Rroxscaffold_1G00049730

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0010007
Physical Location & Seq
Forward (+)
70097307 .. 70102257
4951 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00049730.1

Sequence Viewer

Length: 1371 bp
ATGAGAAGTTCCCTTTTCTTCATCACCATCGTCACTCTCACAGTCGCTTACACCCTCCAATTCAAAGCCCATGCTGCTCCTGCAGGGCCATTGATAAAGCACTTGTCTTCCCTTGTCAAGTGGACGAGGGCGGCATCCGCCAAAGCACCCCAATCAGATGGGAATGTTGTTCAGTTTGAGAGTGGGTACTTAGTTGAGACTGTTGTGGAAGGAACTGATATTGGAGTTGTTCCTTTCAAGATCCGCATTTCAGAGGATGGCGAACTCTTTGCTGTGGACTCCGTTAATAGCAACATTGGTTATACGGGCCATGTTGATGGAAAGCCAAGTGATGCTCGTTTCAATCATCCAAAAGGTGTAACCATGGATGATAAAGGGAATGTATATGTTGCTGATACCTTGAATCATGCCATAAGAAAAATTGGAGAAGCTGGTGTGACAACCATAGCTGGTGGAAAATCAAATATTGCTGGCTACAGGGATGGACCCAGTGAAGATGCAATGTTCTCAAACGATTTTGATGTAGTATATGTCCAGCCTACTTGTTCCTTGCTTGTTGTTGATAGAGGAAATGCTGCTCTTAGGCAGATATCTCTCAACCAGGAGGATTGTGATTATCAGTACAGTTCAATCTCTTCCACAGATGTCCTTATGGTTGTTGGTGCTGTCTTGATAGGCTATGCTACATGCATGCTTCAGCAGGGATTTGGACCTTCTTTCTTCTCGAGAATGCAGCAACCTTCAGAGAGTGAAACTAAAGAACATGCAAGCAATGAAAAATCCACTCCAGTGGTGGAGATTGTGAAAGAGGACCCAGGATGGCCATCTTTTGGGCAGCTTATAATTGATCTATGCAAGATTGGACTTGAAGCAGTGACGGGCACATTTCTTCAGTTCATTCCCTCTCGTTTCATGCCTGGTGGTTCTCACAAAGGCCTGACACCATTAAAAGATTCTCTCAGGATGCCTGAGGACGAAGCTGAGACCCCGTTAGTTCACAGGCAAAGCACGCCTGCTCCTCTGTCTGAAACTCGGCAGTCCCATACTCCCAATGCAAGTGATAGATATTCAGATATGAAGCCCTCAAAGATAAAGTCAAGTAGCTTTAAAGACCCCTCTTTGCCAAGCAAGCACCGGTCTTCAAAACGCCAGGATTCTGCAGAATTCTATGGATCTGGTGAAAGATCTAAGAGCCAGAAAGAAAGAACACGGCATCGCCAACGAGAGAAGAGTGGAGAGGGAGTTTTTGTGGCCGTTGGGACAGAATCGAAACCTGTGGACATGAAGACAGCAGCAGGTTATGACAATCAGAAGTTTGAGAACTACAATATGAGGAGCAGGTATGGACCTGACAATTCATACCGGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NHL

Protein Analysis

456

Amino Acids

50.03

Weight (kDa)

6.61

Isoelectric Point (pI)

