Rh5DG214300

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0010007
Physical Location & Seq
Reverse (-)
24237870 .. 24242737
4868 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG214300.1

Sequence Viewer

Length: 1395 bp
ATGACAAGTTCCCTTTTCTTCATCACCATCGTCACTCTCACAGTCGCTTACACCCTCCAATTCAAAGCCCATGCTGCTCCTGCAGGGCCATTGATAAAGCACTTGTCTTCCCTTGTCAAGTGGACGAGGGCGGCATCCGCCAAAGCACCCCAATCAGATGGGAATGTTGTTCAGTTTGAGAGTGGGTACTTAGTTGAGACTGTTGTGGAAGGAACTGATATTGGAGTTGTTCCTTTCAAGATCCGCATTTCAGAGGATGGCGAACTCTTTGCTGTGGACTCCGTTAATAGCAACATTGTTCGGATTACTCCTCCGCTGTCCCAATATAGTAGGGGAAGATTGGTTGCTGGATCATTTCAAGGTTATACAGGCCATGTTGATGGAAAGCCAAGTGATGCTCGTTTCAATCATCCAAAAGGTGTAACCATGGATGATAAAGGGAATGTATATGTTGCTGATACCTTGAATCACGCCATAAGAAAAATTGGAGAAGCTGGTGTGACAACCATAGCTGGTGGAAAATCAAATATTGCTGGCTACAGGGATGGACCCAGTGAAGATGCAATGTTCTCAAACGATTTTGATGTAGTATATGTCCAGCCTACTTGTTCCTTGCTTGTTGTTGATAGAGGAAATGCTGCTCTTAGGCAGATATCTCTCAACCAGGAGGATTGTGATTATCAGTACAGTTCAATCTCTTCCACAGGCTATGCCACATGCATGCTTCAGCAGGGTTTTGGACCTTTCTTCTCGAGAACGCAACCTTCAGAGAGTGAAACTAAAGAACATGCAAGCAATGAAAAATCCACTCCAGTTGTGGAGATTGTGAAAGAGGACCCAGGATGGCCATCTTTTGGGCAGCTTATAATTGATCTATGCAAGATTGGACTTGAAGCAGTGACAGGCACATTTCTTCAGTTCATTCCCTCTCGGTTCATGCCTGGTGGTTCTCACAAAGGCCTGACACCATTAAAAGATTCTCTCAGGATGCCTGAGGAGGAAGCTGAGACCCCGTTAGTTCACAGGCAAAGCACTCCTGCTCCTCTGTCTGAAACTCGGCAGTCCCATACTCCCAATGCAAGTGATAGATATTCAGATATGAAGCCCTCAAAGATAAAGTCAAGTAGCTTTAAAGACCCCTCTTTGCCAAGCAAGCACCGGTCTTCAAAACGCCAGGATTCTGCAGAATTCTATGGATCTGGTGAAAGATCTAAGAGCCAGAAAGAAAGAACACGGCATCGCCAACGAGAGAAGAGTGGAGAGGTAGTTTTTGGGGCCGTTGGGACAGAATCGAAACCTGTGGACATGAAGACAGCAGCAGGTTATGACAATCAGAAGTTTGAGAACTACAATATGAGGAGCAGGTATGGACCTGACAAGTCATACCGGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NHL

Protein Analysis

464

Amino Acids

50.96

Weight (kDa)

8.16

Isoelectric Point (pI)

