4G222900_v2.0.a1
pyrus_communis pycom11g18580
rosa_chinensis RchiOBHm_Chr5g0041341
rosa_laevigata RLG00000034054
rosa_multiflora Rmu_sc0000594.1_g000072
rosa_roxburghii Rroxscaffold_1G00039290
rosa_rugosa Rorug05G0191600 Rorug05G0191700
rosa_samantha Rh5AG277700 Rh5BG282400 Rh5CG314800 Rh5DG291200
rosa_wichuraiana Rw5G026140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 288
Acc36I ACCTGC 1 cut(s) 274
AciI CCGC 2 cut(s) 327, 639
AclWI GGATC 3 cut(s) 257, 272, 285
AcsI RAATTY 3 cut(s) 51, 565, 589
AcuI CTGAAG 1 cut(s) 258
AfaI GTAC 5 cut(s) 398, 560, 716, 889, 919
AfiI CCNNNNNNNGG 3 cut(s) 164, 213, 653
AflIII ACRYGT 2 cut(s) 912, 938
AgsI TTSAA 2 cut(s) 739, 863
AhlI ACTAGT 1 cut(s) 781
AjnI CCWGG 1 cut(s) 126
AluBI AGCT 7 cut(s) 242, 604, 613, 620, 796, 878, 1037
AluI AGCT 7 cut(s) 242, 604, 613, 620, 796, 878, 1037
Alw21I GWGCWC 2 cut(s) 606, 622
Alw26I GTCTC 2 cut(s) 90, 704
AlwI GGATC 3 cut(s) 257, 272, 285
AlwNI CAGNNNCTG 1 cut(s) 335
AoxI GGCC 1 cut(s) 640
ApeKI GCWGC 1 cut(s) 869
ApoI RAATTY 3 cut(s) 51, 565, 589
AspS9I GGNCC 1 cut(s) 418
AvaII GGWCC 1 cut(s) 418
BamHI GGATCC 1 cut(s) 277
BanII GRGCYC 2 cut(s) 606, 622
Bbv12I GWGCWC 2 cut(s) 606, 622
BbvI GCAGC 1 cut(s) 881
BccI CCATC 1 cut(s) 898
BciT130I CCWGG 1 cut(s) 128
BcoDI GTCTC 2 cut(s) 90, 704
BcuI ACTAGT 1 cut(s) 781
BfmI CTRYAG 1 cut(s) 192
BfuAI ACCTGC 1 cut(s) 274
BisI GCNGC 2 cut(s) 640, 870
BlsI GCNGC 2 cut(s) 641, 871
BmcAI AGTACT 1 cut(s) 398
Bme1390I CCNGG 1 cut(s) 128
Bme18I GGWCC 1 cut(s) 418
BmgT120I GGNCC 1 cut(s) 418
BmiI GGNNCC 1 cut(s) 279
BmrFI CCNGG 1 cut(s) 128
BmsI GCATC 3 cut(s) 100, 937, 961
BpmI CTGGAG 1 cut(s) 330
BsaAI YACGTR 1 cut(s) 939
BsaBI GATNNNNATC 1 cut(s) 951
BsaXI ACNNNNNCTCC 2 cut(s) 688, 718
Bsc4I CCNNNNNNNGG 3 cut(s) 164, 213, 653
Bse1I ACTGG 2 cut(s) 313, 340
Bse8I GATNNNNATC 1 cut(s) 951
BseBI CCWGG 1 cut(s) 128
BseGI GGATG 1 cut(s) 976
BseJI GATNNNNATC 1 cut(s) 951
BseLI CCNNNNNNNGG 3 cut(s) 164, 213, 653
BseMII CTCAG 1 cut(s) 436
BseNI ACTGG 2 cut(s) 313, 340
BseRI GAGGAG 1 cut(s) 569
BseXI GCAGC 1 cut(s) 881
Bsh1285I CGRYCG 1 cut(s) 169
BshFI GGCC 1 cut(s) 642
BsiEI CGRYCG 1 cut(s) 169
BsiHKAI GWGCWC 2 cut(s) 606, 622
BsiSI CCGG 1 cut(s) 77
BslI CCNNNNNNNGG 3 cut(s) 164, 213, 653
BsmAI GTCTC 2 cut(s) 90, 704
BsmBI CGTCTC 1 cut(s) 704
BsnI GGCC 1 cut(s) 642
Bsp1286I GDGCHC 2 cut(s) 606, 622
Bsp1407I TGTACA 1 cut(s) 917
Bsp143I GATC 4 cut(s) 249, 277, 533, 1012
BspACI CCGC 2 cut(s) 327, 639
BspANI GGCC 1 cut(s) 642
BspCNI CTCAG 1 cut(s) 435
BspLI GGNNCC 1 cut(s) 279
BspMI ACCTGC 1 cut(s) 274
BspPI GGATC 3 cut(s) 257, 272, 285
