RchiOBHm_Chr5g0030571

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0010007
Physical Location & Seq
Reverse (-)
24188877 .. 24194021
5145 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30989

Sequence Viewer

Length: 1428 bp
ATGACAAGTTCCCTTTTCTTCATCACCATCGTCACTCTCACAGTCGCTTACACCCTCCAATTCAAAGCCCATGCTGCTCCTGCAGGGCCATTGATAAAGCACTTGTCTTCCCTTGTCAAGTGGACGAGGGCGGCATCCGCCAAAGCACCCCAATCAGATGGGAATGTTGTTCAGTTTGAGAGTGGGTACTTAGTTGAGACTGTTGTGGAAGGAACTGATATTGGAGTTGTTCCTTTCAAGATCCGCATTTCAGAGGATGGCGAACTCTTTGCTGTGGACTCCGTTAATAGCAACATTGTTCGGATTACTCCTCCGCTGTCCCAATATAGTAGGGGAAGATTGGTTGCTGGATCATTTCAAGGTTATACAGGCCATGTTGATGGAAAGCCAAGTGATGCTCGTTTCAATCATCCAAAAGGTGTAACCATGGATGATAAAGGGAATGTATATGTTGCTGATACCTTGAATCACGCCATAAGAAAAATTGGAGAAGCTGGTGTGACAACCATAGCTGGTGGAAAATCAAATATTGCTGGCTACAGGGATGGACCCAGTGAAGATGCAATGTTCTCAAACGATTTTGATGTAGTATATGTCCAGCCTACTTGTTCCTTGCTTGTTGTTGATAGAGGAAATGCTGCTCTTAGGCAGATATCTCTCAACCAGGAGGATTGTGATTATCAGTACAGTTCAATCTCTTCCACAGATGTCCTTATGGTTGTTGGTGCTGTCTTGATAGGCTATGCCACATGCATGCTTCAGCAGGGTTTTGGACCTTTCTTCTCGAGAACGCAACCTTCAGAGAGTGAAACTAAAGAACATGCAAGCAATGAAAAATCCACTCCAGTTGTGGAGATTGTGAAAGAGGACCCAGGATGGCCATCTTTTGGGCAGCTTATAATTGATCTATGCAAGATTGGACTTGAAGCAGTGACAGGCACATTTCTTCAGTTCATTCCCTCTCGGTTCATGCCTGGTGGTTCTCACAAAGGCCTGACACCATTAAAAGATTCTCTCAGGATGCCTGAGGAGGAAGCTGAGACCCCGTTAGTTCACAGGCAAAGCACTCCTGCTCCTCTGTCTGAAACTCGGCAGTCCCATACTCCCAATGCAAGTGATAGATATTCAGATATGAAGCCCTCAAAGATAAAGTCAAGTAGCTTTAAAGACCCCTCTTTGCCAAGCAAGCACCGGTCTTCAAAACGCCAGGATTCTGCAGAATTCTATGGATCTGGTGAAAGATCTAAGAGCCAGAAAGAAAGAACACGGCATCGCCAACGAGAGAAGAGTGGAGAGGTAGTTTTTGGGGCCGTTGGGACAGAATCGAAACCTGTGGACATGAAGACAGCAGCAGGTTATGACAATCAGAAGTTTGAGAACTACAATATGAGGAGCAGGTATGGACCTGACAAGTCATACCGGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NHL

Protein Analysis

475

Amino Acids

52.07

Weight (kDa)

7.69

Isoelectric Point (pI)