38.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NHL PF01436 135 - 161 5.8e-06 NHL repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 866
Acc36I ACCTGC 2 cut(s) 1310, 1353
AccB7I CCANNNNNTGG 1 cut(s) 854
AciI CCGC 4 cut(s) 131, 138, 244, 314
AclWI GGATC 3 cut(s) 235, 358, 1204
AcoI YGGCCR 1 cut(s) 845
AcsI RAATTY 1 cut(s) 1187
AcuI CTGAAG 3 cut(s) 710, 750, 899
AfaI GTAC 2 cut(s) 188, 686
AfiI CCNNNNNNNGG 1 cut(s) 854
AgeI ACCGGT 2 cut(s) 1158, 1386
AgsI TTSAA 8 cut(s) 64, 238, 359, 406, 466, 693, 893, 1167
AjnI CCWGG 4 cut(s) 663, 838, 940, 1173
AluBI AGCT 5 cut(s) 494, 512, 862, 1004, 1128
AluI AGCT 5 cut(s) 494, 512, 862, 1004, 1128
Alw26I GTCTC 2 cut(s) 191, 1001
AlwI GGATC 3 cut(s) 235, 358, 1204
Ama87I CYCGRG 1 cut(s) 751
AoxI GGCC 5 cut(s) 86, 370, 845, 958, 1275
ApeKI GCWGC 4 cut(s) 74, 638, 859, 1316
ApoI RAATTY 1 cut(s) 1187
ArsI GACNNNNNNTTYG 2 cut(s) 1103, 1135
AsiGI ACCGGT 2 cut(s) 1158, 1386
AspS9I GGNCC 6 cut(s) 86, 548, 740, 835, 1275, 1370
AsuHPI GGTGA 2 cut(s) 16, 1214
AvaI CYCGRG 1 cut(s) 751
AvaII GGWCC 4 cut(s) 548, 740, 835, 1370
AxyI CCTNAGG 1 cut(s) 993
BalI TGGCCA 1 cut(s) 847
BbsI GAAGAC 3 cut(s) 99, 1155, 1316
BbvI GCAGC 4 cut(s) 61, 625, 871, 1328
BccI CCATC 7 cut(s) 35, 152, 251, 374, 539, 837, 856
BceAI ACGGC 2 cut(s) 1250, 1262
BcgI CGANNNNNNTGC 2 cut(s) 251, 285
BciT130I CCWGG 4 cut(s) 665, 840, 942, 1175
BcoDI GTCTC 2 cut(s) 191, 1001
BfmI CTRYAG 3 cut(s) 81, 538, 1182
BfuAI ACCTGC 2 cut(s) 1310, 1353
BglII AGATCT 1 cut(s) 1208
BisI GCNGC 5 cut(s) 75, 132, 639, 860, 1317
BlsI GCNGC 5 cut(s) 76, 133, 640, 861, 1318
Bme1390I CCNGG 4 cut(s) 665, 840, 942, 1175
Bme18I GGWCC 4 cut(s) 548, 740, 835, 1370
BmeT110I CYCGRG 1 cut(s) 751
BmgT120I GGNCC 6 cut(s) 86, 548, 740, 835, 1275, 1370
BmiI GGNNCC 3 cut(s) 550, 837, 1276
BmrFI CCNGG 4 cut(s) 665, 840, 942, 1175
BmrI ACTGGG 1 cut(s) 546
BmsI GCATC 5 cut(s) 143, 385, 550, 978, 1246
BmuI ACTGGG 1 cut(s) 546
BpiI GAAGAC 3 cut(s) 99, 1155, 1316
BpmI CTGGAG 1 cut(s) 795
BsaBI GATNNNNATC 1 cut(s) 847
BsaI GGTCTC 1 cut(s) 1001
BsaJI CCNNGG 2 cut(s) 426, 838
BsaWI WCCGGW 2 cut(s) 1158, 1386
BsaXI ACNNNNNCTCC 4 cut(s) 1024, 1054, 1251, 1281
Bsc4I CCNNNNNNNGG 1 cut(s) 854
Bse118I RCCGGY 2 cut(s) 1158, 1386
Bse1I ACTGG 2 cut(s) 552, 812
Bse21I CCTNAGG 1 cut(s) 993
Bse3DI GCAATG 2 cut(s) 570, 802
Bse8I GATNNNNATC 1 cut(s) 847
BseBI CCWGG 4 cut(s) 665, 840, 942, 1175
BseDI CCNNGG 2 cut(s) 426, 838
BseGI GGATG 7 cut(s) 134, 262, 409, 436, 550, 848, 993
BseJI GATNNNNATC 1 cut(s) 847
BseLI CCNNNNNNNGG 1 cut(s) 854
BseMI GCAATG 2 cut(s) 570, 802
BseMII CTCAG 3 cut(s) 984, 996, 997
