Rw5G019280

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0010007
Physical Location & Seq
Reverse (-)
25101358 .. 25105658
4301 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G019280.1

Sequence Viewer

Length: 1302 bp
ATGACAAGTTCCCTTTTCTTCATCACCATCGTCACTCTCACAGTCGCTTACACCCTCCAATTCAAAGCTCATGCTGCTCCTGCAGGGCCATTGATAAAGCACTTGTCTTCCCTTGTCAAGTGGACTAGGGCGGCATCCGCCAAAGCACCCCAGTCAGATGGGAATGTTGTTCAGTTTGAGAGTGGGTACTTAGTTGAGACTGTTGTGGAAGGAACTGATATTGGAGTTGTTCCTTTCAAGATCCGCATTTCAGAGGATGGCGAACTCTTTGCTGTGGACTCCGTTAATAGCAACATTGTTCGGATTACTCCTCCGTTGTCCCAATATAGTAGGGGAAGATTGGTTGCTGGATCATTTCAGGGTTATACAGGCCATGTTGATGGAAAGCCAAGTGATGCTCGTTTCAATCATCCAAAAGGTGTAACCATGGATGATAAAGGGAATGTATATGTTGCTGATACCTTGAATCACGCCATAAGAAAAATTGGAGAAGCTGGTGTGACAACCATAGCTGGTGGAAAATCAAATATTGCTGGCTACAGGGATGGACCCAGTGAAGATGCAATGTTCTCAAACGATTTTGATGTAGTATATGTCCAGCCTACTTGTTCCTTGCTTGTTGTTGATAGAGGAAATGCTGCTCTTAGGCAGATATCTCTCAACCAGGAGGATTGTGATTATCAGTACAGTTCAATCTCTTCCACAGATGTCCTTATGGTTGTTGGTGCTGTCTTGATAGGCTATGCTACATGCATGCTTCAGCAGGGATTTGGACCTTCTTTCTTCTCGAGAACGCAACCTTCAGAGAGTGAAACTAAAGAACATGCAAGCAATGAAAAATCCACTCCAGTTGTGGAGATTGTGAAAGAGGACCCAGGATGGCCATCTTTTGGGCAGCTTATAATTGATCTATGCAAGATTGGACTTGAAGCAGTGACGGGGACATTTCTTCAGTTCATTCCCTCTCGGTTCATGCCTGGTGGTTCTCACAAAGGCCTGACACCATTAAAAGATTCTCTCAGGATGCCTGAGGACGAAGCTGAGACCCCGTTAGTTCACAGACAAAGCACTCCTGCTCCTCTGTCTGAAACTCGGCAGTCCCATACTCCCAATGCAAGTGATAGATATTCAGATATGAAGCCCTCAAAGATAAAGTCAAGTAGCTTTAAAGATCCCTCTTTGCCAAGCAAGCACCGTCTTCAAAACGCCAGGATTCTGCAGAGTTCTATGGATCTGGTGAAAGATCTAAGAGCCAGAAAGAAAGATCACGGCATCGCCAACGAGAGAAGAGTGGAGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NHL

Protein Analysis

433

Amino Acids

47.24

Weight (kDa)

6.84

Isoelectric Point (pI)

