MD11G1265700.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0010007
Physical Location & Seq
Forward (+)
38086165 .. 38090364
4200 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1265700.v1.1.491

Sequence Viewer

Length: 1245 bp
ATGAGAAATTCCCATCTCTTCCTCACTCTCCTAACACTCACAGTCACTTACTCCCTCCGATTCCAAGTTCATGCTGCTCCTGCAGGGCCATTGATAAAGCACTTGTCTTCTCTTCTCAAGTGGACTGGGGAAATATCCTCCAAAACACCCCAATCAGAAAAGAATGTTCTTCAATTTGAGAATGGGTACTTAGTTGAGACTGTTGTGGAAGGAAATGACATTGGTGTTATTCCTCACAAAATCCGCATCTCAGAGGATGGGGAGCTCTTTGCTGTGGACTCCGTTAATAGCAACATTATGCGCATTGCTTCACCCTTGTCCCAATATAGTAGGGGAAGATTGGTTGCTGGGTCATTTCAGGGTTATGCTGGACATGTTGATGGGAAGCCAAGTGATGCTCGTTTCAATCATCCCAAAGGTGTAACCATTGATGATAAGGGGAATGTGTATGTTGCTGATACCTTGAATCACGCCATACGAAAGATTGGAGATGCTGGAGTAACAACCATTGCAGGTGGGAAATCAAATGTTGCAGGCTATAGAGATGGGCCCAGTGAGGATGCCATGTTCTCAAATGATTTCGATATCGTATATGTTCAGCCTACTTGTTCGTTGCTAGTTGTTGATAGAGGAAATGCTGCTCTCCGGCAGATCTCTCTCAATCAGGAGGATTGTGATTATCAGTACAGTTCAATTTCTGCCACAGATATCCTTATGGTTGTTGGTGCTGTCTTGGTAGGGTATACTACATGCATACTGCAGCAGGGATATGGACCTTCTTTCTTATTGAGAACGCAGCCACTTTCAAAGAGTGAAATGAAAGAACATCCAAGCAATGGGAAATCCACGCTACTTGTGAAAAGCATGAAAGAGGAACCGGGATGGCCATCTTTTGGACAGCTTGTCAGTGATCTATCAAAGCTTGGACTTGCAGCATTGGCTGGCATATTCGTTCACTTCCTTCCGTCGCGTTTTATGCCTGGTGGCTCCCAGAAAGGCCTAACACCATTAAAAGATTCTCTTAGGATGCCTGAAGATGAAGCCGAGGCTCCAGTAGTTCAGAGACAAAGTGCTCCTGCTCCTCTGTCTGAAACTCGACAGGCCTATACCCCCAATGCAAGTGAGAAGTATTCAGATATGAAGCCAGCAAAAATGAAGTCAACTAGTTTCAAAGATCCCTCTTTGTCAAGCAAGCACCGGTCTTCAAAACGACAGAACTCTGCAGAATTCTATGGATCCTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NHL

Protein Analysis

415

Amino Acids

45.1

Weight (kDa)

8.24

Isoelectric Point (pI)

36.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NHL PF01436 135 - 161 3.8e-06 NHL repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 503
Acc16I TGCGCA 1 cut(s) 302
Acc36I ACCTGC 1 cut(s) 503
AccB7I CCANNNNNTGG 2 cut(s) 836, 893
AccI GTMKAC 1 cut(s) 743
AccII CGCG 1 cut(s) 970
AciI CCGC 1 cut(s) 244
AclWI GGATC 3 cut(s) 1169, 1230, 1243
AcoI YGGCCR 1 cut(s) 884
AcsI RAATTY 2 cut(s) 7, 1226
AcuI CTGAAG 1 cut(s) 1053
