Rorug05G0118400

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0010007
Physical Location & Seq
Forward (+)
10460998 .. 10464091
3094 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0118400.1

Sequence Viewer

Length: 951 bp
ATGAATAATCATTTTGGGATTTGCAGGTTTCAGGAATGTTGGGTTGAAGAATCTCAGAGGTCCAAGCCATGGCTTCTGAGAGCACTGCATTGTAGCCTTGGACGAAGGTTCTGGTTGGGAGGCTTTTTCAAGATTGGCCAGGATCTGTCTCAGTTATCGAGTCCCATTCTATTAAATCATCTCTTGCAGTCAATGCAGCGAGGTGATCCAGCTTGGATTGGTTATATATATGCCTTCTTAATTTTCATTGGGGTGTCATTTGGTGTGCTCCCTGCATCGCAGTGTTACCAGAATCTTCAGCGAGTCGGCTTTCGGTTGAGATCAACTTTGGTAGCTGCTATATTCCGTAAATCTATAAGAATAACACATGAGGGCCGTAAGAAGTTCCCAACTGGGAAGATAACAAATATGATATCTACAGATGCTGATGCACTCCAGCAAATAGCCCGAAACCTTCACGGACTATGGTCTGCTCCTTTTCGTATCATTGTAGCTATGGTTCTTCTTTACCAACAATTAGGTGTTGCATCGCTTATTGGATCCTTGATGCTAGTTCTCGCAATCCCCATCCAGACAACGATGTTCAGCAAAATGCGGAAACTGACAAAGGATGGACTGCAACAGACTGACAAGAGAGTTAGCCTCATGAATGAAATTTTAGCTGCCATGGACACTGTGAAATGTTATTCCTGGGAGACAAGTTTCCAGCAGCAAGTTCAAAATATACGAAATGATGAGCTTTCAAGGTTCTGTAAAGCGCAATTACTATCTGCTTTGAACAGTTTTATACTCAACAGCATCCCAGCTGTTGTGACAGTAACTTCATTTGGGGCGTTCACTTTCCTTGGAGGCGAATTGACACCTGCAAGGGCATTTACATCGCTTTCTCTTCTTGCAGTGCTACGCTTCCCTTTAGAAATGCTGCCCAACTTATTAATTCAGGTAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NHL

Protein Analysis

316

Amino Acids

35.64

Weight (kDa)

9.99

Isoelectric Point (pI)