37.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NHL PF01436 135 - 161 5.4e-06 NHL repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 902
AccB7I CCANNNNNTGG 1 cut(s) 890
AciI CCGC 3 cut(s) 131, 138, 244
AclWI GGATC 4 cut(s) 235, 358, 1166, 1239
AcoI YGGCCR 1 cut(s) 881
AcuI CTGAAG 3 cut(s) 743, 786, 935
AfaI GTAC 2 cut(s) 188, 686
AfiI CCNNNNNNNGG 1 cut(s) 890
AgsI TTSAA 7 cut(s) 64, 238, 406, 466, 693, 929, 1202
AjnI CCWGG 4 cut(s) 663, 874, 976, 1208
AluBI AGCT 6 cut(s) 68, 494, 512, 898, 1040, 1164
AluI AGCT 6 cut(s) 68, 494, 512, 898, 1040, 1164
Alw26I GTCTC 2 cut(s) 191, 1037
AlwI GGATC 4 cut(s) 235, 358, 1166, 1239
Ama87I CYCGRG 1 cut(s) 787
AoxI GGCC 4 cut(s) 86, 370, 881, 994
ApeKI GCWGC 3 cut(s) 74, 638, 895
ArsI GACNNNNNNTTYG 2 cut(s) 1139, 1171
AspS9I GGNCC 4 cut(s) 86, 548, 773, 871
AsuHPI GGTGA 2 cut(s) 16, 1249
AvaI CYCGRG 1 cut(s) 787
AvaII GGWCC 3 cut(s) 548, 773, 871
AxyI CCTNAGG 1 cut(s) 1029
BalI TGGCCA 1 cut(s) 883
BbsI GAAGAC 2 cut(s) 99, 1190
BbvI GCAGC 3 cut(s) 61, 625, 907
BccI CCATC 7 cut(s) 35, 152, 251, 374, 539, 873, 892
BceAI ACGGC 1 cut(s) 1285
BcgI CGANNNNNNTGC 2 cut(s) 251, 285
BciT130I CCWGG 4 cut(s) 665, 876, 978, 1210
BcoDI GTCTC 2 cut(s) 191, 1037
BfaI CTAG 1 cut(s) 126
BfmI CTRYAG 3 cut(s) 81, 538, 1217
BglII AGATCT 1 cut(s) 1243
BisI GCNGC 4 cut(s) 75, 132, 639, 896
BlsI GCNGC 4 cut(s) 76, 133, 640, 897
Bme1390I CCNGG 4 cut(s) 665, 876, 978, 1210
Bme18I GGWCC 3 cut(s) 548, 773, 871
BmeT110I CYCGRG 1 cut(s) 787
BmgT120I GGNCC 4 cut(s) 86, 548, 773, 871
BmiI GGNNCC 2 cut(s) 550, 873
BmrFI CCNGG 4 cut(s) 665, 876, 978, 1210
BmrI ACTGGG 2 cut(s) 145, 546
BmsI GCATC 5 cut(s) 143, 385, 550, 1014, 1281
BmuI ACTGGG 2 cut(s) 145, 546
BpiI GAAGAC 2 cut(s) 99, 1190
BpmI CTGGAG 1 cut(s) 831
BsaBI GATNNNNATC 1 cut(s) 883
BsaI GGTCTC 1 cut(s) 1037
BsaJI CCNNGG 2 cut(s) 426, 874
BsaXI ACNNNNNCTCC 2 cut(s) 1060, 1090
Bsc4I CCNNNNNNNGG 1 cut(s) 890
Bse1I ACTGG 3 cut(s) 151, 552, 848
Bse21I CCTNAGG 1 cut(s) 1029
Bse3DI GCAATG 2 cut(s) 570, 838
Bse8I GATNNNNATC 1 cut(s) 883
BseBI CCWGG 4 cut(s) 665, 876, 978, 1210
BseDI CCNNGG 2 cut(s) 426, 874
BseGI GGATG 7 cut(s) 134, 262, 409, 436, 550, 884, 1029
BseJI GATNNNNATC 1 cut(s) 883
BseLI CCNNNNNNNGG 1 cut(s) 890
BseMI GCAATG 2 cut(s) 570, 838
BseMII CTCAG 3 cut(s) 1020, 1032, 1033
BseNI ACTGG 3 cut(s) 151, 552, 848
BseRI GAGGAG 2 cut(s) 300, 1068
BseXI GCAGC 3 cut(s) 61, 625, 907