AfaI GTAC 2 cut(s) 188, 686
AfiI CCNNNNNNNGG 2 cut(s) 836, 893
AflIII ACRYGT 1 cut(s) 373
AgeI ACCGGT 1 cut(s) 1197
AgsI TTSAA 7 cut(s) 173, 406, 466, 693, 807, 1171, 1206
AhdI GACNNNNNGTC 1 cut(s) 902
AhlI ACTAGT 1 cut(s) 1163
AjnI CCWGG 2 cut(s) 979, 1238
AloI GAACNNNNNNTCC 2 cut(s) 551, 583
AluBI AGCT 3 cut(s) 265, 901, 922
AluI AGCT 3 cut(s) 265, 901, 922
Alw21I GWGCWC 2 cut(s) 267, 1075
Alw26I GTCTC 2 cut(s) 191, 1057
AlwI GGATC 3 cut(s) 1169, 1230, 1243
AoxI GGCC 5 cut(s) 86, 548, 884, 997, 1101
ApaI GGGCCC 1 cut(s) 552
ApeKI GCWGC 5 cut(s) 74, 638, 760, 796, 932
ApoI RAATTY 2 cut(s) 7, 1226
ArsI GACNNNNNNTTYG 2 cut(s) 1142, 1174
AsiGI ACCGGT 1 cut(s) 1197
AspLEI GCGC 1 cut(s) 303
AspS9I GGNCC 4 cut(s) 86, 548, 549, 773
AsuC2I CCSGG 1 cut(s) 879
AsuHPI GGTGA 1 cut(s) 303
AvaII GGWCC 1 cut(s) 773
BaeGI GKGCMC 1 cut(s) 552
BalI TGGCCA 1 cut(s) 886
BamHI GGATCC 1 cut(s) 1235
BanII GRGCYC 2 cut(s) 267, 552
BbsI GAAGAC 2 cut(s) 99, 1194
Bbv12I GWGCWC 2 cut(s) 267, 1075
BbvI GCAGC 5 cut(s) 61, 625, 772, 808, 944
BccI CCATC 6 cut(s) 21, 251, 374, 539, 876, 895
BciT130I CCWGG 2 cut(s) 981, 1240
BcnI CCSGG 1 cut(s) 879
BcoDI GTCTC 2 cut(s) 191, 1057
BcuI ACTAGT 1 cut(s) 1163
BfaI CTAG 2 cut(s) 617, 1164
BfmI CTRYAG 4 cut(s) 81, 538, 758, 1221
BfuAI ACCTGC 1 cut(s) 503
BglII AGATCT 1 cut(s) 651
BisI GCNGC 5 cut(s) 75, 639, 761, 797, 933
BlsI GCNGC 5 cut(s) 76, 640, 762, 798, 934
Bme1390I CCNGG 3 cut(s) 879, 981, 1240
Bme18I GGWCC 1 cut(s) 773
BmeRI GACNNNNNGTC 1 cut(s) 902
BmgT120I GGNCC 4 cut(s) 86, 548, 549, 773
BmiI GGNNCC 5 cut(s) 550, 876, 988, 1050, 1237
BmrFI CCNGG 3 cut(s) 879, 981, 1240
BmrI ACTGGG 2 cut(s) 135, 546
BmsI GCATC 5 cut(s) 255, 385, 481, 550, 1017
BmuI ACTGGG 2 cut(s) 135, 546
BpiI GAAGAC 2 cut(s) 99, 1194
BpmI CTGGAG 2 cut(s) 516, 1035
BpuEI CTTGAG 1 cut(s) 101
BpuMI CCSGG 1 cut(s) 879
BsaBI GATNNNNATC 1 cut(s) 886
BsaJI CCNNGG 1 cut(s) 1044
BsaWI WCCGGW 1 cut(s) 1197
Bsc4I CCNNNNNNNGG 2 cut(s) 836, 893
Bse118I RCCGGY 1 cut(s) 1197
Bse1I ACTGG 3 cut(s) 130, 552, 1052