BspQI GCTCTTC 1 cut(s) 611
BsrGI TGTACA 1 cut(s) 917
BsrI ACTGG 2 cut(s) 313, 340
BssMI GATC 4 cut(s) 249, 277, 533, 1012
Bst2UI CCWGG 1 cut(s) 128
Bst4CI ACNGT 1 cut(s) 167
Bst6I CTCTTC 2 cut(s) 106, 611
BstAPI GCANNNNNTGC 1 cut(s) 581
BstAUI TGTACA 1 cut(s) 917
BstBAI YACGTR 1 cut(s) 939
BstC8I GCNNGC 1 cut(s) 804
BstDEI CTNAG 1 cut(s) 422
BstF5I GGATG 1 cut(s) 976
BstKTI GATC 4 cut(s) 252, 280, 536, 1015
BstMAI GTCTC 2 cut(s) 90, 704
BstMBI GATC 4 cut(s) 249, 277, 533, 1012
BstMCI CGRYCG 1 cut(s) 169
BstMWI GCNNNNNNNGC 4 cut(s) 11, 581, 610, 875
BstNI CCWGG 1 cut(s) 128
BstNSI RCATGY 1 cut(s) 916
BstSCI CCNGG 1 cut(s) 126
BstSFI CTRYAG 1 cut(s) 192
BstV1I GCAGC 1 cut(s) 881
BstX2I RGATCY 1 cut(s) 277
BstYI RGATCY 1 cut(s) 277
BsuRI GGCC 1 cut(s) 642
BtsCI GGATG 1 cut(s) 976
BveI ACCTGC 1 cut(s) 274
Cac8I GCNNGC 1 cut(s) 804
CaiI CAGNNNCTG 1 cut(s) 335
Cfr13I GGNCC 1 cut(s) 418
CsiI ACCWGGT 1 cut(s) 126
Csp6I GTAC 5 cut(s) 397, 559, 715, 888, 918
CviAII CATG 2 cut(s) 913, 1016
CviQI GTAC 5 cut(s) 397, 559, 715, 888, 918
DdeI CTNAG 1 cut(s) 422
DpnI GATC 4 cut(s) 251, 279, 535, 1014
DpnII GATC 4 cut(s) 249, 277, 533, 1012
DraI TTTAAA 2 cut(s) 10, 1024
Eam1104I CTCTTC 2 cut(s) 106, 611
EarI CTCTTC 2 cut(s) 106, 611
Ecl136II GAGCTC 2 cut(s) 604, 620
Eco24I GRGCYC 2 cut(s) 606, 622
Eco32I GATATC 2 cut(s) 630, 832
Eco47I GGWCC 1 cut(s) 418
Eco53kI GAGCTC 2 cut(s) 604, 620
Eco57I CTGAAG 1 cut(s) 258
EcoICRI GAGCTC 2 cut(s) 604, 620
EcoO109I RGGNCCY 1 cut(s) 418
EcoRII CCWGG 1 cut(s) 126
EcoRV GATATC 2 cut(s) 630, 832
EcoT38I GRGCYC 2 cut(s) 606, 622
Esp3I CGTCTC 1 cut(s) 704
FaeI CATG 2 cut(s) 916, 1019
FalI AAGNNNNNCTT 2 cut(s) 137, 169
FatI CATG 2 cut(s) 912, 1015
Fnu4HI GCNGC 2 cut(s) 640, 870
FokI GGATG 1 cut(s) 983
FriOI GRGCYC 2 cut(s) 606, 622
Fsp4HI GCNGC 2 cut(s) 640, 870
GluI GCNGC 2 cut(s) 640, 870
GsuI CTGGAG 1 cut(s) 330
HaeIII GGCC 1 cut(s) 642
HapII CCGG 1 cut(s) 77
Hin1II CATG 2 cut(s) 916, 1019
HincII GTYRAC 1 cut(s) 763
HindII GTYRAC 1 cut(s) 763
HindIII AAGCTT 1 cut(s) 240
HinfI GANTC 6 cut(s) 98, 217, 426, 457, 789, 848
HpaII CCGG 1 cut(s) 77
Hpy166II GTNNAC 3 cut(s) 185, 763, 918
Hpy188I TCNGA 6 cut(s) 238, 358, 425, 541, 829, 946
Hpy188III TCNNGA 1 cut(s) 170
Hpy8I GTNNAC 3 cut(s) 185, 763, 918
HpyAV CCTTC 3 cut(s) 379, 653, 985
HpyCH4III ACNGT 1 cut(s) 167
HpyCH4IV ACGT 2 cut(s) 713, 938
HpyCH4V TGCA 7 cut(s) 49, 68, 113, 269, 806, 950, 1010
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 581, 610, 875
HpyF3I CTNAG 1 cut(s) 422
HpySE526I ACGT 2 cut(s) 713, 938
Hsp92II CATG 2 cut(s) 916, 1019
Kzo9I GATC 4 cut(s) 249, 277, 533, 1012