BshFI GGCC 4 cut(s) 88, 372, 883, 996
BsiHKCI CYCGRG 1 cut(s) 787
BslFI GGGAC 3 cut(s) 304, 955, 1084
BslI CCNNNNNNNGG 1 cut(s) 890
BsmAI GTCTC 2 cut(s) 191, 1037
BsmFI GGGAC 3 cut(s) 304, 955, 1084
BsnI GGCC 4 cut(s) 88, 372, 883, 996
Bso31I GGTCTC 1 cut(s) 1037
BsoBI CYCGRG 1 cut(s) 787
Bsp143I GATC 7 cut(s) 240, 350, 907, 1171, 1231, 1243, 1264
Bsp19I CCATGG 1 cut(s) 426
BspACI CCGC 3 cut(s) 131, 138, 244
BspANI GGCC 4 cut(s) 88, 372, 883, 996
BspCNI CTCAG 3 cut(s) 1021, 1032, 1033
BspLI GGNNCC 2 cut(s) 550, 873
BspMAI CTGCAG 2 cut(s) 85, 1221
BspPI GGATC 4 cut(s) 235, 358, 1166, 1239
BspTNI GGTCTC 1 cut(s) 1037
BsrDI GCAATG 2 cut(s) 570, 838
BsrI ACTGG 3 cut(s) 151, 552, 848
BssECI CCNNGG 2 cut(s) 426, 874
BssMI GATC 7 cut(s) 240, 350, 907, 1171, 1231, 1243, 1264
BssT1I CCWWGG 1 cut(s) 426
Bst2UI CCWGG 4 cut(s) 665, 876, 978, 1210
Bst4CI ACNGT 4 cut(s) 43, 202, 689, 1196
Bst6I CTCTTC 2 cut(s) 703, 1282
BstC8I GCNNGC 4 cut(s) 535, 755, 829, 1190
BstDEI CTNAG 6 cut(s) 190, 644, 1019, 1029, 1041, 1247
BstDSI CCRYGG 1 cut(s) 426
BstF5I GGATG 7 cut(s) 134, 262, 409, 436, 550, 884, 1029
BstKTI GATC 7 cut(s) 243, 353, 910, 1174, 1234, 1246, 1267
BstMAI GTCTC 2 cut(s) 191, 1037
BstMBI GATC 7 cut(s) 240, 350, 907, 1171, 1231, 1243, 1264
BstMWI GCNNNNNNNGC 4 cut(s) 74, 80, 137, 1189
BstNI CCWGG 4 cut(s) 665, 876, 978, 1210
BstNSI RCATGY 3 cut(s) 753, 757, 827
BstSCI CCNGG 4 cut(s) 663, 874, 976, 1208
BstSFI CTRYAG 3 cut(s) 81, 538, 1217
BstV1I GCAGC 3 cut(s) 61, 625, 907
BstV2I GAAGAC 2 cut(s) 99, 1190
BstX2I RGATCY 4 cut(s) 240, 1171, 1231, 1243
BstXI CCANNNNNNTGG 2 cut(s) 158, 380
BstYI RGATCY 4 cut(s) 240, 1171, 1231, 1243
Bsu36I CCTNAGG 1 cut(s) 1029
BsuRI GGCC 4 cut(s) 88, 372, 883, 996
BtgI CCRYGG 1 cut(s) 426
BtgZI GCGATG 1 cut(s) 1258
BtsCI GGATG 7 cut(s) 134, 262, 409, 436, 550, 884, 1029
BtsI GCAGTG 1 cut(s) 939
BtsIMutI CAGTG 2 cut(s) 559, 939
Cac8I GCNNGC 4 cut(s) 535, 755, 829, 1190
Cfr13I GGNCC 4 cut(s) 86, 548, 773, 871
Csp6I GTAC 2 cut(s) 187, 685
CspCI CAANNNNNGTGG 2 cut(s) 832, 867
CviAII CATG 7 cut(s) 71, 374, 427, 750, 754, 824, 973
CviQI GTAC 2 cut(s) 187, 685
DdeI CTNAG 6 cut(s) 190, 644, 1019, 1029, 1041, 1247
DpnI GATC 7 cut(s) 242, 352, 909, 1173, 1233, 1245, 1266
DpnII GATC 7 cut(s) 240, 350, 907, 1171, 1231, 1243, 1264
DraI TTTAAA 1 cut(s) 1168
EaeI YGGCCR 1 cut(s) 881
Eam1104I CTCTTC 2 cut(s) 703, 1282
EarI CTCTTC 2 cut(s) 703, 1282
EciI GGCGGA 1 cut(s) 127