Bse3DI GCAATG 3 cut(s) 303, 507, 841
Bse8I GATNNNNATC 1 cut(s) 886
BseBI CCWGG 2 cut(s) 981, 1240
BseDI CCNNGG 1 cut(s) 1044
BseGI GGATG 6 cut(s) 262, 409, 565, 826, 887, 1032
BseJI GATNNNNATC 1 cut(s) 886
BseLI CCNNNNNNNGG 2 cut(s) 836, 893
BseMI GCAATG 3 cut(s) 303, 507, 841
BseMII CTCAG 1 cut(s) 264
BseNI ACTGG 3 cut(s) 130, 552, 1052
BseRI GAGGAG 1 cut(s) 1071
BseSI GKGCMC 1 cut(s) 552
BseXI GCAGC 5 cut(s) 61, 625, 772, 808, 944
BseYI CCCAGC 1 cut(s) 347
Bsh1236I CGCG 1 cut(s) 970
BshFI GGCC 5 cut(s) 88, 550, 886, 999, 1103
BshTI ACCGGT 1 cut(s) 1197
BsiHKAI GWGCWC 2 cut(s) 267, 1075
BsiSI CCGG 3 cut(s) 646, 878, 1198
BslFI GGGAC 1 cut(s) 304
BslI CCNNNNNNNGG 2 cut(s) 836, 893
BsmAI GTCTC 2 cut(s) 191, 1057
BsmFI GGGAC 1 cut(s) 304
BsnI GGCC 5 cut(s) 88, 550, 886, 999, 1103
Bsp120I GGGCCC 1 cut(s) 548
Bsp1286I GDGCHC 3 cut(s) 267, 552, 1075
Bsp143I GATC 4 cut(s) 651, 910, 1174, 1235
BspACI CCGC 1 cut(s) 244
BspANI GGCC 5 cut(s) 88, 550, 886, 999, 1103
BspCNI CTCAG 1 cut(s) 263
BspFNI CGCG 1 cut(s) 970
BspLI GGNNCC 5 cut(s) 550, 876, 988, 1050, 1237
BspMAI CTGCAG 3 cut(s) 85, 762, 1225
BspMI ACCTGC 1 cut(s) 503
BspPI GGATC 3 cut(s) 1169, 1230, 1243
BsrDI GCAATG 3 cut(s) 303, 507, 841
BsrFI RCCGGY 1 cut(s) 1197
BsrI ACTGG 3 cut(s) 130, 552, 1052
BssAI RCCGGY 1 cut(s) 1197
BssECI CCNNGG 1 cut(s) 1044
BssMI GATC 4 cut(s) 651, 910, 1174, 1235
BssNAI GTATAC 1 cut(s) 744
Bst1107I GTATAC 1 cut(s) 744
Bst2UI CCWGG 2 cut(s) 981, 1240
Bst4CI ACNGT 3 cut(s) 43, 202, 689
Bst6I CTCTTC 2 cut(s) 23, 117
BstC8I GCNNGC 4 cut(s) 535, 943, 1146, 1193
BstDEI CTNAG 3 cut(s) 190, 250, 1022
BstF5I GGATG 6 cut(s) 262, 409, 565, 826, 887, 1032
BstFNI CGCG 1 cut(s) 970
BstHHI GCGC 1 cut(s) 303
BstKTI GATC 4 cut(s) 654, 913, 1177, 1238
BstMAI GTCTC 2 cut(s) 191, 1057
BstMBI GATC 4 cut(s) 651, 910, 1174, 1235
BstMWI GCNNNNNNNGC 3 cut(s) 80, 938, 976
BstNI CCWGG 2 cut(s) 981, 1240
BstNSI RCATGY 2 cut(s) 377, 753
BstSCI CCNGG 3 cut(s) 877, 979, 1238
BstSFI CTRYAG 4 cut(s) 81, 538, 758, 1221
BstSLI GKGCMC 1 cut(s) 552
BstUI CGCG 1 cut(s) 970