39.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NHL PF01436 114 - 140 5.7e-06 NHL repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 842
Acc36I ACCTGC 2 cut(s) 1286, 1329
AccB7I CCANNNNNTGG 1 cut(s) 830
AciI CCGC 3 cut(s) 131, 138, 244
AclWI GGATC 2 cut(s) 235, 1180
AcoI YGGCCR 2 cut(s) 821, 1251
AcsI RAATTY 1 cut(s) 1163
AcuI CTGAAG 3 cut(s) 680, 726, 875
AfaI GTAC 2 cut(s) 188, 623
AfiI CCNNNNNNNGG 1 cut(s) 830
AgeI ACCGGT 2 cut(s) 1134, 1362
AgsI TTSAA 7 cut(s) 64, 238, 343, 403, 630, 869, 1143
AjnI CCWGG 4 cut(s) 600, 814, 916, 1149
AleI CACNNNNGTG 1 cut(s) 788
AluBI AGCT 5 cut(s) 431, 449, 838, 980, 1104
AluI AGCT 5 cut(s) 431, 449, 838, 980, 1104
Alw26I GTCTC 2 cut(s) 191, 977
AlwI GGATC 2 cut(s) 235, 1180
Ama87I CYCGRG 1 cut(s) 724
AoxI GGCC 5 cut(s) 86, 307, 821, 934, 1251
ApeKI GCWGC 5 cut(s) 74, 575, 733, 835, 1292
ApoI RAATTY 1 cut(s) 1163
ArsI GACNNNNNNTTYG 2 cut(s) 1079, 1111
AsiGI ACCGGT 2 cut(s) 1134, 1362
AspS9I GGNCC 6 cut(s) 86, 307, 485, 710, 811, 1346
AsuHPI GGTGA 2 cut(s) 16, 1190
AvaI CYCGRG 1 cut(s) 724
AvaII GGWCC 4 cut(s) 485, 710, 811, 1346
AxyI CCTNAGG 1 cut(s) 969
BaeGI GKGCMC 1 cut(s) 884
BalI TGGCCA 1 cut(s) 823
BbsI GAAGAC 3 cut(s) 99, 1131, 1292
BbvI GCAGC 5 cut(s) 61, 562, 745, 847, 1304
BccI CCATC 7 cut(s) 35, 152, 251, 311, 476, 813, 832
BceAI ACGGC 2 cut(s) 1226, 1238
BcgI CGANNNNNNTGC 2 cut(s) 251, 285
BciT130I CCWGG 4 cut(s) 602, 816, 918, 1151
BcoDI GTCTC 2 cut(s) 191, 977
BfmI CTRYAG 3 cut(s) 81, 475, 1158
BfuAI ACCTGC 2 cut(s) 1286, 1329
BglII AGATCT 1 cut(s) 1184
BisI GCNGC 6 cut(s) 75, 132, 576, 734, 836, 1293
BlsI GCNGC 6 cut(s) 76, 133, 577, 735, 837, 1294
Bme1390I CCNGG 4 cut(s) 602, 816, 918, 1151
Bme18I GGWCC 4 cut(s) 485, 710, 811, 1346
BmeT110I CYCGRG 1 cut(s) 724
BmgT120I GGNCC 6 cut(s) 86, 307, 485, 710, 811, 1346
BmiI GGNNCC 2 cut(s) 487, 813
BmrFI CCNGG 4 cut(s) 602, 816, 918, 1151
BmrI ACTGGG 1 cut(s) 483
BmsI GCATC 5 cut(s) 143, 322, 487, 954, 1222
BmuI ACTGGG 1 cut(s) 483
BpiI GAAGAC 3 cut(s) 99, 1131, 1292
BpmI CTGGAG 1 cut(s) 771
BsaBI GATNNNNATC 1 cut(s) 823
BsaI GGTCTC 1 cut(s) 977
BsaJI CCNNGG 2 cut(s) 363, 814
