Rh5DG270300
ERF Family

Belongs to the calycin superfamily. Lipocalin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0009620
Physical Location & Seq
Forward (+)
32912371 .. 32915325
2955 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG270300.1

Sequence Viewer

Length: 1002 bp
ATGGTTCATGCTCTTTTACAAACAGCACCACTTCTCCTCCAGTGTTCTCCTCATGTCTCTTCCTGTAGGGGAAAGCCTGGGAAAATAGTGATGAAGTGCTGTGCAGAGCAATCTATGAGCAACAAGCCAGTGACCAAACATGTATTATCTGGGTTTGCGGCATCTTTAGTACTTCTCTCTCAAATAAACCAGGCTGTTGCATCAGAAGTTTACTTTCAAAGAAATACTTATGAGCTTGCAAATGCTGCTGAGAAGACAGTGACTCTTCCTCTTGATAAGGGTTCTGATGAAGGAGCTGAGAGGCTAATGATGATGAGAGGCATGACAGCAAACAATTTTGACCCCATTAGATATTCTGGTAGGTGGTATGAAGTTGCTTCACTTAAACGTGGATTTGCTGGGCAAGGTCAGGAAGACTGTCATTGCACCCAGGGTGTATATACCTTTGATATTGAGAAGAGGGCCATCCAGGTTGATACCTTCTGTGTTCATGGGTCTCCTGAAGGTTATATAACTGGCATAAGGGGAAACGTTCAATGCCTTTCAGATAAAGATTTGGAAAAGAATGAGTCAGATCTAGAAATGCAGGAGATGATCAAAGAGAAGTGTTTCCTCCGTTTTCCAACATTGCCATTTATCCCTAAGTTGCCGTATGATGTGATTGCAACTGATTATGACAATTTTGCTCTTGTTTCAGGAGCAAAAGATACAGGTTTCATACAGATCTACTCCAGAACACCAACTCCTGGTCCTGAATTCATAGAGAAGTACAAATCATACTTGGCCAACTTTGGATATGACCCAACCAAAATCAAGGACACCCCACAAGACTGTGAAGTGATGTCGGACAGTCGGCTAGCTGCAATGATGTCAATGTCCGGAATGCAAAAATTTTTAACAAATGAGTTTCCTGATCTAGAATTGAAGCGCCCTGTTCAATTCGACCCCTTCACTAGTGTATTTGACACTCTGAAGAAGCTTGTTCAGCTTTATTTCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000302 GO:0001101 GO:0001817 GO:0001818 GO:0001952 GO:0001953 GO:0002682 GO:0002683 GO:0002685 GO:0002686 GO:0003006 GO:0003674 GO:0005488 GO:0005496 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005829 GO:0005840 GO:0005975 GO:0005996 GO:0006006 GO:0006629 GO:0006950 GO:0006979 GO:0007162 GO:0007275 GO:0007399 GO:0007417 GO:0007420 GO:0007568 GO:0008150 GO:0008152 GO:0008285 GO:0008289 GO:0009266 GO:0009314 GO:0009408 GO:0009414 GO:0009415 GO:0009416 GO:0009507 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0009611 GO:0009628 GO:0009635 GO:0009636 GO:0009642 GO:0009644 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009888 GO:0009892 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010117 GO:0010154 GO:0010431 GO:0010565 GO:0010605 GO:0010640 GO:0010642 GO:0010646 GO:0010648 GO:0010810 GO:0010812 GO:0012505 GO:0014012 GO:0015485 GO:0016020 GO:0016043 GO:0019216 GO:0019217 GO:0019222 GO:0019318 GO:0021700 GO:0022008 GO:0022414 GO:0022626 GO:0023051 GO:0023057 GO:0030030 GO:0030154 GO:0030155 GO:0030182 GO:0030334 GO:0030336 GO:0030425 GO:0031099 GO:0031102 GO:0031103 GO:0031175 GO:0031323 GO:0031324 GO:0031347 GO:0031348 GO:0031976 GO:0031977 GO:0031984 GO:0032101 GO:0032102 GO:0032386 GO:0032387 GO:0032501 GO:0032502 GO:0032504 GO:0032642 GO:0032682 GO:0032879 GO:0032880 GO:0032934 GO:0032991 GO:0033157 GO:0033554 GO:0033993 GO:0034357 GO:0034442 GO:0034443 GO:0036094 GO:0036477 GO:0040007 GO:0040012 GO:0040013 GO:0042060 GO:0042127 GO:0042221 GO:0042246 GO:0042306 GO:0042308 GO:0042493 GO:0042651 GO:0042995 GO:0043005 GO:0043025 GO:0043178 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044087 GO:0044238 GO:0044281 GO:0044297 GO:0044421 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044445 GO:0044446 GO:0044463 GO:0044464 GO:0045833 GO:0045922 GO:0046320 GO:0046322 GO:0046822 GO:0046823 GO:0048316 GO:0048468 GO:0048471 GO:0048513 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048589 GO:0048608 GO:0048609 GO:0048660 GO:0048662 GO:0048666 GO:0048678 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050727 GO:0050728 GO:0050746 GO:0050748 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051051 GO:0051128 GO:0051129 GO:0051171 GO:0051172 GO:0051223 GO:0051224 GO:0051239 GO:0051241 GO:0051246 GO:0051248 GO:0051270 GO:0051271 GO:0051716 GO:0051893 GO:0051895 GO:0055035 GO:0060255 GO:0060322 GO:0060341 GO:0060587 GO:0060588 GO:0061458 GO:0061564 GO:0062012 GO:0062014 GO:0065007 GO:0070201 GO:0071637 GO:0071638 GO:0071695 GO:0071704 GO:0071840 GO:0080090 GO:0080134 GO:0090087 GO:0090109 GO:0090317 GO:0097159 GO:0097305 GO:0097447 GO:0097458 GO:0120025 GO:0120036 GO:0120038 GO:1900015 GO:1900016 GO:1900180 GO:1900181 GO:1901562 GO:1901700 GO:1901888 GO:1901889 GO:1903391 GO:1903392 GO:1903827 GO:1903828 GO:1904589 GO:1904590 GO:1904950 GO:1990904 GO:2000097 GO:2000098 GO:2000145 GO:2000146 GO:2000401 GO:2000402 GO:2000404 GO:2000405
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipocalin_2