BstV1I GCAGC 5 cut(s) 61, 625, 772, 808, 944
BstV2I GAAGAC 2 cut(s) 99, 1194
BstX2I RGATCY 3 cut(s) 651, 1174, 1235
BstYI RGATCY 3 cut(s) 651, 1174, 1235
BstZ17I GTATAC 1 cut(s) 744
BsuRI GGCC 5 cut(s) 88, 550, 886, 999, 1103
BtsCI GGATG 6 cut(s) 262, 409, 565, 826, 887, 1032
BtsIMutI CAGTG 2 cut(s) 559, 913
BveI ACCTGC 1 cut(s) 503
Cac8I GCNNGC 4 cut(s) 535, 943, 1146, 1193
CfoI GCGC 1 cut(s) 303
Cfr10I RCCGGY 1 cut(s) 1197
Cfr13I GGNCC 4 cut(s) 86, 548, 549, 773
Csp6I GTAC 2 cut(s) 187, 685
CspAI ACCGGT 1 cut(s) 1197
CspCI CAANNNNNGTGG 2 cut(s) 835, 870
CviAII CATG 5 cut(s) 71, 374, 565, 750, 865
CviQI GTAC 2 cut(s) 187, 685
DdeI CTNAG 3 cut(s) 190, 250, 1022
DpnI GATC 4 cut(s) 653, 912, 1176, 1237
DpnII GATC 4 cut(s) 651, 910, 1174, 1235
DriI GACNNNNNGTC 1 cut(s) 902
EaeI YGGCCR 1 cut(s) 884
Eam1104I CTCTTC 2 cut(s) 23, 117
Eam1105I GACNNNNNGTC 1 cut(s) 902
EarI CTCTTC 2 cut(s) 23, 117
Ecl136II GAGCTC 1 cut(s) 265
Eco147I AGGCCT 2 cut(s) 999, 1103
Eco24I GRGCYC 2 cut(s) 267, 552
Eco32I GATATC 2 cut(s) 586, 709
Eco47I GGWCC 1 cut(s) 773
Eco53kI GAGCTC 1 cut(s) 265
Eco57I CTGAAG 1 cut(s) 1053
EcoICRI GAGCTC 1 cut(s) 265
EcoRI GAATTC 1 cut(s) 1226
EcoRII CCWGG 2 cut(s) 979, 1238
EcoRV GATATC 2 cut(s) 586, 709
EcoT22I ATGCAT 1 cut(s) 755
EcoT38I GRGCYC 2 cut(s) 267, 552
FaeI CATG 5 cut(s) 74, 377, 568, 753, 868
FalI AAGNNNNNCTT 2 cut(s) 1005, 1037
FaqI GGGAC 1 cut(s) 304
FatI CATG 5 cut(s) 70, 373, 564, 749, 864
FblI GTMKAC 1 cut(s) 743
Fnu4HI GCNGC 5 cut(s) 75, 639, 761, 797, 933
FokI GGATG 6 cut(s) 269, 396, 572, 813, 894, 1039
FriOI GRGCYC 2 cut(s) 267, 552
Fsp4HI GCNGC 5 cut(s) 75, 639, 761, 797, 933
FspAI RTGCGCAY 1 cut(s) 302
FspBI CTAG 2 cut(s) 617, 1164
FspI TGCGCA 1 cut(s) 302
GlaI GCGC 1 cut(s) 302
GluI GCNGC 5 cut(s) 75, 639, 761, 797, 933
GsaI CCCAGC 1 cut(s) 351
GsuI CTGGAG 2 cut(s) 516, 1035
HaeIII GGCC 5 cut(s) 88, 550, 886, 999, 1103
HapII CCGG 3 cut(s) 646, 878, 1198
HhaI GCGC 1 cut(s) 303
Hin1II CATG 5 cut(s) 74, 377, 568, 753, 868
Hin6I GCGC 1 cut(s) 301
HinP1I GCGC 1 cut(s) 301
HincII GTYRAC 1 cut(s) 1161