BseNI ACTGG 2 cut(s) 552, 812
BseRI GAGGAG 4 cut(s) 300, 1010, 1032, 1372
BseXI GCAGC 4 cut(s) 61, 625, 871, 1328
BshFI GGCC 5 cut(s) 88, 372, 847, 960, 1277
BshTI ACCGGT 2 cut(s) 1158, 1386
BsiHKCI CYCGRG 1 cut(s) 751
BsiSI CCGG 2 cut(s) 1159, 1387
BslFI GGGAC 3 cut(s) 304, 1048, 1297
BslI CCNNNNNNNGG 1 cut(s) 854
BsmAI GTCTC 2 cut(s) 191, 1001
BsmFI GGGAC 3 cut(s) 304, 1048, 1297
BsnI GGCC 5 cut(s) 88, 372, 847, 960, 1277
Bso31I GGTCTC 1 cut(s) 1001
BsoBI CYCGRG 1 cut(s) 751
Bsp143I GATC 5 cut(s) 240, 350, 871, 1196, 1208
Bsp19I CCATGG 1 cut(s) 426
BspACI CCGC 4 cut(s) 131, 138, 244, 314
BspANI GGCC 5 cut(s) 88, 372, 847, 960, 1277
BspCNI CTCAG 3 cut(s) 985, 996, 997
BspLI GGNNCC 3 cut(s) 550, 837, 1276
BspMAI CTGCAG 2 cut(s) 85, 1186
BspMI ACCTGC 2 cut(s) 1310, 1353
BspPI GGATC 3 cut(s) 235, 358, 1204
BspTNI GGTCTC 1 cut(s) 1001
BsrDI GCAATG 2 cut(s) 570, 802
BsrFI RCCGGY 2 cut(s) 1158, 1386
BsrI ACTGG 2 cut(s) 552, 812
BssAI RCCGGY 2 cut(s) 1158, 1386
BssECI CCNNGG 2 cut(s) 426, 838
BssMI GATC 5 cut(s) 240, 350, 871, 1196, 1208
BssT1I CCWWGG 1 cut(s) 426
Bst2UI CCWGG 4 cut(s) 665, 840, 942, 1175
Bst4CI ACNGT 3 cut(s) 43, 202, 689
Bst6I CTCTTC 2 cut(s) 703, 1247
BstC8I GCNNGC 4 cut(s) 535, 722, 793, 1154
BstDEI CTNAG 6 cut(s) 190, 644, 983, 993, 1005, 1212
BstDSI CCRYGG 1 cut(s) 426
BstF5I GGATG 7 cut(s) 134, 262, 409, 436, 550, 848, 993
BstKTI GATC 5 cut(s) 243, 353, 874, 1199, 1211
BstMAI GTCTC 2 cut(s) 191, 1001
BstMBI GATC 5 cut(s) 240, 350, 871, 1196, 1208
BstMWI GCNNNNNNNGC 4 cut(s) 74, 80, 137, 1153
BstNI CCWGG 4 cut(s) 665, 840, 942, 1175
BstNSI RCATGY 3 cut(s) 720, 724, 791
BstSCI CCNGG 4 cut(s) 663, 838, 940, 1173
BstSFI CTRYAG 3 cut(s) 81, 538, 1182
BstV1I GCAGC 4 cut(s) 61, 625, 871, 1328
BstV2I GAAGAC 3 cut(s) 99, 1155, 1316
BstX2I RGATCY 3 cut(s) 240, 1196, 1208
BstXI CCANNNNNNTGG 2 cut(s) 158, 380
BstYI RGATCY 3 cut(s) 240, 1196, 1208
Bsu36I CCTNAGG 1 cut(s) 993
BsuRI GGCC 5 cut(s) 88, 372, 847, 960, 1277
BtgI CCRYGG 1 cut(s) 426
BtgZI GCGATG 1 cut(s) 1223
BtsCI GGATG 7 cut(s) 134, 262, 409, 436, 550, 848, 993
BtsI GCAGTG 1 cut(s) 903
BtsIMutI CAGTG 2 cut(s) 559, 903
BveI ACCTGC 2 cut(s) 1310, 1353
Cac8I GCNNGC 4 cut(s) 535, 722, 793, 1154
Cfr10I RCCGGY 2 cut(s) 1158, 1386
Cfr13I GGNCC 6 cut(s) 86, 548, 740, 835, 1275, 1370
Csp6I GTAC 2 cut(s) 187, 685
CspAI ACCGGT 2 cut(s) 1158, 1386
CspCI CAANNNNNGTGG 2 cut(s) 796, 831
CviAII CATG 8 cut(s) 71, 374, 427, 717, 721, 788, 937, 1306
CviQI GTAC 2 cut(s) 187, 685
DdeI CTNAG 6 cut(s) 190, 644, 983, 993, 1005, 1212
DpnI GATC 5 cut(s) 242, 352, 873, 1198, 1210