LguI GCTCTTC 1 cut(s) 611
LmnI GCTCC 1 cut(s) 617
Lsp1109I GCAGC 1 cut(s) 881
LweI GCATC 3 cut(s) 100, 937, 961
MabI ACCWGGT 1 cut(s) 126
MaeII ACGT 2 cut(s) 713, 938
MaeIII GTNAC 1 cut(s) 908
MalI GATC 4 cut(s) 251, 279, 535, 1014
MboI GATC 4 cut(s) 249, 277, 533, 1012
MboII GAAGA 5 cut(s) 93, 416, 598, 677, 875
MflI RGATCY 1 cut(s) 277
MhlI GDGCHC 2 cut(s) 606, 622
MluCI AATT 8 cut(s) 15, 51, 389, 565, 579, 589, 755, 1040
MlyI GAGTC 1 cut(s) 92
MmeI TCCRAC 2 cut(s) 729, 766
MnlI CCTC 7 cut(s) 53, 334, 387, 431, 547, 657, 745
MseI TTAA 4 cut(s) 9, 294, 479, 1023
MslI CAYNNNNRTG 1 cut(s) 937
MspI CCGG 1 cut(s) 77
MspR9I CCNGG 1 cut(s) 128
MvaI CCWGG 1 cut(s) 128
MwoI GCNNNNNNNGC 4 cut(s) 11, 581, 610, 875
NdeII GATC 4 cut(s) 249, 277, 533, 1012
NlaIII CATG 2 cut(s) 916, 1019
NlaIV GGNNCC 1 cut(s) 279
NspI RCATGY 1 cut(s) 916
PciI ACATGT 1 cut(s) 912
PciSI GCTCTTC 1 cut(s) 611
PfeI GAWTC 5 cut(s) 217, 426, 457, 789, 848
PkrI GCNGC 2 cut(s) 641, 871
PleI GAGTC 1 cut(s) 92
PpsI GAGTC 1 cut(s) 92
Ppu21I YACGTR 1 cut(s) 939
PpuMI RGGWCCY 1 cut(s) 418
PscI ACATGT 1 cut(s) 912
PsiI TTATAA 1 cut(s) 288
Psp124BI GAGCTC 2 cut(s) 606, 622
Psp5II RGGWCCY 1 cut(s) 418
Psp6I CCWGG 1 cut(s) 126
PspGI CCWGG 1 cut(s) 126
PspN4I GGNNCC 1 cut(s) 279
PspPI GGNCC 1 cut(s) 418
PspPPI RGGWCCY 1 cut(s) 418
PstNI CAGNNNCTG 1 cut(s) 335
PsuI RGATCY 1 cut(s) 277
RsaI GTAC 5 cut(s) 398, 560, 716, 889, 919
RsaNI GTAC 5 cut(s) 397, 559, 715, 888, 918
RseI CAYNNNNRTG 1 cut(s) 937
SacI GAGCTC 2 cut(s) 606, 622
SapI GCTCTTC 1 cut(s) 611
SaqAI TTAA 4 cut(s) 9, 294, 479, 1023
SatI GCNGC 2 cut(s) 640, 870
Sau3AI GATC 4 cut(s) 249, 277, 533, 1012
Sau96I GGNCC 1 cut(s) 418
ScaI AGTACT 1 cut(s) 398
SchI GAGTC 1 cut(s) 92
ScrFI CCNGG 1 cut(s) 128
SduI GDGCHC 2 cut(s) 606, 622
SexAI ACCWGGT 1 cut(s) 126
SfaNI GCATC 3 cut(s) 100, 937, 961
SfcI CTRYAG 1 cut(s) 192
SinI GGWCC 1 cut(s) 418
SmiMI CAYNNNNRTG 1 cut(s) 937
SpeI ACTAGT 1 cut(s) 781
Sse9I AATT 8 cut(s) 15, 51, 389, 565, 579, 589, 755, 1040
SsiI CCGC 2 cut(s) 327, 639
SstI GAGCTC 2 cut(s) 606, 622
StyD4I CCNGG 1 cut(s) 126
TaaI ACNGT 1 cut(s) 167
TaiI ACGT 2 cut(s) 716, 941
TaqI TCGA 2 cut(s) 134, 622
TasI AATT 8 cut(s) 15, 51, 389, 565, 579, 589, 755, 1040
TatI WGTACW 2 cut(s) 396, 917
TauI GCSGC 1 cut(s) 642
TfiI GAWTC 5 cut(s) 217, 426, 457, 789, 848
Tru1I TTAA 4 cut(s) 9, 294, 479, 1023
Tru9I TTAA 4 cut(s) 9, 294, 479, 1023
TseI GCWGC 1 cut(s) 869
TspDTI ATGAA 2 cut(s) 220, 459
VpaK11BI GGWCC 1 cut(s) 418
XapI RAATTY 3 cut(s) 51, 565, 589
XceI RCATGY 1 cut(s) 916
ZrmI AGTACT 1 cut(s) 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.