38.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NHL PF01436 135 - 161 6e-06 NHL repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 899
Acc36I ACCTGC 2 cut(s) 1343, 1386
AccB7I CCANNNNNTGG 1 cut(s) 887
AciI CCGC 4 cut(s) 131, 138, 244, 314
AclWI GGATC 3 cut(s) 235, 358, 1237
AcoI YGGCCR 1 cut(s) 878
AcsI RAATTY 1 cut(s) 1220
AcuI CTGAAG 3 cut(s) 743, 783, 932
AfaI GTAC 2 cut(s) 188, 686
AfiI CCNNNNNNNGG 1 cut(s) 887
AgeI ACCGGT 2 cut(s) 1191, 1419
AgsI TTSAA 8 cut(s) 64, 238, 359, 406, 466, 693, 926, 1200
AjnI CCWGG 4 cut(s) 663, 871, 973, 1206
AluBI AGCT 5 cut(s) 494, 512, 895, 1037, 1161
AluI AGCT 5 cut(s) 494, 512, 895, 1037, 1161
Alw26I GTCTC 2 cut(s) 191, 1034
AlwI GGATC 3 cut(s) 235, 358, 1237
Ama87I CYCGRG 1 cut(s) 784
AoxI GGCC 5 cut(s) 86, 370, 878, 991, 1308
ApeKI GCWGC 4 cut(s) 74, 638, 892, 1349
ApoI RAATTY 1 cut(s) 1220
ArsI GACNNNNNNTTYG 2 cut(s) 1136, 1168
AsiGI ACCGGT 2 cut(s) 1191, 1419
AspS9I GGNCC 6 cut(s) 86, 548, 773, 868, 1308, 1403
AsuHPI GGTGA 2 cut(s) 16, 1247
AvaI CYCGRG 1 cut(s) 784
AvaII GGWCC 4 cut(s) 548, 773, 868, 1403
AxyI CCTNAGG 1 cut(s) 1026
BalI TGGCCA 1 cut(s) 880
BbsI GAAGAC 3 cut(s) 99, 1188, 1349
BbvI GCAGC 4 cut(s) 61, 625, 904, 1361
BccI CCATC 7 cut(s) 35, 152, 251, 374, 539, 870, 889
BceAI ACGGC 2 cut(s) 1283, 1295
BcgI CGANNNNNNTGC 2 cut(s) 251, 285
BciT130I CCWGG 4 cut(s) 665, 873, 975, 1208
BcoDI GTCTC 2 cut(s) 191, 1034
BfmI CTRYAG 3 cut(s) 81, 538, 1215
BfuAI ACCTGC 2 cut(s) 1343, 1386
BglII AGATCT 1 cut(s) 1241
BisI GCNGC 5 cut(s) 75, 132, 639, 893, 1350
BlsI GCNGC 5 cut(s) 76, 133, 640, 894, 1351
Bme1390I CCNGG 4 cut(s) 665, 873, 975, 1208
Bme18I GGWCC 4 cut(s) 548, 773, 868, 1403
BmeT110I CYCGRG 1 cut(s) 784
BmgT120I GGNCC 6 cut(s) 86, 548, 773, 868, 1308, 1403
BmiI GGNNCC 3 cut(s) 550, 870, 1309
BmrFI CCNGG 4 cut(s) 665, 873, 975, 1208
BmrI ACTGGG 1 cut(s) 546
BmsI GCATC 5 cut(s) 143, 385, 550, 1011, 1279
BmuI ACTGGG 1 cut(s) 546
BpiI GAAGAC 3 cut(s) 99, 1188, 1349
BpmI CTGGAG 1 cut(s) 828
BsaBI GATNNNNATC 1 cut(s) 880
BsaI GGTCTC 1 cut(s) 1034
BsaJI CCNNGG 2 cut(s) 426, 871
BsaWI WCCGGW 2 cut(s) 1191, 1419
BsaXI ACNNNNNCTCC 4 cut(s) 1057, 1087, 1284, 1314
Bsc4I CCNNNNNNNGG 1 cut(s) 887
Bse118I RCCGGY 2 cut(s) 1191, 1419
Bse1I ACTGG 2 cut(s) 552, 845
Bse21I CCTNAGG 1 cut(s) 1026
Bse3DI GCAATG 2 cut(s) 570, 835
Bse8I GATNNNNATC 1 cut(s) 880
BseBI CCWGG 4 cut(s) 665, 873, 975, 1208
BseDI CCNNGG 2 cut(s) 426, 871
BseGI GGATG 7 cut(s) 134, 262, 409, 436, 550, 881, 1026
BseJI GATNNNNATC 1 cut(s) 880
BseLI CCNNNNNNNGG 1 cut(s) 887
BseMI GCAATG 2 cut(s) 570, 835
BseMII CTCAG 3 cut(s) 1017, 1029, 1030