BsaWI WCCGGW 2 cut(s) 1134, 1362
BsaXI ACNNNNNCTCC 6 cut(s) 1000, 1030, 1227, 1233, 1257, 1263
Bsc4I CCNNNNNNNGG 1 cut(s) 830
Bse118I RCCGGY 2 cut(s) 1134, 1362
Bse1I ACTGG 2 cut(s) 489, 788
Bse21I CCTNAGG 1 cut(s) 969
Bse3DI GCAATG 2 cut(s) 507, 778
Bse8I GATNNNNATC 1 cut(s) 823
BseBI CCWGG 4 cut(s) 602, 816, 918, 1151
BseDI CCNNGG 2 cut(s) 363, 814
BseGI GGATG 7 cut(s) 134, 262, 346, 373, 487, 824, 969
BseJI GATNNNNATC 1 cut(s) 823
BseLI CCNNNNNNNGG 1 cut(s) 830
BseMI GCAATG 2 cut(s) 507, 778
BseMII CTCAG 3 cut(s) 960, 972, 973
BseNI ACTGG 2 cut(s) 489, 788
BseRI GAGGAG 2 cut(s) 1008, 1348
BseSI GKGCMC 1 cut(s) 884
BseXI GCAGC 5 cut(s) 61, 562, 745, 847, 1304
BshFI GGCC 5 cut(s) 88, 309, 823, 936, 1253
BshTI ACCGGT 2 cut(s) 1134, 1362
BsiHKCI CYCGRG 1 cut(s) 724
BsiSI CCGG 2 cut(s) 1135, 1363
BslFI GGGAC 2 cut(s) 1024, 1273
BslI CCNNNNNNNGG 1 cut(s) 830
BsmAI GTCTC 2 cut(s) 191, 977
BsmFI GGGAC 2 cut(s) 1024, 1273
BsmI GAATGC 1 cut(s) 735
BsnI GGCC 5 cut(s) 88, 309, 823, 936, 1253
Bso31I GGTCTC 1 cut(s) 977
BsoBI CYCGRG 1 cut(s) 724
Bsp1286I GDGCHC 1 cut(s) 884
Bsp143I GATC 4 cut(s) 240, 847, 1172, 1184
Bsp19I CCATGG 1 cut(s) 363
BspACI CCGC 3 cut(s) 131, 138, 244
BspANI GGCC 5 cut(s) 88, 309, 823, 936, 1253
BspCNI CTCAG 3 cut(s) 961, 972, 973
BspLI GGNNCC 2 cut(s) 487, 813
BspMAI CTGCAG 2 cut(s) 85, 1162
BspMI ACCTGC 2 cut(s) 1286, 1329
BspPI GGATC 2 cut(s) 235, 1180
BspTNI GGTCTC 1 cut(s) 977
BsrDI GCAATG 2 cut(s) 507, 778
BsrFI RCCGGY 2 cut(s) 1134, 1362
BsrI ACTGG 2 cut(s) 489, 788
BssAI RCCGGY 2 cut(s) 1134, 1362
BssECI CCNNGG 2 cut(s) 363, 814
BssMI GATC 4 cut(s) 240, 847, 1172, 1184
BssT1I CCWWGG 1 cut(s) 363
Bst2UI CCWGG 4 cut(s) 602, 816, 918, 1151
Bst4CI ACNGT 3 cut(s) 43, 202, 626
Bst6I CTCTTC 2 cut(s) 640, 1223
BstC8I GCNNGC 6 cut(s) 472, 692, 769, 1010, 1014, 1130
BstDEI CTNAG 6 cut(s) 190, 581, 959, 969, 981, 1188
BstDSI CCRYGG 1 cut(s) 363
BstF5I GGATG 7 cut(s) 134, 262, 346, 373, 487, 824, 969
BstKTI GATC 4 cut(s) 243, 850, 1175, 1187
BstMAI GTCTC 2 cut(s) 191, 977
BstMBI GATC 4 cut(s) 240, 847, 1172, 1184
BstMWI GCNNNNNNNGC 5 cut(s) 74, 80, 137, 1009, 1129
BstNI CCWGG 4 cut(s) 602, 816, 918, 1151
