MD11G1222300.v1.1
ERF Family

Belongs to the calycin superfamily. Lipocalin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0009620
Physical Location & Seq
Reverse (-)
32561048 .. 32564443
3396 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1222300.v1.1.491

Sequence Viewer

Length: 990 bp
ATGGTTCTTGTGCTCCAATGCTCTCTTCCTCCTCCTCCAAATGTCTCCACCAGCAGGAGAGTGCCTGGTAAGATAATGATGAGTTGCTGTTTGGAGCAATGTATCAGCAGCAAGCCTCTGACCAGGCAGGTTTTATCCGGGCTTGCTGCAACGTTAGTGTTCATCTCTCAAACGAACCAGGCTGTTGCATTAGATGTTTCCCATCAACGAAATATATATGAGTTTGCAAATGCTTCGGAGAAGGCACTTACTCTTCCGCTCGATAAGGGATCCGATGAAGGAGATGAGAGGCTAATGATGATGAGAGGCATGACAGCAAAGAACTTTGACCCTATTAGATATTCTGGAAGGTGGTTTGAAGTTGCTTCGCTTAAACGCGGATTTGCTGGGCAAGGTCAGGAAGACTGCCACTGCACCCAGGGTGTATATACATTTGACTTGACGAAACGGGCCATCCAGGTTGATACCTTCTGTGTTCATGGGAGCCCTGATGGATATATTACTGGCATACGGGGAAATGTTCAATGCCTTTCGGACGAAGATTTGGAGAAAAAAGAAACGGATCTAGAAAAGCAGGAGATGATCACAGAGAAGTGTTACCTTCGTTTTCCAACGTTGCCATTTATCCCTAAGCTGCCATATGATGTGATTGACACTGATTATGACAATTACGCTCTTGTTTCGGGAGCGAAAGACAAGGGTTTTATACAGATTTACTCGAGAACACCTAATCCTGGTCCTGAGTTCATAGAGAAGTACAAATCTTACTTGGCCAACTTCGGATATGACCCGAGCAAAATCAAGGACACACCACAAGACTGTGAGCAAATGACAGACACGCGGTTATCTGCAATGATGTCGATGCCCGGGATGCAACAGGCATTAACAAATCAATTTCCTGATCTAGAACTGAAGAAACCTGTTCAATTTGATCCCTTTACGAGCGTATTCGACACCCTAAAGAAGCTTGTTCAGCTTTATTTTAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0001817 GO:0001818 GO:0001952 GO:0001953 GO:0002682 GO:0002683 GO:0002685 GO:0002686 GO:0003006 GO:0003674 GO:0005488 GO:0005496 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005829 GO:0005840 GO:0005975 GO:0005996 GO:0006006 GO:0006629 GO:0006950 GO:0006979 GO:0007162 GO:0007275 GO:0007399 GO:0007417 GO:0007420 GO:0007568 GO:0008150 GO:0008152 GO:0008285 GO:0008289 GO:0009266 GO:0009314 GO:0009408 GO:0009414 GO:0009415 GO:0009416 GO:0009507 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0009611 GO:0009628 GO:0009635 GO:0009636 GO:0009642 GO:0009644 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009888 GO:0009892 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010117 GO:0010154 GO:0010431 GO:0010565 GO:0010605 GO:0010640 GO:0010642 GO:0010646 GO:0010648 GO:0010810 GO:0010812 GO:0012505 GO:0014012 GO:0015485 GO:0016020 GO:0016043 GO:0019216 GO:0019217 GO:0019222 GO:0019318 GO:0021700 GO:0022008 GO:0022414 GO:0022626 GO:0023051 GO:0023057 GO:0030030 GO:0030154 GO:0030155 GO:0030182 GO:0030334 GO:0030336 GO:0030425 GO:0031099 GO:0031102 GO:0031103 GO:0031175 GO:0031323 GO:0031324 GO:0031347 GO:0031348 GO:0031976 GO:0031977 GO:0031984 GO:0032101 GO:0032102 GO:0032386 GO:0032387 GO:0032501 GO:0032502 GO:0032504 GO:0032642 GO:0032682 GO:0032879 GO:0032880 GO:0032934 GO:0032991 GO:0033157 GO:0033554 GO:0033993 GO:0034357 GO:0034442 GO:0034443 GO:0036094 GO:0036477 GO:0040007 GO:0040012 GO:0040013 GO:0042060 GO:0042127 GO:0042221 GO:0042246 GO:0042306 GO:0042308 GO:0042493 GO:0042651 GO:0042995 GO:0043005 GO:0043025 GO:0043178 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044087 GO:0044238 GO:0044281 GO:0044297 GO:0044421 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044445 GO:0044446 GO:0044463 GO:0044464 GO:0045833 GO:0045922 GO:0046320 GO:0046322 GO:0046822 GO:0046823 GO:0048316 GO:0048468 GO:0048471 GO:0048513 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048589 GO:0048608 GO:0048609 GO:0048660 GO:0048662 GO:0048666 GO:0048678 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050727 GO:0050728 GO:0050746 GO:0050748 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051051 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051223 GO:0051224 GO:0051239 GO:0051241 GO:0051246 GO:0051248 GO:0051270 GO:0051271 GO:0051716 GO:0051893 GO:0051895 GO:0055035 GO:0060255 GO:0060322 GO:0060341 GO:0060587 GO:0060588 GO:0061458 GO:0061564 GO:0062012 GO:0062014 GO:0065007 GO:0070201 GO:0071637 GO:0071638 GO:0071695 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0090087 GO:0090109 GO:0090317 GO:0097159 GO:0097305 GO:0097447 GO:0097458 GO:0120025 GO:0120036 GO:0120038 GO:1900015 GO:1900016 GO:1900180 GO:1900181 GO:1901562 GO:1901700 GO:1901888 GO:1901889 GO:1903391 GO:1903392 GO:1903827 GO:1903828 GO:1904589 GO:1904590 GO:1904950 GO:1990904 GO:2000097 GO:2000098 GO:2000145 GO:2000146 GO:2000401 GO:2000402 GO:2000404 GO:2000405
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipocalin_2