42.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 38 - 305 2.5e-37 ABC transporter transmembrane region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 871
Acc36I ACCTGC 2 cut(s) 15, 871
AccB7I CCANNNNNTGG 1 cut(s) 69
AciI CCGC 1 cut(s) 595
AclWI GGATC 4 cut(s) 150, 200, 534, 547
AcoI YGGCCR 1 cut(s) 136
AcsI RAATTY 1 cut(s) 654
AcuI CTGAAG 1 cut(s) 281
AfiI CCNNNNNNNGG 1 cut(s) 69
AgsI TTSAA 5 cut(s) 47, 130, 719, 744, 778
AjnI CCWGG 2 cut(s) 138, 689
AluBI AGCT 6 cut(s) 212, 335, 494, 662, 739, 806
AluI AGCT 6 cut(s) 212, 335, 494, 662, 739, 806
Alw21I GWGCWC 2 cut(s) 85, 270
Alw26I GTCTC 2 cut(s) 153, 689
AlwI GGATC 4 cut(s) 150, 200, 534, 547
AlwNI CAGNNNCTG 2 cut(s) 145, 425
AoxI GGCC 2 cut(s) 136, 373
ApeKI GCWGC 5 cut(s) 196, 335, 662, 709, 922
ApoI RAATTY 1 cut(s) 654
AseI ATTAAT 1 cut(s) 935
AspLEI GCGC 1 cut(s) 760
AspS9I GGNCC 2 cut(s) 60, 373
AsuHPI GGTGA 1 cut(s) 215
AvaII GGWCC 1 cut(s) 60
BalI TGGCCA 1 cut(s) 138
BamHI GGATCC 1 cut(s) 539
Bbv12I GWGCWC 2 cut(s) 85, 270
BbvI GCAGC 5 cut(s) 208, 322, 649, 721, 909
BccI CCATC 2 cut(s) 575, 605
BceAI ACGGC 1 cut(s) 360
BcgI CGANNNNNNTGC 2 cut(s) 861, 895
BciT130I CCWGG 2 cut(s) 140, 691
BcoDI GTCTC 2 cut(s) 153, 689
BfaI CTAG 1 cut(s) 551
BfmI CTRYAG 1 cut(s) 417
BfuAI ACCTGC 2 cut(s) 15, 871
BisI GCNGC 5 cut(s) 197, 336, 663, 710, 923
BlsI GCNGC 5 cut(s) 198, 337, 664, 711, 924
Bme1390I CCNGG 2 cut(s) 140, 691
Bme18I GGWCC 1 cut(s) 60
BmgT120I GGNCC 2 cut(s) 60, 373
BmiI GGNNCC 1 cut(s) 541
BmrFI CCNGG 2 cut(s) 140, 691
BmrI ACTGGG 1 cut(s) 402
BmsI GCATC 6 cut(s) 284, 412, 418, 536, 537, 807
BmuI ACTGGG 1 cut(s) 402
BoxI GACNNNNGTC 1 cut(s) 466
BplI GAGNNNNNCTC 2 cut(s) 627, 659
BpmI CTGGAG 1 cut(s) 419
BsaJI CCNNGG 5 cut(s) 68, 97, 666, 690, 844
Bsc4I CCNNNNNNNGG 1 cut(s) 69
Bse1I ACTGG 1 cut(s) 397
BseBI CCWGG 2 cut(s) 140, 691
BseDI CCNNGG 5 cut(s) 68, 97, 666, 690, 844
BseGI GGATG 3 cut(s) 567, 616, 798
BseLI CCNNNNNNNGG 1 cut(s) 69
BseMII CTCAG 3 cut(s) 68, 68, 164
BseNI ACTGG 1 cut(s) 397
BseXI GCAGC 5 cut(s) 208, 322, 649, 721, 909
BseYI CCCAGC 1 cut(s) 802
BshFI GGCC 2 cut(s) 138, 375
BsiHKAI GWGCWC 2 cut(s) 85, 270
BslFI GGGAC 1 cut(s) 147
BslI CCNNNNNNNGG 1 cut(s) 69
BsmAI GTCTC 2 cut(s) 153, 689
BsmFI GGGAC 1 cut(s) 147
BsnI GGCC 2 cut(s) 138, 375
Bsp1286I GDGCHC 2 cut(s) 85, 270
Bsp143I GATC 4 cut(s) 142, 205, 320, 539
Bsp19I CCATGG 2 cut(s) 68, 666
BspACI CCGC 1 cut(s) 595
BspANI GGCC 2 cut(s) 138, 375
BspCNI CTCAG 3 cut(s) 67, 69, 163
BspHI TCATGA 1 cut(s) 645
BspLI GGNNCC 1 cut(s) 541
BspMI ACCTGC 2 cut(s) 15, 871
BspPI GGATC 4 cut(s) 150, 200, 534, 547
BsrI ACTGG 1 cut(s) 397