BstNSI RCATGY 3 cut(s) 690, 694, 767
BstSCI CCNGG 4 cut(s) 600, 814, 916, 1149
BstSFI CTRYAG 3 cut(s) 81, 475, 1158
BstSLI GKGCMC 1 cut(s) 884
BstV1I GCAGC 5 cut(s) 61, 562, 745, 847, 1304
BstV2I GAAGAC 3 cut(s) 99, 1131, 1292
BstX2I RGATCY 3 cut(s) 240, 1172, 1184
BstXI CCANNNNNNTGG 3 cut(s) 158, 317, 790
BstYI RGATCY 3 cut(s) 240, 1172, 1184
Bsu36I CCTNAGG 1 cut(s) 969
BsuRI GGCC 5 cut(s) 88, 309, 823, 936, 1253
BtgI CCRYGG 1 cut(s) 363
BtgZI GCGATG 1 cut(s) 1199
BtsCI GGATG 7 cut(s) 134, 262, 346, 373, 487, 824, 969
BtsI GCAGTG 1 cut(s) 879
BtsIMutI CAGTG 3 cut(s) 496, 795, 879
BveI ACCTGC 2 cut(s) 1286, 1329
Cac8I GCNNGC 6 cut(s) 472, 692, 769, 1010, 1014, 1130
Cfr10I RCCGGY 2 cut(s) 1134, 1362
Cfr13I GGNCC 6 cut(s) 86, 307, 485, 710, 811, 1346
Csp6I GTAC 2 cut(s) 187, 622
CspAI ACCGGT 2 cut(s) 1134, 1362
CviAII CATG 9 cut(s) 71, 311, 364, 407, 687, 691, 764, 913, 1282
CviQI GTAC 2 cut(s) 187, 622
DdeI CTNAG 6 cut(s) 190, 581, 959, 969, 981, 1188
DpnI GATC 4 cut(s) 242, 849, 1174, 1186
DpnII GATC 4 cut(s) 240, 847, 1172, 1184
DraI TTTAAA 1 cut(s) 1108
EaeI YGGCCR 2 cut(s) 821, 1251
Eam1104I CTCTTC 2 cut(s) 640, 1223
EarI CTCTTC 2 cut(s) 640, 1223
EciI GGCGGA 1 cut(s) 127
Eco130I CCWWGG 1 cut(s) 363
Eco147I AGGCCT 1 cut(s) 936
Eco31I GGTCTC 1 cut(s) 977
Eco32I GATATC 1 cut(s) 591
Eco47I GGWCC 4 cut(s) 485, 710, 811, 1346
Eco57I CTGAAG 3 cut(s) 680, 726, 875
Eco81I CCTNAGG 1 cut(s) 969
Eco88I CYCGRG 1 cut(s) 724
EcoO109I RGGNCCY 1 cut(s) 811
EcoRI GAATTC 1 cut(s) 1163
EcoRII CCWGG 4 cut(s) 600, 814, 916, 1149
EcoRV GATATC 1 cut(s) 591
EcoT14I CCWWGG 1 cut(s) 363
EcoT22I ATGCAT 1 cut(s) 692
ErhI CCWWGG 1 cut(s) 363
FaeI CATG 9 cut(s) 74, 314, 367, 410, 690, 694, 767, 916, 1285
FaqI GGGAC 2 cut(s) 1024, 1273
FatI CATG 9 cut(s) 70, 310, 363, 406, 686, 690, 763, 912, 1281
Fnu4HI GCNGC 6 cut(s) 75, 132, 576, 734, 836, 1293
FokI GGATG 7 cut(s) 121, 269, 333, 380, 494, 831, 976
Fsp4HI GCNGC 6 cut(s) 75, 132, 576, 734, 836, 1293
GluI GCNGC 6 cut(s) 75, 132, 576, 734, 836, 1293
GsuI CTGGAG 1 cut(s) 771
HaeIII GGCC 5 cut(s) 88, 309, 823, 936, 1253
HapII CCGG 2 cut(s) 1135, 1363
Hin1II CATG 9 cut(s) 74, 314, 367, 410, 690, 694, 767, 916, 1285