BseNI ACTGG 2 cut(s) 552, 845
BseRI GAGGAG 4 cut(s) 300, 1043, 1065, 1405
BseXI GCAGC 4 cut(s) 61, 625, 904, 1361
BshFI GGCC 5 cut(s) 88, 372, 880, 993, 1310
BshTI ACCGGT 2 cut(s) 1191, 1419
BsiHKCI CYCGRG 1 cut(s) 784
BsiSI CCGG 2 cut(s) 1192, 1420
BslFI GGGAC 3 cut(s) 304, 1081, 1330
BslI CCNNNNNNNGG 1 cut(s) 887
BsmAI GTCTC 2 cut(s) 191, 1034
BsmFI GGGAC 3 cut(s) 304, 1081, 1330
BsnI GGCC 5 cut(s) 88, 372, 880, 993, 1310
Bso31I GGTCTC 1 cut(s) 1034
BsoBI CYCGRG 1 cut(s) 784
Bsp143I GATC 5 cut(s) 240, 350, 904, 1229, 1241
Bsp19I CCATGG 1 cut(s) 426
BspACI CCGC 4 cut(s) 131, 138, 244, 314
BspANI GGCC 5 cut(s) 88, 372, 880, 993, 1310
BspCNI CTCAG 3 cut(s) 1018, 1029, 1030
BspLI GGNNCC 3 cut(s) 550, 870, 1309
BspMAI CTGCAG 2 cut(s) 85, 1219
BspMI ACCTGC 2 cut(s) 1343, 1386
BspPI GGATC 3 cut(s) 235, 358, 1237
BspTNI GGTCTC 1 cut(s) 1034
BsrDI GCAATG 2 cut(s) 570, 835
BsrFI RCCGGY 2 cut(s) 1191, 1419
BsrI ACTGG 2 cut(s) 552, 845
BssAI RCCGGY 2 cut(s) 1191, 1419
BssECI CCNNGG 2 cut(s) 426, 871
BssMI GATC 5 cut(s) 240, 350, 904, 1229, 1241
BssT1I CCWWGG 1 cut(s) 426
Bst2UI CCWGG 4 cut(s) 665, 873, 975, 1208
Bst4CI ACNGT 3 cut(s) 43, 202, 689
Bst6I CTCTTC 2 cut(s) 703, 1280
BstC8I GCNNGC 4 cut(s) 535, 755, 826, 1187
BstDEI CTNAG 6 cut(s) 190, 644, 1016, 1026, 1038, 1245
BstDSI CCRYGG 1 cut(s) 426
BstF5I GGATG 7 cut(s) 134, 262, 409, 436, 550, 881, 1026
BstKTI GATC 5 cut(s) 243, 353, 907, 1232, 1244
BstMAI GTCTC 2 cut(s) 191, 1034
BstMBI GATC 5 cut(s) 240, 350, 904, 1229, 1241
BstMWI GCNNNNNNNGC 4 cut(s) 74, 80, 137, 1186
BstNI CCWGG 4 cut(s) 665, 873, 975, 1208
BstNSI RCATGY 3 cut(s) 753, 757, 824
BstSCI CCNGG 4 cut(s) 663, 871, 973, 1206
BstSFI CTRYAG 3 cut(s) 81, 538, 1215
BstV1I GCAGC 4 cut(s) 61, 625, 904, 1361
BstV2I GAAGAC 3 cut(s) 99, 1188, 1349
BstX2I RGATCY 3 cut(s) 240, 1229, 1241
BstXI CCANNNNNNTGG 2 cut(s) 158, 380
BstYI RGATCY 3 cut(s) 240, 1229, 1241
Bsu36I CCTNAGG 1 cut(s) 1026
BsuRI GGCC 5 cut(s) 88, 372, 880, 993, 1310
BtgI CCRYGG 1 cut(s) 426
BtgZI GCGATG 1 cut(s) 1256
BtsCI GGATG 7 cut(s) 134, 262, 409, 436, 550, 881, 1026
BtsI GCAGTG 1 cut(s) 936
BtsIMutI CAGTG 2 cut(s) 559, 936
BveI ACCTGC 2 cut(s) 1343, 1386
Cac8I GCNNGC 4 cut(s) 535, 755, 826, 1187
Cfr10I RCCGGY 2 cut(s) 1191, 1419
Cfr13I GGNCC 6 cut(s) 86, 548, 773, 868, 1308, 1403
Csp6I GTAC 2 cut(s) 187, 685
CspAI ACCGGT 2 cut(s) 1191, 1419
CspCI CAANNNNNGTGG 2 cut(s) 829, 864
CviAII CATG 8 cut(s) 71, 374, 427, 750, 754, 821, 970, 1339
CviQI GTAC 2 cut(s) 187, 685
DdeI CTNAG 6 cut(s) 190, 644, 1016, 1026, 1038, 1245
DpnI GATC 5 cut(s) 242, 352, 906, 1231, 1243
