Rh5AG210700

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0010007
Physical Location & Seq
Reverse (-)
24863583 .. 24869126
5544 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG210700.1

Sequence Viewer

Length: 1431 bp
ATGACAAGTTCCCTTTTCTTCATCACCATCGTCACTCTCACAGTCGCTTACACCCTCCAATTCAAAGCCCATGCTGCTCCTGCAGGGCCATTGATAAAGCACTTGTCTTCCCTTGTCAAGTGGACTAGGGCGGCATCCGCCAAAGCACCCCAATCAGATGGGAATGTTGTTCAGTTTGAGAGTGGGTACTTAGTTGAGACTGTTGTGGAAGGAACTGATATTGGAGTTGTTCCTTTCAAGATCCGCATTTCAGAGGATGGCGAACTCTTTGCTGTGGACTCCGTTAATAGCAACATTGTTCGGATTACTCCTCCGTTGTCCCAATATAGTAGGGGAAGATTGGTTGCTGGATCATTTCAGGGTTATACAGGCCATGTTGATGGAAAGCCAAGTGATGCTCGTTTCAATCATCCAAAAGGTGTAACCATGGATGATAAAGGGAATGTATATGTTGCTGATACCTTGAATCACGCCATAAGAAAAATTGGAGAAGCTGGTGTGACAACCATAGCTGGTGGAAAATCAAATATTGCTGGCTACAGGGATGGACCCAGTGAAGATGCAATGTTCTCAAACGATTTTGATGTAGTATATGTCCAGCCTACTTGTTCCTTGCTTGTTGTTGATAGAGGAAATGCTGCTCTTAGGCAGATATCTCTCAACCAGGAGGATTGTGATTATCAGTACAGTTCAATCTCTTCCACAGATGTCCTTATGGTTGTTGGTGCTGTCTTGATAGGCTATGCTACATGCATGCTTCAGCAGGGATTTGGACCTTCTTTCTTCTCGAGAACGCAACCTTCAGAGAGTGAAACTAAAGAACATGCAAGCAATGAAAAATCCACTCCAGTTGTGGAGATTGTGAAAGAGGACCCAGGATGGCCATCTTTTGGGCAGCTTATAATTGATCTATGCAAGATTGGACTTGAAGCAGTGACGGGGACATTTCTTCAGTTCATTCCCTCTCGGTTCATGCCTGGTGGTTCTCACAAAGGCCTGACACCATTAAAAGATTCTCTCAGGATGCCTGAGGACGAAGCTGAGACCCCATTAGTTCACAGACAAAGCACTCCTGCTCCTCTGTCTGAAACTCGGCAGTCCCATACTCCCAATGCAAGTGATAGATATTCAGATATGAAGCCCTCAAAGATAAAGTCAAGTAGCTTTAAAGATCCCTCTTTGCCAAGCAAGCACCGGTCTTCAAAACGCCAGGATTCTGCAGAGTTCTATGGATCTGGTGAAAGATCTAAGAGCCAGAAAGAAAGATCACGGCATCGCCAACGAGAGAAGAGTGGAGAGGTAGTTTTTGGGGCCATTGGGACAGAACCGAAACCTGTGGACATGAAGACAGCAGCAGGTTTTGACAATCAGAAGTTTGAGAACTACAATATGAGGAGCAGGTATGGACCTGACAATTCATACCGGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NHL

Protein Analysis

476

Amino Acids

52.12

Weight (kDa)

7.18

Isoelectric Point (pI)