HinfI GANTC 5 cut(s) 278, 403, 953, 1154, 1265
HpaII CCGG 2 cut(s) 1135, 1363
HphI GGTGA 2 cut(s) 16, 1190
Hpy166II GTNNAC 4 cut(s) 123, 277, 997, 1279
Hpy188I TCNGA 6 cut(s) 157, 253, 745, 1027, 1072, 1311
Hpy188III TCNNGA 5 cut(s) 238, 670, 724, 726, 961
Hpy8I GTNNAC 4 cut(s) 123, 277, 997, 1279
HpyAV CCTTC 3 cut(s) 203, 723, 750
HpyCH4III ACNGT 3 cut(s) 43, 202, 626
HpyCH4V TGCA 8 cut(s) 83, 500, 690, 733, 767, 855, 1055, 1160
HpyF10VI GCNNNNNNNGC 5 cut(s) 74, 80, 137, 1009, 1129
HpyF3I CTNAG 6 cut(s) 190, 581, 959, 969, 981, 1188
Hsp92II CATG 9 cut(s) 74, 314, 367, 410, 690, 694, 767, 916, 1285
Kzo9I GATC 4 cut(s) 240, 847, 1172, 1184
LmnI GCTCC 3 cut(s) 82, 1021, 1335
Lsp1109I GCAGC 5 cut(s) 61, 562, 745, 847, 1304
LweI GCATC 5 cut(s) 143, 322, 487, 954, 1222
MaeIII GTNAC 4 cut(s) 31, 358, 436, 874
MalI GATC 4 cut(s) 242, 849, 1174, 1186
MboI GATC 4 cut(s) 240, 847, 1172, 1184
MboII GAAGA 9 cut(s) 10, 99, 506, 627, 712, 881, 1131, 1240, 1297
MflI RGATCY 3 cut(s) 240, 1172, 1184
MhlI GDGCHC 1 cut(s) 884
MlsI TGGCCA 1 cut(s) 823
MluCI AATT 5 cut(s) 59, 420, 843, 1163, 1354
MluNI TGGCCA 1 cut(s) 823
MlyI GAGTC 1 cut(s) 272
Mox20I TGGCCA 1 cut(s) 823
Mph1103I ATGCAT 1 cut(s) 692
MscI TGGCCA 1 cut(s) 823
MseI TTAA 4 cut(s) 285, 947, 1107, 1369
MslI CAYNNNNRTG 2 cut(s) 315, 788
Msp20I TGGCCA 1 cut(s) 823
MspI CCGG 2 cut(s) 1135, 1363
MspR9I CCNGG 4 cut(s) 602, 816, 918, 1151
Mva1269I GAATGC 1 cut(s) 735
MvaI CCWGG 4 cut(s) 602, 816, 918, 1151
MwoI GCNNNNNNNGC 5 cut(s) 74, 80, 137, 1009, 1129
NcoI CCATGG 1 cut(s) 363
NdeII GATC 4 cut(s) 240, 847, 1172, 1184
NlaIII CATG 9 cut(s) 74, 314, 367, 410, 690, 694, 767, 916, 1285
NlaIV GGNNCC 2 cut(s) 487, 813
NmeAIII GCCGAG 1 cut(s) 1012
NmuCI GTSAC 3 cut(s) 31, 436, 874
NsiI ATGCAT 1 cut(s) 692
NspI RCATGY 3 cut(s) 690, 694, 767
OliI CACNNNNGTG 1 cut(s) 788
PaeI GCATGC 1 cut(s) 694
PaeR7I CTCGAG 1 cut(s) 724
PceI AGGCCT 1 cut(s) 936
PctI GAATGC 1 cut(s) 735
PfeI GAWTC 4 cut(s) 403, 953, 1154, 1265
PflMI CCANNNNNTGG 1 cut(s) 830
PinAI ACCGGT 2 cut(s) 1134, 1362
PkrI GCNGC 6 cut(s) 76, 133, 577, 735, 837, 1294
PleI GAGTC 1 cut(s) 272