Protein Analysis

333

Amino Acids

37.45

Weight (kDa)

6.02

Isoelectric Point (pI)

35.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipocalin_2 PF08212 116 - 276 1.9e-09 Lipocalin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 746
AccIII TCCGGA 1 cut(s) 878
AciI CCGC 1 cut(s) 158
AclI AACGTT 1 cut(s) 531
AcoI YGGCCR 1 cut(s) 783
AcsI RAATTY 2 cut(s) 755, 890
AcuI CTGAAG 2 cut(s) 522, 992
AfaI GTAC 2 cut(s) 171, 770
AfiI CCNNNNNNNGG 1 cut(s) 746
AflIII ACRYGT 1 cut(s) 139
AgsI TTSAA 5 cut(s) 218, 536, 925, 938, 997
AhlI ACTAGT 1 cut(s) 953
AjnI CCWGG 5 cut(s) 76, 189, 429, 468, 745
AloI GAACNNNNNNTCC 2 cut(s) 727, 759
AluBI AGCT 5 cut(s) 235, 296, 860, 979, 988
AluI AGCT 5 cut(s) 235, 296, 860, 979, 988
Alw26I GTCTC 2 cut(s) 61, 501
Aor13HI TCCGGA 1 cut(s) 878
AoxI GGCC 2 cut(s) 462, 783
ApeKI GCWGC 2 cut(s) 245, 860
ApoI RAATTY 2 cut(s) 755, 890
Asp700I GAANNNNTTC 1 cut(s) 608
AspLEI GCGC 1 cut(s) 930
AspS9I GGNCC 2 cut(s) 462, 749
AsuNHI GCTAGC 1 cut(s) 856
AvaII GGWCC 1 cut(s) 749
BalI TGGCCA 1 cut(s) 785
BbsI GAAGAC 2 cut(s) 260, 420
BbvI GCAGC 2 cut(s) 232, 847
BccI CCATC 1 cut(s) 473
BceAI ACGGC 1 cut(s) 634
BciT130I CCWGG 5 cut(s) 78, 191, 431, 470, 747
BclI TGATCA 1 cut(s) 594
BcoDI GTCTC 2 cut(s) 61, 501
BcuI ACTAGT 1 cut(s) 953
BfaI CTAG 4 cut(s) 578, 857, 917, 954
BfmI CTRYAG 1 cut(s) 64
BfoI RGCGCY 1 cut(s) 931
BglII AGATCT 2 cut(s) 574, 723
BisI GCNGC 3 cut(s) 159, 246, 861
BlsI GCNGC 3 cut(s) 160, 247, 862
BmcAI AGTACT 1 cut(s) 171
Bme1390I CCNGG 5 cut(s) 78, 191, 431, 470, 747
Bme18I GGWCC 1 cut(s) 749
BmgT120I GGNCC 2 cut(s) 462, 749
BmrFI CCNGG 5 cut(s) 78, 191, 431, 470, 747
BmsI GCATC 2 cut(s) 170, 209
BmtI GCTAGC 1 cut(s) 860
BpiI GAAGAC 2 cut(s) 260, 420
BpmI CTGGAG 2 cut(s) 23, 715
BsaI GGTCTC 1 cut(s) 501
BsaJI CCNNGG 3 cut(s) 77, 429, 430
BsaWI WCCGGW 1 cut(s) 878
BsaXI ACNNNNNCTCC 6 cut(s) 18, 21, 48, 51, 727, 757
Bsc4I CCNNNNNNNGG 1 cut(s) 746
Bse1I ACTGG 3 cut(s) 40, 128, 520
Bse3DI GCAATG 3 cut(s) 421, 626, 870
BseAI TCCGGA 1 cut(s) 878
BseBI CCWGG 5 cut(s) 78, 191, 431, 470, 747
BseDI CCNNGG 3 cut(s) 77, 429, 430