38.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NHL PF01436 135 - 161 6e-06 NHL repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 902
Acc36I ACCTGC 2 cut(s) 1346, 1389
AccB7I CCANNNNNTGG 1 cut(s) 890
AciI CCGC 3 cut(s) 131, 138, 244
AclWI GGATC 4 cut(s) 235, 358, 1166, 1240
AcoI YGGCCR 1 cut(s) 881
AcuI CTGAAG 3 cut(s) 743, 786, 935
AfaI GTAC 2 cut(s) 188, 686
AfiI CCNNNNNNNGG 1 cut(s) 890
AgeI ACCGGT 2 cut(s) 1194, 1422
AgsI TTSAA 7 cut(s) 64, 238, 406, 466, 693, 929, 1203
AjnI CCWGG 4 cut(s) 663, 874, 976, 1209
AluBI AGCT 5 cut(s) 494, 512, 898, 1040, 1164
AluI AGCT 5 cut(s) 494, 512, 898, 1040, 1164
Alw26I GTCTC 2 cut(s) 191, 1037
AlwI GGATC 4 cut(s) 235, 358, 1166, 1240
Ama87I CYCGRG 1 cut(s) 787
AoxI GGCC 5 cut(s) 86, 370, 881, 994, 1311
ApeKI GCWGC 4 cut(s) 74, 638, 895, 1352
ArsI GACNNNNNNTTYG 2 cut(s) 1139, 1171
AsiGI ACCGGT 2 cut(s) 1194, 1422
AspS9I GGNCC 6 cut(s) 86, 548, 773, 871, 1311, 1406
AsuHPI GGTGA 2 cut(s) 16, 1250
AvaI CYCGRG 1 cut(s) 787
AvaII GGWCC 4 cut(s) 548, 773, 871, 1406
AxyI CCTNAGG 1 cut(s) 1029
BalI TGGCCA 1 cut(s) 883
BbsI GAAGAC 3 cut(s) 99, 1191, 1352
BbvI GCAGC 4 cut(s) 61, 625, 907, 1364
BccI CCATC 7 cut(s) 35, 152, 251, 374, 539, 873, 892
BceAI ACGGC 1 cut(s) 1286
BcgI CGANNNNNNTGC 2 cut(s) 251, 285
BciT130I CCWGG 4 cut(s) 665, 876, 978, 1211
BcoDI GTCTC 2 cut(s) 191, 1037
BfaI CTAG 1 cut(s) 126
BfmI CTRYAG 3 cut(s) 81, 538, 1218
BfuAI ACCTGC 2 cut(s) 1346, 1389
BglII AGATCT 1 cut(s) 1244
BisI GCNGC 5 cut(s) 75, 132, 639, 896, 1353
BlsI GCNGC 5 cut(s) 76, 133, 640, 897, 1354
Bme1390I CCNGG 4 cut(s) 665, 876, 978, 1211
Bme18I GGWCC 4 cut(s) 548, 773, 871, 1406
BmeT110I CYCGRG 1 cut(s) 787
BmgT120I GGNCC 6 cut(s) 86, 548, 773, 871, 1311, 1406
BmiI GGNNCC 3 cut(s) 550, 873, 1312
BmrFI CCNGG 4 cut(s) 665, 876, 978, 1211
BmrI ACTGGG 1 cut(s) 546
BmsI GCATC 5 cut(s) 143, 385, 550, 1014, 1282
BmuI ACTGGG 1 cut(s) 546
BpiI GAAGAC 3 cut(s) 99, 1191, 1352
BpmI CTGGAG 1 cut(s) 831
BsaBI GATNNNNATC 1 cut(s) 883
BsaI GGTCTC 1 cut(s) 1037
BsaJI CCNNGG 2 cut(s) 426, 874
BsaWI WCCGGW 2 cut(s) 1194, 1422