DpnII GATC 5 cut(s) 240, 350, 871, 1196, 1208
DraI TTTAAA 1 cut(s) 1132
EaeI YGGCCR 1 cut(s) 845
Eam1104I CTCTTC 2 cut(s) 703, 1247
EarI CTCTTC 2 cut(s) 703, 1247
EciI GGCGGA 1 cut(s) 127
Eco130I CCWWGG 1 cut(s) 426
Eco147I AGGCCT 1 cut(s) 960
Eco31I GGTCTC 1 cut(s) 1001
Eco32I GATATC 1 cut(s) 654
Eco47I GGWCC 4 cut(s) 548, 740, 835, 1370
Eco57I CTGAAG 3 cut(s) 710, 750, 899
Eco81I CCTNAGG 1 cut(s) 993
Eco88I CYCGRG 1 cut(s) 751
EcoO109I RGGNCCY 1 cut(s) 835
EcoRI GAATTC 1 cut(s) 1187
EcoRII CCWGG 4 cut(s) 663, 838, 940, 1173
EcoRV GATATC 1 cut(s) 654
EcoT14I CCWWGG 1 cut(s) 426
EcoT22I ATGCAT 1 cut(s) 722
ErhI CCWWGG 1 cut(s) 426
FaeI CATG 8 cut(s) 74, 377, 430, 720, 724, 791, 940, 1309
FaqI GGGAC 3 cut(s) 304, 1048, 1297
FatI CATG 8 cut(s) 70, 373, 426, 716, 720, 787, 936, 1305
Fnu4HI GCNGC 5 cut(s) 75, 132, 639, 860, 1317
FokI GGATG 7 cut(s) 121, 269, 396, 443, 557, 855, 1000
Fsp4HI GCNGC 5 cut(s) 75, 132, 639, 860, 1317
GluI GCNGC 5 cut(s) 75, 132, 639, 860, 1317
GsuI CTGGAG 1 cut(s) 795
HaeIII GGCC 5 cut(s) 88, 372, 847, 960, 1277
HapII CCGG 2 cut(s) 1159, 1387
Hin1II CATG 8 cut(s) 74, 377, 430, 720, 724, 791, 940, 1309
HinfI GANTC 5 cut(s) 278, 466, 977, 1178, 1289
HpaII CCGG 2 cut(s) 1159, 1387
HphI GGTGA 2 cut(s) 16, 1214
Hpy166II GTNNAC 4 cut(s) 123, 277, 1021, 1303
Hpy188I TCNGA 7 cut(s) 157, 253, 303, 769, 1051, 1096, 1335
Hpy188III TCNNGA 4 cut(s) 238, 751, 753, 985
Hpy8I GTNNAC 4 cut(s) 123, 277, 1021, 1303
HpyAV CCTTC 2 cut(s) 203, 774
HpyCH4III ACNGT 3 cut(s) 43, 202, 689
HpyCH4V TGCA 7 cut(s) 83, 563, 720, 791, 879, 1079, 1184
HpyF10VI GCNNNNNNNGC 4 cut(s) 74, 80, 137, 1153
HpyF3I CTNAG 6 cut(s) 190, 644, 983, 993, 1005, 1212
Hsp92II CATG 8 cut(s) 74, 377, 430, 720, 724, 791, 940, 1309
Kzo9I GATC 5 cut(s) 240, 350, 871, 1196, 1208
LmnI GCTCC 3 cut(s) 82, 1045, 1359
Lsp1109I GCAGC 4 cut(s) 61, 625, 871, 1328
LweI GCATC 5 cut(s) 143, 385, 550, 978, 1246
MaeIII GTNAC 4 cut(s) 31, 421, 499, 898
MalI GATC 5 cut(s) 242, 352, 873, 1198, 1210
MboI GATC 5 cut(s) 240, 350, 871, 1196, 1208
MflI RGATCY 3 cut(s) 240, 1196, 1208
MlsI TGGCCA 1 cut(s) 847
MluCI AATT 4 cut(s) 59, 483, 867, 1187
MluNI TGGCCA 1 cut(s) 847
MlyI GAGTC 1 cut(s) 272
Mox20I TGGCCA 1 cut(s) 847
Mph1103I ATGCAT 1 cut(s) 722
MscI TGGCCA 1 cut(s) 847
MseI TTAA 4 cut(s) 285, 971, 1131, 1393
MslI CAYNNNNRTG 2 cut(s) 378, 719
Msp20I TGGCCA 1 cut(s) 847
MspA1I CMGCKG 1 cut(s) 316
MspI CCGG 2 cut(s) 1159, 1387
MspR9I CCNGG 4 cut(s) 665, 840, 942, 1175
MvaI CCWGG 4 cut(s) 665, 840, 942, 1175
MwoI GCNNNNNNNGC 4 cut(s) 74, 80, 137, 1153
NcoI CCATGG 1 cut(s) 426