PpsI GAGTC 1 cut(s) 272
PpuMI RGGWCCY 1 cut(s) 811
PsiI TTATAA 1 cut(s) 842
Psp5II RGGWCCY 1 cut(s) 811
Psp6I CCWGG 4 cut(s) 600, 814, 916, 1149
PspGI CCWGG 4 cut(s) 600, 814, 916, 1149
PspN4I GGNNCC 2 cut(s) 487, 813
PspPI GGNCC 6 cut(s) 86, 307, 485, 710, 811, 1346
PspPPI RGGWCCY 1 cut(s) 811
PstI CTGCAG 2 cut(s) 85, 1162
PsuI RGATCY 3 cut(s) 240, 1172, 1184
RsaI GTAC 2 cut(s) 188, 623
RsaNI GTAC 2 cut(s) 187, 622
RseI CAYNNNNRTG 2 cut(s) 315, 788
SaqAI TTAA 4 cut(s) 285, 947, 1107, 1369
SatI GCNGC 6 cut(s) 75, 132, 576, 734, 836, 1293
Sau3AI GATC 4 cut(s) 240, 847, 1172, 1184
Sau96I GGNCC 6 cut(s) 86, 307, 485, 710, 811, 1346
SbfI CCTGCAGG 1 cut(s) 85
SchI GAGTC 1 cut(s) 272
ScrFI CCNGG 4 cut(s) 602, 816, 918, 1151
SdaI CCTGCAGG 1 cut(s) 85
SduI GDGCHC 1 cut(s) 884
SfaNI GCATC 5 cut(s) 143, 322, 487, 954, 1222
SfcI CTRYAG 3 cut(s) 81, 475, 1158
Sfr274I CTCGAG 1 cut(s) 724
SinI GGWCC 4 cut(s) 485, 710, 811, 1346
SlaI CTCGAG 1 cut(s) 724
SmiMI CAYNNNNRTG 2 cut(s) 315, 788
SmlI CTYRAG 1 cut(s) 724
SmoI CTYRAG 1 cut(s) 724
SphI GCATGC 1 cut(s) 694
Sse8387I CCTGCAGG 1 cut(s) 85
Sse9I AATT 5 cut(s) 59, 420, 843, 1163, 1354
SseBI AGGCCT 1 cut(s) 936
SsiI CCGC 3 cut(s) 131, 138, 244
SspI AATATT 1 cut(s) 466
StuI AGGCCT 1 cut(s) 936
StyD4I CCNGG 4 cut(s) 600, 814, 916, 1149
StyI CCWWGG 1 cut(s) 363
TaaI ACNGT 3 cut(s) 43, 202, 626
TaqI TCGA 2 cut(s) 725, 1268
TasI AATT 5 cut(s) 59, 420, 843, 1163, 1354
TatI WGTACW 1 cut(s) 621
TauI GCSGC 1 cut(s) 134
TfiI GAWTC 4 cut(s) 403, 953, 1154, 1265
Tru1I TTAA 4 cut(s) 285, 947, 1107, 1369
Tru9I TTAA 4 cut(s) 285, 947, 1107, 1369
TscAI CASTG 3 cut(s) 496, 795, 879
TseFI GTSAC 3 cut(s) 31, 436, 874
TseI GCWGC 5 cut(s) 74, 575, 733, 835, 1292
Tsp45I GTSAC 3 cut(s) 31, 436, 874
TspDTI ATGAA 7 cut(s) 10, 789, 886, 901, 1091, 1298, 1347
TspGWI ACGGA 1 cut(s) 271
TspRI CASTG 3 cut(s) 496, 795, 879
Van91I CCANNNNNTGG 1 cut(s) 830
VpaK11BI GGWCC 4 cut(s) 485, 710, 811, 1346
XapI RAATTY 1 cut(s) 1163
XceI RCATGY 3 cut(s) 690, 694, 767
XcmI CCANNNNNNNNNTGG 1 cut(s) 790
XhoI CTCGAG 1 cut(s) 724
Zsp2I ATGCAT 1 cut(s) 692
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.