BssECI CCNNGG 5 cut(s) 68, 97, 666, 690, 844
BssMI GATC 4 cut(s) 142, 205, 320, 539
BssT1I CCWWGG 4 cut(s) 68, 97, 666, 844
Bst2UI CCWGG 2 cut(s) 140, 691
Bst4CI ACNGT 3 cut(s) 676, 782, 817
Bst6I CTCTTC 1 cut(s) 894
BstAPI GCANNNNNTGC 1 cut(s) 193
BstDEI CTNAG 3 cut(s) 54, 77, 150
BstDSI CCRYGG 2 cut(s) 68, 666
BstF5I GGATG 3 cut(s) 567, 616, 798
BstHHI GCGC 1 cut(s) 760
BstKTI GATC 4 cut(s) 145, 208, 323, 542
BstMAI GTCTC 2 cut(s) 153, 689
BstMBI GATC 4 cut(s) 142, 205, 320, 539
BstMWI GCNNNNNNNGC 1 cut(s) 193
BstNI CCWGG 2 cut(s) 140, 691
BstPAI GACNNNNGTC 1 cut(s) 466
BstSCI CCNGG 2 cut(s) 138, 689
BstSFI CTRYAG 1 cut(s) 417
BstV1I GCAGC 5 cut(s) 208, 322, 649, 721, 909
BstX2I RGATCY 2 cut(s) 142, 539
BstYI RGATCY 2 cut(s) 142, 539
BsuRI GGCC 2 cut(s) 138, 375
BtgI CCRYGG 2 cut(s) 68, 666
BtgZI GCGATG 3 cut(s) 261, 513, 864
BtsCI GGATG 3 cut(s) 567, 616, 798
BtsI GCAGTG 3 cut(s) 83, 287, 903
BtsIMutI CAGTG 4 cut(s) 83, 287, 672, 903
BveI ACCTGC 2 cut(s) 15, 871
CaiI CAGNNNCTG 2 cut(s) 145, 425
CciI TCATGA 1 cut(s) 645
CfoI GCGC 1 cut(s) 760
Cfr13I GGNCC 2 cut(s) 60, 373
CviAII CATG 5 cut(s) 69, 368, 646, 667, 948
DdeI CTNAG 3 cut(s) 54, 77, 150
DpnI GATC 4 cut(s) 144, 207, 322, 541
DpnII GATC 4 cut(s) 142, 205, 320, 539
EaeI YGGCCR 1 cut(s) 136
Eam1104I CTCTTC 1 cut(s) 894
EarI CTCTTC 1 cut(s) 894
Eco130I CCWWGG 4 cut(s) 68, 97, 666, 844
Eco32I GATATC 1 cut(s) 414
Eco47I GGWCC 1 cut(s) 60
Eco57I CTGAAG 1 cut(s) 281
EcoRII CCWGG 2 cut(s) 138, 689
EcoRV GATATC 1 cut(s) 414
EcoT14I CCWWGG 4 cut(s) 68, 97, 666, 844
ErhI CCWWGG 4 cut(s) 68, 97, 666, 844
FaeI CATG 5 cut(s) 72, 371, 649, 670, 951
FaqI GGGAC 1 cut(s) 147
FatI CATG 5 cut(s) 68, 367, 645, 666, 947
Fnu4HI GCNGC 5 cut(s) 197, 336, 663, 710, 923
FokI GGATG 3 cut(s) 554, 623, 785
Fsp4HI GCNGC 5 cut(s) 197, 336, 663, 710, 923
FspBI CTAG 1 cut(s) 551
GlaI GCGC 1 cut(s) 759
GluI GCNGC 5 cut(s) 197, 336, 663, 710, 923
GsaI CCCAGC 1 cut(s) 806
GsuI CTGGAG 1 cut(s) 419
HaeIII GGCC 2 cut(s) 138, 375
HhaI GCGC 1 cut(s) 760
Hin1II CATG 5 cut(s) 72, 371, 649, 670, 951
Hin6I GCGC 1 cut(s) 758
HinP1I GCGC 1 cut(s) 758
HinfI GANTC 4 cut(s) 50, 160, 292, 303
HphI GGTGA 1 cut(s) 215
Hpy166II GTNNAC 1 cut(s) 837
Hpy188I TCNGA 2 cut(s) 57, 78
Hpy188III TCNNGA 4 cut(s) 32, 130, 571, 646
Hpy8I GTNNAC 1 cut(s) 837
HpyAV CCTTC 3 cut(s) 99, 244, 464
HpyCH4III ACNGT 3 cut(s) 676, 782, 817
HpyF10VI GCNNNNNNNGC 1 cut(s) 193
HpyF3I CTNAG 3 cut(s) 54, 77, 150
Hsp92II CATG 5 cut(s) 72, 371, 649, 670, 951
HspAI GCGC 1 cut(s) 758
Kzo9I GATC 4 cut(s) 142, 205, 320, 539
LmnI GCTCC 2 cut(s) 273, 478
Lsp1109I GCAGC 5 cut(s) 208, 322, 649, 721, 909