DpnII GATC 5 cut(s) 240, 350, 904, 1229, 1241
DraI TTTAAA 1 cut(s) 1165
EaeI YGGCCR 1 cut(s) 878
Eam1104I CTCTTC 2 cut(s) 703, 1280
EarI CTCTTC 2 cut(s) 703, 1280
EciI GGCGGA 1 cut(s) 127
Eco130I CCWWGG 1 cut(s) 426
Eco147I AGGCCT 1 cut(s) 993
Eco31I GGTCTC 1 cut(s) 1034
Eco32I GATATC 1 cut(s) 654
Eco47I GGWCC 4 cut(s) 548, 773, 868, 1403
Eco57I CTGAAG 3 cut(s) 743, 783, 932
Eco81I CCTNAGG 1 cut(s) 1026
Eco88I CYCGRG 1 cut(s) 784
EcoO109I RGGNCCY 1 cut(s) 868
EcoRI GAATTC 1 cut(s) 1220
EcoRII CCWGG 4 cut(s) 663, 871, 973, 1206
EcoRV GATATC 1 cut(s) 654
EcoT14I CCWWGG 1 cut(s) 426
EcoT22I ATGCAT 1 cut(s) 755
ErhI CCWWGG 1 cut(s) 426
FaeI CATG 8 cut(s) 74, 377, 430, 753, 757, 824, 973, 1342
FaqI GGGAC 3 cut(s) 304, 1081, 1330
FatI CATG 8 cut(s) 70, 373, 426, 749, 753, 820, 969, 1338
Fnu4HI GCNGC 5 cut(s) 75, 132, 639, 893, 1350
FokI GGATG 7 cut(s) 121, 269, 396, 443, 557, 888, 1033
Fsp4HI GCNGC 5 cut(s) 75, 132, 639, 893, 1350
GluI GCNGC 5 cut(s) 75, 132, 639, 893, 1350
GsuI CTGGAG 1 cut(s) 828
HaeIII GGCC 5 cut(s) 88, 372, 880, 993, 1310
HapII CCGG 2 cut(s) 1192, 1420
Hin1II CATG 8 cut(s) 74, 377, 430, 753, 757, 824, 973, 1342
HinfI GANTC 5 cut(s) 278, 466, 1010, 1211, 1322
HpaII CCGG 2 cut(s) 1192, 1420
HphI GGTGA 2 cut(s) 16, 1247
Hpy166II GTNNAC 4 cut(s) 123, 277, 1054, 1336
Hpy188I TCNGA 7 cut(s) 157, 253, 303, 802, 1084, 1129, 1368
Hpy188III TCNNGA 5 cut(s) 238, 733, 784, 786, 1018
Hpy8I GTNNAC 4 cut(s) 123, 277, 1054, 1336
HpyAV CCTTC 2 cut(s) 203, 807
HpyCH4III ACNGT 3 cut(s) 43, 202, 689
HpyCH4V TGCA 7 cut(s) 83, 563, 753, 824, 912, 1112, 1217
HpyF10VI GCNNNNNNNGC 4 cut(s) 74, 80, 137, 1186
HpyF3I CTNAG 6 cut(s) 190, 644, 1016, 1026, 1038, 1245
Hsp92II CATG 8 cut(s) 74, 377, 430, 753, 757, 824, 973, 1342
Kzo9I GATC 5 cut(s) 240, 350, 904, 1229, 1241
LmnI GCTCC 3 cut(s) 82, 1078, 1392
Lsp1109I GCAGC 4 cut(s) 61, 625, 904, 1361
LweI GCATC 5 cut(s) 143, 385, 550, 1011, 1279
MaeIII GTNAC 4 cut(s) 31, 421, 499, 931
MalI GATC 5 cut(s) 242, 352, 906, 1231, 1243
MboI GATC 5 cut(s) 240, 350, 904, 1229, 1241
MflI RGATCY 3 cut(s) 240, 1229, 1241
MlsI TGGCCA 1 cut(s) 880
MluCI AATT 4 cut(s) 59, 483, 900, 1220
MluNI TGGCCA 1 cut(s) 880
MlyI GAGTC 1 cut(s) 272
Mox20I TGGCCA 1 cut(s) 880
Mph1103I ATGCAT 1 cut(s) 755
MscI TGGCCA 1 cut(s) 880
MseI TTAA 4 cut(s) 285, 1004, 1164, 1426
MslI CAYNNNNRTG 2 cut(s) 378, 752
Msp20I TGGCCA 1 cut(s) 880
MspA1I CMGCKG 1 cut(s) 316
MspI CCGG 2 cut(s) 1192, 1420
MspR9I CCNGG 4 cut(s) 665, 873, 975, 1208
MvaI CCWGG 4 cut(s) 665, 873, 975, 1208
MwoI GCNNNNNNNGC 4 cut(s) 74, 80, 137, 1186
NcoI CCATGG 1 cut(s) 426