BseGI GGATG 1 cut(s) 465
BseLI CCNNNNNNNGG 1 cut(s) 746
BseMI GCAATG 3 cut(s) 421, 626, 870
BseMII CTCAG 2 cut(s) 240, 288
BseNI ACTGG 3 cut(s) 40, 128, 520
BseRI GAGGAG 2 cut(s) 26, 39
BseXI GCAGC 2 cut(s) 232, 847
BseYI CCCAGC 1 cut(s) 398
BsgI GTGCAG 1 cut(s) 123
BshFI GGCC 2 cut(s) 464, 785
BsiSI CCGG 1 cut(s) 879
BslI CCNNNNNNNGG 1 cut(s) 746
BsmAI GTCTC 2 cut(s) 61, 501
BsmI GAATGC 1 cut(s) 888
BsnI GGCC 2 cut(s) 464, 785
Bso31I GGTCTC 1 cut(s) 501
Bsp13I TCCGGA 1 cut(s) 878
Bsp143I GATC 4 cut(s) 574, 594, 723, 913
BspACI CCGC 1 cut(s) 158
BspANI GGCC 2 cut(s) 464, 785
BspCNI CTCAG 2 cut(s) 241, 289
BspEI TCCGGA 1 cut(s) 878
BspOI GCTAGC 1 cut(s) 860
BspTNI GGTCTC 1 cut(s) 501
BsrDI GCAATG 3 cut(s) 421, 626, 870
BsrI ACTGG 3 cut(s) 40, 128, 520
BssECI CCNNGG 3 cut(s) 77, 429, 430
BssMI GATC 4 cut(s) 574, 594, 723, 913
Bst2UI CCWGG 5 cut(s) 78, 191, 431, 470, 747
Bst4CI ACNGT 4 cut(s) 259, 419, 833, 851
Bst6I CTCTTC 3 cut(s) 64, 270, 452
BstAPI GCANNNNNTGC 1 cut(s) 245
BstC8I GCNNGC 2 cut(s) 237, 858
BstDEI CTNAG 3 cut(s) 249, 297, 642
BstF5I GGATG 1 cut(s) 465
BstH2I RGCGCY 1 cut(s) 931
BstHHI GCGC 1 cut(s) 930
BstKTI GATC 4 cut(s) 577, 597, 726, 916
BstMAI GTCTC 2 cut(s) 61, 501
BstMBI GATC 4 cut(s) 574, 594, 723, 913
BstMWI GCNNNNNNNGC 2 cut(s) 245, 985
BstNI CCWGG 5 cut(s) 78, 191, 431, 470, 747
BstNSI RCATGY 1 cut(s) 143
BstSCI CCNGG 5 cut(s) 76, 189, 429, 468, 745
BstSFI CTRYAG 1 cut(s) 64
BstV1I GCAGC 2 cut(s) 232, 847
BstV2I GAAGAC 2 cut(s) 260, 420
BstX2I RGATCY 2 cut(s) 574, 723
BstYI RGATCY 2 cut(s) 574, 723
BsuRI GGCC 2 cut(s) 464, 785
BtsCI GGATG 1 cut(s) 465
BtsIMutI CAGTG 3 cut(s) 47, 135, 264
Cac8I GCNNGC 2 cut(s) 237, 858
CfoI GCGC 1 cut(s) 930
Cfr13I GGNCC 2 cut(s) 462, 749
Csp6I GTAC 2 cut(s) 170, 769
CviAII CATG 5 cut(s) 8, 53, 140, 322, 491
CviQI GTAC 2 cut(s) 170, 769
DdeI CTNAG 3 cut(s) 249, 297, 642
DpnI GATC 4 cut(s) 576, 596, 725, 915
DpnII GATC 4 cut(s) 574, 594, 723, 913
EaeI YGGCCR 1 cut(s) 783
Eam1104I CTCTTC 3 cut(s) 64, 270, 452
EarI CTCTTC 3 cut(s) 64, 270, 452
Eco31I GGTCTC 1 cut(s) 501
