pycom03g19380

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0010007
Physical Location & Seq
Forward (+)
19984289 .. 19989204
4916 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g19380.1

Sequence Viewer

Length: 1446 bp
ATGAGAAATTCCCTTGTCTTCCTCACTCTCCTAACACTCACTGTCACTTACTCCCTTCGATTCCAAGCTCATGCTGCTCCTGCAGGCCCATTGATAAAGCACTTGTCTTCTCTTCTCAAATGGACCAGGGAAATATCCTCCAAAACGCCACAATCAGATGGGAATGTTCTTCAGTTTGAGGATGGGTACTTAGTTGAGACTGTTTTGGAAGGAAATGATATCGGAGTTATTCCTTACAAAATCCGCATCTCAGAGGATGGGGAGCTCTTTGTTGTGGACTCCGTTAATAGCAACATTATGCGGATTACTCCACCATTGTCCCAATATAGTAGGGGAAGATTGGTTGCTGGTTCATTTCAGGGTTACACCGGACATGTTGATGGGAAGCCAAGTGATGCTCGTTTCAATCATCCCAAAGGTGTAACCATGGATGATAAAGGGAATGTGTATGTTGCTGATACCTTGAATCACGCCATAAGAAAGATTGGAGATGCTGGTGTGACAACCATTGCGGGTGGGAAATCAAATGTCGCAGGCTACAGGGATGGGCCCAGTGAAGATGCAATGTTCTCAAATGATTTTGATATCGTATATGTTCAACCTACTTGTTCATTGCTAGTTGTTGACAGAGGAAATGCTGCTCTCCGGCAGATCTCTCTCAACCAGGAGGATTGTGATTTTCAGTACAGTTCAATTTCTGCTACAGATGTCCTTATGGTTGTTGGTGCTGTCTTGGCAGGGTATGCTACATGCATGCTTCAGCAGGGATATGGACCTTCTTTCTTATCGAGAACGCAGCCACCTTCAAAGAGTGAAATGAAAGAACATCCAAGCAGTGAAAAATCCACTCTACTGGTGGAAAGCATGAAAGAGGAACCGGGATGGCCCTCTTTTGGACAGCTCGTCAGTGATCTATCCAAACTTGGACTTGAAGCATTGGCTGGCATATTCGTTCACTTCCTCCCGTCGCGTTTTATGCCTGGTGGCTCCCAGAAAGCCCTCACACCATTAAAAGATTCTCTTAGGATGCCTGAAGACAAATCTGAGGCTCCAGTAGTTCAGAGACAAAGTGCTCCTGCTCCTCTATCTGAAACTCGACAGGCCCATACTCCCATTGCAAGTGAGAAGTATTCAGAAATGAAGCCACCAAAAATGAAGTCAACTAGTTTCAAAGATCCCTCTTTGCCGAGCAAGCACCGGTCTTCAAAACGACAGGATTCTGCAGAGTTCTATGGGTCCGGTGAGGTTTCTTCTCATAGCAGGTCTAAGAGCCAGAAAGAAAGAACAAGGCATCGCCAGCGAGAAAAGACTGGAGAGGTAGTTTATGGAGCTGTTGGGGCAGAAACAAAACCGGTGGATATGAAGCCAGCGGGTTATGACAATCCAAAGTTCGAAAATTACAACATGAGGAGCAGGTATGGGACTGATAGTTCATGCCGCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NHL

Protein Analysis

482

Amino Acids

52.83

Weight (kDa)

8.43

Isoelectric Point (pI)