NdeII GATC 5 cut(s) 240, 350, 871, 1196, 1208
NlaIII CATG 8 cut(s) 74, 377, 430, 720, 724, 791, 940, 1309
NlaIV GGNNCC 3 cut(s) 550, 837, 1276
NmeAIII GCCGAG 1 cut(s) 1036
NmuCI GTSAC 3 cut(s) 31, 499, 898
NsiI ATGCAT 1 cut(s) 722
NspI RCATGY 3 cut(s) 720, 724, 791
PaeI GCATGC 1 cut(s) 724
PaeR7I CTCGAG 1 cut(s) 751
PceI AGGCCT 1 cut(s) 960
PfeI GAWTC 4 cut(s) 466, 977, 1178, 1289
PflMI CCANNNNNTGG 1 cut(s) 854
PinAI ACCGGT 2 cut(s) 1158, 1386
PkrI GCNGC 5 cut(s) 76, 133, 640, 861, 1318
PleI GAGTC 1 cut(s) 272
PpsI GAGTC 1 cut(s) 272
PpuMI RGGWCCY 1 cut(s) 835
PsiI TTATAA 1 cut(s) 866
Psp5II RGGWCCY 1 cut(s) 835
Psp6I CCWGG 4 cut(s) 663, 838, 940, 1173
PspGI CCWGG 4 cut(s) 663, 838, 940, 1173
PspN4I GGNNCC 3 cut(s) 550, 837, 1276
PspPI GGNCC 6 cut(s) 86, 548, 740, 835, 1275, 1370
PspPPI RGGWCCY 1 cut(s) 835
PstI CTGCAG 2 cut(s) 85, 1186
PsuI RGATCY 3 cut(s) 240, 1196, 1208
RsaI GTAC 2 cut(s) 188, 686
RsaNI GTAC 2 cut(s) 187, 685
RseI CAYNNNNRTG 2 cut(s) 378, 719
SaqAI TTAA 4 cut(s) 285, 971, 1131, 1393
SatI GCNGC 5 cut(s) 75, 132, 639, 860, 1317
Sau3AI GATC 5 cut(s) 240, 350, 871, 1196, 1208
Sau96I GGNCC 6 cut(s) 86, 548, 740, 835, 1275, 1370
SbfI CCTGCAGG 1 cut(s) 85
SchI GAGTC 1 cut(s) 272
ScrFI CCNGG 4 cut(s) 665, 840, 942, 1175
SdaI CCTGCAGG 1 cut(s) 85
SfaNI GCATC 5 cut(s) 143, 385, 550, 978, 1246
SfcI CTRYAG 3 cut(s) 81, 538, 1182
Sfr274I CTCGAG 1 cut(s) 751
SinI GGWCC 4 cut(s) 548, 740, 835, 1370
SlaI CTCGAG 1 cut(s) 751
SmiMI CAYNNNNRTG 2 cut(s) 378, 719
SmlI CTYRAG 1 cut(s) 751
SmoI CTYRAG 1 cut(s) 751
SphI GCATGC 1 cut(s) 724
Sse8387I CCTGCAGG 1 cut(s) 85
Sse9I AATT 4 cut(s) 59, 483, 867, 1187
SseBI AGGCCT 1 cut(s) 960
SsiI CCGC 4 cut(s) 131, 138, 244, 314
SspI AATATT 1 cut(s) 529
StuI AGGCCT 1 cut(s) 960
StyD4I CCNGG 4 cut(s) 663, 838, 940, 1173
StyI CCWWGG 1 cut(s) 426
TaaI ACNGT 3 cut(s) 43, 202, 689
TaqI TCGA 2 cut(s) 752, 1292
TasI AATT 4 cut(s) 59, 483, 867, 1187
TatI WGTACW 1 cut(s) 684
TauI GCSGC 1 cut(s) 134
TfiI GAWTC 4 cut(s) 466, 977, 1178, 1289
Tru1I TTAA 4 cut(s) 285, 971, 1131, 1393
Tru9I TTAA 4 cut(s) 285, 971, 1131, 1393
TscAI CASTG 2 cut(s) 559, 903
TseFI GTSAC 3 cut(s) 31, 499, 898
TseI GCWGC 4 cut(s) 74, 638, 859, 1316
Tsp45I GTSAC 3 cut(s) 31, 499, 898
TspDTI ATGAA 6 cut(s) 10, 813, 910, 925, 1115, 1322
TspGWI ACGGA 1 cut(s) 271
TspRI CASTG 2 cut(s) 559, 903
Van91I CCANNNNNTGG 1 cut(s) 854
VpaK11BI GGWCC 4 cut(s) 548, 740, 835, 1370
XapI RAATTY 1 cut(s) 1187
XceI RCATGY 3 cut(s) 720, 724, 791
XcmI CCANNNNNNNNNTGG 1 cut(s) 814
XhoI CTCGAG 1 cut(s) 751
Zsp2I ATGCAT 1 cut(s) 722
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.