HindII GTYRAC 1 cut(s) 1161
HindIII AAGCTT 1 cut(s) 920
HinfI GANTC 4 cut(s) 60, 278, 466, 1016
HpaII CCGG 3 cut(s) 646, 878, 1198
HphI GGTGA 1 cut(s) 303
Hpy166II GTNNAC 5 cut(s) 123, 277, 744, 955, 1161
Hpy188I TCNGA 6 cut(s) 59, 157, 253, 1062, 1090, 1135
Hpy188III TCNNGA 1 cut(s) 665
Hpy8I GTNNAC 5 cut(s) 123, 277, 744, 955, 1161
Hpy99I CGWCG 1 cut(s) 970
HpyAV CCTTC 3 cut(s) 203, 786, 971
HpyCH4III ACNGT 3 cut(s) 43, 202, 689
HpyCH4V TGCA 8 cut(s) 83, 512, 533, 753, 760, 932, 1118, 1223
HpyF10VI GCNNNNNNNGC 3 cut(s) 80, 938, 976
HpyF3I CTNAG 3 cut(s) 190, 250, 1022
Hsp92II CATG 5 cut(s) 74, 377, 568, 753, 868
HspAI GCGC 1 cut(s) 301
Kzo9I GATC 4 cut(s) 651, 910, 1174, 1235
LmnI GCTCC 6 cut(s) 82, 262, 992, 1054, 1078, 1084
Lsp1109I GCAGC 5 cut(s) 61, 625, 772, 808, 944
LweI GCATC 5 cut(s) 255, 385, 481, 550, 1017
MaeI CTAG 2 cut(s) 617, 1164
MaeIII GTNAC 3 cut(s) 43, 421, 499
MalI GATC 4 cut(s) 653, 912, 1176, 1237
MboI GATC 4 cut(s) 651, 910, 1174, 1235
MboII GAAGA 7 cut(s) 10, 99, 104, 161, 348, 1046, 1194
MflI RGATCY 3 cut(s) 651, 1174, 1235
MhlI GDGCHC 3 cut(s) 267, 552, 1075
MlsI TGGCCA 1 cut(s) 886
MluCI AATT 4 cut(s) 7, 173, 693, 1226
MluNI TGGCCA 1 cut(s) 886
MlyI GAGTC 1 cut(s) 272
Mox20I TGGCCA 1 cut(s) 886
Mph1103I ATGCAT 1 cut(s) 755
MscI TGGCCA 1 cut(s) 886
MseI TTAA 2 cut(s) 285, 1010
MslI CAYNNNNRTG 1 cut(s) 378
Msp20I TGGCCA 1 cut(s) 886
MspI CCGG 3 cut(s) 646, 878, 1198
MspR9I CCNGG 3 cut(s) 879, 981, 1240
MvaI CCWGG 2 cut(s) 981, 1240
MvnI CGCG 1 cut(s) 970
MwoI GCNNNNNNNGC 3 cut(s) 80, 938, 976
NciI CCSGG 1 cut(s) 879
NdeII GATC 4 cut(s) 651, 910, 1174, 1235
NlaIII CATG 5 cut(s) 74, 377, 568, 753, 868
NlaIV GGNNCC 5 cut(s) 550, 876, 988, 1050, 1237
NmeAIII GCCGAG 1 cut(s) 1069
NmuCI GTSAC 1 cut(s) 43
NsbI TGCGCA 1 cut(s) 302
NsiI ATGCAT 1 cut(s) 755
NspI RCATGY 2 cut(s) 377, 753
PaqCI CACCTGC 1 cut(s) 503
PceI AGGCCT 2 cut(s) 999, 1103
PciI ACATGT 1 cut(s) 373
PfeI GAWTC 3 cut(s) 60, 466, 1016
PflMI CCANNNNNTGG 2 cut(s) 836, 893
PinAI ACCGGT 1 cut(s) 1197