Eco130I CCWWGG 1 cut(s) 426
Eco147I AGGCCT 1 cut(s) 996
Eco31I GGTCTC 1 cut(s) 1037
Eco32I GATATC 1 cut(s) 654
Eco47I GGWCC 3 cut(s) 548, 773, 871
Eco57I CTGAAG 3 cut(s) 743, 786, 935
Eco81I CCTNAGG 1 cut(s) 1029
Eco88I CYCGRG 1 cut(s) 787
EcoO109I RGGNCCY 1 cut(s) 871
EcoRII CCWGG 4 cut(s) 663, 874, 976, 1208
EcoRV GATATC 1 cut(s) 654
EcoT14I CCWWGG 1 cut(s) 426
EcoT22I ATGCAT 1 cut(s) 755
ErhI CCWWGG 1 cut(s) 426
FaeI CATG 7 cut(s) 74, 377, 430, 753, 757, 827, 976
FaqI GGGAC 3 cut(s) 304, 955, 1084
FatI CATG 7 cut(s) 70, 373, 426, 749, 753, 823, 972
Fnu4HI GCNGC 4 cut(s) 75, 132, 639, 896
FokI GGATG 7 cut(s) 121, 269, 396, 443, 557, 891, 1036
Fsp4HI GCNGC 4 cut(s) 75, 132, 639, 896
FspBI CTAG 1 cut(s) 126
GluI GCNGC 4 cut(s) 75, 132, 639, 896
GsuI CTGGAG 1 cut(s) 831
HaeIII GGCC 4 cut(s) 88, 372, 883, 996
Hin1II CATG 7 cut(s) 74, 377, 430, 753, 757, 827, 976
HinfI GANTC 4 cut(s) 278, 466, 1013, 1213
HphI GGTGA 2 cut(s) 16, 1249
Hpy166II GTNNAC 3 cut(s) 123, 277, 1057
Hpy188I TCNGA 6 cut(s) 157, 253, 303, 805, 1087, 1132
Hpy188III TCNNGA 5 cut(s) 238, 733, 787, 789, 1021
Hpy8I GTNNAC 3 cut(s) 123, 277, 1057
HpyAV CCTTC 3 cut(s) 203, 786, 810
HpyCH4III ACNGT 4 cut(s) 43, 202, 689, 1196
HpyCH4V TGCA 7 cut(s) 83, 563, 753, 827, 915, 1115, 1219
HpyF10VI GCNNNNNNNGC 4 cut(s) 74, 80, 137, 1189
HpyF3I CTNAG 6 cut(s) 190, 644, 1019, 1029, 1041, 1247
Hsp92II CATG 7 cut(s) 74, 377, 430, 753, 757, 827, 976
Kzo9I GATC 7 cut(s) 240, 350, 907, 1171, 1231, 1243, 1264
LmnI GCTCC 2 cut(s) 82, 1081
Lsp1109I GCAGC 3 cut(s) 61, 625, 907
LweI GCATC 5 cut(s) 143, 385, 550, 1014, 1281
MaeI CTAG 1 cut(s) 126
MaeIII GTNAC 4 cut(s) 31, 421, 499, 934
MalI GATC 7 cut(s) 242, 352, 909, 1173, 1233, 1245, 1266
MboI GATC 7 cut(s) 240, 350, 907, 1171, 1231, 1243, 1264
MboII GAAGA 9 cut(s) 10, 99, 348, 569, 690, 775, 941, 1190, 1299
MflI RGATCY 4 cut(s) 240, 1171, 1231, 1243
MlsI TGGCCA 1 cut(s) 883
MluCI AATT 3 cut(s) 59, 483, 903
MluNI TGGCCA 1 cut(s) 883
MlyI GAGTC 1 cut(s) 272
Mox20I TGGCCA 1 cut(s) 883
Mph1103I ATGCAT 1 cut(s) 755
MscI TGGCCA 1 cut(s) 883
MseI TTAA 3 cut(s) 285, 1007, 1167
MslI CAYNNNNRTG 1 cut(s) 378
Msp20I TGGCCA 1 cut(s) 883
MspR9I CCNGG 4 cut(s) 665, 876, 978, 1210
MvaI CCWGG 4 cut(s) 665, 876, 978, 1210
MwoI GCNNNNNNNGC 4 cut(s) 74, 80, 137, 1189
NcoI CCATGG 1 cut(s) 426
NdeII GATC 7 cut(s) 240, 350, 907, 1171, 1231, 1243, 1264
NlaIII CATG 7 cut(s) 74, 377, 430, 753, 757, 827, 976