Eco47I GGWCC 1 cut(s) 749
Eco57I CTGAAG 2 cut(s) 522, 992
EcoRI GAATTC 1 cut(s) 755
EcoRII CCWGG 5 cut(s) 76, 189, 429, 468, 745
FaeI CATG 5 cut(s) 11, 56, 143, 325, 494
FalI AAGNNNNNCTT 2 cut(s) 211, 243
FatI CATG 5 cut(s) 7, 52, 139, 321, 490
FbaI TGATCA 1 cut(s) 594
Fnu4HI GCNGC 3 cut(s) 159, 246, 861
FokI GGATG 1 cut(s) 452
Fsp4HI GCNGC 3 cut(s) 159, 246, 861
FspBI CTAG 4 cut(s) 578, 857, 917, 954
GlaI GCGC 1 cut(s) 929
GluI GCNGC 3 cut(s) 159, 246, 861
GsaI CCCAGC 1 cut(s) 402
GsuI CTGGAG 2 cut(s) 23, 715
HaeII RGCGCY 1 cut(s) 931
HaeIII GGCC 2 cut(s) 464, 785
HapII CCGG 1 cut(s) 879
HhaI GCGC 1 cut(s) 930
Hin1II CATG 5 cut(s) 11, 56, 143, 325, 494
Hin6I GCGC 1 cut(s) 928
HinP1I GCGC 1 cut(s) 928
HindIII AAGCTT 1 cut(s) 977
HinfI GANTC 2 cut(s) 262, 569
HpaII CCGG 1 cut(s) 879
Hpy166II GTNNAC 1 cut(s) 211
Hpy188I TCNGA 6 cut(s) 205, 286, 547, 574, 847, 972
Hpy8I GTNNAC 1 cut(s) 211
HpyAV CCTTC 4 cut(s) 284, 490, 497, 958
HpyCH4III ACNGT 4 cut(s) 259, 419, 833, 851
HpyCH4IV ACGT 2 cut(s) 388, 531
HpyCH4V TGCA 8 cut(s) 104, 200, 239, 426, 586, 665, 863, 886
HpyF10VI GCNNNNNNNGC 2 cut(s) 245, 985
HpyF3I CTNAG 3 cut(s) 249, 297, 642
HpySE526I ACGT 2 cut(s) 388, 531
Hsp92II CATG 5 cut(s) 11, 56, 143, 325, 494
HspAI GCGC 1 cut(s) 928
Kpn2I TCCGGA 1 cut(s) 878
Ksp22I TGATCA 1 cut(s) 594
Kzo9I GATC 4 cut(s) 574, 594, 723, 913
LmnI GCTCC 2 cut(s) 293, 698
Lsp1109I GCAGC 2 cut(s) 232, 847
LweI GCATC 2 cut(s) 170, 209
MaeI CTAG 4 cut(s) 578, 857, 917, 954
MaeII ACGT 2 cut(s) 388, 531
MaeIII GTNAC 2 cut(s) 130, 259
MalI GATC 4 cut(s) 576, 596, 725, 915
MboI GATC 4 cut(s) 574, 594, 723, 913
MboII GAAGA 6 cut(s) 51, 257, 265, 425, 469, 985
MflI RGATCY 2 cut(s) 574, 723
MlsI TGGCCA 1 cut(s) 785
MluCI AATT 6 cut(s) 334, 679, 755, 890, 920, 938
MluNI TGGCCA 1 cut(s) 785
MlyI GAGTC 2 cut(s) 256, 578
MmeI TCCRAC 2 cut(s) 647, 825
MnlI CCTC 7 cut(s) 47, 60, 279, 294, 311, 453, 623
Mox20I TGGCCA 1 cut(s) 785
MroI TCCGGA 1 cut(s) 878
MroXI GAANNNNTTC 1 cut(s) 608
MscI TGGCCA 1 cut(s) 785
MseI TTAA 2 cut(s) 384, 896
Msp20I TGGCCA 1 cut(s) 785
MspI CCGG 1 cut(s) 879