40.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NHL PF01436 135 - 161 6.1e-06 NHL repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1251, 1404
AccII CGCG 1 cut(s) 970
AciI CCGC 5 cut(s) 244, 301, 512, 1370, 1438
AclWI GGATC 1 cut(s) 1169
AcsI RAATTY 1 cut(s) 7
AcuI CTGAAG 3 cut(s) 155, 743, 1053
AfaI GTAC 2 cut(s) 188, 686
AfiI CCNNNNNNNGG 1 cut(s) 893
AflIII ACRYGT 1 cut(s) 373
AgeI ACCGGT 2 cut(s) 1197, 1351
AgsI TTSAA 8 cut(s) 406, 466, 599, 693, 807, 932, 1171, 1206
AhdI GACNNNNNGTC 1 cut(s) 902
AhlI ACTAGT 1 cut(s) 1163
AjnI CCWGG 3 cut(s) 125, 663, 979
AloI GAACNNNNNNTCC 2 cut(s) 1414, 1446
AluBI AGCT 4 cut(s) 68, 265, 901, 1331
AluI AGCT 4 cut(s) 68, 265, 901, 1331
Alw21I GWGCWC 2 cut(s) 267, 1075
Alw26I GTCTC 2 cut(s) 191, 1057
AlwI GGATC 1 cut(s) 1169
AoxI GGCC 4 cut(s) 85, 548, 884, 1101
ApaI GGGCCC 1 cut(s) 552
ApeKI GCWGC 3 cut(s) 74, 638, 796
ApoI RAATTY 1 cut(s) 7
ArsI GACNNNNNNTTYG 2 cut(s) 1142, 1174
AsiGI ACCGGT 2 cut(s) 1197, 1351
AspS9I GGNCC 8 cut(s) 86, 123, 548, 549, 773, 885, 1102, 1236
AsuC2I CCSGG 1 cut(s) 879
AsuHPI GGTGA 1 cut(s) 1253
AsuII TTCGAA 1 cut(s) 1392
AvaII GGWCC 3 cut(s) 123, 773, 1236
BaeGI GKGCMC 1 cut(s) 552
BanII GRGCYC 2 cut(s) 267, 552
BbsI GAAGAC 4 cut(s) 10, 99, 1041, 1194
Bbv12I GWGCWC 2 cut(s) 267, 1075
BbvI GCAGC 3 cut(s) 61, 625, 808
BccI CCATC 6 cut(s) 152, 176, 251, 374, 539, 876
BciT130I CCWGG 3 cut(s) 127, 665, 981
BcnI CCSGG 1 cut(s) 879
BcoDI GTCTC 2 cut(s) 191, 1057
BcuI ACTAGT 1 cut(s) 1163
BfaI CTAG 2 cut(s) 617, 1164
BfmI CTRYAG 4 cut(s) 81, 538, 702, 1221
BfuAI ACCTGC 2 cut(s) 1251, 1404
BglII AGATCT 1 cut(s) 651
BisI GCNGC 4 cut(s) 75, 639, 797, 1438
BlsI GCNGC 4 cut(s) 76, 640, 798, 1439
Bme1390I CCNGG 4 cut(s) 127, 665, 879, 981
Bme18I GGWCC 3 cut(s) 123, 773, 1236
BmeRI GACNNNNNGTC 1 cut(s) 902
BmgT120I GGNCC 8 cut(s) 86, 123, 548, 549, 773, 885, 1102, 1236
BmiI GGNNCC 5 cut(s) 550, 876, 988, 1050, 1237
BmrFI CCNGG 4 cut(s) 127, 665, 879, 981
BmrI ACTGGG 1 cut(s) 546
BmsI GCATC 6 cut(s) 255, 385, 481, 550, 1017, 1300
BmuI ACTGGG 1 cut(s) 546
BpiI GAAGAC 4 cut(s) 10, 99, 1041, 1194
BpmI CTGGAG 2 cut(s) 1035, 1332
Bpu14I TTCGAA 1 cut(s) 1392