BsaXI ACNNNNNCTCC 4 cut(s) 1060, 1090, 1287, 1317
Bsc4I CCNNNNNNNGG 1 cut(s) 890
Bse118I RCCGGY 2 cut(s) 1194, 1422
Bse1I ACTGG 2 cut(s) 552, 848
Bse21I CCTNAGG 1 cut(s) 1029
Bse3DI GCAATG 2 cut(s) 570, 838
Bse8I GATNNNNATC 1 cut(s) 883
BseBI CCWGG 4 cut(s) 665, 876, 978, 1211
BseDI CCNNGG 2 cut(s) 426, 874
BseGI GGATG 7 cut(s) 134, 262, 409, 436, 550, 884, 1029
BseJI GATNNNNATC 1 cut(s) 883
BseLI CCNNNNNNNGG 1 cut(s) 890
BseMI GCAATG 2 cut(s) 570, 838
BseMII CTCAG 3 cut(s) 1020, 1032, 1033
BseNI ACTGG 2 cut(s) 552, 848
BseRI GAGGAG 3 cut(s) 300, 1068, 1408
BseXI GCAGC 4 cut(s) 61, 625, 907, 1364
BshFI GGCC 5 cut(s) 88, 372, 883, 996, 1313
BshTI ACCGGT 2 cut(s) 1194, 1422
BsiHKCI CYCGRG 1 cut(s) 787
BsiSI CCGG 2 cut(s) 1195, 1423
BslFI GGGAC 4 cut(s) 304, 955, 1084, 1333
BslI CCNNNNNNNGG 1 cut(s) 890
BsmAI GTCTC 2 cut(s) 191, 1037
BsmFI GGGAC 4 cut(s) 304, 955, 1084, 1333
BsnI GGCC 5 cut(s) 88, 372, 883, 996, 1313
Bso31I GGTCTC 1 cut(s) 1037
BsoBI CYCGRG 1 cut(s) 787
Bsp143I GATC 7 cut(s) 240, 350, 907, 1171, 1232, 1244, 1265
Bsp19I CCATGG 1 cut(s) 426
BspACI CCGC 3 cut(s) 131, 138, 244
BspANI GGCC 5 cut(s) 88, 372, 883, 996, 1313
BspCNI CTCAG 3 cut(s) 1021, 1032, 1033
BspLI GGNNCC 3 cut(s) 550, 873, 1312
BspMAI CTGCAG 2 cut(s) 85, 1222
BspMI ACCTGC 2 cut(s) 1346, 1389
BspPI GGATC 4 cut(s) 235, 358, 1166, 1240
BspTNI GGTCTC 1 cut(s) 1037
BsrDI GCAATG 2 cut(s) 570, 838
BsrFI RCCGGY 2 cut(s) 1194, 1422
BsrI ACTGG 2 cut(s) 552, 848
BssAI RCCGGY 2 cut(s) 1194, 1422
BssECI CCNNGG 2 cut(s) 426, 874
BssMI GATC 7 cut(s) 240, 350, 907, 1171, 1232, 1244, 1265
BssT1I CCWWGG 1 cut(s) 426
Bst2UI CCWGG 4 cut(s) 665, 876, 978, 1211
Bst4CI ACNGT 3 cut(s) 43, 202, 689
Bst6I CTCTTC 2 cut(s) 703, 1283
BstC8I GCNNGC 4 cut(s) 535, 755, 829, 1190
BstDEI CTNAG 6 cut(s) 190, 644, 1019, 1029, 1041, 1248
BstDSI CCRYGG 1 cut(s) 426
BstF5I GGATG 7 cut(s) 134, 262, 409, 436, 550, 884, 1029
BstKTI GATC 7 cut(s) 243, 353, 910, 1174, 1235, 1247, 1268
BstMAI GTCTC 2 cut(s) 191, 1037
BstMBI GATC 7 cut(s) 240, 350, 907, 1171, 1232, 1244, 1265
BstMWI GCNNNNNNNGC 4 cut(s) 74, 80, 137, 1189
BstNI CCWGG 4 cut(s) 665, 876, 978, 1211