MspR9I CCNGG 5 cut(s) 78, 191, 431, 470, 747
Mva1269I GAATGC 1 cut(s) 888
MvaI CCWGG 5 cut(s) 78, 191, 431, 470, 747
MwoI GCNNNNNNNGC 2 cut(s) 245, 985
NdeII GATC 4 cut(s) 574, 594, 723, 913
NheI GCTAGC 1 cut(s) 856
NlaIII CATG 5 cut(s) 11, 56, 143, 325, 494
NmuCI GTSAC 2 cut(s) 130, 259
NspI RCATGY 1 cut(s) 143
PasI CCCWGGG 1 cut(s) 430
PciI ACATGT 1 cut(s) 139
PctI GAATGC 1 cut(s) 888
PdmI GAANNNNTTC 1 cut(s) 608
PflMI CCANNNNNTGG 1 cut(s) 746
PkrI GCNGC 3 cut(s) 160, 247, 862
PleI GAGTC 2 cut(s) 256, 577
PpsI GAGTC 2 cut(s) 256, 577
PscI ACATGT 1 cut(s) 139
Psp1406I AACGTT 1 cut(s) 531
Psp6I CCWGG 5 cut(s) 76, 189, 429, 468, 745
PspFI CCCAGC 1 cut(s) 398
PspGI CCWGG 5 cut(s) 76, 189, 429, 468, 745
PspPI GGNCC 2 cut(s) 462, 749
PsuI RGATCY 2 cut(s) 574, 723
RsaI GTAC 2 cut(s) 171, 770
RsaNI GTAC 2 cut(s) 170, 769
SaqAI TTAA 2 cut(s) 384, 896
SatI GCNGC 3 cut(s) 159, 246, 861
Sau3AI GATC 4 cut(s) 574, 594, 723, 913
Sau96I GGNCC 2 cut(s) 462, 749
ScaI AGTACT 1 cut(s) 171
SchI GAGTC 2 cut(s) 256, 578
ScrFI CCNGG 5 cut(s) 78, 191, 431, 470, 747
SfaNI GCATC 2 cut(s) 170, 209
SfcI CTRYAG 1 cut(s) 64
SinI GGWCC 1 cut(s) 749
SpeI ACTAGT 1 cut(s) 953
Sse9I AATT 6 cut(s) 334, 679, 755, 890, 920, 938
SsiI CCGC 1 cut(s) 158
SspMI CTAG 4 cut(s) 578, 857, 917, 954
StyD4I CCNGG 5 cut(s) 76, 189, 429, 468, 745
TaaI ACNGT 4 cut(s) 259, 419, 833, 851
TaiI ACGT 2 cut(s) 391, 534
TaqI TCGA 1 cut(s) 942
TasI AATT 6 cut(s) 334, 679, 755, 890, 920, 938
TatI WGTACW 2 cut(s) 169, 768
TauI GCSGC 1 cut(s) 161
Tru1I TTAA 2 cut(s) 384, 896
Tru9I TTAA 2 cut(s) 384, 896
TscAI CASTG 3 cut(s) 47, 135, 264
TseFI GTSAC 2 cut(s) 130, 259
TseI GCWGC 2 cut(s) 245, 860
Tsp45I GTSAC 2 cut(s) 130, 259
TspDTI ATGAA 6 cut(s) 107, 303, 384, 479, 706, 748
TspGWI ACGGA 1 cut(s) 605
TspRI CASTG 3 cut(s) 47, 135, 264
Van91I CCANNNNNTGG 1 cut(s) 746
VpaK11BI GGWCC 1 cut(s) 749
XapI RAATTY 2 cut(s) 755, 890
XbaI TCTAGA 2 cut(s) 577, 916
XceI RCATGY 1 cut(s) 143
XmnI GAANNNNTTC 1 cut(s) 608
XspI CTAG 4 cut(s) 578, 857, 917, 954
ZrmI AGTACT 1 cut(s) 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.