BpuMI CCSGG 1 cut(s) 879
BsaJI CCNNGG 2 cut(s) 126, 426
BsaWI WCCGGW 4 cut(s) 368, 1197, 1238, 1351
BsaXI ACNNNNNCTCC 2 cut(s) 1305, 1335
Bsc4I CCNNNNNNNGG 1 cut(s) 893
Bse118I RCCGGY 2 cut(s) 1197, 1351
Bse1I ACTGG 4 cut(s) 552, 858, 1052, 1315
Bse3DI GCAATG 4 cut(s) 507, 570, 611, 1113
BseBI CCWGG 3 cut(s) 127, 665, 981
BseDI CCNNGG 2 cut(s) 126, 426
BseGI GGATG 8 cut(s) 187, 262, 409, 436, 550, 826, 887, 1032
BseLI CCNNNNNNNGG 1 cut(s) 893
BseMI GCAATG 4 cut(s) 507, 570, 611, 1113
BseMII CTCAG 2 cut(s) 264, 1035
BseNI ACTGG 4 cut(s) 552, 858, 1052, 1315
BseRI GAGGAG 2 cut(s) 1071, 1423
BseSI GKGCMC 1 cut(s) 552
BseXI GCAGC 3 cut(s) 61, 625, 808
Bsh1236I CGCG 1 cut(s) 970
BshFI GGCC 4 cut(s) 87, 550, 886, 1103
BshTI ACCGGT 2 cut(s) 1197, 1351
BsiHKAI GWGCWC 2 cut(s) 267, 1075
BsiSI CCGG 6 cut(s) 369, 646, 878, 1198, 1239, 1352
BslFI GGGAC 2 cut(s) 304, 1435
BslI CCNNNNNNNGG 1 cut(s) 893
BsmAI GTCTC 2 cut(s) 191, 1057
BsmFI GGGAC 2 cut(s) 304, 1435
BsnI GGCC 4 cut(s) 87, 550, 886, 1103
Bsp119I TTCGAA 1 cut(s) 1392
Bsp120I GGGCCC 1 cut(s) 548
Bsp1286I GDGCHC 3 cut(s) 267, 552, 1075
Bsp143I GATC 3 cut(s) 651, 910, 1174
Bsp19I CCATGG 1 cut(s) 426
BspACI CCGC 5 cut(s) 244, 301, 512, 1370, 1438
BspANI GGCC 4 cut(s) 87, 550, 886, 1103
BspCNI CTCAG 2 cut(s) 263, 1036
BspFNI CGCG 1 cut(s) 970
BspLI GGNNCC 5 cut(s) 550, 876, 988, 1050, 1237
BspMAI CTGCAG 2 cut(s) 85, 1225
BspMI ACCTGC 2 cut(s) 1251, 1404
BspPI GGATC 1 cut(s) 1169
BspT104I TTCGAA 1 cut(s) 1392
BsrDI GCAATG 4 cut(s) 507, 570, 611, 1113
BsrFI RCCGGY 2 cut(s) 1197, 1351
BsrI ACTGG 4 cut(s) 552, 858, 1052, 1315
BssAI RCCGGY 2 cut(s) 1197, 1351
BssECI CCNNGG 2 cut(s) 126, 426
BssMI GATC 3 cut(s) 651, 910, 1174
BssT1I CCWWGG 1 cut(s) 426
Bst2UI CCWGG 3 cut(s) 127, 665, 981
Bst4CI ACNGT 3 cut(s) 43, 202, 689
Bst6I CTCTTC 1 cut(s) 117
BstAPI GCANNNNNTGC 1 cut(s) 743
BstBI TTCGAA 1 cut(s) 1392
BstC8I GCNNGC 7 cut(s) 85, 535, 755, 943, 1193, 1298, 1368
BstDEI CTNAG 5 cut(s) 190, 250, 1022, 1044, 1266
BstDSI CCRYGG 1 cut(s) 426
BstF5I GGATG 8 cut(s) 187, 262, 409, 436, 550, 826, 887, 1032
BstFNI CGCG 1 cut(s) 970
BstKTI GATC 3 cut(s) 654, 913, 1177
BstMAI GTCTC 2 cut(s) 191, 1057