Protein Analysis

330

Amino Acids

37.15

Weight (kDa)

5.58

Isoelectric Point (pI)

36.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipocalin_2 PF08212 112 - 272 1.2e-07 Lipocalin-like domain
Lipocalin PF00061 173 - 272 2e-06 Lipocalin / cytosolic fatty-acid binding protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 118
AccBSI CCGCTC 1 cut(s) 259
AccII CGCG 2 cut(s) 378, 841
AciI CCGC 3 cut(s) 257, 378, 841
AclI AACGTT 2 cut(s) 152, 614
AclWI GGATC 4 cut(s) 264, 277, 570, 926
AcoI YGGCCR 1 cut(s) 771
AcuI CTGAAG 1 cut(s) 932
AfaI GTAC 1 cut(s) 758
AfiI CCNNNNNNNGG 2 cut(s) 54, 734
AgsI TTSAA 3 cut(s) 359, 524, 926
AjnI CCWGG 6 cut(s) 64, 122, 177, 417, 456, 733
AjuI GAANNNNNNNTTGG 2 cut(s) 9, 41
AloI GAACNNNNNNTCC 2 cut(s) 715, 747
AluBI AGCT 3 cut(s) 634, 967, 976
AluI AGCT 3 cut(s) 634, 967, 976
Alw21I GWGCWC 1 cut(s) 15
Alw26I GTCTC 1 cut(s) 49
AlwI GGATC 4 cut(s) 264, 277, 570, 926
Ama87I CYCGRG 3 cut(s) 718, 790, 866
AoxI GGCC 2 cut(s) 450, 771
ApeKI GCWGC 3 cut(s) 108, 146, 634
AspS9I GGNCC 2 cut(s) 450, 737
AsuC2I CCSGG 3 cut(s) 139, 867, 868
AvaI CYCGRG 3 cut(s) 718, 790, 866
AvaII GGWCC 1 cut(s) 737
BalI TGGCCA 1 cut(s) 773
BamHI GGATCC 1 cut(s) 269
BanII GRGCYC 1 cut(s) 488
BbsI GAAGAC 1 cut(s) 408
Bbv12I GWGCWC 1 cut(s) 15
BbvI GCAGC 3 cut(s) 120, 133, 621
BccI CCATC 3 cut(s) 210, 461, 485
BcgI CGANNNNNNTGC 4 cut(s) 216, 250, 840, 874
BciT130I CCWGG 6 cut(s) 66, 124, 179, 419, 458, 735
BclI TGATCA 1 cut(s) 582
BcnI CCSGG 3 cut(s) 139, 867, 868
BcoDI GTCTC 1 cut(s) 49
BfaI CTAG 2 cut(s) 566, 905
BfuAI ACCTGC 1 cut(s) 118
BisI GCNGC 3 cut(s) 109, 147, 635
BlsI GCNGC 3 cut(s) 110, 148, 636
Bme1390I CCNGG 9 cut(s) 66, 124, 139, 179, 419, 458, 735, 867, 868
Bme18I GGWCC 1 cut(s) 737
BmeT110I CYCGRG 3 cut(s) 718, 790, 866
BmgT120I GGNCC 2 cut(s) 450, 737
BmiI GGNNCC 2 cut(s) 271, 485
BmrFI CCNGG 9 cut(s) 66, 124, 139, 179, 419, 458, 735, 867, 868
BmsI GCATC 2 cut(s) 852, 861
BpiI GAAGAC 1 cut(s) 408
Bpu10I CCTNAGC 1 cut(s) 630
BpuMI CCSGG 3 cut(s) 139, 867, 868
BsaJI CCNNGG 3 cut(s) 417, 418, 866