NdeII GATC 5 cut(s) 240, 350, 904, 1229, 1241
NlaIII CATG 8 cut(s) 74, 377, 430, 753, 757, 824, 973, 1342
NlaIV GGNNCC 3 cut(s) 550, 870, 1309
NmeAIII GCCGAG 1 cut(s) 1069
NmuCI GTSAC 3 cut(s) 31, 499, 931
NsiI ATGCAT 1 cut(s) 755
NspI RCATGY 3 cut(s) 753, 757, 824
PaeI GCATGC 1 cut(s) 757
PaeR7I CTCGAG 1 cut(s) 784
PceI AGGCCT 1 cut(s) 993
PfeI GAWTC 4 cut(s) 466, 1010, 1211, 1322
PflMI CCANNNNNTGG 1 cut(s) 887
PinAI ACCGGT 2 cut(s) 1191, 1419
PkrI GCNGC 5 cut(s) 76, 133, 640, 894, 1351
PleI GAGTC 1 cut(s) 272
PpsI GAGTC 1 cut(s) 272
PpuMI RGGWCCY 1 cut(s) 868
PsiI TTATAA 1 cut(s) 899
Psp5II RGGWCCY 1 cut(s) 868
Psp6I CCWGG 4 cut(s) 663, 871, 973, 1206
PspGI CCWGG 4 cut(s) 663, 871, 973, 1206
PspN4I GGNNCC 3 cut(s) 550, 870, 1309
PspPI GGNCC 6 cut(s) 86, 548, 773, 868, 1308, 1403
PspPPI RGGWCCY 1 cut(s) 868
PstI CTGCAG 2 cut(s) 85, 1219
PsuI RGATCY 3 cut(s) 240, 1229, 1241
RsaI GTAC 2 cut(s) 188, 686
RsaNI GTAC 2 cut(s) 187, 685
RseI CAYNNNNRTG 2 cut(s) 378, 752
SaqAI TTAA 4 cut(s) 285, 1004, 1164, 1426
SatI GCNGC 5 cut(s) 75, 132, 639, 893, 1350
Sau3AI GATC 5 cut(s) 240, 350, 904, 1229, 1241
Sau96I GGNCC 6 cut(s) 86, 548, 773, 868, 1308, 1403
SbfI CCTGCAGG 1 cut(s) 85
SchI GAGTC 1 cut(s) 272
ScrFI CCNGG 4 cut(s) 665, 873, 975, 1208
SdaI CCTGCAGG 1 cut(s) 85
SfaNI GCATC 5 cut(s) 143, 385, 550, 1011, 1279
SfcI CTRYAG 3 cut(s) 81, 538, 1215
Sfr274I CTCGAG 1 cut(s) 784
SinI GGWCC 4 cut(s) 548, 773, 868, 1403
SlaI CTCGAG 1 cut(s) 784
SmiMI CAYNNNNRTG 2 cut(s) 378, 752
SmlI CTYRAG 1 cut(s) 784
SmoI CTYRAG 1 cut(s) 784
SphI GCATGC 1 cut(s) 757
Sse8387I CCTGCAGG 1 cut(s) 85
Sse9I AATT 4 cut(s) 59, 483, 900, 1220
SseBI AGGCCT 1 cut(s) 993
SsiI CCGC 4 cut(s) 131, 138, 244, 314
SspI AATATT 1 cut(s) 529
StuI AGGCCT 1 cut(s) 993
StyD4I CCNGG 4 cut(s) 663, 871, 973, 1206
StyI CCWWGG 1 cut(s) 426
TaaI ACNGT 3 cut(s) 43, 202, 689
TaqI TCGA 2 cut(s) 785, 1325
TasI AATT 4 cut(s) 59, 483, 900, 1220
TatI WGTACW 1 cut(s) 684
TauI GCSGC 1 cut(s) 134
TfiI GAWTC 4 cut(s) 466, 1010, 1211, 1322
Tru1I TTAA 4 cut(s) 285, 1004, 1164, 1426
Tru9I TTAA 4 cut(s) 285, 1004, 1164, 1426
TscAI CASTG 2 cut(s) 559, 936
TseFI GTSAC 3 cut(s) 31, 499, 931
TseI GCWGC 4 cut(s) 74, 638, 892, 1349
Tsp45I GTSAC 3 cut(s) 31, 499, 931
TspDTI ATGAA 6 cut(s) 10, 846, 943, 958, 1148, 1355
TspGWI ACGGA 1 cut(s) 271
TspRI CASTG 2 cut(s) 559, 936
Van91I CCANNNNNTGG 1 cut(s) 887
VpaK11BI GGWCC 4 cut(s) 548, 773, 868, 1403
XapI RAATTY 1 cut(s) 1220
XceI RCATGY 3 cut(s) 753, 757, 824
XcmI CCANNNNNNNNNTGG 1 cut(s) 847
XhoI CTCGAG 1 cut(s) 784
Zsp2I ATGCAT 1 cut(s) 755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.