BstNSI RCATGY 3 cut(s) 753, 757, 827
BstSCI CCNGG 4 cut(s) 663, 874, 976, 1209
BstSFI CTRYAG 3 cut(s) 81, 538, 1218
BstV1I GCAGC 4 cut(s) 61, 625, 907, 1364
BstV2I GAAGAC 3 cut(s) 99, 1191, 1352
BstX2I RGATCY 4 cut(s) 240, 1171, 1232, 1244
BstXI CCANNNNNNTGG 2 cut(s) 158, 380
BstYI RGATCY 4 cut(s) 240, 1171, 1232, 1244
Bsu36I CCTNAGG 1 cut(s) 1029
BsuRI GGCC 5 cut(s) 88, 372, 883, 996, 1313
BtgI CCRYGG 1 cut(s) 426
BtgZI GCGATG 1 cut(s) 1259
BtsCI GGATG 7 cut(s) 134, 262, 409, 436, 550, 884, 1029
BtsI GCAGTG 1 cut(s) 939
BtsIMutI CAGTG 2 cut(s) 559, 939
BveI ACCTGC 2 cut(s) 1346, 1389
Cac8I GCNNGC 4 cut(s) 535, 755, 829, 1190
Cfr10I RCCGGY 2 cut(s) 1194, 1422
Cfr13I GGNCC 6 cut(s) 86, 548, 773, 871, 1311, 1406
Csp6I GTAC 2 cut(s) 187, 685
CspAI ACCGGT 2 cut(s) 1194, 1422
CspCI CAANNNNNGTGG 2 cut(s) 832, 867
CviAII CATG 8 cut(s) 71, 374, 427, 750, 754, 824, 973, 1342
CviQI GTAC 2 cut(s) 187, 685
DdeI CTNAG 6 cut(s) 190, 644, 1019, 1029, 1041, 1248
DpnI GATC 7 cut(s) 242, 352, 909, 1173, 1234, 1246, 1267
DpnII GATC 7 cut(s) 240, 350, 907, 1171, 1232, 1244, 1265
DraI TTTAAA 1 cut(s) 1168
EaeI YGGCCR 1 cut(s) 881
Eam1104I CTCTTC 2 cut(s) 703, 1283
EarI CTCTTC 2 cut(s) 703, 1283
EciI GGCGGA 1 cut(s) 127
Eco130I CCWWGG 1 cut(s) 426
Eco147I AGGCCT 1 cut(s) 996
Eco31I GGTCTC 1 cut(s) 1037
Eco32I GATATC 1 cut(s) 654
Eco47I GGWCC 4 cut(s) 548, 773, 871, 1406
Eco57I CTGAAG 3 cut(s) 743, 786, 935
Eco81I CCTNAGG 1 cut(s) 1029
Eco88I CYCGRG 1 cut(s) 787
EcoO109I RGGNCCY 1 cut(s) 871
EcoRII CCWGG 4 cut(s) 663, 874, 976, 1209
EcoRV GATATC 1 cut(s) 654
EcoT14I CCWWGG 1 cut(s) 426
EcoT22I ATGCAT 1 cut(s) 755
ErhI CCWWGG 1 cut(s) 426
FaeI CATG 8 cut(s) 74, 377, 430, 753, 757, 827, 976, 1345
FaqI GGGAC 4 cut(s) 304, 955, 1084, 1333
FatI CATG 8 cut(s) 70, 373, 426, 749, 753, 823, 972, 1341
Fnu4HI GCNGC 5 cut(s) 75, 132, 639, 896, 1353
FokI GGATG 7 cut(s) 121, 269, 396, 443, 557, 891, 1036
Fsp4HI GCNGC 5 cut(s) 75, 132, 639, 896, 1353
FspBI CTAG 1 cut(s) 126
GluI GCNGC 5 cut(s) 75, 132, 639, 896, 1353
GsuI CTGGAG 1 cut(s) 831
HaeIII GGCC 5 cut(s) 88, 372, 883, 996, 1313