Bsc4I CCNNNNNNNGG 2 cut(s) 54, 734
Bse1I ACTGG 1 cut(s) 508
Bse3DI GCAATG 2 cut(s) 104, 858
BseBI CCWGG 6 cut(s) 66, 124, 179, 419, 458, 735
BseDI CCNNGG 3 cut(s) 417, 418, 866
BseGI GGATG 2 cut(s) 453, 876
BseLI CCNNNNNNNGG 2 cut(s) 54, 734
BseMI GCAATG 2 cut(s) 104, 858
BseMII CTCAG 1 cut(s) 732
BseNI ACTGG 1 cut(s) 508
BseRI GAGGAG 2 cut(s) 21, 24
BseXI GCAGC 3 cut(s) 120, 133, 621
BseYI CCCAGC 1 cut(s) 386
BsgI GTGCAG 1 cut(s) 397
Bsh1236I CGCG 2 cut(s) 378, 841
BshFI GGCC 2 cut(s) 452, 773
BsiHKAI GWGCWC 1 cut(s) 15
BsiHKCI CYCGRG 3 cut(s) 718, 790, 866
BsiSI CCGG 2 cut(s) 138, 867
BslI CCNNNNNNNGG 2 cut(s) 54, 734
BsmAI GTCTC 1 cut(s) 49
BsnI GGCC 2 cut(s) 452, 773
BsoBI CYCGRG 3 cut(s) 718, 790, 866
Bsp1286I GDGCHC 2 cut(s) 15, 488
Bsp143I GATC 5 cut(s) 269, 562, 582, 901, 931
BspACI CCGC 3 cut(s) 257, 378, 841
BspANI GGCC 2 cut(s) 452, 773
BspCNI CTCAG 1 cut(s) 733
BspFNI CGCG 2 cut(s) 378, 841
BspLI GGNNCC 2 cut(s) 271, 485
BspMI ACCTGC 1 cut(s) 118
BspPI GGATC 4 cut(s) 264, 277, 570, 926
BsrBI CCGCTC 1 cut(s) 259
BsrDI GCAATG 2 cut(s) 104, 858
BsrI ACTGG 1 cut(s) 508
BssECI CCNNGG 3 cut(s) 417, 418, 866
BssMI GATC 5 cut(s) 269, 562, 582, 901, 931
Bst2UI CCWGG 6 cut(s) 66, 124, 179, 419, 458, 735
Bst4CI ACNGT 1 cut(s) 821
Bst6I CTCTTC 2 cut(s) 30, 258
BstC8I GCNNGC 2 cut(s) 113, 144
BstDEI CTNAG 2 cut(s) 630, 741
BstF5I GGATG 2 cut(s) 453, 876
BstFNI CGCG 2 cut(s) 378, 841
BstKTI GATC 5 cut(s) 272, 565, 585, 904, 934
BstMAI GTCTC 1 cut(s) 49
BstMBI GATC 5 cut(s) 269, 562, 582, 901, 931
BstMWI GCNNNNNNNGC 2 cut(s) 871, 973
BstNI CCWGG 6 cut(s) 66, 124, 179, 419, 458, 735
BstSCI CCNGG 9 cut(s) 64, 122, 137, 177, 417, 456, 733, 865, 866
BstUI CGCG 2 cut(s) 378, 841
BstV1I GCAGC 3 cut(s) 120, 133, 621
BstV2I GAAGAC 1 cut(s) 408
BstX2I RGATCY 2 cut(s) 269, 562
BstYI RGATCY 2 cut(s) 269, 562
BsuRI GGCC 2 cut(s) 452, 773
BtsCI GGATG 2 cut(s) 453, 876
BtsI GCAGTG 1 cut(s) 409
BtsIMutI CAGTG 2 cut(s) 409, 654
BveI ACCTGC 1 cut(s) 118
Cac8I GCNNGC 2 cut(s) 113, 144
Cfr13I GGNCC 2 cut(s) 450, 737