PkrI GCNGC 5 cut(s) 76, 640, 762, 798, 934
PleI GAGTC 1 cut(s) 272
PpsI GAGTC 1 cut(s) 272
PscI ACATGT 1 cut(s) 373
Psp124BI GAGCTC 1 cut(s) 267
Psp6I CCWGG 2 cut(s) 979, 1238
PspFI CCCAGC 1 cut(s) 347
PspGI CCWGG 2 cut(s) 979, 1238
PspN4I GGNNCC 5 cut(s) 550, 876, 988, 1050, 1237
PspOMI GGGCCC 1 cut(s) 548
PspPI GGNCC 4 cut(s) 86, 548, 549, 773
PstI CTGCAG 3 cut(s) 85, 762, 1225
PsuI RGATCY 3 cut(s) 651, 1174, 1235
RsaI GTAC 2 cut(s) 188, 686
RsaNI GTAC 2 cut(s) 187, 685
RseI CAYNNNNRTG 1 cut(s) 378
SacI GAGCTC 1 cut(s) 267
SaqAI TTAA 2 cut(s) 285, 1010
SatI GCNGC 5 cut(s) 75, 639, 761, 797, 933
Sau3AI GATC 4 cut(s) 651, 910, 1174, 1235
Sau96I GGNCC 4 cut(s) 86, 548, 549, 773
SbfI CCTGCAGG 1 cut(s) 85
SchI GAGTC 1 cut(s) 272
ScrFI CCNGG 3 cut(s) 879, 981, 1240
SdaI CCTGCAGG 1 cut(s) 85
SduI GDGCHC 3 cut(s) 267, 552, 1075
SetI ASST 7 cut(s) 267, 421, 464, 517, 778, 903, 924
SfaNI GCATC 5 cut(s) 255, 385, 481, 550, 1017
SfcI CTRYAG 4 cut(s) 81, 538, 758, 1221
SinI GGWCC 1 cut(s) 773
SmiMI CAYNNNNRTG 1 cut(s) 378
SmlI CTYRAG 1 cut(s) 116
SmoI CTYRAG 1 cut(s) 116
SpeI ACTAGT 1 cut(s) 1163
Sse8387I CCTGCAGG 1 cut(s) 85
Sse9I AATT 4 cut(s) 7, 173, 693, 1226
SseBI AGGCCT 2 cut(s) 999, 1103
SsiI CCGC 1 cut(s) 244
SspMI CTAG 2 cut(s) 617, 1164
SstI GAGCTC 1 cut(s) 267
StuI AGGCCT 2 cut(s) 999, 1103
StyD4I CCNGG 3 cut(s) 877, 979, 1238
TaaI ACNGT 3 cut(s) 43, 202, 689
TaqI TCGA 2 cut(s) 582, 1096
TasI AATT 4 cut(s) 7, 173, 693, 1226
TatI WGTACW 1 cut(s) 684
TfiI GAWTC 3 cut(s) 60, 466, 1016
Tru1I TTAA 2 cut(s) 285, 1010
Tru9I TTAA 2 cut(s) 285, 1010
TscAI CASTG 2 cut(s) 559, 913
TseFI GTSAC 1 cut(s) 43
TseI GCWGC 5 cut(s) 74, 638, 760, 796, 932
Tsp45I GTSAC 1 cut(s) 43
TspDTI ATGAA 6 cut(s) 59, 833, 881, 1053, 1154, 1169
TspGWI ACGGA 2 cut(s) 271, 954
TspRI CASTG 2 cut(s) 559, 913
Van91I CCANNNNNTGG 2 cut(s) 836, 893
VpaK11BI GGWCC 1 cut(s) 773
XapI RAATTY 2 cut(s) 7, 1226
XceI RCATGY 2 cut(s) 377, 753
XmiI GTMKAC 1 cut(s) 743
XspI CTAG 2 cut(s) 617, 1164
Zsp2I ATGCAT 1 cut(s) 755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.