HapII CCGG 2 cut(s) 1195, 1423
Hin1II CATG 8 cut(s) 74, 377, 430, 753, 757, 827, 976, 1345
HinfI GANTC 4 cut(s) 278, 466, 1013, 1214
HpaII CCGG 2 cut(s) 1195, 1423
HphI GGTGA 2 cut(s) 16, 1250
Hpy166II GTNNAC 4 cut(s) 123, 277, 1057, 1339
Hpy188I TCNGA 7 cut(s) 157, 253, 303, 805, 1087, 1132, 1371
Hpy188III TCNNGA 5 cut(s) 238, 733, 787, 789, 1021
Hpy8I GTNNAC 4 cut(s) 123, 277, 1057, 1339
HpyAV CCTTC 3 cut(s) 203, 786, 810
HpyCH4III ACNGT 3 cut(s) 43, 202, 689
HpyCH4V TGCA 7 cut(s) 83, 563, 753, 827, 915, 1115, 1220
HpyF10VI GCNNNNNNNGC 4 cut(s) 74, 80, 137, 1189
HpyF3I CTNAG 6 cut(s) 190, 644, 1019, 1029, 1041, 1248
Hsp92II CATG 8 cut(s) 74, 377, 430, 753, 757, 827, 976, 1345
Kzo9I GATC 7 cut(s) 240, 350, 907, 1171, 1232, 1244, 1265
LmnI GCTCC 3 cut(s) 82, 1081, 1395
Lsp1109I GCAGC 4 cut(s) 61, 625, 907, 1364
LweI GCATC 5 cut(s) 143, 385, 550, 1014, 1282
MaeI CTAG 1 cut(s) 126
MaeIII GTNAC 4 cut(s) 31, 421, 499, 934
MalI GATC 7 cut(s) 242, 352, 909, 1173, 1234, 1246, 1267
MboI GATC 7 cut(s) 240, 350, 907, 1171, 1232, 1244, 1265
MflI RGATCY 4 cut(s) 240, 1171, 1232, 1244
MlsI TGGCCA 1 cut(s) 883
MluCI AATT 4 cut(s) 59, 483, 903, 1414
MluNI TGGCCA 1 cut(s) 883
MlyI GAGTC 1 cut(s) 272
Mox20I TGGCCA 1 cut(s) 883
Mph1103I ATGCAT 1 cut(s) 755
MscI TGGCCA 1 cut(s) 883
MseI TTAA 4 cut(s) 285, 1007, 1167, 1429
MslI CAYNNNNRTG 1 cut(s) 378
Msp20I TGGCCA 1 cut(s) 883
MspI CCGG 2 cut(s) 1195, 1423
MspR9I CCNGG 4 cut(s) 665, 876, 978, 1211
MvaI CCWGG 4 cut(s) 665, 876, 978, 1211
MwoI GCNNNNNNNGC 4 cut(s) 74, 80, 137, 1189
NcoI CCATGG 1 cut(s) 426
NdeII GATC 7 cut(s) 240, 350, 907, 1171, 1232, 1244, 1265
NlaIII CATG 8 cut(s) 74, 377, 430, 753, 757, 827, 976, 1345
NlaIV GGNNCC 3 cut(s) 550, 873, 1312
NmeAIII GCCGAG 1 cut(s) 1072
NmuCI GTSAC 3 cut(s) 31, 499, 934
NsiI ATGCAT 1 cut(s) 755
NspI RCATGY 3 cut(s) 753, 757, 827
PaeI GCATGC 1 cut(s) 757
PaeR7I CTCGAG 1 cut(s) 787
PceI AGGCCT 1 cut(s) 996
PfeI GAWTC 3 cut(s) 466, 1013, 1214
PflMI CCANNNNNTGG 1 cut(s) 890
PinAI ACCGGT 2 cut(s) 1194, 1422
PkrI GCNGC 5 cut(s) 76, 133, 640, 897, 1354
PleI GAGTC 1 cut(s) 272
PpsI GAGTC 1 cut(s) 272