Cfr9I CCCGGG 1 cut(s) 866
Csp6I GTAC 1 cut(s) 757
CviAII CATG 2 cut(s) 310, 479
CviQI GTAC 1 cut(s) 757
DdeI CTNAG 2 cut(s) 630, 741
DpnI GATC 5 cut(s) 271, 564, 584, 903, 933
DpnII GATC 5 cut(s) 269, 562, 582, 901, 931
DraI TTTAAA 1 cut(s) 985
EaeI YGGCCR 1 cut(s) 771
Eam1104I CTCTTC 2 cut(s) 30, 258
EarI CTCTTC 2 cut(s) 30, 258
Eco24I GRGCYC 1 cut(s) 488
Eco47I GGWCC 1 cut(s) 737
Eco57I CTGAAG 1 cut(s) 932
Eco88I CYCGRG 3 cut(s) 718, 790, 866
EcoRII CCWGG 6 cut(s) 64, 122, 177, 417, 456, 733
EcoT38I GRGCYC 1 cut(s) 488
FaeI CATG 2 cut(s) 313, 482
FatI CATG 2 cut(s) 309, 478
FauNDI CATATG 1 cut(s) 640
FbaI TGATCA 1 cut(s) 582
Fnu4HI GCNGC 3 cut(s) 109, 147, 635
FokI GGATG 2 cut(s) 440, 883
FriOI GRGCYC 1 cut(s) 488
Fsp4HI GCNGC 3 cut(s) 109, 147, 635
FspBI CTAG 2 cut(s) 566, 905
GluI GCNGC 3 cut(s) 109, 147, 635
GsaI CCCAGC 1 cut(s) 390
HaeIII GGCC 2 cut(s) 452, 773
HapII CCGG 2 cut(s) 138, 867
Hin1II CATG 2 cut(s) 313, 482
HindIII AAGCTT 1 cut(s) 965
HpaII CCGG 2 cut(s) 138, 867
Hpy188I TCNGA 5 cut(s) 120, 238, 274, 535, 782
Hpy188III TCNNGA 8 cut(s) 345, 398, 566, 684, 720, 740, 899, 905
HpyAV CCTTC 5 cut(s) 235, 272, 342, 478, 611
HpyCH4III ACNGT 1 cut(s) 821
HpyCH4IV ACGT 2 cut(s) 152, 614
HpyCH4V TGCA 6 cut(s) 149, 188, 227, 414, 851, 874
HpyF10VI GCNNNNNNNGC 2 cut(s) 871, 973
HpyF3I CTNAG 2 cut(s) 630, 741
HpySE526I ACGT 2 cut(s) 152, 614
Hsp92II CATG 2 cut(s) 313, 482
Ksp22I TGATCA 1 cut(s) 582
Kzo9I GATC 5 cut(s) 269, 562, 582, 901, 931
LmnI GCTCC 4 cut(s) 18, 94, 483, 686
Lsp1109I GCAGC 3 cut(s) 120, 133, 621
LweI GCATC 2 cut(s) 852, 861
MaeI CTAG 2 cut(s) 566, 905
MaeII ACGT 2 cut(s) 152, 614
MaeIII GTNAC 1 cut(s) 596
MalI GATC 5 cut(s) 271, 564, 584, 903, 933
MbiI CCGCTC 1 cut(s) 259
MboI GATC 5 cut(s) 269, 562, 582, 901, 931
MboII GAAGA 5 cut(s) 17, 245, 413, 551, 925
MflI RGATCY 2 cut(s) 269, 562
MhlI GDGCHC 2 cut(s) 15, 488
MlsI TGGCCA 1 cut(s) 773
MluCI AATT 3 cut(s) 667, 893, 926
MluNI TGGCCA 1 cut(s) 773
MmeI TCCRAC 1 cut(s) 635
MnlI CCTC 6 cut(s) 39, 42, 45, 126, 282, 299