NlaIV GGNNCC 2 cut(s) 550, 873
NmeAIII GCCGAG 1 cut(s) 1072
NmuCI GTSAC 3 cut(s) 31, 499, 934
NsiI ATGCAT 1 cut(s) 755
NspI RCATGY 3 cut(s) 753, 757, 827
PaeI GCATGC 1 cut(s) 757
PaeR7I CTCGAG 1 cut(s) 787
PceI AGGCCT 1 cut(s) 996
PfeI GAWTC 3 cut(s) 466, 1013, 1213
PflMI CCANNNNNTGG 1 cut(s) 890
PkrI GCNGC 4 cut(s) 76, 133, 640, 897
PleI GAGTC 1 cut(s) 272
PpsI GAGTC 1 cut(s) 272
PpuMI RGGWCCY 1 cut(s) 871
PsiI TTATAA 1 cut(s) 902
Psp5II RGGWCCY 1 cut(s) 871
Psp6I CCWGG 4 cut(s) 663, 874, 976, 1208
PspGI CCWGG 4 cut(s) 663, 874, 976, 1208
PspN4I GGNNCC 2 cut(s) 550, 873
PspPI GGNCC 4 cut(s) 86, 548, 773, 871
PspPPI RGGWCCY 1 cut(s) 871
PstI CTGCAG 2 cut(s) 85, 1221
PsuI RGATCY 4 cut(s) 240, 1171, 1231, 1243
RsaI GTAC 2 cut(s) 188, 686
RsaNI GTAC 2 cut(s) 187, 685
RseI CAYNNNNRTG 1 cut(s) 378
SaqAI TTAA 3 cut(s) 285, 1007, 1167
SatI GCNGC 4 cut(s) 75, 132, 639, 896
Sau3AI GATC 7 cut(s) 240, 350, 907, 1171, 1231, 1243, 1264
Sau96I GGNCC 4 cut(s) 86, 548, 773, 871
SbfI CCTGCAGG 1 cut(s) 85
SchI GAGTC 1 cut(s) 272
ScrFI CCNGG 4 cut(s) 665, 876, 978, 1210
SdaI CCTGCAGG 1 cut(s) 85
SfaNI GCATC 5 cut(s) 143, 385, 550, 1014, 1281
SfcI CTRYAG 3 cut(s) 81, 538, 1217
Sfr274I CTCGAG 1 cut(s) 787
SinI GGWCC 3 cut(s) 548, 773, 871
SlaI CTCGAG 1 cut(s) 787
SmiMI CAYNNNNRTG 1 cut(s) 378
SmlI CTYRAG 1 cut(s) 787
SmoI CTYRAG 1 cut(s) 787
SphI GCATGC 1 cut(s) 757
Sse8387I CCTGCAGG 1 cut(s) 85
Sse9I AATT 3 cut(s) 59, 483, 903
SseBI AGGCCT 1 cut(s) 996
SsiI CCGC 3 cut(s) 131, 138, 244
SspI AATATT 1 cut(s) 529
SspMI CTAG 1 cut(s) 126
StuI AGGCCT 1 cut(s) 996
StyD4I CCNGG 4 cut(s) 663, 874, 976, 1208
StyI CCWWGG 1 cut(s) 426
TaaI ACNGT 4 cut(s) 43, 202, 689, 1196
TaqI TCGA 1 cut(s) 788
TasI AATT 3 cut(s) 59, 483, 903
TatI WGTACW 1 cut(s) 684
TauI GCSGC 1 cut(s) 134
TfiI GAWTC 3 cut(s) 466, 1013, 1213
Tru1I TTAA 3 cut(s) 285, 1007, 1167
Tru9I TTAA 3 cut(s) 285, 1007, 1167
TscAI CASTG 2 cut(s) 559, 939
TseFI GTSAC 3 cut(s) 31, 499, 934
TseI GCWGC 3 cut(s) 74, 638, 895
Tsp45I GTSAC 3 cut(s) 31, 499, 934
TspDTI ATGAA 5 cut(s) 10, 849, 946, 961, 1151
TspGWI ACGGA 2 cut(s) 271, 303
TspRI CASTG 2 cut(s) 559, 939
Van91I CCANNNNNTGG 1 cut(s) 890
VpaK11BI GGWCC 3 cut(s) 548, 773, 871
XceI RCATGY 3 cut(s) 753, 757, 827
XcmI CCANNNNNNNNNTGG 1 cut(s) 850
XhoI CTCGAG 1 cut(s) 787
XspI CTAG 1 cut(s) 126
Zsp2I ATGCAT 1 cut(s) 755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.