PpuMI RGGWCCY 1 cut(s) 871
PsiI TTATAA 1 cut(s) 902
Psp5II RGGWCCY 1 cut(s) 871
Psp6I CCWGG 4 cut(s) 663, 874, 976, 1209
PspGI CCWGG 4 cut(s) 663, 874, 976, 1209
PspN4I GGNNCC 3 cut(s) 550, 873, 1312
PspPI GGNCC 6 cut(s) 86, 548, 773, 871, 1311, 1406
PspPPI RGGWCCY 1 cut(s) 871
PstI CTGCAG 2 cut(s) 85, 1222
PsuI RGATCY 4 cut(s) 240, 1171, 1232, 1244
RsaI GTAC 2 cut(s) 188, 686
RsaNI GTAC 2 cut(s) 187, 685
RseI CAYNNNNRTG 1 cut(s) 378
SaqAI TTAA 4 cut(s) 285, 1007, 1167, 1429
SatI GCNGC 5 cut(s) 75, 132, 639, 896, 1353
Sau3AI GATC 7 cut(s) 240, 350, 907, 1171, 1232, 1244, 1265
Sau96I GGNCC 6 cut(s) 86, 548, 773, 871, 1311, 1406
SbfI CCTGCAGG 1 cut(s) 85
SchI GAGTC 1 cut(s) 272
ScrFI CCNGG 4 cut(s) 665, 876, 978, 1211
SdaI CCTGCAGG 1 cut(s) 85
SfaNI GCATC 5 cut(s) 143, 385, 550, 1014, 1282
SfcI CTRYAG 3 cut(s) 81, 538, 1218
Sfr274I CTCGAG 1 cut(s) 787
SinI GGWCC 4 cut(s) 548, 773, 871, 1406
SlaI CTCGAG 1 cut(s) 787
SmiMI CAYNNNNRTG 1 cut(s) 378
SmlI CTYRAG 1 cut(s) 787
SmoI CTYRAG 1 cut(s) 787
SphI GCATGC 1 cut(s) 757
Sse8387I CCTGCAGG 1 cut(s) 85
Sse9I AATT 4 cut(s) 59, 483, 903, 1414
SseBI AGGCCT 1 cut(s) 996
SsiI CCGC 3 cut(s) 131, 138, 244
SspI AATATT 1 cut(s) 529
SspMI CTAG 1 cut(s) 126
StuI AGGCCT 1 cut(s) 996
StyD4I CCNGG 4 cut(s) 663, 874, 976, 1209
StyI CCWWGG 1 cut(s) 426
TaaI ACNGT 3 cut(s) 43, 202, 689
TaqI TCGA 1 cut(s) 788
TasI AATT 4 cut(s) 59, 483, 903, 1414
TatI WGTACW 1 cut(s) 684
TauI GCSGC 1 cut(s) 134
TfiI GAWTC 3 cut(s) 466, 1013, 1214
Tru1I TTAA 4 cut(s) 285, 1007, 1167, 1429
Tru9I TTAA 4 cut(s) 285, 1007, 1167, 1429
TscAI CASTG 2 cut(s) 559, 939
TseFI GTSAC 3 cut(s) 31, 499, 934
TseI GCWGC 4 cut(s) 74, 638, 895, 1352
Tsp45I GTSAC 3 cut(s) 31, 499, 934
TspDTI ATGAA 7 cut(s) 10, 849, 946, 961, 1151, 1358, 1407
TspGWI ACGGA 2 cut(s) 271, 303
TspRI CASTG 2 cut(s) 559, 939
Van91I CCANNNNNTGG 1 cut(s) 890
VpaK11BI GGWCC 4 cut(s) 548, 773, 871, 1406
XceI RCATGY 3 cut(s) 753, 757, 827
XcmI CCANNNNNNNNNTGG 1 cut(s) 850
XhoI CTCGAG 1 cut(s) 787
XspI CTAG 1 cut(s) 126
Zsp2I ATGCAT 1 cut(s) 755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.