BstMBI GATC 3 cut(s) 651, 910, 1174
BstMWI GCNNNNNNNGC 8 cut(s) 74, 80, 734, 743, 976, 1192, 1297, 1337
BstNI CCWGG 3 cut(s) 127, 665, 981
BstNSI RCATGY 3 cut(s) 377, 753, 757
BstSCI CCNGG 4 cut(s) 125, 663, 877, 979
BstSFI CTRYAG 4 cut(s) 81, 538, 702, 1221
BstSLI GKGCMC 1 cut(s) 552
BstUI CGCG 1 cut(s) 970
BstV1I GCAGC 3 cut(s) 61, 625, 808
BstV2I GAAGAC 4 cut(s) 10, 99, 1041, 1194
BstX2I RGATCY 2 cut(s) 651, 1174
BstXI CCANNNNNNTGG 1 cut(s) 853
BstYI RGATCY 2 cut(s) 651, 1174
BsuRI GGCC 4 cut(s) 87, 550, 886, 1103
BtgI CCRYGG 1 cut(s) 426
BtgZI GCGATG 1 cut(s) 1277
BtsCI GGATG 8 cut(s) 187, 262, 409, 436, 550, 826, 887, 1032
BtsI GCAGTG 1 cut(s) 841
BtsIMutI CAGTG 4 cut(s) 39, 559, 841, 913
BveI ACCTGC 2 cut(s) 1251, 1404
Cac8I GCNNGC 7 cut(s) 85, 535, 755, 943, 1193, 1298, 1368
Cfr10I RCCGGY 2 cut(s) 1197, 1351
Cfr13I GGNCC 8 cut(s) 86, 123, 548, 549, 773, 885, 1102, 1236
Csp6I GTAC 2 cut(s) 187, 685
CspAI ACCGGT 2 cut(s) 1197, 1351
CspCI CAANNNNNGTGG 2 cut(s) 1335, 1370
CviAII CATG 8 cut(s) 71, 374, 427, 750, 754, 865, 1405, 1434
CviQI GTAC 2 cut(s) 187, 685
DdeI CTNAG 5 cut(s) 190, 250, 1022, 1044, 1266
DpnI GATC 3 cut(s) 653, 912, 1176
DpnII GATC 3 cut(s) 651, 910, 1174
DriI GACNNNNNGTC 1 cut(s) 902
Eam1104I CTCTTC 1 cut(s) 117
Eam1105I GACNNNNNGTC 1 cut(s) 902
EarI CTCTTC 1 cut(s) 117
Ecl136II GAGCTC 1 cut(s) 265
Eco130I CCWWGG 1 cut(s) 426
Eco24I GRGCYC 2 cut(s) 267, 552
Eco32I GATATC 2 cut(s) 220, 586
Eco47I GGWCC 3 cut(s) 123, 773, 1236
Eco53kI GAGCTC 1 cut(s) 265
Eco57I CTGAAG 3 cut(s) 155, 743, 1053
EcoICRI GAGCTC 1 cut(s) 265
EcoRII CCWGG 3 cut(s) 125, 663, 979
EcoRV GATATC 2 cut(s) 220, 586
EcoT14I CCWWGG 1 cut(s) 426
EcoT22I ATGCAT 1 cut(s) 755
EcoT38I GRGCYC 2 cut(s) 267, 552
ErhI CCWWGG 1 cut(s) 426
FaeI CATG 8 cut(s) 74, 377, 430, 753, 757, 868, 1408, 1437
FalI AAGNNNNNCTT 2 cut(s) 1005, 1037
FaqI GGGAC 2 cut(s) 304, 1435
FatI CATG 8 cut(s) 70, 373, 426, 749, 753, 864, 1404, 1433
FauI CCCGC 2 cut(s) 505, 1363
Fnu4HI GCNGC 4 cut(s) 75, 639, 797, 1438
FokI GGATG 8 cut(s) 194, 269, 396, 443, 557, 813, 894, 1039
FriOI GRGCYC 2 cut(s) 267, 552
Fsp4HI GCNGC 4 cut(s) 75, 639, 797, 1438