LweI GCATC 6 cut(s) 284, 412, 418, 536, 537, 807
MaeI CTAG 1 cut(s) 551
MaeIII GTNAC 3 cut(s) 284, 811, 817
MalI GATC 4 cut(s) 144, 207, 322, 541
MboI GATC 4 cut(s) 142, 205, 320, 539
MboII GAAGA 5 cut(s) 59, 287, 409, 494, 881
MflI RGATCY 2 cut(s) 142, 539
MhlI GDGCHC 2 cut(s) 85, 270
MlsI TGGCCA 1 cut(s) 138
MluCI AATT 6 cut(s) 240, 515, 654, 761, 854, 936
MluNI TGGCCA 1 cut(s) 138
MlyI GAGTC 2 cut(s) 169, 312
MnlI CCTC 6 cut(s) 51, 113, 194, 364, 653, 842
Mox20I TGGCCA 1 cut(s) 138
MscI TGGCCA 1 cut(s) 138
MseI TTAA 3 cut(s) 173, 239, 935
MslI CAYNNNNRTG 2 cut(s) 251, 280
Msp20I TGGCCA 1 cut(s) 138
MspA1I CMGCKG 1 cut(s) 806
MspR9I CCNGG 2 cut(s) 140, 691
MvaI CCWGG 2 cut(s) 140, 691
MwoI GCNNNNNNNGC 1 cut(s) 193
NcoI CCATGG 2 cut(s) 68, 666
NdeII GATC 4 cut(s) 142, 205, 320, 539
NlaIII CATG 5 cut(s) 72, 371, 649, 670, 951
NlaIV GGNNCC 1 cut(s) 541
NmuCI GTSAC 1 cut(s) 811
PagI TCATGA 1 cut(s) 645
PaqCI CACCTGC 1 cut(s) 871
PfeI GAWTC 2 cut(s) 50, 292
PflMI CCANNNNNTGG 1 cut(s) 69
PkrI GCNGC 5 cut(s) 198, 337, 664, 711, 924
PleI GAGTC 2 cut(s) 168, 311
PpsI GAGTC 2 cut(s) 168, 311
PshAI GACNNNNGTC 1 cut(s) 466
PshBI ATTAAT 1 cut(s) 935
Psp6I CCWGG 2 cut(s) 138, 689
PspFI CCCAGC 1 cut(s) 802
PspGI CCWGG 2 cut(s) 138, 689
PspN4I GGNNCC 1 cut(s) 541
PspPI GGNCC 2 cut(s) 60, 373
PsrI GAACNNNNNNTAC 2 cut(s) 483, 515
PstNI CAGNNNCTG 2 cut(s) 145, 425
PsuI RGATCY 2 cut(s) 142, 539
PvuII CAGCTG 1 cut(s) 806
RseI CAYNNNNRTG 2 cut(s) 251, 280
SaqAI TTAA 3 cut(s) 173, 239, 935
SatI GCNGC 5 cut(s) 197, 336, 663, 710, 923
Sau3AI GATC 4 cut(s) 142, 205, 320, 539
Sau96I GGNCC 2 cut(s) 60, 373
SchI GAGTC 2 cut(s) 169, 312
ScrFI CCNGG 2 cut(s) 140, 691
SduI GDGCHC 2 cut(s) 85, 270
SfaNI GCATC 6 cut(s) 284, 412, 418, 536, 537, 807
SfcI CTRYAG 1 cut(s) 417
SinI GGWCC 1 cut(s) 60
SmiMI CAYNNNNRTG 2 cut(s) 251, 280
Sse9I AATT 6 cut(s) 240, 515, 654, 761, 854, 936
SsiI CCGC 1 cut(s) 595
SspMI CTAG 1 cut(s) 551
StyD4I CCNGG 2 cut(s) 138, 689
StyI CCWWGG 4 cut(s) 68, 97, 666, 844
TaaI ACNGT 3 cut(s) 676, 782, 817
TaqI TCGA 1 cut(s) 158
TasI AATT 6 cut(s) 240, 515, 654, 761, 854, 936
TfiI GAWTC 2 cut(s) 50, 292
Tru1I TTAA 3 cut(s) 173, 239, 935
Tru9I TTAA 3 cut(s) 173, 239, 935
TscAI CASTG 4 cut(s) 90, 287, 679, 903
TseFI GTSAC 1 cut(s) 811
TseI GCWGC 5 cut(s) 196, 335, 662, 709, 922
Tsp45I GTSAC 1 cut(s) 811
TspDTI ATGAA 5 cut(s) 17, 235, 662, 666, 813
TspGWI ACGGA 2 cut(s) 335, 474
TspRI CASTG 4 cut(s) 90, 287, 679, 903
Van91I CCANNNNNTGG 1 cut(s) 69
VpaK11BI GGWCC 1 cut(s) 60
VspI ATTAAT 1 cut(s) 935
XapI RAATTY 1 cut(s) 654
XspI CTAG 1 cut(s) 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.