Mox20I TGGCCA 1 cut(s) 773
MscI TGGCCA 1 cut(s) 773
MseI TTAA 3 cut(s) 372, 884, 984
Msp20I TGGCCA 1 cut(s) 773
MspI CCGG 2 cut(s) 138, 867
MspR9I CCNGG 9 cut(s) 66, 124, 139, 179, 419, 458, 735, 867, 868
MvaI CCWGG 6 cut(s) 66, 124, 179, 419, 458, 735
MvnI CGCG 2 cut(s) 378, 841
MwoI GCNNNNNNNGC 2 cut(s) 871, 973
NciI CCSGG 3 cut(s) 139, 867, 868
NdeI CATATG 1 cut(s) 640
NdeII GATC 5 cut(s) 269, 562, 582, 901, 931
NlaIII CATG 2 cut(s) 313, 482
NlaIV GGNNCC 2 cut(s) 271, 485
PaeR7I CTCGAG 1 cut(s) 718
PasI CCCWGGG 1 cut(s) 418
PkrI GCNGC 3 cut(s) 110, 148, 636
Psp1406I AACGTT 2 cut(s) 152, 614
Psp6I CCWGG 6 cut(s) 64, 122, 177, 417, 456, 733
PspFI CCCAGC 1 cut(s) 386
PspGI CCWGG 6 cut(s) 64, 122, 177, 417, 456, 733
PspN4I GGNNCC 2 cut(s) 271, 485
PspPI GGNCC 2 cut(s) 450, 737
PsuI RGATCY 2 cut(s) 269, 562
RsaI GTAC 1 cut(s) 758
RsaNI GTAC 1 cut(s) 757
SaqAI TTAA 3 cut(s) 372, 884, 984
SatI GCNGC 3 cut(s) 109, 147, 635
Sau3AI GATC 5 cut(s) 269, 562, 582, 901, 931
Sau96I GGNCC 2 cut(s) 450, 737
ScrFI CCNGG 9 cut(s) 66, 124, 139, 179, 419, 458, 735, 867, 868
SduI GDGCHC 2 cut(s) 15, 488
SfaNI GCATC 2 cut(s) 852, 861
Sfr274I CTCGAG 1 cut(s) 718
SinI GGWCC 1 cut(s) 737
SlaI CTCGAG 1 cut(s) 718
SmaI CCCGGG 1 cut(s) 868
SmlI CTYRAG 1 cut(s) 718
SmoI CTYRAG 1 cut(s) 718
Sse9I AATT 3 cut(s) 667, 893, 926
SsiI CCGC 3 cut(s) 257, 378, 841
SspMI CTAG 2 cut(s) 566, 905
StyD4I CCNGG 9 cut(s) 64, 122, 137, 177, 417, 456, 733, 865, 866
TaaI ACNGT 1 cut(s) 821
TaiI ACGT 2 cut(s) 155, 617
TaqI TCGA 4 cut(s) 261, 719, 860, 951
TasI AATT 3 cut(s) 667, 893, 926
TatI WGTACW 1 cut(s) 756
Tru1I TTAA 3 cut(s) 372, 884, 984
Tru9I TTAA 3 cut(s) 372, 884, 984
TscAI CASTG 2 cut(s) 416, 661
TseI GCWGC 3 cut(s) 108, 146, 634
TspDTI ATGAA 4 cut(s) 151, 291, 467, 736
TspGWI ACGGA 1 cut(s) 575
TspMI CCCGGG 1 cut(s) 866
TspRI CASTG 2 cut(s) 416, 661
VpaK11BI GGWCC 1 cut(s) 737
XbaI TCTAGA 2 cut(s) 565, 904
XhoI CTCGAG 1 cut(s) 718
XmaI CCCGGG 1 cut(s) 866
XspI CTAG 2 cut(s) 566, 905
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.