FspBI CTAG 2 cut(s) 617, 1164
GluI GCNGC 4 cut(s) 75, 639, 797, 1438
GsuI CTGGAG 2 cut(s) 1035, 1332
HaeIII GGCC 4 cut(s) 87, 550, 886, 1103
HapII CCGG 6 cut(s) 369, 646, 878, 1198, 1239, 1352
Hin1II CATG 8 cut(s) 74, 377, 430, 753, 757, 868, 1408, 1437
HincII GTYRAC 2 cut(s) 625, 1161
HindII GTYRAC 2 cut(s) 625, 1161
HinfI GANTC 5 cut(s) 60, 278, 466, 1016, 1217
HpaII CCGG 6 cut(s) 369, 646, 878, 1198, 1239, 1352
HphI GGTGA 1 cut(s) 1253
Hpy166II GTNNAC 4 cut(s) 277, 625, 955, 1161
Hpy188I TCNGA 7 cut(s) 157, 224, 253, 1045, 1062, 1090, 1135
Hpy188III TCNNGA 1 cut(s) 789
Hpy8I GTNNAC 4 cut(s) 277, 625, 955, 1161
Hpy99I CGWCG 1 cut(s) 970
HpyAV CCTTC 4 cut(s) 65, 203, 786, 813
HpyCH4III ACNGT 3 cut(s) 43, 202, 689
HpyCH4V TGCA 5 cut(s) 83, 563, 753, 1118, 1223
HpyF10VI GCNNNNNNNGC 8 cut(s) 74, 80, 734, 743, 976, 1192, 1297, 1337
HpyF3I CTNAG 5 cut(s) 190, 250, 1022, 1044, 1266
Hsp92II CATG 8 cut(s) 74, 377, 430, 753, 757, 868, 1408, 1437
Kzo9I GATC 3 cut(s) 651, 910, 1174
LmnI GCTCC 8 cut(s) 82, 262, 992, 1054, 1078, 1084, 1328, 1410
Lsp1109I GCAGC 3 cut(s) 61, 625, 808
LweI GCATC 6 cut(s) 255, 385, 481, 550, 1017, 1300
MaeI CTAG 2 cut(s) 617, 1164
MaeIII GTNAC 4 cut(s) 43, 362, 421, 499
MalI GATC 3 cut(s) 653, 912, 1176
MboI GATC 3 cut(s) 651, 910, 1174
MboII GAAGA 9 cut(s) 10, 99, 104, 161, 348, 569, 1046, 1194, 1242
MflI RGATCY 2 cut(s) 651, 1174
MhlI GDGCHC 3 cut(s) 267, 552, 1075
MluCI AATT 3 cut(s) 7, 693, 1396
MlyI GAGTC 1 cut(s) 272
Mph1103I ATGCAT 1 cut(s) 755
MseI TTAA 2 cut(s) 285, 1010
MslI CAYNNNNRTG 1 cut(s) 378
MspA1I CMGCKG 1 cut(s) 1370
MspI CCGG 6 cut(s) 369, 646, 878, 1198, 1239, 1352
MspR9I CCNGG 4 cut(s) 127, 665, 879, 981
MvaI CCWGG 3 cut(s) 127, 665, 981
MvnI CGCG 1 cut(s) 970
MwoI GCNNNNNNNGC 8 cut(s) 74, 80, 734, 743, 976, 1192, 1297, 1337
NciI CCSGG 1 cut(s) 879
NcoI CCATGG 1 cut(s) 426
NdeII GATC 3 cut(s) 651, 910, 1174
NlaIII CATG 8 cut(s) 74, 377, 430, 753, 757, 868, 1408, 1437
NlaIV GGNNCC 5 cut(s) 550, 876, 988, 1050, 1237
NmeAIII GCCGAG 1 cut(s) 1212
NmuCI GTSAC 2 cut(s) 43, 499
NsiI ATGCAT 1 cut(s) 755
NspI RCATGY 3 cut(s) 377, 753, 757
NspV TTCGAA 1 cut(s) 1392
PaeI GCATGC 1 cut(s) 757
PciI ACATGT 1 cut(s) 373
PfeI GAWTC 4 cut(s) 60, 466, 1016, 1217
PinAI ACCGGT 2 cut(s) 1197, 1351
PkrI GCNGC 4 cut(s) 76, 640, 798, 1439
PleI GAGTC 1 cut(s) 272
PpsI GAGTC 1 cut(s) 272
PscI ACATGT 1 cut(s) 373
Psp124BI GAGCTC 1 cut(s) 267
Psp6I CCWGG 3 cut(s) 125, 663, 979
PspGI CCWGG 3 cut(s) 125, 663, 979
PspN4I GGNNCC 5 cut(s) 550, 876, 988, 1050, 1237
PspOMI GGGCCC 1 cut(s) 548
PspPI GGNCC 8 cut(s) 86, 123, 548, 549, 773, 885, 1102, 1236
PstI CTGCAG 2 cut(s) 85, 1225
PsuI RGATCY 2 cut(s) 651, 1174
RsaI GTAC 2 cut(s) 188, 686
RsaNI GTAC 2 cut(s) 187, 685
RseI CAYNNNNRTG 1 cut(s) 378
SacI GAGCTC 1 cut(s) 267
SaqAI TTAA 2 cut(s) 285, 1010
SatI GCNGC 4 cut(s) 75, 639, 797, 1438
Sau3AI GATC 3 cut(s) 651, 910, 1174
Sau96I GGNCC 8 cut(s) 86, 123, 548, 549, 773, 885, 1102, 1236
SbfI CCTGCAGG 1 cut(s) 85
SchI GAGTC 1 cut(s) 272
ScrFI CCNGG 4 cut(s) 127, 665, 879, 981
SdaI CCTGCAGG 1 cut(s) 85
SduI GDGCHC 3 cut(s) 267, 552, 1075
SfaNI GCATC 6 cut(s) 255, 385, 481, 550, 1017, 1300
SfcI CTRYAG 4 cut(s) 81, 538, 702, 1221
SfuI TTCGAA 1 cut(s) 1392
SinI GGWCC 3 cut(s) 123, 773, 1236
SmiMI CAYNNNNRTG 1 cut(s) 378
SpeI ACTAGT 1 cut(s) 1163
SphI GCATGC 1 cut(s) 757
Sse8387I CCTGCAGG 1 cut(s) 85
Sse9I AATT 3 cut(s) 7, 693, 1396
SsiI CCGC 5 cut(s) 244, 301, 512, 1370, 1438
SspMI CTAG 2 cut(s) 617, 1164
SstI GAGCTC 1 cut(s) 267
StyD4I CCNGG 4 cut(s) 125, 663, 877, 979
StyI CCWWGG 1 cut(s) 426
TaaI ACNGT 3 cut(s) 43, 202, 689
TaqI TCGA 4 cut(s) 58, 788, 1096, 1392
TasI AATT 3 cut(s) 7, 693, 1396
TatI WGTACW 1 cut(s) 684
TauI GCSGC 1 cut(s) 1440
TfiI GAWTC 4 cut(s) 60, 466, 1016, 1217
Tru1I TTAA 2 cut(s) 285, 1010
Tru9I TTAA 2 cut(s) 285, 1010
TscAI CASTG 4 cut(s) 46, 559, 841, 913
TseFI GTSAC 2 cut(s) 43, 499
TseI GCWGC 3 cut(s) 74, 638, 796
Tsp45I GTSAC 2 cut(s) 43, 499
TspDTI ATGAA 8 cut(s) 342, 600, 833, 881, 1154, 1169, 1376, 1422
TspGWI ACGGA 1 cut(s) 271
TspRI CASTG 4 cut(s) 46, 559, 841, 913
VpaK11BI GGWCC 3 cut(s) 123, 773, 1236
XapI RAATTY 1 cut(s) 7
XceI RCATGY 3 cut(s) 377, 753, 757
XcmI CCANNNNNNNNNTGG 1 cut(s) 853
XspI CTAG 2 cut(s) 617, 1164
Zsp2I ATGCAT 1 cut(s) 755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.